WO2006032171A1 - Pompe électro-osmotique à microflux à membrane ionique - Google Patents
Pompe électro-osmotique à microflux à membrane ionique Download PDFInfo
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- WO2006032171A1 WO2006032171A1 PCT/CN2004/001294 CN2004001294W WO2006032171A1 WO 2006032171 A1 WO2006032171 A1 WO 2006032171A1 CN 2004001294 W CN2004001294 W CN 2004001294W WO 2006032171 A1 WO2006032171 A1 WO 2006032171A1
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- WIPO (PCT)
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- exchange membrane
- electroosmotic
- channel
- pump
- cation exchange
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Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
- F04B19/006—Micropumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B17/00—Pumps characterised by combination with, or adaptation to, specific driving engines or motors
Definitions
- the invention relates to a method for capillary liquid chromatography, micro ion chromatography, micro flow injection analysis system, micro total analysis system, micro flow analysis system, especially in the field of chip laboratory, micro flow transport and the like.
- the percolation drive pump infusion device is not limited to capillary liquid chromatography, micro ion chromatography, micro flow injection analysis system, micro total analysis system, micro flow analysis system, especially in the field of chip laboratory, micro flow transport and the like.
- Micro flow injection analysis (FIA) systems and chip capillary electrophoresis (CE) have been developed.
- Systems, micro-gas chromatography (GC), micro-mass spectrometry (MS) and micro-spectrometers, etc. have recently received increasing attention in micro-liquid chromatography (LC).
- LC micro-liquid chromatography
- ⁇ -TAS micro total analysis system
- the miniaturization of analytical instruments not only reduces the consumption of samples and reagents to microliters or even nanoscales, but also increases the speed of analysis by tens or even hundreds of times.
- the processing capacity of batch samples is greatly enhanced, and more importantly, the cost of analysis can be reduced. Hundreds of times, while greatly reducing environmental pollution.
- MEMS microelectromechanical processing technology
- micro-flow separation analysis systems generally require a liquid flow rate of 50 nL/min ⁇ 5 ( ⁇ IJmin, and the flow rate and pressure are controllable, the mobile phase composition and flow direction are controllable, and some systems require high-pressure infusion, generally requiring a pressure of 3 to 10 MPa. .
- infusion micropumps are mechanical pumps such as piston reciprocating pumps, syringe pumps, diaphragm pumps, peristaltic pumps, and planetary gear pumps. These mechanical pumps are driven by mechanical forces, require high-precision moving parts, require high-strength, corrosion-resistant materials, and are relatively expensive to manufacture. Even so, it is still difficult to avoid the leakage of liquids caused by wear or corrosion of the dynamic sealing parts for long-term use.
- the leakage of dynamic seals is generally above ⁇ . ⁇ , and it is uncontrollable, therefore, to get The flow rate is less than 2 ⁇ / ⁇ , and the mechanical pump with a pressure of 3MPa or more is very difficult.
- the piston pump and the reciprocating pump are inevitably present. Hydraulic fluctuations.
- these micropumps are expensive to manufacture and are significantly less reliable at low flow rates. So far, there has not been a micromechanical pump capable of providing a stable and accurate flow rate lower than ⁇ 7 ⁇ above a pressure of 3 MPa.
- electrohydrodynamic magnetohydrodynamic
- electroosmotic electroosmotic pump
- electroosmotic pump has the most practical potential, because only the electroosmotic micropump can pump high pressure fluid, and the output pressure of other types of micropumps is very low.
- the electroosmotic pump is a principle of electroosmotic driving using a current carrying current, that is, in the case where the inner wall of the channel or the surface of the filling particle and the liquid medium in the vicinity thereof have different symbols of electric charge to form an electric double layer, the surface charge of the solid inner wall or the filling cannot be moved, and In the diffusion layer of the electric double layer, the carrier current with a different electric charge in the liquid medium moves under the action of the external electric field, and drags the surrounding liquid to move together to form an electroosmotic flow.
