WO2006096054A1

WO2006096054A1 - Multifold oxygen measurement device

Info

Publication number: WO2006096054A1
Application number: PCT/NL2006/000120
Authority: WO
Inventors: Albert Van Duijn; Wessel Luitje Holtman; Arie Draaijer
Original assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
Priority date: 2005-03-09
Filing date: 2006-03-08
Publication date: 2006-09-14
Also published as: CN101160042A; CA2600457A1; AU2006221188A1; ATE540573T1; US20090126268A1; ZA200708255B; AU2006221188B2; EP1860931B1; CA2600457C; WO2006096054A8; BRPI0608697A2; EP1860931A1; EP1700520A1; PL1860931T3

Abstract

The invention relates to a plant cultivating environment comprising: an oxygen sensing arrangement for sensing an oxygen level in said environment. According to the invention, said oxygen sensing arrangement comprises a base plate for underlying a substrate; and a plurality of oxygen sensors each positioned on said base plate at a predetermined position for sensing a local oxygen level in said substrate. In this way, a water irrigation strategy can be based on sensed levels at a plurality of positions.

Description

Multifold oxygen measurement device.

The invention relates to a plant cultivating environment comprising: an oxygen sensing arrangement for sensing an oxygen level in said environment.

For plant cultivating environments, WO03005807 discusses various aspects related to monitoring and improving growth conditions. One of these aspects is measuring an oxygen level in a plant substrate in order to adequately adjust the oxygen level to improve the growth conditions in the substrate. A sensor that is proposed to implement such oxygen monitoring is the sensor described in WO01/63264, utilizing a fluorescence quenching effect by oxygen. This sensor can be used as a fluorescent coating to be brought in contact with the oxygen environment and by a photoelectric readout of the fluorescence an oxygen level can be inferred.

One of the aspects that is discussed is the fact that water level is an important factor for further assessing the oxygenation of the substrate. Another aspect that is touched upon is the aspect that oxygen levels may vary from place to place. It has been found that the existing oxygen sensor is only capable of measuring a single spot oxygen level, so that adequate measuring of oxygen levels is cumbersome due to the need of repeated measurements or unreliable, due to the limited number of spots that are selectable and expensive due to the equipment needed to provide a plurality of simultaneous measurements.

To overcome the above drawbacks, the invention provides a plant cultivating environment according to the features of claim 1. In particular, a plant cultivating environment is provided comprising: an oxygen sensing arrangement for sensing an oxygen level in said environment; said oxygen sensing arrangement comprising a base plate for underlying a substrate; and a plurality of oxygen sensors each positioned on said base plate at a predetermined position for sensing a local oxygen level in said substrate. In a preferred embodiment the oxygen sensors are dimensioned differing in height relative to the base plate. It has been found that the oxygen levels may vary dramatically relative to the height of the base plate and that an irrigation strategy may be heavily dependent on the particular oxygen levels sensed at differing heights.

In another aspect, the invention provides a method of cultivating plants in a plant cultivating environment comprising: providing an oxygen sensing arrangement for sensing an oxygen level in a substrate for cultivating plants; measuring an oxygen level at plurality of predetermined positions in said substrate for sensing a local oxygen level in said substrate; and adjusting a water irrigation in said substrate in response to a measured oxygen level at said plurality of predetermined positions. Here preferably, depending on a sensed oxygen level at a predetermined height in the substrate the method may comprise draining water from the substrate and/or supplying water to the substrate to regulate oxygen levels in the substrate.

Specifically the method may comprise draining water from the substrate when an oxygen level is low in a higher part of the substrate. Alternatively, or in addition the method comprises supplying water from the substrate when an oxygen level is low in a lower part of the substrate.

Further aspects and benefits of the invention will be described with reference to the drawings. Here:

Figure 1 shows a schematic drawing of a plant cultivating environment according to the invention; Figure 2 shows a graph illustrating a difference in draining (A) and supplying of water (B); and

Figure 3 shows a graph illustrating an effect of locally supplying water in two (A) or three (B) positions; and

Figure 4 shows a correlation diagram of a measured averaged Oxygen content with various other growth factors relevant for plant cultivation. In Figure 1 a container 1, for use in a greenhouse or other plant growing environment is shown that is adapted according to the invention to provide a simultaneous or almost simultaneous (for instance: sequential) measurement of oxygen levels in a substrate 2. The substrate 2 is typically a conventional substrate such as mineral growth substrate: glass wool or rock wool. Plants 3 are cultivated on the substrate 2 by supplying water and other growth factors. Water is typically provided via water supplies 4. To adequately monitor growth conditions, an oxygen measurement device 5 is present which is coupled to oxygen sensors 6. The oxygen measurement device 5 is arranged as a processor processing multiple input signals, which may be of an electrical or optical nature. In the preferred embodiment, the sensors 6 are arranged, as schematically depicted, as optical fibres 7 (glass fibres), which are at a distal end coated with an oxygen sensitive coating 8, in particular, a fluorescent dye that is sensitive to oxygen levels. Typically, such a dye can be a matrix material in which an organometallic complex is embedded.

