WO2006071988A1 - Thienopyrimidine derivatives as phosphodiesterase 10 inhibitors - Google Patents

Thienopyrimidine derivatives as phosphodiesterase 10 inhibitors Download PDF

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WO2006071988A1
WO2006071988A1 PCT/US2005/047439 US2005047439W WO2006071988A1 WO 2006071988 A1 WO2006071988 A1 WO 2006071988A1 US 2005047439 W US2005047439 W US 2005047439W WO 2006071988 A1 WO2006071988 A1 WO 2006071988A1
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alkyl
chosen
substituted
alkoxy
phenyl
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PCT/US2005/047439
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French (fr)
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Ruiping Liu
Mark Phillip Arrington
Allen Hopper
Ashok Tehim
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Memory Pharmaceuticals Corp.
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Priority to EP05855928A priority Critical patent/EP1831229A1/en
Priority to CA002594676A priority patent/CA2594676A1/en
Priority to JP2007548601A priority patent/JP2008525500A/en
Priority to AU2005321903A priority patent/AU2005321903A1/en
Priority to MX2007007387A priority patent/MX2007007387A/en
Publication of WO2006071988A1 publication Critical patent/WO2006071988A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/08—Antiepileptics; Anticonvulsants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/22—Anxiolytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/12—Antidiuretics, e.g. drugs for diabetes insipidus

Definitions

  • cAMP and cGMP cyclic nucleotide monophosphates
  • PKA cAMP-dependent protein kinase
  • Downstream mediators of cGMP signaling also include kinases and ion channels. In addition to actions mediated by kinases, cAMP and cGMP bind directly to some cellular proteins and directly regulate their activity.
  • Cyclic nucleotides are produced from the actions of adenylyl cyclase and guanylyl cyclase which convert ATP to cAMP and GTP to cGMP, respectively. Extracellular signals, often through the actions of G protein-coupled receptors, regulate the activity of the cyclases. Alternatively, the amount of cAMP and cGMP may be altered by regulating the activity of the enzymes that degrade cyclic nucleotides. Cell homeostasis is maintained by the rapid degradation of cyclic nucleotides after stimulus- induced increases. The enzymes that degrade cyclic nucleotides are called 3',5'-cyclic nucleotide-specific phosphodiesterases (PDEs).
  • PDEs 3',5'-cyclic nucleotide-specific phosphodiesterases
  • PDE-PDEl 1 Eleven PDE gene families (PDEl-PDEl 1) have been identified so far, based on their distinct amino acid sequences, catalytic and regulatory characteristics, and sensitivity to small molecule inhibitors. These PDE families are coded for by 21 genes; and further multiple splice variants are transcribed from many of these genes. Expression patterns of each of the gene families are distinct. PDEs differ with respect to their affinity for cAMP and cGMP. Activities of different PDEs are regulated by different signals.
  • PDEl is stimulated by Ca 2+ /calmodulin
  • PDE2 activity is stimulated by cGMP
  • PDE3 is inhibited by cGMP
  • PDE4 is cAMP specific and is specifically inhibited by rolipram
  • PDE5 is cGMP-specific
  • PDE6 is expressed in retina. Less is known about the expression patterns and functional attributes of the higher number PDEs (7 through 11).
  • PDEl 0 sequences were first identified by using bioinformatics and sequence information from other PDE gene families (Fujishige et al., J. Biol. Chem. 274:18438-18445, 1999; Loughney, K. et al., Gene 234:109-117, 1999; Soderling, S. et al., Proc. Natl. Acad. Sci. USA 96:7071-7076, 1999).
  • PDElO is defined as a unique gene family based on its amino acid sequence, functional properties and tissue distribution. The human PDElO gene is large, over 200 kb, with up to 24 exons coding for each of the splice variants.
  • the amino acid sequence is characterized by two GAF domains (which bind cGMP), a catalytic region, and alternatively spliced N and C termini. Numerous splice variants are possible because of at least 3 alternative exons encoding the N and 2 for the C-termini.
  • PDElOAl a splice variant of PDElO, is a 779 amino acid protein that hydrolyzes both cAMP and cGMP.
  • the K 1n values for cAMP and cGMP are 0.05 and 3.0 micromolar, respectively.
  • several variants with high homology have been isolated from both rat and mouse tissues and sequence banks.
  • PDEl 0 transcripts were initially detected in RNA from human testis and brain. Immunohistochemical analysis identified specific brain regions enriched in PDElO. The basal ganglia express the highest amounts of PDElO. Specifically, striatal neurons in the olfactory tubercle, caudate nucleus and nucleus accumbens are enriched in PDElO (Seeger, T. F. et al., Brain Res. 2003, Sept. 26, 985(2): 113-26). Western blots did not reveal the expression of PDElO in other brain tissues, although immunprecipitation of the PDElO complex was possible in hippocampal and cortical tissues.
  • PDElO inhibitors may play an important role in the basal ganglia.
  • PDElO selective inhibitors could be used to raise levels of cAMP and/or cGMP within cells that express the PDElO enzyme, especially neurons that comprise the basal ganglia.
  • Selective PDElO inhibition could lead to altered basal ganglia function and may be effective in treating a variety of neuropsychiatric conditions involving the basal ganglia.
  • heteroaryl and (vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C]-S alkyl, halogenated Q-g alkyl, Ci- 4 alkoxy, halogenated C 1 -4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci- 8 alkylamino, di-Ci- 8 alkylamino, Cp 8 alkylsulphinyl, C 1 - S alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • R 2 is chosen from C 2 - 4 hydroxyalkyl, C 2 - 4 alkenyl, C 2 - 4 alkenylene-R 5 , C 2 - 4 alkynyl, C 2 - 4 alkynylene-R 5 , -O-arylene-Y-R 5 , -X(CR 3 R 4 ) n YR 5 and - (CR 6 R 7 ) m YR 5 where:
  • X is chosen from -O-, -S- and -NR 9 - where R 9 is chosen from H, Ci- 8 alkyl; and C 3 - 8 cycloalkyl, substituted Cp 8 alkyl; and substituted C 3 - 8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo;
  • Y is chosen from a covalent bond, -O-, -S- and -NR 9 -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
  • R 3 and R 4 are each independently chosen from: 0) H; (ii) halo; (iii) C M2 alkyl; (iv) C 2-12 alkenyl; (v) C 2-I2 alkynyl; (vi) C 3 - 12 cycloalkyl;
  • substituted Cj -I2 alkyl chosen from mono-, di-, and tri-substituted C 1-12 alkyl and wherein the substituents are independently chosen from halo, hydroxy, Ci ⁇ -alkoxy, halogenated C 1-4 alkoxy, nitro, cyano, carboxy, amino, Ci -4 alkylamino, di-Cj ⁇ -alkylamino, Ci ⁇ -hydroxyalkyl, C 2-4 -hydroxyalkoxy, Ci- 4 -alkylthio, Ci ⁇ -alkylsulphinyl, and Ci -4 - alkylsulphonyl;
  • R 5 is chosen from C 6- I 0 aryl, substituted C 6-J0 aryl, Het, substituted Het, C 6- I 0 aryl- Cr 8 alkyl, substituted C 6-I0 aryl-Ci- 8 alkyl, Het-Ci-g alkyl, and substituted Het-Ci- 8 alkyl, wherein the alkyl portions of C 6-I0 aryl-Ci-g alkyl and Het- Ci-g alkyl are optionally substituted by oxo and wherein: substituted C 6-I0 aryl is chosen from mono-, di-, and tri-substituted C 6-10 aryl wherein the substituents are independently chosen from
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci-g alkyl, halogenated Ci-g alkyl, C 1 - 4 alkoxy, halogenated C 1 -4 alkoxy, hydroxy Ci-g alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci-g alkylamino, di-Ci-g alkylamino, Ci-g alkylsulphinyl, Ci- 8 alkylsulphonyl, Q-g alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Cr 8 alkyl, C 1 - 4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Q-s alkylamino, C 1 - 8 alkylsulphinyl, Cr 8 alkylsulphonyl, Ci- 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio; and
  • Het is chosen from heterocyclyl, substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl, heteroaryl, and substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl, wherein the substituents are independently chosen from
  • substituted C 6 - 20 aryl chosen from mono-, di-, and tri-substituted C 6 - 20 aryl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Cr 8 alkyl, Cr 4 alkoxy, halogenated Cr 4 alkoxy, Cr 8 hydroxyalkyl, C 2 - 8 hydroxyalkoxy, amino, Crs alkylamino, di-Crs alkylamino, Ci - 8 alkylsulphinyl, Cj - 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, and thio,
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Crs alkyl, Cr 4 alkoxy, halogenated Cj -4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Cr 8 alkylamino, Crs alkylsulphinyl, Cr 8 alkylsulphonyl, Crs alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Cr 8 alkyl, Cr 4 alkoxy, halogenated Ci-4 alkoxy, hydroxy C ⁇ s alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Crs alkylamino, Cp 8 alkylsulphinyl, C 1 - S alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cr 8 alkyl, halogenated C 1 - S alkyl, Cr 4 alkoxy, halogenated Cr 4 alkoxy, hydroxy C 1 - S alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Ci- 8 alkylamino, C j-g alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cr 8 alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio, and
  • R 9 is chosen from H, Cp 8 alkyl, C 3 - 8 cycloalkyl, substituted Cp 8 alkyl chosen from mono-, di-, and trisubstituted Cp 8 alkyl, and substituted C 3 - 8 cycloalkyl chosen from mono-, di-, and trisubstituted C 3 - 8 cycloalkyl wherein the substituents are independently chosen from halo, Cp 4 -alkyl, Cp 4 -alkoxy, and oxo; and provided that the compound of Formula I is not chosen from :
  • R 2 is not -O-p-phenylene-O- (CH 2 )phenyl, -O-p-phenylene-NH-C(O)-thiophen-2-yl, -O-(CH 2 ) 2 -O-(phenyl), - NH(CH 2 ) 2 -(4-hydroxyphenyl), -O-(CH 2 ) 2 -(l,3-dioxoisoindolin-l-yl), -NH(CH 2 ) 2 -(2-oxo- imidaziolidin-1-yl), -NH(CH 2 ) 2 -(3,4,-dimethoxyphenyl), -S(CH 2 ) 2 -phenyl, -NH(CH 2 ) 2 - phenyl, -NH(CH 2 ) 2 -morpholin-4-yl, -NH(CH 2 ) 2 -thiophen-2-yl,
  • R 2 is not -O-p-phenylene-NH-C(O)-3,-4- dimethoxyphenyl, -O-(CH 2 ) 2 -O-(4-methylphenyl), -O-(CH 2 ) 2 -Ophenyl, -O-(CH 2 ) 2 -O(3- bromophenyl), -NH-(CH 2 ) 2 -NH(3-CF 3 -pyridin-2-yl), -S-(CH 2 ) 2 -O-(3-methylphenyl), -S- (CH 2 ) 2 -O-(4-Fphenyl), -S-(CH 2 ) 2 -O-(2,4-dichlorophenyl), -NH-(CH 2 ) 2 -NH(4-CF 3 - ⁇ yrimidin-2-yl), -NH-(CH 2 ) 2 -O-(3-ace
  • R 2 is not chosen from -O-(CH 2 ) 2 -O-(2- chlorophenyl), -O-(CH 2 ) 2 -O-(4-fluorophenyl), -S-(CH 2 ) 3 -phenyl, -NH-(CH 2 ) 3 -phenyl, - NH-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 3 -mo ⁇ holin-4-yl, -NH-(CH 2 ) 3 -(2-oxo-pyrrolidin-l-yl), - NH-(CH 2 ) 2 -morpholin-4-yl, -S-(CH 2 ) 2 - ⁇ henyl, -NH-(CH 2 ) 2 -azepan-l-yl, -S-(CH 2 ) 2 - mo ⁇ holin-4-yl, -NH-(CH 2 )
  • R 1 is 4-chlorophenyl
  • R 2 is not chosen from -O-p-phenylene-O- CH 2 -phenyl, -O-(CH 2 ) 2 -O-(4-methylphenyl), -S-(CH 2 ) 4 -(l,3-dioxoisoindolin-l-yl), -S- (CH 2 ) 3 -phenyl, -S-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 3 -imidazol-l-yl, -NH-(CH 2 ) 3 -morpholin-4- yl, -NH-(CH 2 ) 2 -morpholin-4-yl, -NH-(CH 2 ) 2 -azepan-l-yl, -S-(CH 2 ) 2 -(1,3- dioxoisoindolin-1-yl), -NH-(CH 2 ) 2 -(3
  • R 2 when R 1 is 3-thiophen-2-yl, then R 2 is not chosen from -NH-(CH 2 ) 2 - morpholin-4-yl, -NH-(CH 2 ) 3 -morpholin-4-yl, -NH-(CH 2 ) 2 -phenyl, and -NH-(CH 2 ) 2 -(3 ,4- dimethoxyphenyl); (ix) when R 1 is 4-methoxyphenyl, then R 2 is not chosen from -S-(CH 2 ) 3 - phenyl, -NH-(CH 2 ) 2 -morpholin-4-yl, -NH-(CH 2 ) 2 -C(CH 3 ) 2 -morpholin-4-yl, - NHCH 2 C(CH 3 ) 2 -mo ⁇ holin-4-yl, -N(CH 3 )-(CH 2 ) 2 -(3 ,4-dimethoxyphenyl), -S-(CH 2
  • R 2 is not chosen from -O-p- phenylene-O-CH 2 -phenyl, -S-(CH 2 ) 3 -phenyl, -S-(CH 2 ) 3 -morpholin-4-yl, -S-(CH 2 ) 2 - phenyl, -NH-(CH 2 ) 3 -(4-hydroxyphenyl), -0-(CH 2 ) 2 -(l,3-dioxoisoindolin-l-yl), -NH- (CH 2 ) 2 - mo ⁇ holin-4-yl, -NH-(CH 2 ) 3 - morpholin-4-yl, -NH-(CH 2 ) 2 -(3,4- methylenedioxyphenyl), -S-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 2 -phenyl, -NH-(CH 2 )
  • R 2 is not chosen from -(CH 2 ) 3 -phenyl, -S- (CH 2 ) 3 -mo ⁇ holin-4-yl, -S-(CH 2 ) 2 -phenyl, -S-(CH 2 ) 3 - ⁇ henyl, -NH-(CH 2 ) 3 -mo ⁇ holin-4- yl, -NH-(CH 2 ) 3 -(2-oxo-pyrrolidin-l-yl), and -NH-(CH 2 ) 2 -(3,4-ethylenedioxyphenyl); and
  • R 2 is not -NH-(CH 2 ) 2 -(3,4- ethylenedioxyphenyl);
  • R 1 when R 1 is 2,3-dihydro-l ,4-benzodioxin-2-yl, then R 2 is not NH(CH 2 ) 2 - phenyl, and
  • substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, C 1 - S alkyl, halogenated Ci- 8 alkyl, Ci- 4 alkoxy, halogenated Q- 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci-g alkylamino, di-Ci-g alkylamino, Ci- 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cj- 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C i- 8 alkyl, halogenated Ci - 8 alkyl, Q- 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci- 8 alkylamino, di-Ci- 8 alkylamino, Ci- 8 alkylsulphinyl, Q-s alkylsulphonyl, Q-s alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • R 2 is chosen from C 2 - 4 hydroxyalkyl, C 2 - 4 alkenyl, C 2 - 4 alkenylene-R 5 , C 2 - 4 alkynyl, C 2 - 4 alkynylene-R 5 , -O-arylene-Y-R 5 , -X(CR 3 R 4 ) ⁇ YR 5 and - (CR 6 R 7 ) m YR 5 where:
  • X is chosen from -0-, -S- and -NR 9 -;
  • Y is chosen from a covalent bond, -0-, -S- and -NR 9 -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
  • R 3 and R 4 are each independently chosen from: (i) H; (ii) halo; (iii) C M2 alkyl; (iv) C 2-I2 alkenyl; (v) C 2-I2 alkynyl; (vi) C 3 - I2 cycloalkyl;
  • R 6 and R 7 are independently chosen from 0) H; (ii) halo; (iii) C 1-12 alkyl; (iv) C 2-I2 alkenyl; (v) C 2-I2 alkynyl; (vi) C 3 - I2 cycloalkyl;
  • R 9 is chosen from H, Ci-g alkyl, C 3 -g cycloalkyl, substituted Ci- 8 alkyl chosen from mono-, di-, and trisubstituted Q-g alkyl, and substituted C 3 - « cycloalkyl chosen from mono-, di-, and trisubstituted C 3 - 8 cycloalkyl wherein the substituents are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo; and
  • R 5 is chosen from pyridin-3-yl and pyridin-4-yl, each of which is optionally substituted with one or two groups chosen from
  • substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Ci- 8 alkyl, Ci- 4 alkoxy, halogenated Q- 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci- 8 alkylamino, di-Cr 8 alkylamino, Cp 8 alkylsulphinyl, Ci - 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, C 1 - S alkyl, halogenated Cj- 8 alkyl, Cr 4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Cpg alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Crs alkylamino, C 1 -S alkylsulphinyl, Cr 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated C 1 - S alkyl, Cr 4 alkoxy, halogenated Cr 4 alkoxy, hydroxy Crs alkyl, hydroxy C 2 - 8 alkoxy, amino, Crs alkylamino, di-Ci- 8 alkylamino, C 1 -S alkylsulphinyl, C 1 -S alkylsulphonyl, C,- 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci- 8 alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cps alkylamino, C 1 - S alkylsulphinyl, d- 8 alkylsulphonyl, Ci- 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, Ci-s alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio; R is chosen from C 2 - 4 hydroxyalkyl, C 2 - 4 alkenyl, C 2 - 4 alkenylene-R 5 , C 2 - 4 alkynyl, C 2 - 4 alky
  • X is chosen from -O-, -S- and -NR 9 - where R 9 is chosen from H, C 1 - S alkyl; and C 3 - 8 cycloalkyl, substituted Cj-g alkyl; and substituted C 3 - 8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo;
  • Y is chosen from a covalent bond, -O-, -S- and -NR 9 -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
  • R and R 4 are each independently chosen from: (i) H; (ii) halo; (iii) C 1-12 alkyl; (iv) C 2-I2 alkenyl; (v) C 2-J2 alkynyl; (vi) C 3 - I2 cycloalkyl;
  • R and R are independently chosen from (i) H; (ii) halo; (iii) Ci -12 alkyl; (iv) C 2-I2 alkenyl; (v) C 2- I 2 alkynyl; (vi) C 3 - J2 cycloalkyl;
  • R 9 is chosen from H, Cr 8 alkyl, C 3 - 8 cycloalkyl, substituted Cp 8 alkyl chosen from mono-, di-, and trisubstituted C)-g alkyl, and substituted C 3 - 8 cycloalkyl chosen from mono-, di-, and trisubstituted C 3 - 8 cycloalkyl wherein the substituents are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo; and
  • R 5 is chosen from pyridin-2-yl and pyridin-4-yl, each of which is substituted with one or two groups chosen from
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C 1 - S alkyl, halogenated Cp 8 alkyl, C 1 -4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Ci- 8 alkylamino, Cr 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, C ⁇ 8 alkyl, halogenated C 1 - 8 alkyl, Cp 4 alkoxy, halogenated C 1 - 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Ci- 8 alkylsulphinyl, Ci- 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Ci- 8 alkyl, halogenated C 1 - S alkyl, C1- 4 alkoxy, halogenated C 1 -4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Ci- 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • heteroaryl-carbonyl and (54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Ci- 8 alkyl, halogenated Cr 8 alkyl, C]- 4 alkoxy, halogenated C 1 - 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Ci- 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, C r8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • substituents are independently chosen from halo, Ci- 8 alkyl, halogenated Cr 8 alkyl, C]- 4 alkoxy, halogen
  • substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C 1 - 8 alkyl, halogenated Cj- 8 alkyl, C 1 - 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci-g alkylamino, di-Ci- 8 alkylamino, Cp 8 alkylsulphinyl, Ci- 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • R 2 is chosen from C 2 - 4 hydroxyalkyl, C 2 - 4 alkenyl, C 2 - 4 alkenylene-R 5 , C 2 - 4 alkynyl, C 2 - 4 alkynylene-R 5 , -O-arylene-Y-R 5 , -X(CR 3 R 4 ) n YR 5 and - (CR 6 R 7 ) m YR 5 where:
  • X is chosen from -O-, -S- and -NR 9 - where R 9 is chosen from H, Ci- 8 alkyl; and C 3 - 8 cycloalkyl, substituted Cp 8 alkyl; and substituted C 3 - 8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo;
  • Y is chosen from a covalent bond, -O-, -S- and -NR -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
  • R 3 and R 4 are each independently chosen from: (i) H; (ii) halo; (iii) C M2 alkyl; (iv) C 2-I2 alkenyl; (v) C 2-I2 alkynyl; (vi) C 3 - I2 cycloalkyl;
  • R 6 and R 7 are independently chosen from (i) H; (ii) halo; (iii) Ci 2 alkyl; (iv) C 2-I2 alkenyl; (v) C 2-J2 alkynyl; (vi) C 3 - I2 cycloalkyl;
  • Ci -I2 alkyl chosen from mono-, di-, and tri-substituted Ci -I2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci -4 -alkoxy, halogenated C 1-4 alkoxy, nitro, cyano, carboxy, amino, Ci -4 alkylamino, di-Ci ⁇ -alkylamino, Ci- 4 -hydroxyalkyl, C 2-4 - hydroxyalkoxy, Ci -4 -alkylthio, Ci -4 -alkylsulphinyl, and Ci -4 - alkylsulphonyl;
  • R 9 is chosen from H, Ci- 8 alkyl, C 3 - 8 cycloalkyl, substituted Cp 8 alkyl chosen from mono-, di-, and trisubstituted Cj- 8 alkyl, and substituted C 3 - 8 cycloalkyl chosen from mono-, di-, and trisubstituted C 3 - 8 cycloalkyl wherein the substituents are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo; p is chosen from 1, 2, 3, and 4; and for each occurrence, R 10 is independently chosen from
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cp 8 alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • composition comprising at least one chemical entity described herein and a pharmaceutically acceptable carrier.
  • Also provided is a method of selectively inhibiting PDElO enzyme in a patient in need thereof comprising administering to said patient an effective amount of at least one chemical entity described herein.
  • Halo herein refers to F, Cl, Br, and I, such as F and Cl.
  • Alkyl means a straight-chain or branched-chain aliphatic hydrocarbon radical. Suitable alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, and dodecyl.
  • alkyl groups include 1-, 2- or 3-methylbutyl, 1,1-, 1,2- or 2,2-dimethylpropyl, 1-ethylpropyl, 1-, 2-, 3- or 4-methylpentyl, 1,1-, 1,2-, 1,3-, 2,2-, 2,3- or 3,3-dimethylbutyl, 1- or 2-ethylbutyl, ethylmethylpropyl, trimethylpropyl, methylhexyl, dimethylpentyl, ethylpentyl, ethylmethylbutyl, dimethylbutyl, and the like.
  • Alkenyl refers to an unsaturated branched or straight-chain alkyl group having at least one carbon-carbon double bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkene.
  • the group may be in either the cis or trans configuration about the double bond(s).
  • Typical alkenyl groups include, but are not limited to, ethenyl; propenyls such as prop-1-en-l-yl, prop-l-en-2-yl, prop-2-en-l-yl (allyl), prop-2-en-2-yl, cycloprop-1-en-l-yl; cycloprop-2-en-l-yl; butenyls such as but-1-en-l-yl, but-l-en-2-yl, 2-methyl-prop-l-en-l-yl, but-2-en-l-yl, but-2-en-l-yl, but-2-en-2-yl, buta-l,3-dien-l-yl, buta-l,3-dien-2-yl, cyclobut-1-en-l-yl, cyclobut-l-en-3-yl, cyclobuta-l,3-dien-l-yl; and the like.
  • an alkenyl group such as
  • Alkynyl refers to an unsaturated branched or straight-chain alkyl group having at least one carbon-carbon triple bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkyne.
  • Typical alkynyl groups include, but are not limited to, ethynyl; propynyls such as prop-1-yn-l-yl, prop-2-yn-l-yl; butynyls such as but-1-yn-l-yl, but-l-yn-3-yl, but-3-yn-l-yl; and the like.
  • an alkynyl group has from 2 to 20 carbon atoms and in other embodiments, from 3 to 6 carbon atoms.
  • Alkynylene refers to a divalent alkynyl group.
  • Cycloalkyl herein refers to a non-aromatic monocyclic, bicyclic, or tricyclic carbocyclic ring, usually having from 3 to 8 ring carbon atoms, for example 4 to 6 ring carbon atoms. The ring may be saturated or have one or more carbon-carbon double bonds.
  • cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl, as well as bridged and caged saturated ring groups such as norbornane.
  • Suitable cycloalkyl groups include spiropentyl, bicyclo[2.1.0]pentyl, bicyclo[3.1.0]hexyl, spiro[2.4]heptyl, spiro[2.5]octyl, bicyclo[5.1.0]octyl, spiro[2.6]nonyl, bicyclo[2.2.0]hexyl, spiro[3.3]heptyl, and bicyclo[4.2.0]octyl. [021] In the arylalkyl groups and heteroarylalkyl groups, "alkyl" refers to a divalent alkylene group having, in certain embodiments, 1 to 4 carbon atoms.
  • alkyl is a substituent (e.g., alkyl substituents on aryl and heteroaryl groups) or is part of a substituent (e.g., in the alkylamino, dialkylamino, hydroxyalkyl, hydroxyalkoxy, alkylthio, alkylsulphinyl, and alkylsulphonyl substituents)
  • the alkyl portion usually has 1 to 12 carbon atoms, for example 1 to 8 carbon atoms, such as 1 to 4 carbon atoms.
  • Aryl as a group or substituent per se or as part of a group or substituent, refers to an aromatic, monocyclic or bicyclic carbocyclic radical containing 6 to 14 carbon atoms, such as 6 to 12 carbon atoms, for example 6 to 10 carbon atoms.
  • Suitable aryl groups include phenyl, naphthyl and biphenyl.
  • substituted aryl groups include the above-described aryl groups which are substituted by at least one time, for example, one, two, or three times, by a group chosen from halo, alkyl, hydroxy, alkoxy, nitro, methylenedioxy, ethylenedioxy, amino, alkylamino, dialkylamino, hydroxyalkyl, hydroxyalkoxy, carboxy, cyano, acyl, alkoxycarbonyl, alkylthio, alkylsulphinyl, alkylsulphonyl, phenoxy, and acyloxy (e.g., acetoxy).
  • Arylalkyl refers to an aryl-alkyl-radical in which the aryl and alkyl portions are in accordance with the previous descriptions. Suitable examples include 1- phenethyl, 2-phenethyl, phenpropyl, phenbutyl, phenpentyl, and naphthylenemethyl.
  • Heteroaryl encompasses: 5- to 7-membered aromatic, monocyclic rings containing one or more, for example, from 1 to 4, or in certain embodiments, from 1 to 3, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon; bicyclic heterocyclyl rings containing one or more, for example, from 1 to 4, or in certain embodiments, from 1 to 3, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring; and tricyclic heterocyclyl rings containing one or more, for example, from 1 to 5, or in certain embodiments, from 1 to 4, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring.
  • heteroaryl includes a 5- to 7-membered heterocyclyl, aromatic ring fused to a 5- to 7-membered cycloalkyl or heterocyclyl ring.
  • bicyclic heteroaryl ring systems wherein only one of the rings contains one or more heteroatoms, the point of attachment may be at either ring.
  • the total number of S and O atoms in the heteroaryl group exceeds 1, those heteroatoms are not adjacent to one another.
  • the total number of S and O atoms in the heteroaryl group is not more than 2.
  • the total number of S and O atoms in the aromatic heterocycle is not more than 1.
  • heteroaryl groups include, but are not limited to, furyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, pyridyl, pyrimidinyl, indolyl, quinolinyl, naphthyridinyl, azaindolyl (e.g.,7-azaindolyl), 1,2,3,4,- tetrahydroisoquinolyl, and the like.
  • heteroaryl groups include, but are not limited to 2-thienyl, 3 -thienyl, 2-, 3- or 4-pyridyl, 2-, 3-, 4-, 5- , 6-, 7- or 8-quinolinyl, 7- azaindolyl, and 1-, 3-, 4-, 5-, 6-, 7- or 8-isoquinolinyl.
  • substituted heteroaryl groups refer to the heteroaryl groups described above which are substituted in one or more places by halo, aryl, alkyl, alkoxy, cyano, halogenated alkyl (e.g., trifluoromethyl), nitro, oxo, amino, alkylamino, and dialkylamino.
  • Heteroaryl does not encompass or overlap with aryl, cycloalkyl, or heterocyclyl, as defined herein
  • Substituted heteroaryl also includes ring systems substituted with one or more oxide (-0 ' ) substituents, such as pyridinyl N-oxides.
  • Heteroarylalkyl refers to a heteroaryl-alkyl-group wherein the heteroaryl and alkyl portions are in accordance with the previous discussions. Suitable examples are pyridylmethyl, thienylmethyl, pyrimidinylmethyl, pyrazinylmethyl, isoquinolinylmethyl, pyridylethyl and thienylethyl.
  • heterocyclo alkyl or heterocyclyl is meant a single, non-aromatic ring, usually with 3 to 7 ring atoms, containing at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms.
  • Heterocyclyl also includes bicyclic ring systems wherein one non-aromatic ring, usually with 3 to 7 ring atoms, contains at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms; and the other ring, usually with 3 to 7 ring atoms, optionally contains 1-3 heteratoms independently selected from oxygen, sulfur, and nitrogen and is not aromatic.
  • the ring or rings may be saturated or have one or more carbon-carbon double bonds.
  • Suitable heterocyclyl groups include, for example (as numbered from the linkage position assigned priority 1), 3-tetrahydrofuranyl, piperidinyl, imidazolinyl, imidazolidinyl, pyrrolinyl, pyrrolidinyl, morpholinyl, piperazinyl, oxazolidinyl, and indolinyl.
  • oxo 0
  • oxide 0
  • Acyl refers to alkanoyl radicals having 2 to 4 carbon atoms, such as formyl, acetyl, propionyl, and butanoyl.
  • Substituted radicals usually have 1 to 3 substituents, for example, 1 or 2 substituent, such as 1 substituent.
  • Compounds of Formula I include, but are not limited to, optical isomers of compounds of Formula I, racemates, and other mixtures thereof.
  • the single enantiomers or diastereomers i.e., optically active forms
  • Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral high- pressure liquid chromatography (HPLC) column.
  • Substantially pure enantiomers contain no more than 5% w/w of the corresponding opposite enantiomer, such as no more than 2%, for example, no more than 1%.
  • compounds of Formula I include Z- and E- forms (or cis- and trans- forms) of compounds with carbon-carbon double bonds.
  • chemical entities of the present invention include all tautomeric forms of the compound.
  • compositions recited herein include pharmaceutically acceptable salts, solvates, crystal forms (including polymorphs and clathrates), chelates, non-covalent complexes, prodrugs, and mixtures thereof.
  • the compounds described herein are in the form of pharmaceutically acceptable salts.
  • the terms "chemical entity” and “chemical entities” also encompass pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures.
  • a polymorph is a solid crystalline phase of a compound with at least two different arrangements or polymorphic forms of that compound molecule in the solid state.
  • Polymorphic forms of any given compound are defined by the same chemical formula or composition and are as distinct in chemical structure as crystalline structures of two different chemical compounds.
  • Pharmaceutically acceptable salts include those obtained by reacting the main compound, functioning as a base, with an inorganic or organic acid to form a salt, for example, salts of hydrochloric acid, sulfuric acid, phosphoric acid, methanesulfonic acid, camphorsulfonic acid, oxalic acid, maleic acid, succinic acid and citric acid.
  • acid salts that can be obtained by reaction with inorganic or organic acids: acetates, adipates, alginates, citrates, aspartates, benzoates, benzenesulfonates, bisulfates, butyrates, camphorates, digluconates, cyclopentanepropionates, dodecylsulfates, ethanesulfonates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, fumarates, hydrobromides, hydroiodides, 2-hydroxy-ethanesulfonates, lactates, maleates, methanesulfonates, nicotinates, 2-naphthalenesulfonates, oxalates, palmoates, pectinates, persulfates, 3-phenylpropionates, picrates, pivalates, propionates, succinates
  • prodrugs also fall within the scope of chemical entities, for example ester or amide derivatives of the compounds of Formula I.
  • chemical entities for example ester or amide derivatives of the compounds of Formula I.
  • prodrugs includes any compounds that become compounds of Formula I when administered to a patient, e.g., upon metabolic processing of the prodrug.
  • prodrugs include, but are not limited to, acetate, formate, and benzoate and like derivatives of functional groups (such as alcohol or amine groups) in the compounds of
  • solvate refers to the chemical entity formed by the interaction of a solvent and a compound. Solvates may also form when solvent molecules are incorporated into the crystalline lattice structure of the compound molecule during the crystallization process. Suitable solvates are pharmaceutically acceptable solvates, such as hydrates, e.g., monohydrates, dihydrates, sesquihydrates, and hemihydrates.
  • Formulas I and Ia can exist in different solvate forms.
  • chelate refers to the chemical entity formed by the coordination of a compound to a metal ion at two (or more) points.
  • non-covalent complex refers to the chemical entity formed by the interaction of a compound and another molecule wherein a covalent bond is not formed between the compound and the molecule.
  • complexation can occur through van der Waals interactions, hydrogen bonding, and electrostatic interactions (also called ionic bonding).
  • substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, C 1 - 8 alkyl, halogenated Cr 8 alkyl, C 1 - 4 alkoxy, halogenated C 1 - 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Ci- 8 alkylamino, Cr 8 alkylsulphinyl, Cr 8 alkylsulphonyl, C 1 - S alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C 1 - 8 alkyl, halogenated d- 8 alkyl, Cr 4 alkoxy, halogenated Cr 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Crs alkylamino, di-Crs alkylamino, Cr 8 alkylsulphinyl, C 1 - S alkylsulphonyl, Crs alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • R 2 is chosen from C 2 - 4 hydroxyalkyl, C 2 - 4 alkenyl, C 2 - 4 alkenylene-R 5 , C 2 - 4 alkynyl, C 2 - 4 alky
  • X is chosen from -O-, -S- and -NR 9 -;
  • Y is chosen from a covalent bond, -O-, -S- and -NR 9 -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
  • R 3 and R 4 are each independently chosen from:
  • Ci -12 alkyl chosen from mono-, di-, and tri-substituted Ci -I2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, Q ⁇ -alkoxy, halogenated Ci -4 alkoxy, nitro, cyano, carboxy, amino, Ci -4 alkylamino, di-C M -alkylamino, Ci ⁇ -hydroxyalkyl, C 2-4 -hydroxyalkoxy, and Ci -4 - alkylsulphonyl;
  • R 5 is chosen from C 6-I0 aryl, substituted C 6-I0 aryl, Het, substituted Het, C 6-I0 aryl- C 1 - 8 alkyl, substituted C 6-I0 aryl-Ci-g alkyl, Het-Ci- 8 alkyl, and substituted Het-Q-s alkyl, wherein the alkyl portions of C 6- I 0 aryl-Cj-s alkyl and Het- C 1 - 8 alkyl are optionally substituted by oxo and wherein: substituted C 6-I0 aryl is chosen from mono-, di-, and tri-substituted C 6-I0 aryl wherein the substituents are independently chosen from
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substitutents are independently chosen from halo, C 1 - S alkyl, halogenated Ci- 8 alkyl, d- 4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - S alkoxy, amino, Ci- 8 alkylamino, di-Ci- 8 alkylamino, Ci- 8 alkylsulphinyl, Ci-g alkyl sulphonyl, Ci-g alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio;
  • heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Ci- 8 alkyl, halogenated Cp 8 alkyl, Cr 4 alkoxy, halogenated C 1 - 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Ci-g alkylamino, Cj-8 alkylsulphinyl, C 1 -S alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio; and
  • Het is chosen from heterocyclyl, substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl, heteroaryl, and substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl, wherein the substituents are independently chosen from
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Crg alkyl, halogenated Cr 8 alkyl, C 1 - 4 alkoxy, halogenated Cr 4 alkoxy, hydroxy d-8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cj- 8 alkylamino, di-Crs alkylamino, C 1 -S alkylsulphinyl, C 1 -S alkylsulphonyl, d-8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, C 1-8 alkyl, halogenated Cr 8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci - 8 alkylamino, di-Cr 8 alkylamino, Cr 8 alkylsulphinyl, Ci - 8 alkylsulphonyl, d-8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cr 8 alkyl, halogenated Cr 8 alkyl, C1-4 alkoxy, halogenated Cr 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, (Ii-C 1 - S alkylamino, Ci- 8 alkylsulphinyl, Ci- 8 alkylsulphonyl, Q-g alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cr 8 alkyl, halogenated Cp 8 alkyl, C 1 - 4 alkoxy, halogenated C 1 - 4 alkoxy, hydroxy Cj- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Ci- 8 alkylamino, di-Q-salkylamino, C i- 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Ci- 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio, and
  • R 9 is chosen from H, Ci-g alkyl, C 3 - 8 cycloalkyl, substituted Cr 8 alkyl chosen from mono-, di-, and trisubstituted Cp 8 alkyl, and substituted C 3 - 8 cycloalkyl chosen from mono-, di-, and trisubstituted C 3 - S cycloalkyl wherein the substituents are independently chosen from halo, Q- 4 -alkyl, Q- 4 -alkoxy, and oxo; and provided that the compound of Formula I is not chosen from :
  • R 2 is not -O-p-phenylene-0- (CH 2 )phenyl, -O-p-phenylene-NH-C(O)-thiophen-2-yl, -O-(CH 2 ) 2 -O-(phenyl), - NH(CH 2 ) 2 -(4-hydroxyphenyl), -O-(CH 2 ) 2 -(1 ,3-dioxoisoindolin-l -yl), -NH(CH 2 ) 2 -(2-oxo- imidaziolidin-1-yl), -NH(CH 2 ) 2 -(3,4,-dimethoxyphenyl), -S(CH 2 ) 2 - ⁇ henyl, -NH(CH 2 ) 2 - phenyl, -NH(CH 2 ) 2 -morpholin-4-yl, -NH(CH 2 ) 2 -thiophen-2-y
  • R 2 is not -O-p-phenylene-NH-C(O)-3,-4- dimethoxyphenyl, -O-(CH 2 ) 2 -O-(4-methylphenyl), -O-(CH 2 ) 2 -Ophenyl, -O-(CH 2 ) 2 -O(3- bromophenyl), -NH-(CH 2 ) 2 -NH(3-CF 3 -pyridin-2-yl), -S-(CH 2 ) 2 -O-(3-methyl ⁇ henyl), -S- (CH 2 ) 2 -O-(4-Fphenyl), -S-(CH 2 ) 2 -O-(2,4-dichlorophenyl), -NH-(CH 2 ) 2 -NH(4-CF 3 - pyrimidin-2-yl), -NH-(CH 2 ) 2 -O-(3-acet
  • R 2 is not chosen from -O-(CH 2 ) 2 -O-(2- chlorophenyl), -O-(CH 2 ) 2 -O-(4-fluorophenyl), -S-(CH 2 ) 3 - ⁇ henyl, -NH-(CH 2 ) 3 -phenyl, - NH-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 3 -morpholin-4-yl, -NH-(CH 2 ) 3 -(2-oxo-pyrrolidin-l-yl), - NH-(CH 2 ) 2 -mo ⁇ holin-4-yl, -S-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 2 -azepan-l-yl, -S-(CH 2 ) 2 - morpholin-4-yl, -NH-(CH 2 ) 2 ) 2
  • R 1 is 4-chlorophenyl
  • R 2 is not chosen from -O-p-phenylene-O- CH 2 -phenyl, -O-(CH 2 ) 2 -O-(4-methylphenyl), -S-(CH 2 ) 4 -( 1,3-dioxoisoindolin-l-yl), -S- (CH 2 ) 3 -phenyl, -S-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 3 -imidazol-l-yl, -NH-(CH 2 ) 3 -morpholin-4- yl, -NH-(CH 2 ) 2 -morpholin-4-yl, -NH-(CH 2 ) 2 -azepan-l-yl, -S-(CH 2 ) 2 -(1,3- dioxoisoindolin-1-yl), -NH-(CH 2 ) 2 -(3,4
  • R when R is 3-thiophen-2-yl, then R is not chosen from -NH-(CH 2 ) 2 - morpholin-4-yl, -NH-(CH 2 ) 3 -mo ⁇ holin-4-yl, -NH-(CH 2 ) 2 -phenyl, and -NH-(CH 2 ) 2 -(3,4- dimethoxyphenyl) ;
  • R 2 is not chosen from -S-(CH 2 ) 3 - phenyl, -NH-(CH 2 ) 2 -morpholin-4-yl, -NH-(CH 2 ) 2 -C(CH 3 ) 2 -mo ⁇ holin-4-yl, - NHCH 2 C(CH 3 ) 2 -mo ⁇ holin-4-yl, -N(CH 3 )-(CH 2 ) 2 -(3 ,4-dimethoxyphenyl), -S-(CH 2 ) 2 - phenyl, and -NH-(CH 2 ) 2 -(3,4-methylenedioxyphenyl);
  • R 2 is not chosen from -O-p- phenylene-O-CH 2 -phenyl, -S-(CH 2 ) 3 -phenyl, -S-(CH 2 ) 3 -mo ⁇ holin-4-yl, -S-(CH 2 ) 2 - phenyl, -NH-(CH 2 ) 3 -(4-hydroxy ⁇ henyl), -O-(CH 2 ) 2 -(l,3-dioxoisoindolin-l-yl), -NH- (CH 2 ) 2 - mo ⁇ holin-4-yl, -NH-(CH 2 ) 3 - mo ⁇ holin-4-yl, -NH-(CH 2 ) 2 -(3,4- methylenedioxyphenyl), -S-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 2 -phenyl, -NH-(CH 2 ) 2 -phenyl, -NH
  • R 2 is not chosen from -(CH 2 ) 3 -phenyl, -S- (CH 2 ) 3 -morpholin-4-yl, -S-(CH 2 ) 2 -phenyl, -S-(CH 2 ) 3 -phenyl, -NH-(CH 2 ) 3 -morpholin-4- yl, -NH-(CH 2 ) 3 -(2-oxo-pyrrolidin-l-yl), and -NH-(CH 2 ) 2 -(3,4-ethylenedioxyphenyl); and
  • R 2 is not -NH-(CH 2 ) 2 -(3,4- ethylenedioxyphenyl);
  • R 1 is chosen from aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, and substituted heteroaryl. [045] In certain embodiments, R 1 is chosen from aryl and substituted aryl wherein substituted aryl is chosen from mono-, di-, and tri-substituted aryl. In certain embodiments, R 1 is chosen from phenyl and substituted phenyl wherein substituted phenyl is chosen from mono-, di-, and tri-substituted phenyl.
  • R 1 is chosen from phenyl and phenyl substituted with one or more (such as one, two, or three) halo (such as fluoro). In some embodiments, R 1 is chosen from phenyl and 4-F- phenyl.
  • R 1 is chosen from heterocyclyl, substituted heterocyclyl, heteroaryl, and substituted heteroaryl.
  • R 1 is chosen from optionally substituted furanyl (e.g., optionally substituted furan-2-yl), optionally substituted pyridinyl (e.g., pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, any of which is optionally substituted), optionally substituted pyrimidinyl (e.g., optionally substituted 5- pyrimidin-2-yl), optionally substituted pyrazinyl (e.g., optionally substituted 5-pyrazin-2- yl), and optionally substituted thiazolyl (e.g., optionally substituted l,3-thiazol-2-yl).
  • R 1 is chosen from optionally substituted furanyl
  • R 1 is chosen from 5-chloro-furan-2-yl, furan-2-yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, l,3-thiazol-2-yl, 5-pyrimidin-2-yl and 5-pyrazin-
  • R 1 is chosen from furan-2-yl, pyridin-4-yl, pyridin-3-yl, l,3-thiazol-2-yl, and 5-pyrimidin-2-yl.
  • R 2 is chosen from -O-arylene-Y-R 5 , -
  • R 2 is -O-arylene-Y-R 5 . In certain embodiments,
  • R 2 is -O-phenylene-Y-R 5 .
  • the -O and -YR 5 substituents attached the phenylene group are meta-substituted.
  • R 2 is -X(CR 3 R 4 ) n Y-R 5 .
  • R 2 is -(CR 6 R 7 ) m YR 5 .
  • X is -NH-. In certain embodiments, X is -O-.
  • Y is a bond. In certain embodiments, Y is -O-.
  • Y is -NH-.
  • n is chosen from 3, 4, and 5. In certain embodiments, n is chosen from 2 and 3. In certain embodiments, n is 3. In certain embodiments, n is 2.
  • m is chosen from 3, 4, and 5.
  • R 3 and R 4 are independently chosen from H
  • R 3 and R 4 are H.
  • R 6 and R 7 are independently chosen from H, CH 3 , and F. In certain embodiments, R 6 and R 7 are H;
  • -(CR 6 R 7 ) m Y-R 5 is -(CH 2 ) 4 -O-R 5 .
  • -X(CR 3 R 4 ) n Y-R 5 is chosen from -NH-(CH 2 ) 3 -O-
  • R 5 -NH-(CH 2 ) 2 -NH-R 5 , -O-(CH 2 ) 2 -O-R 5 , -O-(CH 2 ) 3 -O-R 5 , -O-CH 2 -C(CH 3 ) 2 -CH 2 -O-R 5 , and-NH-(CH 2 ) 2 -O-R 5 .
  • -X(CR 3 R 4 ) n Y-R 5 is chosen from -NH-
  • -X(CR 3 R 4 ) ⁇ Y-R 5 is chosen from -NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5 .
  • -X(CR 3 R 4 ) n YR 5 is -NH-(CH 2 ) 3 -R 5 (i.e. Y is a covalent bond).
  • R 9 is chosen from H, C 1 - 4 alkyl; and C 3 - 8 cycloalkyl, substituted C)- 4 alkyl chosen from mono-, di-, and tri-substituted Cr 4 alkyl; and substituted C 3 - 8 cycloalkyl chosen from mono-, di-, and tri-substituted C 3 - 8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci- 4 -alkyl, Ci- 4 -alkoxy, and oxo.
  • R 5 is chosen from pyridin-3-yl and pyridin-4-yl, each of which is optionally substituted with one or two groups chosen from
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cp 8 alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cp 8 alkyl, halogenated Cp 8 alkyl, Cp 4 alkoxy, halogenated Cp 4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cp 8 alkylamino, Cp 8 alkylsulphinyl, Cp 8 alkylsulphonyl, Cp 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • R 5 is chosen from pyridin-2-yl and pyridin-4-yl, each of which is substituted with one or two groups chosen from (1) Cp 8 alkyl, (2) C 3 - 8 cycloalkyl,
  • substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C 1 - S alkyl, halogenated Ci- 8 alkyl, C]- 4 alkoxy, halogenated Q- 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, d- 8 alkylamino, di-Cj-g alkylamino, C 1 ⁇ alkylsulphinyl, Cp 8 alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, d- 8 alkyl, halogenated Crs alkyl, Cj-4 alkoxy, halogenated Cr 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cr 8 alkylamino, di-Crs alkylamino, C r8 alkylsulphinyl, Cr 8 alkylsulphonyl, Q-s alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio,
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cr 8 alkyl, halogenated Cj- 8 alkyl, Cr 4 alkoxy, halogenated Q- 4 alkoxy, hydroxy Cr 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Q-8 alkylamino, di-Crs alkylamino, C 1 -S alkylsulphinyl, Crs alkylsulphonyl, Cr 8 alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Ci- 8 alkyl, halogenated Cr 8 alkyl, Cj- 4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C 2 - 8 alkoxy, amino, Cp 8 alkylamino, di-Cr 8 alkylamino, Ci-g alkylsulphinyl, d- 8 alkylsulphonyl, C 1 - S alkylsulphonamido, -COR 9 , -CSR 9 , -SR 9 , cyano, hydroxyl, nitro, oxo, and thio.
  • substituents are independently chosen from halo,Ci- 8 alkyl, halogenated Cr 8 alkyl, Cj- 4 alkoxy, halogenated Ci- 4 alkoxy
  • R 1 is chosen from aryl (such as phenyl), substituted aryl chosen from mono-, di-, and trisubstituted aryl wherein the substitutent is halo (such as F, e.g., phenyl substituted by fluoro), heterocyclyl, and substituted heterocyclyl, such as optionally substituted heterocyclyl chosen from optionally substituted furanyl, optionally substituted pyridinyl, optionally substituted pyrimidinyl, optionally substituted pyrazinyl, and optionally substituted thiazolyl, such as heterocyclyl chosen from 5-chloro-furan-2- yl, furan-2-yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, l,3-thiazol-2-yl, 5- pyrazin-2-yl, and 5-pyrimidin-2-yl; R 2 is -X(CR 3 R 4 ) n Y-
  • -X(CR 3 R 4 ) n Y- is chosen from -NH-(CH 2 ) 3 -O-, -NH-(CH 2 ) 2 -NH-, -O-(CH 2 ) 2 -O-, -O- (CHb) 3 -O-, -O-CH 2 -C(CH 3 ) 2 -CH 2 -O-, and -NH-(CH 2 ) 2 -O-, and in certain embodiments, -X(CR 3 R 4 ) n Y- is chosen from -NH-(CH 2 ) 3 -O- and -O-(CH 2 ) 3 -O-; R 3 , R 4 , R 6 , and R 7 are independently chosen from H, CH 3 , and F, and in certain embodiments, R 3 , R 4 , R 6 , and R 7 are H;
  • X is chosen from O and NR 9 , and in certain embodiments, X is chosen from O and NH, Y is chosen from O and NR 9 , and in certain embodiments, Y is chosen from O and NH, and in yet other certain embodiments, Y is O; and R 5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- and cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH-CO-NH-,
  • substituted benzoyl chosen from mono-, di-, and tri-substituted benzoyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- and cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH-CO-NH-, NH 2 -CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl) 2 N-CO-, NH 2 -alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl) 2 N-alkylene-NH-CO-, NH 2 -alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO 2 -NH-, (
  • R 1 is chosen from aryl (such as phenyl), aryl (such as phenyl) substituted by halo (such as F), and heterocyclyl, substituted heterocyclyl, such as optionally substituted heterocyclyl chosen from optionally substituted furanyl, optionally substituted pyridinyl, and optionally substituted thiazolyl, such as heterocyclyl chosen from fi ⁇ ran-2- yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, and l,3-thiazol-2-yl;
  • R 2 is -X(CR 3 R 4 ) n Y-R 5 ;
  • -X(CR 3 R 4 ) n Y- is chosen from -NH-(CH 2 ) 3 -O-, -NH-(CH 2 ) 2 -NH-, -O-(CH 2 ) 3 -O-, and-NH- (CH 2 ) 2 -O-, and in certain embodiments, -X(CR 3 R 4 ) n Y- is chosen from -NH- (CH 2 ) 3 -O- and -O-(CH 2 ) 3 -O-;
  • R 3 , R 4 , R 6 , and R 7 are independently chosen from H, CH 3 , and F, and in certain embodiments, R 3 , R 4 , R 6 , and R 7 are H;
  • X is chosen from O and NR 9 , and in certain embodiments, X is chosen from O and NH;
  • Y is chosen from O and NR 9 , and in certain embodiments, Y is chosen from O and NH, and in yet other certain embodiments, Y is O; the subscript n is chosen from 2 and 3, and in certain embodiments, the subscript n is 3;
  • R 5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- and cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH-CO-NH-, NH 2 -CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl) 2 N-CO-, NH 2 -alkylene-NH-CO-, (alkyl)NH-alkylene
  • R 1 is chosen from phenyl, furanyl, pyridinyl, pyrimidinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, substituted pyrimidinyl, and substituted thiazolyl, and R 2 is -X(CR 3 R 4 ) n YR 5 .
  • R 1 is chosen from furanyl, pyridinyl, thiazolyl, phenyl, and phenyl substituted by halo, and R 2 is -X(CR 3 R 4 ) n YR 5 .
  • R 1 is chosen from phenyl and phenyl substituted by halo, and R 2 is -X(CR 3 R 4 ) n YR 5 .
  • R 1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl
  • R 2 is chosen from -NH-(CH 2 ) 3 -O-R 5 , -NH-(CH 2 ) 2 -NH-R 5 , -O-(CH 2 ) 3 -O-R 5 , and -NH-(CH 2 ) 2 -O-R 5 .
  • R 1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl
  • R 2 is chosen from -NH-(CH 2 ) 3 -O-R 5 , -NH- (CH 2 ) 2 -NH-R 5 , -O-(CH 2 ) 3 -O-R 5 , and -NH-(CH 2 ) 2 -O-R 5 .
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is chosen from -NH-(CH 2 ) 3 -O-R 5 , -NH-(CH 2 ) 2 -NH-R 5 , -O-(CH 2 ) 3 -O-R 5 , and -NH-(CH 2 ) 2 -O-R 5 .
  • R 1 is chosen from phenyl, furanyl, pyridinyl, or thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5
  • R 1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5 .
  • R 1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, which in each case is unsubstituted or substituted, and R is -O-(CH 2 ) 3 -O-R .
  • R 1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R 2 is -O-(CH 2 ) 3 -O-R 5 .
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is -O-(CH 2 ) 3 -O-R 5 .
  • R 1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substitutedthiazolyl;
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5 ;
  • R 5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
  • R 1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl;
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-
  • R 5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, and pyridinyl,substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 or -O-(CH 2 ) 3 -O-R 5
  • R 5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
  • R 1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl;
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5 , and
  • R 5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g.,
  • R 1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl;
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O- (CH 2 ) 3 -O-R 5 , and R 5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- or cyclopropyl-CO- NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH-CO-NH-, NH 2 - CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl) 2 N-CO-, NH 2 -alkyl
  • R 1 is chosen from phenyl and phenyl substituted by halo;
  • R 2 is chosen from NH-(CH 2 ) 3 -O-R 5 and -O-(CH 2 ) 3 -O-R 5 ; and
  • R 5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH 3 -CO- NH-, CH 3 CH 2 -CO-NH- or cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH-CO-NH-, NH 2 -CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH- CO-, (alkyl) 2 N-CO-, NH 2 -alkylene-NH-CO-, (alkyl)NH-alkylene
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is NH-(CH 2 ) 2 -O-R 5
  • R 5 is chosen from phenyl and phenyl substituted by alkyl-CO-NH-.
  • R 1 is chosen from phenyl and phenyl substituted by halo
  • R 2 is NH-(CH 2 ) 3 -O-R 5
  • R 5 is chosen from benzyl, benzoyly, and benzothiadiazolyl, substituted benzyl, substituted benzoyly, and substituted benzothiadiazolyl, which in each case the substituents are chosen from nitro, alkoxy (e.g., methoxy), cyano, halogenated alkoxy (e.g., OCF 3) , alkoxycarbonyl (e.g., methoxycarbonyl), and alkyl-CO-NH- (e.g., acetamido).
  • R 1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl;
  • R is -O-(CH 2 ) 3 -O-R , and R is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- or cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH- CO-NH-, NH 2 -CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl) 2 N-CO-, NH 2 - alkylene-
  • R 1 is chosen from phenyl and halogenated phenyl;
  • R 2 is -O-(CH 2 ) 3 -O-R 5 , and
  • R 5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH 3 -CO-NH-, CH 3 CH 2 -CO-NH- or cyclopropyl-CO-NH-), NH 2 -CO-NH-, alkyl-NH-CO-NH-, NH 2 -NH-CO-NH-, OH-NH- CO-NH-, NH 2 -CO-, NH 2 -NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl) 2 N-CO-, NH 2 - alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl) 2 N-alkylene-NH-CO-CO-,
  • the compound of Formula I is chosen from:
  • the compound of Formula I is chosen from:
  • the compound of Formula I is chosen from those listed in Table I:
  • compositions comprising at least one chemical entity described herein and a pharmaceutically acceptable carrier and, optionally, another active agent as discussed below.
  • a method of inhibiting a PDElO enzyme e.g., as determined by a conventional assay or one described herein, either in vitro or in vivo (in an animal, e.g., in an animal model, or in a mammal or in a human); a method of treating a psychiatric or neurological syndrome, e.g., pyschoses, obsessive-compulsive disorder and/or Parkinson's disease; a method of treating a disease state modulated by PDElO activity, in a mammal, e.g., a human, e.g., those disease states mentioned herein.
  • Methods include, but are not limited to, methods of enhancing cognition in a patient in whom such enhancement is desired, methods of treating a patient suffering from cognition impairment or decline, methods of treating a patient having a disease involving decreased cAMP and/or cGMP levels, methods of inhibiting PDElO enzyme activity in a patient, methods of treating a patient suffering psychoses, in particular schizophrenia or bipolar disorder, methods of treating a patient suffering from obsessive- compulsive disorder, methods of treating a patient suffering from Parkinson's disease.
  • the disease state is one that involves elevated intracellular PDElO levels or decreased cAMP and/or cGMP levels, e.g., involving neurological or psychiatric syndromes, especially those states associated with psychoses, most especially schizophrenia or bipolar disorder, obsessive-compulsive disorder, and/or Parkinson's disease. All methods comprise administering to the patient an effective amount of at least one chemical entity described herein. In certain embodiments, the patient is human. [096] Chemical entities described herein can be made by the methods depicted in the reaction schemes shown below.
  • the starting materials and reagents used in preparing these compounds are either available from commercial suppliers such as Aldrich Chemical Co., (Milwaukee, Wis.), Bachem (Torrance, Calif.), or Sigma (St. Louis, Mo.) or are prepared by methods known to those skilled in the art following procedures set forth in references such as Fieser and Fieser's Reagents for Organic Synthesis, Volumes 1-17 (John Wiley and Sons, 1994); Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989); Organic Reactions, Volumes 1-40 (John Wiley and Sons, 2003), March's Advanced Organic Chemistry, (John Wiley and Sons, 4th Edition) and Larock's Comprehensive Organic Transformations (VCH Publishers Inc., 1989).
  • the reactions described herein take place at atmospheric pressure over a temperature range from about -78 0 C to about 150 0 C, such as from about 0 0 C to about 125 0 C, for example, at about room (or ambient) temperature, e.g., about 20 0 C.
  • compound 4 can be first converted to a compound of formula 5 by treating with with a chlorinating agent such as thionyl chloride and the.
  • Compound 5 is then converted to a compound of Formula I by treating it with is HO(CR 3 R 4 ) n YR 5 , is HS(CR 3 R 4 ) n YR 5 , or is NH 2 (CR 3 R 4 ) n YR 5 under conditions well known in the art. Detailed descriptions of such procedures are provided in the Working examples below.
  • Compounds of Formula I wherein R 2 is -(CR 3 R 4 ) n YR 5 can be prepared by reaction of compound 5 with the Grignard BrMg-(CR 3 R 4 ),, YR 5 under anhydrous conditions in a polar aprotic solvent (such as THF) using CuI and/or FeBr 2 as catalysts.
  • the optical isomers of compounds of Formula I can be obtained by resolution of the racemic mixtures according to conventional processes, for example, by the formation of diastereomeric salts using an optically active acid or base or formation of covalent diastereomers.
  • Examples of appropriate acids are tartaric, diacetyltartaric, dibenzoyltartaric, ditoluoyltartaric and camphorsulfonic acid.
  • Mixtures of diastereomers can be separated into their individual diastereomers on the basis of their physical and/or chemical differences by methods known to those skilled in the art, for example, by chromatography or fractional crystallization.
  • the optically active bases or acids are then liberated from the separated diastereomeric salts.
  • a different process for separation of optical isomers involves the use of chiral chromatography (e.g., chiral HPLC columns), with or without conventional derivation, optimally chosen to maximize the separation of the enantiomers.
  • Suitable chiral HPLC columns are manufactured by Diacel, e.g., Chiracel OD and Chiracel OJ among many others, all routinely selectable.
  • Enzymatic separations, with or without derivitization, are also useful.
  • the optically active compounds of Formula I can likewise be obtained by utilizing optically active starting materials in chiral syntheses processes under reaction conditions which do not cause racemization.
  • the compounds can be used in different enriched isotopic forms, e.g., enriched in the content of 2 H, 3 H, 11 C, 13 C and/or 14 C.
  • the compounds are deuterated.
  • Such deuterated forms can be made by the procedure described in U.S. Patent Nos. 5,846,514 and 6,334,997.
  • deuteration can improve the efficacy and increase the duration of action of drugs.
  • Deuterium substituted compounds can be synthesized using various methods such as described in: Dean, Dennis C; Editor. Recent Advances in the Synthesis and Applications of Radiolabeled Compounds for Drug Discovery and Development. [In: Curr., Pharm. Des., 2000; 6(10)] (2000), 110 pp. CAN 133:68895 AN 2000:473538 CAPLUS; Kabalka, George W.; Varma, Rajender S. The Synthesis of Radiolabeled Compounds via Organometallic Intermediates. Tetrahedron (1989), 45(21), 6601-21, CODEN: TETRAB ISSN:0040-4020. CAN 112:20527 AN 1990:20527 CAPLUS; and Evans, E. Anthony. Synthesis of radiolabeled compounds, J. Radioanal. Chem. (1981), 64(1-2), 9-32. CODEN: JRACBN ISSN:0022-4081, CAN 95:76229 AN 1981 :476229 CAPLUS.
  • the acid addition salts of the claimed compounds may be prepared by reaction of the compounds with the appropriate inorganic or organic acid via any of a number of known methods.
  • alkali and alkaline earth metal salts are prepared by reacting the chemical entities described herein with the appropriate base via a variety of known methods.
  • the salts formed are pharmaceutically acceptable for administration to mammals.
  • pharmaceutically unacceptable salts of the compounds are suitable as intermediates, for example, for isolating the compound as a salt and then converting the salt back to the free base compound by treatment with an alkaline reagent. The free base can then, if desired, be converted to a pharmaceutically acceptable acid addition salt.
  • compositions comprising at least one chemical entity described herein and one or more pharmaceutically acceptable carriers.
  • the chemical entities described herein can be administered to anyone requiring PDElO inhibition. Administration may be accomplished according to patient needs, for example, orally, nasally, parenterally (subcutaneously, intravenously, intramuscularly, intrasternally and by infusion) by inhalation, rectally, vaginally, topically and by ocular administration.
  • Various solid oral dosage forms can be used for administering chemical entities described herein including such solid forms as tablets, gelcaps, capsules, caplets, granules, lozenges and bulk powders.
  • the chemical entities described herein can be administered alone or combined with various pharmaceutically acceptable carriers, diluents (such as sucrose, mannitol, lactose, starches) and excipients known in the art, including but not limited to suspending agents, solubilizers, buffering agents, binders, disintegrants, preservatives, colorants, flavorants, lubricants and the like.
  • diluents such as sucrose, mannitol, lactose, starches
  • excipients known in the art, including but not limited to suspending agents, solubilizers, buffering agents, binders, disintegrants, preservatives, colorants, flavorants, lubricants and the like.
  • Time release ⁇ capsules, tablets and gels are also advantageous in administering the chemical entities described herein.
  • liquid oral dosage forms can also be used for administering chemical entities described herein, including aqueous and non-aqueous solutions, emulsions, suspensions, syrups, and elixirs.
  • Such dosage forms can also contain suitable inert diluents known in the art such as water and suitable excipients known in the art such as preservatives, wetting agents, sweeteners, flavorants, as well as agents for emulsifying and/or suspending the chemical entities described herein.
  • the chemical entities described herein may be injected, for example, intravenously, in the form of an isotonic sterile solution. Other preparations are also possible.
  • Suppositories for rectal administration of the chemical entities described herein can be prepared by mixing the compound with a suitable excipient such as cocoa butter, salicylates and polyethylene glycols.
  • Formulations for vaginal administration can be in the form of a pessary, tampon, cream, gel, paste, foam, or spray formula containing, in addition to the active ingredient, such suitable carriers as are known in the art.
  • the pharmaceutical composition can be in the form of creams, ointments, liniments, lotions, emulsions, suspensions, gels, solutions, pastes, powders, sprays, and drops suitable for administration to the skin, eye, ear or nose. Topical administration may also involve transdermal administration via means such as transdermal patches.
  • Aerosol formulations suitable for administering via inhalation also can be made.
  • the chemical entities described herein can be administered by inhalation in the form of a powder (e.g., micronized) or in the form of atomized solutions or suspensions.
  • the aerosol formulation can be placed into a pressurized acceptable propellant.
  • the chemical entities described herein can be administered as the sole active agent or in combination with other pharmaceutical agents such as other agents used in the treatment of psychoses, such as schizophrenia and bipolar disorder, obsessive- compulsive disorder, Parkinson's disease, cognitive impairment and/or memory loss, e.g., nicotinic ⁇ -7 agonists, PDE4 inhibitors, other PDElO inhibitors, calcium channel blockers, muscarinic ml and m2 modulators, adenosine receptor modulators, ampakines, NMDA-R modulators, mGluR modulators, dopamine modulators, serotonin modulators, canabinoid modulators, and cholinesterase inhibitors (e.g., donepezil, rivastigimine, and galanthanamine).
  • each active ingredient can be administered either in accordance with their usual dosage range or a dose below their usual dosage range.
  • the chemical entities described herein can be administered in combination with other pharmaceutical agents used in the treatment of schizophrenia, e.g., Clozaril, Zyprexa, Risperidone, and Seroquel.
  • other pharmaceutical agents used in the treatment of schizophrenia e.g., Clozaril, Zyprexa, Risperidone, and Seroquel.
  • methods for treating schizophrenia, including memory impairment associated with schizophrenia comprising administering to a patient, simultaneously or sequentially, at least one chemical entities described herein and another agent used in the treatment of schizophrenia such as Clozaril, Zyprexa, Risperidone, and Seroquel.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of schizophrenia, e.g., Clozaril, Zyprexa, Risperidone, and Seroquel.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of schizophrenia e.g., Clozaril, Zyprexa, Risperidone, and Seroquel.
  • the chemical entities described herein can be administered in combination with other pharmaceutical agents used in the treatment bipolar disorder such as Lithium, Zyprexa, or Depakote.
  • compositions comprising a at least one chemical entity described herein and another pharmaceutical agent used in the treatment of bipolar disorder such as Lithium, Zyprexa, or Depakote.
  • methods for treating Parkinson's disease including treating memory and/or cognitive impairment associated with Parkinson's disease, comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described hereinand another agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described hereinand another pharmaceutical agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
  • kits comprising a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
  • methods for treating memory and/or cognitive impairment associated with Alzheimer's disease comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described hereinand another agent used in the treatment of Alzheimer's disease such as Reminyl, Cognex, Aricept, Exelon, Akatinol, Neotropin, Eldepryl, Estrogen and Cliquinol.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of Alzheimer's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Alzheimer's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone.
  • Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone.
  • Also provided are methods for treating memory and/or cognitive impairment associated with dementia comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon.
  • Also provided are methods for treating memory and/or cognitive impairment associated with epilepsy comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol.
  • Also provided are methods for treating memory and/or cognitive impairment associated with multiple sclerosis comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone.
  • the agents can be present in a combined composition or can be administered separately.
  • compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone.
  • kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone.
  • PDElO inhibitors will raise the levels of cAMP or cGMP within cells that express PDElO. Inhibition of PDElO enzyme activity may be of relevance to diseases caused by deficient amounts of c AMP or cGMP in cells. Alternatively, PDElO inhibitors may be of benefit in cases wherein raising the amount of cAMP or cGMP above normal levels results in a therapeutic effect. Inhibitors of PDElO may be used to treat disorders of the peripheral and central nervous system, cardiovascular diseases, cancer, gastroenterological diseases, endocrinological diseases and urological diseases.
  • PDElO enzymes in animals, e.g., mammals, for example, humans, wherein such inhibition has a therapeutic effect, such as where such inhibition may relieve conditions involving neurological or psychiatric syndromes, such as the loss of memory or psychoses.
  • Such methods comprise administering to an animal in need thereof, such as a mammal, for example, a human, an inhibitory amount of at least one chemical entity described herein , alone or as part of a formulation.
  • Indications that may be treated with PDE 10 inhibitors, either alone or in combination with other drugs, include, but are not limited to, those diseases thought to be mediated in part by the basal ganglia, prefrontal cortex and hippocampus. These indications include psychoses, Parkinson's disease, dementias, obsessive compulsion disorder, tardive dyskinesia, choreas, depression, mood disorders, impulsivity, drug addiction, attention deficit/hyperactivity disorder (ADHD), depression with parkinsonian states, personality changes with caudate or putamen disease, dementia and mania with caudate and pallidal diseases, and compulsions with pallidal disease. [0128] Psychoses are disorders that affect an individual's perception of reality.
  • Psychoses are characterized by delusions and hallucinations. Also provided are methods for treating patients suffering from all forms of psychoses, including but not limited to schizophrenia, late-onset schizophrenia, schizoaffective disorders, prodromal schizophrenia, and bipolar disorders. Treatment may be for the positive symptoms of schizophrenia as well as for the cognitive deficits and negative symptoms.
  • Other indications for PDElO inhibitors are psychoses resulting from drug abuse (including amphetamines and PCP), encephalitis, alcoholism, epilepsy, Lupus, sarcoidosis, brain tumors, multiple sclerosis, dementia with Lewy bodies, or hypoglycemia.
  • Other psychiatric disorders like posttraumatic stress disorder (PTSD), and schizoid personality may also be treated with PDElO inhibitors.
  • Obsessive-compulsive disorder has been linked to deficits in the frontal-striatal neuronal pathways.
  • OCD Obsessive-compulsive disorder
  • PDElO inhibitors cause cAMP to be elevated in these neurons; elevations in cAMP result in an increase in CREB phosphorylation and thereby improve the functional state of these neurons.
  • PDElO inhibitors should be useful for the indication of OCD.
  • OCD may result, in some cases, from streptococcal infections that cause autoimmune reactions in the basal ganglia (Giedd J. N.
  • PDElO inhibitors may serve a neuroprotective role, administration of PDElO inhibitors may prevent the damage to the basal ganglia after repeated streptococcal infections and thereby prevent the development of OCD.
  • the level of cAMP or cGMP within neurons is believed to be related to the quality of memory, such as long term memory.
  • the level of this enzyme affects memory in animals, for example, in humans.
  • a compound that inhibits cAMP phosphodiesterase (PDE) can thereby increase intracellular levels of cAMP, which in turn activate a protein kinase that phosphorylates a transcription factor (cAMP response binding protein), which transcription factor then binds to a DNA promoter sequence to activate genes that are important in long term memory.
  • PDE cAMP phosphodiesterase
  • cAMP response binding protein a transcription factor response binding protein
  • Dementias are diseases that include memory loss and additional intellectual impairment separate from memory. Also provided are methods for treating patients suffering from memory impairment in all forms of dementia. Dementias are classified according to their cause and include: neurodegenerative dementias (e.g., Alzheimer's, Parkinson's disease, Huntingdon's disease, Pick's disease), vascular (e.g., infarcts, hemorrhage, cardiac disorders), mixed vascular and Alzheimer's, bacterial meningitis, Creutzfeld-Jacob Disease, multiple sclerosis, traumatic (e.g., subdural hematoma or traumatic brain injury), infectious (e.g., HIV), genetic (down syndrome), toxic (e.g., heavy metals, alcohol, some medications), metabolic (e.g., vitamin B12 or folate deficiency), CNS hypoxia, Cushing's disease, psychiatric (e.g., depression and schizophrenia), and
  • neurodegenerative dementias e.g., Alzheimer's, Parkinson's disease, Hunt
  • the condition of memory impairment is manifested by impairment of the ability to learn new information and/or the inability to recall previously learned information.
  • methods for dealing with memory loss separate from dementia including mild cognitive impairment (MCI) and age-related cognitive decline.
  • MCI mild cognitive impairment
  • methods of treatment for memory impairment as a result of disease are also provided.
  • Memory impairment is a primary symptom of dementia and can also be a symptom associated with such diseases as Alzheimer's disease, schizophrenia, Parkinson's disease, Huntington's disease, Pick's disease, Creutzfeld-Jakob disease, HIV, cardiovascular disease, and head trauma as well as age-related cognitive decline.
