WO2005110366A1

WO2005110366A1 - An estradiol derivative or an estratopone derivative containing sustained release intraocular implant as well as related methods of manufacturing

Info

Publication number: WO2005110366A1
Application number: PCT/US2005/014257
Authority: WO
Inventors: Jane Guo Shiah; Paul W. Dickinson
Original assignee: Allergan, Inc.
Priority date: 2004-04-30
Filing date: 2005-04-21
Publication date: 2005-11-24
Also published as: BRPI0510471A; US20050244471A1; CA2564948A1; AU2005244216A1; EP1748757A1; JP2007535367A

Abstract

Biocompatible intraocular implants include an anti-angiogenic agent, such as estradiol derivative or an estratopone and a biodegradable polymer that is effective to facilitate release of the anti-angiogenic agent into an eye for an extended period of time. The therapeutic agents of the implants may be associated with a biodegradable polymer matrix, such as a matrix that is substantially free of a polyvinyl alcohol. The implants may be placed in an eye to treat or reduce the occurrence of one or more ocular conditions, such as angiogenisis, ocular tumors, and the like.

Description

AN ESTRADIOL DERIVATIVE OR AN ESTRATOPONE DERIVATIVE CONTAINING SUSTAINED RELEASE INTRAOCULAR IMPLANT AS WELL AS RELATED METHODS OF MANUFACTURING

10 BACKGROUND

The present invention generally relates to devices and methods to treat an eye of a patient, and more specifically to intraocular implants that provide extended release of a therapeutic agent to an eye in which 15 the implant is placed, and to methods of making and using such implants, for example, to treat or reduce neovascularization, angiogenesis, tumor growth, and the like.

Angiogenesis, the process of vascularization, has been implicated 20 in a host of biological disorders including cancer, macular degeneration and arthritis. Spawned by the therapeutic potential associated with the inhibition of pathological angiogenesis, a flurry of activity has led to the discovery of a variety of antiangiogenic compounds which exhibit clinical utility. The discovery of 2-methoxyestradiol (2ME or 2ME2) by Folkman 25 et al has demonstrated evidence for potent antiangiogenic activity by the estrane steroid family and has provided the most potent endogenous mammalian inhibitor of tubulin polymerization yet discovered. (See U.S. Pat. No. 5,504,074.) Additionally, Fotsis et al have shown that of 2- methoxyestradiol exhibits in vitro anti-mitotic properties and reversible 30 inhibition of cell proliferation while confluent cultures are unaffected. (See Fotsis, et. al. Nature 1994, 368, 237.) Preclinical and clinical trials have also shown 2-methoxyestradiol to be promising in the treatment of several angiogenic disorders. 2-Methoxyestradiol is the metabolite of endogenous estradiol in mammalian systems. It demonstrates several biological activities in the inhibition of cell growth. Apoptosis of endothelial cells by 2- Methoxyestradiol leads to inhibition of angiogenesis. Particularly, 2- Methoxyestradiol inhibits vascular endothelial growth factor (VEGF)- induced corneal neovascularization. In addition, 2-Methoxyestradiol has been reported to exhibit antiangiogenic activity through the inhibition of tubulin polymerization by binding at the colchicine binding site. In contrast to 2-methoxyestradiol, colchicine exhibits minimal selectivity, is highly cytotoxic and as a result, its clinical use has been limited due to this low therapeutic index. Since the discovery of 2-methoxyestradiol, structure-activity relationship studies have yielded several 2-substituted estradiol derivatives that exhibit greater affinity for the colchicine binding site, as well as displaying greater cytotoxic responses in cancer cell lines. While the full clinical potential of 2-methoxyestradiol and these related compounds continues to be investigated, little remains known about the relationship between the observed antiangiogenic activity of 2- methoxyestradiol and its ability to bind to tubulin 2-methoxyestradiol is orally active in a wide range of tumor models, and inhibits tumor growth at substantially non-toxic doses. The ability of 2-methoxy estradiol to inhibit metastatic spread adds to its therapeutic value for cancer treatment at various stages of the disease. (Pribluda et al., "2-Methoxyestradiol: An endogenous antiangiogenic and antiproliferative drug candidate", Cancer and Metastasis Reviews, 19: 173-179 (2000)).

