WO2006043553A1

WO2006043553A1 - Method for producing modified gum arabic and use thereof

Info

Publication number: WO2006043553A1
Application number: PCT/JP2005/019123
Authority: WO
Inventors: Tsuyoshi Katayama
Original assignee: San-Ei Gen F.F.I., Inc.
Priority date: 2004-10-19
Filing date: 2005-10-18
Publication date: 2006-04-27
Also published as: JPWO2006043553A1

Abstract

Disclosed is a method for efficiently enhancing emulsification properties of a gum arabic. Also disclosed is a method for producing a gum arabic so modified as to have high emulsification properties. Such a gum arabic is obtained by irradiating an aqueous gum arabic solution with ionizing radiation.

Description

Specification

Process for producing modified gum arabic and its use

Technical field

[0001] The present invention relates to a method for producing modified gum arabic. More particularly, the present invention relates to a novel process for producing water-soluble modified gum arabic having high dairy stability. The present invention also relates to a water-soluble modified gum arabic prepared by a powerful method and its use.

Background art

[0002] Gum arabic is a natural resin prepared by drying rubber-like exudates obtained from stems and branches of plants belonging to the genus Acacia of leguminous family (Acacia: especially Acacia Senegal and Acacia se). is there. Gum arabic can be dissolved in water to a relatively high concentration, and its aqueous solution has emulsifying properties, emulsion stability, thickening properties, protective colloid properties, and film-forming properties. For this reason, gum arabic has been widely used as an emulsifier, an emulsion stabilizer, a thickener, a binder, a film agent, and the like (see, for example, Non-Patent Document 1). Gum arabic is used in a wide variety of fields. For example, it has long been used as an emulsifier, an emulsion stabilizer, or a thickener in the food field. Therefore, it is a material whose food safety has been confirmed empirically.

[0003] However, because gum arabic is collected from various countries scattered in the Saharan region of Africa, natural gum arabia gum is collected based on differences in soil and climate in each production area. There are very large variations in molecular weight and molecular weight distribution. This variation also occurs due to differences in age and harvest time of the gums from which gum arabic is collected. Therefore, when preparing an emulsifier, an emulsion stabilizer, or a thickener using natural gum arabic, a product of a certain quality cannot be obtained due to the lack of natural gum arabic as a raw material. There is a problem with this (for example, see Non-Patent Document 2).

[0004] In addition, the price of gum arabic rises sharply during the poor season because the production amount of gum arabic fluctuates greatly due to natural factors such as the climate and the conditions of the production area. For this reason, it is necessary to perform a function so as to exhibit functions such as emulsifying property, emulsifying stability, thickening property, and binding property in as little amount as possible. There is a need to modify beer gum.

[0005] For example, as a method for reducing the imbalance in emulsifiability due to variations in natural gum arabic and increasing emulsifying power, (1) arabic acid can also be used to remove arabic acid from metal ions. (2) A method of denaturing gum arabic with a loss on drying of 50% by weight or less at 60 to 140 ° C for 30 minutes or longer. (See, for example, Patent Document 2); (3) A method of modifying gum arabic by heating to 40 ° C or higher in an atmosphere of relative humidity 30 to LOO% (see, for example, Patent Document 3); And (4) A method of modifying gum arabic by heating under the condition of loss on drying of 3% or less (for example, see Patent Document 4).

However, with the method (1), gum arabic having high emulsifiability as expected cannot be obtained, and it is not sufficient as a method for modifying gum arabic. The methods (2) to (4) can certainly reduce the quality variation and improve the emulsifying power, but in order to have a sufficient emulsifying effect, heating at a high temperature for a long time is required. Therefore, depending on the processing conditions, coloring and burnt odor (flavoring) may occur, and depending on the application used, the flavor and appearance may be impaired.

[0007] By the way, radiation irradiation technology is widely used for the purpose of modifying polymer substances such as polyvinyl alcohol (PVA), polyethylene oxide (PEO), and polyvinylpyrrolidone (PVP). Carboxymethylcellulose (CMC) and its derivatives, CM starch, CM chitosan, and cellulose derivatives, methylcellulose, hydroxypropylcellulose, are charged with ionizing radiation when added to water and kneaded into a paste. It is known that when irradiated, polymerization occurs and gel strength is improved, or it becomes a hydrated gel and water absorption is improved (for example, Patent Documents 5 and 6).

[0008] However, with regard to polysaccharides other than the above-described polymeric substances, generally, when irradiated with radiation, the main chain of the saccharide is cleaved, the decomposition reaction proceeds, the molecular weight decreases, and emulsification occurs. It is known that physical properties such as property, viscosity, and binding properties are reduced! /, (See Non-Patent Document 3).

[0009] Gum arabic is no different from this report, and when irradiated with gum arabic balls, crushed products, and spray-dried powders, unlike the polymer materials described above, the polymerization reaction is not observed. In It is known that the viscosity slightly decreases (see Non-Patent Document 4). Thus, there has been no prediction that the function can be improved by irradiating gum arabic with radiation.

Non-patent document 1: iNDUSTRIAL GUMS-Polysaccharides and Their Derivatives- ", SE COND EDITION, ACADEMIC PRESS, New York and London, 1973, pp.197-263 Non-patent document 2: Williams, PA and Phillips, GO, (2000) in Handbook of Hydrocolloids, Editors Williams, PA and Phillips, GO pp.155—168, Woodhead, London and New York

Non-Patent Document 3: "The effects of radiation on carbohydrates" Phillips, CO., Chapter 26 P.1217— 1297, The Carbohydrates Second edition.