  • ALS amylolaterosclerosis
  • MSA multiple systems atrophy
  • schizophrenia Parkinson's disease, Huntington's disease, Pick's disease, Creutzfeld-Jakob disease, depression, aging, head trauma, stroke, spinal cord injury, CNS hypoxia, cerebral senility, diabetes associated cognitive impairment, memory deficits from early exposure of anesthetic agents, multiinfarct dementia and other neurological conditions including acute neuronal diseases, as well as HIV and cardiovascular diseases.
  • agents and/or methods to stimulate the formation of memory in "normal" subjects i.e., subjects who do not exhibit an abnormal or pathological decrease in a memory function
  • "normal" subjects i.e., subjects who do not exhibit an abnormal or pathological decrease in a memory function
  • the chemical entities described herein are used in the treatment of a class of disorders known as polyglutamine-repeat diseases. These diseases share a common pathogenic mutation.
  • the expansion of a CAG repeat, which encodes the amino acid glutamine, within the genome leads to production of a mutant protein having an expanded polyglutamine region.
  • Huntington's disease has been linked to a mutation of the protein huntingtin.
  • huntingtin In individuals who do not have Huntington's disease, huntingtin has a polyglutamine region containing about 8 to 31 glutamine residues. For individuals who have Huntington's disease, huntingtin has a polyglutamine region with over 37 glutamine residues.
  • Huntington's disease other known polyglutamine-repeat diseases and the associated proteins are: dentatorubral-pallidoluysian atrophy, DRPLA (atrophin-1); spinocerebellar ataxia type-1 (ataxin-1); spinocerebellar ataxia type-2 (ataxin-2); spinocerebellar ataxia type-3 also called Machado-Joseph disease, MJD (ataxin-3); spinocerebellar ataxia type-6 (alpha la- voltage dependent calcium channel); spinocerebellar ataxia type-7 (ataxin-7); and spinal and bulbar muscular atrophy, SBMA, also know as Kennedy disease (androgen receptor).
  • DRPLA dentator
  • a method of treating a polyglutamine-repeat disease or CAG repeat expansion disease comprising administering to a patient, such as a human, a therapeutically effective amount of a compound.
  • HD Huntington's disease
  • DRPLA dentatorubral-pallidoluysian atrophy
  • spinocerebellar ataxia type-1 spinocerebellar ataxia type-2
  • the basal ganglia are important for regulating the function of motor neurons; disorders of the basal ganglia result in movement disorders. Most prominent among the movement disorders related to basal ganglia function is Parkinson's disease (Obeso JA et al., Neurology, 2004 Jan 13;62(1 Suppl l):S17-30). Other movement disorders related to dysfunction of the basla ganglia include tardive dyskinesia, progressive supranuclear palsy and cerebral palsy, corticobasal degeneration, multiple system atrophy, Wilson disease, and dystonia, tics, and chorea. In certain embodiments, PDElO inhibitors may be used to treat movement disorders related to dysfunction of basal ganglia neurons.
  • PDEl 0 inhibitors can be used to raise cAMP or cGMP levels and prevent neurons from undergoing apoptosis.
  • PDElO inhibitors may be anti-inflammatory by raising cAMP in glial cells.
  • ALS amylolaterosclerosis
  • MSA multiple systems atrophy
  • PDElO inhibitors may be used to stop disease progression or restore damaged circuits in the brain by a combination of effects including increased synaptic plasticity, neurogenesis, anti-inflammatory, nerve cell regeneration and decreased apoptosis
  • cAMP cyclopentadiene
  • cGMP cyclopentadiene
  • cells may become cancerous by expressing PDElO and reducing the amount of cAMP or cGMP within cells.
  • inhibition of PDElO activity will inhibit cell growth by raising cAMP (US20040138249, Pyrrolo (2.1a)dihydroisoquinolines and their use as phosphodiesterase 10a inhibitors).
  • PDElO may be expressed in the transformed, cancerous cell but not in the parent cell line.
  • PDElO is expressed and PDElO inhibitors reduce the growth rate of the cells in culture.
  • breast cancer cells are inhibited by administration of PDElO inhibitors.
  • Many other types of cancer cells may also be sensitive to growth arrest by inhibition of PDElO. Therefore, chemical entities described herein may be used to stop the growth of cancer cells that express PDElO.
  • the chemical entities described herein are also suitable for use in the treatment of diabetes and related disorders such as obesity, by focusing on regulation of the cAMP signaling system. By inhibiting PDE-IOA activity, intracellular levels of cAMP and increased, thereby increasing the release of insulin-containing secretory granules and, therefore, increasing insulin secretion. See, e.g., WO 2005/012485.
  • a method of treating diabetes and related disorders comprising administering to a patient, such as a mammal, such as a human, a therapeutically effective amount of at least one chemical entity described herein.
  • a method of treating type 1 diabetes, type 2 diabetes, Syndrome X, impaired glucose tolerance, impaired fasting glucose, gestational diabetes, maturity-onset diabetes of the young (MODY), latent autoimmune diabetes adult (LADA), associated diabetic dyslipidemia, hyperglycemia, hyperinsulinemia, dyslipidemia, hypertriglyceridemia, obesity and insulin resistance comprising administering to a patient, such as a mammal, such as a human, a therapeutically effective amount of at least one chemical entity described herein.
  • the chemical entities described herein may also be administered in combination with other known therapies for the treatment of diabetes, including, but not limited to, PPAR ligands (e.g. agonists, antagonists, such as Rosiglitazone, Troglitazone and Pioglitazone), insulin secretagogues (for example, sulfonylurea drugs (such as Glyburide, Glimepiride, Chlorpropamide, Tolbutamide, and Glipizide) and non-sulfonyl secretagogues), D-glucosidase inhibitors (such as Acarbose, Miglitol, and Voglibose), insulin sensitizers (such as the PPAR-D agonists, e.g., the glitazones; biguanides, PTP- IB inhibitors, DPP-IV inhibitors and 1 lbeta-HSD inhibitors), hepatic glucose output lowering compounds (such as glucagon antagonists and metaform
  • the dosages of the chemical entities described herein depend upon a variety of factors including the particular syndrome to be treated, the severity of the symptoms, the route of administration, the frequency of the dosage interval, the particular compound utilized, the efficacy, toxicology profile, pharmacokinetic profile of the compound, and the presence of any deleterious side-effects, among other considerations.
  • the chemical entities described herein are typically administered at dosage levels and in a mammal customary for PDElO inhibitors such as those known compounds mentioned above.
  • the compounds can be administered, in single or multiple doses, by oral administration at a dosage level of generally 0.001-100 mg/kg/day, for example, 0.01-100 mg/kg/day, such as 0.1-70 mg/kg/day, for example, 0.5-10 mg/kg/day.
  • Unit dosage forms can contain generally 0.01-1000 mg of active compound, for example, 0.1-50 mg of active compound.
  • the compounds can be administered, in single or multiple dosages, at a dosage level of, for example, 0.001-50 mg/kg/day, such as 0.001-10 mg/kg/day, for example, 0.01-1 mg/kg/day.
  • Unit dosage forms can contain, for example, 0.1-10 mg of active compound.

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Abstract

Certain thienopyrimidine derivatives are useful for the inhibition of PDE10 enzymes, and thus are useful for treating psychiatric or neurological syndromes, e.g., pyschoses, obsessive-compulsive disorder and/or Parkinson's disease, as well as, for example, treating a disease state modulated by PDE10 activity.

Description

THIENOPYRIMIDINE DERIVATIVES AS PHOSPHODIESTERASE 10 INHIBITORS
[001] This application claims priority to U.S. provisional application no.
60/638,179, filed on December 23, 2004.
[002] Provided are certain thienopyrimi dines, methods of preparing such compounds, compositions containing such compounds, and methods of use thereof. [003] Neurotransmitters and hormones, as well as other types of extracellular signals such as light and odors, create intracellular signals by altering the amounts of cyclic nucleotide monophosphates (cAMP and cGMP) within cells. These intracellular messengers alter the functions of many intracellular proteins. Cyclic AMP regulates the activity of cAMP-dependent protein kinase (PKA). PKA phosphorylates and regulates the function of many types of proteins, including ion channels, enzymes, and transcription factors. Downstream mediators of cGMP signaling also include kinases and ion channels. In addition to actions mediated by kinases, cAMP and cGMP bind directly to some cellular proteins and directly regulate their activity.
[004] Cyclic nucleotides are produced from the actions of adenylyl cyclase and guanylyl cyclase which convert ATP to cAMP and GTP to cGMP, respectively. Extracellular signals, often through the actions of G protein-coupled receptors, regulate the activity of the cyclases. Alternatively, the amount of cAMP and cGMP may be altered by regulating the activity of the enzymes that degrade cyclic nucleotides. Cell homeostasis is maintained by the rapid degradation of cyclic nucleotides after stimulus- induced increases. The enzymes that degrade cyclic nucleotides are called 3',5'-cyclic nucleotide-specific phosphodiesterases (PDEs).
[005] Eleven PDE gene families (PDEl-PDEl 1) have been identified so far, based on their distinct amino acid sequences, catalytic and regulatory characteristics, and sensitivity to small molecule inhibitors. These PDE families are coded for by 21 genes; and further multiple splice variants are transcribed from many of these genes. Expression patterns of each of the gene families are distinct. PDEs differ with respect to their affinity for cAMP and cGMP. Activities of different PDEs are regulated by different signals. For example, PDEl is stimulated by Ca2+/calmodulin, PDE2 activity is stimulated by cGMP, PDE3 is inhibited by cGMP, PDE4 is cAMP specific and is specifically inhibited by rolipram, PDE5 is cGMP-specific and PDE6 is expressed in retina. Less is known about the expression patterns and functional attributes of the higher number PDEs (7 through 11).
[006] PDEl 0 sequences were first identified by using bioinformatics and sequence information from other PDE gene families (Fujishige et al., J. Biol. Chem. 274:18438-18445, 1999; Loughney, K. et al., Gene 234:109-117, 1999; Soderling, S. et al., Proc. Natl. Acad. Sci. USA 96:7071-7076, 1999). PDElO is defined as a unique gene family based on its amino acid sequence, functional properties and tissue distribution. The human PDElO gene is large, over 200 kb, with up to 24 exons coding for each of the splice variants. The amino acid sequence is characterized by two GAF domains (which bind cGMP), a catalytic region, and alternatively spliced N and C termini. Numerous splice variants are possible because of at least 3 alternative exons encoding the N and 2 for the C-termini. PDElOAl, a splice variant of PDElO, is a 779 amino acid protein that hydrolyzes both cAMP and cGMP. The K1n values for cAMP and cGMP are 0.05 and 3.0 micromolar, respectively. In addition to human variants, several variants with high homology have been isolated from both rat and mouse tissues and sequence banks. [007] PDEl 0 transcripts were initially detected in RNA from human testis and brain. Immunohistochemical analysis identified specific brain regions enriched in PDElO. The basal ganglia express the highest amounts of PDElO. Specifically, striatal neurons in the olfactory tubercle, caudate nucleus and nucleus accumbens are enriched in PDElO (Seeger, T. F. et al., Brain Res. 2003, Sept. 26, 985(2): 113-26). Western blots did not reveal the expression of PDElO in other brain tissues, although immunprecipitation of the PDElO complex was possible in hippocampal and cortical tissues. This suggests that the expression level of PDElO in these other tissues is 100-fold less than in striatal neurons. Expression in hippocampus is limited to the cell bodies, whereas PDElO is expressed in terminals, dendrites and axons of striatal neurons.
[008] The tissue distribution of PDElO indicates that PDElO inhibitors may play an important role in the basal ganglia. PDElO selective inhibitors could be used to raise levels of cAMP and/or cGMP within cells that express the PDElO enzyme, especially neurons that comprise the basal ganglia. Selective PDElO inhibition could lead to altered basal ganglia function and may be effective in treating a variety of neuropsychiatric conditions involving the basal ganglia.
[009] Provided is at least one chemical entity chosen from compounds of
Formula I:
Figure imgf000004_0001
(Formula I) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-I0 aryl;
(ii) substituted C6-10 aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, Ci-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Cp 8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Ci-8 alkylsulphinyl, Cp8 alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio; (iii) heterocyclyl; (iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, C1-4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Ci-g alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and (vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C]-S alkyl, halogenated Q-g alkyl, Ci-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, C1-S alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
R2 is chosen from C2-4 hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)nYR5 and - (CR6R7)mYR5 where:
X is chosen from -O-, -S- and -NR9- where R9 is chosen from H, Ci-8 alkyl; and C3-8 cycloalkyl, substituted Cp8 alkyl; and substituted C3-8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo;
Y is chosen from a covalent bond, -O-, -S- and -NR9-; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R3 and R4 are each independently chosen from: 0) H; (ii) halo; (iii) CM2 alkyl; (iv) C2-12 alkenyl; (v) C2-I2 alkynyl; (vi) C3-12 cycloalkyl;
(vii) substituted Cj-I2 alkyl chosen from mono-, di-, and tri-substituted C1-12 alkyl and wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated C1-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Cj ^-alkylamino, Ci ^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci ^-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, C1-4-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, CM-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C^-alkylsulphinyl, and
Figure imgf000006_0001
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Cμ- hydroxyalkyl, C2-4-hydroxyalkoxy,
Figure imgf000006_0002
and C M-alkylsulphonyl; R6 and R7 are independently chosen from
(i) H; (ii) halo; (iii) Ci-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted CM2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, C^-hydroxyalkyl, C2-4- hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Cj-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, C1-4-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-12 alkynylwherein the substituents are independently chosen from halo, hydroxy, C1-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, C1-4 alkylamino,
Figure imgf000007_0001
CM-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, C1-4- hydroxyalkyl, C2-4-hydroxyalkoxy, C1-4-alkylthio, C^-alkylsulphinyl, and d^-alkylsulphonyl; and
R5 is chosen from C6-I0 aryl, substituted C6-J0 aryl, Het, substituted Het, C6-I0 aryl- Cr8 alkyl, substituted C6-I0 aryl-Ci-8 alkyl, Het-Ci-g alkyl, and substituted Het-Ci-8 alkyl, wherein the alkyl portions of C6-I0 aryl-Ci-g alkyl and Het- Ci-g alkyl are optionally substituted by oxo and wherein: substituted C6-I0 aryl is chosen from mono-, di-, and tri-substituted C6-10 aryl wherein the substituents are independently chosen from
(1) Cr8 alkyl,
(2) C3-8 cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-S cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo (such as F or Cl),
(8) amino,
(9) cyano,
(10) hydroxyl, (11) nitro,
(12) Ci-ghalogenated alkyl,
(13) C i -8 halogenated alkoxy,
(14) C i -8 hydroxyalkyl,
(15) C2-8 hydroxyalkoxy,
(16) C3-8 alkenyloxy,
(17) C1 -8 alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-β alkylthio,
(37) Ci -8 alkylsulphinyl,
(38) C i -8 alkylsulphonyl,
(39) Ci-g alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol, (42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) C6-I0 aryl,
(45) substituted C6-J0 aryl chosen from mono-, di-, and trisubstituted C6- io aryl wherein the substituents are independently chosen from Q -8 alkyl, halogenated d-8 alkyl, C 1-4 alkoxy, halogenated Cp4 alkoxy, Cj-g hydroxyalkyl, C2-g hydroxyalkoxy, amino, C1-S alkylamino, di-Ci-g alkylamino, C1-S alkylsulphinyl, Ci-g alkylsulphonyl, Ci-g alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(46) heterocyclyl;
(47) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci-g alkyl, halogenated Ci-g alkyl, C1-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Ci-g alkyl, hydroxy C2-8 alkoxy, amino, Ci-g alkylamino, di-Ci-g alkylamino, Ci-g alkylsulphinyl, Ci-8 alkylsulphonyl, Q-g alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(48) heterocyclyl-carbonyl; and
(49) heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, C 1-4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Q-s alkylamino, C1-8 alkylsulphinyl, Cr8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio; and
Het is chosen from heterocyclyl, substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl, heteroaryl, and substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl, wherein the substituents are independently chosen from
(1) C1-S alkyl,
(2) C3-g cycloalkyl, (3) C4-8 cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(H) nitro,
(12) Ci-8halogenated alkyl,
(13) Cj-shalogenated alkoxy,
(14) Ci-ghydroxyalkyl,
(15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) Ci-8 alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-, (34) NH2NHCONH-,
(35) acyloxy,
(36) C1-S alkylthio,
(37) C,-8 alkylsulphinyl,
(38) Ci-8 alkylsulphonyl,
(39) Ci-8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-20 aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, Cr4 alkoxy, halogenated Cr4 alkoxy, Cr8 hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Crs alkylamino, di-Crs alkylamino, Ci -8 alkylsulphinyl, Cj -8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Crs alkyl, Cr4 alkoxy, halogenated Cj -4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Cr8 alkylamino, Crs alkylsulphinyl, Cr8 alkylsulphonyl, Crs alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, Cr4 alkoxy, halogenated Ci-4 alkoxy, hydroxy C\s alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, Cp8 alkylsulphinyl, C1-S alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cr8 alkyl, halogenated C1-S alkyl, Cr4 alkoxy, halogenated Cr4 alkoxy, hydroxy C1-S alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, C j-g alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cr8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio, and
R9 is chosen from H, Cp8 alkyl, C3-8 cycloalkyl, substituted Cp8 alkyl chosen from mono-, di-, and trisubstituted Cp8 alkyl, and substituted C3-8 cycloalkyl chosen from mono-, di-, and trisubstituted C3-8 cycloalkyl wherein the substituents are independently chosen from halo, Cp4-alkyl, Cp4-alkoxy, and oxo; and provided that the compound of Formula I is not chosen from :
(i) when R1 is 4-methylphenyl, then R2 is not -O-p-phenylene-O- (CH2)phenyl, -O-p-phenylene-NH-C(O)-thiophen-2-yl, -O-(CH2)2-O-(phenyl), - NH(CH2)2-(4-hydroxyphenyl), -O-(CH2)2-(l,3-dioxoisoindolin-l-yl), -NH(CH2)2-(2-oxo- imidaziolidin-1-yl), -NH(CH2)2-(3,4,-dimethoxyphenyl), -S(CH2)2-phenyl, -NH(CH2)2- phenyl, -NH(CH2)2-morpholin-4-yl, -NH(CH2)2-thiophen-2-yl, -NH(CH2)2-(3,4- methylenedioxyphenyl), -NH(CH2)3-morpholin-4-yl, or-S(CH2)3-phenyl;
(ii) 2-methoxy-4-[[[2-[(5-phenylthieno[2,3-d]pyimidin-4- yl)amino]ethyl]imino]-methyl]-phenol;
(iii) 4,4'-[l,3-phenylenebis(oxy)bis[5-(4-chlorophenyl)-thieno[2,3- d]pyrimidine;
(iv) when R1 is phenyl, then R2 is not -O-p-phenylene-NH-C(O)-3,-4- dimethoxyphenyl, -O-(CH2)2-O-(4-methylphenyl), -O-(CH2)2-Ophenyl, -O-(CH2)2-O(3- bromophenyl), -NH-(CH2)2-NH(3-CF3-pyridin-2-yl), -S-(CH2)2-O-(3-methylphenyl), -S- (CH2)2-O-(4-Fphenyl), -S-(CH2)2-O-(2,4-dichlorophenyl), -NH-(CH2)2-NH(4-CF3- ρyrimidin-2-yl), -NH-(CH2)2-O-(3-acetylaminophenyl), -S-(CH2)3-phenyl, -S-(CH2)4- (1,3-dioxoisoindolin-l-yl), -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)3-imidazol-l-yl, -NH- (CH2)3-(2-oxo-pyrrolidin-l-yl), -N(CH3)-(CH2)2-pyridin-2-yl, -NH-(CH2)2-pyridin-2-yl, - S-(CH2)2-(l,3-dioxoisoindolin-l-yl, -S-(CH2)2-phenyl, -NH-(CH2)2-(2-chlorophenyl), - NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-(3 ,4-dimethoxyphenyl), -NH-(CH2)2-thiophen- 2-yl, or-NH-(CH2)2-phenyl;
(v) when R1 is 4-fluorophenyl, then R2 is not chosen from -O-(CH2)2-O-(2- chlorophenyl), -O-(CH2)2-O-(4-fluorophenyl), -S-(CH2)3-phenyl, -NH-(CH2)3-phenyl, - NH-(CH2)2-phenyl, -NH-(CH2)3-moφholin-4-yl, -NH-(CH2)3-(2-oxo-pyrrolidin-l-yl), - NH-(CH2)2-morpholin-4-yl, -S-(CH2)2-ρhenyl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2- moφholin-4-yl, -NH-(CH2)2-(3 ,4-methylenedioxyphenyl), -NH-(CH2)2-thiophen-2-yl, and -NH-(CH2)2-(3 ,4-ethylenedioxyphenyl);
(vi) when R1 is 4-chlorophenyl, then R2 is not chosen from -O-p-phenylene-O- CH2-phenyl, -O-(CH2)2-O-(4-methylphenyl), -S-(CH2)4-(l,3-dioxoisoindolin-l-yl), -S- (CH2)3-phenyl, -S-(CH2)2-phenyl, -NH-(CH2)3-imidazol-l-yl, -NH-(CH2)3-morpholin-4- yl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2-(1,3- dioxoisoindolin-1-yl), -NH-(CH2)2-(3 ,4-methylenedioxyphenyl), -NH-(CH2)2-phenyl, - NH-(CH2)2-thiophen-2-yl, -NH-(CH2)2-(3 ,4-ethylenedioxyphenyl), -S-(CH2)4-(1 ,3- dioxoisoindolin-1-yl), -NH(CH2)3-(2-oxo-pyrrolidin-l-yl), and -NH(CH2)2-(3,4,- dimethoxyphenyl);
(vii) when R1 is 3-methylphenyl, then R2 is not -O-(CH2)2-O-(phenyl);
(viii) when R1 is 3-thiophen-2-yl, then R2 is not chosen from -NH-(CH2)2- morpholin-4-yl, -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)2-phenyl, and -NH-(CH2)2-(3 ,4- dimethoxyphenyl); (ix) when R1 is 4-methoxyphenyl, then R2 is not chosen from -S-(CH2)3- phenyl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-C(CH3)2-morpholin-4-yl, - NHCH2C(CH3)2-moφholin-4-yl, -N(CH3)-(CH2)2-(3 ,4-dimethoxyphenyl), -S-(CH2)2- phenyl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(x) when R1 is 3,4-dimethylphenyl, then R2 is not chosen from -O-p- phenylene-O-CH2-phenyl, -S-(CH2)3-phenyl, -S-(CH2)3-morpholin-4-yl, -S-(CH2)2- phenyl, -NH-(CH2)3-(4-hydroxyphenyl), -0-(CH2)2-(l,3-dioxoisoindolin-l-yl), -NH- (CH2)2- moφholin-4-yl, -NH-(CH2)3- morpholin-4-yl, -NH-(CH2)2-(3,4- methylenedioxyphenyl), -S-(CH2)2-phenyl, -NH-(CH2)2-phenyl, -NH-(CH2)2-thiophen-2- yl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(xi) when R1 is 4-bromophenyl, then R2 is not chosen from -(CH2)3-phenyl, -S- (CH2)3-moφholin-4-yl, -S-(CH2)2-phenyl, -S-(CH2)3-ρhenyl, -NH-(CH2)3-moφholin-4- yl, -NH-(CH2)3-(2-oxo-pyrrolidin-l-yl), and -NH-(CH2)2-(3,4-ethylenedioxyphenyl); and
(xii) when R1 is 4-biphenyl, then R2 is not -NH-(CH2)2-(3,4- ethylenedioxyphenyl);
(xiii) when R1 is 2,3-dihydro-l ,4-benzodioxin-2-yl, then R2 is not NH(CH2)2- phenyl, and
(xiv) 5-(2-chlorophenyl)-4-[4-(phenylmethoxy)phenoxy]-thieno[2,3- d]pyrimidine.
[010] Provided is at least one chemical entity chosen from compounds of
Formula I
Figure imgf000014_0001
(Formula I) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-I0 aryl; (ii) substituted C6-io aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, Ci-4alkoxy, halogenated Cj-4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(iii) heterocyclyl;
(iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, Ci-4 alkoxy, halogenated Q-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-g alkylamino, di-Ci-g alkylamino, Ci-8 alkylsulphinyl, Cp8 alkylsulphonyl, Cj-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and
(vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C i-8 alkyl, halogenated Ci -8 alkyl, Q-4 alkoxy, halogenated Cp4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Q-s alkylsulphonyl, Q-s alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
R2 is chosen from C2-4 hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)πYR5 and - (CR6R7)mYR5 where:
X is chosen from -0-, -S- and -NR9-;
Y is chosen from a covalent bond, -0-, -S- and -NR9-; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R3 and R4 are each independently chosen from: (i) H; (ii) halo; (iii) CM2 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted CM2 alkyl chosen from mono-, di-, and tri-substituted Ci-I2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Cj-4 alkylamino, di-C^-alkylamino, Ci^-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Q-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, CM-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Q ^-alkylamino, CM-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Q-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Q ^-alkylamino, Q-4- hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Q-4-alkylsulphinyl, and Ci^-alkylsulphonyl; and
(x) substituted C3-J2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Q-4- hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and C i ^-alkylsulphonyl ;
R6 and R7 are independently chosen from 0) H; (ii) halo; (iii) C1-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted Cj-I2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, CM-hydroxyalkyl, C2-4- hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, CM-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynylwherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, CM-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and C i .4-alkylsulphonyl;
R9 is chosen from H, Ci-g alkyl, C3-g cycloalkyl, substituted Ci-8 alkyl chosen from mono-, di-, and trisubstituted Q-g alkyl, and substituted C3-« cycloalkyl chosen from mono-, di-, and trisubstituted C3-8 cycloalkyl wherein the substituents are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo; and
R5 is chosen from pyridin-3-yl and pyridin-4-yl, each of which is optionally substituted with one or two groups chosen from
(1) d-s alkyl,
(2) C3-8 cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) d-β alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro,
(12) Ci-g halogenated alkyl,
(13) Ci-8halogenated alkoxy,
(14) d-shydroxyalkyl,
(15) C2-8 hydroxyalkoxy
( 16) C3-8 alkenyloxy,
(17) C i-8 alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea, (28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-8 alkylthio,
(37) d-8 alkylsulphinyl,
(38) Cr8 alkylsulphonyl,
(39) C1-S alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-M aiyl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-2o aryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, C1-4 alkoxy, halogenated C)-4 alkoxy, Ci-8 hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, C i-8 alkylsulphinyl, C1-S alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Ci-s alkyl, halogenated Ci-8 alkyl, Ci-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy d-8 alkyl, hydroxy C2-8 alkoxy, amino, Q-8 alkylamino, di-Cr8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Ci-8 alkyl, Ci-4 alkoxy, halogenated Q-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Cr8 alkylamino, Cp8 alkylsulphinyl, Ci -8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, C1-S alkyl, halogenated Cj-8 alkyl, Cr4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Cpg alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, C1-S alkylsulphinyl, Cr8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated C1-S alkyl, Cr4 alkoxy, halogenated Cr4 alkoxy, hydroxy Crs alkyl, hydroxy C2-8 alkoxy, amino, Crs alkylamino, di-Ci-8 alkylamino, C1-S alkylsulphinyl, C1-S alkylsulphonyl, C,-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
[Oi l] Provided is at least one chemical entity chosen from compounds of
Formula I:
Figure imgf000021_0001
(Formula I) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-I0 aryl;
(ii) substituted C6- 10 aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy C1- 8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, C]-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(iii) heterocyclyl;
(iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cps alkylamino, C1-S alkylsulphinyl, d-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and
(vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, Ci-s alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio; R is chosen from C2-4 hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)nYR5 and - (CR6R7)mYR5 where:
X is chosen from -O-, -S- and -NR9- where R9 is chosen from H, C1-S alkyl; and C3-8 cycloalkyl, substituted Cj-g alkyl; and substituted C3-8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo;
Y is chosen from a covalent bond, -O-, -S- and -NR9-; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R and R4 are each independently chosen from: (i) H; (ii) halo; (iii) C1-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-J2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted C1-I2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, C1-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci ^-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, CM-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci ^-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, C1-4-alkylsulphinyl, and Q-4- alkyl sulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Cj-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, d^-alkylthio, C^-alkylsulphinyl, and Ci-4-alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, d^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy,
Figure imgf000023_0001
Ci-4-alkylsulphinyl, and C i ^-alkylsulphonyl ;
R and R are independently chosen from (i) H; (ii) halo; (iii) Ci-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-J2 cycloalkyl;
(vii) substituted CM2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino,
Figure imgf000023_0002
C2-4- hydroxyalkoxy, C^-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, C^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C^-alkylsulphinyl, and Cμ- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynylwherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated C1-4 alkoxy, nitro, cyano, carboxy, amino, C1-4 alkylamino, di-Ci-4-alkylamino, C^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, CM-alkylsulphinyl, and Ci-4- alkylsulphonyl; and
(x) substituted C3-J2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, C1-4 alkylamino, di-CM-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, d-4-alkylsulphinyl, and C i -4-alkylsulphonyl ;
R9 is chosen from H, Cr8 alkyl, C3-8 cycloalkyl, substituted Cp8 alkyl chosen from mono-, di-, and trisubstituted C)-g alkyl, and substituted C3-8 cycloalkyl chosen from mono-, di-, and trisubstituted C3-8 cycloalkyl wherein the substituents are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo; and
R5 is chosen from pyridin-2-yl and pyridin-4-yl, each of which is substituted with one or two groups chosen from
(1) C-8 alkyl,
(2) C3-S cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-S cycloalkoxy,
(6) C4-g cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro,
(12) C2-S halogenated alkyl,
(13) Cr8 halogenated alkoxy,
(14) d-s hydroxyalkyl, (15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) Cp8 alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-g alkylthio,
(37) C,-8 alkylsulphinyl,
(38) Ci-8 alkylsulρhonyl,
(39) C1-S alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-M aryl, (46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Ci-g alkyl, C1-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-g alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cr8 alkylsulphonyl, Cj-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Cp8 alkyl, C 1-4 alkoxy, halogenated Cp4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cr8 alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, C^8 alkyl, halogenated C 1-8 alkyl, Cp4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated C1-S alkyl, C1-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and (54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cr8 alkyl, C]-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
[012] Provided is at least one chemical entity chosen from compounds of Formula II:
Figure imgf000027_0001
(Formula II) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-Io aryl;
(ii) substituted C6-10 aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, C 1-4 alkoxy, halogenated C1 -4 alkoxy, hydroxy Cj- 8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, C 1-8 alkylsulphonyl, d-8 alkylsulphonamido, -COR , -CSR9, -SR9, cyano, hydroxyl, nitro, and thio; (iii) heterocyclyl; (iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, Cp8 alkyl, halogenated Crs alkyl, C1-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-g alkoxy, amino, Ci-8 alkylamino, di-Cr8 alkylamino, Ci-8 alkylsulphinyl, C(-8 alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and
(vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C 1-8 alkyl, halogenated Cj-8 alkyl, C 1-4 alkoxy, halogenated Cp4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-g alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Ci-8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
R2 is chosen from C2-4 hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)nYR5 and - (CR6R7)mYR5 where:
X is chosen from -O-, -S- and -NR9- where R9 is chosen from H, Ci-8 alkyl; and C3-8 cycloalkyl, substituted Cp8 alkyl; and substituted C3-8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo;
Y is chosen from a covalent bond, -O-, -S- and -NR -; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R3 and R4 are each independently chosen from: (i) H; (ii) halo; (iii) CM2 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted C1-I2 alkyl chosen from mono-, di-, and tri-substituted Ci-I2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, CM-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, C^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Cj-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, C^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C^-alkylsulphinyl, and Q-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, CM alkylamino, di-Ci ^-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy,
Figure imgf000029_0001
and Ci-4-alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Ci-4-alkylsulphonyl;
R6 and R7 are independently chosen from (i) H; (ii) halo; (iii) Ci2 alkyl; (iv) C2-I2 alkenyl; (v) C2-J2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted Ci-I2 alkyl chosen from mono-, di-, and tri-substituted Ci-I2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated C1-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, Ci-4-hydroxyalkyl, C2-4- hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, CM-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynylwherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci^-hydroxyalkyl, C2-4-hydroxyalkoxy, d^-alkylthio, CM-alkylsulphinyl, and Ci-4- alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, Ci-4- hydroxyalkyl,
Figure imgf000030_0001
and C 1 -4-alkylsulphonyl;
R9 is chosen from H, Ci-8 alkyl, C3-8 cycloalkyl, substituted Cp8 alkyl chosen from mono-, di-, and trisubstituted Cj-8 alkyl, and substituted C3-8 cycloalkyl chosen from mono-, di-, and trisubstituted C3-8 cycloalkyl wherein the substituents are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo; p is chosen from 1, 2, 3, and 4; and for each occurrence, R10 is independently chosen from
(1) Cr8 alkyl,
(2) C3-8 cycloalkyl, (3) C4-8 cycloalkylalkyl,
(4) C3-s cycloalkoxy,
(5) C4-8 cycloalkylalkoxy,
(6) amino,
(7) cyano,
(8) nitro,
(9) Ci-ghalogenated alkyl,
(10) C1-S halogenated alkoxy,
(H) C i-8 hydroxyalkyl,
(12) C2-S hydroxyalkoxy,
(13) Ci-S alkylamino,
(14) di-C i -salkylamino,
(15) carboxy,
(16) alkoxycarbonyl,
(17) aminocarbonyl,
(18) hydroxyaminocarbonyl,
(19) alkylaminocarbonyl,
(20) dialkylaminocarbonyl,
(21) urea,
(22) hydroxyurea,
(23) alkylurea,
(24) dialkylaminoalkylcarbonyl,
(25) aminocarbonylalkylaminocarbonyl,
(26) acylamido,
(27) HCO-NH-NH-CO-,
(28) NH2NHCO-,
(29) NH2NHCONH-,
(30) acyloxy,
(31) Ci-s alkylthio,
(32) Ci-S alkylsulphinyl,
(33) Ci-s alkylsulphonyl, (34) Ci-8 alkylsulphonamido,
(35) (alkylsuphonyl)2amino-,
(36) thiol,
(37) aminosulfonyl,
(38) alkylaminosulfonyl,
(39) C6-20 aryl,
(40) substituted C6-2o aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, C i-g alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated C 1-4 alkoxy, Ci-8 hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Cr8 alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(41) heterocyclyl-carbonyl,
(42) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, Cr4 alkoxy, halogenated C1-4 alkoxy, hydroxy Cj-8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(43) heteroaryl-carbonyl, and
(44) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cp8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
[013] Also provided is a pharmaceutical composition comprising at least one chemical entity described herein and a pharmaceutically acceptable carrier.
[014] Also provided is a method of selectively inhibiting PDElO enzyme in a patient in need thereof comprising administering to said patient an effective amount of at least one chemical entity described herein.
[015] Unless otherwise specified the following terms in the specification and the claims in this Application have the meanings given below. [016] Halo herein refers to F, Cl, Br, and I, such as F and Cl.
[017] Alkyl means a straight-chain or branched-chain aliphatic hydrocarbon radical. Suitable alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, and dodecyl. Other examples of suitable alkyl groups include 1-, 2- or 3-methylbutyl, 1,1-, 1,2- or 2,2-dimethylpropyl, 1-ethylpropyl, 1-, 2-, 3- or 4-methylpentyl, 1,1-, 1,2-, 1,3-, 2,2-, 2,3- or 3,3-dimethylbutyl, 1- or 2-ethylbutyl, ethylmethylpropyl, trimethylpropyl, methylhexyl, dimethylpentyl, ethylpentyl, ethylmethylbutyl, dimethylbutyl, and the like.
[018] Alkenyl refers to an unsaturated branched or straight-chain alkyl group having at least one carbon-carbon double bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkene. The group may be in either the cis or trans configuration about the double bond(s). Typical alkenyl groups include, but are not limited to, ethenyl; propenyls such as prop-1-en-l-yl, prop-l-en-2-yl, prop-2-en-l-yl (allyl), prop-2-en-2-yl, cycloprop-1-en-l-yl; cycloprop-2-en-l-yl; butenyls such as but-1-en-l-yl, but-l-en-2-yl, 2-methyl-prop-l-en-l-yl, but-2-en-l-yl, but-2-en-l-yl, but-2-en-2-yl, buta-l,3-dien-l-yl, buta-l,3-dien-2-yl, cyclobut-1-en-l-yl, cyclobut-l-en-3-yl, cyclobuta-l,3-dien-l-yl; and the like. In certain embodiments, an alkenyl group has from 2 to 20 carbon atoms and in other embodiments, from 2 to 6 carbon atoms. Alkenylene refers to a divalent alkenyl group.