- ⁇ - ⁇ open-tube electroosmotic pumps
- ⁇ - ⁇ packed bed electroosmotic pumps
- ⁇ - ⁇ the flow rate is not stable enough and the output pressure is low, generally less than 100 cm water column static pressure.
- p-EOP mainly uses the granular dielectric filler filled in the capillary channel to increase the reverse flow resistance of electroosmotic flow and liquid, and increase the output pressure.
- high pressure output pressure up to 50MPa
- electroosmotic pumps have high driving voltages, generally several kV, or even tens of kV, requiring strict electrical insulation measures and electrical isolation measures, and the safety is not high, which is not conducive to the miniaturization of the instruments.
- driving voltages generally several kV, or even tens of kV
- the pressure of the output liquid is relatively low, and there are other disadvantages, such as the porous core electroosmotic pump (CN2286429Y) disclosed by the University of Science and Technology of China, whose working voltage is 10 ⁇ 500V, but it is large in size. It needs to stop pumping regularly to discharge the gas generated by the electrode. It can't work continuously for a long time, and its life is limited.
- the electrode when the electrode reacts to generate gas, it will cause the fluid pH in the electrode cavity.
- the chip-type micro-flow electroosmotic pump (CN1419954A) disclosed by the Dalian Institute of Chemical Physics of the Chinese Academy of Sciences has a driving voltage of 5 ⁇ 200V, but it can only produce pressures up to 700kPa, and the structure is more complicated.
- a special degassing device to eliminate air bubbles generated by electrolysis on the electrodes.
- the generation of Joule heat limits the performance of the pump. Since the electroosmotic channels used in the literature are all quartz or quartz capillaries, it is difficult to install a diffuser device on the outside to remove the Joule heat generated during energization, and the higher the voltage, the more pronounced the Joule heat is. When the electric field strength is greater than lOOOV/cm, the Joule heat generated will seriously affect the output performance of the pump, and even cause water vaporization to form bubbles, causing the electric passage to open and electroosmosis to stop.
- electroosmotic pumps there are many factors affecting the performance of electroosmotic pumps. Since the generation of electroosmotic flow is closely related to many factors such as the dielectric constant, viscosity, composition and concentration of the fluid, pH, and zeta potential of the filler particles, it is necessary to strictly control the type and concentration of the electrolyte and the pH in the fluid. Prevent the entry of impurities, which may result in electro-osmotic flow or changes in the direction of fluid drive. What is more troublesome is that the pumping liquid is different, and the output performance of the electroosmotic pump is different; even the same liquid may occur during use due to the physical properties of the liquid itself or the physical properties of the pump material.
- the change causes the output flow of the electroosmotic pump to change, which means that the output flow accuracy and stability are not high. These factors can cause great inconvenience in the actual use of the electroosmotic pump, and the output pressure and flow rate of the electroosmotic pump become difficult to control. .
- Electroosmotic pump is a kind of micro-flow infusion pump with great application value. Its superiority in high-pressure micro-flow infusion far exceeds that of mechanical micro-pump, but these defects of existing electroosmotic pumps greatly limit its application. There are still no mature products yet.
- the invention solves the problem that the electroosmotic pump is difficult to manufacture, has high cost, is not easy to dissipate heat during operation, has unstable output performance, and is difficult to regulate output pressure and flow, and the generation of bubbles seriously affects the output performance of the electroosmotic pump and the output of the pump.
- There are problems such as bubbles and electric fields at the ends, and an ion-exchange membrane micro-flow electroosmotic pump of the present invention is provided for this purpose.
- the technical solution adopted by the present invention is to provide a channel I and a channel II, and a cation exchange membrane is encapsulated in the channel I, both ends of which are exposed at both ends of the channel I, and the channel II is encapsulated with an anion exchange film at both ends thereof.
- a positive electrode pool containing the positive electrode electrolyte and a negative electrode pool containing the negative electrode electrolyte Exposed at both ends of the channel II, there is a positive electrode pool containing the positive electrode electrolyte and a negative electrode pool containing the negative electrode electrolyte, one end of the channel I is placed in the positive electrode pool, and one end of the channel II is placed in the negative electrode pool, and an outlet pump chamber is provided. The other end of the channel I and the other end of the channel II are connected to the pump chamber.