Non-Patent Document 4: “The effect of sterilizing doses of y-irradiation on the molecular wei ght and emulsification properties of gum ArabicJ Blake, et al, Food Hydrocolloids 1

988, Vol2 No.5 P.407-415

Patent Document 1: Japanese Patent Laid-Open No. 02-049001

Patent Document 2: JP 2000-166489 A

Patent Document 3: Japanese Patent Application No. 2002-156166

Patent Document 4: Japanese Patent Application No. 2003-318958

Patent Document 5: Japanese Unexamined Patent Application Publication No. 2004-45543

Patent Document 6: Japanese Unexamined Patent Publication No. 2003-160602

Disclosure of the invention

Problems to be solved by the invention

[0010] An object of the present invention is to provide a novel method for producing gum arabic that is modified to have high emulsifying power. Furthermore, an object of the present invention is to provide gum arabic excellent in emulsifying power obtained in this way and use of the gum arabic as an emulsifier.

Means for solving the problem

[0011] The inventors of the present invention have made extensive studies based on the above-mentioned problems. As a result, the gum arabic in the aqueous solution is irradiated with ionizing radiation such as γ rays and electron beams. It has been found that the molecular weight of the gum increases and the emulsifying power of gum arabic improves.

[0012] The present invention has been developed on the basis of strong knowledge and includes the following aspects:

Item 1. A method for producing modified gum arabic, comprising a step of irradiating an aqueous gum arabic solution with ionizing radiation.

Section 2. production method of the modified gum arabic of claim 1, wherein the concentration of gum arabic gum arabic aqueous material is from 15 to 60 weight ^_0/0.

Item 3. The process for producing a modified gum arabic according to Item 1, wherein the ionizing radiation is gamma rays.

Item 4. The method for producing modified gum arabic according to Item 1, wherein the ionizing radiation is an electron beam having an acceleration voltage of 0.2 to: LOMeV.

Item 5. The method for producing modified gum arabic according to Item 1, wherein the absorbed dose of ionizing radiation is 1 to 50 kGy.

Item 6. 3 Arabic gum concentration 15 to 50 weight ^_0/0 to gum arabic water was irradiating ionizing radiation absorbed dose 10~50kGy or arabic gum concentration 50-60% by weight of gum arabic water of Item 2. The method for producing modified gum arabic according to Item 1, comprising a step of irradiating ionizing radiation with an absorbed dose of 30 kGy.

Item 7. The method for producing modified gum arabic according to Item 1, wherein the method comprises a drying step after the step of irradiating the gum arabic aqueous solution with ionizing radiation.

Item 8. The process for producing a modified gum arabic according to Item 7, wherein the drying step is spray drying or drum drying.

[0013] Item 9. A modified Arabicum obtained by the production method according to any one of Items 1 to 7.

Item 10. An emulsifier comprising the modified gum arabic described in Item 9 as an active ingredient.

[0014] Item 11. A method for preparing an emulsified composition using the modified gum arabic described in Item 9 as an emulsifier.

Item 12. Emulsion composition strength The method for preparing an emulsion composition according to Item 11, which is oZw type emulsion or wZoZw type emulsion. Item 13. Using modified gum arabic as described in Item 9 as an emulsifier, essential oil, oily fragrance, oily pigment, oil-soluble vitamin, polyunsaturated fatty acid, animal and vegetable oil, sucrose acetate isobutyrate ester, and medium Item 12. The method for preparing an emulsified composition according to Item 11, comprising a step of dispersing and stabilizing at least one selected hydrophobic substance in a hydrophilic solvent.

[0015] An emulsified composition prepared using the modified gum arabic described in Item 14. as an emulsifier.

Item 15. The emulsified composition according to Item 14, obtained by the preparation method according to Item 11.

Item 16. The emulsified composition according to Item 14, which is a food or drink.

The invention's effect

[0016] According to the method of the present invention, a modified Arabica gum excellent in emulsifying power, particularly emulsification stability, is obtained by irradiating a natural (unmodified) gum arabic aqueous solution used as a raw material with ionizing radiation. Can be obtained. In particular, the present invention adopts the above-mentioned treatment method, which is a problem in the conventionally known reforming method by heating, and efficiently prevents the agglomeration due to coloring or adhesion during the treatment. This is a useful method by which gum arabic having good emulsifying power can be obtained.

[0017] The modified gum arabic of the present invention thus prepared can be suitably used for emulsification of various hydrophobic substances such as essential oils, oil-based pigments, oil-based fragrances, and oil-soluble vitamins. The emulsion (emulsion composition) prepared using the modified gum arabic of the present invention has a uniform particle size distribution compared to the emulsion prepared using natural gum arabic commonly used, and It is extremely stable because the emulsion particles are agglomerated or coalesced due to abuse such as heating, long-term storage, or aging, and the particles are deteriorated by coalescence.

Brief Description of Drawings

[0018] [Fig. 1] Changes in molecular weight of sample 1 (gum arabic powder) and samples 2 to 5 (20, 35, 50, and 60% by weight aqueous gum arabic gum) when irradiated with an electron beam FIG.

[Figure 2] Diagram showing changes in viscosity when sample 1 (gum arabic powder) and samples 2 to 5 (20, 35, 50 and 60% by weight aqueous gum arabic gum) were irradiated with an electron beam It is.

[3] Sample 1 (gum arabic powder), Arabi sample 2-5 (20, 35, 50 and 60 weight ^_0/0 It is the figure which showed the change of the coloring degree at the time of performing an electron beam irradiation with respect to an agam aqueous solution. BEST MODE FOR CARRYING OUT THE INVENTION

[1] Method for producing modified gum arabic

The present invention is a method for modifying gum arabic to have high emulsifying power, in other words, a method for producing gum arabic obtained by modifying gum arabic to have high emulsifying power. The method of the present invention can be carried out by irradiating an aqueous gum arabic solution with ionizing radiation. The “emulsifying power” as used in the present invention includes both the emulsifying power during the emulsification treatment and its stability over time. In the present specification, “emulsification” means the former characteristic, and “emulsification stability” means “the latter characteristic”.

[0020] In the method of the present invention, gum arabic used as a modification target (starting material) is Acacia Senegal or Acacia seval belonging to the genus Acacia which is a leguminous plant. It is a natural exudate obtained from the trunk and branch strength of these genus plants. Alternatively, it may be subjected to further purification treatment, desalting treatment, or processing such as pulverization or dry spraying.