[019] Alkynyl refers to an unsaturated branched or straight-chain alkyl group having at least one carbon-carbon triple bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkyne. Typical alkynyl groups include, but are not limited to, ethynyl; propynyls such as prop-1-yn-l-yl, prop-2-yn-l-yl; butynyls such as but-1-yn-l-yl, but-l-yn-3-yl, but-3-yn-l-yl; and the like. In certain embodiments, an alkynyl group has from 2 to 20 carbon atoms and in other embodiments, from 3 to 6 carbon atoms. Alkynylene refers to a divalent alkynyl group. [020] Cycloalkyl herein refers to a non-aromatic monocyclic, bicyclic, or tricyclic carbocyclic ring, usually having from 3 to 8 ring carbon atoms, for example 4 to 6 ring carbon atoms. The ring may be saturated or have one or more carbon-carbon double bonds. Examples of cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl, as well as bridged and caged saturated ring groups such as norbornane. Other suitable cycloalkyl groups include spiropentyl, bicyclo[2.1.0]pentyl, bicyclo[3.1.0]hexyl, spiro[2.4]heptyl, spiro[2.5]octyl, bicyclo[5.1.0]octyl, spiro[2.6]nonyl, bicyclo[2.2.0]hexyl, spiro[3.3]heptyl, and bicyclo[4.2.0]octyl. [021] In the arylalkyl groups and heteroarylalkyl groups, "alkyl" refers to a divalent alkylene group having, in certain embodiments, 1 to 4 carbon atoms. [022] In the cases where alkyl is a substituent (e.g., alkyl substituents on aryl and heteroaryl groups) or is part of a substituent (e.g., in the alkylamino, dialkylamino, hydroxyalkyl, hydroxyalkoxy, alkylthio, alkylsulphinyl, and alkylsulphonyl substituents), the alkyl portion usually has 1 to 12 carbon atoms, for example 1 to 8 carbon atoms, such as 1 to 4 carbon atoms.
[023] Aryl, as a group or substituent per se or as part of a group or substituent, refers to an aromatic, monocyclic or bicyclic carbocyclic radical containing 6 to 14 carbon atoms, such as 6 to 12 carbon atoms, for example 6 to 10 carbon atoms. Suitable aryl groups include phenyl, naphthyl and biphenyl. Unless otherwise specified, substituted aryl groups include the above-described aryl groups which are substituted by at least one time, for example, one, two, or three times, by a group chosen from halo, alkyl, hydroxy, alkoxy, nitro, methylenedioxy, ethylenedioxy, amino, alkylamino, dialkylamino, hydroxyalkyl, hydroxyalkoxy, carboxy, cyano, acyl, alkoxycarbonyl, alkylthio, alkylsulphinyl, alkylsulphonyl, phenoxy, and acyloxy (e.g., acetoxy). [024] Arylalkyl refers to an aryl-alkyl-radical in which the aryl and alkyl portions are in accordance with the previous descriptions. Suitable examples include 1- phenethyl, 2-phenethyl, phenpropyl, phenbutyl, phenpentyl, and naphthylenemethyl. [025] Heteroaryl encompasses: 5- to 7-membered aromatic, monocyclic rings containing one or more, for example, from 1 to 4, or in certain embodiments, from 1 to 3, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon; bicyclic heterocyclyl rings containing one or more, for example, from 1 to 4, or in certain embodiments, from 1 to 3, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring; and tricyclic heterocyclyl rings containing one or more, for example, from 1 to 5, or in certain embodiments, from 1 to 4, such as 1 or 2, heteroatoms chosen from N, O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring.
[026] For example, heteroaryl includes a 5- to 7-membered heterocyclyl, aromatic ring fused to a 5- to 7-membered cycloalkyl or heterocyclyl ring. For such fused, bicyclic heteroaryl ring systems wherein only one of the rings contains one or more heteroatoms, the point of attachment may be at either ring. When the total number of S and O atoms in the heteroaryl group exceeds 1, those heteroatoms are not adjacent to one another. In certain embodiments, the total number of S and O atoms in the heteroaryl group is not more than 2. In certain embodiments, the total number of S and O atoms in the aromatic heterocycle is not more than 1. Examples of heteroaryl groups include, but are not limited to, furyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, pyridyl, pyrimidinyl, indolyl, quinolinyl, naphthyridinyl, azaindolyl (e.g.,7-azaindolyl), 1,2,3,4,- tetrahydroisoquinolyl, and the like. In certain embodiments, examples of heteroaryl groups include, but are not limited to 2-thienyl, 3 -thienyl, 2-, 3- or 4-pyridyl, 2-, 3-, 4-, 5- , 6-, 7- or 8-quinolinyl, 7- azaindolyl, and 1-, 3-, 4-, 5-, 6-, 7- or 8-isoquinolinyl. Unless otherwise specified, substituted heteroaryl groups refer to the heteroaryl groups described above which are substituted in one or more places by halo, aryl, alkyl, alkoxy, cyano, halogenated alkyl (e.g., trifluoromethyl), nitro, oxo, amino, alkylamino, and dialkylamino. Heteroaryl does not encompass or overlap with aryl, cycloalkyl, or heterocyclyl, as defined herein
[027] Substituted heteroaryl also includes ring systems substituted with one or more oxide (-0') substituents, such as pyridinyl N-oxides. [028] Heteroarylalkyl refers to a heteroaryl-alkyl-group wherein the heteroaryl and alkyl portions are in accordance with the previous discussions. Suitable examples are pyridylmethyl, thienylmethyl, pyrimidinylmethyl, pyrazinylmethyl, isoquinolinylmethyl, pyridylethyl and thienylethyl.
[029] By heterocyclo alkyl or heterocyclyl is meant a single, non-aromatic ring, usually with 3 to 7 ring atoms, containing at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms. Heterocyclyl also includes bicyclic ring systems wherein one non-aromatic ring, usually with 3 to 7 ring atoms, contains at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms; and the other ring, usually with 3 to 7 ring atoms, optionally contains 1-3 heteratoms independently selected from oxygen, sulfur, and nitrogen and is not aromatic. The ring or rings may be saturated or have one or more carbon-carbon double bonds. Suitable heterocyclyl groups include, for example (as numbered from the linkage position assigned priority 1), 3-tetrahydrofuranyl, piperidinyl, imidazolinyl, imidazolidinyl, pyrrolinyl, pyrrolidinyl, morpholinyl, piperazinyl, oxazolidinyl, and indolinyl. Substituted heterocyclyl also includes ring systems substituted with one or more oxo (=0) or oxide (-O") substituents, such as piperidinyl N- oxide, morpholinyl -N-oxide, 1-oxo-l-thiomorpholinyl and 1,1-dioxo-l-thiomorpholinyl. [030] Acyl refers to alkanoyl radicals having 2 to 4 carbon atoms, such as formyl, acetyl, propionyl, and butanoyl.
[031] Substituted radicals usually have 1 to 3 substituents, for example, 1 or 2 substituent, such as 1 substituent.
[032] Compounds of Formula I include, but are not limited to, optical isomers of compounds of Formula I, racemates, and other mixtures thereof. In those situations, the single enantiomers or diastereomers, i.e., optically active forms, can be obtained by asymmetric synthesis or by resolution of the racemates. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral high- pressure liquid chromatography (HPLC) column. Substantially pure enantiomers contain no more than 5% w/w of the corresponding opposite enantiomer, such as no more than 2%, for example, no more than 1%. In addition, compounds of Formula I include Z- and E- forms (or cis- and trans- forms) of compounds with carbon-carbon double bonds. Where compounds of Formula I exists in various tautomeric forms, chemical entities of the present invention include all tautomeric forms of the compound. [033] Chemical entities of the present invention include compounds of Formula
I and all pharmaceutically acceptable forms thereof. Pharmaceutically acceptable forms of the compounds recited herein include pharmaceutically acceptable salts, solvates, crystal forms (including polymorphs and clathrates), chelates, non-covalent complexes, prodrugs, and mixtures thereof. In certain embodiments, the compounds described herein are in the form of pharmaceutically acceptable salts. Hence, the terms "chemical entity" and "chemical entities" also encompass pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures.
[034] A polymorph is a solid crystalline phase of a compound with at least two different arrangements or polymorphic forms of that compound molecule in the solid state. Polymorphic forms of any given compound are defined by the same chemical formula or composition and are as distinct in chemical structure as crystalline structures of two different chemical compounds.
[035] Pharmaceutically acceptable salts include those obtained by reacting the main compound, functioning as a base, with an inorganic or organic acid to form a salt, for example, salts of hydrochloric acid, sulfuric acid, phosphoric acid, methanesulfonic acid, camphorsulfonic acid, oxalic acid, maleic acid, succinic acid and citric acid. Other examples of acid salts that can be obtained by reaction with inorganic or organic acids: acetates, adipates, alginates, citrates, aspartates, benzoates, benzenesulfonates, bisulfates, butyrates, camphorates, digluconates, cyclopentanepropionates, dodecylsulfates, ethanesulfonates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, fumarates, hydrobromides, hydroiodides, 2-hydroxy-ethanesulfonates, lactates, maleates, methanesulfonates, nicotinates, 2-naphthalenesulfonates, oxalates, palmoates, pectinates, persulfates, 3-phenylpropionates, picrates, pivalates, propionates, succinates, tartrates, thiocyanates, tosylates, mesylates and undecanoates. [036] Pharmaceutically acceptable salts also include those in which the main compound functions as an acid and is reacted with an appropriate base to form, e.g., sodium, potassium, calcium, magnesium, ammonium, and choline salts.
[037] Those skilled in the art will recognize various synthetic methodologies that may be used to prepare non-toxic pharmaceutically acceptable addition salts.
[038] As noted above, prodrugs also fall within the scope of chemical entities, for example ester or amide derivatives of the compounds of Formula I. The term
"prodrugs" includes any compounds that become compounds of Formula I when administered to a patient, e.g., upon metabolic processing of the prodrug. Examples of prodrugs include, but are not limited to, acetate, formate, and benzoate and like derivatives of functional groups (such as alcohol or amine groups) in the compounds of
Formula I.
[039] The term solvate refers to the chemical entity formed by the interaction of a solvent and a compound. Solvates may also form when solvent molecules are incorporated into the crystalline lattice structure of the compound molecule during the crystallization process. Suitable solvates are pharmaceutically acceptable solvates, such as hydrates, e.g., monohydrates, dihydrates, sesquihydrates, and hemihydrates.
[040] One of ordinary skill in the art will further recognize that compounds of
Formulas I and Ia can exist in different solvate forms.
[041 ] The term chelate refers to the chemical entity formed by the coordination of a compound to a metal ion at two (or more) points.
[042] The term non-covalent complex refers to the chemical entity formed by the interaction of a compound and another molecule wherein a covalent bond is not formed between the compound and the molecule. For example, complexation can occur through van der Waals interactions, hydrogen bonding, and electrostatic interactions (also called ionic bonding).
[043] Provided is at least one chemical entity chosen from compounds of
Formula I:
Figure imgf000039_0001
(Formula I) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-Io aryl;
(ii) substituted C6-I0 aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, C]-8 alkyl, halogenated Cp8 alkyl, Ci-4alkoxy, halogenated C 1-4 alkoxy, hydroxy Ci- 8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, C1-S alkylsulphinyl, Ci-8 alkylsulphonyl, d-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(iii) heterocyclyl;
(iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, C 1-8 alkyl, halogenated Cr8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, Cr8 alkylsulphinyl, Cr8 alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and
(vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, C1-8 alkyl, halogenated d-8 alkyl, Cr4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Crs alkylamino, di-Crs alkylamino, Cr8 alkylsulphinyl, C1-S alkylsulphonyl, Crs alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio; R2 is chosen from C2-4 hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)nYR5 and - (CR6R7)mYR5 where:
X is chosen from -O-, -S- and -NR9-;
Y is chosen from a covalent bond, -O-, -S- and -NR9-; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R3 and R4 are each independently chosen from:
(i) H; (ii) halo; (iii) C1-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-12 cycloalkyl;
(vii) substituted Ci-12 alkyl chosen from mono-, di-, and tri-substituted Ci-I2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, Q ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, Ci ^-hydroxyalkyl, C2-4-hydroxyalkoxy,
Figure imgf000040_0001
and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, Ci ^-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, C^-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-J2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio,
Figure imgf000040_0002
and Ci-4-alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, C^-alkylsulphinyl, and C 14-alkylsulphonyl; R6 and R7 are independently chosen from
(i) H; (ii) halo; (iii) Ci-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3- 12 cycloalkyl;
(vii) substituted CM2 alkyl chosen from mono-, di-, and tri-substituted Ci-I2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, CM-hydroxyalkyl, C2-4- hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, CM alkylamino, di-Ci^-alkylamino, CM-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C^-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynylwherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci^-alkylamino, Ci-4-hydroxyalkyl, C2-4-hydroxyalkoxy, C]-4-alkylthio,
Figure imgf000041_0001
and Ci-4- alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci-4-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy,
Figure imgf000042_0001
C1-4-alkylsulphinyl, and C1-4-alkylsulphonyl; and
R5 is chosen from C6-I0 aryl, substituted C6-I0 aryl, Het, substituted Het, C6-I0 aryl- C1-8 alkyl, substituted C6-I0 aryl-Ci-g alkyl, Het-Ci-8 alkyl, and substituted Het-Q-s alkyl, wherein the alkyl portions of C6-I0 aryl-Cj-s alkyl and Het- C 1-8 alkyl are optionally substituted by oxo and wherein: substituted C6-I0 aryl is chosen from mono-, di-, and tri-substituted C6-I0 aryl wherein the substituents are independently chosen from
(1) C1-S alkyl,
(2) C3-8 cycloalkyl,
(3) C4-S cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo (such as F or Cl),
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro,
(12) C 1 -8 halogenated alkyl,
(13) C 1 -8 halogenated alkoxy,
(14) C1-S hydroxyalkyl,
(15) C2-8 hydroxyalkoxy,
(16) C3-S alkenyloxy,
(17) C 1-8 alkylamino,
(18) di-C i-8 alkylamino, (19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl ,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-g alkylthio,
(37) Cr8 alkylsulphinyl,
(38) Cp8 alkylsulphonyl,
(39) Cr8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) C6-I0 aryl,
(45) substituted C6-I0 aryl chosen from mono-, di-, and trisubstituted C6- io aryl wherein the substituents are independently chosen from Ci-8 alkyl, halogenated Cp8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, C 1-8 hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Ci-g alkylamino, di-Ci-g alkylamino, Cj-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(46) heterocyclyl;
(47) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substitutents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, d-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-S alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Ci-g alkyl sulphonyl, Ci-g alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(48) heterocyclyl-carbonyl; and
(49) heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, Cr4 alkoxy, halogenated C1- 4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-g alkylamino, Cj-8 alkylsulphinyl, C1-S alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio; and
Het is chosen from heterocyclyl, substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl, heteroaryl, and substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl, wherein the substituents are independently chosen from
(1) C1-S alkyl,
(2) C3-8 cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) C1-S alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl, (11) nitro,
(12) Cp8 halogenated alkyl,
(13) C1-S halogenated alkoxy,
(14) Ci-s hydroxyalkyl,
(15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) d-8 alkylamino,
(18) di-Cr8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-8 alkylthio,
(37) Ci-s alkylsulphinyl,
(38) Ci-g alkylsulphonyl,
(39) Ci-8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol, (42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-20 aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated C1-S alkyl, Cr4 alkoxy, halogenated Ci-4 alkoxy, Ci-g hydroxyalkyl, C2-8 hydroxyalkoxy, amino, C1-S alkylamino, di-Ci-8 alkylamino, d-8 alkylsulphinyl, Cj-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Crg alkyl, halogenated Cr8 alkyl, C 1-4 alkoxy, halogenated Cr4 alkoxy, hydroxy d-8 alkyl, hydroxy C2-8 alkoxy, amino, Cj-8 alkylamino, di-Crs alkylamino, C1-S alkylsulphinyl, C1-S alkylsulphonyl, d-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, C 1-8 alkyl, halogenated Cr8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Ci -8 alkylamino, di-Cr8 alkylamino, Cr8 alkylsulphinyl, Ci -8 alkylsulphonyl, d-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, C1-4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, (Ii-C1-S alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Q-g alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cr8 alkyl, halogenated Cp8 alkyl, C1-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cj-8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Q-salkylamino, C i-8 alkylsulphinyl, Cp8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio, and
R9 is chosen from H, Ci-g alkyl, C3-8 cycloalkyl, substituted Cr8 alkyl chosen from mono-, di-, and trisubstituted Cp8 alkyl, and substituted C3-8 cycloalkyl chosen from mono-, di-, and trisubstituted C3-S cycloalkyl wherein the substituents are independently chosen from halo, Q-4-alkyl, Q-4-alkoxy, and oxo; and provided that the compound of Formula I is not chosen from :
(i) when R1 is 4-methylphenyl, then R2 is not -O-p-phenylene-0- (CH2)phenyl, -O-p-phenylene-NH-C(O)-thiophen-2-yl, -O-(CH2)2-O-(phenyl), - NH(CH2)2-(4-hydroxyphenyl), -O-(CH2)2-(1 ,3-dioxoisoindolin-l -yl), -NH(CH2)2-(2-oxo- imidaziolidin-1-yl), -NH(CH2)2-(3,4,-dimethoxyphenyl), -S(CH2)2-ρhenyl, -NH(CH2)2- phenyl, -NH(CH2)2-morpholin-4-yl, -NH(CH2)2-thiophen-2-yl, -NH(CH2)2-(3,4- methylenedioxyphenyl), -NH(CH2)3-moφholin-4-yl, or-S(CH2)3-phenyl;
(ii) 2-methoxy-4-[[[2-[(5-phenylmieno[2,3-d]pyimidin-4- yl)amino] ethyl] imino] -methyl] -phenol ;
(iii) 4,4'-[l,3-phenylenebis(oxy)bis[5-(4-chlorophenyl)-thieno[2,3- d]pyrimidine;
(iv) when R1 is phenyl, then R2 is not -O-p-phenylene-NH-C(O)-3,-4- dimethoxyphenyl, -O-(CH2)2-O-(4-methylphenyl), -O-(CH2)2-Ophenyl, -O-(CH2)2-O(3- bromophenyl), -NH-(CH2)2-NH(3-CF3-pyridin-2-yl), -S-(CH2)2-O-(3-methylρhenyl), -S- (CH2)2-O-(4-Fphenyl), -S-(CH2)2-O-(2,4-dichlorophenyl), -NH-(CH2)2-NH(4-CF3- pyrimidin-2-yl), -NH-(CH2)2-O-(3-acetylaminophenyl), - S -(CH2)3 -phenyl, -S-(CH2)4- (1,3-dioxoisoindolin-l-yl), -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)3-imidazol-l-yl, -NH- (CH2)3-(2-oxo-pyrrolidin-l-yl), -N(CH3)-(CH2)2-pyridin-2-yl, -NH-(CH2)2-pyridin-2-yl, - S-(CH2)2-(l,3-dioxoisoindolin-l-yl, -S-(CH2)2-ρhenyl, -NH-(CH2)2-(2-chlorophenyl), - NH-(CH2)2-moφholin-4-yl, -NH-(CH2)2-(3,4-dimethoxyphenyl), -NH-(CH2)2-thiophen- 2-yl, or-NH-(CH2)2-phenyl;
(v) when R1 is 4-fluorophenyl, then R2 is not chosen from -O-(CH2)2-O-(2- chlorophenyl), -O-(CH2)2-O-(4-fluorophenyl), -S-(CH2)3-ρhenyl, -NH-(CH2)3-phenyl, - NH-(CH2)2-phenyl, -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)3-(2-oxo-pyrrolidin-l-yl), - NH-(CH2)2-moφholin-4-yl, -S-(CH2)2-phenyl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2- morpholin-4-yl, -NH-(CH2)2-(3 ,4-methylenedioxyphenyl), -NH-(CH2)2-thiophen-2-yl, and -NH-(CH2)2-(3,4-ethylenedioxyphenyl);
(vi) when R1 is 4-chlorophenyl, then R2 is not chosen from -O-p-phenylene-O- CH2-phenyl, -O-(CH2)2-O-(4-methylphenyl), -S-(CH2)4-( 1,3-dioxoisoindolin-l-yl), -S- (CH2)3-phenyl, -S-(CH2)2-phenyl, -NH-(CH2)3-imidazol-l-yl, -NH-(CH2)3-morpholin-4- yl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2-(1,3- dioxoisoindolin-1-yl), -NH-(CH2)2-(3,4-methylenedioxyphenyl), -NH-(CH2)2-phenyl, - NH-(CH2)2-thiophen-2-yl, -NH-(CH2)2-(3,4-ethylenedioxyphenyl), -S-(CH2)4-(1 ,3- dioxoisoindolin-1-yl), -NH(CH2)3-(2-oxo-pyrrolidin-l-yl), and -NH(CH2)2-(3,4,- dimethoxyphenyl) ;
(vii) when R is 3-methylphenyl, then R is not -O-(CH2)2-O-(phenyl);
(viii) when R is 3-thiophen-2-yl, then R is not chosen from -NH-(CH2)2- morpholin-4-yl, -NH-(CH2)3-moφholin-4-yl, -NH-(CH2)2-phenyl, and -NH-(CH2)2-(3,4- dimethoxyphenyl) ;
(ix) when R1 is 4-methoxyphenyl, then R2 is not chosen from -S-(CH2)3- phenyl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-C(CH3)2-moφholin-4-yl, - NHCH2C(CH3)2-moφholin-4-yl, -N(CH3)-(CH2)2-(3 ,4-dimethoxyphenyl), -S-(CH2)2- phenyl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(x) when R1 is 3,4-dimethylphenyl, then R2 is not chosen from -O-p- phenylene-O-CH2-phenyl, -S-(CH2)3-phenyl, -S-(CH2)3-moφholin-4-yl, -S-(CH2)2- phenyl, -NH-(CH2)3-(4-hydroxyρhenyl), -O-(CH2)2-(l,3-dioxoisoindolin-l-yl), -NH- (CH2)2- moφholin-4-yl, -NH-(CH2)3- moφholin-4-yl, -NH-(CH2)2-(3,4- methylenedioxyphenyl), -S-(CH2)2-phenyl, -NH-(CH2)2-phenyl, -NH-(CH2)2-thiophen-2- yl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(xi) when R1 is 4-bromophenyl, then R2 is not chosen from -(CH2)3-phenyl, -S- (CH2)3-morpholin-4-yl, -S-(CH2)2-phenyl, -S-(CH2)3-phenyl, -NH-(CH2)3-morpholin-4- yl, -NH-(CH2)3-(2-oxo-pyrrolidin-l-yl), and -NH-(CH2)2-(3,4-ethylenedioxyphenyl); and
(xii) when R1 is 4-biphenyl, then R2 is not -NH-(CH2)2-(3,4- ethylenedioxyphenyl);
(xiii) when R1 is 2,3-dihydro-l ,4-benzodioxin-2-yl, then R2 is not NH(CH2)2- phenyl; and
(xiv) 5-(2-chlorophenyl)-4-[4-(phenylmethoxy)phenoxy]-thieno[2,3- d]pyrimidine.
[044] In certain embodiments, R1 is chosen from aryl, substituted aryl, heterocyclyl, substituted heterocyclyl, heteroaryl, and substituted heteroaryl. [045] In certain embodiments, R1 is chosen from aryl and substituted aryl wherein substituted aryl is chosen from mono-, di-, and tri-substituted aryl. In certain embodiments, R1 is chosen from phenyl and substituted phenyl wherein substituted phenyl is chosen from mono-, di-, and tri-substituted phenyl. In certain embodiments, R1 is chosen from phenyl and phenyl substituted with one or more (such as one, two, or three) halo (such as fluoro). In some embodiments, R1 is chosen from phenyl and 4-F- phenyl.
[046] In certain embodiments, R1 is chosen from heterocyclyl, substituted heterocyclyl, heteroaryl, and substituted heteroaryl. hi certain embodiments, R1 is chosen from optionally substituted furanyl (e.g., optionally substituted furan-2-yl), optionally substituted pyridinyl (e.g., pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, any of which is optionally substituted), optionally substituted pyrimidinyl (e.g., optionally substituted 5- pyrimidin-2-yl), optionally substituted pyrazinyl (e.g., optionally substituted 5-pyrazin-2- yl), and optionally substituted thiazolyl (e.g., optionally substituted l,3-thiazol-2-yl). [047] In certain embodiments, R1 is chosen from optionally substituted furanyl
(e.g., optionally substituted furan-2-yl), optionally substituted pyridinyl (e.g., pyridin-4- yl, pyridin-3-yl, pyridin-2-yl, any of which is optionally substituted), and optionally substituted thiazolyl (e.g., optionally substituted l,3-thiazol-2-yl). [048] In certain embodiments, R1 is chosen from 5-chloro-furan-2-yl, furan-2-yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, l,3-thiazol-2-yl, 5-pyrimidin-2-yl and 5-pyrazin-
2-yl.
[049] In certain embodiments, R1 is chosen from furan-2-yl, pyridin-4-yl, pyridin-3-yl, l,3-thiazol-2-yl, and 5-pyrimidin-2-yl.
[050] In certain embodiments, R2 is chosen from -O-arylene-Y-R5, -
X(CR3R4)nYR5 and -(CR6R7)mYR5.
[051] In certain embodiments, R2 is -O-arylene-Y-R5. In certain embodiments,
R2 is -O-phenylene-Y-R5. In certain embodiments, the -O and -YR5 substituents attached the phenylene group are meta-substituted.
[052] In certain embodiments, R2 is -X(CR3R4)nY-R5.
[053 ] In certain embodiments, R2 is -(CR6R7)mYR5.
[054] In certain embodiments, X is -NH-. In certain embodiments, X is -O-.
[055] In certain embodiments, Y is a bond. In certain embodiments, Y is -O-.
In certain embodiments, Y is -NH-.
[056] In certain embodiments, n is chosen from 3, 4, and 5. In certain embodiments, n is chosen from 2 and 3. In certain embodiments, n is 3. In certain embodiments, n is 2.
[057] In certain embodiments, m is chosen from 3, 4, and 5.
[058] In certain embodiments, R3 and R4 are independently chosen from H,
CH3, and F. In certain embodiments, R3 and R4 are H.
[059] In certain embodiments, R6 and R7 are independently chosen from H, CH3, and F. In certain embodiments, R6 and R7 are H;
[060] In certain embodiments, -(CR6R7)mY-R5 is -(CH2)4-O-R5.
[061] In certain embodiments, -X(CR3R4)nY-R5 is chosen from -NH-(CH2)3-O-
R5, -NH-(CH2)2-NH-R5, -O-(CH2)2-O-R5, -O-(CH2)3-O-R5, -O-CH2-C(CH3)2-CH2-O-R5, and-NH-(CH2)2-O-R5. In certain embodiments, -X(CR3R4)nY-R5 is chosen from -NH-
(CH2)3-O-R5, -NH-(CH2)2-NH-R5, -O-(CH2)3-O-R5, and-NH-(CH2)2-O-R5. In certain embodiments, -X(CR3R4)πY-R5 is chosen from -NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5.
[062] In certain embodiments, -X(CR3R4)nYR5 is -NH-(CH2)3-R5 (i.e. Y is a covalent bond). [063] In certain embodiments, R9 is chosen from H, C1-4 alkyl; and C3-8 cycloalkyl, substituted C)-4 alkyl chosen from mono-, di-, and tri-substituted Cr4 alkyl; and substituted C3-8 cycloalkyl chosen from mono-, di-, and tri-substituted C3-8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo.
[064] In certain embodiments, R5 is chosen from pyridin-3-yl and pyridin-4-yl, each of which is optionally substituted with one or two groups chosen from
(1) C1-S alkyl,
(2) C3-S cycloalkyl,
(3) C4-g cycloalkylalkyl,
(4) C,-8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro,
(12) C1-S halogenated alkyl,
(13) C1-S halogenated alkoxy,
(14) Ci-8hydroxyalkyl,
(15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) C 1-8 alkylamino,
(18) di-C 1-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl, (25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-s alkylthio,
(37) Ci-8 alkylsulphinyl,
(38) C i -8 alkylsulphonyl,
(39) Ci-s alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-M aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cr8 alkyl, Q-4 alkoxy, halogenated Cp4 alkoxy, Ci-8 hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Cr8 alkylamino, di-Cr8 alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C i-8 alkyl, halogenated Ci -8 alkyl, Cp4 alkoxy, halogenated Q-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-g alkoxy, amino, d-8 alkylamino, di-Ci-8 alkylamino, Ci-g alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cp8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cp8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
[065] In certain embodiments, R5 is chosen from pyridin-2-yl and pyridin-4-yl, each of which is substituted with one or two groups chosen from (1) Cp8 alkyl, (2) C3-8 cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-S cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(H) nitro,
(12) C2-8 halogenated alkyl,
(13) Ci-8halogenated alkoxy,
(14) d-s hydroxyalkyl,
(15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) Cr8 alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-, (33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-g alkylthio,
(37) Ci-g alkylsulphinyl,
(38) Ci-g alkylsulphonyl,
(39) Ci-8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-20 aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-2o aryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Ci-8 alkyl, C1-4 alkoxy, halogenated Q-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, C)-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, C]-4 alkoxy, halogenated Q-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, d-8 alkylamino, di-Cj-g alkylamino, C1^ alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated d-8 alkyl, Ci-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-g alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Ci-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, d-8 alkyl, halogenated Crs alkyl, Cj-4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, Cr8 alkylsulphinyl, Cr8 alkylsulphonyl, Q-s alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cj-8 alkyl, Cr4 alkoxy, halogenated Q-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Q-8 alkylamino, di-Crs alkylamino, C1-S alkylsulphinyl, Crs alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
[066] Provided is at least one chemical entity chosen from compounds of Formula II:
Figure imgf000056_0001
(Formula II) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1, X, Y, n, R3, R4, and R9 are as described for compounds of Formula I and wherein p is chosen from 1, 2, 3, and 4; for each occurrence, R10 is independently chosen from
(1) Cj-g alkyl,
(2) C3-S cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) C3-8 cycloalkoxy,
(5) C4-8 cycloalkylalkoxy,
(6) amino,
(7) cyano,
(8) nitro,
(9) Ci-ghalogenated alkyl,
(10) Ci-ghalogenated alkoxy,
(11) Ci-8 hydroxyalkyl,
(12) C2-8 hydroxyalkoxy,
(13) C1-S alkylamino,
(14) di-d-salkylamino,
(15) carboxy,
(16) alkoxycarbonyl,
(17) aminocarbonyl,
(18) hydroxyaminocarbonyl,
(19) alkylaminocarbonyl,
(20) dialkylaminocarbonyl,
(21) urea,
(22) hydroxyurea,
(23) alkylurea,
(24) dialkylaminoalkylcarbonyl,
(25) aminocarbonylalkylaminocarbonyl,
(26) acylamido,
(27) HCO-NH-NH-CO-, (28) NH2NHCO-,
(29) NH2NHCONH-,
(30) acyloxy,
(31) Ci-β alkylthio,
(32) Ci-8 alkylsulphinyl,
(33) Ci-g alkylsulphonyl,
(34) Q-8 alkylsulphonamido,
(35) (alkylsuphonyl)2amino-,
(36) thiol,
(37) aminosulfonyl,
(38) alkylaminosulfonyl,
(39) C6-20 aryl,
(40) substituted C6-2o aryl chosen from mono-, di-, and tri-substituted C6-2o aryl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Ci-g alkyl, Ci-4 alkoxy, halogenated Cr4 alkoxy, Ci-8 hydroxyalkyl, C2-s hydroxyalkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(41) heterocyclyl-carbonyl,
(42) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, C1-S alkyl, halogenated Ci-8 alkyl, Cj-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Ci-8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(43) heteroaryl-carbonyl, and
(44) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Ci-8 alkyl, halogenated Cr8 alkyl, Cj-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cr8alkylamino, Ci-g alkylsulphinyl, d-8 alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio. [067] In certain embodiments,
R1 is chosen from aryl (such as phenyl), substituted aryl chosen from mono-, di-, and trisubstituted aryl wherein the substitutent is halo (such as F, e.g., phenyl substituted by fluoro), heterocyclyl, and substituted heterocyclyl, such as optionally substituted heterocyclyl chosen from optionally substituted furanyl, optionally substituted pyridinyl, optionally substituted pyrimidinyl, optionally substituted pyrazinyl, and optionally substituted thiazolyl, such as heterocyclyl chosen from 5-chloro-furan-2- yl, furan-2-yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, l,3-thiazol-2-yl, 5- pyrazin-2-yl, and 5-pyrimidin-2-yl; R2 is -X(CR3R4)nY-R5;
-X(CR3R4)nY- is chosen from -NH-(CH2)3-O-, -NH-(CH2)2-NH-, -O-(CH2)2-O-, -O- (CHb)3-O-, -O-CH2-C(CH3)2-CH2-O-, and -NH-(CH2)2-O-, and in certain embodiments, -X(CR3R4)nY- is chosen from -NH-(CH2)3-O- and -O-(CH2)3-O-; R3, R4, R6, and R7 are independently chosen from H, CH3, and F, and in certain embodiments, R3, R4, R6, and R7 are H;
X is chosen from O and NR9, and in certain embodiments, X is chosen from O and NH, Y is chosen from O and NR9, and in certain embodiments, Y is chosen from O and NH, and in yet other certain embodiments, Y is O; and R5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzyl chosen from mono-, di-, and tri-substituted benzyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl,
substituted benzoyl chosen from mono-, di-, and tri-substituted benzoyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzothiadiazolyl chosen from mono-, di-, and tri-substituted benzothiadiazolyl wherein the substituents are independently chosen from alkyl- CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2- CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH- CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene- NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted pyrazolyl chosen from mono-, di-, and tri-substituted pyrazolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzisothiazolyl chosen from mono-, di-, and tri-substituted benzisothiazolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH- CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alky^NH-alkylene-NH-CO-^alkyl^N-alkylene-NH-CO-^Hz-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted dihydroindolyl chosen from mono-, di-, and tri-substituted dihydroindolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH- CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyONH-alkylene-NH-CO-^alkyOzN-alkylene-NH-CO-^^-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, and substituted pyridinyl chosen from mono-, di-, and tri-substituted pyridinyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl; and in certain embodiments, R5 is chosen from phenyl and substituted phenyl wherein substituted phenyl is chosen from mono-, di-, and tri-substituted phenyl and wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3- CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH- CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO- NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
[068] In certain embodiments,
R1 is chosen from aryl (such as phenyl), aryl (such as phenyl) substituted by halo (such as F), and heterocyclyl, substituted heterocyclyl, such as optionally substituted heterocyclyl chosen from optionally substituted furanyl, optionally substituted pyridinyl, and optionally substituted thiazolyl, such as heterocyclyl chosen from fiιran-2- yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, and l,3-thiazol-2-yl;
R2 is -X(CR3R4)nY-R5;
-X(CR3R4)nY- is chosen from -NH-(CH2)3-O-, -NH-(CH2)2-NH-, -O-(CH2)3-O-, and-NH- (CH2)2-O-, and in certain embodiments, -X(CR3R4)nY- is chosen from -NH- (CH2)3-O- and -O-(CH2)3-O-;
R3, R4, R6, and R7 are independently chosen from H, CH3, and F, and in certain embodiments, R3, R4, R6, and R7 are H;
X is chosen from O and NR9, and in certain embodiments, X is chosen from O and NH;
Y is chosen from O and NR9, and in certain embodiments, Y is chosen from O and NH, and in yet other certain embodiments, Y is O; the subscript n is chosen from 2 and 3, and in certain embodiments, the subscript n is 3;
R5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzyl chosen from mono-, di-, and tri-substituted benzyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzoyl chosen from mono-, di-, and tri-substituted benzoyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzothiadiazolyl chosen from mono-, di-, and tri-substituted benzothiadiazolyl wherein the substituents are independently chosen from alkyl- CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2- CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH- CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene- NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted pyrazolyl chosen from mono-, di-, and tri-substituted pyrazolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzisothiazolyl chosen from mono-, di-, and tri-substituted benzisothiazolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH- CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyONH-alkylene-NH-CO-^alkyl^N-alkylene-NH-CO-^Hz-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted dihydroindolyl chosen from mono-, di-, and tri-substituted dihydroindolyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH- CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, and substituted pyridinyl chosen from mono-, di-, and tri-substituted pyridinyl wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH- CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl; and in certain embodiments, R5 is chosen from phenyl and substituted phenyl wherein substituted phenyl is chosen from mono-, di-, and tri-substituted phenyl and wherein the substituents are independently chosen from alkyl-CO-NH- (e.g., CH3- CO-NH-, CH3CH2-CO-NH- and cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH- CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO- NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl (e.g., ethyl), alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
[069] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, pyrimidinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, substituted pyrimidinyl, and substituted thiazolyl, and R2 is -X(CR3R4)nYR5. [070] In certain embodiments, R1 is chosen from furanyl, pyridinyl, thiazolyl, phenyl, and phenyl substituted by halo, and R2 is -X(CR3R4)nYR5. [071] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo, and R2 is -X(CR3R4)nYR5.