- the cation exchange membrane and the anion exchange membrane exposed at the end of the channel I and the channel II are electrically connected to each other in the pump chamber, and the positive electrode pool is interposed with The positive electrode column connected to the positive terminal of the driving power source is inserted into the negative electrode pool with a negative electrode column connected to the negative electrode end of the power supply.
- the present invention can be provided with a buffer tank having an inlet and an outlet, A capillary is connected between the outlet of the pump chamber and the inlet of the buffer tank, and a diaphragm is provided between the inlet and the outlet of the buffer tank.
- the diaphragm should be liquid-tight and should have considerable flexibility and resistance. Ten strength. The liquid on the outlet side is delivered through the pressure transmission after the diaphragm is pressurized.
- the channel I, the channel II of the present invention may be a thin-walled plastic tube, such as a Teflon tube, a polyethylene tube or other plastic hose, or may be micro-machined on a chip or on quartz or borosilicate glass. Into the channel.
- a thin-walled plastic tube such as a Teflon tube, a polyethylene tube or other plastic hose, or may be micro-machined on a chip or on quartz or borosilicate glass.
- the channel on the chip refers to the material transport channel in the "lab on a chip"; the chip laboratory is essentially a microreactor or microseparation analysis system.
- the cation exchange membrane may be a perfluorosulfonic acid cation exchange membrane, a perfluorocarboxylic acid cation exchange membrane, a fluorosulfonic acid cation exchange membrane, a fluorocarbon cation exchange membrane, a polyethylene homogeneous cation exchange membrane, and a coating.
- One of the polyethylene homogeneous cation exchange membranes are preferred.
- preferred are perfluorosulfonic acid cation exchange membranes, fluorosulfonic acid cation exchange membranes, and most preferred are perfluorosulfonic acid cation exchange membranes, the most famous of which is DuPont's Nafion® 100 series membrane.
- the anion exchange membrane may be one of a fluorocarbon anion exchange membrane, a polystyrene anion exchange membrane, a polymethacrylic anion exchange membrane, a polyethersulfone anion exchange membrane or a polycrown ether anion exchange membrane.
- a fluorocarbon anion exchange membrane a polystyrene anion exchange membrane, a polymethacrylic anion exchange membrane, a polyethersulfone anion exchange membrane or a polycrown ether anion exchange membrane.
- NF 201 anion exchange membrane is the NF 201 anion exchange membrane.
- the cation exchange membrane and the anion exchange membrane may have a suitable width of between 0.1 and 500 mm.
- a heat sink member may be disposed on the outer wall of the channel I and the channel II; the heat sink member may be coated with a metal heat sink on the outer wall of the channel, or Place the outer wall of the channel in a constant temperature liquid.
- the invention encapsulates a cation exchange membrane and an anion exchange membrane in the channel I and the channel II respectively; the channel is sleeved, and the end of the sleeve is sealed with glue but the ionic membrane is exposed to protrude from the end of the sleeve 0-100 mm
- the cation exchange membrane and the anion exchange membrane exposed at the end of the channel I and the channel II and the pump chamber are electrically contacted with each other in the pump chamber, and may be directly contacted with each other by electrical contact, or may be realized by an electrolyte or an anion-cation mixed ion exchange resin. Electrical contact.
- the driving power source connected to the positive electrode column and the negative electrode column is a DC power source, and a suitable voltage is 3 to 500V.
- the invention is driven by the relatively low voltage, and can generate electroosmotic flow from the milliliter level to the nano upgrade, and even the skin upgrade, the output pressure is 0.01-50 MPa, the flow rate is precisely adjustable, and the flow rate is stable and reliable.
- one end of the cation exchange membrane 1 and the anion exchange membrane 2 is inserted into the pump chamber 9 and sealed. Their other ends are immersed in the positive electrode pool 4 and the positive electrode electrolyte 5 in the negative electrode cell, respectively, and negative.
