WO2006124456A3 - Competitive particle immunoassay methods utilizing fluorescence microscopy - Google Patents

Competitive particle immunoassay methods utilizing fluorescence microscopy Download PDF

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Publication number
WO2006124456A3
WO2006124456A3 PCT/US2006/018085 US2006018085W WO2006124456A3 WO 2006124456 A3 WO2006124456 A3 WO 2006124456A3 US 2006018085 W US2006018085 W US 2006018085W WO 2006124456 A3 WO2006124456 A3 WO 2006124456A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence microscopy
methods utilizing
immunoassay methods
competitive
utilizing fluorescence
Prior art date
Application number
PCT/US2006/018085
Other languages
French (fr)
Other versions
WO2006124456A2 (en
Inventor
Julius Tyczkowski
Dipak Mahato
Alan Chalker
Original Assignee
Embrex Inc
Julius Tyczkowski
Dipak Mahato
Alan Chalker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Embrex Inc, Julius Tyczkowski, Dipak Mahato, Alan Chalker filed Critical Embrex Inc
Priority to CA002607284A priority Critical patent/CA2607284A1/en
Priority to JP2008511320A priority patent/JP2008541090A/en
Priority to EP06759493A priority patent/EP1880207A4/en
Priority to MX2007014096A priority patent/MX2007014096A/en
Priority to BRPI0608805-8A priority patent/BRPI0608805A2/en
Priority to AU2006247747A priority patent/AU2006247747A1/en
Publication of WO2006124456A2 publication Critical patent/WO2006124456A2/en
Publication of WO2006124456A3 publication Critical patent/WO2006124456A3/en
Priority to IL187051A priority patent/IL187051A0/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
    • A01K45/007—Injecting or otherwise treating hatching eggs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

Abstract

The present invention provides methods of measuring analytes in a sample by competitive imunoassay utilizing flurescence microscopy In particular embodiments, the invention provides a method of determining the gender of an avian embryo in ovo by determining the presence of an estrogenic steroid compound m a sample of embryonic fluid (e g , allantoic fluid or blood) from the avian egg (Fig 1).
PCT/US2006/018085 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy WO2006124456A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002607284A CA2607284A1 (en) 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy
JP2008511320A JP2008541090A (en) 2005-05-11 2006-05-10 Competitive microparticle immunoassay using fluorescence microscopy
EP06759493A EP1880207A4 (en) 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy
MX2007014096A MX2007014096A (en) 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy.
BRPI0608805-8A BRPI0608805A2 (en) 2005-05-11 2006-05-10 methods for detecting the presence of an analyte in a sample and for determining the sex of a bird embryo in an egg
AU2006247747A AU2006247747A1 (en) 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy
IL187051A IL187051A0 (en) 2005-05-11 2007-10-30 Competitive particle immunoassay methods utilizing fluorescent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67971705P 2005-05-11 2005-05-11
US60/679,717 2005-05-11

Publications (2)

Publication Number Publication Date
WO2006124456A2 WO2006124456A2 (en) 2006-11-23
WO2006124456A3 true WO2006124456A3 (en) 2007-08-09

Family

ID=37431849

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/018085 WO2006124456A2 (en) 2005-05-11 2006-05-10 Competitive particle immunoassay methods utilizing fluorescence microscopy

Country Status (10)

