WO2006096063B1 - Construction et methode pour cribler, selectionner et identifier des facteurs de stress - Google Patents

Construction et methode pour cribler, selectionner et identifier des facteurs de stress

Info

Publication number
WO2006096063B1
WO2006096063B1 PCT/NL2006/050051 NL2006050051W WO2006096063B1 WO 2006096063 B1 WO2006096063 B1 WO 2006096063B1 NL 2006050051 W NL2006050051 W NL 2006050051W WO 2006096063 B1 WO2006096063 B1 WO 2006096063B1
Authority
WO
WIPO (PCT)
Prior art keywords
compound
promoter
gram negative
concentration
family
Prior art date
Application number
PCT/NL2006/050051
Other languages
English (en)
Other versions
WO2006096063A1 (fr
Inventor
Janelle L Brown
Klaas J Hellingwerf
Wim Crielaard
Original Assignee
Stichting Tech Wetenschapp
Janelle L Brown
Klaas J Hellingwerf
Wim Crielaard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stichting Tech Wetenschapp, Janelle L Brown, Klaas J Hellingwerf, Wim Crielaard filed Critical Stichting Tech Wetenschapp
Publication of WO2006096063A1 publication Critical patent/WO2006096063A1/fr
Publication of WO2006096063B1 publication Critical patent/WO2006096063B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des constructions et des méthodes pour cribler, pour sélectionner et pour identifier des facteurs de stress perturbant la bioénergie d'une cellule vivante, en particulier la bioénergie de la cellule microbienne. Dans un aspect de l'invention, l'invention concerne un promoteur présentant une activité corrélée, pouvant servir d'indicateur de changement du niveau bioénergétique de la cellule. L'invention concerne des constructions de fusion et des hôtes comprenant ces constructions pouvant être utilisés dans la méthode de l'invention. Ainsi, l'invention permet un criblage, une sélection et une identification rapides de facteurs de stress de la cellule, notamment des composés antimicrobiens et des traitements antimicrobiens. Ces facteurs de stress peuvent être criblés séparément ou combinés pour obtenir une concentration optimalement efficace.
PCT/NL2006/050051 2005-03-11 2006-03-10 Construction et methode pour cribler, selectionner et identifier des facteurs de stress WO2006096063A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05075601.4 2005-03-11
EP05075601 2005-03-11

Publications (2)

Publication Number Publication Date
WO2006096063A1 WO2006096063A1 (fr) 2006-09-14
WO2006096063B1 true WO2006096063B1 (fr) 2006-11-23

Family

ID=34938096

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2006/050051 WO2006096063A1 (fr) 2005-03-11 2006-03-10 Construction et methode pour cribler, selectionner et identifier des facteurs de stress

Country Status (1)

Country Link
WO (1) WO2006096063A1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69316368T2 (de) * 1992-07-06 1998-06-25 Harvard College Verfahren und diagnostische kits zur bestimmung der toxizität einer verbindung unter verwendung von an bakterielle stresspromotoren fusionierten reportergenen
IL107815A (en) * 1992-12-04 2003-12-10 Du Pont Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing
CA2200702C (fr) * 1994-11-23 2000-12-05 E.I. Du Pont De Nemours And Company Reactif bacterien bioluminescent lyophilise servant a la detection d'agents toxiques
JP2002505120A (ja) * 1998-03-06 2002-02-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 抗菌活性を有するポリカチオン性ペプチド様化合物を同定する方法
US6333187B1 (en) * 1998-09-30 2001-12-25 Centrum Voor Plantenveredelings-En Collection of phagemids, and a collection of E. coli cells carrying the phagemids

Also Published As

Publication number Publication date
WO2006096063A1 (fr) 2006-09-14

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