- a positive electrode column 3 and a negative electrode column 6 are inserted into the positive electrode cell 4 and the negative electrode cell 7, respectively.
- the positive electrode column 3 and the negative electrode column 6 are respectively connected to the positive electrode and the negative electrode of a direct current power source (not shown).
- the working principle of the electroosmotic pump is described by taking Nafion® cation exchange membrane, NF201 anion exchange membrane, positive electrolyte as dilute sulfuric acid solution and negative electrolyte as dilute sodium hydroxide solution.
- the Nafion® membrane is also called proton exchange membrane (PEM) when its cation is H+.
- the membrane itself consists of a hydrophobic body and a hydrophilic ion cluster.
- the former is mainly composed of a polytetrafluoroethylene skeleton, and the hydrophilic ion clusters have a diameter of about 50 to 60 ⁇ . 'These ion clusters are connected to each other through a channel having a diameter of about 10 to 20 ⁇ .
- These hydrophilic ion clusters and their channel inner walls are connected to each other.
- a negatively charged sulfonic acid group is arranged, the sulfonic acid group is attached to the bulk, and the position is fixed.
- the hydrophilic cluster contains cations with an equal amount of positive charges to maintain electrical neutrality, and further, a solvating solvent molecule and Some free solvent molecules, because the positive electrode electrolyte is a dilute sulfuric acid aqueous solution, correspondingly, the hydrophilic cluster contains hydrogen ions, solvated water molecules and free water molecules.
- a DC electric field passes through the hydrophilic clusters in the membrane and its channels.
- the sulfonic acid group is immobilized on the hydrophobic polytetrafluoroethylene skeleton, and only hydrogen as a carrier. Ions, hydrogen ions will move toward the negative electrode under the action of an electric field, and drag their own solvated water molecules together to form a positive electroosmotic flow.
- the NF201 anion exchange membrane is also similar, and the membrane itself is also composed of a hydrophobic body and a hydrophilic ion cluster. Unlike the cation exchange, the hydrophilic ion cluster and the inner wall of the channel are arranged with a positively charged quaternary amine group, which is connected to the body and has a fixed position. In order to maintain electrical neutrality, the cluster also contains anions with equal negative charges and their solvated solvent molecules as well as free solvent molecules. Since the negative electrode electrolyte is a dilute aqueous sodium hydroxide solution, the cluster contains only hydroxide ions and solvated water molecules and free water. When an electric field acts, the hydroxide ions acting as carriers move their solvated water molecules toward the positive electrode in a directed manner, thereby forming a negative electrode electroosmotic flow.
- V - molar volume of solvent, mL / mol, when the solvent is water, 18 m! Vmol.
- volumetric flow per unit time of the electroosmotic flow generated by the electromigration of hydroxide ions in the NF201 anion exchange membrane by the drive current is:
- V, —xn 2 V 60 (uL/min) (2) F
- the solvation number is increased by 1 because one H+ and one OH- neutralized one molecule of water. .
- the number of hydration is about 4, which may actually be slightly smaller than 4, so 0.1.
- electroosmotic flow rate per unit time is proportional to the current intensity passed, independent of other parameters, and the influencing factors are much less than other electroosmotic pumps.
- This is the electroosmotic pump of the present invention.
- Adjusting the current through the electroosmotic pump can adjust the electroosmotic flow rate, and it is independent of the concentration and type of the positive electrode electrolyte and the negative electrode electrolyte in a wide range of electrolyte concentration, and has nothing to do with the material properties of the electroosmotic pump.
- the design and use of electroosmotic pumps brings great convenience and is also suitable for mass production.
- the positive electrode electroosmotic flow is In addition to H+, which also contains Na + , and the negative electrolyte is still pure alkaline electrolyte, the OH contained in the electroosmotic flow of the negative electrode cannot be completely neutralized by H+ in the electroosmotic flow of the positive electrode, so the pump is discharged. Alkaline. Moreover, the higher the Na+ content in the positive electrode electrolyte, the higher the Na+ content in the electroosmotic flow of the positive electrode, and the stronger the alkalinity of the pump liquid.
