WO2006073360A1

WO2006073360A1 - NEW USE OF 11-PIPERAZIN-1-YLDIBENZO [b,f] [1,4] THIAZEPINE OR ITS PHARMACEUTICALLY ACCEPTABLE SALT AND TO ORAL PHARMACEUTICAL COMPOSITIONS

Info

Publication number: WO2006073360A1
Application number: PCT/SE2006/000009
Authority: WO
Inventors: Patricia C. Davis; Jeffrey M. Goldstein; Scott W. Grimm; Raymond F. Suckow; Helen R. Winter
Original assignee: Astrazeneca Ab
Priority date: 2005-01-07
Filing date: 2006-01-04
Publication date: 2006-07-13
Also published as: AR052191A1; EP1838325A1; TW200640466A; JP2008526839A; UY29326A1

Abstract

A method of treating at least one symptom or condition associated with but not limited to: 1) Substance-Related Disorders including but not limited to Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol-Related Disorders, Amphetamine (or Amphetamine-Like)-Related Disorders, Caffeine-Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen-Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related Disorders. 2) Attention-Deficit and Disruptive Behavior Disorders. 3) Eating Disorders. 4) Personality Disorders including but not limited to Obsessive-Compulsive Personality Disorder. 5) Impulse-Control Disorders, comprising administering an effective amount of Formula I (I) or its pharmaceutically acceptable salt. In another aspect of the invention a pharmaceutical composition is provided comprising an effective amount of Formula I or its pharmaceutically acceptable salt and at least one pharmaceutically acceptable carrier or diluent.

Description

New use of ll-piperazin-l-yldibenzo [b, f] [1 , 4] thiazepine or its pharmaceutically acceptable salt and to oral pharmaceutical compositions

FIELD OF THE INVENTION

The present invention provides pharmaceutical compositions and methods relating to 11 -piperazin- 1 -yldibenzo[δ/j [1 ,4]thiazepine. BACKGROUND OF THE INVENTION

A goal of antipsychotic drug development has been to develop agents with increased efficacy and safety along with fewer of the side effects commonly associated with the older antipsychotic medications. Quetiapine fumarate is described in U.S. Patent Number 4,879,288, which is incorporated herein by reference. Quetiapine fumarate is able to treat both the positive (hallucinations, delusions) and negative symptoms (emotional withdrawal, apathy) of psychosis and is associated with fewer neurological and endocrine related side effects compared to older agents. Quetiapine fumarate has also been associated with a reduction in hostility and aggression. Quetiapine fumarate is associated with fewer side effects such as EPS, acute dystonia, acute dyskinesia, as well as tardive dyskinesia. Quetiapine fumarate has also helped to enhance patient compliance with treatment, ability to function and overall quality of life, while reducing recidivism. P. Weiden et al., Atypical antipsychotic drugs and long-term outcome in schizophrenia, 11 J. Clin. Psychiatry, 53-60, 57 (1996). Because of quetiapine fumarate's enhanced tolerability profile its use is particularly advantageous in the treatment of patients that are hypersensitive to the adverse effects of antipsychotics (such as elderly patients).

Derivatives of 1 l-(piperazin-l-yl)dibenzo[b,f][l,4]-thiazepines and related compounds including metabolites of quetiapine were prepared and evaluated in E. Warawa et al. Behavioral approach to nondyskinetic dopamine antagonists: identification ofSeroquel, AA, J. Med. Chem., 372-389 (2001). Quetiapine metabolism has been reported in C. L. Devane et al. Clin. Pharmacokinet., 40(7), 509-522 (2001) wherein the structure of 11-piperazin-l- yldibenzo[b,f][l,4]thiazepine (see Formula I below) was shown in Figure 1. This compound was reported by Schmutz et al. in U.S. Patent No: 3,539,573. This compound has also been used in processes for preparing quetiapine as reported in U.S. Pat. No. 4,879,288. It has now been found that l l-piperazin-l-yldibenzo[b,f][l,4]thiazepine is a circulating metabolite of quetiapine in humans. SUMMARY OF THE INVENTION ll-Piperazin-l-yldibenzo[&/][l,4]thiazepine has the structure as shown by Formula I:

I

Provided herein is a method of treating, comprising the administration of an effective amount of Formula I or its pharmaceutically acceptable salt to a mammal, at least one symptom or condition associated with, but not limited to: 1) Sleep Disorders including but not limited to a)Dyssomnia Disorders including but not limited to: i) Primary inomnia, ii) Primary Hypersomnia, and iii) narcolepsy; and b) Parasomnias Disorders including but not limited to i) Nightmare Disorder, Sleep Terror Disorder and Sleepwalking Disorder. Disorders Usually First Diagnosed in Infancy, Childhood, or Adolescence including but not limited to Mental Retardation, Learning Disorders, Motor Skills Disorder, Communication Disoorders, Pervasive Developmental Disorders, Attention-Deficit and Disruptive Behavior Disorders, Feeding and Eating Disorders of Infancy or Early Childhood, Tic Disorders, and Elimination Disorders; 2) Substance-Related Disorders including but not limited to Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol- Related Disorders, Amphetamine (or Amphetamine-Like)-Related Disorders, Caffeine- Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen- Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related Disorders; 3) Attention-Deficit and Disruptive Behavior Disorders; 4) Eating Disorders; 5) Personality Disorders including but not limited to Obsessive-Compulsive Personality Disorder; and 6) Impulse-Control Disorders.

Examples of definitions of the above conditions and disorders can be found, for example, in the American Psychiatric Association: Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision, Washington, DC, American Psychiatric Association, 2000. Substance abuse and substance dependence and related disorders are also defined therein. This Manual may also be referred to for greater detail on the symptoms and diagnostic features associated with substance use, abuse and dependence. Typical substances that lead to substance abuse and substance dependence include drags such as amphetamines, cannabis, cocaine, crack, hallucinogenic agents, inhalants, opioids, phencyclidine, sedatives, hypnotics, anxiolytic agents and alcohol. Nicotine can also lead to substance dependence.

