WO2006048465A3 - Animaux modèles comprenant au moins une mutation ou un ki et un ko conditionnel - Google Patents

Animaux modèles comprenant au moins une mutation ou un ki et un ko conditionnel Download PDF

Info

Publication number
WO2006048465A3
WO2006048465A3 PCT/EP2005/055826 EP2005055826W WO2006048465A3 WO 2006048465 A3 WO2006048465 A3 WO 2006048465A3 EP 2005055826 W EP2005055826 W EP 2005055826W WO 2006048465 A3 WO2006048465 A3 WO 2006048465A3
Authority
WO
WIPO (PCT)
Prior art keywords
conditional
mutation
animal models
target gene
knock
Prior art date
Application number
PCT/EP2005/055826
Other languages
English (en)
Other versions
WO2006048465A2 (fr
Inventor
Kader Thiam
Alexandre Fraichard
Original Assignee
Genoway
Kader Thiam
Alexandre Fraichard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genoway, Kader Thiam, Alexandre Fraichard filed Critical Genoway
Publication of WO2006048465A2 publication Critical patent/WO2006048465A2/fr
Publication of WO2006048465A3 publication Critical patent/WO2006048465A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une combinaison de séquences d'ADN permettant de développer des animaux modèles comprenant d'une part une modification de son génome afin d'introduire soit un nouveau gène pour être exprimé (KI ou Knock In) soit une mutation dans un gène cible et d'autre part un KO (Knock Out), constitutif ou conditionnel, dans un gène cible.
PCT/EP2005/055826 2004-11-08 2005-11-08 Animaux modèles comprenant au moins une mutation ou un ki et un ko conditionnel WO2006048465A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0411871 2004-11-08
FR0411871A FR2877673A1 (fr) 2004-11-08 2004-11-08 Animaux modeles comprenant au moins une mutation ou un ki et un ko conditionnel

Publications (2)

Publication Number Publication Date
WO2006048465A2 WO2006048465A2 (fr) 2006-05-11
WO2006048465A3 true WO2006048465A3 (fr) 2006-07-13

Family

ID=34952520

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/055826 WO2006048465A2 (fr) 2004-11-08 2005-11-08 Animaux modèles comprenant au moins une mutation ou un ki et un ko conditionnel

Country Status (2)

Country Link
FR (1) FR2877673A1 (fr)
WO (1) WO2006048465A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010149765A1 (fr) 2009-06-26 2010-12-29 Inserm (Institut National De La Sante Et De La Recherche Medicale) Modèles animaux non humains de l'augmentation de la perméabilité vasculaire rétinienne
US20120311728A1 (en) 2009-11-06 2012-12-06 Ziad Mallat Methods and pharmaceutical composition for the treatment of atherosclerosis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1116790A1 (fr) * 2000-01-14 2001-07-18 Institut National De La Sante Et De La Recherche Medicale (Inserm) Modele murin caracterise par la mutation conditionelle du gene smn - utilisation pour l'etude de l'atrophie musculaire spinale et d'autres maladies neurodégénératives et myopathies musculaires
US20030165497A1 (en) * 2000-07-19 2003-09-04 Lioubin Mario N. RRP sequences and knockout mice and uses thereof
US20040241851A1 (en) * 2003-05-30 2004-12-02 Wyeth Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
EP1483962A2 (fr) * 2003-06-06 2004-12-08 F. Hoffmann-La Roche Ag Modèle animal transgénique de la fonction de Glyt1

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1116790A1 (fr) * 2000-01-14 2001-07-18 Institut National De La Sante Et De La Recherche Medicale (Inserm) Modele murin caracterise par la mutation conditionelle du gene smn - utilisation pour l'etude de l'atrophie musculaire spinale et d'autres maladies neurodégénératives et myopathies musculaires
US20030165497A1 (en) * 2000-07-19 2003-09-04 Lioubin Mario N. RRP sequences and knockout mice and uses thereof
US20040241851A1 (en) * 2003-05-30 2004-12-02 Wyeth Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
EP1483962A2 (fr) * 2003-06-06 2004-12-08 F. Hoffmann-La Roche Ag Modèle animal transgénique de la fonction de Glyt1