- the negative electrode electroosmotic flow contains Cl- in addition to OH-
- the positive electrode electrolyte is still a purely acidic electrolyte, and the H+ in the positive electrode electroosmotic flow. In other words, it will not be neutralized by OH_ in the electroosmotic flow of the negative electrode, and the pump liquid will be acidic.
- the Na+ content in the positive electrode electrolyte is continuously adjusted from low to high, the alkalinity of the pump liquid continues to increase, and the C1-content in the negative electrode electrolytic cell is continuously adjusted from low to high, and the acidity of the pump liquid continues. Enhanced.
- Na+ is added to the positive electrolyte at the same time, Cl— is added to the negative electrolyte, and the appropriate ratio of the two is controlled, so that the H+ in the electroosmotic flow of the positive electrode is completely neutralized with the OH in the electroosmotic flow of the negative electrode, and thus the positive electrode is electrically
- the Na+ in the percolation also appears exactly in the pumping liquid with the Cr in the electroosmotic flow of the negative electrode, so that the pumping liquid is maintained at a certain ionic strength. If the Na + of the positive electrolyte and the Cl— in the negative electrolyte are continuously and proportionally adjusted from low to high, the pumping liquid whose ionic strength continuously changes from low to high can be obtained at the pump chamber outlet.
- the pump solution will also contain a certain concentration of polar organic solvent.
- the more polar organic solvent added to the positive electrode electrolyte or the negative electrode electrolyte the organic in the pump solution. The higher the solvent content, if the ratio of the organic solvent to the electrolyte is continuously adjusted from low to high, the pumping liquid in which the content of the organic solvent continuously changes from low to high can be obtained.
- the electroosmotic pump provided by the invention can pump pure water, salt solution and a mixture of water and polar organic solvent, and can realize pH gradient output, gradient output of ionic strength and ratio of water to organic solvent. Gradient output, so that the electroosmotic pump is easy to use with instruments such as FIA, CE and HPLC.
- the anion exchange membrane and the cation exchange membrane are good conductors for ion conduction.
- the lower driving voltage can generate a large electroosmotic flow.
- the usual driving voltage is 3 ⁇ 500V, and the most commonly used is 10 ⁇ 100V.
- the present invention further provides an intermediate buffer device, the structure of which is shown in Figures 3 and 4 below.
- the intermediate buffer has a sealed buffer housing 16 with two ports, an inlet 17 and an outlet 21.
- the intermediate portion may be separated by a diaphragm 20 which is impervious and has considerable flexibility and tensile strength, and the side of the inlet is filled with a liquid 18 which can be pumped by an electroosmotic pump, such as pure water.
- an electroosmotic pump such as pure water.
- the liquid 19 to be pumped is filled, and these liquids are often not pumped by an electroosmotic pump.
- the electroosmotic pump outlet 10 When the electroosmotic pump outlet 10 is connected to the inlet 17 of the intermediate buffer device by a capillary tube 22 and filled with liquid, after the electroosmotic pump is turned on, the fluid pressure generated by the electroosmotic pump is transmitted through the liquid 18 to the intermediate diaphragm 20 of the intermediate buffer device, through The intermediate diaphragm 20 is then transferred to the liquid 19 to be delivered to the other side of the diaphragm, which liquid is forced to flow from the outlet 21 under pressure.
- the electroosmotic pump itself produces only a constant pressure and flow rate, and the actual output is the working fluid having the same pressure and flow rate stored on the outlet side of the buffer device.
- One of the greatest advantages of this method is that the liquid to be pumped does not come into contact with the electroosmotic pump, regardless of the corrosive or destructive effect of the liquid on the electroosmotic pump, which is a problem that cannot be solved by mechanical micropumps.
- an electroosmotic pump can be used to transport a plurality of liquids, and a complicated cleaning process of the electroosmotic pump is not required between the delivery of different liquids, as long as an intermediate buffer device is replaced, and an intermediate buffer device is provided.
