WO2006045049A1 - Angiopoietin-2 specific binding agents - Google Patents
Angiopoietin-2 specific binding agents Download PDFInfo
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- WO2006045049A1 WO2006045049A1 PCT/US2005/037911 US2005037911W WO2006045049A1 WO 2006045049 A1 WO2006045049 A1 WO 2006045049A1 US 2005037911 W US2005037911 W US 2005037911W WO 2006045049 A1 WO2006045049 A1 WO 2006045049A1
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- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
Definitions
- the present invention relates to specific binding agents that recognize and bind to angiopoietin-2 (Ang-2). More specifically, the invention relates to the production, diagnostic use, and therapeutic use of monoclonal and polyclonal antibodies, and fragments thereof, which specifically bind Ang-2.
- Ang-2 angiopoietin-2
- Angiogenesis the formation of new blood vessels from existing ones, is essential to many physiological and pathological processes. Normally, angiogenesis is tightly regulated by pro- and anti-angiogenic factors, but in the case of diseases such as cancer, ocular neovascular diseases, arthritis, and psoriasis, the process can go awry. Folkman, J, Nat. Med., 1 :27-31 (1995). There are a number of diseases known to be associated with deregulated or undesired angiogenesis.
- Such diseases include, but are not limited to, ocular neovascularisation, such as retinopathies (including diabetic retinopathy), age- related macular degeneration, psoriasis, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, such as a rheumatoid or rheumatic inflammatory disease, especially arthritis (including rheumatoid arthritis), or other chronic inflammatory disorders, such as chronic asthma, arterial or post- transplantational atherosclerosis, endometriosis, and neoplastic diseases, for example so-called solid tumors and liquid (or hematopoietic) tumors (such as leukemias and lymphomas).
- retinopathies including diabetic retinopathy
- age- related macular degeneration psoriasis
- hemangioblastoma hemangioma
- arteriosclerosis arteriosclerosis
- inflammatory disease such as a rhe
- Tie-2 Tie-2 receptor tyrosine kinase
- Tie-2R Tie-2 receptor tyrosine kinase
- TalK murine Tie-2
- tek murine Tie-2
- angiopoietins Gale, N. W. and Yancopoulos, G. D., Genes Dev. 13:1055-1066 (1999)
- angiopoietins There are 4 known angiopoietins; angiopoietin-1 ("Ang-1") through angiopoietin-4 ("Ang-4"). These angiopoietins are also referred to as "Tie-2 ligands”.
- Ang-1 angiopoietin-1
- Ang-4 angiopoietin-4
- Tie-2 ligands ligands.
- the phenotypes of mouse Tie-2 and Ang-1 knockouts are similar and suggest that Ang-1 -stimulated Tie-2 phosphorylation mediates remodeling and stabilization of developing vessels in utero through maintenance of endothelial cell-support cell adhesion (Dumont, D. J., et al, Genes & Development, 5:1897- 1909 (1994); Sato, T. N., et al, Nature, 376:70-74 (1995); Suri, C, et al, (1996), supra).
- Ang-1 in vessel stabilization is thought to be conserved in the adult, where it is expressed widely and constitutively (Hanahan, D., Science, 277:48-50 (1997); Zagzag, D., et al, Experimental Neurology, 75P:391-400 (1999)).
- Ang-2 expression is primarily limited to sites of vascular remodeling, where it is thought to block Ang-1 function, thereby inducing a state of vascular plasticity conducive to angiogenesis (Hanahan, D., (1997), supra; Holash, J., et al, Science, 284:1994-1998 (1999); Maisonpierre, P. C, et al, (1997), supra).
- Ahmad et al (Cancer Res., 61 :1255-1259 (2001)) describe Ang-2 over-expression and show that it is purportedly associated with an increase in tumor growth in a mouse xenograft model. See also Etoh et al, supra, and Tanaka et al, supra, wherein data is presented purportedly associating Ang-2 over expression with tumor hypervascularity. However, in contrast, Yu et al. ⁇ Am. J. Path., 158:563-570 (2001)) report data to show that overexpression of Ang-2 in Lewis lung carcinoma and TA3 mammary carcinoma cells purportedly prolonged the survival of mice injected with the corresponding transfectants.
- An effective anti-Ang-2 therapy might benefit a vast population of cancer patients because most solid tumors require neovascularization to grow beyond 1 -2 millimeters in diameter. Such therapy might have wider application in other angiogenesis-associated diseases as well, such as retinopathies, arthritis, and psoriasis.
- the present invention provides an antibody comprising a heavy chain and a light chain, wherein said heavy chain comprises a heavy chain variable region selected from the group consisting of 526 HC (SEQ ID NO: 1); 528 HC (SEQ ID NO: 3); 531 HC (SEQ ID NO: 5); 533 HC (SEQ ID NO: 7); 535 HC (SEQ ID NO: 9); 536 HC (SEQ ID NO: 11); 537 HC (SEQ ID NO: 13); 540 HC (SEQ ID NO: 15); 543 HC (SEQ ID NO: 17); 544 HC (SEQ ID NO: 19); 545 HC (SEQ ID NO: 21); 546 HC (SEQ ID NO: 23); 551 HC (SEQ ID NO: 25); 553 HC (SEQ ID NO: 27); 555 HC (SEQ ID NO: 29); 558 HC (SEQ ID NO: 31); 559 HC (SEQ ID NO: 33); 565 HC (SEQ ID NO: 35); F
- the invention also provides a specific binding agent comprising at least one peptide selected from the group consisting of:
- the specific binding agent can be, for example, an antibody, such as a polyclonal, monoclonal, chimeric, humanized, or a fully human antibody.
- the antibody may also be a single chain antibody.
- the invention further relates to a hybridoma that produces a monoclonal antibody according to the invention.
- the invention relates to conjugates as described herein.
- the conjugate can be, for example, a specific binding agent (such as an antibody) of the invention.
- the invention further relates to nucleic acid molecules encoding the specific binding agents (such as an antibody) of the invention, as well as a vector comprising such nucleic acid molecule, as well as a host cell containing the vector. Additionally, the invention provides a method of making a specific binding agent comprising, (a) transforming a host cell with at least one nucleic acid molecule encoding the specific binding agent of Claim 1 ; (b) expressing the nucleic acid molecule in said host cell; and (c) isolating said specific binding agent.
- the invention further provides a method of making an antibody comprising: (a) transforming a host cell with at least one nucleic acid molecule encoding the antibody according to the invention; (b) expressing the nucleic acid molecule in said host cell; and (c) isolating said specific binding agent.
- the invention relates to a method of inhibiting undesired angiogenesis in a mammal by administering a therapeutically effective amount of a specific binding agent according to the invention.
- the invention also provides a method of treating cancer in a mammal by administering a therapeutically effective amount of a specific binding agent according to the invention.
- the invention also relates to a method of inhibiting undesired angiogenesis in a mammal comprising by administering a therapeutically effective amount of an antibody according to the invention.
- the invention additionally provides a method of treating cancer in a mammal comprising administering a therapeutically effective amount of antibody according to the invention.
- the invention further relates to pharmaceutical compositions comprising the specific binding agent according to the invention and a pharmaceutically acceptable formulation agent.
- the pharmaceutical composition may comprise an antibody according to the invention and a pharmaceutically acceptable formulation agent.
- the invention provides a method of modulating or inhibiting angiopoietin- 2 activity by administering one or more specific binding agents of the invention.
- the invention also provides a method of modulating or inhibiting angiopoietin-2 activity by administering an antibody of the invention.
- the invention further relates to a method of modulating at least one of vascular permeability or plasma leakage in a mammal comprising administering a therapeutically effective amount of the specific binding agent according to the invention.
- the invention also relates to a method of treating at least one of ocular neovascular disease, obesity, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, inflammatory disorders, atherosclerosis, endometriosis, neoplastic disease, bone-related disease, or psoriasis in a mammal comprising administering a therapeutically effective amount of a specific binding agent according to the invention.
- the invention further provides a method of modulating at least one of vascular permeability or plasma leakage in a mammal comprising administering a therapeutically effective amount of an antibody according to the invention.
- the invention also relates to a method of treating at least one of ocular neovascular disease, obesity, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, inflammatory disorders, atherosclerosis, endometriosis, neoplastic disease, bone-related disease, or psoriasis in a mammal comprising administering a therapeutically effective amount of an antibody according to the invention.
- the invention relates to a method of treating cancer in a mammal comprising administering a therapeutically effective amount of a specific binding agent according to the invention and a chemotherapeutic agent. It will be appreciated by those in the art that the specific binding agent and chemotherapeutic agent need not be administered simultaneously.
- the invention also relates to a method of treating cancer in a mammal comprising administering a therapeutically effective amount of an antibody according to the invention and a chemotherapeutic agent.
- the specific binding agent and chemotherapeutic agent need not be administered simultaneously.
- the invention also provides a specific binding agent comprising complementarity determining region 1 (CDR 1) of any of: 526 HC (SEQ ID NO: 1); 528 HC (SEQ ID NO: 3); 531 HC (SEQ ID NO: 5); 533 HC (SEQ ID NO: 7); 535 HC (SEQ ID NO: 9); 536 HC (SEQ ID NO: 11); 537 HC (SEQ ID NO: 13); 540 HC (SEQ ID NO: 15); 543 HC (SEQ ID NO: 17); 544 HC (SEQ ID NO: 19); 545 HC (SEQ ID NO: 21); 546 HC (SEQ ID NO: 23); 551 HC (SEQ ID NO: 25); 553 HC (S
- the invention further relates to a specific binding agent comprising complementarity determining region 2 (CDR 2) of any of: 526 HC (SEQ ID NO: 1); 528 HC (SEQ ID NO: 3); 531 HC (SEQ ID NO: 5); 533 HC (SEQ ID NO: 7); 535 HC (SEQ ID NO: 9); 536 HC (SEQ ID NO: 11); 537 HC (SEQ ID NO: 13); 540 HC (SEQ ID NO: 15); 543 HC (SEQ ID NO: 17); 544 HC (SEQ ID NO: 19); 545 HC (SEQ ID NO: 21); 546 HC (SEQ ID NO: 23); 551 HC (SEQ ID NO: 25); 553 HC (SEQ ID NO: 27); 555 HC (SEQ ID NO: 29); 558 HC (SEQ ID NO: 31); 559 HC (SEQ ID NO: 33); 565 HC (SEQ ID NO: 35); F1-C6 HC (SEQ
- the invention also relates to a specific binding agent comprising complementarity determining region 3 (CDR 3) of any of: 526 HC (SEQ ID NO: 1); 528 HC (SEQ ID NO: 3); 531 HC (SEQ ID NO: 5); 533 HC (SEQ ID NO: 7); 535 HC (SEQ ID NO: 9); 536 HC (SEQ ID NO: 11); 537 HC (SEQ ID NO: 13); 540 HC (SEQ ID NO: 15); 543 HC (SEQ ID NO: 17); 544 HC (SEQ ID NO: 19); 545 HC (SEQ ID NO: 21); 546 HC (SEQ ID NO: 23); 551 HC (SEQ ID NO: 25); 553 HC (SEQ ID NO: 27); 555 HC (SEQ ID NO: 29); 558 HC (SEQ ID NO: 31); 559 HC (SEQ ID NO: 33); 565 HC (SEQ ID NO: 35); F1-C6 HC (S
- the invention further provides a nucleic acid molecule encoding a specific binding agent according to the invention.
- the invention relates to a method of detecting the level of angiopoietin-2 in a biological sample by (a) contacting a specific binding agent of the invention with the sample; and (b) determining the extent of binding of the specific binding agent to the sample.
- the invention also relates to a method of detecting the level of angiopoietin-2 in a biological sample by (a) contacting an antibody of the invention with the sample; and (b) determining the extent of binding of the antibody to the sample.
- the invention also relates to a method of inhibiting undesired angiogenesis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein.
- the invention also relates to a method of modulating angiogenesis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein.
- the invention further relates to a method of inhibiting tumor growth characterized by undesired angiogenesis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein.
- the invention relates to a method of treating cancer in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein, and a chemotherapeutic agent.
- the chemotherapeutic agent is at least one of 5-FU, CPT- 11, and Taxotere. It will be appreciated, however, that other suitable chemotherapeutic agents and other cancer therapies can be used. It will be appreciated that the specific binding agents of the invention can be used to treat a number of diseases associated with deregulated or undesired angiogenesis.
- Such diseases include, but are not limited to, ocular neovascularisation, such as retinopathies (including diabetic retinopathy and age- related macular degeneration) psoriasis, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, such as a rheumatoid or rheumatic inflammatory disease, especially arthritis (including rheumatoid arthritis), or other chronic inflammatory disorders, such as chronic asthma, arterial or post- transplantational atherosclerosis, endometriosis, and neoplastic diseases, for example so-called solid tumors and liquid tumors (such as leukemias).
- retinopathies including diabetic retinopathy and age- related macular degeneration
- psoriasis hemangioblastoma
- hemangioma hemangioma
- arteriosclerosis inflammatory disease
- inflammatory disease such as a rheumatoid or r
- Additional diseases which can be treated by administration of the specific binding agents will be apparent to those skilled in the art.
- additional diseases include, but are not limited to, obesity, vascular permeability, plasma leakage, and bone-related disorders, including osteoporosis.
- the invention further relates to methods of treating these diseases associated with deregulated or undesired angiogenesis.
- Other embodiments of this invention will be readily apparent from the disclosure provided herewith.
- FIGURES Figure 1 depicts a graph of tumor size (y-axis) versus time (x-axis) in tumor bearing mice treated with either an anti-Ang-2 antibody (clone 533, 537 or 544) of the invention, with a control antibody, or with phosphate buffered saline (PBS). Details are described in the Examples.
- Figures 2A, 2B, and 2C depict epitope mapping data (O. D. 370) for full- length human Ang-2 (hAng-2), to the N-terminus of hAng-2, and to the C- terminus of hAng-2, respectively, for peptibodies TN8-Con4-C, L1-7-N, and 12- 9-3-C according to the invention, as well as for control peptibody, Tie2-Fc, C2B8, or 5Bl 2. Details are described in the Examples.
- Standard techniques may be used for recombinant DNA molecule, protein, and antibody production, as well as for tissue culture and cell transformation.
- Enzymatic reactions and purification techniques are typically performed according to the manufacturer's specifications or as commonly accomplished in the art using conventional procedures such as those set forth in Sambrook et al. (Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989)), or as described herein. Unless specific definitions are provided, the nomenclature utilized in connection with, and the laboratory procedures and techniques of analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques may be used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.
- Ang-2 refers to the polypeptide set forth in Figure 6 of U.S. Patent No. 6, 166, 185 ("Tie-2 ligand-2") or fragments thereof as well as related polypeptides which include allelic variants, splice variants, derivatives, substitution, deletions, and/or insertion variants, fusion peptides and polypeptides, and interspecies homologs.
- the Ang-2 polypeptide may or may not include additional terminal residues, e.g., leader sequences, targeting sequences, amino terminal methionine, amino terminal methionine and lysine residues, and/or tag or fusion proteins sequences, depending on the manner in which it is prepared.
- additional terminal residues e.g., leader sequences, targeting sequences, amino terminal methionine, amino terminal methionine and lysine residues, and/or tag or fusion proteins sequences, depending on the manner in which it is prepared.
- biologically active when used in relation to Ang-2 or an Ang-2 specific binding agent refers to a peptide or polypeptide having at least one activity characteristic of Ang-2 or of an Ang-2 specific binding agent.
- a specific binding agent of Ang-2 may have agonist, antagonist, or neutralizing or blocking activity with respect to at least one biological activity of Ang-2.
- specific binding agent refers to a molecule, preferably a proteinaceous molecule, that binds Ang-2 (and variants and derivatives thereof as defined herein) with a greater affinity than other angiopoietins.
- a specific binding agent may be a protein, peptide, nucleic acid, carbohydrate, lipid, or small molecular weight compound which binds preferentially to Ang-2.
- the specific binding agent according to the present invention is an antibody, such as a polyclonal antibody, a monoclonal antibody (mAb), a chimeric antibody, a CDR-grafted antibody, a multi-specific antibody, a bi-specific antibody, a catalytic antibody, a humanized antibody, a human antibody, an anti- idiotypic (anti-Id) antibody, and antibodies that can be labeled in soluble or bound form, as well as fragments, variants or derivatives thereof, either alone or in combination with other amino acid sequences, provided by known techniques. Such techniques include, but are not limited to enzymatic cleavage, chemical cleavage, peptide synthesis or recombinant techniques.
- the anti-Ang-2 specific binding agents of the present invention are capable of binding portions of Ang-2 that modulate, e.g., inhibit or promote, the biological activity of Ang-2 and/or other Ang-2-associated activities.
- polyclonal antibody refers to a heterogeneous mixture of antibodies that recognize and bind to different epitopes on the same antigen.
- Polyclonal antibodies may be obtained from crude serum preparations or may be purified using, for example, antigen affinity chromatography, or Protein A/Protein G affinity chromatography.
- monoclonal antibodies refers to a collection of antibodies encoded by the same nucleic acid molecule which are optionally produced by a single hybridoma or other cell line, or by a transgenic mammal such that each monoclonal antibody will typically recognize the same epitope on the antigen.
- the term “monoclonal” is not limited to any particular method for making the antibody, nor is the term limited to antibodies produced in a particular species, e.g., mouse, rat, etc.
- chimeric antibodies refers to antibodies in which a portion of the heavy and/or light chain is identical with or homologous to a corresponding sequence in an antibody derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is/are identical with or homologous to a corresponding sequence in antibodies derived from another species or belonging to another antibody class or subclass. Also included are fragments of such antibodies, that exhibit the desired biological activity (i.e., the ability to specifically bind Ang-2). See, U.S. Patent No. 4,816,567 and Morrison et al, Proc Natl Acad Sci (USA), 81 :6851-6855 (1985).
- CDR grafted antibody refers to an antibody in which the CDR from one antibody of a particular species or isotype is recombinantly inserted into the framework of another antibody of the same or different species or isotype.
- multi-specific antibody refers to an antibody having variable regions that recognize more than one epitope on one or more antigens.
- a subclass of this type of antibody is a "bi-specific antibody” which recognizes two distinct epitopes on the same or different antigens.
- Catalytic antibodies refers to antibodies wherein one or more cytotoxic, or more generally one or more biologically active, moieties are attached to the targeting binding agent.
- humanized antibody refers to a specific type of CDR-grafted antibody in which the antibody framework region is derived from a human but each CDR is replaced with that derived from another species, such as a murine CDR.
- CDR is defined infra.
- fully human antibody refers to an antibody in which both the
- CDR and the framework are derived from one or more human DNA molecules.
- anti-idiotype antibody refers to any antibody that specifically binds to another antibody that recognizes an antigen. Production of anti-idiotype antibodies can be performed by any of the methods described herein for production of an Ang-2-specific antibodies except that these antibodies arise from e.g., immunization of an animal with an Ang-2-specific antibody or Ang-2- binding fragment thereof, rather than Ang-2 polypeptide itself or a fragment thereof.
- variants include those polypeptides wherein amino acid residues are inserted into, deleted from and/or substituted into the naturally occurring (or at least a known) amino acid sequence for the binding agent.
- variants of the invention include fusion proteins as described below.
- Derivatives include those binding agents that have been chemically modified in some manner distinct from insertion, deletion, or substitution variants.
- “Specifically binds Ang-2” refers to the ability of a specific binding agent
- Ang-2 polypeptide such as an antibody or fragment thereof
- affinity as determined by, e.g., Affinity ELISA or BIAcore assays as described herein
- neutralization capability as determined by e.g., Neutralization ELISA assays described herein, or similar assays
- affinity is determined by, e.g., Affinity ELISA or BIAcore assays as described herein
- neutralization capability as determined by e.g., Neutralization ELISA assays described herein, or similar assays
- antigen binding domain or "antigen binding region” refers to that portion of the specific binding agent (such as an antibody molecule) which contains the specific binding agent amino acid residues (or other moieties) that interact with an antigen and confer on the binding agent its specificity and affinity for the antigen.
- the antigen-binding domain is commonly referred to as the "complementarity-determining region, or CDR.”
- epitope refers to that portion of any molecule capable of being recognized by and bound by a specific binding agent, e.g. an antibody, at one or more of the binding agent's antigen binding regions.
- Epitopes usually consist of chemically active surface groupings of molecules, such as for example, amino acids or carbohydrate side chains, and have specific three-dimensional structural characteristics as well as specific charge characteristics.
- Epitopes as used herein may be contiguous or non-contiguous.
- epitopes may be mimetic in that they comprise a three dimensional structure that is identical to the epitope used to generate the antibody, yet comprise none or only some of the amino acid residues found in the Ang-2 used to stimulate the antibody immune response.
- inhibiting and/or neutralizing epitope is an epitope, which when bound by a specific binding agent such as an antibody, results in the loss of (or at least the decrease in) biological activity of the molecule, cell, or organism containing such epitope, in vivo, in vitro, or in situ.