In the present invention, these raw materials are simply referred to as “gum arabic” t, or “natural gum arabic” or “unmodified gum arabic” in the sense of being distinguished from the modified gum arabic of the present invention.

[0021] Natural gum arabic (A. senegal species) is usually found in North Africa and Ethiopian powers in Senegal and countries in West Africa (Ethiopia, Sudan, Seneganore, Nigeria, -Gier, Ghana), Kenya, Uganda and other East African countries In the Saharan region of Africa, the basin region of the Nile tributary, but in the present invention, it may be derived from any production region without questioning its origin.

[0022] Normally, natural gum arabic can be obtained in the form of a lump, ball, coarsely pulverized product, granule, granule, or powder (including spray-dried powder). In the present invention, regardless of these shapes, V and misalignment can be used as the gum arabic to be modified.

[0023] Aqueous gum arabic solution to be irradiated with ionizing radiation is prepared by dissolving these various forms of natural Arabica gum (unmodified gum arabic) in water. Used here Water to be used is not particularly limited, and may be tap water, distilled water, or ion exchange water. Ion exchange water is preferable.

The concentration of gum arabic in the aqueous gum arabic solution varies depending on the type of gum arabic used and the amount of the gum arabic, and a force in the range of usually 15 to 60% by weight can also be selected. 30-55 is properly preferred weight ^_0/0, more preferably 35 to 50 wt%. If the concentration of gum arabic in the gum arabic aqueous solution is lower than 15% by weight, the modification tends not to proceed sufficiently even when irradiated with ionizing radiation, making it difficult to improve efficiently. On the other hand, when the gum arabia gum concentration exceeds 60% by weight, it becomes difficult to evenly dissolve gum arabic in water evenly. Tends to be lost.

[0025] It should be noted that the gum arabic aqueous solution is prepared by dissolving gum arabic in water that has been heated as necessary, and then removing insoluble matter and mixed waste by centrifugation or filter press filtration. It is preferred (solid-liquid separation).

[0026] Irradiation with ionizing radiation can be performed in a state where the aqueous gum arabic solution thus prepared is placed in an arbitrary container depending on the type of ionizing radiation to be irradiated. However, if the gum arabic aqueous solution has a low fluidity, such as a paste, it is possible to irradiate it with ionizing radiation immediately without placing it in a container.

[0027] The type of ionizing radiation is not particularly limited, and any of ionizing radiation (y-ray, electron beam, X-ray) can be used. Gamma rays or electron beams are preferred.

[0028] Since γ-rays have a larger transmission power than electron beams, they are suitable for irradiating a thick sample. Moreover, as long as γ-rays are transmitted, there is an advantage that the type of container used for storing the sample is not limited. For example, with γ-rays, it is possible to irradiate a large amount of sample in a large drum can stainless steel container, so that a large amount of Arabic aqueous solution can be irradiated (modified) at once. it can.

[0029] The γ-ray irradiation includes irradiation using cesium 137 as a radioisotope and irradiation using conoret 60, and γ-ray irradiation using cobalt 60 is preferable. In the case of cobalt 60, when used in a sealed capsule made of stainless steel, it can be handled safely and easily without problems such as environmental pollution. Compared to the default 60, it has the disadvantages that it is not easy to handle safely and the dose per unit time, that is, the dose rate is low and the irradiation efficiency is poor!

[0030] On the other hand, electron beams do not use radioisotopes such as cobalt 60 and cesium 137 like γ-rays, so shielding devices may be relatively simple, which is preferable in terms of safety and work. A source of radiation. In addition, electron beam irradiation is said to have a high treatment capacity due to a high dose per unit time, that is, a dose rate.

[0031] Although the transmission power of electron beams varies depending on the acceleration voltage, the transmission power is generally smaller than that of gamma rays. For this reason, it is preferable to adjust the irradiation sample (gum arabic gum aqueous solution) according to the transmission power (electron beam energy) of the electron beam used. For example, when using a high energy electron beam of about lOMeV, the thickness of the irradiated sample should be several mn! Even when the thickness is adjusted to be relatively thick, such as about 3 cm, uniform irradiation is possible. On the other hand, when using a low-energy electron beam of IMeV or less, it is preferable to adjust the sample thickness to 3 mm or less in order to irradiate uniformly.

[0032] As described above, the electron beam irradiation is performed with a sample to be irradiated having a small thickness, or a sample whose thickness can be adjusted (for example, powder, fluid, liquid, thinnable solid). If so, it is a more preferable irradiation method from the viewpoint of economical and industrial production because it has a large processing capacity and can be processed in large quantities as described above.

[0033] It is preferable that the electron beam irradiation is performed using an electron accelerator. The acceleration voltage is not particularly limited, but is usually about 0.2 to about LOMeV, preferably about 0.3 to 8 MeV.

[0034] When an electron beam is used as the irradiating ionizing radiation, a sample is usually filled in a polyethylene bag and the thickness thereof is adjusted according to the energy of the electron beam. Specifically, the irradiation sample (gum arabic gum aqueous solution) is filled in a polyethylene bag, and spreads thinly and irradiates it as uniformly as possible. In particular, when using a low energy electron beam of IMeV or less, it is preferable to irradiate the sample in a thin sheet with a sample placed in a polyethylene bag that is as thin as possible. When an electron beam is used in this way, the thickness of the irradiated sample is limited, so in order to efficiently process a large number of samples, the irradiated sample is spread thinly and moved on the conveyor. Irradiation is preferred. In addition to the single-sided force, a method of irradiating the double-sided force is also preferably used.

[0035] It should be noted that these irradiation conditions are selected and used according to various factors such as the fluidity and specific gravity of the gum arabic aqueous solution to be irradiated, or the packaging material (container) that accommodates it. Togashi.