[072] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl, and R2 is chosen from -NH-(CH2)3-O-R5, -NH-(CH2)2-NH-R5, -O-(CH2)3-O-R5, and -NH-(CH2)2-O-R5.
[073] In certain embodiments, R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R2 is chosen from -NH-(CH2)3-O-R5, -NH- (CH2)2-NH-R5, -O-(CH2)3-O-R5, and -NH-(CH2)2-O-R5.
[074] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo, and R2 is chosen from -NH-(CH2)3-O-R5, -NH-(CH2)2-NH-R5, -O-(CH2)3-O-R5, and -NH-(CH2)2-O-R5.
[075] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, or thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl, and R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5. [076] In certain embodiments, R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R2 is chosen from NH-(CH2)3-O-R5 and -O-
(CH2)3-O-RS.
[077] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo, and R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5.
[078] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, which in each case is unsubstituted or substituted, and R is -O-(CH2)3-O-R .
[079] In certain embodiments, R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R2 is -O-(CH2)3-O-R5.
[080] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo, and R2 is -O-(CH2)3-O-R5.
[081] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substitutedthiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5; and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
[082] In certain embodiments, R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-
(CH2)3-O-R5, and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, and pyridinyl,substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
[083] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo; R2 is chosen from NH-(CH2)3-O-R5 or -O-(CH2)3-O-R5; and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
[084] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g.,
CH3-CO-NH-, CH3CH2-CO-NH- or cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH- CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH- , (alkyl-SO2)2N -, nitro, alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl. [085] In certain embodiments, R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O- (CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- or cyclopropyl-CO- NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2- CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH- CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
[086] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5; and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH3-CO- NH-, CH3CH2-CO-NH- or cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH- CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N- alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl- SO2)2N -, nitro, alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyloxy-carbonyl, and morpholinylcarbonyl.
[087] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo; R2 is NH-(CH2)2-O-R5, and R5 is chosen from phenyl and phenyl substituted by alkyl-CO-NH-. [088] In certain embodiments, R1 is chosen from phenyl and phenyl substituted by halo; R2 is NH-(CH2)3-O-R5, and; R5 is chosen from benzyl, benzoyly, and benzothiadiazolyl, substituted benzyl, substituted benzoyly, and substituted benzothiadiazolyl, which in each case the substituents are chosen from nitro, alkoxy (e.g., methoxy), cyano, halogenated alkoxy (e.g., OCF3), alkoxycarbonyl (e.g., methoxycarbonyl), and alkyl-CO-NH- (e.g., acetamido).
[089] In certain embodiments, R1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl; R is -O-(CH2)3-O-R , and R is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- or cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH- CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2- alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2- alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyl- oxycarbonyl, and morpholinylcarbonyl.
[090] In certain embodiments, R1 is chosen from phenyl and halogenated phenyl; R2 is -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH- (e.g., CH3-CO-NH-, CH3CH2-CO-NH- or cyclopropyl-CO-NH-), NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH- CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2- alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2- alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkoxy (e.g., methoxy), halogenated alkoxy (e.g., trifluoromethoxy), cyano, alkoxycarbonyl (e.g., methoxycarbonyl), triazolyl, halo (e.g., F), amino, carboxy, methylpiperazinyl- oxycarbonyl, and morpholinylcarbonyl.
[091] In certain embodiments, the compound of Formula I is chosen from:
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl]-acetamide,
N-(3-nitrobenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2-diamine, N-(3,4-dimethoxybenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2- diamine,
N-(2,l,3-benzothiadiazol-5-ylmethyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4- yl)ethane- 1 ,2-diamine,
N- {3-[( {2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl} amino)methyl]- phenyl } -acetamide,
N-{4-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- phenyl } -acetamide,
3-(acetylamino)-N-{2-[(5-phenylthieno[2,3-d]pyrimidm-4-yl)amino]- ethyl}benzamide,
3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- benzonitrile,
Methyl 3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)- methyl]-benzoate,
N-(5-phenylthieno[2,3-d]pyrimidin-4-yl)-N'-[3-(trifluoromethoxy)benzyl]ethane- 1,2-diamine,
5-(4-fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3-d]pyrimidine,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl]-acetamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide,
5-(4-fluorophenyl)-4- {3 - [4-( IH-1 ,2,4-triazol- 1 -yl)phenoxy]propoxy } thieno [2,3 - d]pyrimidine,
4-[3-(4-fluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine,
Methyl 3-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzoate,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)aniline,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoic acid,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- cyclopropanecarboxamide, 5-(4-fluorophenyl)-4-(3-{3-[(4-methylpiperazin-l-yl)carbonyl]phenoxy}- propoxy)-thieno[2,3-d]pyrimidine,
5-(4-fluorophenyl)-4- {3 - [3 -(morpholin-4-ylcarbonyl)phenoxy]propoxy} - thieno[2,3-d]pyrimidine,
N-ethyl-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N,N- dimethylbenzamide,
N-[2-(dimethylamino)ethyl]-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzamide,
N-(2-amino-2-oxoethyl)-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N'- formylbenzohydrazide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- methylbenzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzamide,
3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)-N- methylbenzamide,
N-[3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)phenyl]- urea,
N-[3 -(3 - { [5-(4-fluorophenyl)thieno [2,3 -d]pyrimidin-4-yl] oxy } propoxy)- phenyl]urea,
3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzohydrazide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- hydroxyb enzamide,
4-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)benzamide
N-ethyl-N'-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl] oxy } propoxy)phenyl]urea, N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- methanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- methanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N-(methylsulfonyl)methansulfonamide,
N-(ethylsulfonyl)-N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}- propoxy)phenyl]ethanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- ethanesulfonamide,
N- [4-(3 - { [ 5 -(4-fluorophenyl)thieno [2,3 -d]pyrimidin-4-yl] oxy } propoxy)phenyl] - N'-methylurea,
N-ethyl-N'-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]-urea,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- ethanesulfonamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-methylurea,
N-[3-(3-{[5 -(4-fluorophenyl)thieno [2,3 -d]pyrimidin-4-yl] oxy} propoxy)phenyl] - hydrazinecarboxamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-hydroxyurea,
4-{3-[5-(4-fluorophenyl)-thieno[2,3d]pyrimidin-4-yloxy]-propoxy}-benzamide,
5-{3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid, methyl ester,
N-{3-[3-(5-furan-2-yl-thieno[2,3d]pyrimidin-4-yloxy)-propoxy]- phenyl } acetamide,
2-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}proρoxy)benzamide,
5-{3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid,
N-(3-{3-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}phenyl)acetamide, N-[4-(3-{[5-(4-fluorophenyl)mieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- hydrazinecarboxamide,
5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide,
5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide
N-methyl-5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- pyridine-2-carboxamide,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- methylpyridine-2-carboxamide,
N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- pyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- pyridine-2-carboxamide, [092] In certain embodiments, the compound of Formula I is chosen from:
N-methyl-3 - {3 - [(5-pyridin-3 -ylthieno[2,3 -d]pyrimidin-4- yl)oxy]propoxy}benzamide,
N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)-thieno[2,3-d]ρyrimidin-4-yl]amino}- propoxy)pyridine-2-carboxamide,
5-(3-{[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)-N- methylpyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4- yl)amino]propoxy}pyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4- yl)amino]propoxy}pyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}- nicotinamide, 5-{3-[(5-pyridin-3-ylthieno[2,3-d]-pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide hydroformate,
5-(4-fluorophenyl)-4-(3-{[l-methyl-5-(trifluoromethyl)-lH-pyrazol-3- yl]oxy}propoxy)thieno[2,3-d]pyrimidine,
4-[3-(4-chlorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine,
4-[3-(3,5-difluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine,
4-[3-(3,4-dimethoxyphenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3- d]pyrimidine,
4-[3-(l,2-benzisothiazol-3-yloxy)propoxy]-5-(4-fluorophenyl)thieno[2,3- djpyrimidine,
5-(4-fluorophenyl)-4-(3-phenoxypropoxy)-thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-[3-(3-methoxy-phenoxy)propoxy]thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-[3-(3-methyl-phenoxy)propoxy]thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-{3-[3-(trifluoro-methoxy)phenoxy]propoxy}thieno[2,3- d]pyrimidine,
4-[3-(3-ethylphenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, 5-{3-[(5-pyrimidin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine-
2-carboxylic acid hydroformate,
5-{3-[(5-pyrazin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate,
4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butan-l-ol,
4-but-3-en-l-yl-5-(4-fluorophenyl)-thieno[2,3-d]pyrimidine,
Methyl 5-{4-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yl]butoxy}-nicotinate,
5- {4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinic acid hydroformate,
5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy}-N- methylnicotinamide hydroformate,
5- {4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinamide hydroformate, N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}phenoxy)- phenyl]acetamide hydro formate,
N-[3-(2,2-dimethyl-3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl] acetamide hydro formate,
N-[3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}phenoxy)phenyl]- acetamide hydroformate,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}-2,2-dimethyl- propoxy)phenyl] acetamide hydroformate,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-l,3-dihydro- 2H-indol-2-one,
5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxamide hydroformate,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine- 2-carboxamide hydroformate,
N-[4-(3-{ [5 -(4-fluorophenyl)thieno [2,3 -d]pyrimidin-4-yl] amino } propyl)phenyl] - acetamide,
N-(3-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[3 -(3 - { [5-( 1 ,3 -thiazol-2-yl)thieno[2,3 -d]pyrimidin-4-yl] amino }propoxy)- phenyl] acetamide,
N-(3-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[4-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] acetamide,
N-(4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[4-(3-{[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] acetamide,
5 -(4-fluorophenyl)-4- [3 -(3 -nitrophenoxy)propoxy] thieno [2 , 3 -d] pyrimidine,
N-methyl-4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- benzamide, N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- nicotinamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- nicotinamide,
N-methyl-3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide,
N-[4-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide, and
N-(4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}phenyl)- acetamide.
[093] In certain embodiments, the compound of Formula I is chosen from those listed in Table I:
Table 1
Cpd # Structure Name
N-(3-{3-[(5-phenylthieno[2,3-d]pyrimidin- 4-yl)amino]-propoxy}phenyl)-acetamide
Figure imgf000077_0001
N-[3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl] amino } - propoxy)phenyl] -acetamide
Figure imgf000077_0002
N-(3-nitrobenzyl)-N'-(5-phenylthieno[2,3- d]pyrimidin-4-yl)ethane- 1 ,2-diamine
N-(3,4-dimethoxybenzyl)-N'-(5- phenylthieno[2,3-d]pyrimidin-4-yl)ethane-
Figure imgf000077_0003
1 ,2-diamine N-(2, 1 ,3-benzothiadiazol-5-ylmethyl)-N'- (5 -phenylthieno [2 , 3 -d] pyrimidin-4-
Figure imgf000078_0001
yl)ethane-l ,2-diamine
N-{3-[({2-[(5-phenylthieno[2,3- d]pyrimidin-4-yl)amino] ethyl } -
Figure imgf000078_0002
amino)methyl]phenyl } acetamide
N-{4-[({2-[(5-phenylthieno[2,3- d]pyrimidin-4-yl)amino] ethyl } amino)- methyl]phenyl } acetamide
Figure imgf000078_0003
3-(acetylamino)-N- {2-[(5- phenylthieno[2,3-d]pyrimidin-4- yl)amino] ethyl }benzamide
Figure imgf000078_0004
3-[({2-[(5-phenylthieno[2,3-d]pyrimidin- 4-yl)amino] ethyl }-amino)-
CO methyl]benzonitrile
methyl 3-[({2-[(5-phenylthieno[2,3- d]pyrimidin-4-yl)amino]ethyl}- amino)methyl]benzoate
Figure imgf000078_0005
N-(5-phenylthieno[2,3-d]pyrimidin-4-yl)- N'-[3-(trifluoromethoxy)-benzyl]-ethane- 1 ,2-diamine
Figure imgf000078_0006
5-(4-fluorophenyl)-4-[3-(3- nitrophenoxy)propoxy]thieno[2,3- djpyrimidine
Figure imgf000079_0001
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl] amino } - propoxy)phenyl] acetamide
Figure imgf000079_0002
N-[3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl] oxy} propoxy)- phenyl] acetamide
Figure imgf000079_0003
N-[4-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl] oxy } propoxy)- phenyl] acetamide
Figure imgf000079_0004
5-(4-fluorophenyl)-4-{3-[4-(lH-l,2,4- triazol- 1 -yl)phenoxy] -propoxy} -
Figure imgf000079_0005
thieno [2,3 -djpyrimidine
4-[3-(4-fluorophenoxy)propoxy]-5-(4- fluorophenyl)thieno[2,3-d]pyrimidine
Figure imgf000079_0006
methyl 3-(3-{[5-(4-fluorophenyl)- thieno[2,3-d]pyrimidin-4-yl]oxy}- propoxy)benzoate
Figure imgf000079_0007
-
Figure imgf000080_0001
3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d] pyrimidin-4-yl] oxy } propoxy)-N'- formylbenzohydrazide
3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)-N-methyl-
Figure imgf000081_0001
benzamide
3-(3-{[5-(4-fluorophenyl-)thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)-benzamide
3-(2-{[5-(4-fluorophenyl)-thieno[2,3- d] pyrimidin-4-yl] oxy} ethoxy)-N-
Figure imgf000081_0002
methylbenzamide
N-[3-(2-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}ethoxy)phenyl]urea
N-[3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4- yl]oxy}propoxy)ρhenyl]urea
3-(2-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}ethoxy)-benzamide
3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)-
Figure imgf000081_0003
benzohydrazide 3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d] pyrimidin-4-yl] oxy } propoxy)-N-
Figure imgf000082_0001
hydroxy-benzamide
4-(2-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl] oxy} ethoxy)-benzamide
Figure imgf000082_0002
N-ethyl-N'-[3-(3-{[5-(4- h / H1C-[Ao fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)-phenyl]urea
N-[3-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)- phenyl]methane-sulfonamide
N-[4-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy} -
Figure imgf000082_0003
propoxy)phenyl]methansulfonamide
N-[4-(3-{[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)-phenyl]- N-(methylsulfonyl)methansulfonamide
Figure imgf000082_0004
N-(ethylsulfonyl)-N-[4-(3-{[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-
OT yl]oxy}propoxy)phenyl]ethanesulfonamide
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)- phenyl] ethanesulfonamide
Figure imgf000082_0005
44 N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl] oxy } propoxy)-phenyl] - N'-methylurea
Figure imgf000083_0001
45 N-ethyl-N'-[4-(3-{[5-(4-fluoro- phenyl)thieno [2 ,3 -d] pyrimidin-4-yl] oxy} - propoxy)phenyl]urea
46 N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)- phenyl] ethanesulfonamide
Figure imgf000083_0002
47 N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl] oxy} propoxy)-phenyl] - N'-methylurea
Figure imgf000083_0003
48 N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)- phenyl]hydrazinecarboxamide
49 N-[3-(3-{[5-(4-fluoroρhenyl)thieno[2,3- d]pyrimidin-4-yl]oxy}propoxy)-phenyl]-
N'-hydroxyurea
50 4-{3-[5-(4-fluorophenyl)-thieno[2,3d]- pyrimidin-4-yloxy] -propoxy } -b enzamide
51 5-{3-[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yloxy] -propoxy} -nicotinic acid methyl ester
52 N-{3-[3-(5-furan-2-yl-thieno[2,3d]- pyrimidin-4-yloxy)-propoxy] -
Figure imgf000083_0004
phenyl }acetamide
Figure imgf000084_0001
Figure imgf000085_0001
Figure imgf000086_0001
Figure imgf000087_0001
Figure imgf000088_0001
Figure imgf000089_0001
Figure imgf000090_0001
Figure imgf000091_0001
[094] Also provided are s pharmaceutical compositions comprising at least one chemical entity described herein and a pharmaceutically acceptable carrier and, optionally, another active agent as discussed below. Also provided is a method of inhibiting a PDElO enzyme, e.g., as determined by a conventional assay or one described herein, either in vitro or in vivo (in an animal, e.g., in an animal model, or in a mammal or in a human); a method of treating a psychiatric or neurological syndrome, e.g., pyschoses, obsessive-compulsive disorder and/or Parkinson's disease; a method of treating a disease state modulated by PDElO activity, in a mammal, e.g., a human, e.g., those disease states mentioned herein.
[095] Methods include, but are not limited to, methods of enhancing cognition in a patient in whom such enhancement is desired, methods of treating a patient suffering from cognition impairment or decline, methods of treating a patient having a disease involving decreased cAMP and/or cGMP levels, methods of inhibiting PDElO enzyme activity in a patient, methods of treating a patient suffering psychoses, in particular schizophrenia or bipolar disorder, methods of treating a patient suffering from obsessive- compulsive disorder, methods of treating a patient suffering from Parkinson's disease. In certain embodiments, the disease state is one that involves elevated intracellular PDElO levels or decreased cAMP and/or cGMP levels, e.g., involving neurological or psychiatric syndromes, especially those states associated with psychoses, most especially schizophrenia or bipolar disorder, obsessive-compulsive disorder, and/or Parkinson's disease. All methods comprise administering to the patient an effective amount of at least one chemical entity described herein. In certain embodiments, the patient is human. [096] Chemical entities described herein can be made by the methods depicted in the reaction schemes shown below.
[097] The starting materials and reagents used in preparing these compounds are either available from commercial suppliers such as Aldrich Chemical Co., (Milwaukee, Wis.), Bachem (Torrance, Calif.), or Sigma (St. Louis, Mo.) or are prepared by methods known to those skilled in the art following procedures set forth in references such as Fieser and Fieser's Reagents for Organic Synthesis, Volumes 1-17 (John Wiley and Sons, 1994); Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989); Organic Reactions, Volumes 1-40 (John Wiley and Sons, 2003), March's Advanced Organic Chemistry, (John Wiley and Sons, 4th Edition) and Larock's Comprehensive Organic Transformations (VCH Publishers Inc., 1989). Those schemes are merely illustrative of some methods by which the chemical entities described herein can be synthesized, and various modifications to these schemes can be made and will be suggested to one skilled in the art having referred to this disclosure. [098] The starting materials and the intermediates of the reaction may be isolated and purified if desired using conventional techniques, including but not limited to filtration, distillation, crystallization, chromatography and the like. Such materials may be characterized using conventional means, including physical constants and spectral data.
[099] Unless specified to the contrary, the reactions described herein take place at atmospheric pressure over a temperature range from about -78 0C to about 150 0C, such as from about 0 0C to about 125 0C, for example, at about room (or ambient) temperature, e.g., about 20 0C.
[0100] Compounds of Formula I can be prepared as shown in Scheme 1 below.
Scheme 1
formamide
Figure imgf000093_0001
[0101] Treatment of a mixture of a methyl ketone compound of formula 1 with cyanoacetic acid ethyl ester and sulfur with morpholine in a suitable alcoholic solvent such as ethanol provides a 2-amino-thiophene ethyl ester compound of formula 3. Reation of 3 with formamide provides a 4-hydroxythieneo[2,3-d]pyrimidine compound of formula 4. Compound 4 can then be converted to a compound of Formula I where R is -O(CR3R4)nYR5 by reacting 4 with LG(CR3R4)nYR5 where LG is a suitable leaving group such as halo under conditions well known in the art.
[0102] Alternatively, compound 4 can be first converted to a compound of formula 5 by treating with with a chlorinating agent such as thionyl chloride and the. Compound 5 is then converted to a compound of Formula I by treating it with is HO(CR3R4)nYR5, is HS(CR3R4)nYR5, or is NH2(CR3R4)nYR5 under conditions well known in the art. Detailed descriptions of such procedures are provided in the Working examples below.
[0103] Compounds of Formula I wherein R2 is -(CR3R4)nYR5 can be prepared by reaction of compound 5 with the Grignard BrMg-(CR3R4),, YR5 under anhydrous conditions in a polar aprotic solvent (such as THF) using CuI and/or FeBr2 as catalysts. [0104] The optical isomers of compounds of Formula I can be obtained by resolution of the racemic mixtures according to conventional processes, for example, by the formation of diastereomeric salts using an optically active acid or base or formation of covalent diastereomers. Examples of appropriate acids are tartaric, diacetyltartaric, dibenzoyltartaric, ditoluoyltartaric and camphorsulfonic acid. Mixtures of diastereomers can be separated into their individual diastereomers on the basis of their physical and/or chemical differences by methods known to those skilled in the art, for example, by chromatography or fractional crystallization. The optically active bases or acids are then liberated from the separated diastereomeric salts.
[0105] A different process for separation of optical isomers involves the use of chiral chromatography (e.g., chiral HPLC columns), with or without conventional derivation, optimally chosen to maximize the separation of the enantiomers. Suitable chiral HPLC columns are manufactured by Diacel, e.g., Chiracel OD and Chiracel OJ among many others, all routinely selectable. Enzymatic separations, with or without derivitization, are also useful. The optically active compounds of Formula I can likewise be obtained by utilizing optically active starting materials in chiral syntheses processes under reaction conditions which do not cause racemization.
[0106] The compounds can be used in different enriched isotopic forms, e.g., enriched in the content of 2H, 3H, 11C, 13C and/or 14C. In certain embodiments, the compounds are deuterated. Such deuterated forms can be made by the procedure described in U.S. Patent Nos. 5,846,514 and 6,334,997. As described in U.S. Patent Nos. 5,846,514 and 6,334,997, deuteration can improve the efficacy and increase the duration of action of drugs.
[0107] Deuterium substituted compounds can be synthesized using various methods such as described in: Dean, Dennis C; Editor. Recent Advances in the Synthesis and Applications of Radiolabeled Compounds for Drug Discovery and Development. [In: Curr., Pharm. Des., 2000; 6(10)] (2000), 110 pp. CAN 133:68895 AN 2000:473538 CAPLUS; Kabalka, George W.; Varma, Rajender S. The Synthesis of Radiolabeled Compounds via Organometallic Intermediates. Tetrahedron (1989), 45(21), 6601-21, CODEN: TETRAB ISSN:0040-4020. CAN 112:20527 AN 1990:20527 CAPLUS; and Evans, E. Anthony. Synthesis of radiolabeled compounds, J. Radioanal. Chem. (1981), 64(1-2), 9-32. CODEN: JRACBN ISSN:0022-4081, CAN 95:76229 AN 1981 :476229 CAPLUS.
[0108] The acid addition salts of the claimed compounds may be prepared by reaction of the compounds with the appropriate inorganic or organic acid via any of a number of known methods. Alternatively, alkali and alkaline earth metal salts are prepared by reacting the chemical entities described herein with the appropriate base via a variety of known methods. In certain embodiments, the salts formed are pharmaceutically acceptable for administration to mammals. However, pharmaceutically unacceptable salts of the compounds are suitable as intermediates, for example, for isolating the compound as a salt and then converting the salt back to the free base compound by treatment with an alkaline reagent. The free base can then, if desired, be converted to a pharmaceutically acceptable acid addition salt.
[0109] The chemical entities described herein can be administered alone or as an active ingredient of a formulation. Thus, also provided are pharmaceutical compositions comprising at least one chemical entity described herein and one or more pharmaceutically acceptable carriers.
[0110] The chemical entities described herein can be administered to anyone requiring PDElO inhibition. Administration may be accomplished according to patient needs, for example, orally, nasally, parenterally (subcutaneously, intravenously, intramuscularly, intrasternally and by infusion) by inhalation, rectally, vaginally, topically and by ocular administration. [0111] Various solid oral dosage forms can be used for administering chemical entities described herein including such solid forms as tablets, gelcaps, capsules, caplets, granules, lozenges and bulk powders. The chemical entities described herein can be administered alone or combined with various pharmaceutically acceptable carriers, diluents (such as sucrose, mannitol, lactose, starches) and excipients known in the art, including but not limited to suspending agents, solubilizers, buffering agents, binders, disintegrants, preservatives, colorants, flavorants, lubricants and the like. Time release ■ capsules, tablets and gels are also advantageous in administering the chemical entities described herein.
[0112] Various liquid oral dosage forms can also be used for administering chemical entities described herein, including aqueous and non-aqueous solutions, emulsions, suspensions, syrups, and elixirs. Such dosage forms can also contain suitable inert diluents known in the art such as water and suitable excipients known in the art such as preservatives, wetting agents, sweeteners, flavorants, as well as agents for emulsifying and/or suspending the chemical entities described herein. The chemical entities described herein may be injected, for example, intravenously, in the form of an isotonic sterile solution. Other preparations are also possible.
[0113] Suppositories for rectal administration of the chemical entities described herein can be prepared by mixing the compound with a suitable excipient such as cocoa butter, salicylates and polyethylene glycols. Formulations for vaginal administration can be in the form of a pessary, tampon, cream, gel, paste, foam, or spray formula containing, in addition to the active ingredient, such suitable carriers as are known in the art. [0114] For topical administration, the pharmaceutical composition can be in the form of creams, ointments, liniments, lotions, emulsions, suspensions, gels, solutions, pastes, powders, sprays, and drops suitable for administration to the skin, eye, ear or nose. Topical administration may also involve transdermal administration via means such as transdermal patches.
[0115] Aerosol formulations suitable for administering via inhalation also can be made. For example, for treatment of disorders of the respiratory tract, the chemical entities described herein can be administered by inhalation in the form of a powder (e.g., micronized) or in the form of atomized solutions or suspensions. The aerosol formulation can be placed into a pressurized acceptable propellant.
[0116] Numerous standard references are available that describe procedures for preparing various formulations suitable for administering the chemical entities described herein. Examples of potential formulations and preparations are contained, for example, in the Handbook of Pharmaceutical Excipients, American Pharmaceutical Association (current edition); Pharmaceutical Dosage Forms: Tablets (Lieberman, Lachman and Schwartz, editors) current edition, published by Marcel Dekker, Inc., as well as Remington's Pharmaceutical Sciences (Arthur Osol, editor), 1553-1593 (current edition). [0117] The chemical entities described herein can be administered as the sole active agent or in combination with other pharmaceutical agents such as other agents used in the treatment of psychoses, such as schizophrenia and bipolar disorder, obsessive- compulsive disorder, Parkinson's disease, cognitive impairment and/or memory loss, e.g., nicotinic α-7 agonists, PDE4 inhibitors, other PDElO inhibitors, calcium channel blockers, muscarinic ml and m2 modulators, adenosine receptor modulators, ampakines, NMDA-R modulators, mGluR modulators, dopamine modulators, serotonin modulators, canabinoid modulators, and cholinesterase inhibitors (e.g., donepezil, rivastigimine, and galanthanamine). In such combinations, each active ingredient can be administered either in accordance with their usual dosage range or a dose below their usual dosage range.
[0118] The chemical entities described herein can be administered in combination with other pharmaceutical agents used in the treatment of schizophrenia, e.g., Clozaril, Zyprexa, Risperidone, and Seroquel. Thus, also provided are methods for treating schizophrenia, including memory impairment associated with schizophrenia, comprising administering to a patient, simultaneously or sequentially, at least one chemical entities described herein and another agent used in the treatment of schizophrenia such as Clozaril, Zyprexa, Risperidone, and Seroquel. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of schizophrenia, e.g., Clozaril, Zyprexa, Risperidone, and Seroquel. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of schizophrenia, e.g., Clozaril, Zyprexa, Risperidone, and Seroquel. [0119] In addition, the chemical entities described herein can be administered in combination with other pharmaceutical agents used in the treatment bipolar disorder such as Lithium, Zyprexa, or Depakote. Thus, also provided are methods for treating bipolar disorder, including treating memory and/or cognitive impairment associated with the disease, comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of bipolar disorder such as Lithium, Zyprexa, or Depakote. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising a at least one chemical entity described herein and another pharmaceutical agent used in the treatment of bipolar disorder such as Lithium, Zyprexa, or Depakote. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of bipolar disorder such as Lithium, Zyprexa, or Depakote. [0120] Also provided are methods for treating Parkinson's disease, including treating memory and/or cognitive impairment associated with Parkinson's disease, comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described hereinand another agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described hereinand another pharmaceutical agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin. Similarly, also provided are kits comprising a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Parkinson's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin. [0121] In addition, also provided are methods for treating memory and/or cognitive impairment associated with Alzheimer's disease comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described hereinand another agent used in the treatment of Alzheimer's disease such as Reminyl, Cognex, Aricept, Exelon, Akatinol, Neotropin, Eldepryl, Estrogen and Cliquinol. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of Alzheimer's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Alzheimer's disease such as Levodopa, Parlodel, Permax, Mirapex, Tasmar, Contan, Kemadin, Artane, and Cogentin.
[0122] Also provided are methods for treating Huntington's disease, including treating memory and/or cognitive impairment associated with Huntington's disease, comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of Huntington's disease such as Amitriptyline, Imipramine, Despiramine, Nortriptyline, Paroxetine, Fluoxetine, Setraline, Terabenazine, Haloperidol, Chloropromazine, Thioridazine, Sulpride, Quetiapine, Clozapine, and Risperidone.
[0123] Also provided are methods for treating memory and/or cognitive impairment associated with dementia comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of dementia such as Thioridazine, Haloperidol, Risperidone, Cognex, Aricept, and Exelon.
[0124] Also provided are methods for treating memory and/or cognitive impairment associated with epilepsy comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are ompositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of epilepsy such as Dilantin, Luminol, Tegretol, Depakote, Depakene, Zarontin, Neurontin, Barbita, Solfeton, and Felbatol.
[0125] Also provided are methods for treating memory and/or cognitive impairment associated with multiple sclerosis comprising administering to a patient, simultaneously or sequentially, at least one chemical entity described herein and another agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone. In methods using simultaneous administration, the agents can be present in a combined composition or can be administered separately. As a result, also provided are compositions comprising at least one chemical entity described herein and another pharmaceutical agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone. Similarly, also provided are kits containing a composition comprising at least one chemical entity described herein and another composition comprising another pharmaceutical agent used in the treatment of multiple sclerosis such as Detrol, Ditropan XL, OxyContin, Betaseron, Avonex, Azothioprine, Methotrexate, and Copaxone.
[0126] Also provided are chemical entities that inhibit PDElO enzyme activity.
PDElO inhibitors will raise the levels of cAMP or cGMP within cells that express PDElO. Inhibition of PDElO enzyme activity may be of relevance to diseases caused by deficient amounts of c AMP or cGMP in cells. Alternatively, PDElO inhibitors may be of benefit in cases wherein raising the amount of cAMP or cGMP above normal levels results in a therapeutic effect. Inhibitors of PDElO may be used to treat disorders of the peripheral and central nervous system, cardiovascular diseases, cancer, gastroenterological diseases, endocrinological diseases and urological diseases. Thus, also provided are methods of selective inhibition of PDElO enzymes in animals, e.g., mammals, for example, humans, wherein such inhibition has a therapeutic effect, such as where such inhibition may relieve conditions involving neurological or psychiatric syndromes, such as the loss of memory or psychoses. Such methods comprise administering to an animal in need thereof, such as a mammal, for example, a human, an inhibitory amount of at least one chemical entity described herein , alone or as part of a formulation.
[0127] Indications that may be treated with PDE 10 inhibitors, either alone or in combination with other drugs, include, but are not limited to, those diseases thought to be mediated in part by the basal ganglia, prefrontal cortex and hippocampus. These indications include psychoses, Parkinson's disease, dementias, obsessive compulsion disorder, tardive dyskinesia, choreas, depression, mood disorders, impulsivity, drug addiction, attention deficit/hyperactivity disorder (ADHD), depression with parkinsonian states, personality changes with caudate or putamen disease, dementia and mania with caudate and pallidal diseases, and compulsions with pallidal disease. [0128] Psychoses are disorders that affect an individual's perception of reality.
Psychoses are characterized by delusions and hallucinations. Also provided are methods for treating patients suffering from all forms of psychoses, including but not limited to schizophrenia, late-onset schizophrenia, schizoaffective disorders, prodromal schizophrenia, and bipolar disorders. Treatment may be for the positive symptoms of schizophrenia as well as for the cognitive deficits and negative symptoms. Other indications for PDElO inhibitors are psychoses resulting from drug abuse (including amphetamines and PCP), encephalitis, alcoholism, epilepsy, Lupus, sarcoidosis, brain tumors, multiple sclerosis, dementia with Lewy bodies, or hypoglycemia. Other psychiatric disorders, like posttraumatic stress disorder (PTSD), and schizoid personality may also be treated with PDElO inhibitors.
[0129] Obsessive-compulsive disorder (OCD) has been linked to deficits in the frontal-striatal neuronal pathways. (Saxena S. et al., Br J Psychiatry Suppl. 1998;(35):26- 37.) Neurons in these pathways project to striatal neurons that express PDElO. PDElO inhibitors cause cAMP to be elevated in these neurons; elevations in cAMP result in an increase in CREB phosphorylation and thereby improve the functional state of these neurons. PDElO inhibitors should be useful for the indication of OCD. OCD may result, in some cases, from streptococcal infections that cause autoimmune reactions in the basal ganglia (Giedd J. N. et al., Am. J. Psychiatry. 2000 Feb;157(2):281-3). Because PDElO inhibitors may serve a neuroprotective role, administration of PDElO inhibitors may prevent the damage to the basal ganglia after repeated streptococcal infections and thereby prevent the development of OCD.
[0130] In the brain, the level of cAMP or cGMP within neurons is believed to be related to the quality of memory, such as long term memory. Without wishing to be bound to any particular mechanism, it is proposed that since PDElO degrades cAMP or cGMP, the level of this enzyme affects memory in animals, for example, in humans. For example, a compound that inhibits cAMP phosphodiesterase (PDE) can thereby increase intracellular levels of cAMP, which in turn activate a protein kinase that phosphorylates a transcription factor (cAMP response binding protein), which transcription factor then binds to a DNA promoter sequence to activate genes that are important in long term memory. The more active such genes are, the better is long-term memory. Thus, by inhibiting a phosphodiesterase, long term memory can be enhanced. [0131] Dementias are diseases that include memory loss and additional intellectual impairment separate from memory. Also provided are methods for treating patients suffering from memory impairment in all forms of dementia. Dementias are classified according to their cause and include: neurodegenerative dementias (e.g., Alzheimer's, Parkinson's disease, Huntingdon's disease, Pick's disease), vascular (e.g., infarcts, hemorrhage, cardiac disorders), mixed vascular and Alzheimer's, bacterial meningitis, Creutzfeld-Jacob Disease, multiple sclerosis, traumatic (e.g., subdural hematoma or traumatic brain injury), infectious (e.g., HIV), genetic (down syndrome), toxic (e.g., heavy metals, alcohol, some medications), metabolic (e.g., vitamin B12 or folate deficiency), CNS hypoxia, Cushing's disease, psychiatric (e.g., depression and schizophrenia), and hydrocephalus.
