WO2006081976A1 - Mutants de la proteine fluorescente cgfp et leur utilisation - Google Patents

Mutants de la proteine fluorescente cgfp et leur utilisation Download PDF

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Publication number
WO2006081976A1
WO2006081976A1 PCT/EP2006/000669 EP2006000669W WO2006081976A1 WO 2006081976 A1 WO2006081976 A1 WO 2006081976A1 EP 2006000669 W EP2006000669 W EP 2006000669W WO 2006081976 A1 WO2006081976 A1 WO 2006081976A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
cgfp
fluorescent
marker
nucleic acid
Prior art date
Application number
PCT/EP2006/000669
Other languages
German (de)
English (en)
Inventor
Stefan Golz
Eugene Vysotski
Svetlana Markova
Ludmilla Burakova
Ludmilla Frank
Original Assignee
Bayer Healthcare Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare Ag filed Critical Bayer Healthcare Ag
Publication of WO2006081976A1 publication Critical patent/WO2006081976A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne la séquence nucléotidique et la séquence d'acides aminés, ainsi que l'activité et l'utilisation de mutants de la protéine fluorescente CGFP.
PCT/EP2006/000669 2005-02-05 2006-01-26 Mutants de la proteine fluorescente cgfp et leur utilisation WO2006081976A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200510005438 DE102005005438A1 (de) 2005-02-05 2005-02-05 Mutanten des fluoreszierenden Proteins CGFPs, sowie deren Verwendung
DE102005005438.2 2005-02-05

Publications (1)

Publication Number Publication Date
WO2006081976A1 true WO2006081976A1 (fr) 2006-08-10

Family

ID=36128319

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/000669 WO2006081976A1 (fr) 2005-02-05 2006-01-26 Mutants de la proteine fluorescente cgfp et leur utilisation

Country Status (3)

Country Link
DE (1) DE102005005438A1 (fr)
TW (1) TW200640945A (fr)
WO (1) WO2006081976A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007140983A1 (fr) * 2006-06-07 2007-12-13 Bayer Healthcare Ag PROTÉINES FLUORESCENTES wfCGFP ET LEUR UTILISATION

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO2000008054A1 (fr) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Proteine a fluorescence verte modifiee
WO2002077011A2 (fr) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Variants proteiques vert fluorescent, sensibles a l'oxydoreduction
WO2004052926A1 (fr) * 2002-12-09 2004-06-24 Bayer Healthcare Ag Protéine fluorescente de clytia gregaria (cgfp) isolée et son utilisation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO2000008054A1 (fr) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Proteine a fluorescence verte modifiee
WO2002077011A2 (fr) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Variants proteiques vert fluorescent, sensibles a l'oxydoreduction
WO2004052926A1 (fr) * 2002-12-09 2004-06-24 Bayer Healthcare Ag Protéine fluorescente de clytia gregaria (cgfp) isolée et son utilisation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CORMACK B P ET AL: "FACS-OPTIMIZED MUTANTS OF THE GREEN FLUORESCENT PROTEIN (GFP)", GENE, ELSEVIER, AMSTERDAM, NL, vol. 173, 1996, pages 33 - 38, XP002919305, ISSN: 0378-1119 *
DELAGRAVE S ET AL: "RED-SHIFTED EXCITATION MUTANTS OF THE GREEN FLUORESCENT PROTEIN", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 13, 1 February 1995 (1995-02-01), pages 151 - 154, XP000574465, ISSN: 0733-222X *
EHRIG T ET AL: "GREEN-FLUORESCENT PROTEIN MUTANTS WITH ALTERED FLUORENCE EXCITATION SPECTRA", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 367, 19 June 1995 (1995-06-19), pages 163 - 166, XP000579119, ISSN: 0014-5793 *
HEIM R ET AL: "ENGINEERING GREEN FLUORESCENT PROTEIN FOR IMPROVED BRIGHTNESS, LONGER WAVELENGTHS AND FLUROESCENCE RESONANCE ENERGY TRANSFER", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 6, no. 2, 1 February 1996 (1996-02-01), pages 178 - 182, XP000676582, ISSN: 0960-9822 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007140983A1 (fr) * 2006-06-07 2007-12-13 Bayer Healthcare Ag PROTÉINES FLUORESCENTES wfCGFP ET LEUR UTILISATION

Also Published As

Publication number Publication date
TW200640945A (en) 2006-12-01
DE102005005438A1 (de) 2006-08-24

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