In another aspect of the invention provided is a pharmaceutical composition comprising an effective amount of the compound of Formula I or its pharmaceutically acceptable salt and at least one pharmaceutically acceptable carrier. Also provided is a method of treating the symptoms or conditions provided herein comprising administering to a mammal a pharmaceutical composition as described above. Also provided is the use of the compound of Formula I or its pharmaceutically acceptable salt and/or the above-mentioned pharmaceutical composition in the treatment of the symptoms or conditions provided herein in mammals. Also provided is the use of the compound of Formula I or its pharmaceutically acceptable salt administered in combination with one or more other therapeutically active agents. Further, provided herein is the use of the compound of Formula I or its pharmaceutically acceptable salt and/or the pharmaceutical composition in the manufacture of a medicament for use in the treatment of the symptoms or conditions provided herein in mammals. BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 depicts an X-ray powder diffraction (XRPD) pattern consistent with crystalline 1 l-piperazin-l-yldibenzo[£/][l,4]thiazepine having Form A. DETAILED DESCRIPTION OF THE INVENTION

The compound of Formula I is a dibenzothiazepine that has shown antidopaminergic activity. It has been shown to interact with a broad range of neurotransmitter receptors but has a higher affinity for serotonin (5-HT₂) receptors relative to dopamine (D₂) receptors in the brain. Preliminary positron emission topography (PET) scans of primate subjects indicated that the compound of Formula I reaches the brain and occupies Dl, D₂, 5-HT_2A, and 5-HT₁A receptors and the 5HT Transporter. However, the compound of Formula I was not shown to be efficacious in a mouse standard apomorphine swim test (p.o.) and in a rat D-Arnpehtamine locomotor activity test (s.c).

The compound of Formula I has also been shown to have partial 5HT_1A agonist activity and has shown in-vivo efficacy in mouse and rat models for depression. The compound of Formula I may be used as an antipsychotic with a reduction in the potential to cause side effects such as acute dystonia, acute dyskinesia, as well as tardive dyskinesia typically seen with antipsychotics. Results generated from alpha receptor binding data further suggest that the compound of Formula I will have improved tolerability over that of quetiapine and suggest that one would observe a reduced incidence of hypotension. Further the compound of Formula I may be used to treat patients of all ages and is advantageous in the treatment of elderly patients.

The term "mammal" means a warm-blooded animal, preferably a human.

The compound of Formula I may be made by a variety of methods known in the chemical arts. The compound of Formula I may be prepared by starting from known compounds or readily prepared intermediates including taking the lactam of Formula II:

II which may be prepared by methods well known in the literature, for example, as described by J. Schmutz et al. HeIv. Chim. Acta., 48:336 (1965). The lactam of Formula II is treated with phosphorus chloride to generate the immino chloride of Formula III:

III

The immino chloride of Formula III may also be generated with other agents such as thionyl chloride or phosphorous pentachloride. The imino chloride is then reacted with piperazine to give the compound of Formula I.

The compound of Formula I provided herein is useful as a free base, but may also be provided in the form of a pharmaceutically acceptable salt, and/or in the form of a pharmaceutically acceptable solvate (including hydrates). For example, pharmaceutically acceptable salts of Formula I include those derived from mineral acids such as for example: hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, hydroiodic acid, nitrous acid, and phosphorous acid. Pharmaceutically acceptable salts may also be developed with organic acids including aliphatic mono dicarboxylates and aromatic acids. Other pharmaceutically acceptable salts of Formula I include but are not limited to hydrochloride, sulfate, pyrosulfate, bisulfate, bisulfite, nitrate, and phosphate.

A clinician may determine the effective amount by using numerous methods already known in the art. The term "treating" within the context of the present invention encompasses to administer an effective amount of the compound of the present invention, to mitigate either a pre-existing disease state, acute or chronic, or a recurring symptom or condition. This definition also encompasses prophylactic therapies for prevention of recurring conditions and continued therapy for chronic disorders.

A particular amount of the compound of Formula I or its pharmaceutically acceptable salt can be administered in an amount up to about 750 mg per day; particularly from about 75 mg to about 750 mg per day. In another particular aspect of the invention the amount of the compound of Formula I₅ or its pharmaceutically acceptable salt, may be administered from about 1 mg to about 600 mg per day. In another aspect of the invention the compound of Formula I or its pharmaceutically acceptable salt may be administered from about 100 mg to about 400 mg per day.

The compound of Formula I or its pharmaceutically acceptable salt may be administered comprising a predetermined dosage of the compound of Formula I to a mammal between one and four times a day, wherein the predetermined dosage is from about 1 mg to about 600 mg.

The present invention also provides a method of treating the symptoms or conditions provided herein comprising the step of administering an initial predetermined dosage of a compound of Formula I to a human patient twice a day, wherein the predetermined dosage is between 1 mg and 30 mg with increases in increments of 1-50 mg twice daily on the second and third day as tolerated. Thereafter, further dosage adjustments can be made at intervals of no less than 2 days.

In one embodiment of the invention the pharmaceutical composition comprises up to about 750 mg of the compound of Formula I or its pharmaceutically acceptable salt, particularly from about 75 mg to about 750 mg.

In another embodiment of the invention, the pharmaceutical composition may comprise from about 1 mg to about 600 mg of the compound of Formula I or a pharmaceutically acceptable salt thereof. In another embodiment of the invention, the pharmaceutical composition may comprise from about 100 mg to about 400 mg of the compound of Formula I or a pharmaceutically acceptable salt thereof.