[0132] The condition of memory impairment is manifested by impairment of the ability to learn new information and/or the inability to recall previously learned information. Also provided are methods for dealing with memory loss separate from dementia, including mild cognitive impairment (MCI) and age-related cognitive decline. Also provided are methods of treatment for memory impairment as a result of disease. Memory impairment is a primary symptom of dementia and can also be a symptom associated with such diseases as Alzheimer's disease, schizophrenia, Parkinson's disease, Huntington's disease, Pick's disease, Creutzfeld-Jakob disease, HIV, cardiovascular disease, and head trauma as well as age-related cognitive decline. Also provided are methods for dealing with memory loss resulting from the use of general anesthetics, chemotherapy, radiation treatment, post-surgical trauma, and therapeutic intervention. Thus, in certain embodiments, also provided are methods of treating patients suffering from memory impairment due to, for example, Alzheimer's disease, multiple sclerosis, amylolaterosclerosis (ALS), multiple systems atrophy (MSA), schizophrenia, Parkinson's disease, Huntington's disease, Pick's disease, Creutzfeld-Jakob disease, depression, aging, head trauma, stroke, spinal cord injury, CNS hypoxia, cerebral senility, diabetes associated cognitive impairment, memory deficits from early exposure of anesthetic agents, multiinfarct dementia and other neurological conditions including acute neuronal diseases, as well as HIV and cardiovascular diseases. Also provided are agents and/or methods to stimulate the formation of memory in "normal" subjects (i.e., subjects who do not exhibit an abnormal or pathological decrease in a memory function), e.g., ageing middle-aged subjects. Lots of repetition, maybe you want to consolidate [0133] In certain embodiments, the chemical entities described herein are used in the treatment of a class of disorders known as polyglutamine-repeat diseases. These diseases share a common pathogenic mutation. The expansion of a CAG repeat, which encodes the amino acid glutamine, within the genome leads to production of a mutant protein having an expanded polyglutamine region. For example, Huntington's disease has been linked to a mutation of the protein huntingtin. In individuals who do not have Huntington's disease, huntingtin has a polyglutamine region containing about 8 to 31 glutamine residues. For individuals who have Huntington's disease, huntingtin has a polyglutamine region with over 37 glutamine residues. Aside from Huntington's disease (HD), other known polyglutamine-repeat diseases and the associated proteins are: dentatorubral-pallidoluysian atrophy, DRPLA (atrophin-1); spinocerebellar ataxia type-1 (ataxin-1); spinocerebellar ataxia type-2 (ataxin-2); spinocerebellar ataxia type-3 also called Machado-Joseph disease, MJD (ataxin-3); spinocerebellar ataxia type-6 (alpha la- voltage dependent calcium channel); spinocerebellar ataxia type-7 (ataxin-7); and spinal and bulbar muscular atrophy, SBMA, also know as Kennedy disease (androgen receptor). Thus, also provided are methods of treating a polyglutamine-repeat disease or CAG repeat expansion disease comprising administering to a patient, such as a human, a therapeutically effective amount of a compound. In certain embodiments, there is provided a method of treating Huntington's disease (HD), dentatorubral-pallidoluysian atrophy (DRPLA), spinocerebellar ataxia type-1, spinocerebellar ataxia type-2, spinocerebellar ataxia type-3 (Machado-Joseph disease), spinocerebellar ataxia type-6, spinocerebellar ataxia type-7, or spinal and bulbar muscular atrophy, comprising administering to a patient, such as a human, a therapeutically effective amount of a compound. [0134] The basal ganglia are important for regulating the function of motor neurons; disorders of the basal ganglia result in movement disorders. Most prominent among the movement disorders related to basal ganglia function is Parkinson's disease (Obeso JA et al., Neurology, 2004 Jan 13;62(1 Suppl l):S17-30). Other movement disorders related to dysfunction of the basla ganglia include tardive dyskinesia, progressive supranuclear palsy and cerebral palsy, corticobasal degeneration, multiple system atrophy, Wilson disease, and dystonia, tics, and chorea. In certain embodiments, PDElO inhibitors may be used to treat movement disorders related to dysfunction of basal ganglia neurons.
[0135] PDEl 0 inhibitors can be used to raise cAMP or cGMP levels and prevent neurons from undergoing apoptosis. PDElO inhibitors may be anti-inflammatory by raising cAMP in glial cells. The combination of anti-apoptotic and anti-inflammatory properties, as well as positive effects on synaptic plasticity and neurogenesis, make these compounds useful to treat neurodegeneration resulting from any disease or injury, including stroke, spinal cord injury, Alzheimer's disease, multiple sclerosis, amylolaterosclerosis (ALS), and multiple systems atrophy (MSA). [0136] Autoimmune diseases or infectious diseases that affect the basal ganglia may result in disorders of the basal ganglia including ADHD, OCD, tics, Tourette's disease, Sydenham chorea. In addition, any insult to the brain can potentially damage the basal ganglia including strokes, metabolic abnormalities, liver disease, multiple sclerosis, infections, tumors, drug overdoses or side effects, and head trauma, hi certain embodiments, PDElO inhibitors may be used to stop disease progression or restore damaged circuits in the brain by a combination of effects including increased synaptic plasticity, neurogenesis, anti-inflammatory, nerve cell regeneration and decreased apoptosis
[0137] The growth of some cancer cells is inhibited by cAMP and cGMP. Upon transformation, cells may become cancerous by expressing PDElO and reducing the amount of cAMP or cGMP within cells. In these types of cancer cells, inhibition of PDElO activity will inhibit cell growth by raising cAMP (US20040138249, Pyrrolo (2.1a)dihydroisoquinolines and their use as phosphodiesterase 10a inhibitors). In some cases, PDElO may be expressed in the transformed, cancerous cell but not in the parent cell line. In transformed renal carcinoma cells, PDElO is expressed and PDElO inhibitors reduce the growth rate of the cells in culture. Similarly, breast cancer cells are inhibited by administration of PDElO inhibitors. Many other types of cancer cells may also be sensitive to growth arrest by inhibition of PDElO. Therefore, chemical entities described herein may be used to stop the growth of cancer cells that express PDElO. [0138] The chemical entities described herein are also suitable for use in the treatment of diabetes and related disorders such as obesity, by focusing on regulation of the cAMP signaling system. By inhibiting PDE-IOA activity, intracellular levels of cAMP and increased, thereby increasing the release of insulin-containing secretory granules and, therefore, increasing insulin secretion. See, e.g., WO 2005/012485. [0139] Thus, in certain embodiments, there is provided a method of treating diabetes and related disorders comprising administering to a patient, such as a mammal, such as a human, a therapeutically effective amount of at least one chemical entity described herein. In certain embodiments, there is provided a method of treating type 1 diabetes, type 2 diabetes, Syndrome X, impaired glucose tolerance, impaired fasting glucose, gestational diabetes, maturity-onset diabetes of the young (MODY), latent autoimmune diabetes adult (LADA), associated diabetic dyslipidemia, hyperglycemia, hyperinsulinemia, dyslipidemia, hypertriglyceridemia, obesity and insulin resistance, comprising administering to a patient, such as a mammal, such as a human, a therapeutically effective amount of at least one chemical entity described herein. [0140] The chemical entities described herein may also be administered in combination with other known therapies for the treatment of diabetes, including, but not limited to, PPAR ligands (e.g. agonists, antagonists, such as Rosiglitazone, Troglitazone and Pioglitazone), insulin secretagogues (for example, sulfonylurea drugs (such as Glyburide, Glimepiride, Chlorpropamide, Tolbutamide, and Glipizide) and non-sulfonyl secretagogues), D-glucosidase inhibitors (such as Acarbose, Miglitol, and Voglibose), insulin sensitizers (such as the PPAR-D agonists, e.g., the glitazones; biguanides, PTP- IB inhibitors, DPP-IV inhibitors and 1 lbeta-HSD inhibitors), hepatic glucose output lowering compounds (such as glucagon antagonists and metaformin, such as Glucophage and Glucophage XR), insulin and insulin derivatives (both long and short acting forms and formulations of insulin), and anti-obesity drugs (such as D -3 agonists, CB-I agonists, neuropeptide Y5 inhibitors, Ciliary Neurotrophic Factor and derivatives (e.g., Axokine), appetite suppressants (e.g., Sibutramine), and lipase inhibitors (e.g., Orlistat)). [0141] The dosages of the chemical entities described hereindepend upon a variety of factors including the particular syndrome to be treated, the severity of the symptoms, the route of administration, the frequency of the dosage interval, the particular compound utilized, the efficacy, toxicology profile, pharmacokinetic profile of the compound, and the presence of any deleterious side-effects, among other considerations. [0142] The chemical entities described herein are typically administered at dosage levels and in a mammal customary for PDElO inhibitors such as those known compounds mentioned above. For example, the compounds can be administered, in single or multiple doses, by oral administration at a dosage level of generally 0.001-100 mg/kg/day, for example, 0.01-100 mg/kg/day, such as 0.1-70 mg/kg/day, for example, 0.5-10 mg/kg/day. Unit dosage forms can contain generally 0.01-1000 mg of active compound, for example, 0.1-50 mg of active compound. For intravenous administration, the compounds can be administered, in single or multiple dosages, at a dosage level of, for example, 0.001-50 mg/kg/day, such as 0.001-10 mg/kg/day, for example, 0.01-1 mg/kg/day. Unit dosage forms can contain, for example, 0.1-10 mg of active compound. [0143] In carrying out the procedures described herein, it is of course to be understood that reference to particular buffers, media, reagents, cells, culture conditions and the like are not intended to be limiting, but are to be read so as to include all related materials that one of ordinary skill in the art would recognize as being of interest or value in the particular context in which that discussion is presented. For example, it is often possible to substitute one buffer system or culture medium for another and still achieve similar, if not identical, results. Those of skill in the art will have sufficient knowledge of such systems and methodologies so as to be able, without undue experimentation, to make such substitutions as will optimally serve their purposes in using the methods and procedures disclosed herein.
[0144] The present invention will now be further described by way of the following non-limiting examples, hi applying the disclosure of these examples, it should be kept clearly in mind that other and different embodiments of the methods disclosed according to the present invention will no doubt suggest themselves to those of skill in the relevant art. [0145] In the foregoing and in the following examples, all temperatures are set forth uncorrected in degrees Celsius; and, unless otherwise indicated, all parts and percentages are by weight.
EXAMPLES
[0146] In order that the invention described herein may be more readily understood, the following examples are set forth. It should be understood that these examples are for illustrative purposes only and are not to be construed as limiting this invention in any manner.
[0147] All NMR spectra were recorded at 300 MHz on a Bruker Instruments
NMR unless otherwise stated. Coupling constants (J) are in Hertz (Hz) and peaks are listed relative to TMS (δ 0.00 ppm). Microwave reactions were performed using a Personal Chemistry Optimizer™ microwave reactor in 10 mL Personal Chemistry microwave reactor vials. All reactions were performed with the fixed hold time ON unless otherwise stated. Sulfonic acid ion exchange resins (SCX) were purchased from Varian Technologies. Analytical HPLC was performed on 4.6 mm x 100 mm Xterra RPi8 3.5 μ columns using (i) a gradient of 20/80 to 80/20 water (0.1% formic acid)/acetonitrile (0.1% formic acid) over 6 min (Method A), (ii) a gradient of 20/80 to 80/20 water (0.1% formic acid)/acetonitrile (0.1% formic acid) over 8 min (Method B), (iii) a gradient of 40/80 to 80/40 water (0.1% formic acid)/acetonitrile (0.1% formic acid) over 6 min (Method C), (iv) a gradient of 40/80 to 80/40 water (0.1% formic acid)/acetonitrile (0.1 % formic acid) over 8 min (Method D), or (v) an isocratic of 80/20 acetonitrile (0.1% formic acid)/water (0.1% formic acid) over 8 min (Method E). Preparative HPLC was performed on 30 mm x 100 mm Xtera Prep RP18 5 μ columns using an 8 min gradient of 95/5 to 20/80 water (0.1% formic acid)/acetonitrile (0.1% formic acid).
Example 1
4-(l-Acetyl-piperidin-4-yl)-2-aminothiophene-3-carboxylic acid ethyl ester [0148] [See, e.g., Guetschow, Michael and Neumann, UIf, J. Med. Chem, 41, 10,
1729-1740, 1998].
Figure imgf000109_0001
[0149] A mixture of l-(4-acetylpiperidino)ethan-l-one (1.020 g, 6.028 mmol) , sulfur (0.193 g, 6.03 mmol), cyanoacetic acid ethyl ester (0.682 g, 6.03 mol) and ethanol (2.00 mL) was treated with morpholine (0.525 g, 0.00603 mol) dropwise at 45 °C. After 12 hours, the reaction mixture was diluted with ethyl acetate (60.0 mL), filtered and washed with of 5% aqueous sodium bicarbonate (2 x 40 mL). The organic layer was concentrated and purified by chromatography over silica gel using 2% methanol, 0.02% ammonia, 49% ethyl acetate, and 49% hexanes to give 700 mg (39%) of 4-(l -acetyl - piperidin-4-yl)-2-aminothiophene-3-carboxylic acid ethyl ester as a light brown solid. MS [M+H] 297.1, 1H NMR (CDCl3) δ (ppm) 6.10 (b, 2H), 5.85 (s, IH), 4.85 (d, J = 13.5 Hz, IH), 4.32 (q, J = 7.2 Hz, 2H), 3.88 (m, IH), 3.31 (m, IH), 3.12 (m, IH), 2.61 (m, IH), 2.12 (s, 3H), 2.00 (m, 2H), 1.62-1.35 (m, 5H).
[0150] The following compounds were synthesized in a similar manner using different starting materials:
4-(Pyridine-4-yl)-2-aminothiophene-3-carboxylic acid ethyl ester, 4-(Pyridine-3-yl)-2-aminothiophene-3-carboxylic acid ethyl ester, 4-(Pyridine-2-yl)-2-aminothiophene-3-carboxylic acid ethyl ester, 4-(Thiazol-2-yl)-2-aminothiophene-3-carboxylic acid ethyl ester, 4-(2-Furyl)-2-aminothiophene-3-carboxylic acid ethyl ester.
Example 2
4-Hydroxy-5-(2-furyl)thieno[2,3-d]pyrimidine
[0151] [See, e.g., Perrissin, Monique; Favre, Marylene; Luu-Duc, Cuong et al;
Eur. J. Med. Chem.Chim. Ther., 19, 5, 420-424, 1984].
Figure imgf000110_0001
[0152] A mixture of ethyl 2-amino-4-(2-furyl)thiophene-3-carboxylate (1.00 g,
4.21 mmol) and formamide (10.0 g, 0.2 mol) was warmed by microwave at 200 °C for 10 minutes. The mixture was concentrated in vacuo at 90 0C, diluted with 40 mL of 10% methanol/dichloromethane, and filtered through a 40 gram silica gel column using 5% methanol/dichloromethane to wash the material through the column. The filtrate was again concentrated and the remaining residue was purified by column chromatography over silica gel using a gradient elution from 5 to 10% methanol in dichloromethane to give 0.482 g (52.4%) of 4-hydroxy-5-(2-furyl)thieno[2,3-d]pyrimidine as a brown solid. MS [M+H] 219.1, 1H NMR (10% CD3OD/CDC13) δ (ppm) 7.90 (s, IH), 7.50 (s, IH), 7.45 (d, J = 3.3 Hz, IH), 7.40 (d, J = 1.8 Hz, IH), 5.43 (dd, J = 3.3, 1.8 Hz, IH). [0153] The following compounds were synthesized in a similar manner using different starting materials:
4-Hydroxy-5-(pyridin-4-yl)thieno[2,3-d]pyrimidine,
4-Hydroxy-5-(pyridin-3-yl)thieno[2,3-d]pyrimidine,
4-Hydroxy-5-(pyridin-2-yl)thieno[2,3-d]pyrimidine,
4-Hydroxy-5-(thiazol-2-yl)thieno[2,3-d]pyrimidine,
4-Hydroxy-5-(l-acetylpiperidin-4-yl)thieno[2,3-d]pyrimidine.
Example 3 4-Chloro-5-(thiazol-2-yl)thieno[2,3-d]pyrimidine
Figure imgf000111_0001
[0154] A mixture of thionyl chloride (15 ml, 200 mmol), 4-hydroxy-5-(thiazol-2- yl)thieno[2,3-d]pyrimidine (1.44 g, 12 mmol) and N,N-dimethylformamide (3 mL) was heated at 73 0C for 3 hours and then carefully poured into 200 mL of ice/water. The pH was adjusted to between 7 and 8 with sodium bicarbonate and then the product was extracted with ethyl acetate (2 x 450 mL). The organic fractions were combined, dried (MgSO4) and concentrated. The resulting residue was purified by column chromatography over silica gel using a gradient elution from 10 to 20% ethyl acetate in hexanes to give 0.825 g (53%) of 4-chloro-5-(thiazol-2-yl)thieno[2,3-d]pyrimidine as a brown solid. MS [M+H] 254.0, 1H NMR (400 MHz, 10% CD3OD/CDC13) δ (ppm) 8.92 (s, IH), 8.00 (d, J = 3.2 Hz, IH), 7.85 (s, IH), 7.55 (d, J = 3.2 Hz, IH). [0155] The following compounds were synthesized in a similar manner using different starting materials:
4-Chloro-5-(pyridin-4-yl)thieno[2,3-d]pyrimidine, 4-Chloro-5-(pyridin-3-yl)thieno[2,3-d]pyrimidine, 4-Chloro-5-(pyridin-2-yl)thieno[2,3-d]pyrimidine, 4-Chloro-5-(2-furyl)thieno[2,3-d]pyrimidine.
Example 4
2) N-[3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}- propoxy)phenyl] acetamide.
Figure imgf000112_0001
[0156] A mixture of 4-chloro-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine (100 mg, 0.378 mmol), N-[3-(3-amino-propoxy)-phenyl]-acetamide (94 mg, 0.45 mmol), diisopropylethylamine (147 mg, 1.14 mmol) and N,N-dimethylformamide (3.0 mL) was warmed to 120 0C for 20 minutes by microwave irradiation. The solvent was then concentrated in vacuo and the residue was dissolved in ethyl acetate (50 mL) and washed with of aqueous sodium bicarbonate (2 x 30 mL). The organic layer was concentrated in vacuo and purified by column chromatography over silica gel using 1% methanol in dichloromethane as eluant to give 125 mg (73%) of N-[3-(3-{[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)phenyl]acetamide as a white solid. MS [M+H] 437.1, 1H NMR (CDCl3) δ (ppm) 8.50 (s, IH), 7.41-7.36 (m, 2H), 7.25 (s, IH), 7.21 (t, J = 8.1 Hz, IH), 7.07-6.95 (m, 3H), 6.94 (d, J = 8.1 Hz, IH), 6.55 (d, J = 8.4 Hz, IH), 5.01 (b, IH), 3.89 (t, J = 6.0 Hz, 2H), 3.64 (m, 2H), 2.19 (s, 3H), 1.98 (m, 2H).
[0157] The following compounds were synthesized in a similar manner using different starting materials:
9) 3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- benzonitrile, MS[M+H] 386.2
10) Methyl 3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)- methyl]benzoate, MS[M+H] 419.2 11 ) N-(5-phenylthieno[2,3-d]pyrimidin-4-yl)-N'-[3-(trifluoromethoxy)benzyl]ethane- 1,2-diamine, MS[M+H] 445.2
99) N-(3-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide, MS[M+H] 420.1
100) N-[3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]ρyrimidin-4-yl]amino}propoxy)- phenyl]acetamide, MS[M+H] 426.0
101) N-(3-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide, MS[M+H] 420.1
102) N-[4-(3 - { [5-( 1 ,3 -thiazol-2-yl)thieno[2,3 -d]pyrimidin-4-yl] amino } propoxy)- phenyljacetamide, MS[M+H] 426.0
103) N-(4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide, MS[M+H] 420.0
104) N-[4-(3- {[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyljacetamide, MS[M+H] 443.1
Example 5 3) N-(3-Nitrobenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2-diamine
Figure imgf000113_0001
[0158] A mixture of N-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l ,2-diamine
(Maybridge) (30 mg, 0.11 mmol), 3-nitrobenzaldehyde (18 mg, 0.12 mmol), acetic acid (6 mg, 0.10 mmol) and ethanol (3.0 mL) was stirred at room temperature for 30 minutes, followed by the addition of 7.6 mg (0.20 mmol) of sodium borohydride (7.6 mg, 0.20 mmol). The reaction was stirred for an additional 30 minutes and then water (0.2 mL) and ethyl acetate (20 mL) were added. The mixture was washed with aqueous sodium bicarbonate solution (2 x 20 mL) and the organic layer was separated, concentrated, and purified by column chromatography over silica gel using a gradient elution from 2.5% to 5% methanol in dichloromethane as eluant to give 34.2 mg (77%) N-(3-nitrobenzyl)-N'- (5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2-diamine as a colorless gum. MS [M+H] 406.2, 1H NMR (CDCl3) δ (ppm) 8.49 (s, IH), 8.11 (d, J = 6.6 Hz, IH), 8.06 (s, IH), 7.50-7.32 (m, 7H), 7.08 (s, IH), 5.48 (b, IH), 3.75 (s, 2H), 3.55 (m, 2H), 2.75 (m, 2H). [0159] The following compounds were synthesized in a similar manner using different starting materials:
4) N-(3,4-dimethoxybenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2- diamine, MS[M+H] 421.2
5) N-(2,l,3-benzothiadiazol-5-ylmethyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4- yl)ethane-l,2-diamine, MS[M+H] 419.1
6) N-{3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- phenyl}acetamide, MS {M+H] 418.2
7) N-{4-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- ρhenyl}acetamide, MS[M+H] 418.2
Example 6
N-(3 -formylphenyl)acetamide
Figure imgf000114_0001
[0160] A mixture of N-(3-hydroxymethylphenyl)acetamide (165 mg, 1.0 mmol), celite (165 mg), pyridinium chlorochromate (645 mg, 3.0 mmol), N ,N- dimethylformamide (1.0 mL) and dichloromethane (25 mL) was stirred at room temperature for 24 h. The reaction mixture was filtered and concentrated in vacuo. The resulting residue was dissolved in dichloromethane (40 mL) and washed with aqueous sodium bicarbonate solution (2 x 30 mL). The organic layer was concentrated and the product purified by chromatography over silica gel using ethyl acetate/hexanes (1 : 1) as eluant to give 130 mg (81%) of N-(3-formylphenyl)acetamide as a brown solid. MS [M+H] 164.2, 1H NMR (CDCl3) δ (ppm) 9.96 (s, IH), 8.22 (b, IH), 8.03 (s, IH), 7.89 (d, J = 7.8 Hz, IH), 7.61 (d, J - 6.6 Hz, IH), 7.47 (t, J = 7.8 Hz, IH), 2.22 (s, IH). Example 7 19) 3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}-propoxy)aniline
Figure imgf000115_0001
[0161] 5-(4-Fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3- d]pyrimidine (250 mg, 0.588 mmol) was dissolved in tetrahydrofuran (15 mL) and added (under an atmosphere of argon) to a suspension of 25 mg of 10% palladium/carbon in methanol (30 mL) and acetic acid (5 mL). The mixture was then shaken under 40 psi of hydrogen overnight. The palladium/carbon was removed by filtration and the filtrate was concentrated in vacuo. The residue was dissolved in ethyl acetate (50 mL) and washed with aqueous sodium bicarbonate solution (2 x 30 mL). The organic layer was separated, concentrated, and purified by column chromatography over silica gel using 33% ethyl acetate in hexanes as eluant to give 180 mg (76%) of 3-(3-{[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)aniline as a colorless gum. MS [M+H] 396.1, 1H NMR (CDCl3) δ (ppm) 8.65 (s, IH), 7.45-7.40 (m, 2H), 7.21 (s, IH), 7.09-7.00 (m, 3H), 6.30 (d, J= 2.1 Hz, IH), 6.20 (d, J = 2.8 Hz, IH), 6.11 (s, IH), 4.58 (t, J = 6.0 Hz, 2H), 3.69 (t, J = 6.0 Hz, 3H), 3.68 (b, 2H), 2.05 (m, 2H). [0162] The following compounds were synthesized in a similar manner using different starting materials: 4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)aniline
Example 8
21) N-[3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy) phenyl] cyclopropanecarboxamide.
Figure imgf000116_0001
DIPEA/DCM
Figure imgf000116_0002
Figure imgf000116_0003
[0163] A mixture of 3-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)aniline (10 mg, 0.025 mmol), diisopropylethylamine (13 mg, 0.10 mmol), and dichloromethane (2 mL) was treated with cyclopropanecarbonyl chloride (30 mg, 0.028 mmol) and stirred at room temperature for 6 hours. The solvent was removed in vacuo and the remaining residue was dissolved in ethyl acetate (25 mL), washed with aqueous sodium bicarbonate solution (2 x 20 mL), and the organic fraction was separated and concentrated. The product was purified by column chromatography over silica gel using 1% methanol and 0.01% ammonia in 1 :1 ethyl acetate/hexanes to give 12 mg (quantitative yield) of N-[3-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]cyclopropanecarboxamide as a yellow gum. MS [M+H] 464.0, 1H NMR (CDCl3) δ (ppm) 8.65 (s, IH), 7.48 (s, IH), 7.50-7.36 (m, 2H), 7.21 (s, IH), 7.16 (d, J = 8.1 Hz, IH), 7.10-7.00 (m, 2H), 6.95 (d, J = 7.5 Hz, IH), 6.52 (d, J = 8.1 Hz, IH), 4.58 (t, J = 6.0 Hz, 2H), 3.74 (t, J = 6.0 Hz, 2H), 2.05 (m, 2H), 1.67 (m, IH), 1.20- 0.80 (m, 4H).
Example 9
27) N-(2-Amino-2-oxoethyl)-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzamide
Figure imgf000117_0001
[0164] 1,3-Diisopropylcarbodiimide (13.0 mg, 0.10 mmol) was added to a stirred mixture of 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoic acid (21 mg, 0.050 mmol), 2-amino-acetamide hydrochloride (11 mg, 0.10 mmol), 1-hydroxybenzotriazole (6.7 mg, 0.05 mmol), diisopropylethylamine (40 mg, 0.30 mmol) and 4-dimethylaminopyridine (13 mg, 0.10 mmol) in N,N-dimethylformamide (1.0 mL). The mixture was stirred at room temperature for 6 hours and then concentrated in vacuo. The resultant residue was dissolved in ethyl acetate (30 mL), washed with aqueous sodium bicarbonate solution (2 x 25 mL) and the organic layer was separated and concentrated. The product was purified by column chromatography over silica gel using 5% methanol in 1 :1 of ethyl acetate/hexanes to give 9.6 mg (40%) of N-(2-amino-2- oxoethyl)-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzamide as a white solid. MS [M+H] 481.1, 1H NMR (CDCl3) δ (ppm) 8.68 (s, IH), 7.60-7.24 (m, 4H), 7.21 (s, IH), 7.18-6.95 (m, 3H), 6.90 (d, J = 7.8 Hz, IH), 6.31(b, IH), 5.62 (b, IH), 4.59 (t, J = 6.0 Hz, 2H), 4.19, (d, J = 5.1 Hz, 2H), 3.74 (t, J = 6.0 Hz, 2H), 2.10 (m, 2H).
[0165] The following compounds were synthesized in a similar manner using different starting materials: 8) 3-(Acetylamino)-N-{2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}- benzamide. MS[M+H] 432.2
22) 5-(4-Fluorophenyl)-4-(3-{3-[(4-methylpiperazin-l-yl)carbonyl]phenoxy}- propoxy)thieno[2,3-d]pyrimidine. MS[M+H] 507.1
23) 5-(4-Fluorophenyl)-4-{3-[3-(moφholin-4-ylcarbonyl)phenoxy]propoxy}- thieno[2,3-d]pyrimidine. MS[M+H] 494.1
24) N-Ethyl-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide. MS[M+H] 452.1 25) 3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N,N- dimethylbenzamide MS[M+H] 452.1
26) N-[2-(Dimethylamino)ethyl]-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzamide. MS[M+H] 495.1
28) 3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N'- formylbenzohydrazide. MS[M+H] 467.0
Example 10
35) 3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy) benzohydrazide
Figure imgf000118_0001
(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoic acid (43 mg, 0.10 mmol) in tetrahydrofuran (2.0 mL) with stirring at 70 ° C. Stirring continued for one hour at 70 °C and then the mixture was cooled to 25 0C and added to a solution of hydrazine (32 mg, 1.0 mmol) in tetrahydrofiiran (2.0 mL). The reaction was stirred at room temperature for one hour and the solvent was then removed in vacuo. The residue was dissolved in ethyl acetate (30 mL), washed with of aqueous sodium bicarbonate solution (20 mL), concentrated, and purified by column chromatography over silica gel using 2% methanol in ethyl acetate as eluant to give 22 mg (50%) of 3-(3-{[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzohydrazide as a white solid. MS [M+H] 439.2, 1H NMR (CDCl3) δ (ppm) 8.65 (s, IH), 7.50-7.35 (m, 2H), 7.32-7.21 (m, 3H), 7.18 (s, IH), 7.10-6.97 (m, 2H), 6.94 (d, J = 2.7 Hz, IH), 4.59 (t, J = 6.9 Hz, 2H), 4.11 (s, 2H), 3.74 (t, J = 6.0 Hz, 2H), 2.09 (m, 2H).
[0167] The following compound was synthesized in a similar manner using different starting materials: 36) 3 -(3 - { [5 -(4-fluorophenyl)thieno [2 ,3 -d] pyrimidin-4-yl] oxy } propoxy)-N- hydroxybenzamide. MS[M+H] 440.2
Example 11
3 -Hydroxy-N-methylbenzamide
HO COOCH,
Figure imgf000119_0001
[0168] A solution of 3-hydroxybenzoic acid methyl ester (2.00 g, 0.0131 mol), methylamine (0.06 mol) and methanol (30 mL) was heated to 155 °C for 12 hours in a sealed tube. The solvent was removed in vacuo and the product was purified by column chromatography over silica gel using a gradient elution from 5 to 8% methanol in dichloromethane to give 1.20 g (60.4%) of 3-hydroxy-N-methylbenzamide as a yellow solid. MS [M+H] 152.2, 1H NMR (CDCl3) δ (ppm) 7.55 (s, IH), 7.30 (d, J = 7.8 Hz, IH), 7.18 (d, J = 7.5 Hz, IH), 7.01 (m, IH), 6.65 (b, IH), 6.20 (b, IH), 3.03 (d, J = 4.8 Hz, 3H).
[0169] The following compounds were synthesized in a similar manner using different starting materials:
3-hydroxybenzamide. MS[M+H] 138.1
5-hydroxynicotinamide. MS[M+H] 139.1
5-hydroxy-N-methylnicotinarnide. MS[M+H] 153.2
5-hydroxy-N-methylpyridine-2-carboxarnide. MS[M+S] 153.1
5-hydroxypyridine-2-carboxamide. MS[M+S] 139.1
Example 12a
54) 5-{3-[5-(4-Fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid
Figure imgf000119_0002
[0170] To a solution of 5- {3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4- yloxy]-propoxy}-nicotinic acid methyl ester (100 mg) in dioxane (10 mL) was added 0.80 M lithium hydroxide in water (2.0 mL). The reaction was stirred at ambient temperature for 16 hours, then acidified with IM hydrochloric acid to pH 3, and the solvent removed under reduced pressure. The residue was dissolved in 20% methanol/dichloromethane (40 mL), filtered, and the filtrate was concentrated. The product was purified by column chromatography over silica gel using 10% methanol in dichloromethane as eluant to give 90 mg (93%) of 5-{3-[5-(4-fluorophenyl)-thieno[2,3- d]pyrimidin-4-yloxy]-propoxy} -nicotinic acid as a white solid. MS [M+H] 425.9, 1H NMR (CD3OD) δ (ppm) 8.91 (s, IH), 8.63 (s, IH), 8.62 (s, IH), 8.27 (s, IH), 7.60-7.45 (m, 3H), 7.10 (t, J = 8.7 Hz, 2H), 4.67 (t, J = 9.0 Hz, 2H), 3.94 (t, J = 9.3 Hz, 2H), 2.22 (t, J = 9.0 Hz, 2H).
[0171] The following compound was synthesized in a similar manner using different starting materials:
20) 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoic acid. MS [M+H] 425.0
Example 12b
88) 5- {4-[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinic acid hydroformate
Figure imgf000120_0001
[0172] A mixture of methyl-5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]butoxy}nicotinate (0.010 g, 0.023 mmol) and potassium hydroxide (0.0112 g, 0.200 mmol) in methanol (0.5 mL) was stirred at room temperature for 12 h. Acetic acid (200 mg, 3.33 mmol) was then added, and the reaction solvent was removed by evaporation. The product was purified by column chromatography over silica gel using 5% methanol in dichloromethane as eluant to afford 8 g (83%) 5-{4-[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl]butoxy} nicotinic acid hydroformate as white solid. MS [M+H] = 424.1, LC/MS (EI) tR 3.89 min (Method D), 1H NMR (10% MeOD/CDCl3) δ (ppm) 8.94 (s, IH), 8.73 (s, IH), 8.28 (s, IH), 7.68 (s, IH), 7.40-7.30 (m, 3H), 7.10 (t, J = 8.4 Hz, 2H), 3.84 (t, J = 6.3 Hz, 2H), 2.68 (t, J = 7.2 Hz, 2H), 1.75-1.59 (m, 2H), 1.59-1.45 (m, 2H)
Example 13 5-(3-Hydroxypropoxy)-N-methylpyridine-2-carboxamide
Figure imgf000121_0001
[0173] To a mixture of 5-hydroxy-N-methylpyridine-2-carboxamide (400 mg, 3.0 mmol), 1,3 -propanediol (230 mg, 3.0 mmol), triphenylphosphine (786 mg, 3.0 mmol) and N,N-dimethylformamide (4.5 ml, 60 mmol) was added diisopropyl azodicarboxylate (606 mg, 3.0 mmol) while stirring at ambient temperature. After 18 hours, the solvent was removed under reduced pressure and the resulting residue was taken up in ethyl acetate (40 mL) and filtered. The filtrate was concentrated and purified by column chromatography over silica gel using methanol/ethyl acetate (3-4%) to give 488 mg (80%) of 5-(3-hydroxypropoxy)-N-methylpyridine-2-carboxamide as a colorless gum. MS [M+H] 211.2, 1H NMR (CDCl3) δ (ppm) 8.19 (d, J = 3.0 Hz,lH), 8.13 (d, J = 8.7 Hz, IH), 7.83 (b, IH), 7.28 (m, IH), 4.21 (t, J - 6.3 Hz, 2H), 3.88 (m, 2H), 3.02 (d, J = 5.1 Hz), 2.08 (m, 2H).
[0174] The following compounds were synthesized in a similar manner using different starting materials:
N-[3-(3-hydroxy-propoxy)-phenyl]-acetamide. MS[M+H] 210.3 5-(3-hydroxypropoxy)pyridine-2-carboxamide. MS[M+H] 197.2
15) N-[4-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide. MS[M+H] 438.2
16) 5-(4-fluorophenyl)-4-{3-[4-(lH-l,2,4-triazol-l-yl)phenoxy]propoxy}thieno[2,3- d]pyrimidine. MS[M+H] 448.1
17) 4-[3-(4-fluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine. MS[M+H] 399.1 18) methyl 3-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzoate. MS[M+H] 439.1 12) 5-(4-fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3-d]pyrimidine.
MS[M+H] 426.0
29) 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- methylbenzamide. MS[M+H] 438.2
30) 3 -(3 - { [5-(4-fluorophenyl)thieno [2,3 -d]pyrimidin-4-yl]oxy} propoxy)benzamide. MS[M+H] 424.2
31) 3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)-N- methylbenzamide. MS[M+H] 424.2
32) N-[3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)phenyl]- urea. MS[M+H] 425.2
33) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- urea. MS[M+H] 439.2
34) 3-(2- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy} ethoxy)benzamide. MS[M+H] 410.2
50) 4-{3-[5-(4-fluorophenyl)-thieno[2,3d]pyrimidin-4-yloxy]-propoxy}-benzamide. MS[M+H] 424.0
37) 4-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)benzamide.
MS[M+H] 410.1 53) 2-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzamide.
