WO2006080824A1

WO2006080824A1 - Method for identifying object by digitally conversion and extraction of peculiar character through dna typing

Info

Publication number: WO2006080824A1
Application number: PCT/KR2006/000331
Authority: WO
Inventors: Wha-Cheol Jang
Original assignee: Bitgene Co., Ltd
Priority date: 2005-01-31
Filing date: 2006-01-31
Publication date: 2006-08-03
Also published as: KR20060087779A

Abstract

A method for identifying an object by extracting a peculiar character through DNA typing and digitally converting the extracted character is provided. DNA of a living body is extracted and converted into a unique serial character, from which a peculiar character is extracted through the DNA typing which facilitates identifying of a living body and an object kinship, and then digitally converted to thereby authenticate a living body.

Description

DESCRIPTION

METHOD FOR IDENTIFYING OBJECT BY DIGITALLY CONVERSION AND EXTRACTION OF PECULIAR CHARACTER

THROUGH DNA TYPING

TECHNICAL FIELD

The present invention relates to a method for identifying an obj ect by extracting a peculiar character through deoxyribonucleic acid ( DNA) typing and digitally converting the character and, more particularly, to a method in which DNA of a human body is extracted and converted into a unique serial character, from which a peculiar character is extracted through the DNA typing which facilitates identifying of a living body and an obj ect kinship, and then digitally converted to thereby authenticate a living body .

BACKGROUND ART

A typical living body identifying and obj ect kinship identifying method uses short tandem repeat ( STR) makers , which are most commonly used, to identify an individual by analyzing DNA polymorphism of each individual and also identify family members and kinship based on the identified individual . In DNA typing, STR makers include two alleles , namely, allele (p) and allele (q) , in which four bases are repeated, respectively . The two alleles , one of which comes from a paternal line and the other comes from a maternal line, are checked through sequencing .

However, the typical identification method merely analyzes and enumerates the base sequences , failing to create a peculiar character for DNA, digitize the character, and automatically compare, determine and analyze the digital character to identify an individual and a biological child .

DISCLOSURE OF THE INVENTION

TECHNICAL PROBLEM

Therefore, in order to solve the problem of the related art , an obj ect of the present invention is to provide a method for identifying an individual and a biological child in which DNA makers are used for DNA typing, a peculiar serial character data is generated for

DNA by using STR makers and stored, and the serial character data is compared as a reference with that of a different DNA to identify an individual and a biological child .

TECHNICAL SOLUTION

To achieve the above obj ect , there is provided a method for identifying an obj ect by extracting a peculiar character through DNA typing and digitally converting the extracted character, comprising : extracting DNA of a father, a mother and a child by using a sequencing equipment ; setting the order of STR makers of the extracted DNA pertained to the father, the mother and the child; converting the repeat numbers of the STR makers set in the certain order the order with respect to the father, the mother and the child into peculiar digital numbers by using a developed program and storing the digital numbers in a DB of an obj ect server; and comparing the digital numbers of the father, the mother and the child stored in the DB of the obj ect server, and performing determining .

DESCRIPTION OF DRAWINGS FIG . 1 is a simplified configuration diagram showing the construction of a system for identifying an obj ect by extracting a peculiar character through DNA typing and digitally converting the character in accordance with an exemplary embodiment of the present invention . FIG . 2 is a flow chart illustrating the processes of identifying a biological child , among the digital obj ect identifying processes using DNA typing, in accordance with an exemplary embodiment of the present invention .

FIG . 3 is an exemplary diagram showing the number of repeats of base sequences of STR makers pertained to a father, a mother and a child in accordance with an exemplary embodiment of the present invention .

FIG . 4 shows exemplary digital obj ect identifying serial numbers constructed through DNA typing in accordance with an exemplary embodiment of the present invention .

BEST MODE

Exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings .

FIG . 1 is a simplified configuration diagram showing the construction of a system for identifying an obj ect by extracting a peculiar character through DNA typing and digitally converting the character in accordance with an exemplary embodiment of the present invention . The system which includes a manager client 100 , an obj ect identifying server 200 and a member client 300. In detail , the manager client 100 selects a portion where four bases are repeated in STR makers having a gene coming from a paternal line and a gene coming from a maternal line when DNA typing is performed, constructs numerical data through a program developed for generating the number of repeats as to how many times the four bases are repeated as a peculiar character ( including letters of every country and Arabic numbers ) , and transmits the numerical data to the obj ect identifying server 200.

The obj ect identifying server 200 includes a database ( DB) and an obj ect identifying tool (a program developed in filing a patent application) . The DB is constructed by collecting information such as a genotype of a person, a digital character for identifying an individual, a name (gender) , birth of date, nationality ( including an area) , and a gene storage date . The obj ect identifying tool converts the repeat numbers of the STR makers into numerical values , generates a serial character by combining the numerical values , and compares a comparison target obj ect with the serial character to thereby identify an individual and kinship .

The member client 300 requests identifying of an individual and kinship and receives corresponding results .

