WO2006058942A3 - Method of producing 6-aminopenicillanic acid using the aculeacin a acylase enzyme of actinoplanes utahensis, which is purified from recombinant bacteria streptomyces lividans cect 3377 - Google Patents

Method of producing 6-aminopenicillanic acid using the aculeacin a acylase enzyme of actinoplanes utahensis, which is purified from recombinant bacteria streptomyces lividans cect 3377 Download PDF

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Publication number
WO2006058942A3
WO2006058942A3 PCT/ES2005/000643 ES2005000643W WO2006058942A3 WO 2006058942 A3 WO2006058942 A3 WO 2006058942A3 ES 2005000643 W ES2005000643 W ES 2005000643W WO 2006058942 A3 WO2006058942 A3 WO 2006058942A3
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WO
WIPO (PCT)
Prior art keywords
aculeacin
cect
purified
producing
streptomyces lividans
Prior art date
Application number
PCT/ES2005/000643
Other languages
Spanish (es)
French (fr)
Other versions
WO2006058942A2 (en
WO2006058942B1 (en
Inventor
La Mata Riesco Isabel De
Bacete Jesus Torres
Cisneros Daniel Hormigo
Leon Maribel Stuart
Sanchez Miguel Arroyo
Original Assignee
Univ Complutense De Madrid Rec
La Mata Riesco Isabel De
Bacete Jesus Torres
Cisneros Daniel Hormigo
Leon Maribel Stuart
Sanchez Miguel Arroyo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Complutense De Madrid Rec, La Mata Riesco Isabel De, Bacete Jesus Torres, Cisneros Daniel Hormigo, Leon Maribel Stuart, Sanchez Miguel Arroyo filed Critical Univ Complutense De Madrid Rec
Publication of WO2006058942A2 publication Critical patent/WO2006058942A2/en
Publication of WO2006058942A3 publication Critical patent/WO2006058942A3/en
Publication of WO2006058942B1 publication Critical patent/WO2006058942B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position

Abstract

The invention relates to a method of producing 6-aminopenicillanic acid using the aculeacin A acylase enzyme of Actinoplanes utahensis, which is purified from recombinant bacteria Streptomyces lividans CECT 3377. More specifically, the invention relates to a method of producing 6-aminopenicillanic acid, comprising the hydrolysis of penicillins V, K, F or dihydroF, using the aculeacin A acylase enzyme of bacteria Actinoplanes utahensis which is purified from broth cultures of cells of recombinant bacteria Streptomyces lividans CECT 3367 which carry the aac gene that encodes said enzyme.
PCT/ES2005/000643 2004-11-26 2005-11-25 Method of producing 6-aminopenicillanic acid using the aculeacin a acylase enzyme of actinoplanes utahensis, which is purified from recombinant bacteria streptomyces lividans cect 3377 WO2006058942A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200402857A ES2304191B2 (en) 2004-11-26 2004-11-26 PROCEDURE TO PRODUCE ACID-6-AMINOPENICILANIC THROUGH THE ACILASA ACILASA ACILASA OF ACTINOPLANES UTAHENSIS, PURIFIED FROM STREPTOMYCES LIVIDANS RECOMMENDING BACTERIA CECT 3377.
ESP200402857 2004-11-26

Publications (3)

Publication Number Publication Date
WO2006058942A2 WO2006058942A2 (en) 2006-06-08
WO2006058942A3 true WO2006058942A3 (en) 2006-08-24
WO2006058942B1 WO2006058942B1 (en) 2006-09-21

Family

ID=36565395

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2005/000643 WO2006058942A2 (en) 2004-11-26 2005-11-25 Method of producing 6-aminopenicillanic acid using the aculeacin a acylase enzyme of actinoplanes utahensis, which is purified from recombinant bacteria streptomyces lividans cect 3377

Country Status (2)

Country Link
ES (1) ES2304191B2 (en)
WO (1) WO2006058942A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2105989A1 (en) * 1996-03-15 1997-10-16 Antibioticos Sa Alternative process for producing 6-amino-penicillanic acid (6-apa)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2105989A1 (en) * 1996-03-15 1997-10-16 Antibioticos Sa Alternative process for producing 6-amino-penicillanic acid (6-apa)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
INOKOSHI J. ET AL.: "Efficient production of aculeacin-A acylase in recombinant streptomyces strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 39, no. 4-5, July 1993 (1993-07-01), pages 532 - 536, XP001095451 *
INOKOSHI J. ET AL.: "Identification of precursor peptide of aculeacin A acylase as a protein with proteolytic activity", FEMS MICROBIOLOGY LETTERS, vol. 114, no. 3, 15 December 1993 (1993-12-15), pages 305 - 309 *
KREUZMAN A.J. ET AL.: "Membrane-associated echinocndin B deacylase of Actinoplanes utahensis: purification, characterization, heterologous cloning and enzymatic deacylation reaction", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 24, no. 3, March 2000 (2000-03-01), pages 173 - 180 *
PARMAR A. ET AL.: "Advances in enzymatic transformation of penicillins to 6-aminopenicillanic acid (6-APA)", BIOTECHNOLOGY ADVANCES, vol. 18, 2000, pages 289 - 301, XP004213782 *

Also Published As

Publication number Publication date
ES2304191B2 (en) 2009-10-29
WO2006058942A2 (en) 2006-06-08
WO2006058942B1 (en) 2006-09-21
ES2304191A1 (en) 2008-09-16

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