- the manufacturing cost is much smaller than that of an electroosmotic pump.
- the anion exchange membrane and the cation exchange membrane are respectively packaged in the channel I and the channel II, the anion and cation exchange membranes are electrically contacted with each other in the pump chamber of the liquid flow output, thereby realizing electric field coupling, and the driving electrode is not present in the output liquid stream.
- the neutralization reaction occurs in the output liquid phase rather than the electrochemical reaction, which fundamentally eliminates the possibility of bubble generation and allows the electroosmotic flow to be output constant.
- the present invention can obtain a large flow electroosmotic flow output with a lower driving voltage, and the voltage used is generally 3 to 500 V, and the commonly used voltage is 10 to 100 V, which is more than usual.
- the voltage used in the osmotic pump is two orders of magnitude lower, which is conducive to the implementation of electrical isolation measures, improved safety and instrument miniaturization.
- the electroosmotic pump of the invention works, its electroosmotic flow rate is proportional to the current passing through, so the electroosmotic flow rate adjustment Convenient, and the electroosmotic flow has no correlation or little correlation with other factors, which is very convenient for the design, manufacture and use of electroosmotic pumps, and is suitable for mass production.
- the electroosmotic pump of the present invention can pump pure water, it can also pump a salt solution and a mixed liquid of water and a polar organic solvent, and can realize a gradient output of the relevant parameters, so the electroosmotic pump of the present invention is easy. Compatible with instruments such as FIA, CE, HPLC and ion chromatography.
- the invention is provided with a radiator member on the outer wall of the passage where the anion and the cation membrane are located, which is beneficial to the removal of the Joule heat and improves the output performance of the pump.
- the present invention which is provided with a buffer tank, is capable of transporting all of the liquid, many of which are normally not transportable by the electroosmotic pump. Furthermore, the present invention enables the pH of the effluent and other components to be controlled by adjusting the composition of the anode and cathode cell solutions. Changing the polarity of the drive voltage changes the direction of the electroosmotic flow.
- Figure 1 shows a schematic diagram of an electroosmotic pump of the present invention
- FIG. 2 is a schematic structural view of an electroosmotic pump according to an embodiment of the present invention.
- Figure 3 is a schematic structural view of an intermediate buffer device
- Figure 4 is a schematic view showing the structure of the present invention with an intermediate buffer device
- Figure 5 is a schematic view of the structure of the present invention which can be used for liquid parameter gradient transport.
- Figure 2 shows an electroosmotic pump with high output pressure and low output flow.
- a Nafion® 117 cation exchange membrane (1) and an NF 201 anion exchange membrane (2) having a width of about lnrai are respectively inserted into the cationic membrane cannula (11) and the anion membrane cannula (12), which are chemically used.
- a stable Teflon tube with a wall thickness of about 0.2 mm and a diameter of about 1 mm. The two ends of the sleeve are sealed with epoxy glue, and the cation exchange membrane (1) and the anion exchange membrane (2) are respectively exposed 10 to 20 mm outside the sleeve.
- the cationic membrane sleeve (11) and the anion membrane sleeve (12) are flattened, they are inserted into the opposite slits at the bottom of the pump chamber (9) and sealed with epoxy glue.
- the end of the sleeve is flush with the inner wall of the pump chamber.
- the cation exchange membrane and the anion exchange membrane exposed to the outside protrude into the pump chamber and are in direct contact with the pump chamber. In order to ensure good electrical contact, it can be in the pump chamber. Fill with an electrolyte such as a 0.1 M Na 2 SO 4 solution.
- the metal bracket (14) In addition to the fixed pump chamber and the ion-membrane sleeve, the metal plate (13) is mainly used for heat dissipation, and the Joule heat generated during the electroosmosis process can be dissipated in a timely and effective manner, so that a large current can be used. It can also be installed on metal brackets and metal plates. The hot film makes the heat dissipation better.
- the temperature of the ion membrane can be controlled within a small variation range, making the electroosmotic flow more stable and reliable.
