WO2006083292A3 - Microorganism detection using bacteriophage amplification - Google Patents

Microorganism detection using bacteriophage amplification Download PDF

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Publication number
WO2006083292A3
WO2006083292A3 PCT/US2005/020248 US2005020248W WO2006083292A3 WO 2006083292 A3 WO2006083292 A3 WO 2006083292A3 US 2005020248 W US2005020248 W US 2005020248W WO 2006083292 A3 WO2006083292 A3 WO 2006083292A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacteriophage
sample
nucleic acid
microorganism
protein
Prior art date
Application number
PCT/US2005/020248
Other languages
French (fr)
Other versions
WO2006083292A2 (en
Inventor
John H Wheeler
Jon Rees
G Scott Gaisford
Original Assignee
Microphage Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microphage Inc filed Critical Microphage Inc
Priority to EP05856796A priority Critical patent/EP1789572A2/en
Publication of WO2006083292A2 publication Critical patent/WO2006083292A2/en
Publication of WO2006083292A3 publication Critical patent/WO2006083292A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of detecting the presence or absence of a target microorganism in a sample to be tested comprising: combining with the sample, bacteriophage capable of infecting the target microorganism to create a bacteriophage exposed sample; providing conditions to the bacteriophage exposed sample sufficient to allow the bacteriophage to infect the target microorganism to create injected bacteriophage nucleic acid, additional bacteriophage nucleic acid, intermediate bacteriophage protein, or additional bacteriophage protein; and assaying the nucleic acid or protein to determine the presence or absence of the target microorganism. The microorganism is preferably dissociated or lysed prior to the completion of the bacteriophage replication process. The sample is an insitu sample and the microorganism is isolated from the insitu matrix. The assaying comprises comparing the test nucleic acid or protein level to a reference level.
PCT/US2005/020248 2004-06-07 2005-06-07 Microorganism detection using bacteriophage amplification WO2006083292A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05856796A EP1789572A2 (en) 2004-06-07 2005-06-07 Microorganism detection using bacteriophage amplification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57784504P 2004-06-07 2004-06-07
US60/577,845 2004-06-07
US60794104P 2004-09-07 2004-09-07
US60/607,941 2004-09-07

Publications (2)

Publication Number Publication Date
WO2006083292A2 WO2006083292A2 (en) 2006-08-10
WO2006083292A3 true WO2006083292A3 (en) 2007-04-26

Family

ID=36709988

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/020248 WO2006083292A2 (en) 2004-06-07 2005-06-07 Microorganism detection using bacteriophage amplification

Country Status (2)

Country Link
EP (1) EP1789572A2 (en)
WO (1) WO2006083292A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9441204B2 (en) 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
EP2087130A2 (en) * 2006-10-31 2009-08-12 Microphage, Incorporated Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms
JP5150856B2 (en) 2007-06-15 2013-02-27 マイクロファージ・インコーポレーテッド Microbial detection method with improved bacteriophage amplification
WO2012158041A1 (en) 2011-05-18 2012-11-22 Rna Holding B.V. Diagnostic methods and kits for determining the presence of a microorganism in a sample
WO2023122608A1 (en) * 2021-12-21 2023-06-29 Adaptive Phage Therapeutics, Inc. Assay for matching phage to bacteria

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888725A (en) * 1992-09-22 1999-03-30 The Secretary Of State For The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method for identifying target bacteria
WO2003087772A2 (en) * 2002-04-12 2003-10-23 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888725A (en) * 1992-09-22 1999-03-30 The Secretary Of State For The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method for identifying target bacteria
WO2003087772A2 (en) * 2002-04-12 2003-10-23 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FAVRIN S J ET AL: "Development and optimization of a novel immunomagnetic separation-bacteriophage assay for detection of Salmonella enterica serovar enteritidis in broth", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 67, no. 1, January 2001 (2001-01-01), pages 217 - 224, XP002996220, ISSN: 0099-2240 *
HIRSH D C ET AL: "RAPID DETECTION OF SALMONELLA SPP. BY USING FELIX-O1 BACTERIOPHAGE AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 45, no. 1, January 1983 (1983-01-01), pages 260 - 264, XP009033075, ISSN: 0099-2240 *
MADONNA A J ET AL: "DETECTION OF ESCHERICHIA COLI USING IMMUNOMAGNETIC SEPARATION AND BACTERIOPHAGE AMPLIFICATION COUPLED WITH MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, HEYDEN, LONDON, GB, vol. 17, no. 3, 2003, pages 257 - 263, XP009042142, ISSN: 0951-4198 *
STEWART G S A B ET AL: "THE SPECIFIC AND SENSITIVE DETECTION OF BACTERIAL PATHOGENS WITHIN 4 H USING BACTERIOPHAGE AMPLIFICATION", JOURNAL OF APPLIED MICROBIOLOGY, OXFORD, GB, vol. 84, May 1998 (1998-05-01), pages 777 - 783, XP000979801, ISSN: 1364-5072 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9441204B2 (en) 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria

Also Published As

Publication number Publication date
WO2006083292A2 (en) 2006-08-10
EP1789572A2 (en) 2007-05-30

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