- the neutralizing epitope is located on or is associated with a biologically active region of Ang-2.
- activating epitope is an epitope, which when bound by a specific binding agent of the invention, such as an antibody, results in activation, or at least maintenance of a biologically active conformation, of Ang-2.
- antibody fragment refers to a peptide or polypeptide which comprises less than a complete, intact antibody.
- Complete antibodies comprise two functionally independent parts or fragments: an antigen binding fragment known as "Fab," and a carboxy terminal crystallizable fragment known as the "Fc" fragment.
- the Fab fragment includes the first constant domain from both the heavy and light chain (CHl and CLl) together with the variable regions from both the heavy and light chains that bind the specific antigen.
- Each of the heavy and light chain variable regions includes three complementarity determining regions (CDRs) and framework amino acid residues which separate the individual CDRs.
- the Fc region comprises the second and third heavy chain constant regions (CH2 and CH3) and is involved in effector functions such as complement activation and attack by phagocytic cells.
- the Fc and Fab regions are separated by an antibody "hinge region," and depending on how the full length antibody is proteolytically cleaved, the hinge region may be associated with either the Fab or Fc fragment.
- the hinge region may be associated with either the Fab or Fc fragment.
- cleavage of an antibody with the protease papain results in the hinge region being associated with the resulting Fc fragment, while cleavage with the protease pepsin provides a fragment wherein the hinge is associated with both Fab fragment simultaneously. Because the two Fab fragments are in fact covalently linked following pepsin cleavage, the resulting fragment is termed the F(ab')2 fragment.
- An Fc domain may have a relatively long serum half-life, whereas a Fab is short-lived. (Capon et al, Nature, 337: 525-31 (1989)) When expressed as part of a fusion protein, an Fc domain can impart longer half-life or incorporate such functions as Fc receptor binding, Protein A binding, complement fixation and perhaps even placental transfer into the protein to which it is fused.
- the Fc region may be a naturally occurring Fc region, or may be altered to improve certain qualities, such as therapeutic qualities or circulation time.
- variable region refers to a portion of the light and/or heavy chains of an antibody, typically including approximately the amino-terminal 120 to 130 amino acids in the heavy chain and about 100 to 110 amino terminal amino acids in the light chain.
- the variable regions typically differ extensively in amino acid sequence even among antibodies of the same species.
- the variable region of an antibody typically determines the binding and specificity of each particular antibody for its particular antigen. The variability in sequence is concentrated in those regions referred to as complementarity- determining regions (CDRs), while the more highly conserved regions in the variable domain are called framework regions (FR).
- CDRs complementarity- determining regions
- FR framework regions
- the CDRs of the light and heavy chains contain within them the amino acids which are largely responsible for the direct interaction of the antibody with antigen, however, amino acids in the FRs can significantly affect antigen binding/recognition as discussed herein infra.
- the term "light chain” when used in reference to an antibody collectively refers to two distinct types, called kappa (k) or lambda (1) based on the amino acid sequence of the constant domains.
- the combination of heavy and light chains give rise to five known classes of antibodies: IgA, IgD, IgE, IgG and IgM, respectively, including four known subclasses of IgG, designated as IgGi, IgG 2 , IgG 3 and IgG 4 .
- Naturally occurring when used in connection with biological materials such as nucleic acid molecules, polypeptides, host cells, and the like, refers to those which are found in nature and not modified by a human being.
- isolated when used in relation to Ang-2 or to a specific binding agent of Ang-2 refers to a compound that is free from at least one contaminating polypeptide or compound that is found in its natural environment, and preferably substantially free from any other contaminating mammalian polypeptides that would interfere with its therapeutic or diagnostic use.
- mature when used in relation to Ang-2, anti-Ang-2 antibody, or to any other proteinaceous specific binding agent of Ang-2 refers to a peptide or a polypeptide lacking a leader or signal sequence.
- the "mature" peptide or polypeptide may also include additional amino acid residues (but still lack a leader sequence) such as an amino terminal methionine, or one or more methionine and lysine residues.
- additional amino acid residues but still lack a leader sequence
- a peptide or polypeptide produced in this manner may be utilized with or without these additional amino acid residues having been removed.
- Ang-2 refers to an amount of a specific binding agent that is useful or necessary to support an observable change in the level of one or more biological activities of Ang-2.
- the change may be either an increase or decrease in the level of Ang-2 activity.
- the change is a decrease in Ang-2 activity.
- Suitable specific binding agents include, but are not limited to, antibodies and derivatives thereof, polypeptides, and small molecules. Suitable specific binding agents may be prepared using methods known in the art.
- An exemplary Ang-2 polypeptide specific binding agent of the present invention is capable of binding a certain portion of the Ang-2 polypeptide, and preferably modulating the activity or function of Ang-2 polypeptide.
- antibodies and antibody fragments that specifically bind Ang-2 polypeptides are within the scope of the present invention.
- the antibodies may be polyclonal including mono-specific polyclonal, monoclonal (mAbs), recombinant, chimeric, humanized such as CDR-grafted, human, single chain, catalytic, multi-specific and/or bi-specific, as well as fragments, variants, and/or derivatives thereof.
- Polyclonal antibodies directed toward an Ang-2 polypeptide generally are produced in animals ⁇ e.g. , rabbits, hamsters, goats, sheep, horses, pigs, rats, gerbils, guinea pigs, mice, or any other suitable mammal, as well as other non- mammal species) by means of multiple subcutaneous or intraperitoneal injections of Ang-2 polypeptide or a fragment thereof with or without an adjuvant.
- Such adjuvants include, but are not limited to, Freund's complete and incomplete, mineral gels such as aluminum hydroxide, and surface-active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, and dinitrophenol.
- BCG Bacilli Calmette-Guerin
- Corynebacterium parvum are potentially useful human adjuvants. It may be useful to conjugate an antigen polypeptide to a carrier protein that is immunogenic in the species to be immunized, such as keyhole limpet hemocyanin, serum, albumin, bovine thyroglobulin, or soybean trypsin inhibitor.
- aggregating agents such as alum are used to enhance the immune response.
- the animals are bled and the serum is assayed for anti-Ang-2 polypeptide antibody titer which can be determined using the assays described herein under "Examples".
- Polyclonal antibodies may be utilized in the sera from which they were detected, or may be purified from the sera, using, for example, antigen affinity chromatography or Protein A or G affinity chromatography.
- Monoclonal antibodies directed toward Ang-2 polypeptides can be produced using, for example but without limitation, the traditional "hybridoma” method or the newer “phage display” technique.
- monoclonal antibodies of the invention may be made by the hybridoma method as described in Kohler et al, Nature 256:495 (1975); the human B-cell hybridoma technique (Kosbor et al, Immunol Today 4:72 (1983); Cote et al, Proc Natl Acad Sci (USA) 80: 2026-2030 (1983); Brön et al, Monoclonal Antibody Production Techniques and Applications, pp.
- myeloma cell lines can be used.
- Such cell lines suited for use in hybridoma-producing fusion procedures preferably are non-antibody-producing, have high fusion efficiency, and enzyme deficiencies that render them incapable of growing in certain selective media which support the growth of only the desired fused cells (hybridomas).
- cell lines used in mouse fusions are Sp-20, P3-X63/Ag8, P3-X63- Ag8.653, NSl/l.Ag 4 1, Sp210-Agl4, FO, NSO/U, MPC-11, MPCl 1-X45 -GTG 1.7 and S194/5XX0 BuI; cell lines used in rat fusions are R210.RCY3, Y3-Ag 1.2.3, IR983F and 4B210.
- Other cell lines useful for cell fusions are U-266, GM1500-GRG2, LICR-LON-HMy2 and UC729-6. Hybridomas and other cell lines that produce monoclonal antibodies are contemplated to be novel compositions of the present invention.
- the phage display technique may also be used to generate monoclonal antibodies from any species. Preferably, this technique is used to produce fully human monoclonal antibodies in which a polynucleotide encoding a single Fab or Fv antibody fragment is expressed on the surface of a phage particle.
- a polynucleotide encoding a single Fab or Fv antibody fragment is expressed on the surface of a phage particle.
- host cells either eukaryotic or prokaryotic, may be used to express the monoclonal antibody polynucleotides using recombinant techniques well known and routinely practiced in the art.
- transgenic animals are produced wherein a polynucleotide encoding the desired specific binding agent is introduced into the genome of a recipient animal, such as, for example, a mouse, rabbit, goat, or cow, in a manner that permits expression of the polynucleotide molecules encoding a monoclonal antibody or other specific binding agent.
- the polynucleotides encoding the monoclonal antibody or other specific binding agent can be ligated to mammary-specific regulatory sequences, and the chimeric polynucleotides can be introduced into the germline of the target animal.
- the resulting transgenic animal then produces the desired antibody in its milk (Pollock et al, J Immunol Meth 231 : 147-157 (1999); Little et al, Immunol Today 8:364-370 (2000)).
- plants may be used to express and produce Ang-2 specific binding agents such as monoclonal antibodies by transfecting suitable plants with the polynucleotides encoding the monoclonal antibodies or other specific binding agents.
- a monoclonal or polyclonal antibody or fragment thereof that is derived from other than a human species may be "humanized” or “chimerized”.
- Methods for humanizing non- human antibodies are well known in the art. (see U.S. Patent Nos. 5,859,205, 5,585,089, and 5,693,762). Humanization is performed, for example, using methods described in the art (Jones et al, Nature 321 : 522-525 (1986); Riechmann et al, Nature, 332: 323-327 (1988); Verhoeyen et al, Science 239:1534-1536 (1988)) by substituting at least a portion of, e.g.
- CDRs complementarity-determining region
- Ang-2 polypeptides as well as, fragments, variants and/or derivatives thereof.
- Such antibodies can be produced using the phage display technique described above.
- transgenic animals e.g., mice
- Ang-2 antigen or fragments thereof where the Ang-2 fragments have an amino acid sequence that is unique to Ang-2.
- Such immunogens can be optionally conjugated to a carrier.
- transgenic animals are produced by incapacitating the endogenous loci encoding the heavy and light immunoglobulin chains therein, and inserting loci encoding human heavy and light chain proteins into the genome thereof.
- Partially modified animals that are those having less than the full complement of these modifications, are then crossbred to obtain an animal having all of the desired immune system modifications.
- these transgenic animals When administered an immunogen, these transgenic animals are capable of producing antibodies with human variable regions, including human (rather than e.g., murine) amino acid sequences, that are immuno-specific for the desired antigens. See PCT application Nos., PCT/US96/05928 and PCT/US93/06926. Additional methods are described in U.S. Patent No. 5,545,807, PCT application Nos. PCT/US91/245,
- Human antibodies may also be produced by the expression of recombinant DNA in host cells or by expression in hybridoma cells as described herein.
- Transgenesis is achieved in a number of different ways. See, for example, Bruggeman et al, Immunol Today 77:391-7 (1996).
- a minilocus is constructed such that gene segments in a germline configuration are brought artificially close to each other. Due to size limitations (i.e., having generally less than 30 kb), the resulting minilocus will contain a limited number of differing gene segments, but is still capable of producing a large repertoire of antibodies.
- Miniloci containing only human DNA sequences, including promoters and enhancers are fully functional in the transgenic mouse.
- yeast artificial chromosomes When larger number of gene segments are desired in the transgenic animal, yeast artificial chromosomes (YACs) are utilized. YACs can range from several hundred kilobases to 1 Mb and are introduced into the mouse (or other appropriate animal) genome via microinjection directly into an egg or via transfer of the YAC into embryonic stem (ES)-cell lines. In general, YACs are transferred into ES cells by lipofection of the purified DNA, or yeast spheroplast fusion wherein the purified DNA is carried in micelles and fusion is carried out in manner similar to hybridoma fusion protocols. Selection of desired ES cells following DNA transfer is accomplished by including on the YAC any of the selectable markers known in the art.
- bacteriophage Pl vectors are used which are amplified in a bacterial E. coli host. While these vectors generally carry less inserted DNA than a YAC, the clones are readily grown in high enough yield to permit direct microinjection into a mouse egg. Use of a cocktail of different Pl vectors has been shown to lead to high levels of homologous recombination.
- transgenic mouse or other appropriate animal
- any of the techniques known in the art to detect serum levels of a circulating antibody e.g., ELISA
- the transgenic animal is crossed with a mouse in which the endogenous Ig locus has been disrupted.
- the result provides progeny wherein essentially all B cells express human antibodies.
- the entire animal Ig locus is replaced with the human Ig locus, wherein the resulting animal expresses only human antibodies.
- portions of the animal's locus are replaced with specific and corresponding regions in the human locus.
- the animals resulting from this procedure may express chimeric antibodies, as opposed to fully human antibodies, depending on the nature of the replacement in the mouse Ig locus.
- Human antibodies can also be produced by exposing human splenocytes (B or T cells) to an antigen in vitro, then reconstituting the exposed cells in an immunocompromised mouse, e.g. SCID or nod/SCID. See Brams et ah, J Immunol, 160: 2051-2058 (1998); Carballido et ah, Nat Med, 6: 103-106 (2000).
- SCID-hu engraftment of human fetal tissue into SCID mice
- SCID-hu results in long-term hematopoiesis and human T-cell development
- Any humoral immune response in these chimeric mice is completely dependent on co-development of T-cells in the animals (Martensson et ah, Immunol 53:1271- 179 (1994)).
- human peripheral blood lymphocytes are transplanted intraperitoneal Iy (or otherwise) into SCID mice (Mosier et al.
- SEA Staphylococcal Enterotoxin A
- SEA Staphylococcal Enterotoxin A
- anti-human CD40 monoclonal antibodies SEA
- an entirely synthetic human heavy chain repertoire is created from unrearranged V gene segments by assembling each human VH segment with D segments of random nucleotides together with a human J segment (Hoogenboom et al, JMoI Biol 227:381-388 (1992)).
- a light chain repertoire is constructed by combining each human V segment with a J segment (Griffiths et al, EMBO J 75:3245-3260 (1994)).
- Nucleotides encoding the complete antibody i.e., both heavy and light chains
- this polynucleotide is ligated to a nucleotide encoding a filamentous phage minor coat protein. When this fusion protein is expressed on the surface of the phage, a polynucleotide encoding a specific antibody is identified by selection using an immobilized antigen.
- antibody fragments are assembled as two Fab fragments by fusion of one chain to a phage protein and secretion of the other into bacterial periplasm (Hoogenboom et al, Nucl Acids Res 19:4133-4137 (1991); Barbas et al, Proc Natl Acad Sci (USA) 88:7978-7982 (1991)).
- chimeric, humanized, CDR-grafted, and fully human antibodies, or fragments thereof are typically produced by recombinant methods.
- Polynucleotide molecule(s) encoding the heavy and light chains of each antibody or fragments thereof can be introduced into host cells and expressed using materials and procedures described herein.
- the antibodies are produced in mammalian host cells, such as CHO cells. Details of such production is described below.
- the polypeptides comprising the amino acid sequence variable domains of Ang-2 antibodies may be fused at either the N-terminus or the C-terminus to one or more domains of an Fc region of human IgG.
- an Fc domain can provide longer half-life or incorporate such functions as Fc receptor binding, Protein A binding, complement fixation and perhaps even placental transfer. (Capon et ⁇ /., Nature, 337: 525-531 (1989)).
- the antibody hinge, CH2 and CH3 regions may be fused at either the N-terminus or C-terminus of the specific binding agent polypeptides such as an anti-Ang-2 Fab or Fv fragment (obtained, e.g., from a phage display library) using methods known to the skilled artisan.
- the resulting fusion protein may be purified by use of a Protein A or Protein G affinity column. Peptides and proteins fused to an Fc region have been found to exhibit a substantially greater half-life in vivo than the unfused counterpart. Also, a fusion to an Fc region allows for dimerization/multimerization of the fusion polypeptide.
- the Fc region may be a naturally occurring Fc region, or may be altered to improve certain qualities, such as therapeutic qualities, circulation time, decrease aggregation problems, etc.
- Other examples known in the art include those wherein the Fc region, which may be human or another species, or may be synthetic, is fused to the N-terminus of CD30L to treat Hodgkin's Disease, anaplastic lymphoma and T- cell leukemia (U.S. Patent No. 5,480,981), the Fc region is fused to the TNF receptor to treat septic shock (Fisher et al, N Engl J Med, 334: 1697-1702
- Catalytic antibodies are another type of fusion molecule and include antibodies to which one or more cytotoxic, or more generally one or more biologically active, moieties are attached to the specific binding agent. See, for example (Rader et al. , Chem Eur J 12:2091-2095 (2000)). Cytotoxic agents of this type improve antibody-mediated cytotoxicity, and include such moieties as cytokines that directly or indirectly stimulate cell death, radioisotopes, chemotherapeutic drugs (including prodrugs), bacterial toxins (ex. pseudomonas exotoxin, diphtheria toxin, etc.), plant toxins (ex.
- the cytotoxic agent can be "attached" to one component of a bi-specific or multi-specific antibody by binding of this agent to one of the alternative antigen recognition sites on the antibody.
- protein cytotoxins can be expressed as fusion proteins with the specific binding agent following ligation of a polynucleotide encoding the toxin to a polynucleotide encoding the binding agent.
- the specific binding agent can be covalently modified to include the desired cytotoxin.
- fusion proteins are immunogenic polypeptides, proteins with long circulating half lives, such as immunoglobulin constant regions, marker proteins, proteins or polypeptides that facilitate purification of the desired specific binding agent polypeptide, and polypeptide sequences that promote formation of multimeric proteins (such as leucine zipper motifs that are useful in dimer formation/stability).
- This type of insertional variant generally has all or a substantial portion of the native molecule, linked at the N- or C-terminus, to all or a portion of a second polypeptide.
- fusion proteins typically employ leader sequences from other species to permit the recombinant expression of a protein in a heterologous host.
- Another useful fusion protein includes the addition of an immunologically active domain, such as an antibody epitope, to facilitate purification of the fusion protein. Inclusion of a cleavage site at or near the fusion junction will facilitate removal of the extraneous polypeptide after purification.
- Other useful fusions include linking of functional domains, such as active sites from enzymes, glycosylation domains, cellular targeting signals or transmembrane regions. There are various commercially available fusion protein expression systems that may be used in the present invention.
- GST glutathione-S-transferase
- NEB maltose binding protein
- FLAG IBI, New Haven, CT
- 6xHis 6xHis system
- N-terminal fusion that is contemplated to be useful is the fusion of a Met-Lys dipeptide at the N-terminal region of the protein or peptides. Such a fusion may produce beneficial increases in protein expression or activity.
- a particularly useful fusion construct may be one in which a specific binding agent peptide is fused to a hapten to enhance immunogenicity of a specific binding agent fusion construct which is useful, for example, in the production of anti-idiotype antibodies of the invention.
- fusion constructs to increase immunogenicity are well known to those of skill in the art, for example, a fusion of specific binding agent with a helper antigen such as hsp70 or peptide sequences such as from diphtheria toxin chain or a cytokine such as IL-2 will be useful in eliciting an immune response.
- fusion construct can be made which will enhance the targeting of the antigen binding agent compositions to a specific site or cell.
- fusion constructs including heterologous polypeptides with desired properties, e.g., an Ig constant region to prolong serum half-life or an antibody or fragment thereof for targeting also are contemplated.
- Other fusion systems produce polypeptide hybrids where it is desirable to excise the fusion partner from the desired polypeptide.
- the fusion partner is linked to the recombinant specific binding agent polypeptide by a peptide sequence containing a specific recognition sequence for a protease. Examples of suitable sequences are those recognized by the Tobacco Etch Virus protease (Life Technologies, Gaithersburg, MD) or Factor Xa (New England Biolabs, Beverley, MA.
- the invention also provides fusion polypeptides comprising all or part of a variable domain of an Ang-2 antibody, such as a heavy chain variable region with an amino acid sequence as described herein or a light chain variable region with an amino acid sequence as described herein in combination with truncated tissue factor (tTF), a vascular targeting agent consisting of a truncated form of a human coagulation-inducing protein that acts as a tumor blood vessel clotting agent.
- tTF tissue factor
- the fusion of tTF to the anti- Ang-2 antibody, or fragments thereof may facilitate the delivery of anti -Ang-2 to target cells.
- Variants of Specific Binding Agents of the present invention include insertion, deletion, and/or substitution variants.
- insertion variants are provided wherein one or more amino acid residues supplement a specific binding agent amino acid sequence. Insertions may be located at either or both termini of the protein, or may be positioned within internal regions of the specific binding agent amino acid sequence. Insertional variants with additional residues at either or both termini can include, for example, fusion proteins and proteins including amino acid tags or labels. Insertion variants include specific binding agent polypeptides wherein one or more amino acid residues are added to a specific binding agent amino acid sequence, or fragment thereof.
- Variant products of the invention also include mature specific binding agent products.
- Such specific binding agent products have the leader or signal sequences removed, however the resulting protein has additional amino terminal residues as compared to wild-type Ang-2 polypeptide.
