WO2006058226A3 - Modified dimeric streptavidins and uses thereof - Google Patents

Modified dimeric streptavidins and uses thereof Download PDF

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Publication number
WO2006058226A3
WO2006058226A3 PCT/US2005/042766 US2005042766W WO2006058226A3 WO 2006058226 A3 WO2006058226 A3 WO 2006058226A3 US 2005042766 W US2005042766 W US 2005042766W WO 2006058226 A3 WO2006058226 A3 WO 2006058226A3
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WO
WIPO (PCT)
Prior art keywords
streptavidin
chain
molecules
proteins
biotin
Prior art date
Application number
PCT/US2005/042766
Other languages
French (fr)
Other versions
WO2006058226A2 (en
WO2006058226A9 (en
Inventor
Charles R Cantor
Filiz M Aslan
Original Assignee
Univ Boston
Charles R Cantor
Filiz M Aslan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Boston, Charles R Cantor, Filiz M Aslan filed Critical Univ Boston
Publication of WO2006058226A2 publication Critical patent/WO2006058226A2/en
Publication of WO2006058226A9 publication Critical patent/WO2006058226A9/en
Publication of WO2006058226A3 publication Critical patent/WO2006058226A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention provides novel modified streptavidin proteins, nucleic acids encoding such proteins, and uses of such proteins. Specifically, the invention provides single-chain dimeric streptavidin molecules. The invention also provides modified single-chain streptavidins with altered affinity to biotin and biotin derivatives, such as biotin-4-fluorescein. The modified single-chain dimeric streptavidin proteins can be used in analysis or composite separation either alone or in combination with ordinary streptavidin-biotin systems to visualize and/or separate composites and molecules. The single-chain streptavidin dimers of the invention also allow creating of more stable streptavidin tetramers. The single-chain streptavidin dimers of the invention can further be used as smaller sized functional streptavidin molecules in various in vivo methods, where the size of the conventional tetrameric streptavidin makes the use of such tetramers difficult or impossible. Such in vivo uses include diagnostic imaging and targeting of therapeutic molecules into individuals. The invention further provides methods of preparing and identifying novel modified single-chain streptavidin molecules.
PCT/US2005/042766 2004-11-24 2005-11-23 Modified dimeric streptavidins and uses thereof WO2006058226A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US63089904P 2004-11-24 2004-11-24
US60/630,899 2004-11-24
US64308805P 2005-01-11 2005-01-11
US60/643,088 2005-01-11

Publications (3)

Publication Number Publication Date
WO2006058226A2 WO2006058226A2 (en) 2006-06-01
WO2006058226A9 WO2006058226A9 (en) 2006-07-13
WO2006058226A3 true WO2006058226A3 (en) 2006-08-24

Family

ID=36225542

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/042766 WO2006058226A2 (en) 2004-11-24 2005-11-23 Modified dimeric streptavidins and uses thereof

Country Status (1)

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WO (1) WO2006058226A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993714B2 (en) 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
WO2009055068A1 (en) * 2007-10-26 2009-04-30 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
DE102008053892A1 (en) 2008-10-30 2010-05-06 Fachhochschule Gelsenkirchen Medical implant with biofunctionalized surface
WO2010132363A1 (en) * 2009-05-11 2010-11-18 Imiplex Llc Method of protein nanostructure fabrication
EP2400304A1 (en) 2010-06-22 2011-12-28 Centro de Investigación Cooperativa En Biomateriales ( CIC biomaGUNE) Method for the characterization of intermolecular interactions
WO2012123269A1 (en) 2011-03-11 2012-09-20 Proyecto De Biomedicina Cima, S.L. Immunogenic compositions and methods for their use
EP2505640A1 (en) 2011-03-29 2012-10-03 Neo Virnatech, S.L. Vaccine compositions for birnavirus-borne diseases
EP2743695A1 (en) 2012-12-12 2014-06-18 Nanogap Sub NM Powder, S.A. Methods and reagents for the detection of biomolecules using luminescence
WO2015101666A1 (en) 2014-01-03 2015-07-09 Fundación Biofísica Bizkaia VLPs, METHODS FOR THEIR OBTENTION AND APPLICATIONS THEREOF
CN116656605A (en) * 2014-04-16 2023-08-29 朱诺治疗有限公司 Methods, kits and devices for expanding cell populations
MA45488A (en) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh CELL CULTURE PROCESSES, KITS AND APPARATUS
MA45489A (en) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh CELL CULTURE PROCESSES, ASSOCIATED KITS AND APPARATUS
RU2021134624A (en) 2015-10-22 2022-03-15 Джуно Терапьютикс Гмбх METHODS, KITS, MEANS AND DEVICES FOR TRANSDUCTION
WO2018162450A1 (en) 2017-03-06 2018-09-13 Fundación Para La Investigación Médica Aplicada New inmunostimulatory compositions comprising an entity of cold inducible rna-binding protein with an antigen for the activation of dendritic cells
CA3060526A1 (en) 2017-04-27 2018-11-01 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
CN116348498A (en) 2020-10-16 2023-06-27 希森美康株式会社 Method and kit for determining polypeptide, polymer, solid phase and test substance
JP2023132075A (en) 2022-03-10 2023-09-22 シスメックス株式会社 L-biotin measurement reagent, method of measuring l-biotin-containing sample, method of determining number of labels of l-biotin-labeled substance, and method of producing solid phase consisting of immobilized optically isomeric avidins
EP4371569A1 (en) 2022-11-16 2024-05-22 Universidad del País Vasco/Euskal Herriko Unibertsitatea Vlps against acute myeloid leukaemia

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051632A1 (en) * 1998-04-03 1999-10-14 University Of Washington Circularly permuted biotin binding proteins
WO2001005977A1 (en) * 1999-07-15 2001-01-25 Yeda Research And Development Co. Ltd. Generation of stable dimers with reversible biotin-binding activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051632A1 (en) * 1998-04-03 1999-10-14 University Of Washington Circularly permuted biotin binding proteins
WO2001005977A1 (en) * 1999-07-15 2001-01-25 Yeda Research And Development Co. Ltd. Generation of stable dimers with reversible biotin-binding activity

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ASLAN FILIZ M ET AL: "Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 14 JUN 2005, vol. 102, no. 24, 14 June 2005 (2005-06-14), pages 8507 - 8512, XP002383753, ISSN: 0027-8424 *
CHILKOTI A ET AL: "ENGINEERED CHIMERIC STREPTAVIDIN TETRAMERS AS NOVEL TOOLS FOR BIOSEPARATIONS AND DRUG DELIVERY", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 13, November 1995 (1995-11-01), pages 1198 - 1204, XP000961183, ISSN: 0733-222X *
CHU V ET AL: "Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, CAMBRIDGE, GB, vol. 7, no. 4, April 1998 (1998-04-01), pages 848 - 859, XP002316238, ISSN: 0961-8368 *
NORDLUND H R ET AL: "Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 279, no. 35, 27 August 2004 (2004-08-27), pages 36715 - 36719, XP002316240, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2006058226A2 (en) 2006-06-01
WO2006058226A9 (en) 2006-07-13

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