WO2006054262A3 - Use of phage display technique for identifying peptides capable of binding progenitor/stem cells, peptides thereby obtained and uses thereof - Google Patents

Use of phage display technique for identifying peptides capable of binding progenitor/stem cells, peptides thereby obtained and uses thereof Download PDF

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Publication number
WO2006054262A3
WO2006054262A3 PCT/IB2005/053811 IB2005053811W WO2006054262A3 WO 2006054262 A3 WO2006054262 A3 WO 2006054262A3 IB 2005053811 W IB2005053811 W IB 2005053811W WO 2006054262 A3 WO2006054262 A3 WO 2006054262A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptides
stem cells
phage display
display technique
progenitor
Prior art date
Application number
PCT/IB2005/053811
Other languages
French (fr)
Other versions
WO2006054262A2 (en
Inventor
Roberto Perris
Nardo Paolo Di
Original Assignee
Univ Roma
Roberto Perris
Nardo Paolo Di
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Roma, Roberto Perris, Nardo Paolo Di filed Critical Univ Roma
Publication of WO2006054262A2 publication Critical patent/WO2006054262A2/en
Publication of WO2006054262A3 publication Critical patent/WO2006054262A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present application relates to the utilization of phage display technique, based on phage libraries of random sequence type or with defined amino acid sequences for identifying peptide sequences capable of binding human progenitor/stem cells, as well as peptides and derivatives thereof identified and isolated by such methods. The present invention further relates to methods of recognizing, monitoring and modifying progenitor/stem cells by use of complexes comprising said peptides and derivatives .
PCT/IB2005/053811 2004-11-18 2005-11-18 Use of phage display technique for identifying peptides capable of binding progenitor/stem cells, peptides thereby obtained and uses thereof WO2006054262A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2004A000568 2004-11-18
ITRM20040568 ITRM20040568A1 (en) 2004-11-18 2004-11-18 USE OF THE "PHAGE DISPLAY" TECHNIQUE FOR THE IDENTIFICATION OF PEPTIDES WITH CAPACITY OF STAMIN CELLS / PROGENITOR, PEPTIDES SO OBTAINED AND THEIR USES.

Publications (2)

Publication Number Publication Date
WO2006054262A2 WO2006054262A2 (en) 2006-05-26
WO2006054262A3 true WO2006054262A3 (en) 2006-10-05

Family

ID=36407529

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/053811 WO2006054262A2 (en) 2004-11-18 2005-11-18 Use of phage display technique for identifying peptides capable of binding progenitor/stem cells, peptides thereby obtained and uses thereof

Country Status (2)

Country Link
IT (1) ITRM20040568A1 (en)
WO (1) WO2006054262A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528109B2 (en) 2007-10-26 2016-12-27 Biomarin Technologies B.V. Methods and means for efficient skipping of exon 45 in duchenne muscular dystrophy pre-mRNA

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2049664B1 (en) 2006-08-11 2011-09-14 Prosensa Technologies B.V. Single stranded oligonucleotides complementary to repetitive elements for treating DNA repeat instability associated genetic disorders
US8501905B2 (en) 2007-03-22 2013-08-06 The Regents Of The University Of California Synthetic cell platforms and methods of use thereof
EP1985708B1 (en) * 2007-04-27 2015-04-15 Universität Rostock Selective targeting of viruses to neural precursor cells
WO2009008725A2 (en) 2007-07-12 2009-01-15 Prosensa Technologies B.V. Molecules for targeting compounds to various selected organs, tissues or tumor cells
NZ582521A (en) 2007-07-12 2011-09-30 Prosensa Technologies Bv A conjugate comprising the amino acid sequence LGAQSNF for targeting compounds to muscle tissue
USRE48468E1 (en) 2007-10-26 2021-03-16 Biomarin Technologies B.V. Means and methods for counteracting muscle disorders
NZ587178A (en) 2008-02-08 2011-11-25 Prosensa Holding Bv Methods and means for treating dna repeat instability associated genetic disorders
EP2119783A1 (en) 2008-05-14 2009-11-18 Prosensa Technologies B.V. Method for efficient exon (44) skipping in Duchenne Muscular Dystrophy and associated means
US20110003702A1 (en) * 2009-06-03 2011-01-06 President And Fellows Of Harvard College Methods, systems, and kits for identification of osteoinductive peptides
WO2010123369A1 (en) 2009-04-24 2010-10-28 Prosensa Technologies B.V. Oligonucleotide comprising an inosine for treating dmd
WO2013009690A2 (en) 2011-07-09 2013-01-17 The Regents Of The University Of California Leukemia stem cell targeting ligands and methods of use
CN104203289B (en) 2012-01-27 2020-11-03 比奥马林技术公司 RNA-regulatory oligonucleotides with improved properties for the treatment of duchenne muscular dystrophy and becker muscular dystrophy
US9175263B2 (en) * 2012-08-22 2015-11-03 Biotime, Inc. Methods and compositions for targeting progenitor cell lines
CN113648323A (en) 2013-06-05 2021-11-16 再生疗法有限公司 Compositions and methods for induced tissue regeneration in mammalian species
CN103497236B (en) * 2013-09-25 2017-05-10 浙江省医学科学院 Specific heptapeptide targeting to WISP-1 protein and application thereof
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002020822A2 (en) * 2000-09-08 2002-03-14 Board Of Regents, The University Of Texas System Biopanning and rapid analysis of selective interactive ligands (brasil)
US20030219826A1 (en) * 1999-09-01 2003-11-27 Robbins Paul D. Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219826A1 (en) * 1999-09-01 2003-11-27 Robbins Paul D. Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses
WO2002020822A2 (en) * 2000-09-08 2002-03-14 Board Of Regents, The University Of Texas System Biopanning and rapid analysis of selective interactive ligands (brasil)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BARRY ET AL: "Towards cell-targeting gene therapy vectors: selection of cell-binding peptids from random peptide-presenting phage libraries", NATURE MEDICINE, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 2, no. 3, March 1996 (1996-03-01), pages 299 - 305, XP002086381, ISSN: 1078-8956 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528109B2 (en) 2007-10-26 2016-12-27 Biomarin Technologies B.V. Methods and means for efficient skipping of exon 45 in duchenne muscular dystrophy pre-mRNA

Also Published As

Publication number Publication date
ITRM20040568A1 (en) 2005-02-18
WO2006054262A2 (en) 2006-05-26

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