WO2006018643A3 - Electrochemical sensors - Google Patents

Electrochemical sensors Download PDF

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Publication number
WO2006018643A3
WO2006018643A3 PCT/GB2005/003229 GB2005003229W WO2006018643A3 WO 2006018643 A3 WO2006018643 A3 WO 2006018643A3 GB 2005003229 W GB2005003229 W GB 2005003229W WO 2006018643 A3 WO2006018643 A3 WO 2006018643A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
edot
conjugate
binding
electrode
Prior art date
Application number
PCT/GB2005/003229
Other languages
French (fr)
Other versions
WO2006018643A2 (en
Inventor
Simon J Higgins
Fouzi Mouffouk
Original Assignee
Univ Liverpool
Simon J Higgins
Fouzi Mouffouk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Liverpool, Simon J Higgins, Fouzi Mouffouk filed Critical Univ Liverpool
Publication of WO2006018643A2 publication Critical patent/WO2006018643A2/en
Publication of WO2006018643A3 publication Critical patent/WO2006018643A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

The present invention relates to an electrochemical sensor comprising at least one conjugate having a ligand attached to 3,4-ethylenedioxythiophene (EDOT) or derivative or polymer thereof by means of a spacing element, said ligand being capable of binding a target molecule in a sample, and said EDOT being attached to an electrode such that the binding of a target molecule to the ligand results in a detectable change in the electrochemical properties of the conjugate on the electrode. The sensor is particularly useful for testing biological and chemical material within samples.
PCT/GB2005/003229 2004-08-17 2005-08-17 Electrochemical sensors WO2006018643A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0418327A GB0418327D0 (en) 2004-08-17 2004-08-17 Electrochemical sensors
GB0418327.3 2004-08-17

Publications (2)

Publication Number Publication Date
WO2006018643A2 WO2006018643A2 (en) 2006-02-23
WO2006018643A3 true WO2006018643A3 (en) 2007-11-15

Family

ID=33042187

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/003229 WO2006018643A2 (en) 2004-08-17 2005-08-17 Electrochemical sensors

Country Status (2)

Country Link
GB (1) GB0418327D0 (en)
WO (1) WO2006018643A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008039151A1 (en) * 2006-09-26 2008-04-03 Agency For Science, Technology And Research Electrically conducting polymers
US20100126880A1 (en) * 2006-11-10 2010-05-27 Yu Hsiao-Hua Dna complexing agents
US7708908B2 (en) * 2007-02-28 2010-05-04 The Regents Of The University Of Michigan Carboxylic acid-modified EDOT for bioconjugation
WO2008130327A1 (en) * 2007-04-20 2008-10-30 Agency For Science, Technology And Research Biointerfaces for biomolecule detection
WO2008130326A1 (en) * 2007-04-20 2008-10-30 Agency For Science, Technology And Research Polymeric films

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ANALYTICA CHIMICA ACTA, vol. 496, no. 1-2, 31 October 2003 (2003-10-31), pages 81 - 91, ISSN: 0003-2670 *
BAE AH-HYUN ET AL: "Electrochemical fabrication of single-walled carbon nanotubes-DNA complexes by poly(ethylenedioxythiophene) and photocurrent generation by excitation of an intercalated chromophore.", ORGANIC & BIOMOLECULAR CHEMISTRY. 21 APR 2004, vol. 2, no. 8, 21 April 2004 (2004-04-21), pages 1139 - 1144, XP002361432, ISSN: 1477-0520 *
BIOSENSORS & BIOELECTRONICS, vol. 18, no. 11, 1 October 2003 (2003-10-01), pages 1329 - 1337, ISSN: 0956-5663 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1 October 2003 (2003-10-01), MAKOWER ALEXANDER ET AL: "New principle of direct real-time monitoring of the interaction of cholinesterase and its inhibitors by piezolectric biosensor.", XP002382465, Database accession no. PREV200400061330 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 31 October 2003 (2003-10-31), TAFT BRADFORD J ET AL: "Engineering DNA-electrode connectivities: Manipulation of linker length and structure.", XP002382466, Database accession no. PREV200400107792 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; March 2001 (2001-03-01), MOUSTY CHRISTINE ET AL: "Electrogeneration of a hydrophilic cross-linked polypyrrole film for enzyme electrode fabrication. Application to the amperometric detection of glucose", XP002382464, Database accession no. PREV200100203797 *
ELECTROANALYSIS, vol. 13, no. 3, March 2001 (2001-03-01), pages 186 - 190, ISSN: 1040-0397 *
KROS ET AL.: "POLY(3,4-ETHYLENEDIOXYTHIOPHENE)-BASED COPOLYMERS FOR BIOSENSOR APPLICATIONS", JOURNAL OF POLYMER SCIENCE, vol. 40, 2001, pages 738 - 747, XP002361455 *
KROS ET AL.: "Poly(3,4-ethylenedioxythiophene)-Based Glucose Biosensors", ADVANCED MATERIALS, vol. 13, no. 20, 2001, XP002361431 *
TRIPPÉ GAËLLE ET AL: "Crown-tetrathiafulvalenes attached to a pyrrole or an EDOT unit: synthesis, electropolymerization and recognition properties.", CHEMISTRY (WEINHEIM AN DER BERGSTRASSE, GERMANY) 3 DEC 2004, vol. 10, no. 24, 3 December 2004 (2004-12-03), pages 6497 - 6509, XP002361430, ISSN: 0947-6539 *

Also Published As

Publication number Publication date
GB0418327D0 (en) 2004-09-22
WO2006018643A2 (en) 2006-02-23

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