MS[M+H] 424.1
51) 5- {3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid methyl ester. MS[M+H] 440.0
Example 14
61) N-Methyl-5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy} pyridine-2-carboxamide
Figure imgf000123_0001
[0175] 5-(3-Hydroxypropoxy)-N-methylpyridine-2-carboxamide (52 mg, 0.25 mmol) in N,N-dimethylacetamide (2.0 mL, 22 mmol) was treated with sodium hydride (11 mg, 60%, 0.27 mmol) at ambient temperature. After 1.5 hours, the mixture was added to a solution of 4-chloro-5-pyridin-4-ylthieno[2,3-d]pyrimidine (62 mg, 0.025 mmol) in N,N-dimethylacetamide (1.0 mL) and the resulting mixture was stirred at room temperature for 12 hours. The reaction mixture was then quenched by the addition of acetic acid (0.12 mL, 2.0 mmol) and the solvent was removed under reduced pressure. The residue was dissolved in ethyl acetate (50 mL), washed with 5% aqueous sodium bicarbonate (2 x 30 mL) and the organic layer was concentrated. The product was purified by silica gel chromatography using 3 to 5% methanol in ethyl acetate to give 72 mg (68%) of N-methyl-5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4- yl)oxy]propoxy}pyridine-2-carboxamide as a white solid. MS [M+H] 422.0, 1H NMR (CDCl3) δ (ppm) 8.74 (s, IH), 8.66 (s, IH), 8.54 (d, J = 3.9 Hz, IH), 8. 11 (d, J = 8.4 Hz, IH), 8.05 (d, J = 2.7 Hz, IH), 7.90-7.73 (m, 2H), 7.33 (s, IH), 7.28 (dd, J = 7.8, 4.8 Hz, 1 H), 7.11 (dd, J - 8.7, 2.7 Hz, IH), 4.62 (t, J = 6.0 Hz, 2H), 3.76 (t, J = 6.0 Hz, 2H), 3.01 (d, J = 4.8 Hz, 3H), 2.13 (t, J = 6.0 Hz, 2H).
[0176] The following compounds were synthesized in a similar manner using different starting materials:
3-[5-(4-flurophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propan-l-ol.
MS[M+H] 305.2
5-(4-fluorophenyl)-4-isopropoxythieno[2,3-d]pyrimidine.
MS[M+H] 289.2 105) 5-(4-fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3-d]pyrimidine.
MS[M+H] 426.1 14) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide. MS [M+H] 438.4 2- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy} ethanol. MS[M+H] 291.2
52) N-{3-[3-(5-fiiran-2-yl-thieno[2,3-d]pyrimidin-4-yloxy)-propoxy]-phenyl}- acetamide. MS[M+H] 410.0
N-{3-[3-(4-oxo-5-furan-2yl-4-oxo-4H-thieno[2,3-d]pyrimidin-l-yl)propoxy]- phenyl}acetamide. MS[M+H] 410.1
N-{3-[3-(4-oxo-5-phenylthieno[2,3-d]pyrimidin-l(4H)-yl)propoxy]phenyl}- acetamide. MS[M+H] 420.0
Benzyl 4-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethyl)- piperazine-1-carboxylate. MS[M+H] 493.1
57) 5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}ρyridine-2- carboxamide. MS [M+H] 408.1
58) 5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide. MS [M+H] 425.1
59) 5-(3- {[5-(l ,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide. MS[M+H] 414.1
60) 5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide. MS[M+H] 408.0
62) 5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- methylpyridine-2-carboxamide. MS[M+H] 439.1
63) N-methyl-5-(3- {[5-(l ,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- pyridine-2-carboxamide. MS[M+H] 428.0
Diethyl [5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]malonate. MS[M+H] 389.1
64) N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- pyridine-2-carboxamide. MS[M+H] 422.1
106) N-methyl-4- {3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- benzamide. MS[M+H] 421.1
107) N-methyl-5-(3- {[5-(l ,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- nicotinamide. MS[M+H] 428.0 108) N-methyl-5- {3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- nicotinamide. MS[M+H] 422.1
109) N-methyl-3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide. MS[M+H] 427.0
110) N-[4-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide. MS[M+H] 427.0
111) N-(4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}phenyl)- acetamide. MS[M+H] 421.0
65) N-methyl-3-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy} benzamide, MS [M+H] = 421.1, LC/MS (EI) tR 5.42 min (Method B)
71) 5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide hydrofoπnate, MS [M+H] = 408.1, LC/MS (EI) tR 4.14 min (Method B)
72) 5-(4-fluorophenyl)-4-(3-{[l-methyl-5-(trifluoromethyl)-lH-pyrazol-3-yl]oxy} proρoxy)thieno[2,3-d]pyrimidine5 MS [M+H] = 453.1, LC/MS (EI) tR 4.32 min (Method E)
73) 4-[3-(4-chlorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, MS [M+H] = 415.1, LC/MS (EI) tR 5.65 min (Method E)
74) 4-[3-(3,5-difluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, MS [M+H] = 417.1, LC/MS (EI) tR 4.82 min (Method E)
75) 4-[3-(3,4-dimethoxyphenoxy)propoxy]-5-(4-fluorophenyl)thieno [2,3-d]pyrimidine, MS [M+H] = 441.2, LC/MS (EI) tR 3.53 min (Method E)
76) 4-[3-(l,2-benzisothiazol-3-yloxy)propoxy]-5-(4-fluorophenyl)thieno [2,3-d]pyrimidine, MS [M+H] = 438.1, LC/MS (EI) tR 5.89 min (Method E)
77) 5-(4-fluorophenyl)-4-(3-phenoxypropoxy)thieno[2,3-d]pyrimidine, MS [M+H] = 356.1, LC/MS (EI) tR 5.24 min (Method E)
78) 5-(4-fluorophenyl)-4-[3-(3-methoxyphenoxy)propoxy]thieno[2,3-d]pyrimidine, MS [M+H] = 411.1, LC/MS (EI) tR 4.25 min (Method E)
79) 5-(4-fluorophenyl)-4-[3-(3-methylphenoxy)propoxy]thieno[2,3-d]pyrimidine, MS [M+H] = 395.1, LC/MS (EI) tR 5.36 min (Method E)
80) 5-(4-fluorophenyl)-4-{3-[3-(trifluoromethoxy)phenoxy]propoxy}thieno [2,3-d]pyrimidine, MS [M+H] = 465.1, LC/MS (EI) tR 5.61 min (Method E) 81 ) 4-[3-(3-ethylphenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, MS
[M+H] = 409.1, LC/MS (EI) tR 6.06 min (Method E) 92) N-[3-(2,2-dimethyl-3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy} propoxy)phenyl]acetamide hydroformate, MS [M+H] = 455.1, LC/MS (EI) tR
6.13 min (Method D) 94) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}-2,2-dimethyl propoxy)phenyl]acetamide hydroformate, MS [M+H] = 466.1, LC/MS (EI) tR
7.66 min (Method D).
Example 15
39) N-[3-(3-{[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]methanesulfonamide
Figure imgf000126_0001
[0177] A solution of 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)aniline (50 mg, 0.126 mmol) in dichloromethane (1.5 mL) was added to methanesulfonyl chloride (58 mg, 0.51 mmol) while stirring, followed by the addition of a solution of diisopropylethylamine (18 mg, 0.14 mmol) in dichloromethane (0.5 mL). Stirring was continued for 12 hours at room temperature and then the solvent was evaporated in vacuo. The residue was dissolved in ethyl acetate (30 mL), washed with sodium bicarbonate (2 x 25 mL) and the organic layer was isolated and concentrated. The product was purified by silica gel chromatography using 40% ethyl acetate in hexanes to give 23 mg (40%) of N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]methanesulfonamide as a yellow solid. MS [M+H] 474.7. 1H NMR (400 MHz, CDCl3) δ (ppm) 8.65 (s, IH), 7.42 (m, 2H), 7.23 (t, J = 68.0 Hz, IH), 7.22 (s, IH), 7.06-7.01 (m, 2H), 6.76 (dd, J = 8.0, 2.0 Hz, IH), 6.68 (t, J = 2.4 Hz, IH), 6.58 (dd, J = 8.0, 2.4 Hz, IH), 6.32 (b, IH), 4.58 (t, J = 6.0 Hz, 2H), 3.01 (s, 3H), 2.07 (m, 2H).
[0178] The following compounds were synthesized in a similar manner using different starting materials:
40) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- methanesulfonamide. MS[M+H] 474.7
41) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N-(methylsulfonyl)methanesulfonamide. MS{M+H] 552.8
42) N-(ethylsulfonyl)-N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]ethanesulfonamide. MS[M+H] 580.9
46) N- [3 -(3 - { [5-(4-fluorophenyl)thieno[2,3 -d]pyrimidin-4-yl]oxy } propoxy)phenyl] - ethanesulfonamide. MS[M+H] 488.2
43) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- ethanesulfonamide. MS[M-H] 486.7
Example 16
38) N-Ethyl-N'-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy} propoxy)phenyl] urea
1)triphosgene DCM, DIPEA
2) ethylamine
Figure imgf000127_0001
Figure imgf000127_0002
[0179] A mixture of 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)aniline (25 mg, 0.063 mmol) and diisopropylethylamine (9.0 mg, 0.069 mmol) in dichloromethane (1.5 mL) was added in three portions over a period of 30 minutes to a solution of triphosgene (7.0 mg, 0.023 mmol) in dichloromethane (0.5 mL) while stirring at ambient temperature. Stirring continued for 15 minutes followed by the addition of ethylamine (0.075 mL, 2M in tetrahydrofuran). The reaction was stirred for 2 hours, then diluted with dichloromethane (30 mL) and washed with sodium bicarbonate (2 x 20 mL). The organic layer was dried (Na2SO4), concentrated, and purified by column chromatography over silica gel using 40% ethyl acetate in hexanes as eluant to give 15 mg (51%) of N-ethyl-Nt-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]urea as a white solid. MS [M+H] 467.3. 1H NMR (400 MHz, CDCl3) δ (ppm) 8.64 (s, IH), 7.45-7.38 (m, 2H), 7.21 (s, IH), 7.18 (t, J = 8.0 Hz, IH), 7.06-7.01 (m, 2H), 6.82 (t, J - 2.4 Hz, IH), 6.79 (m, IH), 6.50 (m, IH), 4.58 (t, J = 6.0 Hz, 2H), 3.72 (t, J = 6.0 Hz, 2H), 3.30 (q, J = 7.2 Hz, 2H), 2.06 (m, 2H), 1.16 (t, J = 7.2 Hz, 3H).
[0180] The following compounds were synthesized in a similar manner using different starting materials:
44) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-methylurea. MS [M+H] 453.5
45) N-ethyl-N'-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]urea. MS [M+H] 467.9
47) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-methylurea. MS [M+H] 453.3
56) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]hydrazinecarboxamide. MS [M+H] 454.6 49) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-phenyl]-
N'-hydroxyurea. MS [M-H] 453.3
48) N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]hydrazinecarboxamide. MS [M-H] 452.2
Example 17
66) N-Methyl-5-(3-[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4- yl]aminopropoxy)pyridine-2-carboxamide
Figure imgf000128_0001
[0181] A mixture of 4-chloro-5-(l,3-thiazol-2-yl)thieno[2,3-d]ρyrimidine (0.050 g, 0.20 mmol), 5-(3-aminopropoxy)-N-methylpyridine-2-carboxamide trifluoroacetate (0.070 g, 0.22 mmol), potassium carbonate (0.272 g, 1.97 mmol) and N,N- dimethylacetamide (2.4 mL) was heated at 120 0C for 13 h. The solvent was evaporated in vacuo, and the residue was treated with 5% sodium bicarbonate (20 mL) and ethyl acetate (20 mL). The precipitate was filtered and washed with water and ethyl ether. The resulting white solid was purified by column chromatography over silica gel to give 70 mg (81%) N-methyl-5-(3-[5-(l,3-thiazol-2-yl)thieno[2,3-d]ρyrimidin-4- yl]aminopropoxy)pyridine-2-carboxamide. MS [M+H] = 427, LC/MS (EI) tR 3.54 min (Method D), 1H NMR (10% MeOD/CDCl3) δ (ppm) 10.85 (s, IH), 8.30 (s, IH), 8.12 (d, J = 2.4 Hz, IH), 8.00 (d, J = 8.7 Hz, IH), 7.94 (b, IH), 7.71 (s, IH), 7.50 (d, J = 3.6 Hz, IH), 7.28 (s, IH), 7.21 (dd, J = 8.7, 3.0 Hz, IH), 4.15 (t, J = 0.6 Hz, 2H), 3.80-3.74 (m, 2H), 2.94 (d, J = 0.6 Hz, 3H), 2.24-2.18 (m, 2H).
[0182] The following compounds were synthesized in a similar manner using different starting materials:
13) N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl]acetamide. MS[M+H] 437.2
67) 5-(3-{[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)-N- methylpyridine-2-carboxamide, MS [M+H] = 444, LC/MS (EI) tR 3.91 min (Method D)
68) N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy} pyridine-2-carboxamide, MS [M+H] = 421, LC/MS (EI) tR 5.2 min (Method B)
69) N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy} pyridine-2-carboxamide, MS [M+H] = 421, LC/MS (EI) tø 5.11 min (Method B)
70) N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy} nicotinamide, MS [M+H] = 421.1, LC/MS (EI) tR 2.89 min (Method D)
82) 5-{3-[(5-pyrimidin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate, MS [M+H] = 409.1, LC/MS (EI) tR 4.27 min (Method B)
83) 5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine- 2-carboxylic acid hydroformate, MS [M+H] = 414.1, LC/MS (EI) tR 4.95 min (Method B)
84) 5-{3-[(5-pyrazin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate, MS [M+H] = 409.1, LC/MS (EI) tø 4.11 min (Method B)
96) 5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxamide hydroformate, MS [M+H] = 407.1, LC/MS (EI) tR AM min (Method B)
97) 5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine- 2-carboxamide hydroformate, MS [M+H] = 413.1, LC/MS (EI) tR 5.49 min (Method B)
Example 18 87) Methyl 5-{4-[5-(4-fIuorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinate
Figure imgf000130_0001
[0183] Diisopropyl azodicarboxylate (0.040 g, 0.20 mmol) was added to a mixture of 5-hydroxynicotinic acid methyl ester (0.038 g, 0.25 mmol), 4-[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butan-l-ol (0.060 g, 0.20 mmol), triphenylphosphine (0.052 g, 0.20 mol) and tetrahydrofuran (3.0 mL) at room temperature with stirring. After 16 h, the solvent was removed in vacuo. The residue was diluted with ethyl acetate (50 mL) and washed with sodium bicarbonate (1 x 40 mL). The organic solution was concentrated and purified by column chromatography (1.5-5% methanol/dichloromethane) to give 7 mg (8 %) methyl 5-4-[5-(4- fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxynicotinate as white solid. MS [M+H] = 438.1, LC/MS (EI) tR 4.63 min (Method D), 1H NMR (CDCl3) δ (ppm) 9.02 (s, IH), 8.82 (s, IH), 8.38 (s, IH), 7.68 (s, IH), 7.42-7.35 (m, 2H), 7.34 (s, IH), 7.20-7.10 (m, 2H), 3.97 (s, 3H), 3.88 (t, J = 6.3 Hz, 2H), 2.72 (t, J = 7.2 Hz, 2H), 1.81-1.66 (m, 2H), 1.66- 1.53 (m, 2H). [0184] The following compound was synthesized in a similar manner using different starting materials:
95) 5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-l,3-dihydro- 2H-indol-2-one, MS [M+H] = 436.1, LC/MS (EI) tR 6.98 min (Method D)
Example 19
89) 5-{4-[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy}-N-methyl nicotinamide hydroformate
Figure imgf000131_0001
[0185] Methyl 5-{4-[5-(4-fluorophenyl)thieno[2,3-d]ρyrimidin-4- yl]butoxy}nicotinate (0.010 g, 0.023 mmol) and methylamine (0.124 g, 0.004 mol) were mixed in 1-butanol (2.00 mL) and the resulting mixture subjected to microwave radiation for 2000 seconds at 170 °C, followed by irradiation for 4000 seconds at 180 0C. The solvent was removed by evaporation, and the product purified by preparative HPLC to give 2 mg (20%) 5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy}-N- methylnicotinamide hydroformate as a white solid. MS [M+H] = 437.1, LC/MS (EI) tø 3.55 min (Method D), 1H NMR (10% MeOD/CDCl3) δ (ppm) 8.93 (s, IH), 8.43 (s, IH), 8.20 (s, IH), 7.70 (s, IH), 7.60 (s, IH), 7.40-7.28 (m, 4H), 7.11 (t, J = 8.7 Hz, 2H), 3.84 (t, J = 6.0 Hz, 2H), 2.95 (d, J = 3.6 Hz, 3H), 2.67 (t, J = 7.2 Hz, 2H), 1.75-1.59 (m, 2H), 1.59-1.45 (m, 2H)
Example 20
90) 5-{4-[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy}nicotinamide hydroformate
Figure imgf000131_0002
[0186] Methyl 5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]butoxy}nicotinate (0.015 g, 0.034 mmol) and ammonia (3.00 mL, 20%, 0.02 mol) were mixed in 1-butanol (1.00 mL) and the resulting mixture was heated at 180 °C for 20 h. The solvent was removed by evaporation, and the product waspurified by preparative HPLC to give 5 mg (36 %) 5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]butoxy} nicotinamide hydroformate as a white solid. MS [M+H] = 423.1, LC/MS (EI) tR 3.45 min (Method D), 1H NMR (7% MeOD/CDCl3) δ (ppm) 8.92 (s, IH), 8.50 (s, IH), 8.25 (s, IH), 7.62 (s, IH), 7.40-7.30 (m, 3H), 7.10 (t, J = 8.4 Hz, 2H), 3.84 (t, J = 6.0 Hz, 2H), 2.66 (t, J = 7.5 Hz, 2H), 1.75-1.58 (m, 2H), 1.59-1.45 (m, 2H).
Example 21
91) N-[3-(3-[5-(4-Fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxyphenoxy)phenyl] acetamide
Figure imgf000132_0001
[0187] N-[3-(3-hydroxyphenoxy)phenyl]acetamide (0.032 g, 0.13 mmol) in N,N- dimethylacetamide (2.5 mL) was treated with sodium hydride (0.0063 g, 0.16 mol) at room temperature over a period of 1.5 h. 4-chloro-4-(4-fluorophenyl)thieno[2,3- d]pyrimidine (0.052 g, 0.20 mmol) was added, and the mixture was stirred at room temperature for 2 h. The reaction mixture was then treated with acetic acid (0.2 mL) for 5 min, and the solvent was evaporated in vacuo. The residue was dissolved in ethylacetate (50 mL) and washed with 5% solium bicarbonate (2 x 30 mL). The organic layer was concentrated and the product purified by silica gel chromatography (15% ethyl acetate/hexane) to afford 20 mg (32%) N-[3-(3-[5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-yl]oxyphenoxy)phenyl] acetamide as colorless gum. MS [M+H] = 472, LC/MS (EI) tR 7.57 min (Method D), 1H NMR (CDCl3) δ (ppm) 8.65 (s, IH), 8.50 (s, IH), 7.58-7.48 (m, 2H), 7.35 (m, 2H), 7.33-7.27 (m, 3H), 7.25 (s, IH), 7.21 (s, IH), 7.09 (t, J = 8.7 Hz, 2H), 6.93-6.84 (m, 3H), 6.84-6.74 (m, 2H), 2.17 (s, 3H) [0188] The following compound was synthesized in a similar manner using different starting materials: 93) N-[3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}phenoxy) phenyl] acetamide hydroformate, MS [M+H] = 461, LC/MS (EI) tR 6.19 min (Method D)
Example 22 86) 4-But-3-en-l -yl-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine
Figure imgf000133_0001
[0189] 3-Butenylmagnesium bromide (0.0040 mol) was added to a solution of 4- chloro-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine (0.529 g, 0.00200 mol), copper (I) iodide (0.038 g, 0.00020 mol) and ferrous bromide (0.043 g, 0.00020 mol) in tetrahydrofuran (10 mL) and the resulting mixture was maintained at room temperature for 5 h, then quenched by the addition of water (10 mL). Ethyl acetate (50 mL) was added and the mixture was filtered. The filtrate was washed with a saturated solution of sodium bicarbonate (40 mL) and the organics were concentrated and purified by silica gel column chromatography (using 15% ethyl acetate/hexane as eluent) to afford 4-But-3-en- l-yl-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine as a white solid in 67% yield.
Example 23 85) 4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butan-l-ol
Figure imgf000133_0002
[0190] 9-BBN (0.236 g, 0.00193 mol) in tetrahydrofuran (5.00 mL) was treated with 4-but-3-en-l-yl-5-(4-fluorophenyl)thieno[2,3-d]ρyrimidine (0.500 g, 0.00176 mol) in tetrahydrofuran (3.0 mL) at 50 °C for 1 h. Ethanol (1.0 mL), 6 N sodium hydroxide (0.33 mL) and 30% hydrogen peroxide (0.67 mL) were then added successively and the resulting mixture was maintained at 50 °C for 1 h. The reaction was then cooled to room temperature, diluted with ethyl acetate (50 mL) and washed with sodium bicarbonate (2 x 30 mL). The organic layers were combined, concentrated and purified by column chromatography over silica gel (using ethyl acetate/hexane 1 :5-l :1 as an eluent) to afford 4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butan-l-ol as a white solid in 47% yield.
Example 24
98) N-[4-(3- {[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4yl]amino} propyl)phenyl] acetamide
(i)
Figure imgf000134_0001
[0191] 4-(3-aminopropyl)aniline (0.034 g, 0.23 mmol) was added to a solution of
4-chloro-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine (0.04 g, 0.15 mmol), copper(I) iodide (0.009 g, 0.04 mmol) and potassium carbonate (0.0626 g, 0.453 mmol) in N,N- dimethylacetamide (3 mL) and the resulting mixture was subjected to microwave radiation at 140 0C for 4 hours. The solvent was then removed by evaporation, and the residue was dissolved in ethyl acetate (50 mL), filtered, and the organics were washed with a saturated solution of sodium bicarbonate (30 mL). Concentration and purification by silica gel column chromatography (using 1-3% methanol, 0.06% ammonia in 1 :1 ethyl acetate/hexane) afforded N-[3-(4-aminophenyl)propyl]-5-(4-fluorophenyl)thieno[2,3- d]pyrimidin-4-amine as a white solid in 30% yield. (ϋ)
Figure imgf000135_0001
[0192] A mixture of N-[3-(4-aminophenyl)propyl]-5-(4-fiuorophenyl)thieno[2,3- d]pyrimidin-4-amine (0.014 g, 0.037 mmol) and acetic anhydride (0.01 mL) in methylene chloride (2.0 mL) was heated to 60 0C for 6 h. The reaction was allowed to cool to room temperature and concentrated in vacuo. The residue was then dissolved in ethyl acetate (30 mL) and the organic layer was washed with sodium bicarbonate (2 x 25 mL). Concentration, followed by followed by silica gel column chromatography purification (using 3% methanol in 1 :1 ethyl acetate/hexand and ammonia (0.03%) as eluent) afforded N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4yl]amino} propyl)phenyl]acetamide in 90% yield.
Example 25
55) N-(3-{3-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}phenyl)acetamide
[0193] A mixture of 5-phenylthieno[2,3-d]pyrimidin-4(lH)-one (0.023 g, 0.10 mmol), N-[3-(3-bromopropoxy)phenyl]acetamide (0.0274 g, 0.10 mmol), potassium carbonate (0.0418 g, 0.30 mmol) and N,N-dimethylformamide (1.0 mL) was heated to 90 °C for 6 h. The reaction was then allowed to cool to room temperature and concentrated in vacuo. The residue was dissolved in ethyl acetate (30 mL), water (30 mL) was added, and the organic layer was separated, Concentration, followed by followed by silica gel column chromatography purification (using 1.50 % methanol in 1 :1 ethyl acetate/hexane and ammonia (0.03%) as eluent) afforded N-(3-{3-[(5-phenylthieno[2,3-d]pyrimidin-4- yl)oxy]propoxy}phenyl)acetamide in 66% yield. N-{4-[3-(4-oxo-5-phenylthieno[2,3- d]pyrimidin-l(4H)-yl)propoxy]phenyl} acetamide was also formed in 12% yield during the reaction. Example 26 mPDE10A7 Enzyme Activity and Inhibition
[0194] To analyze the enzyme activity, 5 μl of serial diluted mPDE10A7
(PDE 10A7 isoform enzyme) containing lysate were incubated with equal volumes of diluted (100-fold) fluorescein labeled cAMP or cGMP for 30 minutes in MDC HE 96- well assay plates at room temperature. Both the enzyme and the substrates were diluted in the following assay buffer: Tris/HCl (pH 8.0) 50 mM, MgCl2 5 mM, 2- mercaptoethanol 4 mM, BSA 0.33 mg/ml. After incubation, the reaction was stopped by adding 20 μl of diluted (400-fold) binding reagents and was incubated for an hour at room temperature. The plates were counted in an Analyst GT (Molecular Devices) for fluorescence polarization. An IMAP Assay kit (Molecular Device) was used to assess enzyme properties of mPDE10A7. Data were analyzed with SoftMax Pro. [0195] To check the inhibition profile, 10 μl of serial diluted compounds were incubated with 30μl of diluted PDE enzymes in a 96-well polystyrene assay plate for 30 minutes at room temperature. After incubation, 5 μl of the compound-enzyme mixture were aliquoted into a MDC HE black plate, mixed with 5 μl of 100-fold diluted fluorescein labeled substrates (cAMP or cGMP), and incubated for 30 minutes at room temperature. The reaction was stopped by adding 20 μl of diluted binding reagents and counted in an Analyst GT for fluorescence polarization. The data were analyzed with SoftMax Pro.
[0196] Certain chemical entities described herein show activity with IC50 values of generally less than 5μM, and in certain embodiments, less than 0.5 μM.
Example 27 Apomorphine Induced Deficits in Prepulse Inhibition of the Startle Response in Rats, an in vivo Test for Antipsychotic Activity
[0197] The thought disorders that are characteristic of schizophrenia may result from an inability to filter, or gate, sensorimotor information. The ability to gate sensorimotor information can be tested in many animals as well as in humans. A test that is commonly used is the reversal of apomorphine-induced deficits in the prepulse inhibition of the startle response. The startle response is a reflex to a sudden intense stimulus such as a burst of noise. In this example, rats are exposed to a sudden burst of noise, at a level of 120 db for 40 msec, e.g. the reflex activity of the rats is measured. The reflex of the rats to the burst of noise may be attenuated by preceding the startle stimulus with a stimulus of lower intensity, at 3 to 12 db above background (65 db), which will attenuate the startle reflex by 20 to 80% (Geyer, M. A. and Swerdlow, N. R., Measurement of Startle Response, Prepulse Inhibition and Habituation, Unit 8.7 in Current Protocols in Neuroscience, 2003, John Wiley & Sons). [0198] The prepulse inhibition of the startle reflex, described above, may be attenuated by drugs that affect receptor signaling pathways in the CNS. One commonly used drug is the dopamine receptor agonist apomorphine. Administration of apomorphine will reduce the inhibition of the startle reflex produced by the prepulse. Antipsychotic drugs such as haloperidol will prevent apomorphine from reducing the prepulse inhibition of the startle reflex. This assay may be used to test the antipsychotic efficacy of PDElO inhibitors, as they reduce the apormorphine-induced deficit in the prepulse inhibition of startle. Therefore, PDElO inhibitors may be useful in restoring the deficits in sensorimotor gating that contribute to the thought disorders that characterize schizophrenia.
[0199] The preceding examples can be repeated with similar success by substituting the genetically or specifically described reactants and/or operating conditions of this invention for those used in the preceding examples.
[0200] While the invention has been illustrated with respect to the production and of particular compounds, it is apparent that variations and modifications of the invention can be made without departing from the spirit or scope of the invention. Upon further study of the specification, further aspects, objects and advantages of this invention will become apparent to those skilled in the art.

Claims

WE CLAIM:
1. At least one chemical entity chosen from compounds of Formula I:
Figure imgf000138_0001
(Formula I) and pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, and mixtures thereof, wherein R1 is chosen from:
(i) C6-Io aryl;
(ii) substituted C6-I0 aryl chosen from mono-, di-, and tri-substituted aryl wherein the substituents are independently chosen from halo, Cp8 alkyl, halogenated Cp8 alkyl, Ci-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy C1- 8 alkyl, hydroxy C2-8 alkoxy, amino, Ci -8 alkylamino, di-Cp8 alkylamino, C 1-8 alkylsulphinyl, Cp8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio; (iii) heterocyclyl; (iv) substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl wherein the substitutents are independently chosen from halo, C 1-8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(v) heteroaryl; and (vi) substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl wherein the substitutents are independently chosen from halo, Cp8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cj-g alkyl, hydroxy C2-g alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Ci-8 alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
R2 is chosen from C2-4hydroxyalkyl, C2-4 alkenyl, C2-4 alkenylene-R5, C2-4 alkynyl, C2-4 alkynylene-R5, -O-arylene-Y-R5, -X(CR3R4)nYR5 and - (CR6R7)mYR5 where:
X is chosen from -O-, -S- and -NR9- where R9 is chosen from H, Ci-8 alkyl; and C3-8 cycloalkyl, substituted Ci-g alkyl; and substituted C3-8 cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Q-4-alkyl, Ci-4-alkoxy, and oxo;
Y is chosen from a covalent bond, -O-, -S- and -NR9-; n is chosen from 2, 3, 4, and 5; m is chosen from 2, 3, 4, and 5;
R and R4 are each independently chosen from: (i) H; (ii) halo; (iii) C1-12 alkyl; (iv) C2-I2 alkenyl; (v) C2-I2 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted CM2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl and wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated C1-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-CM-alkylamino, C1-4-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated CM alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci_4-hydroxyalkyl, C2-4-hydroxyalkoxy, CM-alkylthio, Ci-4-alkylsulphinyl, and Ci-4- alkylsulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, C1-4- hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C^-alkylsulphinyl, and Ci_4-alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci ^-alkylamino, Q-4- hydroxyalkyl, C2-4-hydroxyalkoxy, C1-4-alkylthio, Q^-alkylsulphinyl, and Ci-4-alkylsulphonyl;
R6 and R7 are independently chosen from (i) H; (ii) halo; (iii) CM2 alkyl; (iv) C2-12 alkenyl; (v) C2-12 alkynyl; (vi) C3-I2 cycloalkyl;
(vii) substituted Ci-I2 alkyl chosen from mono-, di-, and tri-substituted CM2 alkyl wherein the substituents are independently chosen from halo, hydroxy, Q ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-C1-4-alkylamino, Ci-4-hydroxyalkyl, C2-4- hydroxyalkoxy, Ci-4-alkylthio, Ci-4-alkylsulphinyl, and Q-4- alkylsulphonyl;
(viii) substituted C2-I2 alkenyl chosen from mono-, di-, and tri- substituted C2-I2 alkenyl wherein the substituents are independently chosen from halo, hydroxy, Ci^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, C^-hydroxyalkyl, C2-4-hydroxyalkoxy, Ci-4-alkylthio, C]-4-alkylsulphinyl, and Ci-4- alkyl sulphonyl;
(ix) substituted C2-I2 alkynyl chosen from mono-, di-, and tri- substituted C2-I2 alkynylwherein the substituents are independently chosen from halo, hydroxy, Ci ^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino,
Figure imgf000141_0001
C2-4-hydroxyalkoxy, CM-alkylthio, CM-alkylsulphinyl, and Ci-4- alkylsulphonyl; and
(x) substituted C3-I2 cycloalkyl chosen from mono-, di-, and tri- substituted C3-I2 cycloalkyl wherein the substituents are independently chosen from halo, hydroxy, C^-alkoxy, halogenated Ci-4 alkoxy, nitro, cyano, carboxy, amino, Ci-4 alkylamino, di-Ci-4-alkylamino, Ci-4- hydroxyalkyl, C2-4-hydroxyalkoxy, C^-alkylthio, Ci-4-alkylsulphinyl, and Cm-alkylsulphonyl; and
R5 is chosen from C6-I0 aryl, substituted C6-I0 aryl, Het, substituted Het, C6-I0 aryl- Ci-s alkyl, substituted C6-I0 aryl-Ci-g alkyl, Het-Ci-8 alkyl, and substituted Het-Ci-8 alkyl, wherein the alkyl portions of C6-I0 aryl-Q-g alkyl and Het- C i-g alkyl are optionally substituted by oxo and wherein: substituted C6-io aryl is chosen from mono-, di-, and tri-substituted C6-I0 aryl wherein the substituents are independently chosen from
(1) C-8 alkyl,
(2) C3-8 cycloalkyl,
(3) C4-S cycloalkylalkyl,
(4) Ci-8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro, (12) C1-S halogenated alkyl,
(13) C1-S halogenated alkoxy,
(14) C i -8 hydroxyalkyl,
(15) C2-8 hydroxyalkoxy,
(16) C3-S alkenyloxy,
(17) Cr8 alkylamino,
(18) di-C i -8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-8 alkylthio,
(37) Ci -8 alkylsulphinyl,
(38) C1-S alkylsulphonyl,
(39) C1-S alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl, (43) alkylaminosulfonyl,
(44) C6-H, aryl,
(45) substituted C6-Io aryl chosen from mono-, di-, and trisubstituted C6- io aryl wherein the substituents are independently chosen from Ci-8 alkyl, halogenated Ci -8 alkyl, Ci-4 alkoxy, halogenated Ci-4 alkoxy, Ci-g hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Ci-g alkylsulphinyl, Ci-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio;
(46) heterocyclyl;
(47) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substitutents are independently chosen from halo, Ci-8 alkyl, halogenated Ci-8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Q-g alkylamino, di-Ci-g alkylamino, Q-8 alkylsulphinyl, Cr8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio;
(48) heterocyclyl-carbonyl; and
(49) heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Crs alkyl, halogenated Cr8 alkyl, C1-4 alkoxy, halogenated Ci- 4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, Q-g alkylamino, di-Ci-8 alkylamino, Ci-8 alkylsulphinyl, Cr8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio; and
Het is chosen from heterocyclyl, substituted heterocyclyl chosen from mono-, di-, and tri-substituted heterocyclyl, heteroaryl, and substituted heteroaryl chosen from mono-, di-, and tri-substituted heteroaryl, wherein the substituents are independently chosen from
(1) Cr8 alkyl,
(2) C3-8 cycloalkyl,
(3) C4-8 cycloalkylalkyl, (4) Cr8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(H) nitro,
(12) Ci-8 halogenated alkyl,
(13) Ci-8 halogenated alkoxy,
(14) Ci-g hydroxyalkyl,
(15) C2-8 hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) Ci-g alkylamino,
(18) di-d-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-, (35) acyloxy,
(36) Cp8 alkylthio,
(37) Ci-8 alkylsulphinyl,
(38) C1-S alkylsulphonyl,
(39) Ci -8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-2o aryl,
(46) substituted C6-2o aryl chosen from mono-, di-, and tri-substituted C6-20 aryl wherein the substituents are independently chosen from halo, C i-g alkyl, halogenated Ci-s alkyl, C 1-4 alkoxy, halogenated C1 -4 alkoxy, Ci-g hydroxyalkyl, C2-8 hydroxyalkoxy, amino, Cp8 alkylamino, di-Ci-g alkylamino, Ci-8 alkylsulphinyl, Cp8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Ci-g alkyl, halogenated Ci-8 alkyl, C1-4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cι-% alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cp8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Q-8 alkyl, halogenated Cp8 alkyl, Cp4 alkoxy, halogenated Ci-4 alkoxy, hydroxy Ci-8 alkyl, hydroxy C2-8 alkoxy, amino, C i-8 alkyl amino, di-Ci-8 alkylamino, Cr8 alkylsulphinyl, C1-S alkylsulphonyl, Ci -8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Ci-8 alkyl, halogenated Cps alkyl, Cp4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Q-salkylamino, Ci-8 alkylsulphinyl, d-8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio, and
R9 is chosen from H, Cr8 alkyl, C3-8 cycloalkyl, substituted Cp8 alkyl chosen from mono-, di-, and trisubstituted Cp8 alkyl, and substituted C3-8 cycloalkyl chosen from mono-, di-, and trisubstituted C3-8 cycloalkyl wherein the substituents are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo; and provided that the compound of Formula I is not chosen from :
(i) when R1 is 4-methylphenyl, then R2 is not -O-p-phenylene-0- (CH2)phenyl, -O-p-phenylene-NH-C(O)-thiophen-2-yl, -O-(CH2)2-O-(phenyl), - NH(CH2)2-(4-hydroxyphenyl), -O-(CH2)2-(l,3-dioxoisoindolin-l-yl), -NH(CH2)2-(2-oxo- imidaziolidin-1-yl), -NH(CH2)2-(3,4,-dimethoxyphenyl), -S(CH2)2-phenyl, -NH(CH2)2- phenyl, -NH(CH2)2-morpholin-4-yl, -NH(CH2)2-thiophen-2-yl, -NH(CH2)2-(3,4- methylenedioxyphenyl), -NH(CH2)3-morpholin-4-yl, or-S(CH2)3-phenyl;
(ii) 2-methoxy-4-[[[2-[(5-phenylthieno[2,3-d]pyimidin-4- yl)amino] ethyl] iminoj-methyl] -phenol ; (iii) 4,4'-[ 1 ,3-phenylenebis(oxy)bis[5-(4-chlorophenyl)-thieno[2,3- d]pyrimidine;
(iv) when R1 is phenyl, then R2 is not -O-p-phenylene-NH-C(O)-3,-4- dimethoxyphenyl, -O-(CH2)2-O-(4-methylphenyl), -O-(CH2)2-Ophenyl, -O-(CH2)2-O(3- bromophenyl), -NH-(CH2)2-NH(3-CF3-pyridin-2-yl), -S-(CH2)2-O-(3-methylphenyl), -S- (CH2)2-O-(4-Fphenyl), -S-(CH2)2-0-(2,4-dichlorophenyl), -NH-(CH2)2-NH(4-CF3- pyrimidin-2-yl), -NH-(CH2)2-O-(3-acetylaminophenyl), -S-(CH2)3-phenyl, -S-(CH2)4- (1,3-dioxoisoindolin-l-yl), -NH-(CH2)3-moφholin-4-yl, -NH-(CH2)3-imidazol-l-yl, -NH- (CH2)3-(2-oxo-pyrrolidin-l-yl), -N(CH3)-(CH2)2-pyridin-2-yl, -NH-(CH2)2-pyridin-2-yl, - S-(CH2)2-(l,3-dioxoisoindolin-l-yl, -S-(CH2)2-phenyl, -NH-(CH2)2-(2-chlorophenyl), - NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-(3 ,4-dimethoxyphenyl), -NH-(CH2)2-thiophen- 2-yl, or-NH-(CH2)2-phenyl;
(v) when R1 is 4-fluorophenyl, then R2 is not chosen from -O-(CH2)2-O-(2- chlorophenyl), -O-(CH2)2-O-(4-fluorophenyl), -S-(CH2)3-phenyl, -NH-(CH2)3-phenyl, - NH-(CH2)2-phenyl, -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)3-(2-oxo-pyrrolidin-l-yl), - NH-(CH2)2-morpholin-4-yl, -S-(CH2)2-phenyl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2- morpholin-4-yl, -NH-(CH2)2-(3,4-methylenedioxyphenyl), -NH-(CH2)2-thiophen-2-yl, and -NH-(CH2)2-(3 ,4-ethylenedioxyphenyl);
(vi) when R1 is 4-chlorophenyl, then R2 is not chosen from -O-p-phenylene-O- CH2-phenyl, -O-(CH2)2-O-(4-methylphenyl), -S-(CH2)4-(l,3-dioxoisoindolin-l-yl), -S- (CH2)3-phenyl, -S-(CH2)2-phenyl, -NH-(CH2)3-imidazol-l-yl, -NH-(CH2)3-morpholin-4- yl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-azepan-l-yl, -S-(CH2)2-(1,3- dioxoisoindolin-1-yl), -NH-(CH2)2-(3,4-methylenedioxyphenyl), -NH-(CH2)2-phenyl, - NH-(CH2)2-thiophen-2-yl, -NH-(CH2)2-(3,4-ethylenedioxyphenyl), -S-(CH2)4-(1 ,3- dioxoisoindolin-1-yl), -NH(CH2)3-(2-oxo-pyrrolidin-l-yl), and -NH(CH2)2-(3,4,- dimethoxyphenyl) ;
(vii) when R1 is 3-methylphenyl, then R2 is not -O-(CH2)2-O-(phenyl);
(viii) when R1 is 3-thiophen-2-yl, then R2 is not chosen from -NH-(CH2)2- morpholin-4-yl, -NH-(CH2)3-morpholin-4-yl, -NH-(CH2)2-phenyl, and -NH-(CH2)2-(3 ,4- dimethoxyphenyl);
(ix) when R1 is 4-methoxyphenyl, then R2 is not chosen from -S-(CH2)3- phenyl, -NH-(CH2)2-morpholin-4-yl, -NH-(CH2)2-C(CH3)2-morpholin-4-yl, - NHCH2C(CH3)2-morpholin-4-yl, -N(CH3)-(CH2)2-(3 ,4-dimethoxyphenyl), -S-(CH2)2- phenyl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(x) when R1 is 3,4-dimethylphenyl, then R2 is not chosen from -O-p- phenylene-O-CH2-phenyl, -S-(CH2)3-phenyl, -S-(CH2)3-morpholin-4-yl, -S-(CH2)2- phenyl, -NH-(CH2)3-(4-hydroxyphenyl), -O-(CH2)2-(l,3-dioxoisoindolin-l-yl), -NH- (CH2)2- morpholin-4-yl, -NH-(CH2)3- moφholin-4-yl, -NH-(CH2)2-(3,4- methylenedioxyphenyl), -S-(CH2)2-phenyl, -NH-(CH2)2-phenyl, -NH-(CH2)2-thiophen-2- yl, and -NH-(CH2)2-(3,4-methylenedioxyphenyl);
(xi) when R1 is 4-bromophenyl, then R2 is not chosen from -(Ct^rphenyl, -S- (CH2)3-moφholin-4-yl, -S-(CH2)2-phenyl, -S-(CH2)3-phenyl, -NH-(CH2)3-moφholin-4- yl, -NH-(CH2)3-(2-oxo-pyrτolidin-l-yl), and -NH-(CH2)2-(3,4-ethylenedioxyphenyl); and
(xii) when R1 is 4-biphenyl, then R2 is not -NH-(CH2)2-(3,4- ethylenedioxyphenyl);
(xiii) when R1 is 2,3-dihydro-l ,4-benzodioxin-2-yl, then R2 is not NH(CH2)2- phenyl, and
(xiv) 5-(2-chlorophenyl)-4-[4-(phenylmethoxy)phenoxy]-thieno[2,3- djpyrimidine.