[0036] When a gum arabic solution having fluidity of 15 to 50% by weight of gum arabic is used as an irradiation sample, there is no particular limitation, but from the viewpoint of production efficiency of modified gum arabic, cobalt 60 is used. It is preferable to use ionizing radiation with relatively high transmission power, such as gamma rays and high-energy electron beams of about lOMeV. As a suitable irradiation method, when γ-rays are irradiated using cobalt 60, a method of irradiating a gum arabic gum aqueous solution in a sealable drum can, an Atron can, or a stainless steel container can be exemplified. Also, when irradiating high-energy energy, the gum arabic aqueous solution is sealed in an aluminum or polyethylene container and the thickness is several mn! The method of adjusting the irradiation to about 3 cm or less can be mentioned.

[0037] On the other hand, in the case of using an aqueous gum arabic gum solution (paste) with low fluidity and a gum arabic concentration of 50 to 60% by weight as the irradiated sample, the same as above from the viewpoint of the production efficiency of the modified gum arabic It is preferable to use ionizing radiation with relatively high transmission power, such as gamma rays using cobalt 60 and high-energy electron beams of about lOMeV. At that time, when electron beam irradiation is performed, a polyethylene sheet is laid on the conveyor, and an appropriate thickness (preferably about several mn! To 3 cm or less) is used on the top of the sheet. The paste-like gum arabic aqueous solution adjusted to 1 can be spread and irradiated. This makes it possible to irradiate the gum arabic aqueous solution with an electron beam continuously and in large quantities. It is also possible to put a paste-like gum arabic aqueous solution in a polyethylene bag and adjust the thickness as described above for irradiation.

[0038] The absorbed dose of ionizing radiation applied to the gum arabic aqueous solution is not particularly limited as long as the effect of the present invention is obtained. Although a suitable absorbed dose varies depending on the concentration of gum arabic in the gum arabic aqueous solution, a range power of usually 1 to 50 kGy can be appropriately selected and used. For example, in the case of gum arabic aqueous solution with a gum arabic concentration of 15-50% by weight, ionizing radiation The polymerization rate due to irradiation tends to be slightly slow. For this reason, the absorbed dose is preferably in the range of 10-50 kGy, more preferably in the range of 20-40 kGy. On the other hand, in the case of gum arabic aqueous solution having a gum arabic concentration of 50-60% by weight, the polymerization rate by ionizing radiation tends to be high. For this reason, the preferred absorbed dose range is 3-30 kGy, more preferably 10-25 kGy. The absorbed dose of ionizing radiation can be measured according to a standard method. Specifically, for example, a measurement method using a CTA dosimeter (for example, FTR-125, Fuji Photo Film Co. Ltd, etc.) calibrated with a Fricke dosimeter can be exemplified.

[0039] The gum arabic aqueous solution irradiated with ionizing radiation can be dried as it is or appropriately diluted with water and then dried by a conventional method such as spray drying, drum drying, freeze drying, etc. It can also be provided as quality gum arabic. In addition to the drying method described above, a drying method using a drying apparatus such as a vacuum dryer or a vacuum dryer can also be used as an industrial drying method. In these devices, the pressure in the container containing the gum arabic aqueous solution can be lowered by a water pump or a vacuum pump, and the content (gum arabic gum aqueous solution) can be uniformly mixed with a screw or the like. However, since the apparatus can be equipped with an apparatus that can heat the contents by introducing steam into the jacket outside the container, operations such as drying (moisture removal), mixing, and heating are performed once. Can be done. Further, according to the apparatus, after the heating is completed, the contents can be rapidly cooled by mixing the contents while passing cooling water through the jacket outside the container. Examples of these specific devices are: Ribocon (conical ribbon vacuum dryer (RM-VD type): manufactured by Okawara Seisakusho Co., Ltd.), vacuum type Nautamixa NXV type (manufactured by Hosokawa Micron Co., Ltd.), planetary motion type cone Examples include a type mixing dryer SV mixer (manufactured by Shinko Pantech Co., Ltd.). The form of the modified gum arabic prepared by the drying treatment is not particularly limited, and examples thereof include powder, granules and granules.

[0040] According to the production method of the present invention described above, an Arabidopsis modified so as to exhibit high emulsifying power, particularly high emulsification stability, compared with natural (unmodified) gum arabic used as a raw material. Gum can be manufactured and acquired. Among the modified gum arabic thus obtained, emulsifying power ( Particularly preferred from the viewpoint of emulsion stability) are those having a weight average molecular weight of 1 million or more.

[0041] Further, according to the method of the present invention, it is difficult to apply to other products as an additive or as an additive such as coloring, flavoring or caking, which is a problem in the conventional heating method. With reduced inconvenience, gum arabic with improved emulsifying power can be produced and obtained. Therefore, the present invention can also be referred to as a method of modifying gum arabic so as to increase emulsifying power, particularly emulsification stability, in a state where coloring is reduced.

[0042] Therefore, the modified gum arabic obtained by the production method of the present invention is suitable as an emulsifier for foods, cosmetics, pharmaceuticals or quasi-drugs, particularly products having a great influence on the quality of color and odor. Can be used for In addition to the ionizing radiation irradiation process or ionizing radiation irradiation process and the drying process described above, the method for producing the modified gum arabic of the present invention further includes various products (food, aroma, etc.) using the modified gum arabic as an emulsifier. It may be provided with processing steps necessary for application to cosmetics, pharmaceuticals or quasi-drugs! In addition, a treatment step for preparing a suitable state (composition or form) as an emulsifier may be provided.

[0043] (2) Preparation method of emulsifier and emulsified composition

The modified gum arabic prepared by the above method can be clearly distinguished from unmodified gum arabic (natural gum arabic) used as a raw material with high emulsifying power. In general, the emulsifying power of an emulsifier is evaluated to be superior as the average particle size of the prepared emulsion is smaller and the particle size is more stably maintained over time (“Emulsification with Arabic gum”). OZW Emulsion by turbidity ratio method ", Pharmaceutical Journal, 112 (12), 90 6-913, (1992)). Here, regarding the preparation method of emulsion (emulsion composition) used for the evaluation of the emulsifying power of the modified gum arabic, the measurement method of the average particle diameter, and the evaluation method of stability over time (emulsification stability) The method described in the experimental examples described later can be followed.