Anti-tumor effects of 2-methoxyestradiol are discussed by Schumacher et al., "The physiological estrogen metabolite 2- methoxyestradiol reduces tumor growth and induces apoptosis in human solid tumors", J Cancer Res Clin Oncol, 127:405-410 (2001 ). Miller et al. ("Synthesis and Structure-Activity Profiles of A- Homoestranes, the Estratopones", J. Med. Chem. 40:3836-3841 (1997)) discuss a number of cholchicine/2-methoxyestradiol hybrids. These hybrids possess an A-ring tropone system with keto functionality at either the C-2, C-3, or C-4 position of the steroid nucleus. Most of the hybrids inhibited polymerization of tubulin.

U.S. Patent No. 6,713,081 discloses ocular implant devices made from polyvinyl alcohol and used for the delivery of a therapeutic agent to an eye in a controlled and sustained manner. The implants may be placed subconjunctivally or intravitreally in an eye.

Biocompatible implants for placement in the eye have been disclosed in a number of patents, such as U.S. Pat. Nos. 4,521,210; 4,853,224; 4,997,652; 5, 164, 188; 5,443,505; 5,501 ,856; 5,766,242;

5,824,072; 5,869,079; 6,074,661 ; 6,331 ,313; 6,369,116; and 6,699,493.

It would be advantageous to provide eye implantable drug delivery systems, such as intraocular implants, and methods of using such systems, that are capable of releasing a therapeutic agent at a sustained or controlled rate for extended periods of time and in amounts with few or no negative side effects.

SUMMARY

The present invention provides new drug delivery systems, and methods of making and using such systems, for extended or sustained drug release into an eye, for example, to achieve one or more desired therapeutic effects. The drug delivery systems are in the form of implants or implant elements that may be placed in an eye. The present systems and methods advantageously provide for extended release times of one or more therapeutic agents. Thus, the patient in whose eye the implant has been placed receives a therapeutic amount of an agent for a long or extended time period without requiring additional administrations of the agent. For example, the patient has a substantially consistent level of therapeutically active agent available for consistent treatment of the eye over a relatively long period of time, for example, on the order of at least about one week, such as between about two and about six months after receiving an implant. Such extended release times facilitate obtaining successful treatment results. Intraocular implants in accordance with the disclosure herein comprise a therapeutic component and a drug release sustaining component associated with the therapeutic component. In accordance with the present invention, the therapeutic component comprises, consists essentially of, or consists of, an antiangiogenic compound or compounds. For example, the therapeutic component may comprise, consist essentially of, or consist of, an estradiol derivative, an estratopone, or a combination thereof. Or, the therapeutic component may comprise, consist essentially of, or consist of, anacortate. The drug release sustaining component is associated with the therapeutic component to sustain release of an amount of the anti-angiogenic compound, such as, an estradiol derivative and/or an estratopone into an eye in which the implant is placed. The amount of the antiangiogenic compound is released into the eye for a period of time greater than about one week after the implant is placed in the eye and is effective in reducing or treating ocular conditions, such as neovascularization, angiogenesis, tumor growth, and the like.

In one embodiment, the intraocular implants comprise an estradiol derivative and a biodegradable polymer matrix that is substantially free of polyvinyl alcohol. The estradiol derivative is associated with a biodegradable polymer matrix that degrades at a rate effective to sustain release of an amount of the estradiol derivative from the implant effective to treat an ocular condition. The intraocular implant is biodegradable or bioerodible and provides a sustained release of the estradiol derivative in an eye for extended periods of time, such as for more than one week, for example for about three months or more and up to about six months or more. In certain implants, the estradiol derivative is 2-methoxyestradiol.

In another embodiment, the intraocular implants comprise an estratopone and a biodegradable polymer matrix. The estratopone is associated with a biodegradable polymer matrix that degrades at a rate effective to sustain release of an amount of the estratopone from the implant effective to treat an ocular condition. The intraocular implant is biodegradable or bioerodible and provides a sustained release of the estratopone in an eye for extended periods of time, such as for more than one week, for example for about three months or more and up to about six months or more. Implants containing an estratopone may or may not include a polyvinyl alcohol.

In another embodiment, the intraocular implants comprise anacortate and a biodegradable polymer matrix, similar to that discussed above.

The biodegradable polymer matrix of the foregoing implants may be a mixture of biodegradable polymers or the matrix may comprise a single type of biodegradable polymer. For example, the matrix may comprise a polymer selected from the group consisting of polylactides, poly (lactide-co-glycolides), and combinations thereof.