FIG . 2 is a flow chart illustrating the processes of identifying a biological child by using a digital obj ect identifying system based on DNA typing described in FIG . 1. STR makers used for identifying a biological child includes two alleles (allele (p) and allele (q) ) . In order to determine a biological child, it is checked how many times the four bases of the STR makers are repeated. The repeat numbers are then converted into numerical values by using an obj ect identifying program. Thereafter, the numerical values of the paternal line, the maternal line and a child are compared with each other . If the numerical values are identical by more than a certain percentage, the child is determined as a biological child.

The algorithm for determining the biological child and the constructed program using the same includes the following steps : extracting DNA of the father, the mother and the child by using a sequencing equipment ( step SlO ) ; setting the order of the STR makers of the extracted DNA of the father, the mother and the child ( step S20 ) ; converting the repeat numbers of the STR makers of the father, the mother and the child set in the certain order into digital numbers by using the obj ect identifying program which generates a peculiar character, and storing the digital numbers into the DB of the obj ect server ( step S30 ) ; and comparing the digital characters of the father, the mother and the child stored in the DB of the obj ect server with each other and performing determining ( step S40 ) . In the step SlO of extracting DNA of the father, the mother and the child by using the sequencing equipment , a cell is extracted by using a blood, an oral epithelial tissue, a hair root, a cell-extractable patch and then amplified to extract DNA with a polymerase chain reaction ( PCR) . In the step S20 in which the order of the STR makers of the extracted DNA of the father, the mother and the child is set, every living body has chromosomes . The human body has 23 pairs of chromosomes , and since the chromosomes are formed in pairs , there are 46 chromosomes in total . There exist STR makers allowing identifying of an individual and a biological child, and currently, 17 STR makers are known .

The number of repeats of STR makers is different depending on each individual, and the repeat number of times of each STR maker is different .

Of the STR makers , five STR makers are selectively shown as follows for explanation .

1. D5S818 allele (p) : 7-15 ( 7 , 8 , 9 , 10 , 11 , 12 , 13, 14 , 15 ) allele (q) : 7-15 ( 7 , 8 , 9, 10 , 11 , 12 , 13 , 14 , 15 )

2. D7S820 allele (p) : 6-14 ( 6, 7 , 8 , 9, 10 , 11 , 12 , 13 , 14 ) allele (q) : 6-14 ( 6, 7 , 8 , 9, 10 , 11 , 12 , 13 , 14 )

3. D13S317 allele (p) : 7-15 ( 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ) allele (q) : 7-15 ( 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 )

4. CSFlPO allele (p) : 6-15 ( 6, 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ) allele (q) : 6-15 ( 6, 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 )

5. TOPX allele (P) : 6-14 ( 6, 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ) allele (q) : 6-14 ( 6, 7 , 8 , 9, 10 , 11 , 12 , 13 , 14 )

Of the STR makers, a portion where four bases are repeated is checked. For instance, the number of repeats of ATTT , the most common repeat base sequences , is checked, and the order of the STR markers is to obtain a unique serial character from a peculiar character, which is digitized by using the program that generates the number of repeats into the peculiar character, to thereby set the order of the plurality of STR makers . The order of the STR makers can be freely changed, but the order of the STR makers of the father, the mother and the child should be the same so as to be read as digital values for identifying a biological child.

In the step S30 in which the repeat numbers of the

STR makers of the father, the mother and the child set in the certain order are converted into digital numbers in the set order by using the obj ect identifying program which generates a peculiar character and then stored in the DB of the obj ect server, the order of the STR makers is set in the step S20 , and as shown in FIG . 3A, the repeat numbers of the base sequences of the STR makers , starting from the first STR maker up to the seventeenth STR maker, pertained to the father, the mother and the child is digitally converted. In detail, the repeat number of the ATTT, the most common repeated base sequences in DNA printing, is digitized . In more detail, the father STR maker 10 of ' A' in FIG . 3 is an numerical embodiment of the repeat numbers

( 11 , 12 ) from the paternal line, allele (p) , and the maternal line , allele (q) , respectively. In this manner, the first STR maker to the seventeenth STR maker are all turned into characters as digital values .

In FIG . 3 , regarding ' B ' and ¹ C , the repeat numbers of the mother and the child are digitized substantially in the same manner as in the case of ¹ A' , the numbers for allele (p) and allele (q) numbers of the STR makers are extracted by the PCR equipment, which are then converted into digital characters by using the obj ect identifying program. The obtained number is unique and sole in the whole world, namely, a unique serial character, which can be used as a reference for authenticating and identifying an individual .