2. At least one chemical entity chosen from claim 1 wherein
R5 is chosen from pyridin-3-yl and pyridin-4-yl, each of which is optionally substituted with one or two groups chosen from
(1) C,-8 alkyl,
(2) C3-S cycloalkyl,
(3) C4-8 cycloalkylalkyl,
(4) C,-8 alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino, (9) cyano,
(10) hydroxyl,
(11) nitro,
(12) Ci-shalogenated alkyl,
(13) Ci-8 halogenated alkoxy,
(14) C]-S hydroxyalkyl,
(15) C2-S hydroxyalkoxy
(16) C3-8 alkenyloxy,
(17) Ci-s alkylamino,
(18) di-Ci-8 alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-g alkylthio,
(37) Ci-g alkylsulphinyl,
(38) Ci-8 alkylsulphonyl,
(39) Ci-8 alkylsulphonamido, (40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-20 aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-2o aryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, Ci -4 alkoxy, halogenated Ci -4 alkoxy, Crghydroxyalkyl, C2-8 hydroxyalkoxy, amino, Crs alkylamino, di-Cr8 alkylamino, Cr8 alkyl sulphinyl, Crs alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, Cj-4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Cr8 alkylamino, Cr8 alkylsulphinyl, Cr8 alkylsulphonyl, Crs alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Ci -8 alkyl, C 1-4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, Cr8 alkylsulphinyl, Cr8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cj-8 alkyl, halogenated C1-S alkyl, Cr4 alkoxy, halogenated C1-4 alkoxy, hydroxy C1-S alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Cr8 alkyl, C1-4 alkoxy, halogenated C1-4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-d-8 alkylamino, Crs alkylsulphinyl, C1-S alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
3. At least one chemical entity of claim 1 wherein R5 is chosen from pyridin-2-yl and pyridin-4-yl, each of which is substituted with one or two groups chosen from
(1) Cr8 alkyl,
(2) C3-8 cycloalkyl,
(3) C4-S cycloalkylalkyl,
(4) C1-S alkoxy,
(5) C3-8 cycloalkoxy,
(6) C4-8 cycloalkylalkoxy,
(7) halo,
(8) amino,
(9) cyano,
(10) hydroxyl,
(11) nitro,
( 12) C2-S halogenated alkyl,
(13) Cr8 halogenated alkoxy,
(14) Crs hydroxyalkyl,
(15) C2-8 hydroxyalkoxy (16) C3-S alkenyloxy,
(17) C1-S alkylamino,
(18) di-Ci-g alkylamino,
(19) carboxy,
(20) alkoxycarbonyl,
(21) carboxamido,
(22) aminocarbonyl,
(23) hydroxyaminocarbonyl,
(24) alkylaminocarbonyl,
(25) dialkylaminocarbonyl,
(26) urea,
(27) hydroxyurea,
(28) alkylurea,
(29) dialkylaminoalkylcarbonyl,
(30) aminocarbonylalkylaminocarbonyl,
(31) acylamido,
(32) HCO-NH-NH-CO-,
(33) NH2NHCO-,
(34) NH2NHCONH-,
(35) acyloxy,
(36) Ci-8 alkylthio,
(37) Ci-β alkylsulphinyl,
(38) Ci-s alkylsulphonyl,
(39) Ci-8 alkylsulphonamido,
(40) (alkylsuphonyl)2amino-,
(41) thiol,
(42) aminosulfonyl,
(43) alkylaminosulfonyl,
(44) oxo,
(45) C6-20 aryl,
(46) substituted C6-20 aryl chosen from mono-, di-, and tri-substituted C6-2o aryl wherein the substituents are independently chosen from halo, Ci-8 alkyl, halogenated Cp8 alkyl, d-4 alkoxy, halogenated Cr4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Ci-8 alkylamino, di-Ci-8 alkylamino, Ci-g alkylsulphinyl, Ci-8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(47) heterocyclyl,
(48) substituted heterocyclyl chosen from mono-, di-, and trisubstituted heterocyclyl wherein the substituents are independently chosen from halo, C1-S alkyl, halogenated Cr8 alkyl, C r4 alkoxy, halogenated Ci -4 alkoxy, hydroxy Cr8 alkyl, hydroxy C2-8 alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, C1-S alkylsulphinyl, Crs alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(49) heteroaryl,
(50) substituted heteroaryl chosen from mono-, di-, and trisubstituted heteroaryl wherein the substituents are independently chosen from halo, Cr8 alkyl, halogenated Crs alkyl, Cr4 alkoxy, halogenated Cr4 alkoxy, hydroxy C1-S alkyl, hydroxy C2-g alkoxy, amino, Cr8 alkylamino, di-Crs alkylamino, d-8 alkylsulphinyl, Crs alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(51) heterocyclyl-carbonyl,
(52) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independently chosen from halo, Crs alkyl, halogenated C1-S alkyl, Cj-4 alkoxy, halogenated Ci-4 alkoxy, hydroxy C1-S alkyl, hydroxy C2-8 alkoxy, amino, C1-S alkylamino, di-Q-g alkylamino, C1-S alkylsulphinyl, C1-S alkylsulphonyl, C1-S alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(53) heteroaryl-carbonyl, and
(54) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo, Cp8 alkyl, halogenated C1-S alkyl, Cp4 alkoxy, halogenated Cp4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cj-g alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cr8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
4. At least one chemical entity of claim 1 wherein the compound of Formula I is chosen from compounds of Formula II:
Figure imgf000154_0001
(Formula II)
wherein p is chosen from 1, 2, 3, and 4; and for each occurrence, R10 is independently chosen from
(1) C2-S alkyl,
(2) C3-8 cycloalkyl,
(3) C4-S cycloalkylalkyl,
(4) C3-8 cycloalkoxy,
(5) C4-S cycloalkylalkoxy,
(6) amino,
(7) cyano,
(8) nitro,
(9) C i -8 halogenated alkyl,
(10) C i -g halogenated alkoxy, (H) Ci-8hydroxyalkyl,
(12) C2-8 hydroxyalkoxy,
(13) Cp8 alkylamino,
(14) di-Ci -8alkylamino,
(15) carboxy,
(16) alkoxycarbonyl,
(17) aminocarbonyl,
(18) hydroxyaminocarbonyl,
(19) alkylaminocarbonyl,
(20) dialkylaminocarbonyl,
(21) urea,
(22) hydroxyurea,
(23) alkylurea,
(24) dialkylaminoalkylcarbonyl,
(25) aminocarbonylalkylaminocarbonyl,
(26) acylamido,
(27) HCO-NH-NH-CO-,
(28) NH2NHCO-,
(29) NH2NHCONH-,
(30) acyloxy,
(31) Ci-8 alkylthio,
(32) Cr8 alkylsulphinyl,
(33) C 1-8 alkylsulphonyl,
(34) C 1-8 alkylsulphonamido,
(35) (alkylsuphonyl)2amino-,
(36) thiol,
(37) aminosulfonyl,
(38) alkylaminosulfonyl,
(39) C6-20 aryl,
(40) substituted C6-20 aryl chosen from mono-, di-, and tri-substiruted
C6-20 aryl wherein the substituents are independently chosen from halo, C i-8 alkyl, halogenated Ci-8 alkyl, C 1-4 alkoxy, halogenated C 1-4 alkoxy, Ci-ghydroxyalkyl, C2-8 hydroxyalkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Ci-8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, and thio,
(41) heterocyclyl-carbonyl,
(42) substituted heterocyclyl-carbonyl chosen from mono-, di-, and trisubstituted heterocyclyl-carbonyl wherein the substituents are independentlychosen from halo, Cp8 alkyl, halogenated C 1-8 alkyl, Cp4 alkoxy, halogenated C1-4 alkoxy, hydroxy Cps alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Ci-8 alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, C\s alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio,
(43) heteroaryl-carbonyl, and
(44) substituted heteroaryl-carbonyl chosen from mono-, di-, and trisubstituted heteroaryl-carbonyl wherein the substituents are independently chosen from halo,Cp8 alkyl, halogenated Cp8 alkyl, C\-4 alkoxy, halogenated C 1-4 alkoxy, hydroxy Cp8 alkyl, hydroxy C2-8 alkoxy, amino, Cp8 alkylamino, di-Cp8alkylamino, Cp8 alkylsulphinyl, Cp8 alkylsulphonyl, Cp8 alkylsulphonamido, -COR9, -CSR9, -SR9, cyano, hydroxyl, nitro, oxo, and thio.
5. At least one chemical entity of any one of claims 1 to 3 wherein R2 is chosen from -O-arylene-Y-R5, -X(CR3R4)nYR5 and -(CR6R7)mYR5.
6. At least one chemical entity of claim 5 wherein R2 is -X(CR3R4)nY-R5.
7. At least one chemical entity of claim 5 wherein R2 is -O-arylene-Y-R5.
8. At least one chemical entity of claim 6 wherein R2 is -O-phenylene-Y-R5.
9. At least one chemical entity of claim 8 wherein the -O and -YR5 substituents attached the phenylene group are meta-substituted.
10. At least one chemical entity of claim 5 wherein R2 is -(CR6R7)mYR5.
11. At least one chemical entity of any one of claims 1 to 6 wherein X is -NH-.
12. At least one chemical entity of any one of claims 1 to 6 wherein X is -O-.
13. At least one chemical entity of any one of claims 1 to 12 wherein Y is a bond.
14. At least one chemical entity of any one of claims 1 to 12 wherein Y is -O-.
15. At least one chemical entity of any one of claims 1 to 12 wherein Y is -NH-.
16. At least one chemical entity of any one of claims 1 to 15 wherein n is chosen from 3, 4, and 5.
17. At least one chemical entity of any one of claims 1 to 16 wherein m is chosen from 3, 4, and 5.
18. At least one chemical entity of any one of claims 1 to 17 wherein R and R4 are independently chosen from H, CH3, and F.
19. At least one chemical entity of claim 18 wherein R and R4 are H.
20. At least one chemical entity of claims 1 to 19 wherein R6 and R7 are independently chosen from H, CH3, and F.
21. At least one chemical entity of claim 20 wherein R and R7 are H.
22. At least one chemical entity of any one of claims 1 to 3 wherein -(CR6R7)mY-R5 is -(CH2)4-O-R5.
23. At least one chemical entity of any one of claims 1 to 3 wherein -X(CR3R4)nY-R5 is chosen from -NH-(CH2)3-O-R5, -NH-(CH2)2-NH-R5, -O-(CH2)2-O-R5, -O-(CH2)3-O- R5, -O-CH2-C(CH3)2-CH2-O-R5, and-NH-(CH2)2-O-R5.
24. At least one chemical entity of claim 23 wherein X(CR3R4)nY-R5 is chosen from -NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5.
25. At least one chemical entity of any one of claims 1 to 3 wherein -X(CR3R4)nYR5 is -NH-(CH2)3-R5.
26. At least one chemical entity of claim 1 wherein the compound of Formula I is chosen from compounds of Formula (Ia):
Figure imgf000158_0001
(Formula Ia) wherein:
R2 is -chosen from X(CR3R4)nYR5 and -(CR6R7)mYR5; and Y is chosen from O, S, and NR9.
27. At least one chemical entity of any one of claims 1 to 26 wherein R1 is chosen from aryl and substituted aryl wherein substituted aryl is chosen from mono-, di-, and tri- substituted aryl.
28. At least one chemical entity of claim 27 wherein R1 is chosen from phenyl and substituted phenyl wherein substituted phenyl is chosen from mono-, di-, and tri- substituted phenyl.
29. At least one chemical entity of claim 28 wherein R1 is chosen from phenyl and phenyl substituted with one or more halo.
30. At least one chemical entity of claim 29 wherein R1 is chosen from phenyl and 4- F-phenyl.
31. At least one chemical entity of any one of claims 1 to 26 wherein R1 is chosen from heterocyclyl, substituted heterocyclyl, heteroaryl, and substituted heteroaryl.
32. At least one chemical entity of claim 31 wherein R1 is chosen from optionally substituted furanyl, optionally substituted pyridinyl, optionally substituted pyrimidinyl, optionally substituted pyrazinyl, and optionally substituted thiazolyl.
33. At least one chemical entity of claim 32 wherein R1 is chosen from 5-chloro- furan-2-yl, furan-2-yl, pyridin-4-yl, pyridin-3-yl, pyridin-2-yl, l,3-thiazol-2-yl, 5- pyrimidin-2-yl and 5-pyrazin-2-yl.
34. At least one chemical entity of any one of claims 1 to 33 wherein R9 is chosen from H, Ci-4 alkyl; and C3-8 cycloalkyl, substituted Ci-4 alkyl chosen from mono-, di-, and tri-substituted C 1-4 alkyl; and substituted C3-8 cycloalkyl chosen from mono-, di-, and tri-substituted C3-S cycloalkyl, wherein the substituents on the alkyl and cycloalkyl groups are independently chosen from halo, Ci-4-alkyl, Ci-4-alkoxy, and oxo.
35. At least one chemical entity of claim 1 wherein R1 is chosen from aryl, substituted aryl chosen from mono-, di-, and trisubstituted aryl wherein the substitutent is halo, heterocyclyl, and substituted heterocyclyl; R2 is-X(CR3R4)nY-R5; -X(CR3R4)nY- is chosen from -NH-(CH2)3-O-, -NH-(CH2)2-NH-, -O-(CH2)2-O-, -O- (CH2)3-O-, -O-CH2-C(CH3)2-CH2-O-, and -NH-(CH2)2-O-;
R3, R4, R6, and R7 are independently chosen from H, CH3, and F;
X is chosen from O and NR9,
Y is chosen from O and NR9; and
R5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzyl chosen from mono-, di-, and tri-substituted benzyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzoyl chosen from mono-, di-, and tri-substituted benzoyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzothiadiazolyl chosen from mono-, di-, and tri-substituted benzothiadiazolyl wherein the substituents are independently chosen from alkyl- CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO- NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH- CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted pyrazolyl chosen from mono-, di-, and tri-substituted pyrazolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl,
substituted benzisothiazolyl chosen from mono-, di-, and tri-substituted benzisothiazolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO- NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2- NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and moipholinylcarbonyl, substituted dihydroindolyl chosen from mono-, di-, and tri-substituted dihydroindolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO- NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2- NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alky^NH-alkylene-NH-CO-^alky^zN-alkylene-NH-CO-^Hz-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and moipholinylcarbonyl, and substituted pyridinyl chosen from mono-, di-, and tri-substituted pyridinyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
36. At least one chemical entity of claim 1 wherein R1 is chosen from aryl, substituted aryl, heterocyclyl, and substituted heterocyclyl; R2 is -X(CR3R4)nY-R5;
-X(CR3R4)nY- is chosen from -NH-(CH2)3-O-, -NH-(CH2)2-NH-, -O-(CH2)3-O-, and-NH- (CH2)2-O-;
R3, R4, R6, and R7 are independently chosen from H, CH3, and F;
R5 is chosen from phenyl, benzyl, benzoyl, benzothiadiazolyl, pyrazolyl, benzisothiazolyl, dihydroindolyl, pyridinyl, substituted phenyl chosen from mono-, di-, and tri-substituted phenyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzyl chosen from mono-, di-, and tri-substituted benzyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted benzoyl chosen from mono-, di-, and tri-substituted benzoyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and moφholinylcarbonyl, substituted benzothiadiazolyl chosen from mono-, di-, and tri-substituted benzothiadiazolyl wherein the substituents are independently chosen from alkyl- CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO- NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH- CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted pyrazolyl chosen from mono-, di-, and tri-substituted pyrazolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl,
substituted benzisothiazolyl chosen from mono-, di-, and tri-substituted benzisothiazolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO- NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2- NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, substituted dihydroindolyl chosen from mono-, di-, and tri-substituted dihydroindolyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO- NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2- NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH- CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl, and substituted pyridinyl chosen from mono-, di-, and tri-substituted pyridinyl wherein the substituents are independently chosen from alkyl-CO-NH-, NH2-CO-NH-, alkyl- NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH- alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO- NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl
37. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, pyrimidinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, substituted pyrimidinyl, and substituted thiazolyl, and R2 is -X(CR3R4)nYR5.
38. At least one chemical entity of claim 1 wherein R1 is chosen from furanyl, pyridinyl, thiazolyl, phenyl, and phenyl substituted by halo, and R2 is -X(CR3R4)nYR5.
39. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo, and R2 is -X(CR3R4)nYR5.
40. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl, and R2 is chosen from -NH-(CH2)3-O-R5, -NH-(CH2)2-NH-R5, -O- (CH2)3-O-R5, and -NH-(CH2)2-O-R5.
41. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R is chosen from -NH-(CH2)3- O-R5, -NH-(CH2)2-NH-R5, -O-(CH2)3-O-R5, and -NH-(CH2)2-O-R5.
42. At least one chemical entity of claim 1 wherein R is chosen from phenyl and p phheennyyll s suubbssttiittuutteedd b byy h haalloo,, a anndd R R22 i iss c chhoosseenn from -NH-(CH2)3-O-R5, -NH-(CH2)2-NH- R5, -O-(CH2)3-O-R5, and -NH-(CH2)2-O-R5.
43. At least one chemical entity of claim 1 wherein R is chosen from phenyl, furanyl, pyridinyl, or thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl, and R2 is chosen from NH-(CH2)3-O-R5 and -O- (CH2)3-O-R5.
44. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R is chosen from NH-(CH2)3- O-R5 and -O-(CH2)3-O-R5.
45. At least one chemical entity of claim 1 wherein R is chosen from phenyl and phenyl substituted by halo, and R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5.
46. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, which in each case is unsubstiruted or substituted, and R2 is -O-(CH2)3-O-R5.
47. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl, and R2 is -O-(CH2)3-O-R5.
48. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo, and R2 is -O-(CH2)3-O-R5.
49. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substitutedthiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5; and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
50. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5, and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, and pyridinyl,substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
51. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo; R2 is chosen from NH-(CH2)3-O-R5 or -O-(CH2)3-O-R5; and R5 is chosen from phenyl, benzyl, benzoyly, benzothiadiazolyl, pyridinyl, substituted phenyl, substituted benzyl, substituted benzoyly, substituted benzothiadiazolyl, and substituted pyridinyl.
52. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, and thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyONH-alkylene-NH-CO-^alkyOzN-alkylene-NH-CO-^^-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
53. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, phenyl substituted by halo, furanyl, pyridinyl, and thiazolyl; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO- , NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyll.
54. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo; R2 is chosen from NH-(CH2)3-O-R5 and -O-(CH2)3-O-R5; and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO- NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO- , NH2-NH-CO-, HO-NH-CO-, alkyl-NH-CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N-alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl-SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl,.
55. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo; R2 is NH-(CH2)2-O-R5, and R5 is chosen from phenyl and phenyl substituted by alkyl-CO-NH-.
56. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and phenyl substituted by halo; R2 is NH-(CH2)3-O-R5, and; R5 is chosen from benzyl, benzoyly, and benzothiadiazolyl, substituted benzyl, substituted benzoyly, and substituted benzothiadiazolyl, which in each case the substituents are chosen from nitro, alkoxy, cyano, halogenated alkoxy, alkoxycarbonyl, and alkyl-CO-NH-.
57. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl, furanyl, pyridinyl, thiazolyl, substituted phenyl, substituted furanyl, substituted pyridinyl, and substituted thiazolyl; R2 is -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH- CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N- alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl- SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl
58. At least one chemical entity of claim 1 wherein R1 is chosen from phenyl and halogenated phenyl; R2 is -O-(CH2)3-O-R5, and R5 is chosen from phenyl and pyridinyl, which in each case is substituted by alkyl-CO-NH-, NH2-CO-NH-, alkyl-NH-CO-NH-, NH2-NH-CO-NH-, OH-NH-CO-NH-, NH2-CO-, NH2-NH-CO-, HO-NH-CO-, alkyl-NH- CO-, (alkyl)2N-CO-, NH2-alkylene-NH-CO-, (alkyl)NH-alkylene-NH-CO-, (alkyl)2N- alkylene-NH-CO-, NH2-alkylene-NH-CO-, HCO-NH-NH-CO-, alkyl-SO2-NH-, (alkyl- SO2)2N -, nitro, alkyl, alkoxy, halogenated alkoxy, cyano, alkoxycarbonyl, triazolyl, halo, amino, carboxy, methylpiperazinyloxycarbonyl, and morpholinylcarbonyl.
59. At least one chemical entity of claim 1 wherein the compound of Formula I is chosen from
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenylj-acetamide,
N-(3-nitrobenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2-diamine, N-(3,4-dimethoxybenzyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4-yl)ethane-l,2- diamine,
N-(2,l,3-benzothiadiazol-5-ylmethyl)-N'-(5-phenylthieno[2,3-d]pyrimidin-4- yl)ethane- 1 ,2-diamine,
N- {3-[( {2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl} amino)methyl]- phenyl } -acetamide,
N-{4-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- phenyl } -acetamide,
3-(acetylamino)-N-{2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]- ethyl}benzamide,
3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)methyl]- benzonitrile, methyl 3-[({2-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)amino]ethyl}amino)- methyl]-benzoate,
N-(5-phenylthieno[2,3-d]pyrimidin-4-yl)-N'-[3-(trifluoromethoxy)benzyl]ethane- 1 ,2-diamine,
5-(4-fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3-d]pyrimidine,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] -acetamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide,
5-(4-fluorophenyl)-4-{3-[4-(lH-l,2,4-triazol-l-yl)phenoxy]propoxy}thieno[2,3- d]pyrimidine,
4-[3-(4-fluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, methyl 3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoate,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)aniline,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzoic acid,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- cyclopropanecarboxamide,
5-(4-fluorophenyl)-4-(3-{3-[(4-methylpiperazin-l-yl)carbonyl]phenoxy}- propoxy)-thieno[2,3-d]pyrimidine,
5-(4-fluorophenyl)-4-{3-[3-(morpholin-4-ylcarbonyl)phenoxy]propoxy}- thieno[2,3-d]pyrimidine,
N-ethyl-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N,N- dimethylbenzamide,
N-[2-(dimethylamino)ethyl]-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)benzamide,
N-(2-amino-2-oxoethyl)-3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl] oxy} propoxy)benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N'- formylbenzohydrazide,
3 -(3 - { [ 5 -(4-fluorophenyl)thieno [2 , 3 -d] pyrimidin-4-yl] oxy } propoxy)-N- methylbenzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzamide,
3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)-N- methylbenzamide,
N-[3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)phenyl]- urea,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyljurea,
3-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)benzamide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzohydrazide,
3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- hydroxybenzamide,
4-(2-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}ethoxy)benzamide
N-ethyl-N'-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]urea,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- methanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- methanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N-(methylsulfonyl)methansulfonamide,
N-(ethylsulfonyl)-N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}- propoxy)phenyl]ethanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- ethanesulfonamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-methylurea,
N-ethyl-N'-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- phenyl]-urea,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- ethanesulfonamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-methylurea,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- hydrazinecarboxamide,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- N'-hydroxyurea,
4-{3-[5-(4-fluorophenyl)-thieno[2,3d]pyrimidin-4-yloxy]-propoxy}-benzamide,
5-{3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid, methyl ester,
N-{3-[3-(5-furan-2-yl-thieno[2,3d]pyrimidin-4-yloxy)-propoxy]- phenyl } acetamide,
2-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)benzamide,
5-{3-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yloxy]-propoxy}-nicotinic acid,
N-(3-{3-[(5-phenylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}phenyl)acetamide,
N-[4-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- hydrazinecarboxamide,
5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)pyridine-2- carboxamide,
5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide
N-methyl-5-{3-[(5-pyridin-4-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- pyridine-2-carboxamide,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-N- methylpyridine-2-carboxamide,
N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- pyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- pyridine-2-carboxamide.
60. At least one chemical entity of claim 1 wherein the compound of Formula I is chosen from
N-methyl-3-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4- yl)oxy]propoxy}benzamide,
N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)-thieno[2,3-d]pyrimidin-4-yl]amino}- propoxy)pyridine-2-carboxamide,
5-(3-{[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)-N- methylpyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4- yl)amino]propoxy}pyridine-2-carboxamide,
N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4- yl)amino]propoxy}pyridine-2-carboxamide, N-methyl-5-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}- nicotinamide,
5-{3-[(5-pyridin-3-ylthieno[2,3-d]-pyrimidin-4-yl)oxy]propoxy}pyridine-2- carboxamide hydroformate,
5-(4-fluorophenyl)-4-(3-{[l-methyl-5-(trifluoromethyl)-lH-pyrazol-3- yl]oxy}proρoxy)thieno[2,3-d]pyrimidine,
4-[3-(4-chlorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine,
4-[3-(3,5-difluorophenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine,
4-[3-(3,4-dimethoxyphenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3- djpyrimidine,
4-[3-(l,2-benzisothiazol-3-yloxy)propoxy]-5-(4-fluorophenyl)thieno[2,3- d]pyrimidine,
5-(4-fluorophenyl)-4-(3-phenoxypropoxy)-thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-[3-(3-methoxy-phenoxy)propoxy]thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-[3-(3-methyl-phenoxy)propoxy]thieno[2,3-d]pyrimidine, 5-(4-fluorophenyl)-4-{3-[3-(trifluoro-methoxy)phenoxy]propoxy}thieno[2,3- djpyrimidine,
4-[3-(3-ethylphenoxy)propoxy]-5-(4-fluorophenyl)thieno[2,3-d]pyrimidine, 5-{3-[(5-pyrimidin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine-
2-carboxylic acid hydroformate,
5-{3-[(5-pyrazin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxylic acid hydroformate,
4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butan- 1 -ol,
4-but-3-en-l-yl-5-(4-fluorophenyl)-thieno[2,3-d]pyrimidine,
Methyl 5-{4-[5-(4-fluorophenyl)-thieno[2,3-d]pyrimidin-4-yl]butoxy}-nicotinate,
5- {4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinic acid hydroformate,
5-{4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy}-N- methylnicotinamide hydroformate, 5- {4-[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]butoxy} nicotinamide hydroformate,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}phenoxy)- phenyl]acetamide hydroformate,
N-[3-(2,2-dimethyl-3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)phenyl]acetamide hydroformate,
N-[3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}phenoxy)phenyl]- acetamide hydroformate,
N-[3-(3-{[5-(4-fluorophenyl)thieno[2,3-d]ρyrimidin-4-yl]oxy}-2,2-dimethyl- propoxy)phenyl] acetamide hydroformate,
5-(3-{[5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)-l,3-dihydro- 2H-indol-2-one,
5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}pyridine-2- carboxamide hydroformate,
5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)pyridine- 2-carboxamide hydroformate,
N- [4-(3 - { [ 5 -(4-fluorophenyl)thieno [2 ,3 -d]pyrimidin-4-yl] amino } propyl)phenyl] - acetamide,
N-(3-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] acetamide,
N-(3-{3-[(5-pyridin-2-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[4-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] acetamide,
N-(4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)amino]propoxy}phenyl)- acetamide,
N-[4-(3-{[5-(5-chloro-2-furyl)thieno[2,3-d]pyrimidin-4-yl]amino}propoxy)- phenyl] acetamide,
5-(4-fluorophenyl)-4-[3-(3-nitrophenoxy)propoxy]thieno[2,3-d]pyrimidine, N-methyl-4-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- benzamide,
N-methyl-5-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- nicotinamide,
N-methyl-5-{3-[(5-pyridin-3-ylthieno[2,3-d]pyrimidin-4-yl)oxy]propoxy}- nicotinamide,
N-methyl-3-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)- benzamide,
N-[4-(3-{[5-(l,3-thiazol-2-yl)thieno[2,3-d]pyrimidin-4-yl]oxy}propoxy)phenyl]- acetamide, and
N-(4- {3 - [(5-pyridin-3 -ylthieno [2,3 -d]pyrimidin-4-yl)oxy]propoxy } phenyl)- acetamide.
61. The compound N-[3-(3- {[5-(4-fiuorophenyl)thieno[2,3-d]pyrimidin-4- yl]oxy}propoxy)-phenyl]acetamide.
62. A pharmaceutical composition comprising at least one chemical entity of any one of claims 1 to 61 and a pharmaceutically acceptable carrier.
63. A method of selectively inhibiting PDElO enzyme in a patient in need thereof comprising administering to said patient an effective amount of at least one chemical entity of any one of claims 1 to 61.
64. A method according to claim 63, wherein said patient is suffering from a psychiatric or neurological syndrome.
65. A method according to claim 63, wherein said method enhances cognition in said patient.
66. A method according to claim 63, wherein said method treats cognition impairment or decline in said patient
67. A method according to claim 63, wherein said patient is suffering from psychoses.
68. A method according to claim 67, wherein said psychoses is schizophrenia or bipolar disorder.
69. A method according to claim 63, wherein said patient is suffering from obsessive- compulsive disorder.
70. A method according to claim 63, wherein said patient is suffering from Parkinson's disease.
71. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with Parkinson's disease.
72. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with Alzheimer's disease.
73. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with dementia.
74. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with epilepsy.
75. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with multiple sclerosis.
76. A method according to claim 63, wherein said patient is suffering from memory and/or cognitive impairment associated with Huntington's disease.
77. A method according to claim 63, wherein said patient is suffering from a disorder affecting the function of the basal ganglia.
78. A method according to claim 63, wherein said patient is suffering from diabetes.
79. A method according to claim 63, wherein said patient is suffering from obesity.
80. The use of at least one chemical entity for the manufacture of a medicament for the treatment of a patient having a disease responsive to inhibition of PDElO enzyme, wherein the at least one chemical entity is a chemical entity of any one of claims 1 to 61.
81. A method for the manufacture of a medicament for the treatment of a patient having a disease responsive to inhibition of PDElO enzyme, comprising including in said medicament at least one chemical entity of any one of claims 1 to 61.
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