The pharmaceutical composition of the invention may accordingly be obtained by conventional procedures using conventional pharmaceutical excipients. Thus, pharmaceutical compositions intended for oral use may contain, for example, one or more coloring, sweetening, flavoring and/or preservative agents.

For preparing pharmaceutical compositions from the compound of Formula I of this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets, and suppositories.

The composition of the invention may be administered by any route including orally, intramuscularly, subcutaneously, topically, intranasally, intraperitoneally, intrathoracially, intravenously, epidurally, intrathecally, intracerebroventricularly and by injection into the joints.

The amount of active ingredient that is combined with one or more excipients to produce a single dosage form, such as an oral dosage form, will necessarily vary depending upon the host treated and the particular route of administration. The size of the dose for therapeutic or prophylactic purposes of a compound of the Formula I will naturally vary according to the nature and severity of the symptoms or conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine.

Another aspect of the invention provides a compound of Formula I, or its pharmaceutically acceptable salt or solvate thereof, for use in treating the symptoms or conditions provided herein.

In a further aspect, the present invention provides the use of a compound of Formula I, or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for use in treating the symptoms or conditions provided herein.

In a further aspect, the present invention relates to methods of treating at least one of the above described symptoms or conditions comprising administering to a mammal an effective amount of the compound of Formula I or its pharmaceutically acceptable salt and one or more of other therapeutically active agents, benzodiazepines, 5-HTJ_A ligands, 5-HT_1B ligands, 5-HT_1D ligands, mGluR2A agonists, mGluR5 antagonists, antipsychotics, NKl receptor antagonists, antidepressants, serotonin reuptake inhibitors, GABA II ligands, or mood stabilizers administered in combination as part of the same pharmaceutical composition, as well as to methods in which such active agents are administered separately as part of an appropriate dose regimen designed to obtain the benefits of combination therapy. The appropriate dose regimen, the amount of each dose of an active agent administered, and the specific intervals between doses of each active agent will depend upon the subject being treated, the specific active agent being administered and the nature and severity of the specific disorder or condition being treated. In general, the compounds of this invention, when used as either a single active agent or when used in combination with another active agent, will be administered to a subject in an amount up to about 750 mg per day, in single or divided doses. Such compounds may be administered on a regimen of up to 6 times per day, preferably 1 to 4 times pe/ day. Variations may nevertheless occur depending upon the subject being treated and the individual response to the treatment, as well as on the type of pharmaceutical formulation chosen and the time period and interval at which such administration is carried out. In some instances, dosage levels below the lower limit of the aforesaid range may be more than adequate, while in other cases larger doses may be employed to achieve the desired effect, provided that such larger doses are first divided into several small doses for administration throughout the day.

Exemplary benzodiazepines may include but are not limited to adinazolam, alprazolam, bromazepam, clonazepam, chlorazepate, chlordiazepoxide, diazepam, estazolam, flurazepam, balezepam, lorazepam, midazolam, nitrazepam, oxazepam, quazepam, temazepam, triazolam and equivalents thereof.

Exemplary 5-HT_1A and/or 5HT_1B ligands may include but are not limited to buspirone, amespirone, elzasonan, ipsapirone, gepirone, zopiclone and equivalents thereof.

Exemplary mGluR 2 agonists may include (lS,3R)-l-aminocyclopentane-l,3- dicarboxylic acid, (2S,3S,4S)alpha-(carboxycyclopropyl)glycine, and 3,5- dihydroxyphenylglycine.

Exemplary antidepressants may include but are not limited to maprotiline, amitriptyline, clomipramine, desipramine, doxepin, imipramine, nortryptyline, protriptyline, trimipramine, SSRIs and SNRIs such as fluoxetine, paroxetine, citalopram, escitalopram, sertraline, venlafaxine, fluoxamine, and reboxetine. Exemplary antipsychotics may include but are not limited to clozapine, risperidone, quetiapine, olanzapine, amisulpride, sulpiride, zotepine, chlorpromazine, haloperidol, ziprasidone, and sertindole.

Exemplary mood stabilizers may include but are not limited to Valproic acid (valproate) and its derivative (e.g. divalproex), lamotrigine, lithium, verapamil, carbamazepine and gabapentin.

The following examples provided are not meant to limit the invention in any manner and are intended for illustrative purposes only. EXAMPLES Example 1 Preparation of 1 l-piperazin-l-yldibenzor&,/iri,4^~lthiazepine