- the additional amino terminal residues may be derived from another protein, or may include one or more residues that are not identifiable as being derived from a specific protein.
- Specific binding agent products with an additional methionine residue at position - 1 are contemplated, as are specific binding agent products with additional methionine and lysine residues at positions -2 and -1 (Met ⁇ -Lys "1 -specific binding agent).
- Variants of specific binding agents having additional Met, Met-Lys, Lys residues (or one or more basic residues in general) are particularly useful for enhanced recombinant protein production in bacterial host cells.
- the invention also embraces specific binding agent variants having additional amino acid residues that arise from use of specific expression systems.
- use of commercially available vectors that express a desired polypeptide as part of glutathione-S-transferase (GST) fusion product provides the desired polypeptide having an additional glycine residue at amino acid position -1 after cleavage of the GST component from the desired polypeptide.
- GST glutathione-S-transferase
- Variants which result from expression in other vector systems are also contemplated, including those wherein poly-histidine tags are incorporated into the amino acid sequence, generally at the carboxy and/or amino terminus of the sequence.
- Insertional variants also include fusion proteins as described above, wherein the amino and/or carboxy termini of the specific binding agent- polypeptide is fused to another polypeptide, a fragment thereof, or amino acid sequences which are not generally recognized to be part of any specific protein sequence.
- the invention provides deletion variants wherein one or more amino acid residues in a specific binding agent polypeptide are removed. Deletions can be effected at one or both termini of the specific binding agent polypeptide, or from removal of one or more residues within the specific binding agent amino acid sequence. Deletion variants necessarily include all fragments of a specific binding agent polypeptide.
- Antibody fragments include those portions of the antibody that bind to an epitope on the antigen polypeptide. Examples of such fragments include Fab and F(ab') 2 fragments generated, for example, by enzymatic or chemical cleavage of full-length antibodies. Other binding fragments include those generated by recombinant DNA techniques, such as the expression of recombinant plasmids containing nucleic acid sequences encoding antibody variable regions.
- the invention also embraces polypeptide fragments of an Ang-2 binding agent wherein the fragments maintain the ability to specifically bind an Ang-2 polypeptide.
- Fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 or more consecutive amino acids of a peptide or polypeptide of the invention are comprehended herein.
- Preferred polypeptide fragments display immunological properties unique to or specific for the antigen-binding agent so of the invention. Fragments of the invention having the desired immunological properties can be prepared by any of the methods well known and routinely practiced in the art.
- the invention provides substitution variants of specific binding agents of the invention. Substitution variants are generally considered to be "similar" to the original polypeptide or to have a certain "percent identity" to the original polypeptide, and include those polypeptides wherein one or more amino acid residues of a polypeptide are removed and replaced with alternative residues. In one aspect, the substitutions are conservative in nature, however, the invention embraces substitutions that are also non-conservative.
- Preferred methods to determine the relatedness or percent identity of two polypeptides are designed to give the largest match between the sequences tested. Methods to determine identity are described in publicly available computer programs. Preferred computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package, including GAP (Devereux et al, Nucl. Acid. Res., 12:387 (1984); Genetics Computer Group, University of Wisconsin, Madison, WI, BLASTP, BLASTN, and FASTA (Altschul et al., J. MoI. Biol, 215:403-410 (1990)). The BLASTX program is publicly available from the National Center for Biotechnology Information (NCBI) and other sources ⁇ BLAST Manual, Altschul et al. NCB/NLM/NIH Bethesda, MD 20894; Altschul et al, supra (1990)). The well- known Smith Waterman algorithm may also be used to determine identity.
- NCBI National Center for Biotechnology Information
- the selected alignment method will result in an alignment that spans at least ten percent of the full length of the target polypeptide being compared, i.e., at least 40 contiguous amino acids where sequences of at least 400 amino acids are being compared, 30 contiguous amino acids where sequences of at least 300 to about 400 amino acids are being compared, at least 20 contiguous amino acids where sequences of 200 to about 300 amino acids are being compared, and at least 10 contiguous amino acids where sequences of about 100 to 200 amino acids are being compared.
- GAP Genetics Computer Group, University of Wisconsin, Madison, WI
- two polypeptides for which the percent sequence identity is to be determined are aligned for optimal matching of their respective amino acids (the "matched span", as determined by the algorithm).
- a gap opening penalty which is typically calculated as 3X the average diagonal; the "average diagonal” is the average of the diagonal of the comparison matrix being used; the “diagonal” is the score or number assigned to each perfect amino acid match by the particular comparison matrix
- a gap extension penalty which is usually 1/10 times the gap opening penalty
- a comparison matrix such as PAM 250 or BLOSUM 62
- a standard comparison matrix (see Dayhoff et al , Atlas of Protein Sequence and Structure, 5(3)(1978) for the PAM 250 comparison matrix; Henikoff et al, Proc. Natl. Acad. Sci USA, 89:10915-10919 (1992) for the BLOSUM 62 comparison matrix) is also used by the algorithm.
- the parameters for a polypeptide sequence comparison include the following:
- the GAP program may be useful with the above parameters.
- the aforementioned parameters are the default parameters for polypeptide comparisons (along with no penalty for end gaps) using the GAP algorithm.
- the parameters for polynucleotide molecule sequence comparisons include the following:
- the GAP program may also be useful with the above parameters.
- the aforementioned parameters are the default parameters for polynucleotide molecule comparisons.
- gap opening penalties may be used, including those set forth in the Program Manual, Wisconsin Package, Version 9, September, 1997.
- the particular choices to be made will be apparent to those of skill in the art and will depend on the specific comparison to be made, such as DNA-to-DNA, protein-to-protein, protein-to-DNA; and additionally, whether the comparison is between given pairs of sequences (in which case GAP or BestFit are generally preferred) or between one sequence and a large database of sequences (in which case FASTA or BLASTA are preferred).
- the amino acids may have either L or D stereochemistry (except for GIy, which is neither L nor D) and the polypeptides and compositions of the present invention may comprise a combination of stereochemistries. However, the L stereochemistry is preferred.
- the invention also provides reverse molecules wherein the amino terminal to carboxy terminal sequence of the amino acids is reversed. For example, the reverse of a molecule having the normal sequence X r X 2 -X 3 would be X 3 -X 2 -Xi.
- the invention also provides retro-reverse molecules wherein, as above, the amino terminal to carboxy terminal sequence of amino acids is reversed and residues that are normally "L" enantiomers are altered to the "D" stereoisomer form.
- Stereoisomers e.g., D-amino acids of the twenty conventional amino acids, unnatural amino acids such as ⁇ -, ⁇ -disubstituted amino acids, N-alkyl amino acids, lactic acid, and other unconventional amino acids may also be suitable components for polypeptides of the present invention.
- unconventional amino acids include, without limitation: aminoadipic acid, beta- alanine, beta-aminopropionic acid, aminobutyric acid, piperidinic acid, aminocaprioic acid, aminoheptanoic acid, aminoisobutyric acid, aminopimelic acid, diaminobutyric acid, desmosine, diaminopimelic acid, diaminopropionic acid, N-ethylglycine, N-ethylaspargine, hyroxylysine, allo-hydroxylysine, hydroxyproline, isodesmosine, allo-isoleucine, N-methylglycine, sarcosine, N- methylisoleucine, N-methylvaline, norvaline, norleucine, orithine, 4- hydroxyproline, ⁇ -carboxyglutamate, ⁇ -N,N,N-trimethyllysine, ⁇ -N-acetyllysine, O-phosphoserine, N-
- the left-hand end of single-stranded polynucleotide sequences is the 5' end; the left-hand direction of double-stranded polynucleotide sequences is referred to as the 5' direction.
- the direction of 5' to 3'- addition of nascent RNA transcripts is referred to as the transcription direction; sequence regions on the DNA strand having the same sequence as the RNA and which are 5 ? to the 5' end of the RNA transcript are referred to as "upstream sequences"; sequence regions on the DNA strand having the same sequence as the RNA and which are 3' to the 3' end of the RNA transcript are referred to as "downstream sequences".
- Conservative amino acid substitutions may encompass non-naturally occurring amino acid residues, which are typically incorporated by chemical peptide synthesis rather than by synthesis in biological systems. These include peptidomimetics and other reversed or inverted forms of amino acid moieties.
- Naturally occurring residues may be divided into classes based on common side chain properties:
- Trp Trp
- Tyr Phe
- non-conservative substitutions may involve the exchange of a member of one of these classes for a member from another class.
- substituted residues may be introduced into regions of the human antibody that are homologous with non-human antibodies, or into the non-homologous regions of the molecule.
- the hydropathic index of amino acids may be considered.
- Each amino acid has been assigned a hydropathic index on the basis of its hydrophobicity and charge characteristics. They are: isoleucine (+4.5); valine (+4.2); leucine (+3.8); phenylalanine (+2.8); cysteine/cystine (+2.5); methionine (+1.9); alanine (+1.8); glycine (-0.4); threonine (-0.7); serine (-0.8); tryptophan (-0.9); tyrosine (-1.3); proline (-1.6); histidine (-3.2); glutamate (-3.5); glutamine (-3.5); aspartate (-3.5); asparagine (-3.5); lysine (-3.9); and arginine (-4.5).
- hydropathic amino acid index in conferring interactive biological function on a protein is understood in the art. Kyte et al. , J. MoI. Biol, 157:105-131 (1982). It is known that certain amino acids may be substituted for other amino acids having a similar hydropathic index or score and still retain a similar biological activity. In making changes based upon the hydropathic index, in certain embodiments, the substitution of amino acids whose hydropathic indices are within ⁇ 2 is included. In certain embodiments, those which are within ⁇ 1 are included, and in certain embodiments, those within ⁇ 0.5 are included.
- the substitution of like amino acids can be made effectively on the basis of hydrophilicity, particularly where the biologically functional protein or peptide thereby created is intended for use in immunological embodiments, as in the present case.
- the greatest local average hydrophilicity of a protein as governed by the hydrophilicity of its adjacent amino acids, correlates with its immunogenicity and antigenicity, i.e., with a biological property of the protein.
- hydrophilicity values have been assigned to these amino acid residues: arginine (+3.0); lysine (+3.0); aspartate (+3.0 ⁇ 1); glutamate (+3.0 ⁇ 1); serine (+0.3); asparagine (+0.2); glutamine (+0.2); glycine (0); threonine (- 0.4); proline (-0.5 ⁇ 1); alanine (-0.5); histidine (-0.5); cysteine (-1.0); methionine (-1.3); valine (-1.5); leucine (-1.8); isoleucine (-1.8); tyrosine (-2.3); phenylalanine (-2.5) and tryptophan (-3.4).
- the substitution of amino acids whose hydrophilicity values are within ⁇ 2 is included, in certain embodiments, those which are within ⁇ 1 are included, and in certain embodiments, those within ⁇ 0.5 are included.
- polypeptide structure A skilled artisan will be able to determine suitable variants of the polypeptide as set forth herein using well-known techniques.
- one skilled in the art may identify suitable areas of the molecule that may be changed without destroying activity by targeting regions not believed to be important for activity.
- even areas that may be important for biological activity or for structure may be subject to conservative amino acid substitutions without destroying the biological activity or without adversely affecting the polypeptide structure.
- one skilled in the art can review structure-function studies identifying residues in similar polypeptides that are important for activity or structure. In view of such a comparison, one can predict the importance of amino acid residues in a protein that correspond to amino acid residues which are important for activity or structure in similar proteins. One skilled in the art may opt for chemically similar amino acid substitutions for such predicted important amino acid residues. One skilled in the art can also analyze the three-dimensional structure and amino acid sequence in relation to that structure in similar polypeptides. In view of such information, one skilled in the art may predict the alignment of amino acid residues of an antibody with respect to its three dimensional structure.
- one skilled in the art may choose not to make radical changes to amino acid residues predicted to be on the surface of the protein, since such residues may be involved in important interactions with other molecules.
- one skilled in the art may generate test variants containing a single amino acid substitution at each desired amino acid residue. The variants can then be screened using activity assays known to those skilled in the art. Such variants could be used to gather information about suitable variants. For example, if one discovered that a change to a particular amino acid residue resulted in destroyed, undesirably reduced, or unsuitable activity, variants with such a change may be avoided. In other words, based on information gathered from such routine experiments, one skilled in the art can readily determine the amino acids where further substitutions should be avoided either alone or in combination with other mutations.
- Additional methods of predicting secondary structure include “threading” (Jones, D., Curr. Opin. Struct. Biol, 7(3):377-87 (1997); Sippl et al, Structure, 4(1):15-19 (1996)), “profile analysis” (Bowie et al, Science, 253:164-170 (1991); Gribskov et al, Meth. Enzym., 183:146-159 (1990); Gribskov et al, Proc. Nat. Acad. ScL, 84(13):4355-4358 (1987)), and “evolutionary linkage” (See Holm, supra (1999), and Brenner, supra (1997)).
- antibody variants include glycosylation variants wherein the number and/or type of glycosylation site has been altered compared to the amino acid sequences of the parent polypeptide.
- protein variants comprise a greater or a lesser number of N-linked glycosylation sites than the native protein.
- An N-linked glycosylation site is characterized by the sequence: Asn-X-Ser or Asn-X-Thr, wherein the amino acid residue designated as X may be any amino acid residue except proline.
- the substitution of amino acid residues to create this sequence provides a potential new site for the addition of an N-linked carbohydrate chain. Alternatively, substitutions which eliminate this sequence will remove an existing N-linked carbohydrate chain.
- N-linked carbohydrate chains wherein one or more N-linked glycosylation sites (typically those that are naturally occurring) are eliminated and one or more new N-linked sites are created.
- Additional preferred antibody variants include cysteine variants wherein one or more cysteine residues are deleted from or substituted for another amino acid (e.g., serine) as compared to the parent amino acid sequence. Cysteine variants may be useful when antibodies must be refolded into a biologically active conformation such as after the isolation of insoluble inclusion bodies. Cysteine variants generally have fewer cysteine residues than the native protein, and typically have an even number to minimize interactions resulting from unpaired cysteines.
- amino acid substitutions are those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinities, and/or (5) confer or modify other functional properties on such polypeptides.
- single or multiple amino acid substitutions may be made in the naturally-occurring sequence (in certain embodiments, in the portion of the polypeptide outside the domain(s) forming intermolecular contacts).
- a conservative amino acid substitution typically may not substantially change the structural characteristics of the parent sequence (e.g., a replacement amino acid should not tend to break a helix that occurs in the parent sequence, or disrupt other types of secondary structure that characterizes the parent sequence).
- a replacement amino acid should not tend to break a helix that occurs in the parent sequence, or disrupt other types of secondary structure that characterizes the parent sequence.
- Examples of art-recognized polypeptide secondary and tertiary structures are described in Proteins, Structures and Molecular Principles (Creighton, Ed., W. H. Freeman and Company, New York (1984)); Introduction to Protein Structure (C. Branden and J. Tooze, eds., Garland Publishing, New York, N.Y. (1991)); and Thornton et at. Nature 354:105 (1991).
- the specific binding agent molecules of this invention that are polypeptide or peptide substitution variants may have up to about ten to twelve percent of the original amino acid sequence replaced.
- the heavy chain may have 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid replaced, while the light chain may have 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid replaced.
- the invention also provides derivatives of specific binding agent polypeptides.
- Derivatives include specific binding agent polypeptides bearing modifications other than insertion, deletion, or substitution of amino acid residues.
- the modifications are covalent in nature, and include for example, chemical bonding with polymers, lipids, other organic, and inorganic moieties.
- Derivatives of the invention may be prepared to increase circulating half-life of a specific binding agent polypeptide, or may be designed to improve targeting capacity for the polypeptide to desired cells, tissues, or organs.
- the invention further embraces derivative binding agents covalently modified to include one or more water soluble polymer attachments such as polyethylene glycol, polyoxyethylene glycol, or polypropylene glycol as described U.S.
- Still other useful polymers known in the art include monomethoxy-polyethylene glycol, dextran, cellulose, or other carbohydrate based polymers, poly-(N-vinyl pyrrolidone)-polyethylene glycol, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol) and polyvinyl alcohol, as well as mixtures of these polymers.
- Particularly preferred are specific binding agent products covalently modified with polyethylene glycol (PEG) subunits.
- Water- soluble polymers may be bonded at specific positions, for example at the amino terminus of the specific binding agent products, or randomly attached to one or more side chains of the polypeptide.
- PEG for improving the therapeutic capacity for specific binding agent, and for humanized antibodies in particular, is described in US Patent 6, 133, 426 to Gonzales et al, issued October 17, 2000.
- Ang-2-specific antibody such as the affinity of the antibody for its target.
- These strategies include the use of site-specific or random mutagenesis of the polynucleotide molecule encoding the antibody to generate antibody variants, followed by a screening step designed to recover antibody variants that exhibit the desired change, e.g. increased or decreased affinity.
- amino acid residues most commonly targeted in mutagenic strategies are those in the CDRs. As described supra, these regions contain the residues that actually interact with Ang-2 and other amino acids that affect the spatial arrangement of these residues. However, amino acids in the framework regions of the variable domains outside the CDR regions have also been shown to make substantial contributions to the antigen-binding properties of the antibody, and can be targeted to manipulate such properties. See Hudson, Curr Opin Biotech, 9:395-402 (1999) and references therein.
- Smaller and more effectively screened libraries of antibody variants can be produced by restricting random or site-directed mutagenesis to sites in the CDRs that correspond to areas prone to "hyper-mutation" during the somatic affinity maturation process. See Chowdhury and Pastan, Nature Biotech, 17: 568-572 (1999) and references therein.
- the types of DNA elements known to define hyper-mutation sites in this manner include direct and inverted repeats, certain consensus sequences, secondary structures, and palindromes.
- the consensus DNA sequences include the tetrabase sequence Purine-G-Pyrimidine-A/T (i.e. A or G - G - C or T - A or T) and the serine codon AGY (wherein Y can be a C or a T).
- an embodiment of the present invention includes mutagenic strategies with the goal of increasing the affinity of an antibody for its target. These strategies include mutagenesis of the entire variable heavy and light chain, mutagenesis of the CDR regions only, mutagenesis of the consensus hypermutation sites within the CDRs, mutagenesis of framework regions, or any combination of these approaches ("mutagenesis" in this context could be random or site-directed). Definitive delineation of the CDR regions and identification of residues comprising the binding site of an antibody can be accomplished though solving the structure of the antibody in question, and the antibody-ligand complex, through techniques known to those skilled in the art, such as X-ray crystallography.
- AbM is an integral suite of programs for antibody structure modeling produced by Oxford Molecular Group (Martin et al, Proc Natl Acad Sa : (USA) 86:9268-9272 (1989); Rees, et al, ABMTM, a computer program for modeling variable regions of antibodies, Oxford, UK; Oxford Molecular, Ltd.).
- the AbM suite models the tertiary structure of an antibody from primary sequencing using a combination of knowledge databases and ab initio methods.
- An additional definition known as the contact definition, has been recently introduced. (MacCallum et al, JMoI Biol, 5:732-45 (1996)). This definition is based on an analysis of the available complex crystal structures.
- the CDR regions in the heavy chain are typically referred to as Hl, H2 and H3 and are numbered sequentially in order counting from the amino terminus to the carboxy terminus.
- the CDR regions in the light chain are typically referred to as Ll, L2 and L3 and are numbered sequentially in order counting from the amino terminus to the carboxy terminus.
- the CDR-Hl is approximately 10 to 12 residues in length and typically starts 4 residues after a Cys according to the Chothia and AbM definitions or typically 5 residues later according to the Kabat definition.
- the Hl is typically followed by a Trp, typically Trp-Val, but also Trp-Ile, or Trp-Ala.
- the length of Hl is approximately 10 to 12 residues according to the AbM definition while the Chothia definition excludes the last 4 residues.
- the CDR-H2 typically starts 15 residues after the end of Hl according to the Kabat and AbM definition.
- the residues preceding H2 are typically Leu-Glu- Trp-Ile-Gly but there are a number of variations.
- H2 is typically followed by the amino acid sequence Lys/Arg-Leu/Ile/Val/Phe/Thr/Ala-Thr/Ser/Ile/Ala.
- the length of the H2 is approximately 16 to 19 residues where the AbM definition predicts the length to be typically 9 to 12 residues.
- the CDR-H3 typically starts 33 residues after the end of H2 and is typically preceded by the amino acid sequence (typically Cys-Ala-Arg).
- the H3 is typically followed by the amino acid sequence-Gly.
- the length of H3 can be anywhere between 3 to 25 residues.
- the CDR-Ll typically starts at approximately residue 24 and will typically follow a Cys.
- the residue after the CDR-Ll is always a Trp and will typically begin the sequence Trp-Tyr-Gln, Trp-Leu-Gln, Trp-Phe-Gln, or Trp-Tyr-Leu.
- the length of CDR-Ll is approximately 10 to 17 residues.
- the punitive CDR-Ll for the antibodies of the invention follows this pattern exactly with a Cys residue followed by 15 amino acids then Trp-Tyr-Gln.