[0044] The modified gum arabic of the present invention is suitably used as an emulsifier, particularly as an emulsifier for products that can be taken orally, particularly in the field of food, pharmaceuticals, quasi drugs, or cosmetics. be able to. Specifically, beverages, powdered beverages, desserts, chewing gum It can be suitably used as an emulsifier for dairy products such as foodstuffs, tablet confectionery, snack confectionery, processed fishery products, processed livestock products, and retort foods, emulsified oily fragrances, and emulsified oily pigments. The modified gum arabic can be used as an emulsifier in the state of an aqueous solution as it is after irradiation with ionizing radiation or in the solid state after drying (for example, in the form of particles or powder). In addition, other carriers and additives can be blended to prepare an emulsifier. In this case, the carrier or additive used depends on the type of product using the emulsifier (for example, food, medicine, quasi-drug, or cosmetic) and its use.

These can be appropriately selected and employed in accordance with conventional methods. For example, it can be used by mixing with saccharides such as dextrin, maltose and lactose and polyhydric alcohols such as glycerin and propylene glycol. When preparing an emulsifier by blending carriers and additives, the proportion of the modified gum arabic contained in the emulsifier is not limited, but at least the lower limit is

It is desirable that the content be 5% by weight or more, preferably 10% by weight or more. The upper limit is not particularly limited as long as it is less than 100% by weight! ,.

[0045] The present invention also provides a method for preparing an emulsion (emulsified composition) using the modified gum arabic as an emulsifier. The emulsion can be prepared by dispersing and stabilizing a hydrophobic substance (dispersoid) in a hydrophilic solvent (dispersion medium) using the modified gum arabic as an emulsifier. Examples of emulsion (emulsified composition) include oil-in-water (oZw) type and wZoZw type emulsion.

[0046] The hydrophobic substance to be emulsified here is not particularly limited as long as it is ordinarily provided in emulsion form, but is preferably in the food, pharmaceutical, quasi-drug or cosmetic field. And edible hydrophobic substances that can be used orally.

[0047] Specifically, various essential oils obtained from basic plants such as citrus plants such as orange, lime, lemon and grapefruit, and basic plant powers such as pepper, cinnamon and ginger. Oleoresin and jasmine obtained by oleoresin method Oils, such as absolute, obtained from the basic plant such as rose and rose, etc., other synthetic fragrance compounds, and oil-based compound fragrance compositions; j8-carotene, paprika pigment, lycopene, palm oil carotene, donariella carotene and carrot Oily pigments such as carotene; vitamins A, D, Fat-soluble vitamins such as E and K; Polyunsaturated fatty acids such as docosahexaenoic acid, eicosapentaenoic acid, and _Ύlinolenic acid; _Animal and vegetable oils such as soybean oil, rapeseed oil, corn oil and fish oil; SAIB (Sucrose Acetate isobutyrate : Sucrose acetate isobutyrate) or processed food oils such as C6-C12 medium chain triglycerides and any mixture of these edible oily materials.

[0048] The method of preparing the emulsion using the modified gum arabic is not particularly limited, and the hydrophobic substance and the hydrophilic solvent are added according to a conventional method for preparing oil-in-water (OZW) type emulsion or WZOZW type emulsion. In the presence of the modified gum arabic, it can be carried out by mechanically stirring and emulsifying using a homogenizer, high-pressure jet or the like. More specifically, the following method can be illustrated.

[0049] First, the modified gum arabic is dissolved in a hydrophilic solvent such as water, and if necessary, impurities are removed by an appropriate solid-liquid separation means such as centrifugation or filtration using a filter press. A modified gum arabic aqueous solution is prepared. To this, a desired hydrophobic substance used as a dispersoid (for example, oil and fat, or a mixed solution in which a fragrance or a pigment is previously dissolved in oil or fat) is mixed with a stirrer and pre-emulsified. At this time, the specific gravity may be adjusted with a specific gravity adjusting agent such as SAIB as necessary. Next, the obtained pre-emulsified mixture is emulsified using an emulsifier.

[0050] Here, when an emulsified fragrance or an emulsified dye is prepared using an oily fragrance or an oily dye as the hydrophobic substance, a mixed solution in which the oily fragrance or the oily dye is previously dissolved in an oil or fat is used as the hydrophobic substance. It is preferable. As a result, the emulsification can be further stabilized and the volatilization of the components can be prevented. Oils and fats that dissolve oily fragrances and oily pigments are not particularly limited, but usually, medium chain triglycerides (triglycerides having 6 to 12 carbon atoms) and vegetable oils such as corn oil, safflower oil, or soybean oil are used. Can be used.

[0051] The emulsifier used for emulsification can be appropriately selected according to the size of the target emulsion particles, the viscosity of the sample, and the like, which are not particularly limited. For example, in addition to a mechanically high pressure homogenizer, an emulsifier such as a nanomizer or disperser mill or colloid mill can be used.

[0052] In the emulsification step, as described above, a hydrophobic substance is added to a hydrophilic solvent under stirring, and the mixture is stirred and pre-emulsified to prepare emulsified particles having a particle size of about 2 to 5 μm. And then homogenize Fine and uniform particles (e.g., average particle size) using an emulsifier such as

1 μm or less, preferably 0.8 μm or less). Many pigments such as β-strength rotins themselves exist as suspensions in the crystalline state. Therefore, in order to prepare these dyes as emulsions (emulsified dyes), it is preferable that the dye crystals are first mixed with an appropriate oil and fat at high temperature and dissolved, and then added to a hydrophilic solvent.

[0053] In the preparation of emulsion, the ratio of the modified gum arabic used is not particularly limited, but the ratio in 100% by weight of emulsion usually includes 5 to 20% by weight.

