WO2006118595A2 - Nanostructured thin films and their uses - Google Patents
Nanostructured thin films and their uses Download PDFInfo
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- WO2006118595A2 WO2006118595A2 PCT/US2005/032962 US2005032962W WO2006118595A2 WO 2006118595 A2 WO2006118595 A2 WO 2006118595A2 US 2005032962 W US2005032962 W US 2005032962W WO 2006118595 A2 WO2006118595 A2 WO 2006118595A2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/30—Inorganic materials
- A61L27/303—Carbon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/12—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L31/121—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
- A61L31/122—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of carbon
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0409—Sample holders or containers
- H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/70—Nanostructure
- Y10S977/701—Integrated with dissimilar structures on a common substrate
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/12—All metal or with adjacent metals
- Y10T428/12493—Composite; i.e., plural, adjacent, spatially distinct metal components [e.g., layers, joint, etc.]
- Y10T428/12736—Al-base component
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/12—All metal or with adjacent metals
- Y10T428/12493—Composite; i.e., plural, adjacent, spatially distinct metal components [e.g., layers, joint, etc.]
- Y10T428/12736—Al-base component
- Y10T428/12743—Next to refractory [Group IVB, VB, or VIB] metal-base component
Definitions
- the present invention relates primarily to the field of nanotechnology. More specifically, the invention pertains to nanostructured thin films and coatings and their uses in, e.g., high surface area applications such as mass spectrometry, as super-hydrophobic coatings, as anti-bifouling coatings, as surfaces to promote cell attachment, differentiation and proliferation, etc.
- Nanotechnology has been heralded as the next major technological leap, in that it is prophesied to yield a variety of substantial advantages in terms of material characteristics, including electronic, optical and structural characteristics.
- Nanostructured materials as thin films and coatings possess unique properties due to both size and interface effects.
- Nanostructured materials are generally a broad class of materials, with microstructures modulated in zero to three dimensions on length scales typically less than about 500 nm, for example, less than about 200 nm, e.g., less than about 100 nm.
- Nanostructured materials find many applications in areas such as electronics, mass spectrometry, catalysis, protection, data storage, optics, and sensors.
- Nanostructured films and coatings have many advantages over conventional thin films including high surface area, increased hydrophobicity, increased adhesion, and other similar properties.
- Electrospray ionization ESI
- MALDI matrix-assisted desorption/ionization
- ESI allows for large, non-volatile molecules to be analyzed directly from the liquid phase.
- MALDI ionizes a sample by pulsed laser irradiation of the sample.
- the sample is co-crystallized with a solid matrix that can absorb the wavelength of light emitted by the laser.
- MALDI matrix-assisted laser desorption/ionization
- Silicon nanowires appear to be an ideal platform for surface-based mass spectrometry. In contrast to porous silicon, silicon nanowires are catalyzed and grown on the surface of a substrate and their physical dimensions, composition, density, and position can be precisely controlled at the nanoscale level, thus offering even greater potential for designing mass spectrometry active surfaces.
- the present invention provides unique nanostructured thin film surfaces to generate high surface area substrates for matrix-free MALDI and other applications as well. By eliminating background peaks of interfering matrix compounds, good analyses of both low and high-molecular weight compounds such as small molecules, proteins, peptides, oligonucleotides, drugs, pesticides, carbohydrates, fatty acids and the like can be produced more quickly and reliably.
- the present invention generally discloses the use of a nanostructured non- silicon thin film (such as an alumina or aluminum thin film) on a supporting substrate which is subsequently coated with an active layer of a second material (e.g., silicon, tungsten, etc.).
- a nanostructured non-silicon thin film such as an alumina or aluminum thin film
- the base underlying non-silicon material generates enhanced surface area while the active layer assists in incorporating and transferring energy to one or more analytes adsorbed on the active layer when irradiated with a laser during laser desorption of the analyte(s).
- the present invention provides substrate surfaces that can be produced by relatively straightforward and inexpensive manufacturing processes.
- the nanopatterned thin film produced by the methods of the present invention has characteristics of high uniformity over a relatively large area, strong adhesion to the underlying substrate, resistance to scratching, and can be easily applied onto large substrate materials.
- the nanostructured film may be used as a base layer for increasing the surface area of a substrate, e.g., for matrix-free mass spectrometry applications, for making a substrate surface superhydrophilic or superhydrophobic, for modifying the optical properties of a substrate, and/or for developing highly efficient catalytic surfaces, e.g., for the growth of nanofibers or other nanostructured components thereon.
- a device which generally comprises a supporting substrate, a first layer of a nanostructured coating, and a second layer of an active coating.
- the supporting substrate can take a variety of forms such as a semiconductor wafer, a sheet of glass or quartz, a sheet of metal, a piece of ceramic or plastic.
- the first layer of nanostructured coating can comprise, for example, elongated or plate-like grains with at least one dimension less than about 0.2 ⁇ m. The long axis of plate- like grains may be oriented substantially perpendicular to a surface of the supporting substrate.
- the first layer of nanostructured coating comprises an insulating inorganic material such as a native oxide layer or a deposited oxide or nitride layer.
- the insulating inorganic material may also be formed form a deposited metal layer.
- the insulating inorganic material may be selected from the group of materials including alumina (Al 2 O 3 ), aluminum (Al), SiO 2 , ZrO 2 , HfO 2 , a hydrous form of these oxides, a compound oxide such as SiTiO 3 , BaTiO 3 PbZrO 4 or a silicate.
- the active coating layer comprises a semiconductor which absorbs light energy such as IR, red, green, blue and/or UV laser light energy.