- the other ends of the cationic membrane sleeve (11) and the anion membrane sleeve (12) are respectively inserted into the positive electrode pool (4) and the negative electrode pool (7), and the positive electrode pool (4) and the negative electrode pool (7) respectively contain positive electrode electrolysis.
- a liquid (5) and a negative electrolyte (8), and a positive electrode column (3) and a negative electrode column (6) are placed, and the positive electrode column (3) and the negative electrode column (6) are respectively connected to a DC power source (not shown) Positive and negative terminals.
- the positive electrode electrolyte (5) and the negative electrode electrolyte (8) are only in contact with the cation exchange membrane (1) and the anion exchange membrane (2) exposed outside the sleeve.
- a certain voltage is applied between the positive electrode (3) and the negative electrode (6), and the cation in the positive electrode electrolyte moves to the pump chamber (9) under the action of the electric field and drags the solvating solvent molecule and the free solvent molecule together.
- the motion forms a positive electroosmotic flow.
- the anion in the negative electrode electrolyte also moves toward the pump chamber (9) under the action of an electric field and drags the solvated solvent molecules and the free solvent molecules together to form a negative electrode electroosmotic flow. Since both the cation exchange membrane and the anion exchange membrane have the ability to prevent the passage of counterions, the positive electroosmotic flow and the negative electroosmotic flow to the pump chamber (9) can only accumulate in the pump chamber (9), such as hydrogen ions and hydroxides. The ions are neutralized into water and, when filled, flow out of the pump chamber outlet (10) to effect pumping of the liquid.
- the electroosmotic pump can generate a current of 0.1 to 10 mA at a driving voltage of 10 to 500 V, and can generate a volume flow rate of about 0.01 to 1 ⁇ ⁇ . If operating at a constant current, a stable electroosmotic flow can be obtained.
- the outlet (10) of the electroosmotic pump of the first embodiment is connected to the inlet (17) of the intermediate buffer device by a capillary tube (22), and the connection ring is used.
- Oxygen seal, capillary (22) should be able to withstand extremely high pressures, such as 50MPa. It is of course also possible to make the intermediate buffer device and the electroosmotic pump in one piece.
- the diaphragm inlet side of the pump chamber (9), capillary (22) and buffer housing (16) are filled with liquid (18) that can be pumped with an electroosmotic pump, such as pure Water, on the other side of the buffer tank (16), is filled with any liquid that needs to be pumped (19).
- an electroosmotic pump such as pure Water
- the electroosmotic pump is turned on, the pressure generated by the pumped pure water is transmitted through the diaphragm to the liquid on the other side of the diaphragm (19), which is pumped under pressure from the outlet (21) of the buffer tank. . Due to the incompressibility of the liquid, the flow rate and pressure of the liquid flowing out of the buffer tank outlet (21) are exactly the same as the liquid flow and pressure pumped by the electroosmotic pump outlet (10).
- the single electroosmotic pump of the present invention can also achieve certain gradient transport, such as pH gradient transport, ionic strength gradient transport, gradient transport of polar small molecule organic solvents, etc., but for pure organic solvents, non-polar organic solvents, large
- the molecular organic solvent cannot be pumped by the electroosmotic pump of the present invention, and gradient transport cannot be achieved.
- binary gradient delivery of any liquid can be achieved.
- the outlet (21a) of the infusion device I and the outlet (21b) of the infusion device II are connected to the two interfaces of the tee (23) by the capillary I (22a) and the capillary II (22b), respectively.
- the seal is sealed and the third port of the tee is connected to the inlet of a mixer (24).
- a mixer 24
- Such a device can achieve a gradient delivery. If the infusion device I delivers pure water, the infusion device II delivers pure acetonitrile, the electroosmotic pump of the infusion device I operates at i!, and the electroosmotic pump of the infusion device II operates at a current of 2 , assuming two The electroosmotic pump in the infusion device uses pumping pure water as the transmission power.
- a mixed liquid in which the ratio of the two contents is continuously changed can be obtained from the mixer outlet (25).
