WO2006072593A3 - Device for magnet assisted transfer of chemical compounds into cells and method for magnet assisted transfer of proteins into cells - Google Patents

Device for magnet assisted transfer of chemical compounds into cells and method for magnet assisted transfer of proteins into cells Download PDF

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Publication number
WO2006072593A3
WO2006072593A3 PCT/EP2006/000107 EP2006000107W WO2006072593A3 WO 2006072593 A3 WO2006072593 A3 WO 2006072593A3 EP 2006000107 W EP2006000107 W EP 2006000107W WO 2006072593 A3 WO2006072593 A3 WO 2006072593A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
assisted transfer
magnet assisted
protein
disclosed
Prior art date
Application number
PCT/EP2006/000107
Other languages
French (fr)
Other versions
WO2006072593A2 (en
Inventor
Thomas Schmidt
Lothar Germeroth
Original Assignee
Iba Gmbh
Thomas Schmidt
Lothar Germeroth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iba Gmbh, Thomas Schmidt, Lothar Germeroth filed Critical Iba Gmbh
Publication of WO2006072593A2 publication Critical patent/WO2006072593A2/en
Publication of WO2006072593A3 publication Critical patent/WO2006072593A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
    • A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means

Abstract

Disclosed is method for transferring a protein into a cell comprising contacting a protein to be transferred with at least one magnetic particle to form a complex comprising the protein and the magnetic particle, and contacting said complex with a cell in the presence of a suitable permanent magnetic field, thereby transferring the protein into the cell. Also disclosed is such a complex as well as to methods for making it. Further disclosed is a device for magnet assisted transfer or proteins, nucleic acids and other substances, wherein the device comprises a plurality of permanent magnets arranged adjacent to each other in a substantially gap-free and continuous arrangement that creates a substantially homogeneous magnetic surface. Furthermore, disclosed are methods, compositions and kits useful for research, diagnostics and/or therapy.
PCT/EP2006/000107 2005-01-07 2006-01-09 Device for magnet assisted transfer of chemical compounds into cells and method for magnet assisted transfer of proteins into cells WO2006072593A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64231205P 2005-01-07 2005-01-07
US60/642,312 2005-01-07

Publications (2)

Publication Number Publication Date
WO2006072593A2 WO2006072593A2 (en) 2006-07-13
WO2006072593A3 true WO2006072593A3 (en) 2006-12-21

Family

ID=36647841

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/000107 WO2006072593A2 (en) 2005-01-07 2006-01-09 Device for magnet assisted transfer of chemical compounds into cells and method for magnet assisted transfer of proteins into cells

Country Status (1)

Country Link
WO (1) WO2006072593A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0717582D0 (en) * 2007-09-10 2007-10-17 Univ Keele Magnetic transfection device
US9663780B2 (en) 2014-10-15 2017-05-30 Alpaqua Engineering, LLC Solid-core ring-magnet
US11242519B2 (en) 2018-08-23 2022-02-08 Alpaqua Engineering, LLC Discontinuous wall hollow core magnet

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2958019A (en) * 1956-09-17 1960-10-25 Indiana General Corp Magnetic pad assembly
DE1564315A1 (en) * 1966-03-25 1969-09-25 Magnetfab Bonn Gmbh Magnetic system composed of several permanent magnets and the process for their magnetization
DE2127813A1 (en) * 1971-06-04 1972-12-14 Herzog H Magnetized blackboard - of permanently magnetized objects bonded with plastic fill to nonmagnetic baseboard
US4549532A (en) * 1983-07-14 1985-10-29 Horst Baermann Flexible magnetic sheet for therapeutic use
US5304111A (en) * 1992-06-19 1994-04-19 Nikken, Inc. Therapeutic magnetic sheet with repeated curved magnetic areas
US5538495A (en) * 1992-01-21 1996-07-23 Nu-Magnetics Inc. Flexible magnetic pad with multi-directional constantly alternating polarity zones
US5965282A (en) * 1995-09-25 1999-10-12 Rheinmagnet Horst Baermann Gmbh Magnetic arrangement for therapeutic application
EP1080652A1 (en) * 1999-09-01 2001-03-07 Schering-Plough Healthcare Products, Inc. Magnetic sheets
WO2002000870A2 (en) * 2000-06-26 2002-01-03 Christian Plank Method for transfecting cells using a magnetic field
US6416458B1 (en) * 2000-07-12 2002-07-09 Therion Research Inc. Therapeutic flexible magnetic sheet and method
WO2003002260A1 (en) * 2001-06-27 2003-01-09 bioMérieux Method, device and equipment for the wet separation of magnetic microparticles
EP1378920A1 (en) * 2001-03-23 2004-01-07 Sumitomo Special Metals Company Limited Magnetic field generator
WO2004006765A1 (en) * 2002-07-17 2004-01-22 Dailey James P Delivery of therapeutic agent affixed to magnetic particle
WO2004024910A1 (en) * 2002-09-12 2004-03-25 Genovis Ab Particle for magnetically induced membrane transport

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2958019A (en) * 1956-09-17 1960-10-25 Indiana General Corp Magnetic pad assembly
DE1564315A1 (en) * 1966-03-25 1969-09-25 Magnetfab Bonn Gmbh Magnetic system composed of several permanent magnets and the process for their magnetization
DE2127813A1 (en) * 1971-06-04 1972-12-14 Herzog H Magnetized blackboard - of permanently magnetized objects bonded with plastic fill to nonmagnetic baseboard
US4549532A (en) * 1983-07-14 1985-10-29 Horst Baermann Flexible magnetic sheet for therapeutic use
US4549532B1 (en) * 1983-07-14 1998-08-11 Horst Baermann Flexible magnetic sheet for therapeutic use
US5538495A (en) * 1992-01-21 1996-07-23 Nu-Magnetics Inc. Flexible magnetic pad with multi-directional constantly alternating polarity zones
US5304111A (en) * 1992-06-19 1994-04-19 Nikken, Inc. Therapeutic magnetic sheet with repeated curved magnetic areas
US5965282C1 (en) * 1995-09-25 2002-05-07 Baermann Horst Rheinmagnet Magnetic arrangement for therapeutic application
US5965282A (en) * 1995-09-25 1999-10-12 Rheinmagnet Horst Baermann Gmbh Magnetic arrangement for therapeutic application
EP1080652A1 (en) * 1999-09-01 2001-03-07 Schering-Plough Healthcare Products, Inc. Magnetic sheets
WO2002000870A2 (en) * 2000-06-26 2002-01-03 Christian Plank Method for transfecting cells using a magnetic field
US6416458B1 (en) * 2000-07-12 2002-07-09 Therion Research Inc. Therapeutic flexible magnetic sheet and method
EP1378920A1 (en) * 2001-03-23 2004-01-07 Sumitomo Special Metals Company Limited Magnetic field generator
WO2003002260A1 (en) * 2001-06-27 2003-01-09 bioMérieux Method, device and equipment for the wet separation of magnetic microparticles
WO2004006765A1 (en) * 2002-07-17 2004-01-22 Dailey James P Delivery of therapeutic agent affixed to magnetic particle
WO2004024910A1 (en) * 2002-09-12 2004-03-25 Genovis Ab Particle for magnetically induced membrane transport

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FRENZEL A ET AL: "Novel purification system for 6xHis-tagged proteins by magnetic affinity separation", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 793, no. 2, 15 August 2003 (2003-08-15), pages 325 - 329, XP004442700, ISSN: 1570-0232 *

Also Published As

Publication number Publication date
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