WO2006083514A3 - Transgenic production of spider silk - Google Patents

Transgenic production of spider silk Download PDF

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Publication number
WO2006083514A3
WO2006083514A3 PCT/US2006/001078 US2006001078W WO2006083514A3 WO 2006083514 A3 WO2006083514 A3 WO 2006083514A3 US 2006001078 W US2006001078 W US 2006001078W WO 2006083514 A3 WO2006083514 A3 WO 2006083514A3
Authority
WO
WIPO (PCT)
Prior art keywords
spider silk
transgenic production
production
transgenic
silk
Prior art date
Application number
PCT/US2006/001078
Other languages
French (fr)
Other versions
WO2006083514A2 (en
Inventor
Rene J Herrera
Jose L Coll
Juan C Almagro
Original Assignee
Florida Internat University Bo
Rene J Herrera
Jose L Coll
Juan C Almagro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Florida Internat University Bo, Rene J Herrera, Jose L Coll, Juan C Almagro filed Critical Florida Internat University Bo
Publication of WO2006083514A2 publication Critical patent/WO2006083514A2/en
Publication of WO2006083514A3 publication Critical patent/WO2006083514A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Materials and methods are provided for the production of recombinant spider silk in a transgenic insect.
PCT/US2006/001078 2005-01-14 2006-01-13 Transgenic production of spider silk WO2006083514A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64408605P 2005-01-14 2005-01-14
US60/644,086 2005-01-14

Publications (2)

Publication Number Publication Date
WO2006083514A2 WO2006083514A2 (en) 2006-08-10
WO2006083514A3 true WO2006083514A3 (en) 2007-01-18

Family

ID=36777725

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/001078 WO2006083514A2 (en) 2005-01-14 2006-01-13 Transgenic production of spider silk

Country Status (1)

Country Link
WO (1) WO2006083514A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006333611B2 (en) 2005-12-30 2012-04-26 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
CN105400815B (en) * 2015-10-23 2019-01-25 杭州超丝生物科技有限公司 The method for synthesizing secretion latrodectus mactans traction silk-fibroin 1 using domestic natural silk gland bioreactor
CN105463022B (en) * 2015-10-23 2019-01-25 杭州超丝生物科技有限公司 The method for synthesizing secretion latrodectus mactans traction silk-fibroin 2 using domestic natural silk gland bioreactor
CN105400817B (en) * 2015-10-23 2019-01-18 杭州超丝生物科技有限公司 Utilize the method for the silkworm simultaneously synthesizing traction of secretion latrodectus mactans silk-fibroin 1 and albumen 2

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020137211A1 (en) * 2001-01-02 2002-09-26 Sichuan University Tianyou Biologic Engineering Co. Ltd. Method for establishing an expression system of spider dragline silk gene in bombyx mori

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020137211A1 (en) * 2001-01-02 2002-09-26 Sichuan University Tianyou Biologic Engineering Co. Ltd. Method for establishing an expression system of spider dragline silk gene in bombyx mori

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ASAKURA TETSUO ET AL: "Synthesis and characterization of chimeric silkworm silk.", BIOMACROMOLECULES, vol. 4, no. 3, 29 March 2003 (2003-03-29), pages 815 - 820, XP002397964, ISSN: 1525-7797 *
WANG ET AL: "In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 341, no. 4, 24 March 2006 (2006-03-24), pages 1203 - 1210, XP005283014, ISSN: 0006-291X *
WINKLER S ET AL: "Molecular biology of spider silk", REVIEWS IN MOLECULAR BIOTECHNOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 74, no. 2, August 2000 (2000-08-01), pages 85 - 93, XP002992190, ISSN: 1389-0352 *

Also Published As

Publication number Publication date
WO2006083514A2 (en) 2006-08-10

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