WO2006128335A1

WO2006128335A1 - Echinacea honey and its oral preparation

Info

Publication number: WO2006128335A1
Application number: PCT/CN2005/001756
Authority: WO
Inventors: Hongping Yie; Zuolin Zhu; Meg M. Sun
Original assignee: Pficker Pharmaceutical Ltd.
Priority date: 2005-05-28
Filing date: 2005-10-24
Publication date: 2006-12-07
Also published as: CN1778315A

Abstract

The invention disclosed a kind of Echinacea honey and its oral preparation. The honey and the preparation comprise certain amount of pinocembrin and phenolics, and have improved curing effects and healthy function over the Echinacea juice, concentrate of Echinacea extracts or normal honey. And also provided method for preparing them.

Description

Acenesian honey and its oral preparations

The invention relates to the field of health food technology. Specifically, it relates to an Aiconia honey and an oral preparation thereof and a process for the preparation thereof. Background technique:

Echinacea (Echinacea, also translated as Echinacea) is native to North America. Native Americans have traditionally been used as herbal medicines. They are the best medicine for treating trauma, snake bites, headaches and colds. They are generally used to prevent colds, sore throats, Influenza and wound healing, mild infection, etc., can improve immune function. It was listed in the US National Pharmacopoeia in the 1920s and is one of the most popular herbs in North America. In Europe, products sourced from Aenesia have been the number one in annual sales for several years, especially in Germany and France, where Acenesia and its preparations have become home-based medicines. In the US medicinal plant sales rankings, Aikensia ranked first in 1995, 1996, 1997, 1999, 2002, and was named 2002 in Fengyun Herbs. Its immunological activity has received widespread attention at home and abroad. Europe and the United States are widely used in medicine, nutritional supplements and health foods.

Modern medical research has found that Aikenia contains a variety of polysaccharides and glycoproteins, caffeic acid derivatives, and echinacea. For example, the polysaccharide PSI is 4-methoxy-glucaldehyde-arabinose-xylanan (4-10-methy-glucuronoar-binoxylan, Mr 35000), and the polysaccharide PS II is acidic arabinose-rhamnose-half Lactose (acidicarabinorhamnogalactan, Mr 45 000), neutral fucose-galactose-xylan-glucose (fucogalactoxyloglucans, Mr 10000 and 25,000); acidic arabinose-galactosidan

(arabinogalac tan, Mr 75000) and so on. There are three kinds of glycoproteins obtained from Akonica, Mr. 17000, 21000, and 30,000 respectively. Other compounds isolated from Akonica include fatty amides, methyl cichoric acid, and various other phenolic resins, and the like. All of these substances have been shown to have good immunity and anti-inflammatory effects (Echinacea, The Plant That Boots Your Immune System, London, 1999, Souvenir Press Ltd.).

In the past two or three decades, clinical trials have demonstrated that Akonean has a good therapeutic effect and drug safety from the perspective of modern medicine, including the treatment of natural immune system deficiency diseases (Z. Phytother. 1989, 10, 67-70. and Phytomed. 1996, 3, 95-102). Aenenia's application in modern medicine is mainly focused on its strengthening of the immune system, mainly for the treatment of viral infections, bacterial infections, protozoal diseases, And fungal infections, etc. (Alternative Medicine Review, 2001, 6, 411-414), in which clinical trials and in vitro tests have demonstrated that Acenesia juice stimulates white blood cell production and increases leukocyte phagocytic activity (J. Altern. Complement Med 1995, 1, 145-160). In clinical trials with more than 3,900 people, Acenesia juice is a good way to reduce the incidence of colds and flu, and to alleviate the symptoms of patients (Nutrition Science News, September 1999 and March 1999). In addition, it was found that Acenesia juice can effectively inhibit the division of the virus and control the degeneration of the virus (Planta Med. 1978, 33, 89-102). A retrospective study of 1,280 children with bronchitis showed that treatment with Acenesia juice can speed up the healing process compared with antibiotics, mainly because bronchitis is mostly a viral infection, and antibiotics are powerless. However, the Acenesian formulation stimulates non-specific defense mechanisms and attacks cells infected with the virus. Also found that using Ekney

'The incidence of recurrence of children with upper respiratory tract infections treated with West Asia juice is much lower than that of the blank control group (Journal of the American Medical Association, 2003, 290, 2824-2830).