A method of making the present implants involves combining or mixing the anti-angiogenic compound with a biodegradable polymer or polymers. The mixture may then be extruded or compressed to form a single composition. The single composition may then be processed to form individual implants suitable for placement in an eye of a patient.

The implants may be placed in an ocular region to treat a variety of ocular conditions, such as treating, preventing, or reducing at least one symptom associated with neovascularization, angiogenesis, tumor growth, and the like.

Kits in accordance with the present invention may comprise one or more of the present implants, and instructions for using the implants. For example, the instructions may explain how to administer the implants to a patient, and types of conditions that may be treated with the implants. Each and every feature described herein, and each and every combination of two or more of such features, is included within the scope of the present invention provided that the features included in such a combination are not mutually inconsistent. In addition, any feature or combination of features may be specifically excluded from any embodiment of the present invention.

Additional aspects and advantages of the present invention are set forth in the following description and claims, particularly when considered in conjunction with the accompanying drawings.

DRAWINGS

Figure 1 is a graph showing the content uniformity versus formulation number, and is reflective of the potency of each of the formulations. Figure 2 is a graph showing the cumulative release profiles for biodegradable 2-methoxyestradiol containing implants (rods) with different biodegradable polymers in 0.5% β-cyclodextrin solutions at 37°C.

Figure 3 is a graph similar to Figure 2 showing the cumulative release profiles for biodegradable 2-methoxyestradiol containing implants (wafers) with different biodegradable polymers in 0.5% β- cyclodextrin solutions at 37°C.

Figure 4 is a graph similar to Figure 3 showing the cumulative release profiles for biodegradable 2-methoxyestradiol containing implants (rods) with different biodegradable polymers in 0.5% β- cyclodextrin solutions at 37°C. The formulations are 13-17 and 1 and 11 , as discussed herein.

DESCRIPTION

As described herein, controlled and sustained administration of a therapeutic agent through the use of one or more intraocular implants may improve treatment of undesirable ocular conditions. The implants comprise a pharmaceutically acceptable polymeric composition and are formulated to release one or more pharmaceutically active agents, such as an anti-angiogenic agent or agents, for example, estradiol derivatives, estratopones, or anacortate, over an extended period of time. The implants are effective to provide a therapeutically effective dosage of the agent or agents directly to a region of the eye to treat, prevent, and/or reduce one or more symptoms of one or more undesirable ocular conditions. Thus, with a single administration, therapeutic agents will be made available at the site where they are needed and will be maintained for an extended period of time, rather than subjecting the patient to repeated injections or, in the case of self-administered drops, ineffective treatment with only limited bursts of exposure to the active agent or agents. An intraocular implant in accordance with the disclosure herein comprises a therapeutic component and a drug release sustaining component associated with the therapeutic component. In accordance with the present invention, the therapeutic component comprises, consists essentially of, or consists of, an antiangiogenic agent, such as an estradiol derivative or an estratopone or anacortate, or a combination thereof. The drug release sustaining component is associated with the therapeutic component to sustain release of an effective amount of the therapeutic component into an eye in which the implant is placed. The amount of the therapeutic component is released into the eye for a period of time greater than about one week after the implant is placed in the eye, and is effective in treating and/or reducing at least one symptom of one or more ocular conditions, such as neovascularization, angiogenesis, tumor growth, and the like.

Definitions

For the purposes of this description, we use the following terms as defined in this section, unless the context of the word indicates a different meaning. As used herein, an "intraocular implant" refers to a device or element that is structured, sized, or otherwise configured to be placed in an eye. Intraocular implants are generally biocompatible with physiological conditions of an eye and do not cause adverse side effects. Intraocular implants may be placed in an eye without disrupting vision of the eye.

As used herein, a "therapeutic component" refers to a portion of an intraocular implant comprising one or more therapeutic agents or substances used to treat a medical condition of the eye. The therapeutic component may be a discrete region of an intraocular implant, or it may be homogenously distributed throughout the implant. The therapeutic agents of the therapeutic component are typically ophthalmically acceptable, and are provided in a form that does not cause adverse reactions when the implant is placed in an eye.