Into a 1000 mL round-bottom flask equipped with a magnetic stirring bar and reflux condenser with a nitrogen inlet was charged with 25.0 grams (g) (0.110 mole) of dibenzo[b,fj[l,4]thiazepine-l l(10-H)-one (made by the method disclosed by J. Schmutz et al. HeIv. Chim. Acta., 48: 336 (1965)), as a dry solid, followed by 310 mL POCl₃ and 3 mL of N,N-dimethylaniline. The reaction mixture was heated at reflux (106 degrees C) for 6 hours giving a clear orange solution. The reaction was then cooled to room temperature, and POCl₃ removed on the rotary evaporator leaving an orange oil. This residue was partitioned between ice -water (500 mL) and ethyl acetate (800 mL). The layers were separated and the aqueous phase extracted with ethyl acetate (3 X 200 mL). The combined ethyl acetate extracts were dried over MgSO₄ , filtered, and then stripped down on the rotary evaporator, leaving the crude imino chloride as a light yellow solid (26.26g, 97% yield). The structure was confirmed by NMR and Mass Spectrum (300 MHz, CDCl₃; ES+, M+l = 246.7). Crude imino chloride (27.35 g, 0.111 mole) was added to 1000 mL o-xylene in a 2000 mL round-bottom flask equipped with a magnetic stir bar and a reflux condenser with nitrogen inlet. To this solution was added commercially available piperazine (47.95g, 0.557 mole) in one portion as a dry solid at room temperature. The mixture was stirred until nearly all the piperazine dissolved. Then the reaction mixture was heated at reflux (142 degrees C) for 40 hours (out of convenience). The reaction was then allowed to cool to room temperature, and an aliquot was partitioned between IN NaOH / CH₂Cl₂. The organic phase was checked by TLC (silica gel, CH₂Cl₂ / Methanol 90:10, iodoplatinate visualized) and showed clean conversion to one major product (Rf = 0.45). A drop of the reaction solution was diluted with CH₃CN to prepare a sample for LC / MS analysis, which confirmed the presence of the desired product (M+l = 296.4). The reaction mixture was stripped down on the rotary evaporator under high vacuum to remove the xylene. The residue was partitioned between IN NaOH (400 mL) and CH₂Cl₂ (200 mL). The layers were separated, and the aqueous phase further extracted with CH₂Cl₂ (3 X 200 mL). The combined CH₂Cl₂ extracts were washed with brine (200 mL), then dried over MgSO₄, filtered, and stripped down on the rotary evaporator to give the crude title compound as a yellow gum (35.3 g). The crude free base was purified by flash column chromatography over silica gel (600 g) eluting with a gradient of 0 to 20% Methanol in CH₂Cl₂. Fractions containing the pure desired product were combined and stripped down on the rotary evaporator, to afford the purified free base as a light yellow foam (25.67g, 78% yield). Example 2 Preparation of l l-piperazin-l-yldibenzorfr₇firi,4]thiazepine, dihydrochloride salt

The free base was converted to it's dihydrochloride salt by dissolving it in a mixture of methanol (125 mL) and diethyl ether (125 mL), then treating with 250 mL of 1.0 M HCl/ ether (Aldrich). An off -white gummy solid separated initially, and the mixture was farther diluted with 50Q mL ether. The gummy solid did not solidify on prolonged stirring. The solvents were decanted away from the gum. The gum was treated with absolute ethanol (200 mL), then stirred until crystallization occurred, giving a thick white suspension of crystals. This mixture was then slowly diluted with ether (800 mL) and allowed to stir overnight to complete the crystallization. The crystalline dihydrochloride salt was isolated by filtration, washed with ether (3 X 50 mL), then dried in vacuum at 60 degrees C to afford the dihydrochloride salt of the title compound as a white crystalline solid (31.64 g, 98.8% conversion). ANALYSIS:

The product was characterized by NMR and LC / MS (300MHz, CDCl₃; AP+, M+l = 296.4). Example 3

Preparation of crystalline 11 -τήperazin-1 -yldibenzo|^"b,f] [1 Λlthiazepine^') Preparation A

Aqueous solution (584 mL; e.g., prepared by extraction of 11-piperazin-l- yldibenzo[b,fj[l,4]thiazepine into water/HCl from a toluene solution such as described below in Preparation B) containing 1 l-piperazin-l-yldibenzo[b,fj[l,4]thiazepine hydrochloride was charged to a jacketed 1 L flask. The flask was then charged with toluene (500 mL) and sodium hydroxide (48% w/w, 33.0 g). The mixture was stirred at 70 ⁰C for 30 minutes and became white and cloudy. The mixture was then allowed to settle for 30 min and the phases were separated. The toluene layer was washed at 70 ⁰C with 2x100 mL of water (1^st wash = pH 10.3; 2^nd wash = pH 8.0). The final toluene volume was 560 mL containing about 74 g of 1 l-piperazin-l-yldibenzo[b,fj[l,4]thiazepine in good purity.

The above procedure was repeated for an additional four aqueous solutions of 11- piperazin-l-yldibenzo[b,f][l,4]thiazepine hydrochloride and the five resulting toluene solutions were combined and evaporated to dryness on a rotary evaporator. The resulting hard solid was then charged to a jacketed vessel and slurried with methyl-t-butyl ether (MTBE) (500 mL). The resulting slurry was stirred overnight at ambient temperature and then cooled to 5 ⁰C and held for 4 h. The solid 1 l-piperazin-l-yldibenzo[b,fj[l,4]thiazepine product was isolated on a no. 3 sinter and washed with 200 mL of cold MTBE. The cake was dried in a vacuum oven overnight at 60 ⁰C yielding 373 g of product. Preparation B

A toluene solution of 1 l-piperazin-l-yldibenzo[b,fj[l,4]thiazepine (1500 mL, 0.686 mol) prepared by reaction of piperazine with 1 l-chloro-dibenzo[b,fj[l,4]-thiazepine in toluene (see, e.g., U. S. Pat. No. 4,879,288) was treated with 1500 mL deionized water and 90 mL of HCl (32% w/w). The resulting mixture was heated to 70 ⁰C and agitated for 45 min. Agitation was ceased and the mixture allowed to settle and phase separate for 30 min. The lower aqueous phase, containing the HCl salt of 1 l-piperazin-l-yldibenzo[b,fj[l₅4]thiazepine was isolated. The aqueous phase was then treated with 1000 mL of toluene and 99 g of aqueous NaOH (47% w/w). The resulting mixture was heated to 70 °C and agitated for 45 min. Agitation was ceased and the mixture allowed to settle and phase separate for 30 min. The lower aqueous phase was discarded and the upper organic phase retained to which 300 mL of deionized water was added. The resulting mixture was agitated for 15 min and then allowed to settle for 30 min. The aqueous phase was discarded and the organic phase retained. The organic phase was extracted once more with 300 mL of deionized water. About 750 mL of toluene from the organic phase was distilled out. The resulting concentrate was cooled to 60 °C, then 200 mL of methyl-t-butyl ether (MTBE) was added. The resulting mixture was cooled to ambient temperature then seeded with Form A seed crystals. The seeded mixture was then cooled to 10 °C and held at this temperature for 3 hours under slow agitation. The resulting solid was isolated under suction via a no. 3 sinter. The solid product was then washed with 120 mL of MTBE at ambient temperature and dried at 40 °C under vacuum resulting in 175 g (86.4%) of crystalline product. Assay 99.9% w/w by HPLC area %.