- the CDR-L2 starts approximately 16 residues after the end of Ll. It will generally follow residues Ile-Tyr, Val-Tyr, Ile-Lys or Ile-Phe. The length of CDR-L2 is approximately 7 residues.
- the CDR-L3 typically starts 33 residues after the end of L2 and typically follows a Cys.
- L3 is typically followed by the amino acid sequence Phe-Gly- XXX-GIy.
- the length of L3 is approximately 7 to 11 residues.
- each humanized immunoglobulin chain will usually comprise, in addition to the CDRs, amino acids from the donor immunoglobulin framework that are, e.g., capable of interacting with the CDRs to affect binding affinity, such as one or more amino acids which are immediately adjacent to a CDR in the donor immunoglobulin or those within about 3 angstroms as predicted by molecular modeling.
- the heavy and light chains may each be designed by using any one or all of various position criteria.
- the humanized immunoglobulins of the present invention will be substantially non- immunogenic in humans and retain substantially the same affinity as the donor immunoglobulin to the antigen, such as a protein or other compound containing an epitope. See also, related methods in US Patent 5,693,761 to Queen, et al, issued December 2, 1997 ("Polynucleotides encoding improved humanized immunoglobulins”); US Patent 5,693,762 to Queen, et al. , issued December 2, 1997 (“Humanized Immunoglobulins”); US Patent 5,585,089 to Queen, et al. issued December 17, 1996 (“Humanized Immunoglobulins”).
- US Patent 5,565,332 to Hoogenboom et al. issued October 15, 1996 (“Production of chimeric antibodies - a combinatorial approach”) describes methods for the production of antibodies, and antibody fragments which have similar binding specificity as a parent antibody but which have increased human characteristics.
- Humanized antibodies are obtained by chain shuffling, using, for example, phage display technology, and a polypeptide comprising a heavy or light chain variable domain of a non-human antibody specific for an antigen of interest is combined with a repertoire of human complementary (light or heavy) chain variable domains.
- Hybrid pairings that are specific for the antigen of interest are identified and human chains from the selected pairings are combined with a repertoire of human complementary variable domains (heavy or light).
- a component of a CDR from a non-human antibody is combined with a repertoire of component parts of CDRs from human antibodies.
- hybrids are selected and used in a second humanizing shuffling step. Alternatively, this second step is eliminated if the hybrid is already of sufficient human character to be of therapeutic value. Methods of modification to increase human character are also described. See also Winter, FEBS Letts 430:92-92 (1998).
- United States Patent 6,054,297 to Carter et al, issued April 25, 2000 describes a method for making humanized antibodies by substituting a CDR amino acid sequence for the corresponding human CDR amino acid sequence and/or substituting a FR amino acid sequence for the corresponding human FR amino acid sequences.
- US Patent 5,766,886 to Studnicka et al, issued June 16, 1998 (“Modified antibody variable domains") describes methods for identifying the amino acid residues of an antibody variable domain which may be modified without diminishing the native affinity of the antigen binding domain while reducing its immunogenicity with respect to a heterologous species and methods for preparing these modified antibody variable domains which are useful for administration to heterologous species. See also US Patent 5,869,619 to Studnicka issued February 9, 1999.
- humanized antibodies can be modified to eliminate glycosylation sites in order to increase affinity of the antibody for its cognate antigen (Co et al, MoI Immunol 30:1361-1367 (1993)). Techniques such as “reshaping,” “hyperchimerization,” and “veneering/resurfacing” have produced humanized antibodies with greater therapeutic potential. (Vaswami et al, Annals of Allergy, Asthma, & Immunol 81 :105 (1998); Roguska et al, Prot Engineer 9:895-904 (1996)).
- humanizing antibodies results in a loss of antigen binding capacity. It is therefore preferable to "back mutate" the humanized antibody to include one or more of the amino acid residues found in the original (most often rodent) antibody in an attempt to restore binding affinity of the antibody. See, for example, Saldanha et al, MoI Immunol 36:709-19 (1999). Non-Peptide Specific Binding Agent Analogs/Protein Mimetics
- nonpeptide specific binding agent analogs of peptides that provide a stabilized structure or lessened biodegradation, are also contemplated.
- Specific binding agent peptide mimetic analogs can be prepared based on a selected inhibitory peptide by replacement of one or more residues by nonpeptide moieties.
- the nonpeptide moieties permit the peptide to retain its natural confirmation, or stabilize a preferred, e.g., bioactive, confirmation which retains the ability to recognize and bind Ang-2.
- the resulting analog/mimetic exhibits increased binding affinity for Ang-2.
- the specific binding agent peptides of the invention can be modified, for instance, by glycosylation, amidation, carboxylation, or phosphorylation, or by the creation of acid addition salts, amides, esters, in particular C-terminal esters, and N-acyl derivatives of the peptides of the invention.
- the specific binding agent peptides also can be modified to create peptide derivatives by forming covalent or noncovalent complexes with other moieties.
- Covalently-bound complexes can be prepared by linking the chemical moieties to functional groups on the side chains of amino acids comprising the specific binding agent peptides, or at the N- or C- terminus.
- the specific binding agent peptides can be conjugated to a reporter group, including, but not limited to a radiolabel, a fluorescent label, an enzyme (e.g., that catalyzes a colorimetric or fluorometric reaction), a substrate, a solid matrix, or a carrier (e.g., biotin or avidin).
- a reporter group including, but not limited to a radiolabel, a fluorescent label, an enzyme (e.g., that catalyzes a colorimetric or fluorometric reaction), a substrate, a solid matrix, or a carrier (e.g., biotin or avidin).
- the invention accordingly provides a molecule comprising an antibody molecule, wherein the molecule preferably further comprises a reporter group selected from the group consisting of a radiolabel, a fluorescent label, an enzyme, a substrate, a solid matrix, and a carrier.
- Such labels are well known to those of skill in the art, e.g., biotin labels are particularly
- Specific binding agents of the present invention that are proteins can be prepared by chemical synthesis in solution or on a solid support in accordance with conventional techniques.
- the current limit for solid phase synthesis is about 85-100 amino acids in length.
- chemical synthesis techniques can often be used to chemically ligate a series of smaller peptides to generate full length polypeptides.
- Various automatic synthesizers are commercially available and can be used in accordance with known protocols. See, for example, Stewart and Young, Solid Phase Peptide Synthesis, 2d.
- Solid phase peptide synthesis methods use a copoly(styrene- divinylbenzene) containing 0.1-1.0 mM amines/g polymer. These methods for peptide synthesis use butyloxycarbonyl (t-BOC) or 9-fluorenylmethyloxy- carbonyl(FMOC) protection of alpha-amino groups. Both methods involve stepwise syntheses whereby a single amino acid is added at each step starting from the C-terminus of the peptide (See, Coligan et al., Current Protocols in Immunology, Wiley Interscience, 1991, Unit 9).
- the synthetic peptide can be deprotected to remove the t-BOC or FMOC amino acid blocking groups and cleaved from the polymer by treatment with acid at reduced temperature (e.g., liquid HF-10% anisole for about 0.25 to about 1 hours at O 0 C).
- acid at reduced temperature e.g., liquid HF-10% anisole for about 0.25 to about 1 hours at O 0 C.
- the specific binding agent peptides are extracted from the polymer with 1% acetic acid solution that is then lyophilized to yield the crude material. This can normally be purified by such techniques as gel filtration on Sephadex G- 15 using 5% acetic acid as a solvent.
- Lyophilization of appropriate fractions of the column will yield the homogeneous specific binding agent peptide or peptide derivatives, which can then be characterized by such standard techniques as amino acid analysis, thin layer chromatography, high performance liquid chromatography, ultraviolet absorption spectroscopy, molar rotation, solubility, and quantitated by the solid phase Edman degradation.
- Recombinant DNA techniques are a convenient method for preparing full length antibodies and other large proteinaceous specific binding agents of the present invention, or fragments thereof.
- a cDNA molecule encoding the antibody or fragment may be inserted into an expression vector, which can in turn be inserted into a host cell for production of the antibody or fragment. It is understood that the cDNAs encoding such antibodies may be modified to vary from the "original" cDNA (translated from the mRNA) to provide for codon degeneracy or to permit codon preference usage in various host cells.
- a DNA molecule encoding an antibody can be obtained using procedures described herein in the Examples. Where it is desirable to obtain Fab molecules or CDRs that are related to the original antibody molecule, one can screen a suitable library (phage display library; lymphocyte library, etc.) using standard techniques to identify and clone related Fabs/CDRs. Probes used for such screening may be full length or truncated Fab probes encoding the Fab portion of the original antibody, probes against one or more CDRs from the Fab portion of the original antibody, or other suitable probes. Where DNA fragments are used as probes, typical hybridization conditions are those such as set forth in Ausubel et. al.
- the probed blot can be washed at a suitable stringency, depending on such factors as probe size, expected homology of probe to clone, the type of library being screened, and the number of clones being screened. Examples of high stringency screening are 0.1 X SSC, and 0.1 percent SDS at a temperature between 50-65°C.
- a variety of expression vector/host systems may be utilized to contain and express the polynucleotide molecules encoding the specific binding agent polypeptides of the invention.
- These systems include but are not limited to microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with virus expression vectors (e.g., baculo virus); plant cell systems transfected with virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with bacterial expression vectors (e.g., Ti or pBR322 plasmid); or animal cell systems.
- microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with virus expression vectors (e.g., baculo virus); plant cell systems transfected with virus expression vectors
- Mammalian cells that are useful in recombinant specific binding agent protein productions include but are not limited to VERO cells, HeLa cells, Chinese hamster ovary (CHO) cell lines, COS cells (such as COS-7), Wl 38, BHK, HepG2, 3T3, RIN, MDCK, A549, PC12, K562 and 293 cells, as well as hybridoma cell lines as described herein.
- Mammalian cells are preferred for preparation of those specific binding agents such as antibodies and antibody fragments that are typically glycosylated and require proper refolding for activity.
- Preferred mammalian cells include CHO cells, hybridoma cells, and myeloid cells.
- expression vector refers to a plasmid, phage, virus or vector, for expressing a polypeptide from a DNA (RNA) sequence.
- An expression vector can comprise a transcriptional unit comprising an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers, (2) a structural or sequence that encodes the binding agent which is transcribed into mRNA and translated into protein, and (3) appropriate transcription initiation and termination sequences.
- Structural units intended for use in yeast or eukaryotic expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell.
- recombinant specific binding agent protein may include an amino terminal methionine residue. This residue may or may not be subsequently cleaved from the expressed recombinant protein to provide a final specific binding agent product.
- the specific binding agents may be recombinantly expressed in yeast using a commercially available expression system, e.g., the Pichia Expression System (Invitrogen, San Diego, CA), following the manufacturer's instructions. This system also relies on the pre-pro-alpha sequence to direct secretion, but transcription of the insert is driven by the alcohol oxidase (AOXl) promoter upon induction by methanol.
- AOXl alcohol oxidase
- the secreted specific binding agent peptide is purified from the yeast growth medium by, e.g., the methods used to purify the peptide from bacterial and mammalian cell supernatants.
- the cDNA encoding the specific binding agent peptide may be cloned into the baculovirus expression vector pVL1393 (PharMingen, San Diego, CA). This vector can be used according to the manufacturer's directions (PharMingen) to infect Spodoptera frugiperda cells in sF9 protein-free media and to produce recombinant protein.
- the specific binding agent protein can be purified and concentrated from the media using a heparin-Sepharose column (Pharmacia).
- the peptide may be expressed in an insect system.
- Insect systems for protein expression are well known to those of skill in the art.
- Autographa californica nuclear polyhedrosis virus (AcNPV) can be used as a vector to express foreign genes in Spodoptera frugiperda cells or in Trichoplusia larvae.
- the specific binding agent peptide coding sequence can be cloned into a nonessential region of the virus, such as the polyhedrin gene, and placed under control of the polyhedrin promoter. Successful insertion of the specific binding agent peptide will render the polyhedrin gene inactive and produce recombinant virus lacking coat protein coat.
- the recombinant viruses can be used to infect S.
- the DNA sequence encoding the specific binding agent peptide can be amplified by PCR and cloned into an appropriate vector for example, pGEX-3X (Pharmacia).
- the pGEX vector is designed to produce a fusion protein comprising glutathione- S -transferase (GST), encoded by the vector, and a specific binding agent protein encoded by a DNA fragment inserted into the vector's cloning site.
- GST glutathione- S -transferase
- the primers for the PCR can be generated to include for example, an appropriate cleavage site.
- the recombinant specific binding agent fusion protein may then be cleaved from the GST portion of the fusion protein.
- the pGEX-3X/specific binding agent peptide construct is transformed into E. coli XL-I Blue cells (Stratagene, La Jolla CA), and individual transformants isolated and grown. Plasmid DNA from individual transformants can be purified and partially sequenced using an automated sequencer to confirm the presence of the desired specific binding agent encoding nucleic acid insert in the proper orientation.
- Specific binding agents made in bacterial cells may be produced as an insoluble inclusion body in the bacteria, can be purified as follows. Host cells can be sacrificed by centrifugation; washed in 0.15 M NaCl, 10 niM Tris, pH 8, 1 mM EDTA; and treated with 0.1 mg/ml lysozyme (Sigma, St. Louis, MO) for 15 minutes at room temperature. The lysate can be cleared by sonication, and cell debris can be pelleted by centrifugation for 10 minutes at 12,000 X g.
- the specific binding agent-containing pellet can be resuspended in 50 mM Tris, pH 8, and 10 mM EDTA, layered over 50% glycerol, and centrifuged for 30 min. at 6000 X g.
- the pellet can be resuspended in standard phosphate buffered saline solution (PBS) free of Mg ++ and Ca + ⁇
- PBS standard phosphate buffered saline solution
- the specific binding agent can be further purified by fractionating the resuspended pellet in a denaturing SDS polyacrylamide gel (Sambrook et ai, supra). The gel can be soaked in 0.4 M KCl to visualize the protein, which can be excised and electroeluted in gel-running buffer lacking SDS. If the GST fusion protein is produced in bacteria, as a soluble protein, it can be purified using the GST Purification Module (Pharmacia).
- Mammalian host systems for the expression of the -recombinant protein are well known to those of skill in the art.
- Host cell strains can be chosen for a particular ability to process the expressed protein or produce certain post- translation modifications that will be useful in providing protein activity.
- modifications of the polypeptide include, but are not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation.
- Different host cells such as CHO, HeLa, MDCK, 293, WI38, as well as hybridoma cell lines, and the like have specific cellular machinery and characteristic mechanisms for such post-translational activities and can be chosen to ensure the correct modification and processing of the introduced, foreign protein.
- selection systems can be used to recover the cells that have been transformed for recombinant protein production.
- selection systems include, but are not limited to, HSV thymidine kinase, hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase genes, in tk-, hgprt- or aprt- cells, respectively.
- anti-metabolite resistance can be used as the basis of selection for DHFR which confers resistance to methotrexate; gpt which confers resistance to mycophenolic acid; neo which confers resistance to the aminoglycoside G418 and confers resistance to chlorsulfuron; and hygro which that confers resistance to hygromycin.
- Additional selectable genes that may be useful include trpB, which allows cells to utilize indole in place of tryptophan, or hisD, which allows cells to utilize histinol in place of histidine.
- Markers that give a visual indication for identification of transformants include anthocyanins, ⁇ -glucuronidase and its substrate, GUS, and luciferase and its substrate, luciferin.
- the specific binding agents produced using procedures described above may need to be "refolded” and oxidized into a proper tertiary structure and generating di-sulf ⁇ de linkages in order to be biologically active.
- Refolding can be accomplished using a number of procedures well known in the art. Such methods include, for example, exposing the solubilized polypeptide agent to a pH usually above 7 in the presence of a chaotropic agent.
- a chaotrope is similar to the choices used for inclusion body solubilization, however a chaotrope is typically used at a lower concentration.
- An exemplary chaotropic agent is guanidine.
- the refolding/oxidation solution will also contain a reducing agent plus its oxidized form in a specific ratio to generate a particular redox potential which allows for dusykfide shuffling to occur for the formation of cysteine bridges.
- Some commonly used redox couples include cysteine/cystamine, glutathione/dithiobisGSH, cupric chloride, dithiothreitol DTT/dithiane DTT, and 2-mercaptoethanol (bME)/dithio-bME.
- a co-solvent may be used to increase the efficiency of the refolding.
- cosolvents include glycerol, polyethylene glycol of various molecular weights, and arginine.
- Protein purification techniques are well known to those of skill in the art. These techniques involve, at one level, the crude fractionation of the polypeptide and non-polypeptide fractions. Having separated the specific binding agent polypeptide from other proteins, the polypeptide of interest can be further purified using chromatographic and electrophoretic techniques to achieve partial or complete purification (or purification to homogeneity). Analytical methods particularly suited to the preparation of a pure specific binding agent peptide are ion-exchange chromatography, exclusion chromatography; polyacrylamide gel electrophoresis; isoelectric focusing. A particularly efficient method of purifying peptides is fast protein liquid chromatography or even HPLC.
- Certain aspects of the present invention concern the purification, and in particular embodiments, the substantial purification, of an encoded specific binding agent protein or peptide.
- the term "purified specific binding agent protein or peptide" as used herein, is intended to refer to a composition, isolatable from other components, wherein the specific binding agent protein or peptide is purified to any degree relative to its naturally-obtainable state.
- a purified specific binding agent protein or peptide therefore also refers to a specific binding agent protein or peptide, free from the environment in which it may naturally occur.
- purified will refer to a specific binding agent composition that has been subjected to fractionation to remove various other components, and which composition substantially retains its expressed biological activity. Where the term “substantially purified” is used, this designation will refer to a specific binding agent composition in which the specific binding agent protein or peptide forms the major component of the composition, such as constituting about 50%, about 60%, about 70%, about 80%, about 90%, about 95% or more of the proteins in the composition.
- Various methods for quantifying the degree of purification of the specific binding agent will be known to those of skill in the art in light of the present disclosure. These include, for example, determining the specific binding activity of an active fraction, or assessing the amount of specific binding agent polypeptides within a fraction by SDS/PAGE analysis.
- a preferred method for assessing the purity of a specific binding agent fraction is to calculate the binding activity of the fraction, to compare it to the binding activity of the initial extract, and to thus calculate the degree of purification, herein assessed by a "-fold purification number.”
- the actual units used to represent the amount of binding activity will, of course, be dependent upon the particular assay technique chosen to follow the purification and whether or not the expressed specific binding agent protein or peptide exhibits a detectable binding activity.
- Partial purification may be accomplished by using fewer purification steps in combination, or by utilizing different forms of the same general purification scheme. For example, it is appreciated that a cation-exchange column chromatography performed utilizing an HPLC apparatus will generally result in a greater "-fold" purification than the same technique utilizing a low- pressure chromatography system. Methods exhibiting a lower degree of relative purification may have advantages in total recovery of specific binding agent protein product, or in maintaining binding activity of an expressed specific binding agent protein.
- Immunological binding assays typically utilize a capture agent to bind specifically to and often immobilize the analyte target antigen.
- the capture agent is a moiety that specifically binds to the analyte.
- the capture agent is an antibody or fragment thereof that specifically binds Ang-2.
- These immunological binding assays are well known in the art (see, Asai, ed., Methods in Cell Biology, Vol. 37, Antibodies in Cell Biology, Academic Press, Inc., New York (1993)).
- Immunological binding assays frequently utilize a labeling agent that will signal the existence of the bound complex formed by the capture agent and antigen.
- the labeling agent can be one of the molecules comprising the bound complex; i.e.
- the labeling agent can be a third molecule, commonly another antibody, which binds to the bound complex.
- the labeling agent can be, for example, an anti-specific binding agent antibody bearing a label.
- the second antibody, specific for the bound complex may lack a label, but can be bound by a fourth molecule specific to the species of antibodies which the second antibody is a member of.
- the second antibody can be modified with a detectable moiety, such as biotin, which can then be bound by a fourth molecule, such as enzyme-labeled streptavidin.
- Other proteins capable of specifically binding immunoglobulin constant regions, such as protein A or protein G may also be used as the labeling agent.
- binding proteins are normal constituents of the cell walls of streptococcal bacteria and exhibit a strong non-immunogenic reactivity with immunoglobulin constant regions from a variety of species (see, generally Akerstrom, J Immunol, 135:2589-2542 (1985); and Chaubert, Mod Pathol, 10:585-591 (1997)).
- incubation and/or washing steps may be required after each combination of reagents. Incubation steps can vary from about 5 seconds to several hours, preferably from about 5 minutes to about 24 hours.
- the incubation time will depend upon the assay format, analyte, volume of solution, concentrations, and the like.
- the assays will be carried out at ambient temperature, although they can be conducted over a range of temperatures.
- Immunological binding assays can be of the non-competitive type. These assays have an amount of captured analyte that is directly measured.
- the capture agent antibody
- the capture agent can be bound directly to a solid substrate where it is immobilized. These immobilized antibodies then capture (bind to) antigen present in the test sample.
