WO2006032504A3 - Procede permettant d'introduire des mutations genetiques dans des cellules vegetales - Google Patents

Procede permettant d'introduire des mutations genetiques dans des cellules vegetales Download PDF

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Publication number
WO2006032504A3
WO2006032504A3 PCT/EP2005/010270 EP2005010270W WO2006032504A3 WO 2006032504 A3 WO2006032504 A3 WO 2006032504A3 EP 2005010270 W EP2005010270 W EP 2005010270W WO 2006032504 A3 WO2006032504 A3 WO 2006032504A3
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WIPO (PCT)
Prior art keywords
present
plant
genetic mutations
plant cell
plant cells
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Application number
PCT/EP2005/010270
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English (en)
Other versions
WO2006032504A2 (fr
Inventor
Gabriela Ispas
Ivan Famelaer
Geert Angenon
Original Assignee
Univ Bruxelles
Gabriela Ispas
Ivan Famelaer
Geert Angenon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bruxelles, Gabriela Ispas, Ivan Famelaer, Geert Angenon filed Critical Univ Bruxelles
Publication of WO2006032504A2 publication Critical patent/WO2006032504A2/fr
Publication of WO2006032504A3 publication Critical patent/WO2006032504A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention se rapporte à un procédé permettant d'introduire des mutations génétiques dans le génome de cellules végétales. Le procédé selon l'invention comprend les étapes consistant : (i) à supprimer l'activité d'au moins un système endogène de réparation de l'ADN dans au moins une cellule végétale ; (ii) à exposer ladite cellule végétale à au moins un agent mutagène. Certains modes de réalisation consistent à supprimer le système de réparation du mésappariement dans ladite cellule végétale, et à utiliser un agent d'alkylation de l'ADN pour la mutagenèse. L'invention a pour but de permettre la génération de banques de mutants de diverses espèces végétales, dans lesquelles une proportion plus importante de gènes présentera une mutation et un nombre plus important de phénotypes différents sera obtenu que ce qu'il est possible de réaliser à l'aide des procédés actuels. La présente invention aura pour effet de combler la 'lacune phénotypique' touchant les banques existantes de mutants végétaux.
PCT/EP2005/010270 2004-09-22 2005-09-22 Procede permettant d'introduire des mutations genetiques dans des cellules vegetales WO2006032504A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2004010617 2004-09-22
EPPCT/EP2004/010617 2004-09-22

Publications (2)

Publication Number Publication Date
WO2006032504A2 WO2006032504A2 (fr) 2006-03-30
WO2006032504A3 true WO2006032504A3 (fr) 2006-07-13

Family

ID=36084413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010270 WO2006032504A2 (fr) 2004-09-22 2005-09-22 Procede permettant d'introduire des mutations genetiques dans des cellules vegetales

Country Status (1)

Country Link
WO (1) WO2006032504A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101597328B (zh) * 2008-06-06 2012-07-11 中国科学院遗传与发育生物学研究所 促进植物体细胞胚胎发生和脂肪酸合成的转录因子及其编码基因与应用
WO2010074562A1 (fr) * 2008-12-22 2010-07-01 Keygene N.V. Utilisation de l'arn à double brin de manière à augmenter l'efficacité d'une altération génétique ciblée à l'intérieur de protoplastes végétaux
EP2516652B1 (fr) * 2009-12-21 2014-11-05 Keygene N.V. Techniques améliorées de transfection de protoplastes
CN102812803B (zh) * 2012-07-02 2016-02-24 青岛市农业科学研究院 一种豌豆ems突变体库构建的方法
US10595479B2 (en) * 2014-04-24 2020-03-24 Purdue Research Foundation Induced mutagenesis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005268A1 (fr) * 1995-07-26 1997-02-13 Setratech Recombinaison homologue dans des cellules eucaryotes a systeme de reparation des mesappariements inactive
WO1999019492A2 (fr) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Procedes pour obtenir des varietes de plantes
WO2001059092A2 (fr) * 2000-02-11 2001-08-16 The Johns Hopkins University Procedes d'obtention de microbes hypermutables
WO2001061012A1 (fr) * 2000-02-18 2001-08-23 Nicolaides Nicholas C Procede de generation de plantes tres sujettes aux mutations
WO2001088192A2 (fr) * 2000-05-12 2001-11-22 The Johns Hopkins University Procede de generation d'organismes a mutabilite tres elevee
WO2002054856A1 (fr) * 2001-01-15 2002-07-18 Morphotek Inc. Inhibiteurs chimiques de reparation de desappariements
WO2003054217A2 (fr) * 2001-12-20 2003-07-03 Boyce Thompson Institute For Plant Research Sequences msh2 de plantes et methodes d'utilisation correspondantes

