WO2006066001A3 - Immobilized degradable cationic polymer for transfecting eukaryotic cells - Google Patents

Immobilized degradable cationic polymer for transfecting eukaryotic cells Download PDF

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Publication number
WO2006066001A3
WO2006066001A3 PCT/US2005/045429 US2005045429W WO2006066001A3 WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3 US 2005045429 W US2005045429 W US 2005045429W WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
immobilized
culture device
eukaryotic cells
cationic polymer
Prior art date
Application number
PCT/US2005/045429
Other languages
French (fr)
Other versions
WO2006066001A2 (en
Inventor
Yasunobu Tanaka
Chris P Castello
Lei Yu
Original Assignee
Nitto Denko Corp
Yasunobu Tanaka
Chris P Castello
Lei Yu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Denko Corp, Yasunobu Tanaka, Chris P Castello, Lei Yu filed Critical Nitto Denko Corp
Priority to CA002590820A priority Critical patent/CA2590820A1/en
Priority to KR1020137000063A priority patent/KR20130010036A/en
Priority to EP05854197A priority patent/EP1836293A2/en
Priority to AU2005316501A priority patent/AU2005316501B2/en
Priority to JP2007546895A priority patent/JP4739352B2/en
Publication of WO2006066001A2 publication Critical patent/WO2006066001A2/en
Publication of WO2006066001A3 publication Critical patent/WO2006066001A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A cell transfection/culture device is disclosed which includes a solid support coated with a degradable polymer cation as a transfection reagent. The transfection/culture device is conveniently stored at room temperature until use. Cell transfection is accomplished easily by adding the nucleic acid of interest and the cells to be transfected to the transfection/culture device. Cell transfection is completed in less than one hour by using the transfection/culture device described herein.
PCT/US2005/045429 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells WO2006066001A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002590820A CA2590820A1 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells
KR1020137000063A KR20130010036A (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells
EP05854197A EP1836293A2 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells
AU2005316501A AU2005316501B2 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells
JP2007546895A JP4739352B2 (en) 2004-12-17 2005-12-14 Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17

Publications (2)

Publication Number Publication Date
WO2006066001A2 WO2006066001A2 (en) 2006-06-22
WO2006066001A3 true WO2006066001A3 (en) 2006-09-08

Family

ID=36353906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/045429 WO2006066001A2 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells

Country Status (7)

Country Link
EP (1) EP1836293A2 (en)
JP (1) JP4739352B2 (en)
KR (2) KR101263173B1 (en)
CN (1) CN101124316A (en)
AU (1) AU2005316501B2 (en)
CA (1) CA2590820A1 (en)
WO (1) WO2006066001A2 (en)

Cited By (18)

* Cited by examiner, † Cited by third party
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US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
USD1029291S1 (en) 2020-12-14 2024-05-28 Becton, Dickinson And Company Single piece reagent holder

Families Citing this family (23)

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US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP3718635A1 (en) 2003-07-31 2020-10-07 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
BR112013026451B1 (en) 2011-04-15 2021-02-09 Becton, Dickinson And Company system and method to perform molecular diagnostic tests on several samples in parallel and simultaneously amplification in real time in plurality of amplification reaction chambers
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
BR112014018995B1 (en) 2012-02-03 2021-01-19 Becton, Dickson And Company systems to perform automated testing
CN102827756B (en) * 2012-06-25 2014-04-09 华南农业大学 Agrobacterium local invasion method applied in soybean hypocotyl explant transformation system, and device thereof
CN105579584B (en) * 2013-06-28 2020-08-28 埃泽瑞斯公司 Compositions for introducing RNA into cells
US20180214572A1 (en) * 2014-11-10 2018-08-02 Ethris Gmbh Induction of osteogenesis by delivering bmp encoding rna
PT3523431T (en) * 2016-10-05 2021-01-04 Syngenta Participations Ag Improvements in or relating to gene silencing
CN109735573A (en) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 A kind of transient transfection reagent and its application method
EP3798250A1 (en) * 2019-09-25 2021-03-31 University College Dublin Hyperbranched cationic polymers useful as nucleic acid delivery vectors for transfecting
CN112195193A (en) * 2020-09-30 2021-01-08 上海交通大学 Application of single-stranded DNA in gene transfection
CN114904003B (en) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 Use of ionizable cationic lipid analog materials as nucleic acid drug delivery vehicles or transfection reagents

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (en) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection of nucleic acid
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (en) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Methods for transfecting cells using dna minor groove binding agents
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

Family Cites Families (6)

* Cited by examiner, † Cited by third party
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US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
WO2001020015A1 (en) * 1999-09-17 2001-03-22 Whitehead Institute For Biomedical Research Reverse transfection method
CA2427916C (en) * 2000-11-03 2012-01-17 The Regents Of The University Of Michigan Surface transfection and expression procedure
EP1513538A4 (en) * 2002-06-14 2007-08-22 Mirus Bio Corp Novel methods for the delivery of polynucleotides to cells
US6878374B2 (en) * 2003-02-25 2005-04-12 Nitto Denko Corporation Biodegradable polyacetals
CN100487122C (en) * 2004-02-18 2009-05-13 日东电工株式会社 Culture device and method for eukaryotic cell transfection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (en) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection of nucleic acid
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (en) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Methods for transfecting cells using dna minor groove binding agents
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9458487B2 (en) 2002-10-08 2016-10-04 Pfenex, Inc. Expression of mammalian proteins in pseudomonas fluorescens
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
USD1029291S1 (en) 2020-12-14 2024-05-28 Becton, Dickinson And Company Single piece reagent holder

Also Published As

Publication number Publication date
EP1836293A2 (en) 2007-09-26
KR101263173B1 (en) 2013-05-13
KR20070101859A (en) 2007-10-17
KR20130010036A (en) 2013-01-24
JP4739352B2 (en) 2011-08-03
CA2590820A1 (en) 2006-06-22
AU2005316501B2 (en) 2010-10-21
CN101124316A (en) 2008-02-13
WO2006066001A2 (en) 2006-06-22
AU2005316501A1 (en) 2006-06-22
JP2008523810A (en) 2008-07-10

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