- the protein thus immobilized is then bound to a labeling agent, such as a second antibody having a label.
- the second antibody lacks a label, but can be bound by a labeled antibody specific for antibodies of the species from which the second antibody is derived.
- the second antibody also can be modified with a detectable moiety, such as biotin, to which a third labeled molecule can specifically bind, such as streptavidin.
- a detectable moiety such as biotin
- streptavidin See, Harlow and Lane, Antibodies, A Laboratory Manual, Ch 14, Cold Spring Harbor Laboratory, NY (1988), incorporated herein by reference.
- Immunological binding assays can be of the competitive type.
- the amount of analyte present in the sample is measure indirectly by measuring the amount of an added analyte displaced, or competed away, from a capture agent by the analyte present in the sample.
- a known amount of analyte usually labeled, is added to the sample and the sample is then contacted with an antibody (the capture agent).
- the amount of labeled analyze bound to the antibody is inversely proportional to the concentration of analyte present in the sample. (See, Harlow and Lane, Antibodies, A Laboratory Manual, Ch 14, pp. 579-583, supra).
- the antibody is immobilized on a solid substrate.
- the amount of protein bound to the antibody may be determined either by measuring the amount of protein present in a protein/antibody complex, or alternatively by measuring the amount of remaining uncomplexed protein.
- the amount of protein may be detected by providing a labeled protein. See, Harlow and Lane, Antibodies, A Laboratory Manual, Ch 14, supra).
- hapten inhibition is utilized.
- a known analyte is immobilized on a solid substrate.
- a known amount of antibody is added to the sample, and the sample is contacted with the immobilized analyte.
- the amount of antibody bound to the immobilized analyte is inversely proportional to the amount of analyte present in the sample.
- the amount of immobilized antibody may be detected by detecting either the immobilized fraction of antibody or the fraction that remains in solution. Detection may be direct where the antibody is labeled or indirect by the subsequent addition of a labeled moiety that specifically binds to the antibody as described above.
- the competitive binding assays can be used for cross-reactivity determinations to permit a skilled artisan to determine if a protein or enzyme complex which is recognized by a specific binding agent of the invention is the desired protein and not a cross-reacting molecule or to determine whether the antibody to is specific for the antigen and does not bind unrelated antigens.
- antigen can be immobilized to a solid support and an unknown protein mixture is added to the assay, which will compete with the binding of the specific binding agents to the immobilized protein.
- the competing molecule also binds one or more antigens unrelated to the antigen.
- the present invention also provides Western blot methods to detect or quantify the presence of Ang-2 in a sample.
- the technique generally comprises separating sample proteins by gel electrophoresis on the basis of molecular weight and transferring the proteins to a suitable solid support, such as nitrocellulose filter, a nylon filter, or derivatized nylon filter.
- a suitable solid support such as nitrocellulose filter, a nylon filter, or derivatized nylon filter.
- the sample is incubated with antibodies or fragments thereof that specifically bind Ang-2 and the resulting complex is detected. These antibodies may be directly labeled or alternatively may be subsequently detected using labeled antibodies that specifically bind to the antibody.
- Binding assays to detect those Ang-2 specific binding agents that disrupt Ang-2 binding to its receptor are set forth in the Examples herein.
- the antibodies or fragments thereof of present invention are useful for the diagnosis of conditions or diseases characterized by expression of Ang-2 or subunits, or in assays to monitor patients being treated with inducers of Ang-2, its fragments, agonists or inhibitors of Ang-2 activity.
- Diagnostic assays for Ang-2 include methods utilizing a specific binding agent and a label to detect Ang-2 in human body fluids or extracts of cells or tissues.
- the specific binding agents of the present invention can be used with or without modification.
- the specific binding agents will be labeled by attaching, e.g., a label or a reporter molecule.
- a wide variety of labels and reporter molecules are known, some of which have been already described herein.
- the present invention is useful for diagnosis of human disease.
- Ang-2 proteins A variety of protocols for measuring Ang-2 proteins using either polyclonal or monoclonal antibodies specific for the respective protein are known in the art. Examples include enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and fluorescence activated cell sorting (FACS).
- ELISA enzyme-linked immunosorbent assay
- RIA radioimmunoassay
- FACS fluorescence activated cell sorting
- normal or standard values for human Ang-2 expression are usually established. This determination can be accomplished by combining body fluids or cell extracts from normal subjects, preferably human, with a specific binding agent, for example, an antibody, to Ang-2, under conditions suitable for complex formation that are well known in the art.
- the amount of standard complex formation can be quantified by comparing the binding of the specific binding agents to known quantities of Ang-2 protein, with both control and disease samples. Then, standard values obtained from normal samples can be compared with values obtained from samples from subjects potentially affected by disease. Deviation between standard and subject values suggests a role for Ang-2 in the disease state.
- detectable moiety can be any one that is capable of producing, either directly or indirectly, a detectable signal.
- the detectable moiety may be a radioisotope, such as 3 H, 14 C, 32 P, 35 S, or 125 I, a fluorescent or chemiluminescent compound, such as fluorescein isothiocyanate, rhodamine, or luciferin; or an enzyme, such as alkaline phosphatase, ⁇ -galactosidase, or horseradish peroxidase (Bayer et al, Meth Enz, 184: 138-163, (1990)).
- the present invention provides a specific binding agent that binds to Ang- 2 that is useful for the treatment of human diseases and pathological conditions.
- Agents that modulate Ang-2 binding activity, or other cellular activity, may be used in combination with other therapeutic agents to enhance their therapeutic effects or decrease potential side effects.
- the present invention provides reagents and methods useful for treating diseases and conditions characterized by undesirable or aberrant levels of Ang-2 activity in a cell.
- diseases include cancers, and other hyperproliferative conditions, such as hyperplasia, psoriasis, contact dermatitis, immunological disorders, and infertility.
- the present invention also provides methods of treating cancer in an animal, including humans, comprising administering to the animal an effective amount of a specific binding agent that inhibits or decreases Ang-2 activity.
- the invention is further directed to methods of inhibiting cancer cell growth, including processes of cellular proliferation, invasiveness, and metastasis in biological systems.
- Methods include use of a compound of the invention as an inhibitor of cancer cell growth.
- the methods are employed to inhibit or reduce cancer cell growth, invasiveness, metastasis, or tumor incidence in living animals, such as mammals.
- Methods of the invention are also readily adaptable for use in assay systems, e.g., assaying cancer cell growth and properties thereof, as well as identifying compounds that affect cancer cell growth.
- the cancers treatable by methods of the present invention preferably occur in mammals.
- Mammals include, for example, humans and other primates, as well as pet or companion animals such as dogs and cats, laboratory animals such as rats, mice and rabbits, and farm animals such as horses, pigs, sheep, and cattle.
- Tumors or neoplasms include growths of tissue cells in which the multiplication of the cells is uncontrolled and progressive. Some such growths are benign, but others are termed malignant and may lead to death of the organism. Malignant neoplasms or cancers are distinguished from benign growths in that, in addition to exhibiting aggressive cellular proliferation, they may invade surrounding tissues and metastasize. Moreover, malignant neoplasms are characterized in that they show a greater loss of differentiation (greater dedifferentiation), and of their organization relative to one another and their surrounding tissues. This property is also called "anaplasia.”
- Neoplasms treatable by the present invention also include solid tumors, i.e., carcinomas and sarcomas.
- Carcinomas include those malignant neoplasms derived from epithelial cells that infiltrate (invade) the surrounding tissues and give rise to metastases.
- Adenocarcinomas are carcinomas derived from glandular tissue, or which form recognizable glandular structures.
- Another broad category or cancers includes sarcomas, which are tumors whose cells are embedded in a fibrillar or homogeneous substance like embryonic connective tissue.
- the invention also enables treatment of cancers of the myeloid or lymphoid systems, including leukemias, lymphomas and other cancers that typically do not present as a tumor mass, but are distributed in the vascular or lymphoreticular systems.
- the type of cancer or tumor cells amenable to treatment according to the invention include, for example, ACTH-producing tumor, acute lymphocytic leukemia, acute nonlymphocytic leukemia, cancer of the adrenal cortex, bladder cancer, brain cancer, breast cancer, cervical cancer, chronic lymphocytic leukemia, chronic myelocytic leukemia, colorectal cancer, cutaneous T-cell lymphoma, endometrial cancer, esophageal cancer, Ewing's sarcoma, gallbladder cancer, hairy cell leukemia, head and neck cancer, Hodgkin's lymphoma, Kaposi's sarcoma, kidney cancer, liver cancer, lung cancer (small and non-small cell), malignant peritoneal effusion, malignant pleural effusion, melanoma, mesothelioma, multiple myeloma, neuroblastoma, glioma, non-Hodgkin's lymphoma, osteosarcoma, ova
- the invention is particularly illustrated herein in reference to treatment of certain types of experimentally defined cancers.
- standard state-of-the-art in vitro and in vivo models have been used. These methods can be used to identify agents that can be expected to be efficacious in in vivo treatment regimens.
- the method of the invention is not limited to the treatment of these tumor types, but extends to any solid tumor derived from any organ system. Cancers whose invasiveness or metastasis is associated with Ang-2 expression or activity are especially susceptible to being inhibited or even induced to regress by means of the invention.
- the invention can also be practiced by including with a specific binding agent of the invention, such as an antibody, in combination with another anti-cancer chemotherapeutic agent, such as any conventional chemotherapeutic agent.
- a specific binding agent of the invention such as an antibody
- another anti-cancer chemotherapeutic agent such as any conventional chemotherapeutic agent.
- the combination of a specific binding agent with such other agents can potentiate the chemotherapeutic protocol.
- Numerous chemotherapeutic protocols will present themselves in the mind of the skilled practitioner as being capable of incorporation into the method of the invention. Any chemotherapeutic agent can be used, including alkylating agents, antimetabolites, hormones and antagonists, radioisotopes, as well as natural products.
- the compound of the invention can be administered with antibiotics such as doxorubicin and other anthracycline analogs, nitrogen mustards such as cyclophosphamide, pyrimidine analogs such as 5-fluorouracil, cisplatin, hydroxyurea, taxol and its natural and synthetic derivatives, and the like.
- antibiotics such as doxorubicin and other anthracycline analogs
- nitrogen mustards such as cyclophosphamide
- pyrimidine analogs such as 5-fluorouracil, cisplatin
- hydroxyurea taxol and its natural and synthetic derivatives, and the like.
- the compound in the case of mixed tumors, such as adenocarcinoma of the breast, where the tumors include gonadotropin-dependent and gonadotropin-independent cells
- the compound in conjunction with leuprolide or goserelin (synthetic peptide analogs of LH-RH).
- antineoplastic protocols include the use of a tetracycline compound with another treatment modality, e.g., surgery, radiation, etc., also referred to herein as "adjunct antineoplastic modalities.”
- another treatment modality e.g., surgery, radiation, etc.
- the method of the invention can be employed with such conventional regimens with the benefit of reducing side effects and enhancing efficacy.
- the present invention thus provides compositions and methods useful for the treatment of a wide variety of cancers, including solid tumors and leukemias.
- Types of cancer that may be treated include, but are not limited to: adenocarcinoma of the breast, prostate, and colon; all forms of bronchogenic carcinoma of the lung; myeloid; melanoma; hepatoma; neuroblastoma; papilloma; apudoma; choristoma; branchioma; malignant carcinoid syndrome; carcinoid heart disease; carcinoma (e.g., Walker, basal cell, basosquamous, Brown-Pearce, ductal, Ehrlich tumor, Krebs 2, merkel cell, mucinous, non-small cell lung, oat cell, papillary, scirrhous, bronchiolar, bronchogenic, squamous cell, and transitional cell); histiocytic disorders; leukemia; histiocytosis malignant; Hod
- cancers may also be treated: adenoma; cholangioma; cholesteatoma; cyclindroma; cystadenocarcinoma; cystadenoma; granulosa cell tumor; gynandroblastoma; hepatoma; hidradenoma; islet cell tumor; Leydig cell tumor; papilloma; Sertoli cell tumor; theca cell tumor; leiomyoma; leiomyosarcoma; myoblastoma; myoma; myosarcoma; rhabdomyoma; rhabdomyosarcoma; ependymoma; ganglioneuroma; glioma; medulloblastoma; meningioma; neurilemmoma; neuroblastoma; neuroepithelioma; neurofibroma; neuroma; paraganglioma; paraganglioma
- Another aspect of the present invention is using the materials and methods of the present invention to prevent and/or treat any hyperproliferative condition of the skin including psoriasis and contact dermatitis or other hyperproliferative diseases. It has been demonstrated that patients with psoriasis and contact dermatitis have elevated Ang-2 activity within these lesions (Ogoshi et al, J. Inv.
- Ang-2 will be used in combination with other pharmaceutical agents to treat humans that express these clinical symptoms.
- the specific binding agents can be delivered using any of the various carriers through routes of administration described herein and others that are well known to those of skill in the art.
- retinopathies including diabetic retinopathy and age-related macular degeneration
- angiogenesis is involved
- disorders/diseases of the female reproductive tract such as endometriosis, uterine fibroids, and other such conditions associated with dysfunctional vascular proliferation (including endometrial microvascular growth) during the female reproductive cycle.
- Still another aspect of the present invention relates to treating abnormal vascular growth including cerebral arteriovenous malformations (AVMs) gastrointestinal mucosal injury and repair, ulceration of the gastroduodenal mucosa in patients with a history of peptic ulcer disease, including ischemia resulting from stroke, a wide spectrum of pulmonary vascular disorders in liver disease and portal hypertension in patients with nonhepatic portal hypertension.
- AVMs cerebral arteriovenous malformations
- gastrointestinal mucosal injury and repair ulceration of the gastroduodenal mucosa in patients with a history of peptic ulcer disease, including ischemia resulting from stroke, a wide spectrum of pulmonary vascular disorders in liver disease and portal hypertension in patients with nonhepatic portal hypertension.
- Another aspect of present invention is the prevention of cancers utilizing the compositions and methods provided by the present invention.
- Such reagents will include specific binding agents against Ang-2.
- compositions of Ang-2 specific binding agents are within the scope of the present invention.
- Pharmaceutical compositions comprising antibodies are described in detail in, for example, US Patent 6,171,586, to Lam et al, issued January 9, 2001.
- Such compositions comprise a therapeutically or prophylactically effective amount of a specific binding agent, such as an antibody, or a fragment, variant, derivative or fusion thereof as described herein, in admixture with a pharmaceutically acceptable agent.
- pharmaceutical compositions comprise antagonist specific binding agents that modulate partially or completely at least one biological activity of Ang-2 in admixture with a pharmaceutically acceptable agent.
- the specific binding agents will be sufficiently purified for administration to an animal.
- the pharmaceutical composition may contain formulation materials for modifying, maintaining or preserving, for example, the pH, osmolarity, viscosity, clarity, color, isotonicity, odor, sterility, stability, rate of dissolution or release, adsorption or penetration of the composition.
- formulation materials for modifying, maintaining or preserving for example, the pH, osmolarity, viscosity, clarity, color, isotonicity, odor, sterility, stability, rate of dissolution or release, adsorption or penetration of the composition.
- Suitable formulation materials include, but are not limited to, amino acids (such as glycine, glutamine, asparagine, arginine or lysine); antimicrobials; antioxidants (such as ascorbic acid, sodium sulfite or sodium hydrogen-sulfite); buffers (such as borate, bicarbonate, Tris-HCl, citrates, phosphates, other organic acids); bulking agents (such as mannitol or glycine), chelating agents (such as ethylenediamine tetraacetic acid (EDTA)); complexing agents (such as caffeine, polyvinylpyrrolidone, beta- cyclodextrin or hydroxypropyl-beta-cyclodextrin); fillers; monosaccharides; disaccharides and other carbohydrates (such as glucose, mannose, or dextrins); proteins (such as serum albumin, gelatin or immunoglobulins); coloring; flavoring and diluting agents; emulsifying agents; hydrophil
- PEG polysorbitan esters
- polysorbates such as polysorbate 20, polysorbate 80, triton, tromethamine, lecithin, cholesterol, tyloxapal
- stability enhancing agents sucrose or sorbitol
- tonicity enhancing agents such as alkali metal halides (preferably sodium or potassium chloride, mannitol sorbitol)
- delivery vehicles diluents; excipients and/or pharmaceutical adjuvants.
- compositions will be determined by one skilled in the art depending upon, for example, the intended route of administration, delivery format, and desired dosage. See for example, Remington's Pharmaceutical Sciences, supra. Such compositions may influence the physical state, stability, rate of in vivo release, and rate of in vivo clearance of the specific binding agent.
- the primary vehicle or carrier in a pharmaceutical composition may be either aqueous or non-aqueous in nature.
- a suitable vehicle or carrier may be water for injection, physiological saline solution or artificial cerebrospinal fluid, possibly supplemented with other materials common in compositions for parenteral administration.
- Neutral buffered saline or saline mixed with serum albumin are further exemplary vehicles.
- Other exemplary pharmaceutical compositions comprise Tris buffer of about pH 7.0-8.5, or acetate buffer of about pH 4.0-5.5, which may further include sorbitol or a suitable substitute therefore.
- binding agent compositions may be prepared for storage by mixing the selected composition having the desired degree of purity with optional formulation agents (Remington's Pharmaceutical Sciences, supra) in the form of a lyophilized cake or an aqueous solution. Further, the binding agent product may be formulated as a lyophilizate using appropriate excipients such as sucrose.
- compositions can be selected for parenteral delivery.
- compositions may be selected for inhalation or for enteral delivery such as orally, aurally, opthalmically, rectally, or vaginally.
- enteral delivery such as orally, aurally, opthalmically, rectally, or vaginally.
- preparation of such pharmaceutically acceptable compositions is within the skill of the art.
- the formulation components are present in concentrations that are acceptable to the site of administration.
- buffers are used to maintain the composition at physiological pH or at slightly lower pH, typically within a pH range of from about 5 to about 8.
- the therapeutic compositions for use in this invention may be in the form of a pyrogen-free, parenterally acceptable aqueous solution comprising the desired specific binding agent in a pharmaceutically acceptable vehicle.
- a particularly suitable vehicle for parenteral injection is sterile distilled water in which a binding agent is formulated as a sterile, isotonic solution, properly preserved.
- Yet another preparation can involve the formulation of the desired molecule with an agent, such as injectable microspheres, bio-erodible particles, polymeric compounds (polylactic acid, polyglycolic acid), beads, or liposomes, that provides for the controlled or sustained release of the product which may then be delivered via a depot injection.
- Hyaluronic acid may also be used, and this may have the effect of promoting sustained duration in the circulation.
- Other suitable means for the introduction of the desired molecule include implantable drug delivery devices.
- compositions suitable for parenteral administration may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks' solution, ringer's solution, or physiologically buffered saline.
- Aqueous injection suspensions may contain substances that increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran.
- suspensions of the active compounds may be prepared as appropriate oily injection suspensions.
- Suitable lipophilic solvents or vehicles include fatty oils, such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate, triglycerides, or liposomes.
- Non ⁇ lipid polycationic amino polymers may also be used for delivery.
- the suspension may also contain suitable stabilizers or agents to increase the solubility of the compounds and allow for the preparation of highly concentrated solutions.
- a pharmaceutical composition may be formulated for inhalation.
- a binding agent may be formulated as a dry powder for inhalation.
- Polypeptide or nucleic acid molecule inhalation solutions may also be formulated with a propellant for aerosol delivery.
- solutions may be nebulized. Pulmonary administration is further described in PCT Application No. PCT/US94/001875, which describes pulmonary delivery of chemically modified proteins.
- binding agent molecules that are administered in this fashion can be formulated with or without those carriers customarily used in the compounding of solid dosage forms such as tablets and capsules.
- a capsule may be designed to release the active portion of the formulation at the point in the gastrointestinal tract when bioavailability is maximized and pre-systemic degradation is minimized.
- Additional agents can be included to facilitate absorption of the binding agent molecule. Diluents, flavorings, low melting point waxes, vegetable oils, lubricants, suspending agents, tablet disintegrating agents, and binders may also be employed.
- compositions for oral administration can also be formulated using pharmaceutically acceptable carriers well known in the art in dosages suitable for oral administration.
- Such carriers enable the pharmaceutical compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for ingestion by the patient.
- compositions for oral use can be obtained through combining active compounds with solid excipient and processing the resultant mixture of granules (optionally, after grinding) to obtain tablets or dragee cores.
- auxiliaries can be added, if desired.
- Suitable excipients include carbohydrate or protein fillers, such as sugars, including lactose, sucrose, mannitol, and sorbitol; starch from corn, wheat, rice, potato, or other plants; cellulose, such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium carboxymethylcellulose; gums, including arabic and tragacanth; and proteins, such as gelatin and collagen.
- disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, and alginic acid or a salt thereof, such as sodium alginate.
- Dragee cores may be used in conjunction with suitable coatings, such as concentrated sugar solutions, which may also contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures.