As shown in FIG . 4 , the order 40 of the STR makers is set , an STR maker 41 is analyzed by using the PCR equipment, and digital numbers 42 of allele (p) and allele ( q) are arranged . Then, a peculiar character 1101091201023232010222010304 can be generated. The peculiar character becomes the unique serial character, which allows identifying of an individual with an intended level of accuracy . In addition, if the unique serial character is considered too long to be used for a social security number, a foreigner registration number or any other identification substitutes , a calculation program, which performs a series of arithmetic operation, is employed to reduce the unit number of the long serial character obtained by combining allele (p) and allele (q) . This reduced unit number can be reduced by using a calculation program which performs a series of arithmetical operation so as to be used as a personal peculiar character such as a social security number .

In the step S40 for comparing and determining the digital characters of the father, the mother and the child stored in the obj ect server DB, the base sequence repeat numbers are converted into the digital numbers , which are then compared and read . This step will be explained together with ' A ' and ' C in FIG . 3. Since allele (p) extracted from the certain STR maker is a gene coming from the paternal line and allele (q) is a gene coming from the maternal line, the repeat numbers of allele (p) and allele (q) of the paternal line in ' A¹ of FIG . 3 isare 7 and 10 , the repeat numbers of allele (p) and allele (q) of the maternal line in ¹ C of FIG . 3 are 8 and 10 , and the repeat numbers of allele (p) and allele (q) of the child are 7 and 11. In this case, only one of the base sequence repeat numbers of the child is the same as those received from the father or the mother, the obj ect identifying server 200 authenticates this match as an acceptable status , ¹ OK ' . When the second STR makers of the ' A' and ¹ C in FIG . 3 are compared substantially in the same manner, since the base sequence repeat numbers received from the father or the mother is not the same, the obj ect identifying server 200 authenticates this non-match as a denial status , ' NO ' ? After up to the 17th STR maker is read in this manner, when OK-authenticated STR makers exceed by approximately 50% overall , the child is determined as a biological child .

The obj ect identifying server 200 provides the probability of the biological child by using the arithmetic operation program installed therein . In addition, in case where a personal DB is constructed with the serial character, the member client 300 can search an inputted serial character as inputted by the obj ect identifying server 200 and precisely discriminate an individual and a biological child promptly .

A method for identifying an individual includes the same process as the steps SlO to S30 of the biological child identifying process ( steps SlO to S40 ) , and the probability in the step S40 is approximately 100% . This method will be described in detail with reference to FIG . 4.

In order to construct a serial character peculiar to an to an individual , as shown in FIG. 2 , each DNA of a father, a mother and a child is extracted by using the sequencing equipment ( step SlO ) , the order of STR makers of the extracted DNA from the father, the mother and the child is set ( step S20 ) , and the repeat numbers of the STR makers set in a certain order with respect to the father, the mother and the child are converted into digital numbers through the program which generates a peculiar character and then stored into the DB of the obj ect server ( step S30 ) , whereby a peculiar serial character of an individual can be generated and stored as data in the obj ect identifying server 200. By using the serial character data constructed in the obj ect identifying server 200 as reference data, a cell of an unidentified person can be collected and then comparison data can be extracted by performing the steps SlO to S30. Since the reference data and the comparison data include the respective peculiar character for each individual , the obj ect identifying server 200 can simply compare the numerical values of the two data, and if they are identical by approximately 100% , the obj ect identifying server 200 discriminates them as the same person . INDUSTRIAL APPLICABILITY

As described above, the present invention is advantageous as follows . Since a peculiar number is created solely for DNA and stored, an unidentified person can be easily identified by using the peculiar number . Also, the peculiar number can be used as a substitute for the current social ID card formed in a card type or laminated paper, etc . In addition, a basic database for specially managing criminals and people with contagious diseases , or the like , can be constructed and utilized . Moreover, the peculiar digital character generated through the obj ect identifying program can be stored in a barcode, a 2D code, color code or an RFID chip and embedded in a credit card for double authentication . Consequently, the present invention is useful in terms of biotechnology and information technology.

Claims

1. A method for identifying an object by extracting a peculiar character through DNA typing and digitally converting the extracted character, comprising : extracting each DNA of a father, a mother and a child by using a sequencing equipment (SlO ) ; setting the order of STR makers of the extracted DNA of the father, the mother and the child ( S20 ) ; converting the repeat numbers of the STR makers of the father, the mother and the child into respective peculiar digital numbers according to the set order by using an obj ect identifying program which generates a peculiar character, and storing the peculiar digital numbers into a DB of an obj ect server ( S30 ) ; and comparing the digital numbers of the father, the mother and the child stored in the DB of the obj ect server, and performing determining (S40) .

2. The method of claim 1 , wherein the digital character includes a reference data with a serial character peculiar to an each individual , and when a comparison target data is compared with the reference data and determined to be identical by approximately 100% , the obj ective identifying server 200 determines that the reference data and the comparison target data are originated from the same person .

3. The method of claim 1 or 2 , wherein the digital character is the repeat numbers of allele (p) and allele (q) .

4. The method of claim 1 or 2 , wherein the digital character is a peculiar character generated through the obj ect identifying program and used to digitally identifying an obj ect, and the number of units of the digital character is reduced to a smaller unit by using an arithmetic operation program to thereby identify an individual .