[0054] Thus, the emulsion (emulsion composition) prepared using the modified gum arabic has a particle size smaller than that of the emulsion prepared using ordinary natural gum arabic [unmodified gum arabic]. The distribution is uniform, and the emulsion particles are agglomerated or coalesced by heating and long-term storage, etc., and the deterioration of the particles is significantly suppressed, providing high stability over time. RU

[0055] The food made using the emulsion prepared using the modified gum arabic is not particularly limited. For example, milk drink, lactic acid bacteria drink, carbonated drink, fruit drink, powder drink, sports drink, tea Beverages such as beverages and green tea beverages; puddings such as custard pudding and milk pudding; desserts such as jelly, bavaroa and yogurt; frozen confectionery such as milk ice cream and ice candy; chewing gum and bubble gum gum; Coated chocolate such as marble chocolate, chocolate such as melon chocolate, etc .; caramels such as node candy, soft candy, caramel, and drop; node biscuits, cookies, and okaki Baked goods such as corn soup Sauces such as pork and potage soup, dressings, ketchup, mayonnaise, sauce, sauces and other sauces; processed meat products such as sausages, sausages and grilled pork; fish paste products such as fish sausages and salmon; butter, margarine, Fats and oils such as cheese Examples of processed foods such as products.

Example

Hereinafter, the present invention will be described with reference to test examples and examples. However, the present invention is not limited to these examples. It is not limited to such as. In the following experimental examples, the absorbed dose was measured using a CTA dosimeter (FTR-125, Fuji Photo Film Co. Ltd) calibrated with a Fricke dosimeter.

(1) Preparation of test sample

(1-1) Spray-dried powdered gum arabic [S§M, MW 600,000, loss on drying 7.0%] (Sample 1) in a 0.04 mm thick polyethylene bag (New Pack G-8, Produced by Nippon Stock Co., Ltd. (140 mm wide x 200 mm long), put 50 g so that the thickness of the sample is about 3 mm, make it uniform, and use a vandegraph electron accelerator to accelerate voltage 0.8 MeV, dose rate 60 Electron beams were irradiated with absorbed doses of 10, 20, 30, and 50 kGy under the conditions of OkGyZhr (electron beam irradiation sample 1).

[0058] (1-2) a spray-dried powdered gum arabic (A.Smigsal, MW60 ten thousand, dried down weight 7.0%) was blended in ion-exchanged water, the concentration of gum arabic 20 weight ⁰ An aqueous solution of gum arabic was prepared so as to be / ₀ (sample 2), 35 wt% (sample 3), 50 wt% (sample 4), and 60 wt% (sample 5). 75 g of the sample water solution is placed in a polyethylene bag with a thickness of 0.04 mm (manufactured by Nippon Co., Ltd .: 140 mm wide x 200 mm long) so that the thickness is about 3 mm. Then, using a van der graph electron accelerator, an electron beam is absorbed with an absorbed dose of 10, 20, 30, and 50 kGy for each sample (samples 2 to 5) under the conditions of an acceleration voltage of 0.8 MeV and a dose rate of 600 kGyZhr. Each was irradiated (electron beam irradiated samples 2 to 5).

[0059] (2) Physical property evaluation

For the electron beam irradiated samples 1 to 5 prepared above, (i) the weight average molecular weight, (ii) the degree of coloring, and (iii) the viscosity were evaluated according to the methods described below.

[0060] (i) Weight average molecular weight

The weight average molecular weight of gum arabic is measured by gel filtration chromatography with three detectors connected online, a light scattering tongue (MALLS) detector, a refractive index (RI) detector, and a UV detector. (GPC-MALLS) can be obtained by measuring with the ASTRA Version 4.5 (Wyatt Technology) software. According to the GPC-MALLS method, light scattering (MALL S) The molecular weight can be detected by the detector, the weight (composition ratio) of each component can be detected by the refractive index (RI) detector, and the protein can be detected by the UV detector. The molecular weight and composition of the analytical component can be determined without any problem. Its detailed principles and features are described in Idris, OHM, Williams, PA, Phillips, GO; Food Hydrocolloids, 12,375-388 (1998) J.

[0061] The measurement conditions of GPC-MALLS employed in the present invention are as follows:

Kahum: buperose 6 10/300 uL, mersham Biosciences, Sweden;

Flow rate: 0.5ml / min

Elution solvent: 0.2M NaCl

Sample preparation: After the analysis sample (electron beam irradiated sample) is diluted with the elution solvent (0.2 M NaCl), measure the solution from which insolubles have been removed with a 0.45 m cellulose acetate membrane filter.

Sample concentration: 0.1% (WZV)

Sample solution injection volume: 100 μ \

dn / dc: 0.141

Temperature: Room temperature

Detector: 1) MALLS (multi angle laser light scattering): DAWN EOS (Wyatt Technology, USA), 2) RI (refractive index), 3) UV (absorption at 214 nm).

[0062] Under the above conditions, the entire peak on the chromatogram obtained by the RI detector (when the rising edge of the RI chart from the baseline of the chart starts and descends and the intersection with the baseline is the end point) The starting force also means the RI chart part to the end point.) When the data was processed as one peak (processed as one peak), the molecular weight determined by weight conversion was defined as the “weight average molecular weight”. As the emulsifying power increases, the “weight average molecular weight” also increases, which can be used as an index for evaluating emulsifiability.

[0063] (ii) Degree of coloring

For sample 1 irradiated with electron beam, 90 g of ion-exchanged water was added to 10 g of the sample and mixed well to prepare a 10% by weight aqueous solution of gum arabic. Electron beam irradiated samples 2 to 5 were prepared using ion-exchanged water so that the gum arabic concentration was 10% by weight. [0064] These solutions (each sample irradiated with an electron beam adjusted as a 10% by weight aqueous solution) were placed in a quartz cell having an optical path length of 10 mm, and an ultraviolet-visible spectrophotometer V-560 (manufactured by JASCO Corporation) was used. Measured with transmitted light, the tristimulus value of the Hunter color system (Lab color system) was determined, and (a ² + b ² ) was calculated as the coloring degree.