- suitable coatings such as concentrated sugar solutions, which may also contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures.
- Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterize the quantity of active compound, i.e., dosage.
- compositions that can be used orally also include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating, such as glycerol or sorbitol.
- Push-fit capsules can contain active ingredients mixed with fillers or binders, such as lactose or starches, lubricants, such as talc or magnesium stearate, and, optionally, stabilizers.
- the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid, or liquid polyethylene glycol with or without stabilizers.
- Another pharmaceutical composition may involve an effective quantity of binding agent in a mixture with non-toxic excipients that are suitable for the manufacture of tablets.
- Suitable excipients include, but are not limited to, inert diluents, such as calcium carbonate, sodium carbonate or bicarbonate, lactose, or calcium phosphate; or binding agents, such as starch, gelatin, or acacia; or lubricating agents such as magnesium ' stearate, stearic acid, or talc.
- sustained-release preparations include semipermeable polymer matrices in the form of shaped articles, e.g. films, or microcapsules. Sustained release matrices may include polyesters, hydrogels, polylactides (U.S.
- Sustained-release compositions also include liposomes, which can be prepared by any of several methods known in the art. See e.g., Eppstein et ah, Proc Natl Acad Sci (USA), 82:3688-3692 (1985); EP 36,676; EP 88,046; EP 143,949.
- the pharmaceutical composition to be used for in vivo administration typically must be sterile. This may be accomplished by filtration through sterile filtration membranes. Where the composition is lyophilized, sterilization using this method may be conducted either prior to or following lyophilization and reconstitution.
- the composition for parenteral administration may be stored in lyophilized form or in solution.
- parenteral compositions generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having a stopper pierceable by a hypodermic injection needle.
- a sterile access port for example, an intravenous solution bag or vial having a stopper pierceable by a hypodermic injection needle.
- the pharmaceutical composition may be stored in sterile vials as a solution, suspension, gel, emulsion, solid, or a dehydrated or lyophilized powder.
- Such formulations may be stored either in a ready-to-use form or in a form (e.g., lyophilized) requiring reconstitution prior to administration.
- kits for producing a single-dose administration unit may each contain both a first container having a dried protein and a second container having an aqueous formulation. Also included within the scope of this invention are kits containing single and multi-chambered pre-filled syringes (e.g., liquid syringes and lyosyringes).
- An effective amount of a pharmaceutical composition to be employed therapeutically will depend, for example, upon the therapeutic context and objectives.
- a typical dosage may range from about 0.1 mg/kg to up to about 100 mg/kg or more, depending on the factors mentioned above. In other embodiments, the dosage may range from 0.1 mg/kg up to about 100 mg/kg; or 1 mg/kg up to about 100 mg/kg; or 5 mg/kg up to about 100 mg/kg.
- the therapeutically effective dose can be estimated initially either in cell culture assays or in animal models such as mice, rats, rabbits, dogs, or pigs.
- animal models such as mice, rats, rabbits, dogs, or pigs.
- An animal model may also be used to determine the appropriate concentration range and route of administration. Such information can then be used to determine useful doses and routes for administration in humans.
- Dosage and administration are adjusted to provide sufficient levels of the active compound or to maintain the desired effect. Factors that may be taken into account include the severity of the disease state, the general health of the subject, the age, weight, and gender of the subject, time and frequency of administration, drug combination(s), reaction sensitivities, and response to therapy. Long-acting pharmaceutical compositions may be administered every 3 to 4 days, every week, or biweekly depending on the half- life and clearance rate of the particular formulation.
- the frequency of dosing will depend upon the pharmacokinetic parameters of the binding agent molecule in the formulation used.
- a composition is administered until a dosage is reached that achieves the desired effect.
- the composition may therefore be administered as a single dose, or as multiple doses (at the same or different concentrations/dosages) over time, or as a continuous infusion. Further refinement of the appropriate dosage is routinely made. Appropriate dosages may be ascertained through use of appropriate dose-response data.
- the route of administration of the pharmaceutical composition is in accord with known methods, e.g.
- compositions may be administered by bolus injection or continuously by infusion, or by implantation device.
- the composition may be administered locally via implantation of a membrane, sponge, or another appropriate material on to which the desired molecule has been absorbed or encapsulated.
- a membrane, sponge, or another appropriate material on to which the desired molecule has been absorbed or encapsulated may be used.
- the device may be implanted into any suitable tissue or organ, and delivery of the desired molecule may be via diffusion, timed-release bolus, or continuous administration.
- compositions in an ex vivo manner.
- cells, tissues, or organs that have been removed from the patient are exposed to the pharmaceutical compositions after which the cells, tissues and/or organs are subsequently implanted back into the patient.
- a binding agent which is a polypeptide can be delivered by implanting certain cells that have been genetically engineered, using methods such as those described herein, to express and secrete the polypeptide.
- Such cells may be animal or human cells, and may be autologous, heterologous, or xenogeneic.
- the cells may be immortalized.
- the cells may be encapsulated to avoid infiltration of surrounding tissues.
- the encapsulation materials are typically biocompatible, semi-permeable polymeric enclosures or membranes that allow the release of the protein product(s) but prevent the destruction of the cells by the patient's immune system or by other detrimental factors from the surrounding tissues.
- Specific binding agents of the invention can be utilized in combination with other therapeutic in the treatment of Ang-2 pathologies. These other therapeutics include, for example radiation treatment, chemotherapy, and targeted therapies as described herein below. Additional combination therapies not specifically listed herein are also within the scope of the present invention.
- the invention thus includes administration of one or more specific binding agent of the invention administered to the same patient in combination with one or more additionally suitable agent(s), each being administered according to a regimen suitable for that medicament. This includes concurrent administration of a specific binding agent of the invention and one or more suitable agents.
- the terms “concurrently administered” and “concurrent administration” encompass substantially simultaneous administration of one or more specific binding agent(s) according to the invention and one or more additionally suitable agents(s).
- non-concurrent administration encompasses administering one or more selective binding agent according to the invention and one or more additionally suitable agent(s), at different times, in any order, whether overlapping or not.
- Components may be administered in the same or in separate compositions, and by the same or different routes of administration.
- the combination therapy comprises a specific binding agent capable of binding Ang-2, in combination with at least one additional anti-angiogenic agent.
- Agents include, but are not limited to, in vitro synthetically prepared chemical compositions, antibodies, antigen binding regions, radionuclides, and combinations and conjugates thereof.
- an agent may act as an agonist, antagonist, alllosteric modulator, or toxin.
- an agent may act to inhibit or stimulate its target (e.g., receptor or enzyme activation or inhibition), and thereby promote cell death or arrest cell growth.
- Chemotherapy treatment can employ anti-neoplastic agents including, for example, alkylating agents including: nitrogen mustards, such as mechlorethamine, cyclophosphamide, ifosfamide, melphalan and chlorambucil; nitrosoureas, such as carmustine (BCNU), lomustine (CCNU), and semustine (methyl-CCNU); ethylenimines/methylmelamine such as thriethylenemelamine (TEM), triethylene, thiophosphamide (thiotepa), hexamethylmelamine (HMM, altretamine); alkyl sulfonates such as busulfan; triazines such as dacarbazine (DTIC); antimetabolites including folic acid analogs such as methotrexate and trimetrexate, pyrimidine analogs such as 5-fluorouracil, fluorodeoxyuridine, gemcitabine, cytosine arabinoside (AraC,
- Cancer therapies which may be administered with a specific binding agent to Ang-2, also include, but are not limited to, targeted therapies as described herein.
- targeted therapies include, but are not limited to, use of therapeutic antibodies.
- Exemplary therapeutic antibodies include, but are not limited to, mouse, mouse-human chimeric, CDR-grafted, humanized and fully human antibodies, and synthetic antibodies, including, but not limited to, those selected by screening antibody libraries.
- Exemplary antibodies include, but are not limited to, those which bind to cell surface proteins Her2, CDC20, CDC33, mucin-like glycoprotein, and epidermal growth factor receptor (EGFR) present on tumor cells, and optionally induce a cytostatic and/or cytotoxic effect on tumor cells displaying these proteins.
- Her2, CDC20, CDC33 mucin-like glycoprotein
- EGFR epidermal growth factor receptor
- Exemplary antibodies also include HERCEPTINTM (trastuzumab), which may be used to treat breast cancer and other forms of cancer, and RITUXANTM (rituximab), ZEVALINTM (ibritumomab tiuxetan), GLEEVECTM, and LYMPHOCIDETM (epratuzumab), which may be used to treat non-Hodgkin's lymphoma and other forms of cancer.
- HERCEPTINTM trastuzumab
- RITUXANTM rituximab
- ZEVALINTM ibritumomab tiuxetan
- GLEEVECTM GLEEVECTM
- LYMPHOCIDETM epratuzumab
- Certain exemplary antibodies also include ERBITUXTM (IMC-C225); IRESSATM (ertinolib) ; BEXXARTM (iodine 131 tositumomab); KDR (kinase domain receptor) inhibitors; anti-VEGF antibodies and antagonists (e.g., AVASTINTM and VEGAF-TRAP); anti-VEGF receptor antibodies and antigen binding regions; anti-Ang-1 antibodies and antigen binding regions; antibodies to Tie-2 and other Ang-1 and Ang-2 receptors; Tie-2 ligands; antibodies against Tie-2 kinase inhibitors; and Campath ® (Alemtuzumab).
- ERBITUXTM IMC-C225
- IRESSATM ertinolib
- BEXXARTM iodine 131 tositumomab
- KDR kinase domain receptor
- anti-VEGF antibodies and antagonists e.g., AVASTINTM and VEGAF-TRA
- cancer therapy agents are polypeptides which selectively induce apoptosis in tumor cells, including, but not limited to, TNF-related polypeptides, such as TRAIL (TNF Receptor Apoptosis-Inducing Ligand).
- TNF-related polypeptides such as TRAIL (TNF Receptor Apoptosis-Inducing Ligand).
- suitable cancer therapy agents are known to be anti-angiogenic.
- Certain such agents include, but are not limited to, IL-8; CampathTM, B-FGF; FGF antagonists; Tek antagonists (Cerretti et al. , U.S. Publication No. 2003/0162712; Cerretti et al., U.S. Patent No. 6,413,932, and Cerretti et al., U.S. Patent No. 6,521,424, each of which is incorporated herein by reference for any purpose); anti-TWEAK agents (which include, but are not limited to, antibodies and antigen binding regions); soluble TWEAK receptor antagonists (Wiley, U.S. Patent No.
- ADAM distintegrins or domains thereof to antagonize the binding of integrin to its ligands (Fanslow et al., U.S. Publication No. 2002/0042368); anti-eph receptor and anti-ephrin antibodies; antigen binding regions, or antagonists (U.S. Patent Nos. 5,981,245; 5,728,813; 5,969,110; 6,596,852; 6,232,447; 6,057,124 and patent family members thereof); anti-VEGF agents as described herein (e.g.
- antibodies or antigen binding regions that specifically bind VEGF, or soluble VEGF receptors or a ligand binding regions thereof) such as AVASTINTM or VEGF-TRAPTM, and anti-VEGF receptor agents (e.g., antibodies or antigen binding regions that specifically bind thereto), EGFR inhibitory agents (e.g., antibodies or antigen binding regions that specifically bind thereto) such as panitumumab, IRESSATM (gefitinib), TARCEV ATM (erlotinib), anti-Ang-1 and anti-Ang-2 agents (e.g., antibodies or antigen binding regions specifically binding thereto or to their receptors, e.g., Tie- 2/TEK), and anti-Tie-2 kinase inhibitory agents (e.g., antibodies or antigen binding regions that specifically bind and inhibit the activity of growth factors, such as antagonists of hepatocyte growth factor (HGF, also known as Scatter Factor), and antibodies or antigen binding regions that specifically bind its receptor "c
- cancer therapy agents are angiogenesis inhibitors.
- Certain such inhibitors include, but are not limited to, SD-7784 (Pfizer, USA); cilengitide.(Merck KGaA, Germany, EPO 770622); pegaptanib octasodium, (Gilead Sciences, USA); Alphastatin, (BioActa, UK); M-PGA, (Celgene, USA, US 5712291); ilomastat, (Arriva, USA, US 5892112); semaxanib, (Pfizer, USA, US 5792783); vatalanib, (Novartis, Switzerland); 2-methoxyestradiol, (EntreMed, USA); TLC ELL-12, (Elan, Ireland); anecortave acetate, (Alcon, USA); alpha- D148 Mab, (Amgen, USA); CEP-7055,(Cephalon, USA); anti-Vn Mab, (Crucell, Netherlands) DAC:
- AVE 8062 ((2S)-2-amino-3-hydroxy-N-[2-methoxy-5-[(lZ)- 2-(3,4,5-trimethoxyphenyl)ethenyl]p henyljpropanamide monohydrochloride); metelimumab (pINN)(irnmunoglobulin G4, anti-(human transforming growth factor .beta.l (human monoclonal CAT 192 .gamma.4-chain)), disulfide with human monoclonal CAT 192 .kappa.-chain dimer); Flt3 ligand; CD40 ligand; interleukin-2; interleukin-12; 4-1BB ligand; anti-4-lBB antibodies; TNF antagonists and TNF receptor antagonists including TNFR/Fc, TWEAK antagonists and TWEAK-R antagonists including TWEAK-R/Fc; TRAIL; VEGF antagonists including anti-VEGF antibodies; VEGF receptor (including
- Ang-2 thrombospondin 1 inhibitor
- inhibitors of one or both of Tie-2 or Tie-2 ligands such as Ang-2
- a number of inhibitors of Ang-2 are known in the art, including certain anti-Ang-2 antibodies described in published U.S. Patent Application No. 20030124129 (corresponding to PCT Application No. WO03/030833), and U.S. Patent No. 6,166,185, the contents of which are hereby incorporated by reference in their entirety.
- Ang-2 peptibodies are also known in the art, and can be found in, for example, published U.S. Patent Application No.
- Certain cancer therapy agents include, but are not limited to: thalidomide and thalidomide analogues (N-(2,6-dioxo-3-piperidyl)phthalimide); tecogalan sodium (sulfated polysaccharide peptidoglycan); TAN 1120 (8-acetyl-7,8,9,10- tetrahydro-6,8,l l-trihydroxy-l-methoxy-10-[[octahydro-5-hydroxy-2-(2- hydroxypropyl)-4, 10-dimethylpyrano [3 ,4-d] - 1 ,3 ,6-dioxazocin-8-yl] oxy] -5 ,12- naphthacenedione); suradista (7,7'-[carbonylbis
- Combination therapy with growth factors can include cytokines, lymphokines, growth factors, or other hematopoietic factors such as M-CSF, GM- CSF, TNF, IL-I, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-IO, IL-11, IL- 12, IL-13, IL-14, IL-15, IL-16, IL-17, IL-18, IFN, TNFO, TNFl, TNF2, G-CSF, Meg-CSF, GM-CSF, thrombopoietin, stem cell factor, and erythropoietin.
- compositions can include known angiopoietins, for example Ang-1, -2, -4, -Y, and/or the human Ang-like polypeptide, and/or vascular endothelial growth factor (VEGF).
- Growth factors include angiogenin, bone morphogenic protein- 1, bone morphogenic protein-2, bone morphogenic protein-3, bone morphogenic protein- 4, bone morphogenic protein-5, bone morphogenic protein-6, bone morphogenic protein-7, bone morphogenic protein-8, bone morphogenic protein-9, bone morphogenic protein- 10, bone morphogenic protein- 11, bone morphogenic protein-12, bone morphogenic protein-13, bone morphogenic protein-14, bone morphogenic protein- 15, bone morphogenic protein receptor-IA, bone morphogenic protein receptor IB, brain derived neurotrophic factor, ciliary neutrophic factor, ciliary neutrophic factor receptor, cytokine-induced neutrophil chemotactic factor- 1, cytokine-induced neutrophil, chemotactic factor-2, cyto
- anti-inflammatory agent refers generally to any agent that reduces inflammation or swelling in a patient.
- a number of exemplary anti-inflammatory agents are recited herein, but it will be appreciated that there may be additional suitable anti-inflammatory agents not specifically recited herein, but which are encompassed by the present invention.
- the anti-inflammatory agent can be, for example, a compound that inhibits the interaction of inflammatory cytokines with their receptors.
- cytokine inhibitors useful in combination with the specific binding agents of the invention include, for example, antagonists (such as antibodies) of TGF- ⁇ , as well as antagonists (such as antibodies) directed against interleukins involved in inflammation.
- interleukins are described herein and preferably include, but are not limited to, IL-I, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12, IL- 13, IL-17, and IL-18. See Feghali, et al., Frontiers in Biosci., 2:12-26 (1997).
- Specific binding agents of the invention also may be administered in combination with inhibitors of Protein Kinase A Type 1 to enhance T cell proliferation in HIV-infected patients who are receiving anti-retroviral therapy.
- Nerve growth factors also can be combined with the specific binding agents of the invention to treat certain conditions.
- Such conditions include neurodegenerative diseases, spinal cord injury and multiple sclerosis.
- Other conditions treatable with this combination are glaucoma and diabetes.
- a preferred combination therapy relates to a specific binding agent of the invention administered to a patient in combination with one or more suitable IL-I inhibitor.
- Inhibitors of IL-I include, but are not limited to, receptor-binding peptide fragments of IL-I, antibodies directed against IL-I or IL-I beta or IL-I receptor type I, and recombinant proteins comprising all or portions of receptors for IL-I or modified variants thereof, including genetically-modified muteins, multimeric forms and sustained-release formulations.
- IL- Ira polypeptides include IL-I beta converting enzyme (ICE) inhibitors, antagonistic type I IL-I receptor antibodies, IL-I binding forms of type I IL-I receptor and type II IL-I receptor, antibodies to IL-I, including IL-I alpha and IL-I beta and other IL-I family members, and a therapeutic known as IL-I Trap (Regeneron).
- IL- Ira polypeptides include the forms of IL- Ira described in US Patent No. 5,075,222 and modified forms and variants including those described in U.S. 5,922,573, WO 91/17184, WO 92 16221, and WO 96 09323.
- IL-I beta converting enzyme (ICE) inhibitors include peptidyl and small molecule ICE inhibitors including those described in PCT patent applications WO 91/15577; WO 93/05071; WO 93/09135; WO 93/14777 and WO 93/16710; and European patent application 0 547 699.
- Non-peptidyl compounds include those described in PCT patent application WO 95/26958, U.S. Patent No. 5,552,400, U.S. Patent No. 6,121,266, and Dolle et al., J. Med. Chem., 39, pp. 2438-2440 (1996). Additional ICE inhibitors are described in U.S. Pat. Nos.
- IL-I binding forms of Type I IL-I receptor and type II IL-I receptor are described in U.S. Patent Nos. 4,968,607, 4,968,607, 5,081,228, Re 35,450, 5,319,071, and 5,350,683.
- suitable IL-I antagonists include, but are not limited to, peptides derived from IL-I that are capable of binding competitively to the IL-I signaling receptor, IL-I R type I. Additional guidance regarding certain IL-I (and other cytokine) antagonists can be found in U.S. Patent No. 6,472,179.
- TNF inhibitors are suitable, and include, but are not limited to, receptor-binding peptide fragments of TNF ⁇ , antisense oligonucleotides or ribozymes that inhibit TNF ⁇ production, antibodies directed against TNF ⁇ , and recombinant proteins comprising all or portions of receptors for TNF ⁇ or modified variants thereof, including genetically-modified muteins, multimeric forms and sustained-release formulations.
- TACE Tumor Necrosis Factor- ⁇ Converting Enzyme
- TAPI Interleukinogen activator
- GW-3333X Gaxo Wellcome Inc.
- Suitable molecules that inhibit the formation of the IgA- ⁇ jAT complex, such as the peptides disclosed in EP 0 614 464 B, or antibodies against this complex.
- suitable molecules include, but are not limited to, TNF ⁇ -inhibiting disaccharides, sulfated derivatives of glucosamine, or other similar carbohydrates described in U.S. Patent No. 6,020,323.
- Further suitable molecules include peptide TNF ⁇ inhibitors disclosed in U.S. Patent Nos. 5,641,751 and 5,519,000, and the D-amino acid- containing peptides described in U.S. Patent No. 5,753,628.
- inhibitors of TNF ⁇ converting enzyme are also suitable.
- WO 01/03719 describes further additional agents which can be used in combination in accordance with the invention.
- Still further suitable compounds include, but are not limited to, small molecules such as thalidomide or thalidomide analogs, pentoxifylline, or matrix metalloproteinase (MMP) inhibitors or other small molecules.
- MMP inhibitors for this purpose include, for example, those described in U.S. Patent Nos. 5,883,131, 5,863,949 and 5,861,510 as well as mercapto alkyl peptidyl compounds as described in U.S. Patent No. 5,872,146.
- Other small molecules capable of reducing TNF ⁇ production include, for example, the molecules described in U.S. Patent Nos.