The "Shen Nong's Herbal Classic", which was published in the Han Dynasty, has listed honey as a top-grade drug. Since then, there have been records of honey in the medical and herbal books of the past. Li Shizhen, a medical scientist of the Ming Dynasty, said in the "Compendium of Materia Medica": "Honey, its merits in medicine are five: heat, but also in the middle, detoxification, moistening, and pain."

> Modern scientific research and use results show that honey contains a large variety of active ingredients, such as polysaccharides, high levels of reducing sugars, glucose oxidase, lysozymes, various terpenes Terpenes, pinostrobin, pinocembrin (sometimes translated as pinein), chrysin, various phenolic resins, benzyl alcohol, and the like. Honey not only can skin care and beauty, but also promote longevity. It also has good antibacterial and anti-inflammatory properties, promotes tissue regeneration, promotes digestion, improves immunity, improves sleep, protects the liver, and resists

0 Fatigue, promote children's growth and development, protect cardiovascular, lungs and cough, promote calcium absorption and other important functions. In addition, honey is a strong antioxidant.

Pinocembrin contained in honey, its antibacterial and antiviral, has strong inhibitory effect on Gram-positive bacteria such as streptococcus, staphylococcus, diphtheria, etc., and has a regulating effect on gastrointestinal function, which can make gastric acid secretion. Normal, promotes digestion, relieves oral ulcers, and accelerates wound healing. At the same time, honey contains a compound called phenolics, which is different from Acenesia: 5 juice or extract concentrate, phenolic resin. It has antioxidant properties, so the antioxidant capacity of honey is close to that of vitamins. (Although many clinical trials have shown that Avenida products such as Avenian juice can improve the body's immunity well (Altern. Med. Rev. 2002, 7, 138-149), and many compounds extracted from Afenesia are certified It has a very good effect, but the mechanism of Acenesia to improve the body's immunity is more complicated and has not been fully understood so far. However, any single compound isolated from Afenesia is not as effective as Aikencia. It is now mainly treated with Acenesia such as whole grass and juice, but oral Acenesia juice preparations are generally required. After taking it for more than ten days, it sometimes reaches 12 weeks. Although the toxicity of Acenesia juice is very low, in the long-term toxicity experiment, the juice of Acenesia is also very well tolerated, with few side effects, but the taste is not Good, it is more difficult for children under the age of 12 and the elderly. Moreover, the Econesian juice or extract concentrate itself is not stable, prone to precipitation and affects the medical effect of the product, and it is necessary to add some acid to adjust their pH to slightly acidic to stabilize.

' Summary of the content:

The main problem to be solved by the present invention is to overcome the above-mentioned deficiencies and to study the Aikenian honey product with better design effect.

The present invention provides an Acenesian honey and an oral preparation thereof.

The Arkansas honey of the present invention contains an active ingredient known in the world, such as pinocembrin and phenolics, wherein the content of the phenolic resin is the total weight of the honey. 0. 5~ 0%, the content of jossin is 200~200 (g/100g honey.

Another object of the present invention is to provide an oral preparation of Acenesian honey consisting of Acenesian honey and edible excipients. The arsenic honey contains the active ingredient of Acenesia, and contains the choline and the phenolic resin, the content of the ginseng is 200~200 (^g/100g oral preparation, the content of the phenolic resin is the total weight of the honey) 0. 5~ 3. 0%.

Yet another object of the present invention is to provide a method of preparing Acenesian honey:

Method 1: Beekeeping method: The beekeeping grazing method suitable for the production of Aikenian honey has three ways: large transfer, small transfer, and fixed land. In the case of a small planting area in Aikenia, it is best to raise the ground, that is, the bee colony is basically kept in a fixed bee farm, which can be anywhere in the Akonian planting area. It is best to be placed in the central part of the Akonese growing area.