[0065] (m) Viscosity

For electron beam irradiated sample 1, 90 g of ion exchange water was added to sample lOg and mixed well to prepare a 10% by weight aqueous solution of gum arabic. Electron beam irradiated samples 2 to 5 were prepared using ion-exchanged water so that the gum arabic concentration was 10% by weight.

[0066] Each electron beam irradiation sample prepared as a 10% by weight aqueous solution as described above was placed in a 100 ml screw tube, and a B-type viscometer (BM type, Tokimec Co., Ltd.) at 20 ° C. ), The viscosity (mPa's) was measured under the conditions of rotor No. 1 and rotation speed of 60 rpm. (For samples that cannot be measured with rotor No. 1, use rotor No. 2 with the same rotation speed of 60 rpm.) Viscosity (mPa · s) was measured under the conditions).

[0067] Table 1 shows the results of the above measurements. Figures 1, 2 and 3 show the changes in molecular weight, viscosity, and coloring degree when each sample (Samples 1-5) was irradiated with an electron beam of each absorbed dose (0-50 kGy).

[0068] [Table 1]

Changes in physical properties of samples irradiated with electron beam

[0069] As a result, for sample 1 irradiated in the powder state, the molecular weight and viscosity decreased as the absorbed dose increased, indicating that gum arabic was decomposed by electron beam irradiation.

[0070] On the other hand, 20% aqueous solution, 35% aqueous solution, 50% aqueous solution, and 60% aqueous solution of gum arabic (electron-irradiated sample 25) were all irradiated with an electron beam. Since increases in molecular weight and viscosity were observed, it is considered that gum arabic was not decomposed but polymerized.

[0071] Regarding the degree of coloring, 20% by weight aqueous solution, 35% by weight aqueous solution, 50% by weight aqueous solution, and 60% by weight aqueous solution compared to the case where powdered (solid) gum arabic is irradiated with an electron beam. When an object (electron-irradiated sample 25) is irradiated, it is recognized that coloring is significantly low, and coloring is suppressed most particularly in 35 wt% aqueous solution and 50 wt% aqueous solution. Was suppressed. This proved that irradiation in a 3055 wt% aqueous solution concentration range is more useful for the preparation of modified gum arabic with suppressed coloration. [0072] Experimental Example 2

(1) Preparation of test sample

Add 65 kg of ion-exchanged water heated to 90 ° C to 35 kg of gum arabic (A. SeneeaL MW 600,000, loss on drying 7.0%), and mix by stirring. After removing the insoluble matter with a centrifuge, 35% by weight of an aqueous solution of gum arabic was obtained as the supernatant. 1.5 kg of this was put into a 2 L stainless steel container, and γ-rays were irradiated with cobalt 60 at an absorbed dose of 2.5, 5, 10, 20, 30, 40, and 50 kGy (dose rate lkGyZhr).

[0073] (2) Physical property evaluation

For each of the samples obtained above, the weight average molecular weight, viscosity, and coloring degree were examined according to the method of Experimental Example 1. The results are shown in Table 2.

[0074] [Table 2]

Changes in physical properties of 35% aqueous solution of gum arabic irradiated with r-rays

[0075] (3) Emulsifying power evaluation

Each sample obtained above was evaluated for emulsifying power according to the following method. <Method>

Attachment Caro combined mixed ion exchange water 300g into γ-ray irradiation was 35 weight ^_0/0 arabic gum aqueous was 500 g, while stirring the solution, 0. D. is a medium-chain triglyceride (octanoic 'decanoic acid triglyceride) 0 (Trade name, manufactured by Nisshin Oillio Co., Ltd.) Add 200g, mix and emulsify with homogenizer (manufactured by APV GAULIN) to prepare emulsion (4 times homogenization at 44MPa (450kg / cm ² )).

[0076] For the obtained emulsion !, the average particle size m) immediately after emulsification and after storage at 60 ° C for 3 days was measured using a particle size distribution analyzer SALD-1100 (laser diffraction type, manufactured by Shimadzu Corporation). Use to measure. In general, the emulsifying power of an emulsifier is equal to the level of the emulsion to be prepared. It is estimated that the smaller the average particle size and the more stable the particle size over time, the better it will be (“Study by the turbidity ratio method of o / w emulsion emulsified with gum arabic”). "Pharmacological Journal, 112 (12) 906-913, (1992)). Emulsification stability was also evaluated by the difference in average particle size immediately after emulsification and after storage at 60 ° C for 3 mm.

[0077] Table 3 shows the evaluation results of emulsifying power. The stability of emulsion over time (emulsification stability) is the difference between the average particle diameter (a) of emulsion just after emulsification and the average particle diameter (b) of emulsion after accelerated test (stored at 60 ° C for 3 days). (b— a) Force can also be judged. The smaller the change in the average particle size of the emulsion, the better the stability with time (emulsification stability).

[0078] [Table 3]

[0079] As shown in the above results, the emulsion prepared using an unmodified gum arabic aqueous solution without y-irradiation was subjected to an accelerated test (stored at 60 ° C for 3 days). As the emulsion particle force decreased, the average particle size changed from 1.65 μπι to 1.85 μπι to 1.85 μπι. In contrast, emulsions prepared using gamma-irradiated gum arabic aqueous solution showed significantly smaller changes in average particle size in accelerated tests (60 ° C, stored for 3 days), and stability over time ( Emulsification stability was improved.

[0080] In particular, in emulsions prepared using gum arabic aqueous solution irradiated with ionizing radiation at 20 kGy or more, there was almost no change in the average particle size due to the accelerated test (stored at 60 ° C for 3 days). High emulsification stability was exhibited. A small amount of gel was observed in the aqueous gum arabic solution irradiated with 50 kGy. Therefore, the absorbed dose for obtaining a suitable water-soluble modified gum arabic with excellent emulsifiability was considered to be preferably 50 kGy or less. [0081] Among the above experimental examples, an aqueous gum arabic solution irradiated with γ rays at 30 kGy was dried and used as modified gum arabic in the following examples.

