WO2006004768A3 - Immortal cell lines containing non-mitochondrial genetic material within the mitochondria - Google Patents

Immortal cell lines containing non-mitochondrial genetic material within the mitochondria Download PDF

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Publication number
WO2006004768A3
WO2006004768A3 PCT/US2005/022958 US2005022958W WO2006004768A3 WO 2006004768 A3 WO2006004768 A3 WO 2006004768A3 US 2005022958 W US2005022958 W US 2005022958W WO 2006004768 A3 WO2006004768 A3 WO 2006004768A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell lines
mitochondria
mitochondrial
lines containing
genetic material
Prior art date
Application number
PCT/US2005/022958
Other languages
French (fr)
Other versions
WO2006004768A2 (en
Inventor
Sung Sup Park
Original Assignee
Paik Medicine International In
Sung Sup Park
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paik Medicine International In, Sung Sup Park filed Critical Paik Medicine International In
Publication of WO2006004768A2 publication Critical patent/WO2006004768A2/en
Publication of WO2006004768A3 publication Critical patent/WO2006004768A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A new cell line has been established by previous methods of immortalization with EBV that contains the HCV RNA genome staff y within the mitochondria. Thus, a method for the introduction of non-mitochondrial material into mitochondrial, immortal cell lines, and methods of using the cell lines to produce mitochondrial proteins are presented. The methods are particularly useful for the production of cell lines containing viral genomes that have a part or all of their life cycle within the mitochondria.
PCT/US2005/022958 2004-06-28 2005-06-28 Immortal cell lines containing non-mitochondrial genetic material within the mitochondria WO2006004768A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58394504P 2004-06-28 2004-06-28
US60/583,945 2004-06-28

Publications (2)

Publication Number Publication Date
WO2006004768A2 WO2006004768A2 (en) 2006-01-12
WO2006004768A3 true WO2006004768A3 (en) 2006-08-24

Family

ID=35783321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/022958 WO2006004768A2 (en) 2004-06-28 2005-06-28 Immortal cell lines containing non-mitochondrial genetic material within the mitochondria

Country Status (1)

Country Link
WO (1) WO2006004768A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006023956A2 (en) * 2004-08-23 2006-03-02 Paik Medicine International In Method for gene transfer into the organelles of cells: direct gene transfer to mitochondria

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100068A (en) * 1997-01-21 2000-08-08 Paik-Inje Memorial Institute For Biomedical Science Method of protein production using mitochondrial translation system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100068A (en) * 1997-01-21 2000-08-08 Paik-Inje Memorial Institute For Biomedical Science Method of protein production using mitochondrial translation system

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
IKEDA ET AL.: "Analysis of the cell tropism of HCV by using in vitro HCV-infected human lymphocytes and hepatocytes", J. HEPATOL., vol. 27, no. 3, 8 August 2000 (2000-08-08), pages 445 - 454, XP001033876 *
SUNG ET AL.: "Establishment of B-Cell Lymphoma Cell Lines Persistently Infected with Hepatitis C Virus In Vivo and In Vitro: the Apoptotic Effects of Virus Infection", J. VIROL., vol. 77, no. 3, February 2003 (2003-02-01), pages 2134 - 2146, XP003000083 *

Also Published As

Publication number Publication date
WO2006004768A2 (en) 2006-01-12

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