The organometallic complex is an oxygen-sensitive fluorescent dye, with the amount of fluorescence and the fluorescence life time being dependent on the oxygen content in the medium. Such an organometal typically consists of Tris Ru2+-4,7-biphenyl-l,10-phenanthroline; this Ru(ruthenium) complex is particularly oxygen-sensitive, but other organometals can also be used, such as an Os complex or a Pt complex. Because of the gas permeability of the substrate 2, oxygen can interact with the organometallic complex. As a result, the amount of fluorescence is influenced by the amount of oxygen in the medium. By measuring the emitted intensity or life time of the fluorescence, the extent of the influence, and hence the oxygen content, can be established.

A typical way to measure an oxygen level is to generate light, preferably by a lamp (not depicted) in the oxygen measurement device 5, of a specific wavelength to which the fluorescent dye is sensitive. Through optic fibre 7 this light propagates to the fluorescent coating 8, which, under the influence of oxygen, shows a specific fluorescent behaviour as explained above. This fluorescence can be conveniently measured by guiding the fluorescent light via the same fibre 7 back to the oxygen measurement device 5 where it can be photo electrically converted to provide an electric sensing signal. This signal can be fed into an AD converter and further processed by processor to calculate an oxygen level. Further, this information may be combined by the processor with position information which may be inherently available in a memory element. To this end, the fibre may be identified based on a predetermined position order or other identification characteristic and this identification can be coupled to available position information. Alternatively, the sensor 6 may be provided with positioning means and a signal circuit which provides positioning information to the oxygen measurement device 5.

To secure the sensors in the substrate 2, according to the invention, the sensors are positioned on a base plate 9 at a predetermined position for sensing a local oxygen level in the substrate 2. This can be done, preferably, by mounting guide elements 10, such metal pipes or the like, on a base plate 9 and feeding the optical fibre therethrough until a predetermined height. Alternatively, the base plate 9 may be produced in plastic, for instance, by plastic moulding, and plate 9 and guides 10 may be formed monolithically. Furthermore, to provide a conveniently manageable embodiment, the fibres may be provided with a connector element 11 which couples the fibres individually or in common to an optical input/output 12 of the oxygen measurement device 5. In the device 5, the fibres may be coupled to an photoelectric array element such as a linear or matrix element. The output thereof can be spatially separated to belong to different fibres, each identifiable to a predetermined spatial position in the substrate 2. Alternative mechanical or optical switching may be provided to select one or more fibres for processing. Further, alternatively, a simultaneous or sequential processing may be provided by multiplexing or parallel processing of optical signals derived from a plurality of fibres. As depicted in a preferred embodiment in Figure 1, apart from monitoring, direct control can be provided, as shown by way of example in the embodiment depicted in Figure 1, to drains 13 and/or supplies 4 in order to adjust a water irrigation regime to the substrate. In Figure 2 and Figure 3 can be shown how measurement of local oxygen levels may be used to adjust the irrigation regime.

In particular, Figure 2 illustrates a measurement that was performed in a conventional rock wool substrate of about 1x2 m. In the substrate, on forty spatially differing positions an oxygen level was measured, on 15 linear positions along the length of the substrate. In the graph height positions are indicated as a, b, c, or d positions, wherein a is a lower position and d is a higher position just below the surface of the substrate. In the measurement arrangement, the sensors were formed by metal pins of differing heights, that were coated with a fluorescent coating as discussed above sensitive to an oxygen level. The fluorescence of the coating was measured by locally irradiating the coating and measuring the fluorescence decay of the coating. In the Figure 2A situation, the substrate was immersed for 95% with oxygen low (oxygen depleted) water as an initial starting situation. Then, in the Figure 2A situation, the substrate was drained, from 95% to 80 immersion. Figure 2A shows on the X-axis the position sensors sorted to height

(lowest a- and b-sensors on the left, higher c- and d-sensors on the right). On the Y-axis an increase of oxygen level is illustrated in percentages. As is clearly shown, in the higher regions (bed positions, in the graph sorted to the right) a considerable (around 4%) positive increase of oxygen content is measured. The lower regions marginally profit from draining the substrate.

