WO2006002141A3 - Detection of post-translationally modified peptides with liquid crystals - Google Patents

Detection of post-translationally modified peptides with liquid crystals Download PDF

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Publication number
WO2006002141A3
WO2006002141A3 PCT/US2005/021887 US2005021887W WO2006002141A3 WO 2006002141 A3 WO2006002141 A3 WO 2006002141A3 US 2005021887 W US2005021887 W US 2005021887W WO 2006002141 A3 WO2006002141 A3 WO 2006002141A3
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WO
WIPO (PCT)
Prior art keywords
post
translationally modified
sample
detection
liquid crystals
Prior art date
Application number
PCT/US2005/021887
Other languages
French (fr)
Other versions
WO2006002141A2 (en
Inventor
Nicholas L Abbott
Brian H Clare
Paul J Bertics
Original Assignee
Wisconsin Alumni Res Found
Nicholas L Abbott
Brian H Clare
Paul J Bertics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/711,517 external-priority patent/US8133680B2/en
Application filed by Wisconsin Alumni Res Found, Nicholas L Abbott, Brian H Clare, Paul J Bertics filed Critical Wisconsin Alumni Res Found
Publication of WO2006002141A2 publication Critical patent/WO2006002141A2/en
Publication of WO2006002141A3 publication Critical patent/WO2006002141A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Manufacturing & Machinery (AREA)
  • Inorganic Chemistry (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method for differentiating between a post-translationally modified peptide and a peptide contained in a sample, comprising:(a) contacting the sample with a peptide attachment surface to create a peptidized surface, wherein the sample includes at least one functional group; (b) contacting the peptidized surface with a recognition reagent that selectively binds or forms a complex with the post-translationally modified peptide in the sample to provide an incubated surface; and (c) contacting a liquid crystal with the incubated surface and detecting presence of post-translationally modified peptide in the sample with the liquid crystal.
PCT/US2005/021887 2004-06-18 2005-06-20 Detection of post-translationally modified peptides with liquid crystals WO2006002141A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58119804P 2004-06-18 2004-06-18
US60/581,198 2004-06-18
US10/711,517 2004-09-23
US10/711,517 US8133680B2 (en) 2003-09-23 2004-09-23 Using liquid crystals to detect affinity microcontact printed biomolecules

Publications (2)

Publication Number Publication Date
WO2006002141A2 WO2006002141A2 (en) 2006-01-05
WO2006002141A3 true WO2006002141A3 (en) 2006-04-06

Family

ID=35006327

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/021887 WO2006002141A2 (en) 2004-06-18 2005-06-20 Detection of post-translationally modified peptides with liquid crystals

Country Status (1)

Country Link
WO (1) WO2006002141A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007147002A2 (en) * 2006-06-13 2007-12-21 Wisconsin Alumni Research Foundation Beta-peptide lyotropic liquid crystals and methods of manufacture and use thereof
WO2008045311A2 (en) 2006-10-06 2008-04-17 Qualcomm Mems Technologies, Inc. Illumination device with built-in light coupler
DE102008019928A1 (en) * 2008-04-21 2009-12-31 Siemens Healthcare Diagnostics Gmbh Procedures for applying spots with capture molecules on substrate surface for chip, particularly optical sensor, involve washing substrate surface of chip by dipping in aqueous solution of cationic polyelectrolyte
IT1400874B1 (en) * 2010-07-05 2013-07-02 Univ Degli Studi Genova SUPPORT FLOOR WITH A ULTRA-FLAT SURFACE AND DEVICE FOR THE DETECTION OF ANTIGEN INCLUDING THE PLANAR SUPPORT.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063329A1 (en) * 1998-06-05 1999-12-09 The Regents Of The University Of California Optical amplification of molecular interactions using liquid crystals
EP0965835A2 (en) * 1998-05-19 1999-12-22 Hitachi, Ltd. Sensor and measuring apparatus using the same
US20030017508A1 (en) * 2000-06-05 2003-01-23 Chiron Corporation Microarrays on mirrored substrates for performing proteomic analyses
US20040023366A1 (en) * 2000-06-02 2004-02-05 Stefan Dickopf Surface for the immobilization of ligands

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0965835A2 (en) * 1998-05-19 1999-12-22 Hitachi, Ltd. Sensor and measuring apparatus using the same
WO1999063329A1 (en) * 1998-06-05 1999-12-09 The Regents Of The University Of California Optical amplification of molecular interactions using liquid crystals
US20040023366A1 (en) * 2000-06-02 2004-02-05 Stefan Dickopf Surface for the immobilization of ligands
US20030017508A1 (en) * 2000-06-05 2003-01-23 Chiron Corporation Microarrays on mirrored substrates for performing proteomic analyses

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HARNETT C K ET AL: "Bioactive templates fabricated by low-energy electron beam lithography of self-assembled monolayers", LANGMUIR; 2001 ACS, WASHINGTON, DC, USA, vol. 17, no. 1, 12 July 2000 (2000-07-12), pages 178 - 182, XP002349465 *
IVANOV STANIMIR S ET AL: "Antibodies immobilized as arrays to profile protein post-translational modifications in mammalian cells", MOLECULAR & CELLULAR PROTEOMICS, vol. 3, no. 8, 3 May 2004 (2004-05-03), pages 788 - 795, XP002349523, ISSN: 1535-9476 *
LOPEZ GABRIEL P ET AL: "Fabrication and imaging of two-dimensional patterns of proteins adsorbed on self-assembled monolayers by scanning electron microscopy", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 115, no. 23, 17 November 1993 (1993-11-17), pages 10774 - 10781, XP002349401, ISSN: 0002-7863 *
MRKSICH ET AL: "Using self-assembled momolayers to understand the interactions of man-made surfaces with proteins and cells", ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, ANNUAL REVIEWS INC., PALO ALTO, CA, US, vol. 25, 1996, pages 55 - 78, XP002103615, ISSN: 1056-8700 *
SIGAL G B ET AL: "SELF-ASSEMBLED MONOLAYER FOR THE BINDING AND STUDY OF HISTIDINE-TAGGED PROTEINS BY SURFACE PLASMON RESONANCE", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 68, no. 3, 1 February 1996 (1996-02-01), pages 490 - 497, XP000916112, ISSN: 0003-2700 *
WANG MEIJIA ET AL: "Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody-antigen reactions.", BIOSENSORS & BIOELECTRONICS, vol. 19, no. 6, 15 January 2004 (2004-01-15), pages 575 - 582, XP002349400, ISSN: 0956-5663 *

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Publication number Publication date
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