- Additional suitable small molecules include, but are not limited to, MMP inhibitors as described in U.S. Patent Nos. 5,747,514, and 5,691,382, as well as hydroxamic acid derivatives such as those described in U.S. Patent No. 5,821,262.
- Further suitable molecules include, for example, small molecules that inhibit phosphodiesterase IV and TNF ⁇ production, such as substituted oxime derivatives (WO 96/00215), quinoline sulfonamides (U.S. Patent No. 5,834,485), aryl furan derivatives (WO 99/18095) and heterobicyclic derivatives (WO 96/01825 ;
- GB 2 291 422 A Also useful are thiazole derivatives that suppress TNF ⁇ and IFN ⁇ (WO 99/15524), as well as xanthine derivatives that suppress TNF ⁇ and other proinflammatory cytokines (see, for example, U.S. Patent Nos. 5,118,500, 5,096,906 and 5,196,430). Additional small molecules useful for treating the hereindescribed conditions include those disclosed in U.S. Patent No. 5,547,979.
- analgesics e.g., acetaminophen, codeine, propoxyphene napsylate, oxycodone hydrochloride, hydrocodone bitartrate, tramadol
- corticosteroids e.g., acetaminophen, codeine, propoxyphene napsylate, oxycodone hydrochloride, hydrocodone bitart
- Exemplary Disease-Modifying Anti-Rheumatic Drugs include, but are not limited to: RheumatrexTM (methotrexate); Enbrel® (etanercept); Remicade® (infliximab); Humira rM (adalimumab); Segard® (afelimomab); AravaTM (leflunomide); KineretTM (anakinra); AravaTM (leflunomide); D-penicillamine; Myochrysine; Plaquenil; RidauraTM (auranofin); Solganal; lenercept (Hoffman-La Roche); CDP870 (Celltech); CDP571 (Celltech), as well as the antibodies described in EP 0 516 785 Bl, U.S.
- RheumatrexTM metalhotrexate
- Enbrel® etanercept
- Remicade® infliximab
- Humira rM adalimumab
- suitable molecules include, for example, soluble TNFRs derived from the extracellular regions of TNF ⁇ receptor molecules other than the p55 and p75 TNFRs, such as for example the TNFR described in WO 99/04001, including TNFR-Ig' s derived from this TNFR.
- Additional suitable TNF ⁇ inhibitors are suitable for use as described herein. These include the use not only of an antibody against TNF ⁇ or TNFR as described herein, but also a TNF ⁇ -derived peptide that can act as a competitive inhibitor of TNF ⁇ (such as those described in U.S. Patent No. 5,795,859 or U.S. Patent No.
- TNFR-IgG fusion proteins such as one containing the extracellular portion of the p55 TNF ⁇ receptor, a soluble TNFR other than an IgG fusion protein, or other molecules that reduce endogenous TNF ⁇ levels, such as inhibitors of the TNF ⁇ converting enzyme (see e.g., U.S. 5,594,106), or small molecules or TNF ⁇ inhibitors, a number of which are described herein.
- one exemplary preferred dose range for an antibody against TNF ⁇ is 0.1 to 20 mg/kg, and more preferably is 1-10 mg/kg.
- Another preferred dose range for anti-TNF ⁇ antibody is 0.75 to 7.5 mg/kg of body weight.
- the present invention can also utilize a specific binding agent and any of one or more Non-Steroidal Anti-Inflammatory Drugs (NSAIDs).
- NSAIDs owe their anti-inflammatory action, at least in part, to the inhibition of prostaglandin synthesis. Goodman and Gilman, The Pharmacological Basis of Therapeutics, MacMillan 7th Edition (1985).
- NSAIDs can be characterized into nine groups: (1) salicylic acid derivatives; (2) propionic acid derivatives; (3) acetic acid derivatives; (4) fenamic acid derivatives; (5) carboxylic acid derivatives; (6) butyric acid derivatives; (7) oxicams; (8) pyrazoles and (9) pyrazolones.
- NSAIDs include, but are not limited to: AnaproxTM, Anaprox DSTM (naproxen sodium); AnsaidTM (flurbiprofen); ArthrotecTM (diclofenac sodium + misoprostil); CataflamTM/VoltarenTM (diclofenac potassium); ClinorilTM (sulindac); DayproTM (oxaprozin); DisalcidTM (salsalate); DolobidTM (diflunisal);
- EC Naprosyn naproxen sodium
- Feldene piroxicam
- Indocin Indocin SRTM (indomethacin); LodineTM, Lodine XLTM (etodolac); MotrinTM (ibuprofen); Naprelan (naproxen); Naprosyn (naproxen); Orudis , (ketoprofen); Oruvail (ketoprofen); Relafen (nabumetone); Tolectin , (tolmetin sodium); TrilisateTM (choline magnesium trisalicylate); Cox-1 inhibitors; Cox-2 Inhibitors such as VioxxTM (rofecoxib); Arcoxia tm (etoricoxib), CelebrexTM (celecoxib); MobicTM (meloxicam); BextraTM (valdecoxib), DynastatTM paracoxib sodium; PrexigeTM (lumiracoxib), and nambumetone.
- VioxxTM rof
- NSAIDs include, but are not limited to, the following: ⁇ -acetamidocaproic acid, S-adenosylmethionine, 3-amino-4-hydroxybutyric acid, amixetrine, anitrazafen, antrafenine, bendazac, bendazac lysinate, benzydamine, beprozin, broperamole, bucolome, bufezolac, ciproquazone, cloximate, dazidamine, deboxamet, detomidine, difenpiramide, difenpyramide, difisalamine, ditazol, emorfazone, fanetizole mesylate, fenflumizole, floctafenine, flumizole, flunixin, fluproquazone, fopirtoline, fosfosal, guaimesal, guaiazolene, isonixirn, lefetamine HCl, leflunomide
- Suitable SAARDs or DMARDS include, but are not limited to: allocupreide sodium, auranofin, aurothioglucose, aurothioglycanide, azathioprine, brequinar sodium, bucillamine, calcium 3-aurothio-2-propanol-l -sulfonate, chlorambucil, chloroquine, clobuzarit, cuproxoline, cyclophosphamide, cyclosporin, dapsone, 15-deoxyspergualin, diacerein, glucosamine, gold salts (e.g., cycloquine gold salt, gold sodium thiomalate, gold sodium thiosulfate), hydroxychloroquine, hydroxyurea, kebuzone, levamisole, lobenzarit, melittin, 6- mercaptopurine, methotrexate, mizoribine, mycophenolate mofetil, myoral, nitrogen mustard,
- Inhibitors of kinases in signaling cascades are also suitable agents for combination with the specific binding agents of the invention. These include, but are not limited to, agents which are capable of inhibiting P-38 (a.k.a., "RK” or "SAPK-2", Lee et al, Nature, 372:739 (1994). P-38 is described as a serine/threonine kinase (see Han et al, Biochimica Biophysica Acta, 1265:224- 227 (1995). Inhibitors of P-38 have been shown to intervene between the extracellular stimulus and the secretion of IL-I and TNF ⁇ from the cell involves blocking signal transduction through inhibition of a kinase which lies on the signal pathway.
- T-cell inhibitors are also suitable, including, for example, ctla-4, CsA, Fk-506, OX40, OX40R-Fc, OX40 antibody, OX40 ligand, OX40 ligand antibody, lck, and ZAP70.
- retinoids including oral retinoids, as well as antagonists of TGF- ⁇ .
- salicylic acid derivatives for example, any of one or more salicylic acid derivatives, prodrug esters or pharmaceutically acceptable salts thereof.
- Such salicylic acid derivatives, prodrug esters and pharmaceutically acceptable salts thereof comprise: acetaminosalol, aloxiprin, aspirin, benorylate, bromosaligenin, calcium acetylsalicylate, choline magnesium trisalicylate diflusinal, etersalate, fendosal, gentisic acid, glycol salicylate, imidazole salicylate, lysine acetylsalicylate, mesalamine, morpholine salicylate, 1-naphthyl salicylate, olsalazine, parsalmide, phenyl acetylsalicylate, phenyl salicylate, salacetamide, salicylamide O-acetic acid, salsalate and sulfasalazin
- Structurally related salicylic acid derivatives having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- suitable agents include, for example propionic acid derivatives, prodrug esters or pharmaceutically acceptable salts thereof.
- the propionic acid derivatives, prodrug esters and pharmaceutically acceptable salts thereof comprise: alminoprofen, benoxaprofen, bucloxic acid, carprofen, dexindoprofen, fenoprofen, flunoxaprofen, fluprofen, flurbiprofen, furcloprofen, ibuprofen, ibuprofen aluminum, ibuproxam, indoprofen, isoprofen, ketoprofen, loxoprofen, miroprofen, naproxen, oxaprozin, piketoprofen, pimeprofen, pirprofen, pranoprofen, protizinic acid, pyridoxipro
- acetic acid derivatives having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- acetic acid derivatives, prodrug esters or pharmaceutically acceptable salts thereof comprise: acemetacin, alclofenac, amfenac, bufexamac, cinmetacin,-clopirac, delmetacin, diclofenac sodium, etodolac, felbinac, fenclofenac, fenclorac, fenclozic acid, fentiazac, furofenac, glucametacin, ibufenac, indomethacin, isofezolac, isoxepac, lonazolac, metiazinic acid, oxametacin, oxpinac, pimetacin, proglumetacin, sulindac, tal
- Structurally related acetic acid derivatives having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- fenamic acid derivatives, prodrug esters or pharmaceutically acceptable salts thereof comprise: enfenamic acid, etofenamate, flufenamic acid, isonixin, meclofenamic acid, meclofenamate sodium, medofenamic acid, mefanamic acid, niflumic acid, talniflumate, terofenamate, tolfenamic acid and ufenamate.
- Structurally related fenamic acid derivatives having similar analgesic and anti- inflammatory properties are also intended to be encompassed by this group.
- carboxylic acid derivatives, prodrug esters and pharmaceutically acceptable salts thereof which can be used comprise: clidanac, diflunisal, flufenisal, inoridine, ketorolac and tinoridine. Structurally related carboxylic acid derivatives having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- butyric acid derivatives, prodrug esters or pharmaceutically acceptable salts thereof comprise: bumadizon, butibufen, fenbufen and xenbucin.
- Oxicams, prodrug esters or pharmaceutically acceptable salts thereof are also suitable.
- Oxicams, prodrug esters and pharmaceutically acceptable salts thereof comprise: droxicam, enolicam, isoxicam, piroxicam, sudoxicam, tenoxicam and 4-hydroxyl-l,2-benzothiazine 1,1 -dioxide 4-(N-phenyl)-carboxamide.
- Structurally related oxicams having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- Pyrazoles, prodrug esters or pharmaceutically acceptable salts thereof are also suitable.
- pyrazoles, prodrug esters and pharmaceutically acceptable salts thereof which may be used comprise: difenamizole and epirizole. Structurally related pyrazoles having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group. Furthermore, pyrazolones, prodrug esters or pharmaceutically acceptable salts thereof are suitable.
- the pyrazolones, prodrug esters and pharmaceutically acceptable salts thereof which may be used comprise: apazone, azapropazone, benzpiperylon, feprazone, mofebutazone, morazone, oxyphenbutazone, phenylbutazone, pipebuzone, propylphenazone, ramifenazone, suxibuzone and thiazolinobutazone.
- Structurally related pyrazalones having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- prodrug esters or pharmaceutically acceptable salts thereof for the treatment of TNF-mediated diseases.
- Corticosteroids, prodrug esters and pharmaceutically acceptable salts thereof include hydrocortisone and compounds which are derived from hydrocortisone, such as 21-acetoxy- pregnenolone, alclomerasone, algestone, amcinonide, beclomethasone, beta ⁇ methasone, betamethasone valerate, budesonide, chloroprednisone, clobetasol, clobetasol propionate, clobetasone, clobetasone butyrate, clocortolone, cloprednol, corticosterone, cortisone, cortivazol, deflazacon, desonide, desoximerasone, dexamethasone, diflorasone, diflucortolone, difluprednate, enoxolone, fluazacort, flucloroni
- Antimicrobials are also suitable for combination use as described herein.
- Suitable antimicrobials include, for example, ampicillin, amoxycillin, aureomicin, bacitracin, ceftazidime, ceftriaxone, cefotaxime, cephachlor, cephalexin, cephradine, ciprofloxacin, clavulanic acid, cloxacillin, dicloxacillan, erythromycin, flucloxacillan, gentamicin, gramicidin, methicillan, neomycin, oxacillan, penicillin and vancomycin.
- Structurally related antimicrobials having similar analgesic and anti-inflammatory properties are also intended to be encompassed by this group.
- Additional suitable compounds include, but are not limited to: BN 50730; tenidap; E 5531; tiapafant PCA 4248; nimesulide; panavir; rolipram; RP 73401; peptide T; MDL 201.449A; (lR,3S)-Cis-l-[9-(2,6-diaminopurinyl)]-3-hydroxy-4- cyclopentene hydrochloride; (lR,3R)-trans-l-[9-(2,6-diamino)purine]-3- acetoxycyclopentane; (1 R,3R)-trans- 1 -[9-adenyl)-3-azidocyclopentane hydrochloride and (lR,3R)-trans-l-[6-hydroxy-purin-9-yl)-3-azidocyclopentane.
- IL-4 can induce an inflammatory effect in some instances, such as in asthma, in which over-expression of IL-4 in the lungs causes epithelial cell hypertrophy and an accumulation of lymphocytes, eosinophils, and neutrophils. This response is representative of the main features of the proinflammatory response induced by other Th2 cytokines. As noted above, therefore, inhibitors of IL-4 are also useful in accordance with the invention. Additionally, it will be appreciated that certain immunosuppressant drugs can also be used in the treatment of arthritis, including, but not limited to, iNOS inhibitors, and 5 -lipoxygenase inhibitors.
- Ginger has been shown to have certain anti-inflammatory properties, and is therefore suitable for use as an anti-inflammatory agent in accordance with the invention, as is chondroitin.
- a specific binding agent to Ang-2 may be administered prior to, concurrent with, and subsequent to treatment with a cancer therapy agent.
- Exemplary cancers include, but are not limited to, breast cancer, colorectal cancer, gastric carcinoma, glioma, head and neck squamous cell carcinoma, hereditary and sporadic papillary renal carcinoma, leukemia, lymphoma, Li-Fraumeni syndrome, malignant pleural mesothelioma, melanoma, multiple myeloma, non-small cell lung carcinoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, small cell lung cancer, synovial sarcoma, thyroid carcinoma, and transitional cell carcinoma of urinary bladder.
- a specific binding agent to Ang-2 may be used alone or with at least one additional therapeutic agents for the treatment of cancer.
- a specific binding agent to Ang-2 is used in conjunction with a therapeutically effective amount of an additional therapeutic agent.
- exemplary therapeutic agents that may be administered with a specific binding agent to Ang-2 include, but are not limited to, a member of the geldanamycin family of anisamycin antibiotics; a Pro-HGF; NK2; a c-Met peptide inhibitor; an antagonist of Grb2 Src homology 2; a Gabl modulator; dominant-negative Src; a von-Hippel-Landau inhibitor, including, but not limited to, wortmannin; P13 kinase inhibitors, other anti-receptor therapies, anti EGFR, a COX-2 inhibitor, CelebrexTM, VioxxTM; a vascular endothelial growth factor (VEGF), a VEGF modulator, a fibroblast growth factor (FGF), an FGF modulator, an epidermal growth factor (EGF); an EGF modulator; a keratinocyte growth factor (
- the present invention is directed to therapies comprising a specific binding agent to Ang-2 and at least one serine protease inhibitor, and methods of treatment using such therapies.
- a therapy comprises a specific binding agent to Ang-2 and a serine protease inhibitor and at least one additional molecule described herein.
- a disturbance of the protease/protease inhibitor balance can lead to protease-mediated tissue destruction, including, but not limited to, tumor invasion of normal tissue leading to metastasis.
- a specific binding agent to Ang-2 may be used with at least one therapeutic agent for inflammation.
- a specific binding agent to Ang-2 may be used with at least one therapeutic agent for an immune disorder.
- exemplary therapeutic agents for inflammation and immune disorders include, but are not limited to cyclooxygenase type 1 (COX-I) and cyclooxygenase type 2 (COX-2) inhibitors small molecule modulators of 38 kDa mitogen-activated protein kinase (p38 -MAPK); small molecule modulators of intracellular molecules involved in inflammation pathways, wherein such intracellular molecules include, but are not limited to, jnk, IKK, NF- ⁇ B, ZAP70, and lck.
- Certain exemplary therapeutic agents for inflammation are described, e.g., in CA. Dinarello and L.L. Moldawer Proinflammatory and Anti- Inflammatory Cytokines in Rheumatoid Arthritis: A Primer for Clinicians Third Edition (2001) Amgen Inc. Thousand Oaks, CA.
- compositions will include more than one different a specific binding agent to Ang-2. In certain embodiments, pharmaceutical compositions will include more than one a specific binding agent to Ang-2 wherein the specific binding agents to Ang-2 bind more than one epitope.
- Immunotherapeutics generally rely on the use of immune effector cells and molecules to target and destroy cancer cells.
- the immune effectors may be, for example an antibody of the present invention that recognizes some marker on the surface of a target cell.
- the antibody alone may serve as an effector of therapy or it may recruit other cells to actually affect cell killing.
- the antibody may also be conjugated to a drug or toxin (chemotherapeutic, radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) and thus may merely serve as a targeting agent.
- mutant forms of Ang-2 may be targeted by immunotherapy either antibodies or antibody conjugates of the invention. It is particularly contemplated that the antibody compositions of the invention may be used in a combined therapy approach in conjunction with Ang-2 targeted therapy.
- Ang-2 expression was examined in normal and pathological tissue using in situ hybridization. Fragments of the human (Genbank Accession Number:
- Ang-2 sequences were amplified by reverse transcriptase-PCR from human or murine fetal lung cDNA, cloned into the pGEM-T plasmid and verified by sequencing. 33p_i a b e i ec ⁇ antisense RNA probes were transcribed from linearized plasmid templates using 33p -UTP and RNA polymerase. Blocks of formaldehyde-fixed, paraffin-embedded tissues were sectioned at 5 ⁇ m and collected on charged slides.
- tissues Prior to in situ hybridization, tissues were permeabilized with 0.2M HCL, followed by digestion with Proteinase K, and acetylation with triethanolamine and acetic anhydride. Sections were hybridized with the radio labeled probe overnight at 55 0 C then subjected to RNase digestion and a high stringency wash in about 0.1X SSC at 55°C. Slides were dipped in Kodak NTB2 emulsion, exposed at 4°C for 2-3 weeks, developed, and counterstained. Sections were examined with dark field and standard illumination to allow simultaneous evaluation of tissue morphology and hybridization signal.
- Ang-2 expression is restricted to the few tissues containing angiogenic vasculature, such as the ovary, placenta, and uterus.
- No Ang-2 expression was detectable in normal adult human heart, brain, kidney, liver, lung, pancreas, spleen, muscle, tonsil, thymus, appendix, lymph node, gall bladder, prostate or testis.
- kidneys displayed prominent Ang-2 expression in the vasa recta. To determine whether this expression was a remnant of embryonic development, this experiment was repeated on kidneys derived from mice ranging in age up to one-year-old using the murine Ang-2 probe and conditions described above.
- Ang-2 expression was observed to decrease during post-natal development, but was still evident in kidneys of one-year-old mice. Ang-2 expression was also detected in virtually all tumor types tested, including, primary human tumors such as colon carcinoma (5 cases), breast carcinoma (10 cases), lung carcinoma (8 cases), glioblastoma (1 case), metastatic human tumors such as breast carcinoma (2 cases), lung carcinoma (2 cases) and ovarian carcinoma (2 cases) which had metastized to brain, and rodent tumor models such as C6 (rat glioma), HT29 (human colon carcinoma), Colo-205 (human colon carcinoma), HCTl 16 (human colon carcinoma), A431 (human epidermoid carcinoma), A673 (human rhabdomyosarcoma), HT 1080 (human fibrosarcoma), PC-3 (human prostate carcinoma), Bl 6F10 (murine melanoma), MethA (murine sarcoma), and Lewis lung carcinoma mets. Additionally, Ang-2 expression was detected in neovessels growing into a Ma
- Full length, His-tagged murine Ang-2 cDNA was obtained by PCR (Clontech Advantage PCR Kit, Cat. # K1905-01) from a murine 15-day embryo cDNA library (Marathon-Ready-cDNA, Cat.# 7459-1, Clonetech, Inc.) using PCR primers for full length human Ang-2.
- the PCR product was ligated into a CMV promoter expression vector, and the resultant plasmid was transfected into HTl 080 human fibrosarcoma cells (obtained from ATCC) using FuGENE ⁇ Transfection Reagent (Roche, Cat. #1814443). Stable clones were isolated by G418 selection. Anti-His tag ELISAs and Western blotting were used to screen for mAng-2-his expressing clones. Recombinant mAng-2 polypeptide was purified from conditioned media
- CM. CM. of these cells.