Country Link
EP (1) EP1880207A4 (en)
JP (1) JP2008541090A (en)
KR (1) KR20080015844A (en)
CN (1) CN101218506A (en)
AU (1) AU2006247747A1 (en)
BR (1) BRPI0608805A2 (en)
CA (1) CA2607284A1 (en)
IL (1) IL187051A0 (en)
MX (1) MX2007014096A (en)
WO (1) WO2006124456A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5564491B2 (en) * 2008-04-29 2014-07-30 サイケメディクス コーポレイション Solid phase multi-analyte assay
JP5419012B2 (en) * 2010-01-12 2014-02-19 独立行政法人理化学研究所 Manufacturing method and manufacturing apparatus of substrate on which probe is fixed
WO2013025532A2 (en) * 2011-08-12 2013-02-21 Siemens Healthcare Diagnostics Inc. Detection of sex steroids
WO2014021715A2 (en) 2012-07-30 2014-02-06 Bruins Wouter Sebastiaan Gender, viability and/or developmental stage determination of avian embryos in ovo
US10032270B2 (en) 2013-03-15 2018-07-24 Richard H. Turner System and methods for the in vitro detection of particles and soluble chemical entities in body fluids
JP2017524899A (en) * 2014-05-22 2017-08-31 ゾエティス・サービシーズ・エルエルシー Method and apparatus for selectively processing eggs according to gender and other characteristics
DE102015226490A1 (en) * 2015-12-22 2017-06-22 Universität Leipzig Procedure for in ovo gender determination of chicks
WO2017204636A2 (en) 2016-05-24 2017-11-30 In Ovo B.V. Method and system for the non-destructive in ovo determination of fowl gender
CR20190569A (en) 2017-05-25 2020-04-19 Eggxyt Ltd Methods for gender determination of avian embryos in unhatched eggs and means thereof
WO2020075104A1 (en) * 2018-10-10 2020-04-16 Bord Bar Gholamreza Chick embryo candling via injection of fluorescent and phosphorescence to the male bird
NL2023176B1 (en) 2019-05-22 2020-12-01 In Ovo B V Composition comprising eggs and use of such composition

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365339B1 (en) * 1998-09-04 2002-04-02 Bechtel Bwxt Idaho, Llc Gender determination of avian embryo
US20040106215A1 (en) * 2000-10-27 2004-06-03 Werner Lehmann Method and test kit for detecting analytes in a sample

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6435373A (en) * 1987-07-31 1989-02-06 Fujirebio Kk Method and device for high-sensitivity immunoassay
US5377618A (en) * 1992-07-31 1995-01-03 Reproductive Sciences, Inc. Method for preferential production of ratites of a desired sex
JPH0829422A (en) * 1994-07-12 1996-02-02 Aisin Seiki Co Ltd Detecting method by using fluorescent aggregate
BRPI9712485B1 (en) * 1996-10-04 2017-05-30 Embrex Inc Soc Norte Americana Method of classifying birds in ovo
US7041439B2 (en) * 2001-04-17 2006-05-09 Embrex, Inc. Methods and apparatus for selectively processing eggs having identified characteristics
CA2445162C (en) * 2001-04-23 2011-04-05 Lifesensors, Inc. Estrogen dependent transactivation system for gender sorting in avian species
JP2004028682A (en) * 2002-06-24 2004-01-29 Juki Corp Measuring method of object substance
JP2004150997A (en) * 2002-10-31 2004-05-27 Kyogo Ito Method of measureing cytokine
JP4257513B2 (en) * 2003-09-12 2009-04-22 学校法人早稲田大学 Biosensing method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365339B1 (en) * 1998-09-04 2002-04-02 Bechtel Bwxt Idaho, Llc Gender determination of avian embryo
US20040106215A1 (en) * 2000-10-27 2004-06-03 Werner Lehmann Method and test kit for detecting analytes in a sample

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HANNINEN ET AL.: "A new microvolume technique for bioaffinity assay using two-photon excitation", NATURE BIOTECHNOLOGY, vol. 18, May 2000 (2000-05-01), pages 548 - 550, XP002957962 *
SOINI ET AL.: "Two-photon fluorescence excitation in detection of biomolecules", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 28, no. PART 2, 2000, pages 70 - 75, XP003016738 *
WIKLUND ET AL.: "Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay", ANALYTICAL BIOCHEMISTRY, vol. 338, 2005, pages 90 - 101, XP004747520 *

Also Published As

Publication number Publication date
EP1880207A2 (en) 2008-01-23
AU2006247747A1 (en) 2006-11-23
MX2007014096A (en) 2008-02-07
IL187051A0 (en) 2008-02-09
CN101218506A (en) 2008-07-09
BRPI0608805A2 (en) 2010-11-30
JP2008541090A (en) 2008-11-20
EP1880207A4 (en) 2010-09-08
KR20080015844A (en) 2008-02-20
WO2006124456A2 (en) 2006-11-23
CA2607284A1 (en) 2006-11-23

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