- a change from 5 mA to 0 mA, and a change in f 2 from 0 mA to 5 mA, while ensuring + z mA can achieve a continuous change in acetonitrile from 0% to 100% in the mixed liquid at the mixer outlet (25).
- the flow rate is kept at 0.5 ⁇ .
- multiple such infusion devices can be delivered to a multi-level gradient by means of an infusion set consisting of a multi-pass and a mixer.
Description
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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CN 200410066556 CN1752753B (zh) | 2004-09-22 | 2004-09-22 | 离子膜微流量电渗泵 |
CN200410066556.7 | 2004-09-22 |
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CN105573359A (zh) * | 2015-12-10 | 2016-05-11 | 南京理工大学 | 施加压力控制纳米孔中离子传输选择度的方法 |
CN109529962A (zh) * | 2019-01-18 | 2019-03-29 | 江苏医联生物科技有限公司 | 基于微通道板的薄膜电渗泵及其检测压强与流速的方法 |
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CN2286429Y (zh) * | 1997-03-04 | 1998-07-22 | 中国科学技术大学 | 多孔芯柱电渗泵 |
US6030844A (en) * | 1996-08-16 | 2000-02-29 | Nec Corporation | Method and apparatus for pre-analyzing treatment for subsequent analysis of metal components in volatile alkali solution and non-volatile anion in volatine acid solution and method and apparatus for the subsequent analysis |
CN1372138A (zh) * | 2002-03-29 | 2002-10-02 | 厦门大学 | 一种电泳聚焦浓缩装置 |
CN1410673A (zh) * | 2001-10-09 | 2003-04-16 | 厦门大学 | 微型电渗泵 |
US20030085024A1 (en) * | 2001-09-28 | 2003-05-08 | Santiago Juan G | Control of electrolysis gases in electroosmotic pump systems |
JP2003311275A (ja) * | 2002-04-24 | 2003-11-05 | Kurita Water Ind Ltd | 電気式脱イオン装置 |
Family Cites Families (3)
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EP0727661B1 (en) * | 1995-02-18 | 2003-05-07 | Agilent Technologies Deutschland GmbH | Mixing liquids using electroosmotic flow |
CN1194800C (zh) * | 2001-11-15 | 2005-03-30 | 中国科学院大连化学物理研究所 | 芯片式微流量电渗泵 |
CN1226074C (zh) * | 2002-11-15 | 2005-11-09 | 中国科学院大连化学物理研究所 | 超高压微流量电渗泵 |
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2004
- 2004-09-22 CN CN 200410066556 patent/CN1752753B/zh not_active Expired - Fee Related
- 2004-11-15 WO PCT/CN2004/001294 patent/WO2006032171A1/zh active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH03267753A (ja) * | 1989-12-05 | 1991-11-28 | United Technol Corp <Utc> | イオン性物質検出器 |
CN2114162U (zh) * | 1992-02-13 | 1992-08-26 | 厦门大学 | 阴、阳离子双功能离子色谱抑制柱 |
US6030844A (en) * | 1996-08-16 | 2000-02-29 | Nec Corporation | Method and apparatus for pre-analyzing treatment for subsequent analysis of metal components in volatile alkali solution and non-volatile anion in volatine acid solution and method and apparatus for the subsequent analysis |
CN2286429Y (zh) * | 1997-03-04 | 1998-07-22 | 中国科学技术大学 | 多孔芯柱电渗泵 |
US20030085024A1 (en) * | 2001-09-28 | 2003-05-08 | Santiago Juan G | Control of electrolysis gases in electroosmotic pump systems |
CN1410673A (zh) * | 2001-10-09 | 2003-04-16 | 厦门大学 | 微型电渗泵 |
CN1372138A (zh) * | 2002-03-29 | 2002-10-02 | 厦门大学 | 一种电泳聚焦浓缩装置 |
JP2003311275A (ja) * | 2002-04-24 | 2003-11-05 | Kurita Water Ind Ltd | 電気式脱イオン装置 |
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CN1752753A (zh) | 2006-03-29 |
CN1752753B (zh) | 2010-04-28 |
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