WO2006003638A3 - Novel method for labeling nucleic acid in a sequence-specific manner, and method for detecting nucleic acid using the same - Google Patents

Novel method for labeling nucleic acid in a sequence-specific manner, and method for detecting nucleic acid using the same Download PDF

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Publication number
WO2006003638A3
WO2006003638A3 PCT/IB2005/052245 IB2005052245W WO2006003638A3 WO 2006003638 A3 WO2006003638 A3 WO 2006003638A3 IB 2005052245 W IB2005052245 W IB 2005052245W WO 2006003638 A3 WO2006003638 A3 WO 2006003638A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
labeled
sequence
same
specific manner
Prior art date
Application number
PCT/IB2005/052245
Other languages
French (fr)
Other versions
WO2006003638A2 (en
Inventor
Ichiro Mitsuhara
Yuko Ohashi
Original Assignee
Nat Inst Of Agrobio Sciences
Ichiro Mitsuhara
Yuko Ohashi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Inst Of Agrobio Sciences, Ichiro Mitsuhara, Yuko Ohashi filed Critical Nat Inst Of Agrobio Sciences
Priority to JP2007519959A priority Critical patent/JP4729677B2/en
Publication of WO2006003638A2 publication Critical patent/WO2006003638A2/en
Publication of WO2006003638A3 publication Critical patent/WO2006003638A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Abstract

The present invention provides a method for producing a nucleic acid having an introduced label, comprising the steps of: A) providing a nucleic acid to be labeled and a template nucleic acid, the template nucleic acid being complementary to at least a portion of the nucleic acid to be labeled; B) hybridizing the nucleic acid to be labeled with the template nucleic acid to produce a nucleic acid to be labeled-template nucleic acid complex; C) subjecting the nucleic acid to be labeled-template nucleic acid complex to an extension reaction, inwhich a nucleic acid synthesizing enzyme and labeled nucleotides are used, so that at least one of the labeled nucleotides is incorporated into the nucleic acid to be labeled based on the template nucleic acid; and D) recovering the elongated nucleic acid to be labeled from the resultant mixture, after the extension reaction.
PCT/IB2005/052245 2004-07-07 2005-07-06 Novel method for labeling nucleic acid in a sequence-specific manner, and method for detecting nucleic acid using the same WO2006003638A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007519959A JP4729677B2 (en) 2004-07-07 2005-07-06 Method for labeling nucleic acid in sequence-specific manner, and novel nucleic acid detection method using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004-201212 2004-07-07
JP2004201212A JP2006020560A (en) 2004-07-07 2004-07-07 Method for sequence-specifically labeling nucleic acid, and new nucleic acid detection method using the same

Publications (2)

Publication Number Publication Date
WO2006003638A2 WO2006003638A2 (en) 2006-01-12
WO2006003638A3 true WO2006003638A3 (en) 2006-03-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/052245 WO2006003638A2 (en) 2004-07-07 2005-07-06 Novel method for labeling nucleic acid in a sequence-specific manner, and method for detecting nucleic acid using the same

Country Status (2)

Country Link
JP (3) JP2006020560A (en)
WO (1) WO2006003638A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5697059B2 (en) * 2013-06-25 2015-04-08 古河電気工業株式会社 Target substance detection method and detection apparatus set using immunochromatography

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571669A (en) * 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
US20040146867A1 (en) * 2003-01-24 2004-07-29 Slattum Paul M Compounds and processes for single-pot attachment of a label to siRNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571669A (en) * 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
US20040146867A1 (en) * 2003-01-24 2004-07-29 Slattum Paul M Compounds and processes for single-pot attachment of a label to siRNA

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BEHLKE M A ET AL: "Use of high specific activity StarFireTM oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe", BIOTECHNIQUES, vol. 29, no. 4, October 2000 (2000-10-01), pages 892 - 897, XP001208234, ISSN: 0736-6205 *
DAUDE NATHALIE ET AL: "Specific inhibition of pathological prion protein accumulation by small interfering RNAs.", JOURNAL OF CELL SCIENCE, vol. 116, no. 13, 1 July 2003 (2003-07-01), pages 2775 - 2779, XP002362880, ISSN: 0021-9533 *
GANDRILLON O ET AL: "A rapid and convenient method to prepare DIG-labelled RNA probes for use in non-radioactive in situ hybridization.", MOLECULAR AND CELLULAR PROBES. FEB 1996, vol. 10, no. 1, February 1996 (1996-02-01), pages 51 - 55, XP002362879, ISSN: 0890-8508 *
SANGER F ET AL: "DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, NEW YORK, NY, US, vol. 74, no. 12, 1 December 1977 (1977-12-01), pages 5463 - 5467, XP000603873 *

Also Published As

Publication number Publication date
JP2006020560A (en) 2006-01-26
JP5218944B2 (en) 2013-06-26
WO2006003638A2 (en) 2006-01-12
JP2011062207A (en) 2011-03-31
JP4729677B2 (en) 2011-07-20
JP2008505635A (en) 2008-02-28

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