- the semiconductor may be selected from Group IV semiconductors including Si, Ge, diamonds and diamond-like carbon coatings, or compound semiconductors of Group H-V, II- VI and other semiconductors including sulfides, selenides, tellurides, nitrides, carbides, nitrides, antimonides, and phosphides.
- the active layer may also comprise a metal such as tungsten or other metal which is catalytically active and interferes with light absorption, and which is capable of promoting or enhancing light energy transfer to an analyte, e.g., to ionize and desorb it from the surface of the supporting substrate.
- the metal may be selected from the group comprising transition metals including Fe, Co, Cr, Ni, Mo, W, V, Cu, Zn and precise metals including Ag, Au, Pt, Pd, Ru, Rh, or metal alloys thereof.
- a mass spectrometry device which comprises a supporting substrate having a first surface and a thin non-silicon film layer of a first material deposited on at least a region of the first surface, the film layer having a nanostructured surface; an active layer of a second material deposited on the first layer; and at least a first analyte positioned in contact with at least a region of the active layer, wherein the active layer assists in incorporating and transferring energy to the at least first analyte when irradiated with a laser during laser desorption of the analyte.
- the non-silicon film layer can be deposited on the substrate using a variety of well-known techniques such as thermal evaporation and sputtering including physical vapor deposition (PVD), sputter deposition, low or high temperature chemical vapor deposition (CVD), metallorganic CVD, plasma-enhanced CVD, laser ablation, or using solution deposition methods such as spray coating, dip coating, or spin coating etc.
- Ultra-thin metal films e.g., films less than about 5nm in thickness
- ALD atomic layer deposition
- the thin film may be configured to have a nanostructured surface by, for example, simply exposing the film to hot water or water vapor.
- the thin film surface is exposed to hot water or water vapor at a temperature greater than about 50 degrees Celsius, e.g., between about 50 to 150 degrees Celsius.
- the water or the water vapor preferably has a temperature of from about 90 to 125 degrees Celsius.
- the film surface is exposed to the water or water vapor for a sufficient time (e.g., between about 3 to 60 minutes, for example, between about 5 and 30 minutes) to convert the film into a highly ordered nanostructured surface.
- the thin film is a metal thin film made from alumina or aluminum.
- textured surfaces can also be used as long as such surfaces enhance desorption sensitivity, including, for example, a native oxide layer or a deposited oxide or nitride layer or materials including ZnO, SiO 2 , ZrO 2 , HfO 2 , a hydrous form of these oxides, a compound oxide such as SiTiO 3 , BaTiO 3 PbZrO 4 or a silicate.
- the supporting substrate itself is made from a non-silicon material such as alumina or aluminum from which a nanostructured surface can be formed, and the active layer is deposited on the nanostructured surface of the substrate.
- the active layer may comprise a semiconductor material such as silicon or germanium, or other conducting or semiconducting materials, such as metals and semimetals, and other materials which absorb light and promote or enhance light energy transfer to an analyte to ionize and desorb it from the surface of the supporting substrate as described above and further below.
- the active layer is made from silicon, and the silicon active layer is deposited on the thin film layer by a chemical vapor deposition process for ten or more minutes to a depth of about 50 nm or more, or by other thermal evaporation method such as sputtering.
- the active layer can also optionally be coated or functionalized, e.g., to enhance or add specific properties.
- polymers, ceramics, or small molecules can optionally be used as coating materials.
- the optional coatings can impart characteristics such as water resistance, improved mechanical, optical (e.g., enhancement of light coupling) or electrical properties or specificities for certain analytes.
- the active layer may also be derivatized with one or more functional moieties to enhance laser desorption such as one or more silane groups, e.g., one or more per-fluorinated silane groups, or other coatings such as diamond-like carbon thin film coatings (e.g., to render the surface of the film hydrophobic).
- the supporting substrate may be made from any one of a number of conventional materials including stainless steel, glass, quartz, semiconductor materials such as silicon, polymers, ceramics etc.
- Samples of the substances (e.g., small molecules, proteins, peptides etc.) to be analyzed are optionally placed in contact with the active layer (e.g., directly or via the use or one or more functional moieties such as one or more silane groups) by conventional dispensing means such as pipetting, dot-printing etc.
- the active layer e.g., directly or via the use or one or more functional moieties such as one or more silane groups
- Laser energy levels and pulse durations are also optionally optimized for analysis of the samples arrayed upon the nanostructured substrate surface.
- those of skill in the art will be familiar with ways of determining optimal parameters for laser energy, pulse time, etc. for mass spectrometry.
- the different parameters are optionally modified depending upon, e.g., the specific molecules being detected.
- a mass spectrometry system which generally comprises a substrate comprising a first surface and a thin non- silicon film layer of a first material (e.g., a metal such as alumina or aluminum) on at least a region of the first surface, the film having a nanostructured surface; an active layer of a second material deposited on the first layer; at least a first analyte positioned in contact with a first region of the active layer; a laser positioned to direct energy at the at least first region to desorb the at least first analyte from the first region; and a mass spectrometer instrument positioned to receive the at least first analyte desorbed from the active layer, wherein the active layer assists in incorporating and transferring energy to the at least first analyte during laser desorption.
- a first material e.g., a metal such as alumina or aluminum
- the active layer may be functionalized or coated with one or more functional moieties to enhance or add specific properties, e.g., to render its surface hydrophobic.
- methods are provided for manufacturing substrates for various applications including mass spectrometry, as well as methods for detecting one or more analytes, e.g., for mass spectrometry analysis, using such substrates.
- a method for manufacturing a substrate device which comprises providing a supporting substrate having a first surface; depositing a non- silicon film layer on the first surface; forming a nanostructured surface from the non-silicon film layer; depositing an active layer on the nanostructured surface; and depositing one or more analytes onto one or more regions of the active layer.