Solid l l-piperazin-l-yldibenzo[b,fj[l,4]thiazepine (30 g, 0.1016 mol) prepared as described above was slurried in isopropanol (120 mL). The resulting mixture was warmed to about 63-64 °C to completely dissolve the solid. The resulting solution was filtered through a preheated (about 55 °C) split Buchner funnel fitted with filter paper with a pore size of 6 μm. ^' The filtered solution was then adjusted to 55 °C and seeded with seed crystals of Form^' A (0.024 g). The seeded solution was maintained at 55 °C for about 2 h then linearly cooled to 40 ⁰C over the course of 6 h, linearly cooled to 20 ⁰C over the course of 2 h, and then linearly cooled to 0 °C over the course of 1 h. The resulting slurry was held at 0 °C for 12 h and the solid product cake (13 mm high x 68 mm diameter) was isolated by filtration. The product cake was displacement washed with 30 mL isopropanol prechilled to 0 °C and the cake allowed to deliquor. The product was then dried at 40 °C under vacuum yielding 24.9 g (83%) of Form A. Assay by NMR: 98.9% w/w.

X-ray powder diffraction (XRPD) peak data of crystalline Form A is provided below in Chart A. The following instrument setting were used.

Instrument Bruker D8 Discover

Scan range 2-40° 2Θ

Step size 0.007° 2Θ

Scan speed 0.2 sec/step

Scan type 2TH/T

Lamp intensity 35kV/45 mA Chart A (Form A) Angle Intensity Intensity

2-Theta ° Count %

10.8 18321 51.4

12.3 2390 6.7

13.3 24555 68.9

15.2 12193 34.2

15.3 9799 27.5

16.0 2414 6.8

17.2 18803 52.7

18.8 6502 18.2

19.3 7290 20.4

20.0 3666 10.3

20.4 15535 43.6

21.2 ^' 25874 72.6

21.7 16902 47.4

22.1 1473 4.1

24.1 3968 11.1

24.2 21.97 . 6.2

24.9 3579 10

25.5 35663 100

26.4 6298 17.7

27.9 3290 9.2

28.0 3746 10.5

28.3 2206 6.2

28.6 2711 7.6

28.9 2142 6

29.4 4006 11.2

29.8 2464 6.9

30.4 2754 7.7

30.9 5213 14.6

31.0 5143 14.4

31.6 2053 5.8

32.1 3643 10.2

32.4 4234 11.9

32.5 3827 10.7

33.2 2102 5.9 34.6 1540 4.3

35.8 1543 4.3

36.3 3768 10.6

36.9 3086 8.7

38.1 2062 5.8

39.0 2801 7.9

39.4 1492 4.2

Example 4

In-vitro binding data for brain serotonin 5-HT?, Dl and D2 receptors and in-vivo rat and mouse studies.

An assessment of dopamine antagonism was made in rodent models. The methods and procedures αised can be found in J. Med. Chem., 44 (3), 372 -389, 2001 and are incorporated herein by reference. The results are as follows the binding affinity for brain serotonin 5-HT₂ receptor was 27 K₁ nM, and for dopamine Di and D₂ receptors was 1489 and 234 Ki nM,^' respectively. These results show that the compound of the present invention as the dihydfochloride salt interacts with a broad range of neurotransmitter receptors, however, the assay also reveals that that the compound of the present invention as the dihydrochlδfide salt has a higher affinity for serotonin (5-HT₂) receptors relative to dopamine (D₂) receptors in the brain. It is this combination of serotonin and dopamine receptor antagonism, with higher relative 5-HT₂ to D₂ receptor affinity that indicates that the compound of Formula I or its pharmaceutically acceptable salts would act as a potent atypical antipsychotic. J. Goldstein, Quetiapine Fumarate (Seroquel): a new atypical antipsychotic, 35(3) Drugs of Today 193- 210 (1999).

However, in vivo antipsychotic activity of 11-piperazin-l- yldibenzo[b,fj[l,4]thiazepine dihydrochloride was tested in mice (p.o) according to a standard apomorphine climbing mouse assay and in an rats (freebase) in a D-amphetamine locomotor activity test and the compound was not found to be efficacious in these models-

Example 5 αl and α2 receptor profile

Differentiation of l l-piperazin-l-yldibenzo[b/|[l,4]thiazepine from quetiapine is based on alpha receptor binding data shown below. Receptor Quetiapine Affinity (nM) (I) Affinity (nM) αlA 22 108 αlB 39 75 αlD — 185 α2C 28 820

The above affinity values were derived from the below results, methods and criteria.

PRIMARY

SPECIES CONC. % INH. ICso la n BIOCHEMICAL ASSAY

Adrenergic (X_IA rat 0.3 μM 51 0.268 ± 0.012 μM 0.108 ± 0.005 μM 1.03 ± 0.089

Adrenergic (X_IB rat 0.1 μM 50 0.136 ± 0.0154 0.075 ± 0.009 μM 1.1 ± 0.024

Adrenergic α_]D hum 0.3 μM 53 0.377 ± 0.049 μM 0.185 ± 0.024 μM 0.984 ± 0.111

Adrenergic α_2A hum 3 μM 51 2.82 ± 0.275 μM 1.06 ± 0.103 μM 0.969 ± 0.012

Adrenergic α_2B hum 1 μM 62 0.451 ± 0.097 μM 0.206 ± 0.0445 μM 0.902 ± 0.055

Adrenergic α₂c hum lO μM 58 5.64 ± 1.01 μM 0.82 ± 0.146 μM 1.1 ± 0.079

Adrenergic <Xi_* rat 0.1 μM 61 0.0693 μM 0.0372 μM 0.964

Adrenergic α_2* rat 10 μM 73 1.41 μM 1.29 μM 0.592

Receptor binding methods, α-adrenergic subtype specific, are provided below.