Although each species of bee has its own characteristics, the species of bees suitable for the production of Avenida honey include all bees, namely China's honey peaks and bees from western countries, such as the Italian honeybee (Apis mellifera ligustica; Italian bee). ), A. m. carnica; Carniolan bee, Carba A. m. carpathica; Carpathian bee, A. m. caucasica; Caucasian bee, also includes bees of various hybrids cultivated to increase yield, such as cultivation in a closed manner ( The Closed population breeding method introduces the genes of some excellent new bee species, strengthens the bee species and increases the gene of the bee species (dual) to reduce the number of new bee species obtained by the production of diploid drones.

Generally, the newly harvested honey has a moisture content of more than 20%. It is easy to ferment and deteriorate after long-term storage, so it must be concentrated and dehydrated. The method of concentrated dehydration can use low temperature vacuum concentration, infrared concentration, freeze dehumidification concentration, hot air concentration, etc.; concentration temperature is 4~100 ° C, common temperature is best between 4~55 ° C; A low temperature vacuum concentration method is employed. Low-temperature vacuum concentration is characterized by high efficiency and high throughput, ensuring that the effective therapeutic ingredients in honey are not affected. The honey obtained after concentration was sampled and analyzed to have a water content of less than 20%.

After the concentrated dehydration and sterilization of honey, the samples are analyzed and analyzed to determine the content of the active ingredients to ensure the quality of the products. The active ingredients mainly refer to qiaosong, polysaccharides, and various phenolics. The phenolic resins, such as cichoric acid, chlorogenic acid, echinacoside, etc., are now internationally accepted analytical standards.

Method 2: Mixed method: Avenida honey can also be prepared by mixing Econesian extract concentrate with common honey. The Econesian extract concentrate is mixed with normal honey by a conventional method, and the ratio is generally between 1:1 and 100, and the usual ratio is between 1:1 and 10.

General honey refers to all honey known to humans, such as the following common jujube nectar, nectarine, nectar, pear nectar, sunflower nectar, buckwheat nectar, nectar nectar, lychee nectar, etc., but the present invention includes All bees

0 honey.

The honey used in the mixing may be honey before concentration and dehydration, or may be honey after concentration and dehydration. Generally, the newly harvested honey has a moisture content of more than 20%. It is easy to ferment and deteriorate after long-term storage, so it must be concentrated and dehydrated. The manner of concentration dehydration, the temperature range, and the like are as described above.

An appropriate amount of a surfactant and an antifoaming agent may be added to the mixing. Generally used pharmaceutically acceptable surfactants and antifoaming agents: 5 can be used in the present invention.

Both jossin and phenolic resins in Alcoa honey can be identified by physicochemical data and spectral analysis, such as high pressure liquid chromatography (HPLC) analysis, for qualitative and quantitative analysis.

Method for determining phenolic resin: The absorption peak area of various phenolic resins is uniformly quantified and added to the spectrum 5 001756 to calculate the total content;

The determination method of Qiaosong: The absorption peak on the spectrum should be clearly observed, and the peak height is at least three times that of the noise peak. For example, using a reversed-phase C-18, 5 Mm, 4. 6x250 匪 (Waters) column, the mobile phase is A: 30 亳mol of NaH2P04, B: acetonitrile; temperature 35oC, linear gradient method: 0-28 minutes: 10% B 30. /. B; 28-70 minutes, 30% B-^55% B; 70-75 minutes, 55% B->10% B, retention time of Qiaosongsu peak at 48.48 minutes, content of qiaosongsu reached 200 Yg/100g honey or more.

For Aikenian honey, the normal pH range is about 3. 4-5.6, so no additional acid is needed for stability and stability.

The Acenesian honey and edible adjuvant of the invention are prepared into an oral preparation. Such as soft capsules, oral liquids, tablets, pills, capsules, coated tablets or aerosols.