- the step of depositing a non-silicon film layer on the first surface of the substrate can comprise, for example, sputtering the film onto the first surface or using a CVD method to deposit the film on the surface of the substrate.
- the non-silicon film can comprise, for example, an alumina or aluminum film from which a nanostructured film surface may be easily formed, or other similar nanostructured surface.
- the step of forming the nanostructured surface from the film layer can comprise, for example, exposing the film layer to hot water or water vapor at a temperature of greater than about 50 degrees Celsius, e.g., between about 50 to 150 degrees Celsius, for example, between about 90 to 125 degrees Celsius, for between about three to sixty minutes, to form a highly structured surface.
- the step of depositing the active layer on the non-silicon film layer can comprise, for example, using a chemical vapor deposition process to deposit such film, wherein such film comprises a silicon or tungsten film in exemplary embodiments.
- a chemical vapor deposition process to deposit such film, wherein such film comprises a silicon or tungsten film in exemplary embodiments.
- substrates useful for a variety of applications such as mass spectrometry can be reproducibly produced easily and with relatively little cost, e.g., as compared to wet electrochemically etched porous silicon films.
- the method may further comprise derivatizing the active layer with one or more functional moieties to, e.g., increase the hydrophobicity of the surface and/or to assist in desorbing the one or more analytes from the surface of the active layer.
- a method for analyzing one or more analytes which generally comprises providing a supporting substrate having a first surface and a thin non-silicon film layer deposited on the first surface and an active " layer deposited on the thin film layer, the thin film layer having a nanostructured surface; and analyzing one or more analytes associated with the active layer by one or more detection means such as mass spectrometry analysis.
- a method for performing mass spectrometry comprises providing a substrate having a first surface and a thin film layer (e.g., a non- silicon layer such as alumina or aluminum) deposited on the first surface and an active layer deposited on the thin film layer, the thin film layer having a nanostructured surface and one or more analytes associated with the surface; desorbing the one or more analytes from the surface with energy from a laser directed at the surface; and analyzing the one or more analytes in a mass spectrometer instrument.
- the analytes to be measured include, for example, proteins, peptides, carbohydrates, fatty acids, small molecules, nucleic acids, cells, polypeptides and mixtures thereof.
- the analytes may be directly applied to the surface of the substrate in solid, dried form or in liquid form and dried on the substrate.
- the analytes may be suspended in aqueous or organic solutions or suspensions.
- a mass spectrometry device which comprises a supporting substrate having a first surface and a plurality of nanocrystals, e.g., semiconductor nanocrystals such as quantum dots, deposited on the surface of the substrate.
- nanocrystals e.g., semiconductor nanocrystals such as quantum dots
- the nanocrystals can be a gold nanoparticle, a cobalt nanoparticle, an iron oxide nanoparticle, or a semiconductor nanocrystal including a semiconductor material, such as a Group IV compound (e.g., Si, Ge), a Group II- VI compound, a Group H-V compound, a Group III- VI compound, a Group III-V compound, a Group IV-VI compound, a Group I-III-VI compound, a Group II-IV-VI compound, or a Group II-IV-V compound.
- a Group IV compound e.g., Si, Ge
- a Group II- VI compound e.g., Si, Ge
- a Group II- VI compound e.g., a Group H-V compound
- a Group III- VI compound e.g., a Group III-V compound
- a Group IV-VI compound e.g., a Group I-III-VI compound, a Group II-IV-VI compound, or a Group II-IV-
- the semiconductor material can be, for example, ZnS, ZnSe, ZnTe, CdS, CdSe, CdTe, HgS, HgSe, HgTe, AlN, AIP, AlAs, AlSb, GaN, GaP, GaAs, GaSb, GaSe, InN, InP, InAs, InSb, TIN, TIP, TIAs, TlSb, PbS, PbSe, PbTe, or mixtures thereof.
- the nanoparticle can be coated with one or more second materials such as a second metal, semimetal, polymer, or semiconductor material such as ZnO, ZnS, ZnSe, ZnTe, CdO, CdS, CdSe, CdTe, MgO, MgS, MgSe, MgTe, HgO, HgS, HgSe, HgTe, AIN, AIP, AlAs, AlSb, GaN, GaP, GaAs, GaSb, InN, InP, InAs, InSb, TIN, TIP, TIAs, TlSb, TlSb, PbS, PbSe, PbTe, or mixtures thereof.
- a second metal, semimetal, polymer, or semiconductor material such as ZnO, ZnS, ZnSe, ZnTe, CdO, CdS, CdSe, CdTe, MgO, MgS, MgSe, M
- a device for enhanced surface area and/or hydrophobic surface applications other than mass spectrometry (e.g., such as for use in medical device applications, building materials, barrier layers for storage tanks, a surface for cell attachment, differentiation and proliferation, contact touch screens for use with ATM machines, in car displays, in entertainment portals, etc.) is disclosed which generally comprises a supporting substrate having a surface, a non-silicon film layer on at least a region of the surface, the film layer having a nanostructured surface, and a hydrophobic (or hydrophilic) coating deposited on the film layer.
- the hydrophobic coating may comprise, for example, a diamond-like carbon coating or other coating which renders the surface of the substrate hydrophobic (or hydrophilic).
- the diamond-like carbon film can be applied to the nanostructured surface by, e.g., using pulsed laser ablation on a graphite target or a plasma assisted CVD method at low temperature. When the deposited material is heated, such films lose their stress, yet retain their diamond-like properties.
- Figure 1 shows a configuration of an exemplary laser desorption/ionization mass spectrometry setup.
- Figure 2A is a cross-sectional view of a mass spectrometry substrate according to the teachings of the present invention.