Receptor binding methods, α-adrenergic nonselective, are provided below.

These results show that 1 l-piperazin-l-yldibenzo[b_j/][l,4]thiazepine has lower affinity than quetiapine to the αl and α2 adrenergic receptors. Example 6 In vitro assay of 5HTu_^ asonism by 11-piperazin-l-yldibenzofb.fj ' [lΛlthiazeyine

CHO membranes (10 μg protein) expressing human 5-HT_IA receptors were incubated in 100 μl of 2OmM HEPES, pH 7.4 assay buffer containing 1OmM MgCl_2, 10OmM NaCl, 0.1%BSA, 20μM GDP, 200 μg WGA-PVT beads (Amersham RPNQOOOl), 200 pM GTPy³⁵S (Perkin Elmer NEG-030H). 1 l-piperazin-l-yldibenzo[b/|[l,4]thiazepme was incubated with the above at 11 different concentrations varying from lOμM to 17OpM in Packard OptiPlates with shaking for 1.5 hrs at room temperature. 5-HT was used as a positive control, with an EC50 15.5 nM in the assay. One μM of 5-HT was used as maximum agonist activity (100%) for the compound efficacy determination. The plates were centrifuged to settle the beads and measured in a Parkard TopCount. Using this as^"say, 11-piperazin-l- yldibenzo[&/j[l,4]thiazepine is shown to be a partial agonist of 5HT_1A receptor with an EC50 of 310 nM and a maximum efficacy of 66% relative to lμM of 5-HT.

Example 7

Oral Bioavailability l l-piperazm-l-yldibenzo[6/][l,4]thiazepme was administered to 3 Sprague-Dawley rats each either intravenously or orally at doses of 10 umol/kg or 30 umol/kg, respectively, in a sodium citrate (pH 3) formulation. Blood samples were removed from each animal at several timepoints after dosing. The blood samples were centrifuged to produce plasma. Aliquots of each plasma sample were analyzed by an HPLC method with mass spectrometric detection to measure 1 l-piperazin-l-yldibenzo[&,/][l,4]thiazepme. The area under the plasma concentration curves (AUC) constructed from the sample measurements following iv or po administration were used to calculate oral biovailability. The calculated oral bioavailability based on the results of this study was 11% for rat.

A similar study design (different doses for oral and iv administration) was used to calculate oral bioavailability in Beagle dogs (42%) and cynomolgus monkeys (37%). Hence, l l-piperazin-l-yldibenzo[Z?₃/][l,4]thiazepine has shown to be orally bioavailable in three species.

Brain exposure was measured in rats. For concentrations of 11-piperazin-l- yldibenzo[ό/j[l,4]thiazepine in brain, rats were dosed either po or iv (n=3 per dose route). At one hour after compound administration, blood and brain samples were obtained and then processed for analysis using HPLC/MS to measure concentrations of 11-piperazin-l- yldibenzo[5,/][l,4]thiazepine. Average concentrations in rats one hour after oral dosing at 30 umol/kg po was 658 nmol/ml plasma and 2240 nmol/g brain tissue, giving a brain/plasma exposure ratio of 3.4. A similar analysis after iv dosing measured braur.plasma concentration ratios of 4.6 demonstrating penetration of the compound into the CNS.

Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.

Example 8

Hl RECEPTOR BINDING DATA

The Hl receptor binding method was carried out in accordance with the method set out in De Backer MD et al. Biochem. Biophys. Res Commun. 197(3); 1601, 1993. The compound/metabolite binds to the Hl receptor with a K; of 1.15 nM which suggests that it will have an effect on sleep.

Claims

What is claimed is:

1. A method of treating at least one symptom or condition associated with a Substance- Related Disorder in a human comprising administering to the human an oral pharmaceutical composition comprising 1 l-piperazin-l-yldibenzo[&/][l,4]thiazepine or its pharmaceutically acceptable salt in an amount effective to treat the symptom or condition associated with the Disorder.

2. The method as recited in Claim 1 where the Disorder is selected from the group consisting of Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol-Related Disorders, Amphetamine (or Amphetamine-Like)-Related Disorders, Caffeine-Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen-Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related Disorders.

3. A method of treating at least one symptom or condition associated with: 1) Attention- Deficit and Disruptive Behavior Disorders; 2) Eating Disorders; 3) Personality Disorders including Obsessive-Compulsive Personality Disorder or 4) Impulse-Control Disorders in a human comprising administering to the human an amount of 11-piperazin-l- yldibenzo[6/J[l,4]thiazepine or its pharmaceutically acceptable salt effective to treat the condition or symptom associated with the disorder.

4. The method as recited in Claims 1, 2 or 3 wherein the composition is a solid dosage form.

5. The method as recited in Claims 1, 2, 3 or 4 wherein 11-piperazin-l- yldibenzo[έ/|[l,4]thiazepine comprises a free base.

6. The method as recited in Claims 1, 2, 3, 4 or 5 wherein the amount comprises up to about 750 mg per day.

7. The method as recited in Claim 6 wherein the amount comprises from about 75 mg to about 750 mg per day.

8. The method as recited in Claim 6 wherein the amount comprises from about 1 mg to about 600 mg per day.

9. The method as recited in Claim 6 wherein the amount comprises from about 100 mg to about 400 mg per day.

10. An oral pharmaceutical composition comprising the compound of Formula I:

I or a pharmaceutically acceptable salt thereof together with at least one pharmaceutically acceptable carrier or diluent, wherein said compound of Formula I is present in said oral composition in an effective amount for treating at least one symptom or condition associated with a Substance-Related Disorder .

