WO2006080032A3 - Antifungal protein - Google Patents

Antifungal protein Download PDF

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Publication number
WO2006080032A3
WO2006080032A3 PCT/IN2006/000035 IN2006000035W WO2006080032A3 WO 2006080032 A3 WO2006080032 A3 WO 2006080032A3 IN 2006000035 W IN2006000035 W IN 2006000035W WO 2006080032 A3 WO2006080032 A3 WO 2006080032A3
Authority
WO
WIPO (PCT)
Prior art keywords
afpl
amino acids
purified
protein
antifungal activity
Prior art date
Application number
PCT/IN2006/000035
Other languages
French (fr)
Other versions
WO2006080032A2 (en
Inventor
Venga Siva Reddy
Afif Hassari
Aparna Isalm
Original Assignee
Internationl Ct For Genetic
Venga Siva Reddy
Afif Hassari
Aparna Isalm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Internationl Ct For Genetic, Venga Siva Reddy, Afif Hassari, Aparna Isalm filed Critical Internationl Ct For Genetic
Publication of WO2006080032A2 publication Critical patent/WO2006080032A2/en
Publication of WO2006080032A3 publication Critical patent/WO2006080032A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

For the first time, a small cystine rich protein (AFPl-Ca) secreted from the germinating seeds of a legume plant, chickpea (Cicer arietinum, L) with antifungal activity has been identified, purified and characterized the encoding gene cloned. The purified protein inhibited the growth of Pythium aphanidermatum mycelium at an IC50 value of 12 µg/ml. The SDS-PAGE analysis indicated the presence of disulfide bridges and a prior treatment of purified AFPl-Ca with a reducing agent, β- mercaptoethanol, abolished the antifungal activity completely. The complete gene coding for the AFPl-Ca was isolated, DNA sequenced and the deduced amino acid sequence revealed that the gene code for (74) amino acids; (27) amino acids of signal peptide and (47) amino acids of mature protein.
PCT/IN2006/000035 2005-01-31 2006-01-31 Antifungal protein WO2006080032A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2617/DEL/2004 2005-01-31
IN2617DE2004 2005-01-31

Publications (2)

Publication Number Publication Date
WO2006080032A2 WO2006080032A2 (en) 2006-08-03
WO2006080032A3 true WO2006080032A3 (en) 2006-12-21

Family

ID=36740904

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2006/000035 WO2006080032A2 (en) 2005-01-31 2006-01-31 Antifungal protein

Country Status (1)

Country Link
WO (1) WO2006080032A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220061333A1 (en) * 2019-01-07 2022-03-03 Donald Danforth Plant Science Center Antimicrobial ncr peptides
US11713341B1 (en) 2022-06-17 2023-08-01 Vestaron Corporation Antimicrobial NCR13 variant peptides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5689043A (en) * 1991-08-29 1997-11-18 Zeneca Limited Biocidal proteins
US6329504B1 (en) * 1996-12-13 2001-12-11 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5689043A (en) * 1991-08-29 1997-11-18 Zeneca Limited Biocidal proteins
US6329504B1 (en) * 1996-12-13 2001-12-11 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 6 September 1990 (1990-09-06), retrieved from EBI Database accession no. X16877 *
HANCOCK R E W ET AL: "Cationic peptides: a new source of antibiotics", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 16, no. 2, February 1998 (1998-02-01), pages 82 - 88, XP004107047, ISSN: 0167-7799 *
ISHIBASHI N ET AL: "STORED MRNA IN COTYLEDONS OF VIGNA UNGUICULATA SEEDS: NUCLEOTIDE SEQUENCE OF CLONED CDNA FOR A STORED MRNA AND INDUCTION OF ITS SYNTHESIS BY PRECOCIOUS GERMINATION", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 15, 1990, pages 59 - 64, XP002024739, ISSN: 0167-4412 *

Also Published As

Publication number Publication date
WO2006080032A2 (en) 2006-08-03

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