- the CM. containing mAng-2-His was purified by a two- step chromatography protocol. Briefly, the conditioned media was titrated to pH 8.9 by adding Tris buffer pH 9.5 to about 20 mM final concentration. Additionally, the detergent CHAPS was added to about 5 mM final concentration. The CM. was then applied directly to an anion exchange column Q-sepharose ff (Pharmacia). The column was then washed with about 10 mM Tris pH 8.0 containing about 50 mM NaCl.
- Recombinant mAng-2-His was eluted in a single step using 10 mM Tris pH 8.0 containing about 350 mM NaCl and about 5 mM CHAPS.
- the eluate from the Q-sepharose column was adjusted to about 4 mM imidazole, and applied to an immobilized metal affinity column (Ni-NTA superflow (Qiagen)).
- the bound protein was eluted with PBS containing about 5 mM CHAPS and about 100 mM imidazole.
- the eluate was then concentrated to approximately 1.0 mg/ml, followed by dialysis against PBS.
- the purity of mAng-2-His was greater than 90 percent as measured by SDS-PAGE Coomassie staining.
- Rabbits were immunized with about 0.2 mg mAng-2/injection in an attempt to produce antibodies. Rabbits were injected with about 1 mL Hunter's TiterMax® (Sigma) and mAng-2 at a ratio of 1 : 1. Four weeks later, each rabbit received a repeat injection or booster; two weeks after that, they received their next booster, and at week seven, sera were drawn and evaluated for titer against mAng-2. If the serum titer was high, 50 mL production bleeds were drawn on a weekly basis for six consecutive weeks. However, if serum titer was low, rabbits were given an additional booster, and 50 mL production bleeds were drawn on a weekly basis for six consecutive weeks, beginning at week 9. After six consecutive production bleeds, rabbits were allowed to rest for six weeks. If more sera were required, the rabbits were boosted again one month after the last production bleed.
- EXAMPLE 3 Molecular Assays to Evaluate Ang-2 Antibodies Molecular assays (Affinity ELISA, Neutralization ELISA and BIAcore) were developed to assess direct antibody binding to Ang-2 and related family members, and the effect of antibodies on the Ang-2:Tie2 interaction. These in vitro and cell-based assays are described as follows.
- human Ang-2 R and D Systems, Inc; catalog number 623 -AN; Ang-2 is provided as a mixture of 2 truncated versions
- murine Ang-2 polypeptide prepared as described above
- human Ang-2 was obtained from conditioned media of human 293T cells transfected with full length human Ang-2 DNA and cultured in serum free DMEM containing about 50 micrograms per ml of bovine serum albumin (BSA).
- BSA bovine serum albumin
- Ang-2 was added to each well and the plates were incubated about 2 hours, after which the plates were washed with phosphate buffered saline (PBS) containing about 0.1 percent Tween-20 four times. The wells were then blocked using about 250 microliters per well of about 5 percent BSA in PBS, and the plates were incubated at room temperature for about 2 hours. After incubation, excess blocking solution was discarded, and about 100 microliters of candidate anti-Ang-2 antibody was added to each well in a dilution series starting at a concentration of about 40 nanomolar and then serially diluting 4-fold in PBS containing about 1 percent BSA. The plates were then incubated overnight at room temperature.
- PBS phosphate buffered saline
- Microtiter plates to which human Ang-2 polypeptide was bound were prepared as described for the Affinity ELISA.
- Candidate anti- Ang-2 antibodies were prepared in serial dilutions as described for the Affinity ELISA above in a solution of PBS containing about 1 percent BSA and about 1 nM Tie2 (provided as a Tie2-Fc molecule where the Tie2 portion contains only the soluble extracellular portion of the molecule; R and D Systems, catalog number 313-TI). After about 100 microliters of the antibody/Tie2 solution was added to each well, the plates were incubated overnight at room temperature, and then washed five times in PBS containing about 0.1 percent Tween-20.
- BIAcore ® 2000 (Biacore, Inc., Piscataway, NJ) with PBS and 0.005 percent P20 surfactant (BIAcore, Inc.) as running buffer.
- Recombinant Protein G Repligen, Needham, MA
- Binding assays were carried out by first attaching about 100 Ru of each candidate anti-Ang-2 antibody to the immobilized Protein G, after which various concentrations (0 - 100 nM) of huAng-2 or m Ang-2 were then injected over the bound antibody surface at a flow rate of about 50 ul/min for about 3 minutes.
- Antibody binding kinetics including k a (association rate constant), k d (dissociation rate constant) and Kp (dissociation equilibrium constant) were determined using the BIA evaluation 3.1 computer program (BIAcore, Inc.). Lower dissociation equilibrium constants indicated greater affinity of the antibody for Ang-2.
- Fully human Ang-2 antibodies were generated by panning a Target Quest Phage Display Fab library (Target Quest, Inc.) against a human Ang-2 polypeptide (R and D Systems Inc., catalog 623-AN), according to the following protocol.
- Human Ang-2 was immobilized on the surface of polystyrene magnetic beads by two methods: (1) direct coating of Ang-2 at 50 ug/ml at 4C overnight; and (2) indirect capture of Ang-2 by goat anti-Ang-2 antibody at 50 ug/ml at 4C overnight.
- the bead surface was blocked by 2% milk in PBS (MPBS).
- the human Fab phage library was pre-selected to remove phage clones reactive to uncoated magnetic beads or the goat anti-Ang-2 antibody.
- Ang-2-coated magnetic beads were then incubated with library phage at room temperature for 1.5 hours.
- the surface was washed 6 times with MPBS containing about 0.1 percent Tween 20, followed by washing 6 times with PBS containing about 0.1 percent Tween 20, followed 2 times with PBS.
- the bound phage were eluted first with about 100 ug/ml human Tie2-Fc (R and D Systems, Minneapolis, MN), and then with about 100 mM triethanolamine. Eluted phage were infected into E. coli TGl cells, amplified, and rescued for the next round of screening. Selection pressure was increased in successive rounds by incorporating more stringent washes and reducing the number of input phage.
- the immunotube surface was blocked using about 2 percent milk in PBS (MPBS), before it was incubated with about 2 trillion phage particles (about 50 copies of each unique phage in the library) from the same phage display library referred to above (Target Quest) in about 4ml of 2% MPBS. After the phage incubation step, the surface was washed 20 times with PBS plus about 0.1 percent Tween 20, followed 20 times with PBS. The bound phage were eluted using IuM hAng-2 or IuM human Tie2 (R and D Systems, described above). Eluted phage were infected into E. coli TGl cells (provided with the phage library), amplified, and rescued for the next round of screening.
- Ang-2-binding Fab clones were identified by PCR amplification of all phage to which hAng-2 or Tie2 bound, and these clones were analyzed by restriction digestion.
- the DNA of each clone was sequenced.
- the sequence coding for the variable region of each heavy chain from each phage was amplified with complementary primers.
- the primers were designed to incorporate a HindIII site, Xbal site, Kozak sequence and a signal sequence (translated peptide is MDMRVP AQLLGLLLL WLRGARC; SEQ ID NO: 202) onto the 5' end of the variable region, while a BsmBI site was added onto the 3' end of the PCR product.
- the template phage DNA for clone 544 (SEQ ID NO: 19) was amplified using primers 2248-21 (GTG GTT GAG AGG TGC CAG ATG TCA GGT CCA GCT GGT GCA G; SEQ ID NO: 203) which added the last 7 amino acids of the signal sequence and 2502-31 (ATT ACG TCT CAC AGT TCG TTT GAT CTC CAC; SEQ ID NO: 204) which added the BsmBI site onto the end of the variable region.
- the resulting product was amplified by primers 2148-98 (CCG CTC AGC TCC TGG GGC TCC TGC TAT TGT GGT TGA GAG GTG CCA GAT; SEQ ID NO: 205) which added nine amino acids to the signal peptide (AQLLGLLLL; SEQ ID NO: 206) and 2502-31 , and then 2489-36 (CAG CAG AAG CTT CTA GAC CAC CAT GGA CAT GAG GGT CCC CGC TCA GCT CCT GGG; SEQ ID NO: 207) and 2502-31.
- Primer 2489-36 added, from 5' to 3', the HindIII site, Xbal site, Kozak sequence, and the first 6 amino acids of the signal sequence.
- the PCR products were digested with Xbal and BsmBI, and then cloned into a mammalian expression vector containing the human IgGl constant region. This vector contains an SV40 promoter and DHFR selection.
- Light chains from each phage were either kappa or lambda class.
- complementary primers were designed to add, from 5' to 3', a HindIII site, an Xbal site, Kozak sequence and signal sequence (set forth above). Those chains which had error-free coding regions were cloned as full-length products.
- the light chains from phage clone 536 was amplified as a full length coding region using primers 2627-69 (GTG GTT GAG AGG TGC CAG ATG TGA CAT TGT GAT GAC TCA GTC TCC; SEQ ID NO: 208), which added the last seven amino acids of the signal sequence, and primer 2458-54 (CTT GTC GAC TTA TTA ACA CTC TCC CCT GTT G; SEQ ID NO: 209), which added a Sail site after the stop codon.
- This PCR product was then amplified as previously stated with additional 5' primers, 2148-98 and 2489-36 respectively, paired with primer 2458-54, to finish addition of the signal sequence and cloning sites.
- the full-length light chains were cloned as Xbal-Sall fragments into the mammalian expression vector described above.
- Certain lambda clones had errors in their constant regions when compared to natural human constant region sequence.
- overlap PCR was performed using DNA coding for a perfect lambda constant region and the phage derived variable region. These clones were also cloned as Xbal-Sall fragments as described above.
- kappa variable regions were cloned separately from their constant regions, a BsmBI site was added to the 3' end of the PCR product. After digestion of the PCR product with Xbal and BsmBI, the kappa chain variable region was cloned into an expression vector containing the human kappa constant region.
- the paired light and heavy chain constructs from each converted phage were co-transfected into CHO cells using the Calcium Phosphate Transfection Kit (Invitrogen Corp.) generally according to the manufacturer's suggested protocol. Media was changed 14-16 hours post transfection, and the cells were passaged into tissue culture dishes for selection after about 48 hours per the manufacturer's recommendations. Transfected cells were isolated by HT selection for approximately 3 weeks, at which time transfected CHO cell colonies were trypsinzed and combined into a "pool" of transfected cells.
- Small-scale conditioned media was collected after 48 hours and assayed for antibody production by Western blot analysis using either anti-human Fc antibody, anti-human kappa antibody, or anti-human lambda antibody.
- the selected cell populations were then passaged under selective pressure using standard tissue culture sterile technique until enough cells were obtained to seed four 850 cm 2 roller bottles with 2x10 7 viable cells each, and to prepare frozen stock cell lines using DMSO. After seeding, the cells were maintained in roller bottles with about 10 percent serum containing DMEM medium (Gibco/BRL, Inc) supplemented with glutamine and non-essential amino acids. Cells were maintained for two to three days until a cell confluency of approximately 80% was reached.
- DMEM medium Gibco/BRL, Inc
- the media in the roller bottles was switched to a serum free media mixture (50 percent DMEM, 50 percent F 12, Gibco) supplemented with glutamine and non-essential amino acids.
- a serum free media mixture 50 percent DMEM, 50 percent F 12, Gibco
- Conditioned media was harvested after seven days, with fresh serum- free medium being added for one or two additional harvests.
- Antibodies were purified by Protein G affinity chromatography directly from conditioned medium, using standard procedures. Elution from the Protein G column was accomplished using low pH (about pH 3) buffer, after which the eluted antibody protein was neutralized using IM Tris, pH 8.5, and then concentrated using 10 kD molecular weight cutoff centrifugal concentrators. The concentrated antibody stock was then buffer exchanged into PBS.
- lambda light chain constant regions appear to be chimers of more than one germline lambda constant region gene.
- the closest lambda constant region germline gene is indicated along with the amino acids differing from that germline gene, numbered using the Kabat system.
- the following four tables set forth the sequences and SEQ ID NOs. of the heavy and light (kappa and lambda) chains of the 31 anti-Ang-2 antibodies converted from phage to full length IgGl antibodies.
- the complementarity- determining regions (CDRs) of the monoclonal antibodies were predicted using the VBASE database which uses the technique described by Kabat et al in:
- CDRs Complementarity-Determining Regions (CDRs) of Heavy Chains (HC) and Light
- the clone 536 analyzed above comprised a mixture of two antibody variants, which are shown in Table 4 as SEQ ID NO: 12 (536 kappa THW) and SEQ ID NO: 210 (536 kappa LQT). These two 536 variants were separated and analyzed separately for potency using ELISA and HTRP assays.
- ELISA assay ninety-six-well microtiter plates were coated with recombinant angiopoietins in 293 T cell conditioned media (DMEM/50 ug/ml BSA) at 37°C for 1 hour.
- the conditioned media were used at angiopoietin concentrations that conferred 80% of maximally-achievable binding to 1 nM hTie2-Fc (R&D Systems, catalog # 313-TI). Plates were washed with PBS/0.1% Tween-20 and then blocked for 2 hours at room temperature with PBS/5% BSA. Angiopoietin-neutralizing agents titrated from 100 nM to 0.4 pM in a solution of PBS/1% BS A/1 nM Tie2 were added to the angiopoietin-coated plates, which were incubated overnight at room temperature and then washed with PBS/0.1 percent Tween-20.
- Mouse-derived anti-Tie2 antibody (BD Pharmingen Inc., catalog # 557039) was added to each plate at a final concentration of 1 ug/ml (1 hour incubation at room temperature), after which plates were washed in PBS/0.1% Tween-20.
- Goat anti-mouse-IgG-HRP (Pierce, catalog # 31432) was added at a dilution of 1 :10,000 in PBS/1% BSA (1 hour incubated at room temperature), after which plates were washed several times with PBS/0.1 % Tween-20.
- TMB substrate (Sigma, catalog # T8665) was added, O.D. 370 nm absorbance was measured, and degree of angiopoietin:Tie2 neutralization was determined by comparison against a Tie2 standard curve.
- HTRF assays For HTRF assays, a ninety-six-well microtiter "mix plate" was prepared by adding 50 ul of HTRF buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween 20, 0.1% BSA) containing 0.8 nM Europium-conjugated streptavidin (PERKIN ELMER LIFE SCIENCES INC., catalog # AD0062) and 4.0 nM biotinylated human angiopoietin 1 (R & D Systems) or angiopoietin 2 (Amgen Inc.) to each well.
- HTRF buffer 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween 20, 0.1% BSA
- 0.8 nM Europium-conjugated streptavidin PERKIN ELMER LIFE SCIENCES INC., catalog # AD0062
- angiopoietin-neutralizing agents were titrated from 400 nM to 20 pM in HTRF buffer, and 50 ul of each serially- diluted angiopoietin-neutralizing agent were then transferred and mixed with the "mix plate” containing the streptavidin-Europium/angiopoietin. The plate was then incubated on a shaker at room temperature for one hour. Next, 20 ul from each well of the "mix plate” were transferred to an "assay plate" containing 20 ul of 10 nM human allophycocyanin-conjugated human Tie2-Fc in HTRF buffer in each of the ninety-six- wells.
- the final “assay plate” was incubated at room temperature with shaking for two hours.
- the final concentrations of the “assay plate” were: 1.0 nM angiopoietin, 5.0 nM human Tie2-Fc, and 100 nM to 5.0 pM for the serially-diluted angiopoietin-neutralizing agents.
- Assay plates were analyzed using a Rubystar plate reader (BMG Labtechnologies, Offenberg, Germany).
- Degree of angiopoietin:Tie2 neutralization was determined by calculating the percentage inhibition of each angiopoietin-neutralizing agent dilution using a "no-angiopoietin-neutralizing agent" control (to represent zero inhibition) and a "no-angiopoietin” control (representing complete inhibition).
- IC50 values were then calculated by analyzing percentage inhibition using the GRAFIT 5.0 program (Erithacus Software Ltd.). All results were expressed as IC50 curves calculated from samples that were tested in duplicate using the formula below. IC50 results fit inhibition data to a 2 parameter equation, where the lower data limit was 0, (i.e. the data were background corrected, and the upper data limit was 100, (i.e. the data were range corrected).
- mice The pharmacokinetics of Protein-G purified rabbit anti-Ang-2 polyclonal antibodies were examined in mice. Twenty-four mice were treated with polyclonal anti-Ang-2 rabbit antibody (1 mg per mouse). Four treated animals were sacrificed at each of the following time points post-injection of antibody: 1 hour, 6 hours, 1 day, 3 days, 7 days, and 14 days.
- mice (10 animals/group) bearing A431 tumor xenografts were given 10 doses (about 10 mg IgG per mouse per dose) intraperitoneally of Protein G purified anti-niAng-2 polyclonal antibody on days 1, 5, 6, 7, 8, 12, 13, 14, 15, and 18 after xenografting. Tumor size was measured on days 7, 12, 15, 19, and 21. Body weight was measured on days 0, 7, 15, and 21, and was unaffected by treatment.
- results indicated that the anti-Ang-2 polyclonal antibody inhibited the A431 tumor xenograft growth by about 50 percent with p 0.008 versus controls of non-immune purified polyclonal antiserum (10 mg IgG per mouse per dose) and vehicle (PBS) by repeated measures ANOVA.
- mice (10 animals/group) bearing A431 tumor xenografts were treated intraperitoneal ⁇ with either anti-Ang-2 antibody clone 533, 537, or 544, or with negative controls of PBS or human IgGl -kappa.
- Dosing was about 420 ug protein per mouse for the first dose, about 140 ug protein per mouse for each of the next three doses, and about 55 ug protein per mouse for each of the next four doses, for a total of 8 doses per mouse.
- Tumor volumes and body weights were recorded twice weekly.
- animals were sacrificed and their serum was collected for measuring antibody levels by ELISA. Tumors and a panel of normal tissues were collected from all groups.
- the binding epitope of anti-Ang-2 antibodies and peptibodies was determined by their ability to bind the three versions of human hAng-2 by ELISA according to the following protocol: a high-binding 96- well assay plate was coated with 100 ⁇ l of conditioned media per well, and incubated at 37 0 C for 1 hour. Conditioned media was aspirated, and the plate was blocked with 200 ⁇ l per well of 5% BSA in PBS at room temperature for 1 hour. The blocking solution was then aspirated. 100 ⁇ l per well of antibody, peptibody, or Tie2-Fc was added at l ⁇ g/ml in 1% BSA in PBS, and incubated at room temperature for 1 hour.
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MX2007004247A MX2007004247A (en) | 2004-10-19 | 2005-10-19 | Angiopoietin-2 specific binding agents. |
EA200700876A EA011866B1 (en) | 2004-10-19 | 2005-10-19 | Angiopoetin-2 specific binding agents |
CN200580043569.5A CN101495513B (en) | 2004-10-19 | 2005-10-19 | Angiopoietin-2 specific binding agents |
CA2583910A CA2583910C (en) | 2004-10-19 | 2005-10-19 | Angiopoietin-2 specific binding agents |
AU2005295260A AU2005295260B2 (en) | 2004-10-19 | 2005-10-19 | Angiopoietin-2 specific binding agents |
JP2007538066A JP2008520188A (en) | 2004-10-19 | 2005-10-19 | Angiopoietin-2 specific binding substance |
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BRPI0518209-3A BRPI0518209A (en) | 2004-10-19 | 2005-10-19 | angiopoietin-2-specific binding agents |
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WO2011117329A1 (en) * | 2010-03-26 | 2011-09-29 | F. Hoffmann-La Roche Ag | Bispecific, bivalent anti-vegf/anti-ang-2 antibodies |
RU2466140C2 (en) * | 2007-02-15 | 2012-11-10 | Астразенека Аб | ISOLATED ANTIBODY OR ITS ANTIGEN-BINDING FRAGMENT SPECIFIC FOR HUMAN IMMUNOGLOBULIN E (VERSIONS), NUCLEIC ACID MOLECULE CODING IT, HOST CELL, METHOD FOR PREPARING ANTIBODY OR ITS ANTIGEN-BINDONG FRAGMENT, PHARMACEUTICAL COMPOSITION, USE THEREOF FOR TREATING IgE-ASSOCIATED DISEASES |
WO2012162561A2 (en) | 2011-05-24 | 2012-11-29 | Zyngenia, Inc. | Multivalent and monovalent multispecific complexes and their uses |
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Also Published As
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AU2005295260B2 (en) | 2012-04-12 |
US20090191212A1 (en) | 2009-07-30 |
CA2583910A1 (en) | 2006-04-27 |
AU2005295260A1 (en) | 2006-04-27 |
US7658924B2 (en) | 2010-02-09 |
US20060018909A1 (en) | 2006-01-26 |
US20110311546A1 (en) | 2011-12-22 |
US20130142799A1 (en) | 2013-06-06 |
JP2014040428A (en) | 2014-03-06 |
CA2583910C (en) | 2012-05-01 |
EP1805219A1 (en) | 2007-07-11 |
KR20070085408A (en) | 2007-08-27 |
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