The liquid preparation such as an oral liquid may be a wine solution or an aqueous solution, or a suspension or a latex solution or the like. In the selection of solid formulations, it is desirable to use the corresponding excipients, and the choice of excipients will be readily apparent to those skilled in the art.

In the choice of oral liquid preparation, it is necessary to add appropriate flavoring agents, which may be honey, sorbose, xylose, xylitol, glycerin, disodium glycyrrhizinate, mannitol, mannose, galactose, maltose, lactose, Fructose, sweetener, sodium saccharin, stevioside, glucose, and sucrose.

Suitable preservatives and antioxidants may be added if necessary. Substances suitable as preservatives include Benzoic Acid, Sorbic Acid, Alcohol, Parabene, Benzene. Methanol (Benzyl Alcohol), and Phenethanolum, etc.; substances suitable as antioxidants include sodium hydrogen sulfite, sodium metabisulfite, sodium sulfite, dried sodium sulfite, sodium thiosulfate, ascorbic acid, methionine (methionine), Thiourea, disodium edetate (EDTA-Na2), phosphoric acid, citric acid, tert-butyl-p-hydroxyanisole (BHA), tert-butyl-p-cresol (BHT), nordihydrogen guaiac Acid (CDGA), tocopherol, phthalate esters, etc. Sometimes it is necessary to add an appropriate amount of aroma, a sizing agent, a coloring agent, etc. in the preparation. Fragrances include synthetic flavors such as esters, alcohols, aldehydes, ketones, oximes, etc., in different proportions, and cumin oil, rose oil, rose essence, lemon oil, lemon essence, vanilla essence, vanillin, Banana flavor, pineapple flavor, peppermint oil, orange peel oil, apple flavor, etc. The rubber paste is viscous and mild, and can interfere with the taste of the taste buds to achieve the purpose of flavoring. Commonly used are starch, gum arabic, scutellaria, carboxymethyl cellulose, methyl cellulose, sodium alginate, pectin. , agar, etc. Coloring agents such as synthetic caramel coloring and titanium dioxide. Others include the addition of emulsifiers and lipid or fat-soluble auxiliary substances which increase the degree of reabsorption of fat-soluble substances in the digestive system and increase their bioavailability, or the addition of water-diffusing auxiliary substances, or Two auxiliary substances are added at the same time, and so on. Commonly used lipid or fat-soluble auxiliary substances include tocopherol (tocopherol), vegetable oil, and lecithin (Lecithin). Commonly used water-soluble and water-diffusing auxiliary substances include lecithin, polysorbate 80, and oil lactic acid. Substances that can be used as emulsifiers include acacia, tragacanth, chalk, gelatin, shellac, (sea) sodium alginate, (anhydrous) lanolin, agar, cholesterol, lecithin, beeswax, petrolatum, whale Wax alcohol, stearyl alcohol, cetyl wax, paraffin wax, and the like.

An appropriate amount of a surfactant and an antifoaming agent are sometimes added to the oral liquid. Generally used pharmaceutically acceptable surfactants and antifoaming agents can be used in the present invention.

The Alcoholic honey of the present invention can be directly used, generally Aiconia honey (containing jossin, and the content of phenolic resin is not less than 0.5%), once a day in the morning and evening, one gram each time.

Orician oral preparations can be prepared in different specifications, such as size one: The content of phenolic resin in the oral preparation of Akonisia is 1.0%, then 5 ml per bottle, oral method, once in the morning and evening, each time A bottle; such as size 2: The content of phenolic resin in the Aikenian oral solution is 0.5%, then 10 ml per bottle, one time each morning and evening, one bottle at a time. Other concentrations of oral preparations can also be prepared, depending on the specific concentration of the phenolic resin, as determined by the amount of the oral preparation used herein.