- Figure 2B is an illustration of the substrate of Figure 2A derivatized with functionalities by silylation.
- Figure 3 shows an SEM close-up image of a nanostructured alumina film on a stainless steel substrate.
- Figure 4A shows an electron micrograph of a substrate having a nanostructured alumina surface with an active silicon layer deposited thereon.
- Figure 4B is a cross-sectional electron micrograph view of the substrate surface of Figure 4 A.
- Figure 5A shows results of mass spectrometry analysis of samples on a stainless steel substrate without any nanostructured thin film layer.
- Figure 5B shows results of mass spectrometry analysis of samples on a stainless steel substrate with a silicon layer and without any underlying nanostructured thin film layer.
- Figure 5C shows results of mass spectrometry analysis of samples on a stainless steel substrate with a thin nanostructured film layer and a silicon active layer according to the teachings of the present invention
- Figure 6A shows results of mass spectrometry analysis of 25 pg of clonidine deposited on a silicon coated nanostructured alumina surface.
- Figure 6B shows results of mass spectrometry analysis of 25 pg of haloperidol deposited on a silicon coated nanostructured alumina surface.
- Figure 6C shows results of mass spectrometry analysis of 25 pg of prazosin deposited on a silicon coated nanostructured alumina surface.
- Figure 6D shows results of mass spectrometry analysis of 25 pg of quinidine deposited on a silicon coated nanostructured alumina surface.
- Figure 7A shows results of mass spectrometry analysis of 2.5 pg of clonidine deposited on a silicon coated nanostructured alumina surface.
- Figure 7B shows results of mass spectrometry analysis of 2.5 pg of haloperidol deposited on a silicon coated nanostructured alumina surface.
- Figure 7C shows results of mass spectrometry analysis of 2.5 pg of prazosin deposited on a silicon coated nanostructured alumina surface.
- Figure 7D shows results of mass spectrometry analysis of 2.5 pg of quinidine deposited on a silicon coated nanostructured alumina surface.
- Figure 8A shows results of mass spectrometry analysis of 25 pg of clonidine deposited on a silicon coated nanostructured aluminum substrate surface.
- Figure 8B shows results of mass spectrometry analysis of 25 pg of haloperidol deposited on a silicon coated nanostructured aluminum substrate surface.
- Figure 8C shows results of mass spectrometry analysis of 25 pg of prazosin deposited on a silicon coated nanostructured aluminum substrate surface.
- Figure 8D shows results of mass spectrometry analysis of 25 pg of quinidine deposited on a silicon coated nanostructured aluminum substrate surface.
- Figure 9A shows results of mass spectrometry analysis of 25 pg of haloperidol deposited on a silicon coated nanostructured alumina surface that had the silicon active layer deposited by CVD for a deposition time of about 30 minutes.
- Figure 9B shows results of mass spectrometry analysis of 25 pg of haloperidol deposited on a silicon coated nanostructured alumina surface that had the silicon active layer deposited by CVD for a deposition time of about 10 minutes.
- Figure 1OA shows results of mass spectrometry analysis of 25 pg of prazosin deposited on a tungsten coated nanostructured aluminum substrate surface.
- Figure 1OB shows results of mass spectrometry analysis of 25 pg of haloperidol deposited on a tungsten coated nanostructured aluminum substrate surface.
- Figure 1OC shows results of mass spectrometry analysis of 25 pg of quimdine deposited on a tungsten coated nanostructured aluminum substrate surface.
- Figure 1OD shows results of mass spectrometry analysis of 25 pg of clonidine deposited on a tungsten coated nanostructured aluminum substrate surface.
- the present invention describes apparatus and methods of use of a nanostructured non-silicon thin film (such as an alumina or aluminum thin film) on a supporting substrate which is subsequently coated with an active material layer.
- the base non- silicon material generates enhanced surface area and the appropriate structural dimensions while the active layer assists in incorporating and transferring energy to one or more analytes adsorbed on the active layer when irradiated with a laser during laser desorption of the analyte(s).
- Figure 1 depicts a schematic configuration of an exemplary laser desorption/ionization mass spectrometry set-up showing a MALDI plate 8 customarily used in MALDI studies holding modified supporting substrate 10 illuminated by a series of laser pulses 14.
- the supporting substrate 10 can have a plurality of analytes 12 (e.g., proteins, peptides, small molecules etc.) adsorbed to it, preferably without the use of a matrix material used in conventional MALDI analyses.
- analytes 12 e.g., proteins, peptides, small molecules etc.
- the modified surface of the substrate absorbs the laser pulses 14 and ionizes and releases the analytes to form a desorbed and ionized analytes 13.
- the desorbed and ionized analytes 13 then travel to a mass spectrometry instrument (not shown) for analysis.
- FIG. 2A shows an enlarged cross-sectional view of the modified supporting substrate 10 according to the teachings of the present invention.
- the supporting substrate can take a variety of forms such as a semiconductor wafer, a sheet of glass or quartz, a sheet of metal, a piece of ceramic or plastic, and the like.
- the supporting substrate 10 has a first surface 20 and a thin non-silicon film layer 22 of a first material deposited on at least a region of the first surface, the film layer having a nanostructured surface.
- An active layer 24 of a second material is deposited on the first layer.
- a plurality of analytes 12 are positioned in contact with at least a region of the active layer 22, either directly or via the use of one or more functional groups as shown in Figure 2B.
- the active layer assists in incorporating and transferring energy (e.g., light energy from the laser pulses) to the analytes when irradiated with a laser during laser desorption of the analyte.
- energy e.g., light energy from the laser pulses
- the analyte molecules are ionized by acid-base proton transfer reactions with the protonated active layer ions in a dense phase just above the surface of the active layer.