11. The composition as recited in Claim 10 wherein the Disorder is selected from the group consisting of Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol-Related Disorders, Amphetamine (or Amphetamine-Like)- Related Disorders, Caffeine-Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen-Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related.

12. An oral pharmaceutical composition comprising the compound of Formula I:

I or a pharmaceutically acceptable salt thereof together with at least one pharmaceutically acceptable carrier or diluent, wherein said compound of Formula I is present in said oral composition in an effective amount for treating at least one symptom or condition associated with a disorder selected from: 1) Attention-Deficit and Disruptive Behavior Disorders; 2) Eating Disorders; 3) Personality Disorders including but not limited to Obsessive-Compulsive Personality Disorder;or 4) Impulse-Control Disorders Substance-Related Disorder.

13. The composition as recited in Claims 10, 11 or 12 wherein the 11-piperazin-l- yldibenzo[b/][l,4]thiazepine comprises a free base.

14. The composition as recited in Claims 10, 11, 12 or 13 wherein the composition is a solid dosage form.

15. The composition as recited in Claims 10, 11, 12, 13 or 14 wherein the amount is up to about 750 mg.

16. The composition as recited in Claim 15 wherein the amount is from about 75 mg to about 750 mg.

17. The composition as recited in Claim 15 wherein the amount is from about 1 mg to about 600 mg.

18. The composition as recited in Claim 15 wherein the amount is from about 100 mg to about 400 mg.

19. A method of treating at least one symptom or condition associated with a Sleep Disorder in a human comprising administering to the human an oral pharmaceutical composition comprising ll-piperazin-l-yldibenzo[δ/J[l,4]thiazepine or its pharmaceutically acceptable salt in an amount effective to treat the symptom or condition associated with the Disorder.

20. The method as recited in Claim 19 wherein the Disorder is selected from the group consisting of Dyssomnia Disorders and parasomnia Disorders.

21. The method as recited in Claim 20 wherein the Dyssomnia Disorder is selected from the group consisting of Primary insomnia, Primary Hypersomnia and narcolepsy.

22. The¹ method as recited in Claim 20 wherein the Parasomnia Disorder is selected from the group consisting of Nightmare Disorder, Sleep Terror Disorder and Sleepwalking Disorder.

23. The method as recited in Claims 19, 20, 21 or 22 wherein the composition is a solid dosage form.

24. The method as recited in Claims 19, 20, 21, 22, or 23 wherein 11-piperazin-l- yldibenzo[δ_j/_j|[l,4]thiazepine comprises a free base.

25. The method as recited in Claims 19, 20, 21, 22, 23 or 24 wherein the amount comprises up to about 750 mg per day.

26. The method as recited in Claim 25 where the amount comprises from about 75 mg to about 750 mg per day.

27. The method as recited in Claim 25 wherein the amount comprises from about 1 mg to about 600 mg per day.

28. The method as recited in Claim 25 wherein the amount comprises from about 100 mg to about 400 mg per day.

29. An oral pharmaceutical composition comprising the compound of Formula I:

I or a pharmaceutically acceptable salt thereof together with at least one pharmaceutically acceptable carrier or diluent, wherein said compound of Formula I is present in said oral composition in an effective amount for treating at least one symptom or condition associated with a Sleep Disorder .

30. The composition as recited in Claim 29 wherein the Disorder is selected from the group consisting of Dyssomnia Disorders and parasomnia Disorders.

31. The composition as recited in Claim 30 wherein the Dyssomnia Disorder is selected from the group consisting, of Primary insomnia, Primary Hypersomnia and narcolepsy.

32. The composition as recited in Claim 30 wherein the Parasomnia Disorder is selected from the group consisting of Nightmare Disorder, Sleep Terror Disorder or Sleepwalking Disorder.

33. The composition as recited in Claims 29, 20, 31 or 32 wherein the 11-piperazin-l- yldibenzo[ό/j[l,4]thiazepine comprises a free base.

34. The composition as recited in Claims 29, 30, 31, 32 or 33 wherein the composition is a solid dosage form.

35. The composition as recited in Claims 29, 30, 31, 32, 33 or 34 wherein the amount is up to about 750 mg.

36. The composition as recited in Claim 35 wherein the amount is from about 75 mg to about 750 mg.

37. The composition as recited in Claims 35 wherein the amount is from about 1 mg to about 600 mg.

38. The composition as recited in Claims 35 is from about 100 mg to about 400 mg.

39. A method of treating at least one symptom or condition associated with a Disorder Usually First Diagnosed in Infancy, Childhood, or Adolescence in a human comprising . administering to the human an oral pharmaceutical composition comprising 11-piperazin-l- yldibenzo[Z>/][l,4]thiazepine or its pharmaceutically acceptable salt in an amount effective to treat the symptom or condition associated with the Disorder.

40. The method as recited in Claim 39 wherein the composition is a solid dosage form.

41. The method as recited in Claims 39 or 40 wherein 11-piperazin-l- yldibenzo[Z?_j/][l,4]thiazepine comprises a free base.

42. The method as recited in Claims 39, 40 or 41wherein the amount comprises up to about 750 mg.

43. The method as recited in Claim 42 wherein the amount comprises from about 75 mg to about 750 mg.

44. The method as recited in Claim 43 wherein the amount comprises from about 1 mg to about 600 mg.

45. The method as recited in Claim 44 wherein the amount comprises from about 100 mg to about 400 mg.

46. An oral pharmaceutical composition comprising the compound of Formula I:

I or a pharmaceutically acceptable salt thereof together with at least one pharmaceutically acceptable carrier or diluent, wherein said compound of Formula I is present in said oral composition in an effective amount for treating at least one symptom or condition associated with a Disorder Usually First Diagnosed in Infancy, Childhood, or Adolescence.