Acenesian honey and Akonean oral preparations contain a certain amount of jossin and phenolic resin, making it more concentrated than Aikenian whole grass, Avenian juice or Aikenian extract, and ordinary honey Better medical results and health care features. DRAWINGS

Figure 1 is a HPLC chromatogram of the Aegean honey (GPS chromatogram at 330 nm);

Figure 2 is the LCQ MS fingerprint spectrum, which is the characteristic fingerprint spectrum of the Econicia honey;

Figure 3 is a graph showing changes in white blood cells in peripheral blood of rats;

Figure 4 is a graph comparing the number of lymphocytes (CD4+) in the blood surrounding the rat. Spectral analysis

In Fig. 1, rt=5. 2 minutes is caftaric acid, rt=8. 8 minutes is cholorgenic acid, rt=12. 6 minutes is cichoric acid, rt=14. 5 minutes is Qiao Songsu, rt=14. 8 Minutes is cichoric acid methyl ester.

The HPLC analysis method was compared with the standard sample of the Econion extract concentrate. The conditions of the commonly used HPLC analysis method were as follows: 20-minute linear gradient method using reverse phase Phenomenex Hydro-RP C18, 2. 0 X 150 mm solution The 1% formic acid, mobile phase B is 0. 1% formic acid, mobile phase B is 0.1% formic acid aqueous solution, mobile phase B is 0.1% formic acid, mobile phase B is 0. 1% formic acid Acetonitrile solution. The linear gradient method is: 0-10 minutes: 10°/oB^30%B; 10- 15 minutes, 30%B-90%B; 15- 15. 1 minute, 90%B-10%B; 15. 1 -20 minutes, 10% B.

Analytical detection means can be mass spectrometry (MS) and ultraviolet-visible (UV-Vis), as shown in Fig. 1 is a HPLC spectrum and UV-visible absorption detection using a wavelength of 330 nm.

The analytical method can also adopt the method of fingerprinting of the extract of the Econicus extract, such as electrospray ionization (ESI) mass spectrometry, as shown in Fig. 2 is the electrospray ionization of the extract of the Econicus extract. Mass spectrometry, using a positive ionization LCQ ion mass spectrometer (Ion-trap Mass Spectrometer), 50% aqueous acetonitrile as mobile phase, flow rate 0. 2 ml / min, mass scan range is m / z = 150 to m / z = between 2000. detailed description

The invention is further illustrated by the following specific examples. It is to be understood that the examples are not intended to limit the scope of the invention. The experimental methods in the following examples which do not specify the specific conditions are usually in accordance with conventional conditions or according to the conditions recommended by the manufacturer.

Example 1 Preparation method of Acenesia honey

Method 1 Harvest honey by beekeeping

(1) At the center of the Aikenian plantation, establish a beehive, using a wooden frame of 10 standard beehives, and check it regularly every .7 to 10 days. Check the items: Whether the queen bee exists, the queen bee lays eggs and the larvae Size, honey powder storage, pests and diseases, the weather should be selected when the weather is fine.

(2) In the no flowering period, artificial syrup feeding is needed to maintain the development of the bee colony. The syrup concentration is mainly sucrose, which is 45% sucrose, 10% Econesian extract concentrate and 45% water;

(3) When the honey in the nest is full, the honey collecting work can be carried out, and the sunny day is selected;

(4) The honey collected is analyzed after concentration in a vacuum at a low temperature, and the water content is less than 20%, pH 3. 4-5. 6 , the content of the ginseng is 200-2000 wg/100 g of honey, and the content of the phenolic resin is the total weight of the honey. 0. 5-3. 0%, then no Bottling under nitrogen protection, 50-100 ml per bottle o

Analyze and test the obtained Acenesian honey. Because each bee is different, the product has a certain difference, so the pH value is between 3.4 and 5.6. The detection method of qiaosong and phenolic resin is as described above. 5%。 0%. 0%. 0%. 0%. Method 2, general honey and Aikenian extract concentrate mixed

At 15 to 20 ° C, 10 liters of general honey concentrated under low temperature vacuum is slowly stirred under nitrogen protection, and 200 mg of medicinal methyl silicone oil 20 ppm and 50 g of medicinal polysorbate - 40 are added. After stirring evenly, 1.2 liters of the Econesian extract concentrate was slowly added under the protection of nitrogen, the mixture was continuously stirred at 20 ° C until the mixture was uniform, and then bottling under the protection of sterile nitrogen. , 50-100 ml per bottle o