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CASANOVA EMILIO ET AL: "Construction of a conditional allele of RSK-B/MSK2 in the mouse", GENESIS THE JOURNAL OF GENETICS AND DEVELOPMENT, vol. 32, no. 2, February 2002 (2002-02-01), pages 158 - 160, XP009048883, ISSN: 1526-954X *
LIU PENTAO ET AL: "A highly efficient recombineering-based method for generating conditional knockout mutations.", GENOME RESEARCH, vol. 13, no. 3, March 2003 (2003-03-01), pages 476 - 484, XP002376676, ISSN: 1088-9051 *
REN SHU-YUE ET AL: "A Hoxa2 mutant conditional allele generated by Flp- and Cre-mediated recombination", GENESIS THE JOURNAL OF GENETICS AND DEVELOPMENT, vol. 32, no. 2, February 2002 (2002-02-01), pages 105 - 108, XP009048884, ISSN: 1526-954X *

Also Published As

Publication number Publication date
WO2006048465A2 (fr) 2006-05-11
FR2877673A1 (fr) 2006-05-12

Similar Documents

Publication Publication Date Title
WO2007000668A3 (fr) Vecteur de genes
WO2008069906A3 (fr) Expression numérisée de l'analyse génétique
WO2007075967A3 (fr) Sequencage et genotypage au moyen de nucleotides de terminaison reversible
WO2005111217A3 (fr) Molecules d’acide nucleique de nicotiana et leurs utilisations
WO2007120533A3 (fr) Cassette d'expression comportant un minigène
WO2006046148A3 (fr) Constructions d'arn
EP1750771A4 (fr) Micro-arn d'ilot pancreatique et methodes permettant d'inhiber ce dernier
WO2007124493A3 (fr) PROCÉDÉS ET COMPOSITIONS PERMETTANT DE PRODUIRE DES PROTÉINES RECOMBINANTES EN UTILISANT UN GÈNE D'ARNt
WO2006043178A3 (fr) Enzymes et procedes
WO2007070280A3 (fr) Utilisation de polymorphismes de deletion pour predire, prevenir et controler l'histoincompatibilite
NZ595626A (en) Wood and cell wall gene microarray
WO2008085991A3 (fr) Chambre de réaction
WO2008082429A3 (fr) Eléments génétiques spécifiques d'une plante et cassettes de transfert pour une transformation de plante
HK1213015A1 (zh) 編碼 -甲基腐胺氧化酶之核酸及其應用
WO2004096842A3 (fr) Sequences de nucleotides et d'acides amines du virus du sras, et utilisations associees
WO2006128444A3 (fr) Proteine de resistance autoactivee
WO2005079367A3 (fr) Synthase d'acide gras (fas) schizochytrium et produits et procedes connexes
WO2007022374A3 (fr) Evaluation de la reponse a une therapie anti-cd21 par genotypage de composants c1q
WO2006048465A3 (fr) Animaux modèles comprenant au moins une mutation ou un ki et un ko conditionnel
WO2005035719A3 (fr) Luciferase modifiee
FR2850178B1 (fr) Clavier universel.
WO2006048466A3 (fr) Animaux modeles comprenant au moins un ko et un ki conditionnel
WO2008091283A3 (fr) Séquences nucléotidiques inhibitrices virales et vaccins
IL186142A0 (en) Gene sythesis using pooled dna
WO2006105277A3 (fr) Polynucleotides d'eg1117 et eg307 et leurs utilisations

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LC LK LR LS LT LU LV LY MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase

Ref document number: 05803453

Country of ref document: EP

Kind code of ref document: A2