- the protonated active layer molecules are generated by a series of photochemical reactions.
- the first non-silicon layer may comprise an insulating inorganic material such as a native oxide layer or a deposited oxide or nitride layer.
- the insulating inorganic material may also be formed form a deposited metal layer.
- the insulating inorganic material may be selected from the group of materials including aluminum (Al), alumina (Al 2 O 3 ), ZnO, SiO 2 , ZrO 2 , HfO 2 , a hydrous form of these oxides, a compound oxide such as SiTiO 3 , BaTiO 3 PbZrO 4 or a silicate.
- the non-silicon film layer is made from alumina or aluminum which can be deposited on the substrate using a variety of well-known techniques such as thermal evaporation and sputtering including physical vapor deposition (PVD), sputter deposition, chemical vapor deposition (CVD), metallorganic CVD, plasma-enhanced CVD, laser ablation, or solution deposition methods such as spray coating, dip coating, or spin coating etc.
- PVD physical vapor deposition
- CVD chemical vapor deposition
- metallorganic CVD metallorganic CVD
- plasma-enhanced CVD plasma-enhanced CVD
- laser ablation or solution deposition methods
- solution deposition methods such as spray coating, dip coating, or spin coating etc.
- Ultra-thin metal films e.g., films less than about 5nm in thickness
- ALD atomic layer deposition
- the thin film preferably has a thickness less than about 1000 nm, for example, between about 5 and 400 nm, for example, between about 5 and 200 nm, for example, between about 10 and 100 nm.
- the thickness of the substrate may be significantly greater, e.g., on the order of about 0.5 mm or thicker.
- the film layer may be configured to have a nanostructured surface as shown, for example, in the SEM images of Figure 3 and 4, by, for example, simply exposing the film to hot water or water vapor.
- the surface is exposed to hot water or water vapor at a temperature greater than about 50 degrees Celsius, e.g., between about 50 to 150 degrees Celsius.
- the water or the water vapor preferably has a temperature of from about 90 to 125 degrees Celsius.
- FIGS. 4A-B are electron micrographs of a nanostructured alumina surface with an active silicon layer deposited thereon.
- the nanostructured alumina layer comprises elongated or plate-like grains with at least one dimension less than about 0.2 ⁇ m. The long axis of the plate-like grains are oriented substantially perpendicular to the surface of the supporting substrate.
- the nanostructured alumina layer can also be formed by other means such as the formation of porous alumina films via the anodization of aluminum metal in acidic solution (e.g., phosphoric, oxalic, or sulfuric acid solutions), autoclaving the film layer e.g., by placing the substrate in a commercially available gravity or vacuum autoclave device, and the like. See, e.g., Evelina Palibroda, A. Lupsan, Stela Pruneanu, M. Savos, Thin Solid Films , 256, 101 (1995), the entire contents of which are incorporated by reference herein.
- acidic solution e.g., phosphoric, oxalic, or sulfuric acid solutions
- autoclaving the film layer e.g., by placing the substrate in a commercially available gravity or vacuum autoclave device, and the like. See, e.g., Evelina Palibroda, A. Lupsan, Stela Pruneanu, M. Savos, Thin
- textured surfaces other than alumina or aluminum can also be used as long as such surfaces enhance desorption sensitivity, including, for example, zinc oxide (ZnO) nanostructured surfaces described above.
- ZnO zinc oxide
- Low-temperature solution-based approaches to forming ZnO nanotextured surfaces are described, for example, in "Low Temperature Wafer-Scale Production of ZnO Nanowire Arrays," Lori E. Greene et al., Angew. Chem. Int. Ed. 2003, 42, 3031-3034, the entire contents of which are incorporated by reference herein.
- the supporting substrate 10 may be made from a non-silicon material such as aluminum from which a nanostructured surface can be formed (e.g., by heating the material with water or water vapor at a temperature greater than about 50 degrees Celsius, e.g., between about 50 to 150 degrees Celsius, e.g., at a temperature of from about 90 to 125 degrees Celsius, for a period of time sufficient to form the nanostructured surface, e.g., between about 3 and 60 minutes, e.g., preferably greater than about 5 minutes), and the active layer is deposited on the nanostructured surface of the substrate.
- a non-silicon material such as aluminum from which a nanostructured surface can be formed
- Figures 8A-D show mass spectrometry analysis of 25 picograms of four pharmaceutical compounds (e.g., clonidine, haloperidol, prazosin, and quinidine, respectively) deposited on a silicon coated nanostructured aluminum substrate surface.
- four pharmaceutical compounds e.g., clonidine, haloperidol, prazosin, and quinidine, respectively
- the active layer 24 on top of the nanostructured film layer may comprise a semiconductor which absorbs light energy such as IR, red, green, blue and/or UV laser light energy.
- the semiconductor may be selected from Group IV semiconductors including Si, Ge, diamonds and diamond-like carbon coatings, or compound semiconductors of Group H-V, II- VI and other semiconductors including sulfides, selenides, tellurides, nitrides, carbides, nitrides, antimonides, and phosphides.
- the active layer may also comprise a metal such as tungsten (W) or other metal which is catalytically active and that interferes with light absorption, and which is capable of promoting or enhancing light energy transfer to an analyte, e.g., to ionize and desorb it from the surface of the supporting substrate.
- the metal may be selected from the group comprising transition metals including be, Co, Cr, Ni, Mo, W, V, Cu, Zn and precise metals including Ag, Au, Pt, Pd, Ru, Rh, or metal alloys thereof.
- the active layer preferably has a thickness of between about 5 and 200 nm, for example, between about 10 and 50 nm.