Freshly harvested fresh honey can also be used directly to prepare Acenesian honey: In a low-temperature vacuum concentrator, 13 liters of freshly harvested fresh honey is slowly stirred under nitrogen and 200 mg of medicinal methyl is added. Silicone oil (20 _P pm concentration) and 50 g of medicinal polysorbate-40. After the honey can be easily stirred, 1.2 liters of Econesian extract concentrate (purchased from Affordable Supplements, USA, 4105) West Pawnee, Wichita, KS, USA) Slowly add under nitrogen protection, after low temperature (control temperature between 4~1Q °C), vacuum concentration, sample check, wait for water in Aikenian honey After less than 20%, the mixture was stirred at 20 ° C for one hour and then bottled under sterile nitrogen, such as 50-100 ml per bottle.

As in the first method, the obtained Econionia honey is analyzed and tested, and the devaluation value is between 3. 4 and 5. 6 and the content of the jossin is 200-200 (^g/100g honey, the content of the phenolic resin is the total weight) 0. 5-3. 0%. Example 2 Preparation method of Oriconic oral preparation

The gram of stimulating acid, 20 mg of medicinal methyl silicone oil (20 ppm concentration) and 5.0 g Medicinal Polysorbate - 40, to be mixed, add 500 ml of Avenida honey, 2. 0 g of disodium glycyrrhizinate, 12 g of gelatin, 10 g of glycerin, 12 g of lecithin And 50 grams of carboxymethyl cellulose. Under nitrogen, the mixture was stirred at 20 ^D C until it was well mixed, and then bottled under sterile nitrogen, 10 ml per bottle. Example 3 Animal test: method:

Animal experiments used three-month-old male Wistar rats, divided into six groups, eight in each group, 1-3 group A1, A2, A3 did not use X-ray whole body irradiation, 4- 6 group was Bl, B2 B3 uses X-ray whole body irradiation, respectively

1. Normal control group, Al.

2. The extract of the Econicus extract is fed into the abdominal cavity (the total weight of the phenolic resin is between 4. 0 and 4. 4%, TruNature product, 250 mg / kg body weight / day), A2.

3. The Acenesian honey prepared in Method 2 is fed into the abdominal cavity (the total weight of the phenolic resin is between 1. 0-1. 16%, 1000 mg / kg body weight / day), A3.

4. Use X-ray whole body irradiation, Bl.

5, using X-ray whole body irradiation, Acenesia extract concentrate is fed into the abdominal cavity (total weight of phenolic resin is between 4. 0-4. 4%, TruNature products, 250 mg / kg body weight / day) , B2.

6, using X-ray whole body irradiation, Method 2 prepared Acenesia honey is fed into the abdominal cavity (total weight of phenolic resin is between 1. 0-1. 16%, 1000 mg / kg body weight / day), B3 .

Result:

U) Using a (Phillips, Inc.) X-ray apparatus designed for animals, the rats were irradiated with a total of three Gy, and the irradiation speed was 0.35 Gy/min. Peripheral blood samples were drawn from the veins of the tail, and the leukocytes in the peripheral blood were measured using an automated blood cell counter (Coulter ACT 10), and data for 3 hours, 1 day, 3 days, 7 days, and 30 days were measured. '

In Figure 3: 1 on the abscissa is the base value before the test, and 2 to 6 are 3 hours, 1 day, 3 days, 7 days, and the '30 day value, respectively. The data in the figure is the average of the eight rat measurements.

The results of the test showed that the Econical extract concentrate and the Econician bee did not normally affect the white blood cells in the peripheral blood, but after the X-ray irradiation, the Econical extract was used. The number of white blood cells in the blood returned to near normal value at 30 days; when using Acenesian honey, the number of white blood cells in the blood returned to normal at 30 days. The Acenesia honey in the present invention is superior to the Aconnix; sub-extract concentrate. The results also suggest that Acenesian honey has a good adjuvant treatment and protection for patients receiving radiation therapy.