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005268A1 (fr) * 1995-07-26 1997-02-13 Setratech Recombinaison homologue dans des cellules eucaryotes a systeme de reparation des mesappariements inactive
WO1999019492A2 (fr) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Procedes pour obtenir des varietes de plantes
WO2001059092A2 (fr) * 2000-02-11 2001-08-16 The Johns Hopkins University Procedes d'obtention de microbes hypermutables
WO2001061012A1 (fr) * 2000-02-18 2001-08-23 Nicolaides Nicholas C Procede de generation de plantes tres sujettes aux mutations
WO2001088192A2 (fr) * 2000-05-12 2001-11-22 The Johns Hopkins University Procede de generation d'organismes a mutabilite tres elevee
WO2002054856A1 (fr) * 2001-01-15 2002-07-18 Morphotek Inc. Inhibiteurs chimiques de reparation de desappariements
WO2003054217A2 (fr) * 2001-12-20 2003-07-03 Boyce Thompson Institute For Plant Research Sequences msh2 de plantes et methodes d'utilisation correspondantes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ADE JULES ET AL: "Functional analysis of the Arabidopsis thaliana mismatch repair gene MSH2", GENOME, vol. 44, no. 4, August 2001 (2001-08-01), pages 651 - 657, XP001208017, ISSN: 0831-2796 *
ANDREW SUSAN E ET AL: "Tissues of MSH2-deficient mice demonstrate hypermutability on exposure to a DNA methylating agent", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, no. 3, 3 February 1998 (1998-02-03), pages 1126 - 1130, XP002375421, ISSN: 0027-8424 *
HOFFMAN PETER D ET AL: "Rapid accumulation of mutations during seed-to-seed propagation of mismatch-repair-defective Arabidopsis", GENES & DEVELOPMENT, vol. 18, no. 21, 1 November 2004 (2004-11-01), pages 2676 - 2685, XP002375419, ISSN: 0890-9369 *
ISPAS GABRIELA ET AL: "Interferance of Mismatch Repair System on genetic recombination in plants.", MEDEDELINGEN FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN UNIVERSITEIT GENT, vol. 67, no. 4, 2002, pages 239 - 241, XP008062435, ISSN: 1373-7503 *
LEONARD JEFFREY M ET AL: "Reduction of stability of Arabidopsis genomic and transgenic dna-repeat sequences (Microsatellites) by inactivation of AtMSH2 mismatch-repair function.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 133, no. 1, September 2003 (2003-09-01), pages 328 - 338, XP002375417, ISSN: 0032-0889 *
RAKOSY-TICAN LENUTA ET AL: "Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes", PLANT BREEDING AND SEED SCIENCE, vol. 50, 2004, pages 119 - 127, XP008062469, ISSN: 1429-3862 *
STUDAMIRE BARBARA ET AL: "Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination", MOLECULAR AND CELLULAR BIOLOGY, vol. 19, no. 11, November 1999 (1999-11-01), pages 7558 - 7567, XP002375528, ISSN: 0270-7306 *

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