- the silicon active layer is deposited on the thin film layer by a chemical vapor deposition process or by other thermal evaporation method.
- a chemical vapor deposition process or by other thermal evaporation method.
- depositing the silicon active layer in a CVD oven at a temperature of about 480 degrees Celsius for a time period of between about 10 and 80 minutes, for example, between about 30 and 80 minutes provides the optimum mass spectrometry results.
- Figures 9A-B illustrate the difference in peak signal for mass spectrometry analysis of 25 picograms of haloperidol spotted onto a silicon coated nanostructured alumina surface that had silicon deposition times in the CVD oven of thirty minutes ( Figure 9A) and ten minutes ( Figure 9B). It will be appreciated that the haloperidol peak only appears on the silicon coated surface that was CVD deposited for about 30 minutes.
- the CVD deposition time (and hence silicon active layer thickness) plays an important role in the sensitivity of the mass spectrometry signal.
- the active layer can also optionally be coated or functionalized, e.g., to enhance or add specific properties.
- polymers, ceramics, or small molecules can optionally be used as coating materials.
- the optional coatings can impart characteristics such as water resistance, improved mechanical, optical (e.g., enhancement of light coupling) or electrical properties or specificities for certain analytes.
- the active layer may also be derivatized with one or more functional moieties (e.g., a chemically reactive group) to enhance laser desorption such as one or more silane groups, e.g., one or more per-fluorinated silane groups, or other coatings such as diamond coatings, a hydrocarbon molecule, a fluorocarbon molecule, or a short chain polymer of both types of molecules which may be attached to the active layer via silane chemistry.
- functional moieties e.g., a chemically reactive group
- silane groups e.g., one or more per-fluorinated silane groups
- other coatings such as diamond coatings, a hydrocarbon molecule, a fluorocarbon molecule, or a short chain polymer of both types of molecules which may be attached to the active layer via silane chemistry.
- Those of skill in the art will be familiar with numerous functionalizations and functionalization techniques which are optionally used herein (e.g., similar to those used in construction of separation columns,
- the analytes may be directly linked to the active layer surface, e.g., through silane groups, or may be coupled via linker binding groups or other appropriate chemical reactive groups to participate in linkage chemistries (derivitization) with linking agents such as, e.g., substituted silanes, diacetylenes, acrylates, acrylamides, vinyl, styryls, silicon oxide, boron oxide, phosphorus oxide, N-(3-aminopropyl)3-mercapto-benzamide, 3-aminopropyl- trimethoxysilane, 3-mercaptopropyl-trimethoxysilane, 3-maleimidopropyl-trimethoxysilane, 3- hydrazidopropyl-trimethoxysilane, trichloro-perfluoro octyl silane, hydroxysuccinimides, maleimides, haloacetyls, pyridyl disulfides, hydr
- the supporting substrate 10 may be made from any one of a number of conventional materials including stainless steel, glass, quartz, semiconductor materials such as a semiconductor wafer, silicon, polymers, ceramics etc.
- Samples of the substances (e.g., small molecules, proteins, peptides etc.) to be analyzed are optionally placed in contact with the active layer (e.g., directly or via the use or one or more functional moieties such as one or more silane groups described above) by conventional dispensing means such as pipetting, dot- printing etc.
- the active layer e.g., directly or via the use or one or more functional moieties such as one or more silane groups described above
- conventional dispensing means such as pipetting, dot- printing etc.
- Laser energy levels and pulse durations are also optionally optimized for analysis of the samples arrayed upon the nanostructured substrate surface.
- the different parameters are optionally modified depending upon, e.g., the specific molecules being detected.
- the laser energy used can optionally be adjusted (e.g., higher laser energy levels for peptides as opposed to small molecules).
- An interesting feature of the nanostructured surface of the present invention is that it requires relatively low laser energy (e.g., on the order of about 60 Hz) to desorb small molecules therefore reducing background ion interference.
- a mass spectrometry device e.g., for matrix-free analysis of small molecules such as proteins, peptides, or small molecule drugs
- a mass spectrometry device which comprises a supporting substrate having a first surface and a plurality of nanocrystals, e.g., semiconductor nanocrystals such as quantum dots, deposited on the surface of the substrate.
- the nanocrystals can be a gold nanoparticle, a cobalt nanoparticle, an iron oxide nanoparticle, or a semiconductor nanocrystal including a semiconductor material, such as a Group IV compound (e.g., Si, Ge), a Group II- VI compound, a Group H-V compound, a Group III- VI compound, a Group III-V compound, a Group IV-VI compound, a Group I-III-VI compound, a Group II-IV-VI compound, or a Group II- IV- V compound.
- a Group IV compound e.g., Si, Ge
- a Group II- VI compound e.g., Si, Ge
- a Group II- VI compound e.g., a Group H-V compound
- a Group III- VI compound e.g., a Group III-V compound
- a Group IV-VI compound e.g., a Group I-III-VI compound, a Group II-IV-VI compound, or a Group II- IV-
- the semiconductor material can be, for example, ZnS, ZnSe, ZnTe, CdS, CdSe, CdTe, HgS, HgSe, HgTe, AlN, AIP, AlAs, AlSb, GaN, GaP, GaAs, GaSb, GaSe, InN, InP, InAs, InSb, TIN, TIP, TIAs, TlSb, PbS, PbSe, PbTe, or mixtures thereof.
- the nanoparticle can be coated with one or more second materials such as a second metal, semimetal, polymer, or semiconductor material such as ZnO, ZnS, ZnSe, ZnTe, CdO, CdS, CdSe, CdTe, MgO, MgS, MgSe, MgTe, HgO, HgS, HgSe, HgTe, AIN, AIP, AlAs, AlSb, GaN, GaP, GaAs, GaSb, InN, InP, InAs, InSb, TIN, TIP, TIAs, TlSb, TlSb, PbS, PbSe, PbTe, or mixtures thereof.
