WO2006027865A3 - Method of transforming chloroplast of composite plant - Google Patents

Method of transforming chloroplast of composite plant Download PDF

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Publication number
WO2006027865A3
WO2006027865A3 PCT/JP2005/004899 JP2005004899W WO2006027865A3 WO 2006027865 A3 WO2006027865 A3 WO 2006027865A3 JP 2005004899 W JP2005004899 W JP 2005004899W WO 2006027865 A3 WO2006027865 A3 WO 2006027865A3
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WO
WIPO (PCT)
Prior art keywords
composite plant
chloroplast
protein
transforming
terminator
Prior art date
Application number
PCT/JP2005/004899
Other languages
French (fr)
Other versions
WO2006027865A2 (en
Inventor
Ken-Ichi Tomizawa
Hirosuke Kanamoto
Akiho Yokota
Hiroshi Asao
Original Assignee
Res Inst Innovative Tech Earth
Science And Technology Nat Uni
Ken-Ichi Tomizawa
Hirosuke Kanamoto
Akiho Yokota
Hiroshi Asao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Inst Innovative Tech Earth, Science And Technology Nat Uni, Ken-Ichi Tomizawa, Hirosuke Kanamoto, Akiho Yokota, Hiroshi Asao filed Critical Res Inst Innovative Tech Earth
Publication of WO2006027865A2 publication Critical patent/WO2006027865A2/en
Publication of WO2006027865A3 publication Critical patent/WO2006027865A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An object of present invention is to provide a transformation method which can effectively express a useful protein such as a medical protein in a composite plant chloroplast without producing a harmful secondary metabolite. A vector having a promoter of RNA operon derived from a composite plant chloroplast genome and a terminator of a psbA gene between a ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene and an acetyl CoA carboxylase subunit gene, and having a nucleotide sequence encoding an expression protein between the promoter and the terminator is introduced into a chloroplast of a composite plant.
PCT/JP2005/004899 2004-09-09 2005-03-14 Method of transforming chloroplast of composite plant WO2006027865A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004262632A JP4740567B2 (en) 2004-09-09 2004-09-09 A method for transformation of chloroplasts of Asteraceae plants
JP2004-262632 2004-09-09

Publications (2)

Publication Number Publication Date
WO2006027865A2 WO2006027865A2 (en) 2006-03-16
WO2006027865A3 true WO2006027865A3 (en) 2006-06-08

Family

ID=35541586

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2005/004899 WO2006027865A2 (en) 2004-09-09 2005-03-14 Method of transforming chloroplast of composite plant

Country Status (2)

Country Link
JP (1) JP4740567B2 (en)
WO (1) WO2006027865A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008121947A1 (en) * 2007-03-30 2008-10-09 University Of Central Florida Research Foundation, Inc. Chloroplasts engineered to express pharmaceutical proteins in edible plants
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
JP2009207398A (en) * 2008-03-03 2009-09-17 Nara Institute Of Science & Technology Vector for transforming chloroplast of compositae family plant, human thioredoxin-producing transformed plant and production method thereof as well as composition containing human thioredoxin
US9724400B2 (en) 2009-11-09 2017-08-08 The Trustees Of The University Of Pennsylvania Administration of plant expressed oral tolerance agents
US10865419B2 (en) 2011-10-24 2020-12-15 The Trustees Of The University Of Pennsylvania Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes
CN113061567A (en) * 2021-04-06 2021-07-02 石河子大学 Method for extracting chloroplast of kochia scoparia

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08298887A (en) * 1995-05-08 1996-11-19 Yamashita Yukinori Tissue culture of santalum and its secondary proliferation
EP1245149A1 (en) * 2001-03-29 2002-10-02 greenovation Biotech GmbH Plastid transformation of lycopersicon plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05244971A (en) * 1992-03-06 1993-09-24 Nippon Oil Co Ltd Production of compound of taxanes
JPH0731310A (en) * 1993-07-20 1995-02-03 Asahi Denka Kogyo Kk Production method of seedling of curculigo plant
JPH09107832A (en) * 1995-10-17 1997-04-28 Sapporo Breweries Ltd Method for proliferating clone of paphiopedilum
JPH11313680A (en) * 1998-04-30 1999-11-16 Iwate Prefecture Cecropin gene
JP2001078602A (en) * 1999-09-10 2001-03-27 Central Res Inst Of Electric Power Ind Method for increasing amount of growth of plant

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08298887A (en) * 1995-05-08 1996-11-19 Yamashita Yukinori Tissue culture of santalum and its secondary proliferation
EP1245149A1 (en) * 2001-03-29 2002-10-02 greenovation Biotech GmbH Plastid transformation of lycopersicon plants

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ABDULLAH A A ET AL: "MICROPROPAGATION OF MATURE CALABRIAN PINE PINUS-BRUTIA TEN. FROM FASCICULAR BUDS", TREE PHYSIOLOGY, vol. 3, no. 2, 1987, pages 123 - 136, XP008062117, ISSN: 0829-318X *
AHMED M F ET AL: "Factors affecting adventitious shoot and root formation from the avocado cultivar 'Hass' (Persea americana, Mill.) in vitro", GARTENBAUWISSENSCHAFT, vol. 66, no. 6, November 2001 (2001-11-01), pages 293 - 297, XP008062204, ISSN: 0016-478X *
BROWN C ET AL: "PLANT REGENERATION FROM PROTOPLASTS OF A WILD LETTUCE SPECIES LACTUCA-SALIGNA L", PLANT CELL REPORTS, vol. 6, no. 3, 1987, pages 180 - 182, XP008062115, ISSN: 0721-7714 *
HAGIO TAKASHI: "Adventitious shoot regeneration from immature embryos of sorghum", PLANT CELL TISSUE AND ORGAN CULTURE, vol. 68, no. 1, January 2002 (2002-01-01), pages 65 - 72, XP008062111, ISSN: 0167-6857 *
HEIFETZ P B: "Genetic engeneering of the chloroplast", BIOCHIMIE, MASSON, PARIS, FR, vol. 82, 2000, pages 655 - 666, XP002190805, ISSN: 0300-9084 *
SIDOROV V A ET AL: "STABLE CHLOROPLAST TRANSFORMATION IN POTATO: USE OF GREEN FLUORESCENT PROTEIN AS A PLASTID MARKER", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 19, no. 2, July 1999 (1999-07-01), pages 209 - 216, XP000886694, ISSN: 0960-7412 *
ZORA SVAB ET AL: "High-frequence plastid transformation in tobacco by selection for a chimeric aadA gene", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 90, 1993, pages 913 - 917, XP002190803, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2006027865A2 (en) 2006-03-16
JP2006075078A (en) 2006-03-23
JP4740567B2 (en) 2011-08-03

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