WO2005124351A8 - A sensitive antibody-based method for detecting cryptosporidium parvum oocysts in water - Google Patents
A sensitive antibody-based method for detecting cryptosporidium parvum oocysts in waterInfo
- Publication number
- WO2005124351A8 WO2005124351A8 PCT/US2005/018936 US2005018936W WO2005124351A8 WO 2005124351 A8 WO2005124351 A8 WO 2005124351A8 US 2005018936 W US2005018936 W US 2005018936W WO 2005124351 A8 WO2005124351 A8 WO 2005124351A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- parvum
- cpv
- oocysts
- hominis
- oocyst
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
A viral symbiont (CPV) of Cryptosporidium parvum and Cryptosporidium hominis sporozoites has been characterized and a CPV capsid protein, CPV40, has been identified as a target for sensitive detection of C. parvum. Recombinant CPV40 was produced in E. coli, purified by affinity chromatography, and used to prepare polyclonal rabbit sera specific for the viral capsid protein. Anti-rCPV40 recognized a 40 kDa and a 30 kDa protein in C. parvum oocysts and appeared to localize to the apical end of the parasite. Anti-rCPV40 serum was capable of detecting as few as one C. parvum or C. hominis oocyst in a dot blot assay, the sensitivity being at least 1000-fold greater than sera reactive with total native C. parvum protein or specific for the 41 kDa C. parvum oocyst surface antigen. Water samples were seeded with C parvum oocysts and incubated at 4, 20, or 25 °C for greater than 3 months to determine if CPV levels were correlated with oocyst infectivity and viral particle presence. While sporozoite infectivity declined by more than 75% after 1 month at 25 °C, the CPV signal was similar to that of control samples at 4 °C. By three months at 20 °C, the C. parvum oocysts were found to be non-infectious, but retained a high CPV signal. CPV is an excellent target for sensitive detection of C. parvum and C. hominis oocysts in water, but may persist for an indefinite time after oocysts become non-infectious.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86393904A | 2004-06-09 | 2004-06-09 | |
| US10/863,939 | 2004-06-09 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2005124351A2 WO2005124351A2 (en) | 2005-12-29 |
| WO2005124351A3 WO2005124351A3 (en) | 2006-04-13 |
| WO2005124351A8 true WO2005124351A8 (en) | 2006-12-21 |
Family
ID=35510371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2005/018936 WO2005124351A2 (en) | 2004-06-09 | 2005-05-31 | A sensitive antibody-based method for detecting cryptosporidium parvum oocysts in water |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2005124351A2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183653A (en) * | 2011-03-21 | 2011-09-14 | 吉林大学 | Cryptosporidium parvum immune colloidal gold detection test paper strip and production method thereof |
| CN102221619A (en) * | 2011-06-07 | 2011-10-19 | 吉林大学 | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection method and kit of cryptosporidium virus capsid protein antibody |
| CN110967241B (en) * | 2019-12-13 | 2022-07-29 | 扬州大学 | Dyeing method of Eimeria tenella protoplast |
-
2005
- 2005-05-31 WO PCT/US2005/018936 patent/WO2005124351A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005124351A2 (en) | 2005-12-29 |
| WO2005124351A3 (en) | 2006-04-13 |
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