- a second metal, semimetal, polymer, or semiconductor material such as ZnO, ZnS, ZnSe, ZnTe, CdO, CdS, CdSe, CdTe, MgO, MgS, MgSe, M
- the nanoparticles are spin-coated onto the substrate surface, and then plasma treated to generate a water adsorbable oxide surface.
- the nanoparticles can also be spotted from solution onto the substrate surface (e.g., alone or in combination with the molecules to be sampled such as low molecular weight proteins, peptides or small molecule drugs), or can be applied to the surface by any other suitable method known to those of ordinary skill in the art.
- the nanocrystals can be applied directly to a tissue section whereupon energy transfer occurs from the nanocrystals directly to molecules in the tissue sample (such as proteins or small molecule drugs) which would be desorbed and ionized for mass spectrometry analysis.
- the surface of the nanoparticles can be functionalized as necessary.
- the collection or population of nanocrystals is substantially monodisperse in size and/or shape. See, e.g., USPN 6,207,229 (March 27, 2001) and USPN 6,322,901 (November 27, 2001) to Bawendi et al. entitled "Highly luminescent color-selective materials," the entire contents of each of which are incorporated by reference herein.
- synthesis occurs as an initial nucleation event that takes place over seconds, followed by crystal growth at elevated temperature for several minutes.
- Parameters such as the temperature, types of surfactants present, precursor materials, and ratios of surfactants to monomers can be modified so as to change the nature and progress of the reaction.
- the temperature controls the structural phase of the nucleation event, rate of decomposition of precursors, and rate of growth.
- the organic surfactant molecules mediate both solubility and control of the nanocrystal shape.
- the ratio of surfactants to monomer, surfactants to each other, monomers to each other, and the individual concentrations of monomers strongly influence the kinetics of growth.
- Si or CdSe is used as the nanocrystal material, although other suitable semiconductor materials can be used as described above (e.g., InP, ZnS, and the like).
- the nanocrystals will absorb laser light of a particular wavelength, or a particular range of wavelengths, whereupon efficient energy transfer from the nanocrystal to the molecules deposited thereon or therewith can occur to desorb and ionize the molecules for mass spectrometry analysis.
- the ability to make nanocrystals out of different semiconductors, and to control their size and shape, allows tor the fabrication of nanocrystals that will absorb light from the UV, to visible, to near infrared (NIR), to infrared (IR) wavelengths.
- Nanocrystals for use in the present invention will suitably be less than about 100 nm in size, and down to less than about 2 nm in size.
- the nanocrystals of the present invention absorb ultraviolet light, e.g., at a wavelength of between about 300 and 400 nm.
- a device for enhanced surface area applications and/or hydrophobic surface applications which generally comprises a supporting substrate, a non-silicon film layer on at least a region of the surface, the film layer having a nanostructured surface, and a hydrophobic (or hydrophilic) coating deposited on the film layer.
- the hydrophobic coating may comprise, for example, a diamond-like carbon coating (e.g., an amorphous diamond film) or other coating which renders the surface of the device hydrophobic (or hydrophilic).
- the diamond-like carbon film can be applied to the nanostructured surface by, e.g., using pulsed laser ablation on a graphite target or by a plasma assisted CVD method at low temperature. When the deposited material is heated, such films lose their stress, yet retain their diamond-like properties.
- Such devices can be used in a variety of high surface area and/or hydrophobic or hydrophilic surface applications including those which are disclosed in greater detail in co- pending and related cases
- U.S. Patent Application Serial No. 10/828,100, filed April 19, 2004, which is a continuation-in-part of U.S. Patent Application Serial No. 10/661,381, filed September 12, 2003, which claims priority to U.S. Provisional Patent Application No. 60/463,766, filed April 17, 2003; and U.S. Patent Application Serial No. 10/833,944, filed April 27, 2004, which claims priority to U.S. Provisional Application Serial No. 60/466,229, filed April 28, 2003; and to U.S. Patent Application Serial No.
- the unique nanostructured surfaces disclosed herein can be used in, on or within various medical devices, such as clamps, valves, intracorporeal or extracorporeal devices (e.g., catheters), temporary or permanent implants, stents, vascular grafts, anastomotic devices, aneurysm repair devices, embolic devices, implantable devices (e.g., orthopedic implants) and the like.
- Such enhanced surfaces provide many enhanced attributes to the medical devices in, on, or within which they are used including, e.g., to prevent/reduce bio-fouling, increase fluid flow due to hydrophobicity, increase adhesion, biointegration, etc.
- nanostructured coatings can be used as surface coatings for touch screens such as for information kiosks, gaming/entertainment/media consoles, point-of-sale terminals, industrial and medical instrumentation, ATM machines, kiosks in retailing, personal computer monitor screens, automobile displays, and the like.
- the nanostructured films disclosed herein can be used to provide a surface for cell attachment, differentiation, and proliferation, as a substrate to promote cell growth, or as a substrate for DNA or protein microarrays, e.g., to hybridize nucleic acids, proteins and the like.
- the nanostructured films disclosed herein have applications in vivo for tissue grafting including osteoblasts, neuronal, glia, epidermal, fibroblast cells and the like.
- nanostructured coatings can also be combined in particular applications with other nanostructured components such as nanofibers or nanowires which can be grown on the surfaces and/or deposited thereon, to provide further attributes of increased adhesion, hydrophobicity, hydropholicity, conductivity (e.g., for electrical contact applications) and the like to the devices with which they are used.
