WO2006061851A3 - Method for production of a therapeutically pure granulocyte macrophage - colony stimulating factor - Google Patents

Method for production of a therapeutically pure granulocyte macrophage - colony stimulating factor Download PDF

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Publication number
WO2006061851A3
WO2006061851A3 PCT/IN2005/000088 IN2005000088W WO2006061851A3 WO 2006061851 A3 WO2006061851 A3 WO 2006061851A3 IN 2005000088 W IN2005000088 W IN 2005000088W WO 2006061851 A3 WO2006061851 A3 WO 2006061851A3
Authority
WO
WIPO (PCT)
Prior art keywords
csf
protein
production
stimulating factor
colony stimulating
Prior art date
Application number
PCT/IN2005/000088
Other languages
French (fr)
Other versions
WO2006061851A2 (en
Inventor
Uma Devi Komath
Mitali Samaddar
Sanjay Lodha
Venkateswar Rao Hazari
Shreeram Nallar Chakravarthy
Lalitha Movva
Jayaram Chigurupati
Original Assignee
Zenotech Lab Ltd
Uma Devi Komath
Mitali Samaddar
Sanjay Lodha
Venkateswar Rao Hazari
Shreeram Nallar Chakravarthy
Lalitha Movva
Jayaram Chigurupati
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zenotech Lab Ltd, Uma Devi Komath, Mitali Samaddar, Sanjay Lodha, Venkateswar Rao Hazari, Shreeram Nallar Chakravarthy, Lalitha Movva, Jayaram Chigurupati filed Critical Zenotech Lab Ltd
Priority to AP2007004049A priority Critical patent/AP2007004049A0/en
Priority to EP05732861A priority patent/EP1833846A2/en
Publication of WO2006061851A2 publication Critical patent/WO2006061851A2/en
Publication of WO2006061851A3 publication Critical patent/WO2006061851A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A simple and scaleable process is provided for obtaining recombinant human GM CSF expressed in microbial cells, secreting mature GM-CSF, culturing these cells and purifying the protein in good yield and of a therapeutic grade quality. The steps include cloning, fermentation and purification of the protein. The purified protein is got by a process that involves lysing the microorganism, extraction of the processed and unprocessed forms of GM-CSF from the membrane pellet and selectively purifying the processed form of the protein from the unprocessed form by a two-step chromatography procedure. The purity of the processed form of GM-CSF (without signal peptide) obtained has endotoxin units, host cell proteins and host cell DNA within the specified compendial limits for therapeutic proteins.
PCT/IN2005/000088 2004-12-09 2005-03-18 Method for production of a therapeutically pure granulocyte macrophage - colony stimulating factor WO2006061851A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AP2007004049A AP2007004049A0 (en) 2004-12-09 2005-03-18 Method for production of therapeutically pure granulocyte macrophage-colony stimulating factor
EP05732861A EP1833846A2 (en) 2004-12-09 2005-03-18 Method for production of a therapeutically pure granulocyte macrophage - colony stimulating factor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1343CH2004 2004-12-09
IN1343/CHE/2004 2004-12-09

Publications (2)

Publication Number Publication Date
WO2006061851A2 WO2006061851A2 (en) 2006-06-15
WO2006061851A3 true WO2006061851A3 (en) 2009-04-23

Family

ID=36578319

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2005/000088 WO2006061851A2 (en) 2004-12-09 2005-03-18 Method for production of a therapeutically pure granulocyte macrophage - colony stimulating factor

Country Status (3)

Country Link
EP (1) EP1833846A2 (en)
AP (1) AP2007004049A0 (en)
WO (1) WO2006061851A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020234742A1 (en) * 2019-05-20 2020-11-26 Lupin Limited Granulocyte colony stimulating factor purification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BORJALILOO, S. ET AL.: "Comparison of T7- and lac-based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Fator in Escherichia coli", IRANIAN J. BIOTECHNOL., vol. 1, no. 2, April 2003 (2003-04-01), pages 101 - 108, XP008119100 *
HOLOWACHUK, E.W. ET AL.: "Biologically Active Recombinant Rat Granulocyte Macrophage Colony-stimulating Factor Produced in Escherichia coli", PROTEIN EXPR. PURIF., vol. 6, no. 5, 1 October 1995 (1995-10-01), pages 588 - 596, XP002273147 *
NICOLA, N.A. ET AL.: "Purification of a Factor Inducing Differentiation in Murine Myelomonocytic Leukemia Cells. Identification as Granulocyte Colony-stimulating Factor", J. BIOL. CHEM., vol. 258, no. 14, 25 July 1983 (1983-07-25), pages 9017 - 9023, XP008118769 *

Also Published As

Publication number Publication date
EP1833846A2 (en) 2007-09-19
AP2007004049A0 (en) 2007-06-30
WO2006061851A2 (en) 2006-06-15

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