(2) The number of lymphocytes in the peripheral blood (CD4+) was measured after X-ray irradiation in rats, and a conventional standard separation and measurement method was employed. The data in Figure 4 is the average of the eight rat measurements.

The results of the test showed that the Econesian extract concentrate increased the number of lymphocytes in the peripheral blood, while the Acenesian honey acted slightly better than the Akonian extract concentrate. Both of them have a certain protective effect on lymphocytes in the peripheral blood when exposed to X-rays, and the Acenesia honey in the present invention is superior to the extract of Econionia extract. The results also suggest that pre-administration of Acenesian honey prior to receiving radiation therapy can reduce the damaging effects of radiation therapy on the patient's immune system.

Claims

Rights request

1. An Avenida honey characterized by having an active ingredient of Avenida and honey.

2, an Aiconia honey, characterized in that the Aikenian honey contains jossin and phenolic resin, wherein the content of jossin is 200-2000 μg / 100g honey, and the content of phenolic resin is 0. 5-3. 0%.

3. An oral preparation of Acenesia honey, characterized in that the oral preparation consists of Acenesian honey and edible excipients, which contains 200-2000 yg/100 g of honey, and the content of phenolic resin is the total weight of honey. 0. 5-3. 0%.

4. An oral preparation of Acenesia honey according to claim 3, characterized in that the oral preparation is an oral solution, a tablet, a pill, a capsule, a soft capsule, a coated tablet, an aerosol or other liquid preparation.

5. An oral preparation of Acenesia honey according to claim 3, characterized in that the oral preparation is a wine solution, an aqueous solution, a suspension or a latex.

6. A method of preparing Acenesian honey, characterized in that the method comprises the following steps:

(1) In the center of the Aixinian plantation, establish a beehive, using wooden 10 frame standard beehives, every 7~

Regular inspection once every 10 days, check the project: Whether the queen bee exists, the queen bee lays eggs and the size of the larvae, the storage of the honey powder, the occurrence of pests and diseases, the weather should be selected when the weather is fine;

(2) In the no-flowering period, artificial syrup feeding is needed to maintain the development of the bee colony. The syrup concentration is 45% sucrose, 10% Econesian extract concentrate and 45% water V/V;

0 (3) When the honey in the nest is full, the honey collecting work can be carried out, and the sunny day is selected;

(4) The collected honey is analyzed after concentration, the water content is less than 20%, pH 3. 4-5. 6 , the content of jossin is 200-2000 g/100g honey, and the content of phenolic resin is 0. 5-3. 0%, then bottled under sterile nitrogen. '

7. The method for preparing a honey of Acenesia according to claim 6, wherein the step: 5 (4) is concentrated by low temperature vacuum concentration, infrared concentration, freeze dehumidification or hot air. Concentrated; preferably concentrated under low temperature vacuum; the concentration temperature is 4-100 Torr, and the preferred temperature is 4-25 Torr.

8. A method for preparing a honey of Acenesia, characterized in that the method comprises: slowly stirring a general honey concentrated under a low temperature vacuum at 15 to 200 C under a nitrogen atmosphere, and adding a medicinal methyl group. 20ppm concentration of silicone oil and medicinal polysorbate-40, after evenly stirring, the Econesian extract concentrate is slowly added under the protection of nitrogen, the mixture is continuously stirred at 200C until the mixture is homogenized, and then in the sterilized Bottling under nitrogen protection.

9. The method of preparing Alcoholic honey according to claim 8, wherein the mixing ratio of the extract of the Enicia extract to the general honey is 1: 1 to 100, preferably 1 : 1 to 10.

The method for preparing a honey of Acenesia according to claim 8, wherein the general honey is jujube nectar, nectarine, nectar, pear nectar, sunflower nectar, buckwheat nectar, and nectar nectar. Or lychee nectar.