Next, oxygen rich water was supplied to the substrate. Figure 2B clearly shows that this uniformly affects the oxygen level distribution, especially, also on the lower heights (a-, b-sensors) oxygen level increases. It can be shown, that , depending on oxygen distribution, supplying or draining the substrate may affect the oxygen levels in the substrate and the oxygenation of higher or lower parts of the substrate in a vertical direction can be controlled.

Figure 3 shows the locality of water supply and how this can influence the oxygen distribution in the horizontal plane. In Figure 3A water is supplied on two positions (the 5 and 10 positions, located at about 1/3 and 2/3 of the substrate length). An oxygen increase is clearly shown in the vertical direction around the 5 position (positions 4d, 5a-c and 6a-c show a significant increase in oxygenation) and the 10 position.

In Figure 3 B the same routine was exercised for three different supply positions, in particular, the 2, 8 and 14 position. Again, clearly, the 2-4, the 7-9 and the 13-14 positions show a positive increase in oxygen level.

In Figure 4 a correlation diagram is shown indicating a strong correlation between an averaged oxygen (02) content at a predetermined height in a cultivation substrate (measured at a plurality of positions at 1 cm from the bottom of the substrate). It clearly shows a strong correlation with among others light (LI, strong negative correlation), temperature of the substrate (MatTemp), time of the day (Time), and water content (WG). The striped areas indicate a negative correlation. The temperature of the substrate and the water content of the substrate, as well as the light intensity in the green house, appears to be negatively correlated to the oxygen content: the less light and the colder and less water in the substrate, the higher is the oxygen content.

Although the invention has been illustrated with the specific embodiment of Figure 1, variations and modification to that embodiment fall well within the scope of the claims. Such variations may be tables, soil, or concrete floors. Furthermore, besides mineral growth substrate, also other substrates such as peat, coir etc fall within the scope of the claim. The substrates may be present as slabs, pots, or blocks. Variations may also comprise: electrical transmission of oxygen sensing signals; local readout of the sensors through a probe that is brought in contact with the sensors, for instance, a probe that contacts an oxygen sensitive coating applied on cylindrical pins erected on a base plate. Such variations or modification are deemed to be part of the invention as claimed in the annexed claims.

Claims

1. A plant cultivating environment comprising:

— an oxygen sensing arrangement for sensing an oxygen level in said environment; said oxygen sensing arrangement comprising:

— a base plate for underlying a substrate; and — a plurality of oxygen sensors each positioned on said base plate at a predetermined position for sensing a local oxygen level in said substrate.

2. A plant cultivating environment according to claim 1, wherein the oxygen sensors are dimensioned differing in height relative to the base plate.

3. A plant cultivating environment according to claim 1 or 2, wherein the sensors are provided at a distal end of optical fibres that are oriented perpendicularly at predetermined positions relative to the base plate, the fibres connectable to a read out device.

4. A plant cultivating environment according to claim 3, wherein the fibres are inserted in cylindrical guides that are fixed relative to the base plate.

5. A plant cultivating environment according to any of the preceding claims, further comprising a read out device connectable to the oxygen sensor for reading out an optical output of said oxygen sensor and a switching device for switching a predetermined one of said plurality of oxygen sensors to said read out device.

_ 6. A plant cultivating environment according to any of the preceding ' . claims, wherein the oxygen sensor comprises an oxygen sensitive fluorescent dye that under the influence of incident light, provides fluorescence, which can be read out in a read out device, wherein a fluorescence lifetime is associated with a sensed oxygen level.

7. A method of cultivating plants in a plant cultivating environment comprising: - providing an oxygen sensing arrangement for sensing an oxygen level in a substrate for cultivating plants;

— measuring an oxygen level at plurality of predetermined positions in said substrate for sensing a local oxygen level in said substrate; and

- adjusting a water irrigation in said substrate in response to a measured oxygen level at said plurality of predetermined positions.

8. A plant cultivating method according to claim 7, wherein the oxygen sensors are dimensioned differing in height relative to a base plate.

9. A method according to any of the claims 7 and 8, comprising draining water from the substrate and/or supplying water to the substrate to regulate oxygen levels in the substrate.