[0082] Example 1 | 8—Strength Rotin Emulsified Formulation (Emulsified Dye Formulation)

—Power Rotin 30% Suspension 5 (wt%)

Medium chain triglyceride 10

Modified gum arabic 17

Zt 68

Total 100% by weight

[0083] The modified gum arabic 170g was dissolved in water 680 g, it was manufactured by adjusting the aqueous gum arabic solution of 20 weight ^_0/0. This was used as a wrinkle preparation, and medium chain triglyceride (octanoic acid / decanoic acid triglyceride, OD 0 (trade name, manufactured by Nisshin Oil Co., Ltd.)) 100 g was mixed and heated at 150 ° C. to dissolve, and the mixed solution was added and mixed with stirring. This was emulsified with a homogenizer (APV GAULIN) (4 times homogenization at a pressure of 4.4 MPa (450 kgZcm ² )) to prepare an emulsified pigment preparation, j8-power rotin emulsion preparation.

[0084] Example 2 Orange emulsified flavor (emulsified flavor)

Orange flavoring 2 (weight ^_0/0)

Medium chain triglyceride 13

Modified gum arabic 17

Zt 68

Total 100% by weight

[0085] The modified gum arabic 170g was dissolved in water 680 g, it was manufactured by adjusting the aqueous gum arabic solution of 20 weight ^_0/0. Using this as an emulsifier, it was prepared by mixing in advance 20 g of orange fragrance and 130 g of medium chain triglyceride (octanoic acid / decanoic acid triglyceride, OD 0 (trade name, manufactured by Nisshin Oil Co., Ltd.)) at room temperature. The previously mixed liquid was added and mixed with stirring. This is emulsified with a homogenizer (APV GAULIN) (with a pressure of 4.4 MPa (450 kg / cm ² )). The orange emulsified fragrance was prepared as an emulsified fragrance.

Example 3 DHA (docosahexaenoic acid) emulsion formulation

Fish oil containing 20% DHA 5 (wt%)

Medium chain triglyceride 10

Modified gum arabic 17

Zt 68

Total 100% by weight

[0087] The modified gum arabic 170g was dissolved in water 680 g, it was manufactured by adjusting the aqueous gum arabic solution of 20 weight ^_0/0. Using this as an emulsifier, a mixture of 50 g of fish oil containing 20% by weight of DHA and medium chain triglyceride (octanoic acid / decanoic acid triglyceride, OD 0 (trade name, manufactured by Nisshin Oil Co., Ltd.)) lOOg was added in advance to 80 °. The mixture prepared by heating to C and mixing was added and mixed by stirring. This was emulsified with a homogenizer (manufactured by APV GAULIN) (homogenization at a pressure of 4.4 MPa (450 kg / cm ² ) four times) to prepare a DHA emulsion preparation.

[0088] Decoration 14 Lemon powder flavor

Lemon oil 20 (weight ^_0/0)

Modified gum arabic 20

Dextrin 60

2t 150

Total 250% by weight

[0089] 200 g of modified gum arabic and 600 g of dextrin are dissolved in 1500 g of water to prepare an aqueous gum arabic solution. Using this as an emulsifier, add 200 g of lemon oil and stir and mix. This is emulsified with a homogenizer (APV GAULIN) (one homogenization at a pressure of 2.0 MPa (200 kg / cm ² )). Next, this solution is spray-dried with a spray dryer (manufactured by ANHYD RO) (inlet 140.C, outlet 80.C) to prepare a lemon powder flavor 95 Og.

Claims

The scope of the claims

[I] A method for producing modified gum arabic, comprising a step of irradiating an aqueous gum arabic solution with ionizing radiation.

[2] The method for producing modified gum arabic according to claim 1, wherein the concentration of gum arabic in the gum arabic aqueous solution is 15 to 60% by weight.

[3] The method for producing a modified gum arabic according to claim 1, wherein the ionizing radiation is γ rays.

[4] The method for producing modified gum arabic according to claim 1, wherein the ionizing radiation is an electron beam having an acceleration voltage of 0.2 to: LOMeV.

[5] The method for producing modified gum arabic according to claim 1, wherein the absorbed dose of ionizing radiation is 1 to 50 kGy.

[6] 3 in gum arabic concentration 15 to 50 weight ^_0/0 to gum arabic water was irradiating ionizing radiation absorbed dose 10~50kGy or arabic gum concentration 50-60% by weight of gum arabic water of The method for producing modified gum arabic according to claim 1, comprising a step of irradiating ionizing radiation with an absorbed dose of 30 kGy.

7. The method for producing a modified gum arabic according to claim 1, further comprising a drying step after the step of irradiating the gum arabic aqueous solution with ionizing radiation.

8. The method for producing a modified gum arabic according to claim 7, wherein the drying step is spray drying or drum drying.

[9] Modified gum arabic obtained by the production method according to any one of claims 1 to 7.

[10] An emulsifier comprising the modified gum arabic according to claim 9 as an active ingredient.

[II] A method for preparing an emulsified composition using the modified gum arabic described in claim 9 as an emulsifier.

[12] The method for preparing an emulsified composition according to claim 11, wherein the emulsified composition is OZW type emulsion or WZOZW type emulsion.

[13] Essential oil, oily fragrance, oily pigment, oil-soluble vitamin, polyunsaturated fatty acid, animal and vegetable oil, sucrose acetate isobutyric acid ester, using the modified gum arabic according to claim 9 as an emulsifier 12. The method for preparing an emulsified composition according to claim 11, further comprising a step of dispersing and stabilizing at least one hydrophobic substance selected from the group force consisting of water and medium chain triglyceride in a hydrophilic solvent.

[14] An emulsified composition prepared using the modified gum arabic according to claim 9 as an emulsifier

[15] The emulsified composition according to claim 14, obtained by the preparation method according to claim 11.

[16] The emulsified composition according to claim 14, which is a food or drink.