WO2006111757A3 - Animal model of vascular smooth muscle cell apoptosis - Google Patents

Animal model of vascular smooth muscle cell apoptosis Download PDF

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Publication number
WO2006111757A3
WO2006111757A3 PCT/GB2006/001450 GB2006001450W WO2006111757A3 WO 2006111757 A3 WO2006111757 A3 WO 2006111757A3 GB 2006001450 W GB2006001450 W GB 2006001450W WO 2006111757 A3 WO2006111757 A3 WO 2006111757A3
Authority
WO
WIPO (PCT)
Prior art keywords
smooth muscle
animal model
vascular smooth
muscle cell
cell apoptosis
Prior art date
Application number
PCT/GB2006/001450
Other languages
French (fr)
Other versions
WO2006111757A2 (en
Inventor
Martin Bennett
Murray Clarke
Trevor Littlewood
Original Assignee
Univ Cambridge Tech
Martin Bennett
Murray Clarke
Trevor Littlewood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cambridge Tech, Martin Bennett, Murray Clarke, Trevor Littlewood filed Critical Univ Cambridge Tech
Publication of WO2006111757A2 publication Critical patent/WO2006111757A2/en
Publication of WO2006111757A3 publication Critical patent/WO2006111757A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases

Abstract

This invention relates to non-human animal model which expresses a heterologous nucleic acid encoding a receptor for an apoptotic agent, such as diphtheria toxin (DT), specifically in Vascular Smooth Muscle Cells (VSMCs) of blood vessel walls. This allows apoptosis to be induced with very high specificity in these VSMCs and provides a useful model system for a range of vascular conditions, including angiogenesis, calcification and atherosclerosis.
PCT/GB2006/001450 2005-04-20 2006-04-20 Animal model of vascular smooth muscle cell apoptosis WO2006111757A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0507983.5 2005-04-20
GBGB0507983.5A GB0507983D0 (en) 2005-04-20 2005-04-20 Apoptosis model

Publications (2)

Publication Number Publication Date
WO2006111757A2 WO2006111757A2 (en) 2006-10-26
WO2006111757A3 true WO2006111757A3 (en) 2006-12-14

Family

ID=34630990

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/001450 WO2006111757A2 (en) 2005-04-20 2006-04-20 Animal model of vascular smooth muscle cell apoptosis

Country Status (2)

Country Link
GB (1) GB0507983D0 (en)
WO (1) WO2006111757A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008106982A2 (en) * 2007-03-07 2008-09-12 Aarhus Universitet Pig model for atherosclerosis
US10568974B2 (en) * 2012-02-07 2020-02-25 Exemplar Genetics Llc Animal models of atherosclerosis
CN115433776B (en) * 2022-09-30 2023-12-22 中国医学科学院阜外医院 Application of CCN3 in regulating vascular smooth muscle cell calcification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197756B1 (en) * 1998-10-19 2001-03-06 Texas Heart Institute Gene transfer of E2F-1 to inhibit vascular smooth muscle cell accumulation in vascular tissue
US6331527B1 (en) * 1995-10-05 2001-12-18 Arch Development Corporation Promoter smooth muscle cell expression
US20020194626A1 (en) * 1997-01-31 2002-12-19 Kenji Kohno Knockout animals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331527B1 (en) * 1995-10-05 2001-12-18 Arch Development Corporation Promoter smooth muscle cell expression
US20020194626A1 (en) * 1997-01-31 2002-12-19 Kenji Kohno Knockout animals
US6197756B1 (en) * 1998-10-19 2001-03-06 Texas Heart Institute Gene transfer of E2F-1 to inhibit vascular smooth muscle cell accumulation in vascular tissue

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BENNETT M R: "Role of macrophages in atherogenesis and established atherosclerosis", IMMUNOLOGY, vol. 116, no. Suppl. 1, December 2005 (2005-12-01), & ANNUAL CONGRESS OF THE BRITISH-SOCIETY-FOR-IMMUNOLOGY; HARROGATE, ENGLAND; DECEMBER 06 -09, 2005, pages 59, XP002397238, ISSN: 0019-2805 *
JUNG STEFFEN ET AL: "In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens.", IMMUNITY. AUG 2002, vol. 17, no. 2, August 2002 (2002-08-01), pages 211 - 220, XP002397258, ISSN: 1074-7613 *
LITTLEWOOD TREVOR D ET AL: "Apoptotic cell death in atherosclerosis.", CURRENT OPINION IN LIPIDOLOGY. OCT 2003, vol. 14, no. 5, October 2003 (2003-10-01), pages 469 - 475, XP009071652, ISSN: 0957-9672 *
M. CLARKE ET AL: "Conditional ablation of vascular smooth muscle cells: direct effects of cell death on normal arteries and in atherosclerosis", JOURNAL OF VASCULAR RESEARCH, SUPPLEMENT 2, vol. 42, September 2005 (2005-09-01), The 3rd European Meeting on Vascular Medicine and Biology, pages II/117 - II/118, XP002397259, ISBN: 3-8055-8035-5, Retrieved from the Internet <URL:http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowPDF&ProduktNr=224160&Ausgabe=231361&ArtikelNr=89079&filename=89079.pdf> [retrieved on 20060831] *
MAJESKY MARK W: "Mouse model for atherosclerotic plaque rupture.", CIRCULATION. 30 APR 2002, vol. 105, no. 17, 30 April 2002 (2002-04-30), pages 2010 - 2011, XP002397236, ISSN: 1524-4539 *
MURRAY C H CLARKE ET AL: "Apoptosis of vascular smooth muscle cells induces features of plaque vulnerability in atherosclerosis.", NATURE MEDICINE, 6 August 2006 (2006-08-06), XP002396893, ISSN: 1078-8956 *
SAITO M ET AL: "Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 19, no. 8, August 2001 (2001-08-01), pages 746 - 750, XP002994353, ISSN: 1087-0156 *
VON DER THÜSEN JAN H ET AL: "Induction of atherosclerotic plaque rupture in apolipoprotein E-/- mice after adenovirus-mediated transfer of p53.", CIRCULATION. 30 APR 2002, vol. 105, no. 17, 30 April 2002 (2002-04-30), pages 2064 - 2070, XP002397237, ISSN: 1524-4539 *
ZHAO ZHIHUI ET AL: "Overexpression of A3 adenosine receptors in smooth, cardiac, and skeletal muscle is lethal to embryos.", MICROVASCULAR RESEARCH. JAN 2002, vol. 63, no. 1, January 2002 (2002-01-01), pages 61 - 69, XP002396881, ISSN: 0026-2862 *

Also Published As

Publication number Publication date
WO2006111757A2 (en) 2006-10-26
GB0507983D0 (en) 2005-05-25

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