- the following non-limiting example demonstrates the feasibility of using an alumina nanostructured underlying thin film coated with an active silicon layer to perform mass spectrometry analysis.
- the nanostructured surface used in these experiments is based on alumina. 50nm of alumina was sputtered onto a 40mm square polished stainless steel plate. The plate was exposed to boiling water for three minutes which converts the alumina into a highly structured surface.
- the plate was then oxygen plasma cleaned for 10 minutes before placing it into a CVD furnace where silicon was deposited on it for 70 minutes.
- Two steel plates without the nanostructured alumina were also placed into the furnace as controls, one with no coating (Fig. 5A) and the other with a silicon film layer deposited thereon but no underlying alumina nanostructured surface (Fig. 5B).
- Fig. 5A Two steel plates without the nanostructured alumina were also placed into the furnace as controls, one with no coating
- Fig. 5B Two steel plates without the nanostructured alumina were also placed into the furnace as controls, one with no coating (Fig. 5A) and the other with a silicon film layer deposited thereon but no underlying alumina nanostructured surface (Fig. 5B).
- trichloro-perfluoro octyl silane Gelst, Morrissville, PA
- FIG. 5A shows results of mass spectrometry analysis of samples on the stainless steel substrate without any nanostructured thin film layer or silicon film layer.
- Figure 5B shows results of mass spectrometry analysis of samples on a stainless steel substrate with a silicon layer and without any underlying nanostructured thin film layer.
- Figure 5C shows results of mass spectrometry analysis of samples on a stainless steel substrate with a thin nanostructured film layer and a silicon active layer according to the teachings of the present invention. As can be seen almost no signal can be detected on the steel or silicon treated surfaces, but with the underlying alumina nanostructured surface, the Prazosin peak is off scale representing more then a 10Ox improvement.
- Figures 6A-D show further examples of mass spectrometry analysis of 25 picograms of four pharmaceutical compounds (e.g., clonidine, haloperidol, prazosin, and quinidine, respectively) deposited on a silicon coated nanostructured alumina surface.
- Figures 7A-D show examples of mass spectrometry analysis of 2.5 picograms of those same four compounds deposited on a silicon coated nanostuctured alumina surface.
- the mass spectra demonstrate signal detection of all four molecules spotted at 25 pg and 2.5 pg, respectively.
- the following non-limiting example demonstrates the feasibility of using an aluminum nanostructured underlying thin film coated with an active tungsten layer to perform mass spectrometry analysis.
- the nanostructured surface used in these experiments is based on aluminum. 100 nm of aluminum was sputtered onto a 40mm square polished stainless steel plate. The plate was exposed to boiling water for five minutes which converts the aluminum into a highly structured surface.
- the plate was then oxygen plasma cleaned for 10 minutes before placing it into a high vacuum sputtering system (UHV Sputtering Inc., San Jose, CA) where tungsten was deposited on it to a depth of 50 nm.
- the plate was then plasma cleaned again and was exposed to trichloro-perfluoro octyl silane (Gelest, Morrisville, PA) vapor for three hours to render it hydrophobic.
- the plate was then washed thoroughly in methanol and baked for 20 minutes at 100 degrees Celsius.
- FIG. 10A-D show results of mass spectrometry analysis of 25 picograms of the four pharmaceutical compounds (e.g., prazosin, haloperidol, quinidine and clonidine, respectively) deposited on the tungsten coated nanostructured aluminum surface.
- the mass spectra demonstrate signal detection of all four molecules spotted at 25
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Cited By (9)
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WO2007117838A2 (en) * | 2006-03-30 | 2007-10-18 | Medtronic, Inc | Atomic layer deposition coatings for implantable medical devices |
WO2007117838A3 (en) * | 2006-03-30 | 2007-12-13 | Medtronic Inc | Atomic layer deposition coatings for implantable medical devices |
US8911834B2 (en) | 2007-11-05 | 2014-12-16 | Leibniz-Institut Fuer Neue Materialien Gemeinnuetzige Gmbh | Oxide compounds as a coating composition |
WO2009071035A2 (en) * | 2007-12-03 | 2009-06-11 | Masarykova Univerzita | Method of sample preparation for atomic spectrometric techniques |
WO2009071035A3 (en) * | 2007-12-03 | 2009-07-23 | Univ Masarykova | Method of sample preparation for atomic spectrometric techniques |
DE102009035795A1 (en) * | 2009-07-31 | 2011-02-03 | Leibniz-Institut Für Neue Materialien Gemeinnützige Gmbh | Structured surfaces for implants |
WO2011012213A3 (en) * | 2009-07-31 | 2011-06-03 | Leibniz-Institut Für Neue Materialien Gemeinnützige Gmbh | Structured surfaces for implants |
WO2018134232A1 (en) * | 2017-01-19 | 2018-07-26 | École Polytechnique Fédérale de Lausanne | Maldi device and methods of preparation and use thereof |
EP4165679A4 (en) * | 2020-06-15 | 2024-07-17 | Univ Monash | Method for detecting analytes |
Also Published As
Publication number | Publication date |
---|---|
WO2006118595A3 (en) | 2007-11-29 |
US7755038B2 (en) | 2010-07-13 |
AU2005331265B2 (en) | 2010-03-04 |
CA2578359A1 (en) | 2006-11-09 |
US7391018B2 (en) | 2008-06-24 |
US20090124034A1 (en) | 2009-05-14 |
AU2005331265A1 (en) | 2006-11-09 |
EP1804958A2 (en) | 2007-07-11 |
JP2008513781A (en) | 2008-05-01 |
US20080073505A1 (en) | 2008-03-27 |
EP1804958A4 (en) | 2010-11-17 |
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