WO2006055040A3 - Identification de proteines dans un genome - Google Patents

Identification de proteines dans un genome Download PDF

Info

Publication number
WO2006055040A3
WO2006055040A3 PCT/US2005/019477 US2005019477W WO2006055040A3 WO 2006055040 A3 WO2006055040 A3 WO 2006055040A3 US 2005019477 W US2005019477 W US 2005019477W WO 2006055040 A3 WO2006055040 A3 WO 2006055040A3
Authority
WO
WIPO (PCT)
Prior art keywords
provides
proteins
protein
site
transposon
Prior art date
Application number
PCT/US2005/019477
Other languages
English (en)
Other versions
WO2006055040A2 (fr
Inventor
James L Hartley
Dominic Esposito
Kelly Jeanne Stanard
Original Assignee
Us Gov Health & Human Serv
James L Hartley
Dominic Esposito
Kelly Jeanne Stanard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, James L Hartley, Dominic Esposito, Kelly Jeanne Stanard filed Critical Us Gov Health & Human Serv
Publication of WO2006055040A2 publication Critical patent/WO2006055040A2/fr
Publication of WO2006055040A3 publication Critical patent/WO2006055040A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Abstract

La présente invention concerne un procédé de production de protéines solubles. Dans un mode de réalisation, cette invention concerne un procédé de production de dérivés de délétion solubles d'une protéine comprenant l'intégration de transposon et le clonage de recombinaison au moyen de recombinases spécifiques de site. Dans un autre mode de réalisation, cette invention concerne un procédé d'identification de chacune des au moins deux protéines solubles, qui consiste à analyser simultanément des cassettes de lecture ouvertes groupées. Ces deux modes de réalisation comprennent la purification de protéines, la séparation de protéines et l'analyse spectroscopique de masse des protéines séparées. Cette invention concerne aussi un transposon comprenant un gène marqueur sélectionnable flanqué par des séquences de recombinaison spécifiques du site inversées identiques.
PCT/US2005/019477 2004-11-19 2005-06-03 Identification de proteines dans un genome WO2006055040A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62894804P 2004-11-19 2004-11-19
US60/628,948 2004-11-19

Publications (2)

Publication Number Publication Date
WO2006055040A2 WO2006055040A2 (fr) 2006-05-26
WO2006055040A3 true WO2006055040A3 (fr) 2006-08-24

Family

ID=34972198

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/019477 WO2006055040A2 (fr) 2004-11-19 2005-06-03 Identification de proteines dans un genome

Country Status (1)

Country Link
WO (1) WO2006055040A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9150881B2 (en) 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
WO2005062881A2 (fr) 2003-12-24 2005-07-14 Transgenrx, Inc. Therapie genique faisant intervenir des vecteurs de transposon

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BUESSOW KONRAD ET AL: "A human cDNA library for high-throughput protein expression screening", GENOMICS, vol. 65, no. 1, 1 April 2000 (2000-04-01), pages 1 - 8, XP004439385, ISSN: 0888-7543 *
CHATTERJEE PRADEEP K ET AL: "Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs", NUCLEIC ACIDS RESEARCH, vol. 32, no. 18, 2004, pages 5668 - 5676, XP002380388, ISSN: 0305-1048 *
HONORÉ BENT ET AL: "Functional genomics studied by proteomics.", BIOESSAYS : NEWS AND REVIEWS IN MOLECULAR, CELLULAR AND DEVELOPMENTAL BIOLOGY. AUG 2004, vol. 26, no. 8, August 2004 (2004-08-01), pages 901 - 915, XP009053681, ISSN: 0265-9247 *
WALHOUT A J M ET AL: "GATEWAY RECOMBINATIONAL CLONING: APPLICATION TO THE CLONING OF LARGE NUMBERS OF OPEN READING FRAMES OR ORFEOMES", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 328, 2000, pages 575 - 592, XP001056139, ISSN: 0076-6879 *
ZHANG SEN ET AL: "Resources for targeted insertional and deletional mutagenesis in Arabidopsis.", PLANT MOLECULAR BIOLOGY, vol. 53, no. 1-2, September 2003 (2003-09-01), pages 133 - 150, XP002380387, ISSN: 0167-4412 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
US9150881B2 (en) 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors

Also Published As

Publication number Publication date
WO2006055040A2 (fr) 2006-05-26

Similar Documents

Publication Publication Date Title
WO2006031800A3 (fr) Transfection inverse de reseaux de cellules pour des analyses structurelles et fonctionnelles de proteines
WO2007037678A3 (fr) Criblage a haut debit de populations mutagenisees
WO2007065025A3 (fr) Procede d'analyse d'adn au moyen de micro/nano-canaux
WO2004081174A3 (fr) Marqueur pour detecter une cellule souche mesenchymateuse et procede pour distinguer une cellule souche mesenchymateuse au moyen d'un marqueur
HK1072957A1 (en) In vitro peptide expression libraray
WO2005047511A3 (fr) Expression bacterienne d'inhibiteurs de protease et variants de ceux-ci
WO2008069906A3 (fr) Expression numérisée de l'analyse génétique
AU2002348563A1 (en) Nucleic acid enrichment
WO2005007814A3 (fr) Mappage genomique d'elements d'adn fonctionnels et de proteines cellulaires
WO2006044728A3 (fr) Procedes d'isolation d'acides nucleiques de spores procaryotiques
WO2005005651A3 (fr) Sequence de recepteur de lymphocyte t et methode destinee a la detection et au traitement de la polyarthrite rhumatoide
IL176479A0 (en) Methods and compositions for identifying rna-binding proteins
WO2006055040A3 (fr) Identification de proteines dans un genome
EA200600579A1 (ru) Способ получения плазмидной днк фармацевтического качества
WO2007050095A3 (fr) Vaccins améliorés et leurs procédés d'utilisation
WO2005116213A3 (fr) Evolution dirigee de proteines
WO2005067648A3 (fr) Profilage spatial multiplex d'expression genique
WO2007076143A3 (fr) Ensembles d'amorces specifiques d'especes et identification de sequences d'adn specifiques d'especes au moyen de l'enrichissement de fragments genomiques
WO2003022868A3 (fr) Proteines pouvant se lier avec des metaux et procedes associes
WO2005018552A3 (fr) Proteines liant l'heparine : capteurs pour la detection de l'heparine
EP1847611A3 (fr) Systèmes d'expression pouvant être induits par le rhamnose
WO2007050735A8 (fr) Marqueurs de traits de production
WO2006028565A3 (fr) Procedes pour analyse de site haut rendement au niveau du genome
WO2003093434A3 (fr) Genome nanoarchaeum, polypeptides nanoarchaeum et acides nucleiques les codant et leurs procedes de fabrication et d'utilisation
WO2006097336A3 (fr) Nouveau composant du mecanisme de signalisation wg/wnt

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 05758485

Country of ref document: EP

Kind code of ref document: A2