47. The composition as recited in Claim 46 wherein the 11-piperazin-l- yldibenzo[έ_j/][l,4]thiazepme comprises a free base.

48. The composition as recited in Claims 46 or 47 wherein the composition is a solid dosage form.

49. The composition as recited in Claims 46, 47 or 48 wherein the amount is up to about 750 mg.

50. The composition as recited in claims 49 wherein the amount is from about 75 mg to about 750 mg.

51. The composition as recited in Claim 49 wherein the amount is from about 1 mg to about 600 mg.

52. The composition as recited in Claims 49 wherein the amount is from about 100 mg to about 400 mg.

53. Use of 1 l-piperazin-l-yldibenzo[b_j/][l,4]thiazepine or its pharmaceutically acceptable salt in the treatment of a Substance-Related Disorder in a human comprising administering to the human an amount 1 l-piperazm-l-yldibenzo[δ,/][l,4]thiazepme or its pharmaceutically acceptable salt effective to treat the symptom or condition associated with the disorder.

54. The use recited in Claim 53 where the Disorder is selected from the group consisting of Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol-Related Disorders, Amphetamine (or Amphetamine-Like)-Related Disorders, Caffeine-Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen-Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related Disorders.

55. The use of 1 l-piperazin-l-yldibenzo[6/][l,4]thiazepine or its pharmaceutically acceptable salt in the treatment of at least one symptom or condition associated with: 1) Attention-Deficit and Disruptive Behavior Disorders; 2) Eating Disorders; 3) Personality Disorders including Obsessive-Compulsive Personality Disorder or 4) Impulse-Control Disorders in a human comprising administering to the human an amount of 11-piperazin-l- yldibenzo[&/J[l,4]thiazepine or its pharmaceutically acceptable salt effective to treat the condition or symptom associated with the disorder.

56. The use of 1 l-piperazin-l-yldibenzo[Z?/I[l,4]thiazepine or its pharmaceutically acceptable salt in the treatment of at least one symptom or condition associated with a Sleep Disorder in a human comprising administering to the human an amount of 11-piperazin-l- yldibenzo[έ/J[l,4]thiazepine or its pharmaceutically acceptable salt effective to treat the symptom or condition associated with the Disorder.

57. The use as recited in Claim 56 wherein the Disorder is selected from the group consisting of Dyssomnia Disorders and parasomnia Disorders.

58. The use as recited in Claim 57 wherein the Dyssomnia Disorder is selected from the group consisting of Primary insomnia, Primary Hypersomnia and narcolepsy.

59. The method as recited in Claim 57 wherein the Parasomnia Disorder is selected from the group consisting of Nightmare Disorder, Sleep Terror Disorder and Sleepwalking Disorder.

60. Use of 1 l-piperazin-l-yldibenzo[ό_j/j[l,4]thiazepine or its pharmaceutically acceptable salt in the treatment of at least one symptom or condition associated with a Disorder Usually First Diagnosed in Infancy, Childhood, or Adolescence in a human comprising administering to the human ll-piperazin-l-yldibenzo[&,/][l,4]thiazepine or its pharmaceutically acceptable salt in an amount effective to treat the symptom or condition associated with the Disorder.

61. Use of 11 -piperazin- 1 -yldibenzo [bj] [ 1 ,4]thiazepine or its pharmaceutically acceptable salt in the manufacture of a medicament for the treatment of a Substance-Related Disorder in a human.

62. The use recited in Claim 61 wherein the Disorder is selected from the group consisting of Substance Dependence, Substance Abuse, Substance Intoxication, Substance Withdrawal, Alcohol-Related Disorders, Amphetamine (or Amphetamine-Like)-Related Disorders, Caffeine-Related Disorders, Cannabis-Related Disorders, Cocaine-Related Disorders, Hallucinogen-Related Disorders, Inhalant-Related Disorders, Nicotine-Related Disorders, Opioid-Related Disorders, Phencyclidine (or Phencyclidine-Like)-Related Disorders, and Sedative-, Hypnotic- or Anxiolytic-Related Disorders.

63. The use of 11 -piperazin- l-yldibenzo[ό/|[l,4]thiazepine or its pharmaceutically acceptable salt in the manufacture of a medicament for the treatment of at least one symptom or condition associated with: 1) Attention-Deficit and Disruptive Behavior Disorders; 2) Eating Disorders; 3) Personality Disorders including Obsessive-Compulsive Personality Disorder or 4) Impulse-Control Disorders in a human.

64. The use of 1 l-piperazin-l-yldibenzo[ό/|[l,4]thiazepme or its pharmaceutically acceptable salt in the manufacture of a medicament for the treatment of at least one symptom or condition associated with a Sleep Disorder in a human.

65. The use as recited in Claim 64 wherein the Disorder is selected from the group consisting of Dyssomnia Disorders and parasomnia Disorders.

66. The use as recited in Claim 65 wherein the Dyssomnia Disorder is selected from the group consisting of Primary insomnia, Primary Hypersomnia and narcolepsy.

67. The method as recited in Claim 65 wherein the Parasomnia Disorder is selected from the group consisting of Nightmare Disorder, Sleep Terror Disorder and Sleepwalking Disorder.

68. The use as recited in any one of claims 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 30/31, 32, 33, 34, 35, 36, 37, 38, 46, 47, 48, 49, 50, 51, 52 in the manufacture of a.medicament in the treatment of at least one symptom or condition associated with the Disorder.