As used herein, an "estradiol derivative" is a compound that binds tubulin, inhibits microtubule formation, and/or exhibits one or more anti- mitotic properties. The phrase "estradiol derivative" as used herein does not include colchicine.

As used herein, an "estratopone" is a compound derived from 2- methoxyestradiol (Miller et al., Synthesis and Structure-Activity Profiles of A-Homoestranes, the Estratopones, J. Med. Chem, 40:3836-3841 , 1997). Estratopones may also be referred to as A-homoestradiol derivatives. In reference to the disclosure herein, are a specific type of estradiol derivative, and more specifically may be understood to be a hybrid between 2-methoxyestradiol and colchicine.

As used herein, a "drug release sustaining component" refers to a portion of the intraocular implant that is effective to provide a sustained release of the therapeutic agents of the implant. A drug release sustaining component may be a biodegradable polymer matrix, or it may be a coating covering a core region of the implant that comprises a therapeutic component.

As used herein, "associated with" means mixed with, dispersed within, coupled to, covering, or surrounding.

As used herein, an "ocular region" or "ocular site" refers generally to any area of the eyeball, including the anterior and posterior segment of the eye, and which generally includes, but is not limited to, any functional (e.g., for vision) or structural tissues found in the eyeball, or tissues or cellular layers that partly or completely line the interior or exterior of the eyeball. Specific examples of areas of the eyeball in an ocular region include the anterior chamber, the posterior chamber, the vitreous cavity, the choroid, the suprachoroidal space, the conjunctiva, the subconjunctival space, the episcleral space, the intracomeal space, the epicorneal space, the sclera, the pars plana, surgically-induced avascular regions, the macula, and the retina.

As used herein, an "ocular condition" is a disease, ailment or condition which affects or involves the eye or one of the parts or regions of the eye. Broadly speaking the eye includes the eyeball and the tissues and fluids which constitute the eyeball, the periocular muscles (such as the oblique and rectus muscles) and the portion of the optic nerve which is within or adjacent to the eyeball.

An anterior ocular condition is a disease, ailment or condition which affects or which involves an anterior (i.e. front of the eye) ocular region or site, such as a periocular muscle, an eye lid or an eye ball tissue or fluid which is located anterior to the posterior wall of the lens capsule or ciliary muscles. Thus, an anterior ocular condition primarily affects or involves the conjunctiva, the cornea, the anterior chamber, the iris, the posterior chamber (behind the retina but in front of the posterior wall of the lens capsule), the lens or the lens capsule and blood vessels and nerve which vascularize or innervate an anterior ocular region or site. Thus, an anterior ocular condition can include a disease, ailment or condition, such as for example, aphakia; pseudophakia; astigmatism; blepharospasm; cataract; conjunctival diseases; conjunctivitis; corneal diseases;, corneal ulcer; dry eye syndromes; eyelid diseases; lacrimal apparatus diseases; lacrimal duct obstruction; myopia; presbyopia; pupil disorders; refractive disorders and strabismus. Glaucoma can also be considered to be an anterior ocular condition because a clinical goal of glaucoma treatment can be to reduce a hypertension of aqueous fluid in the anterior chamber of the eye (i.e. reduce intraocular pressure). A posterior ocular condition is a disease, ailment or condition which primarily affects or involves a posterior ocular region or site such as choroid or sclera (in a position posterior to a plane through the posterior wall of the lens capsule), vitreous, vitreous chamber, retina, optic nerve (i.e. the optic disc), and blood vessels and nerves which vascularize or innervate a posterior ocular region or site. Thus, a posterior ocular condition can include a disease, ailment or condition, such as for example, acute macular neuroretinopathy; Behcet's disease; choroidal neovascularization; diabetic uveitis; histoplasmosis; infections, such as fungal or viral-caused infections; macular degeneration, such as acute macular degeneration, non- exudative age related macular degeneration and exudative age related macular degeneration; edema, such as macular edema, cystoid macular edema and diabetic macular edema; multifocal choroiditis; ocular trauma which affects a posterior ocular site or location; ocular tumors; retinal disorders, such as central retinal vein occlusion, diabetic retinopathy (including proliferative diabetic retinopathy), proliferative vitreoretinopathy (PVR), retinal arterial occlusive disease, retinal detachment, uveitic retinal disease; sympathetic opthalmia; Vogt Koyanagi-Harada (VKH) syndrome; uveal diffusion; a posterior ocular condition caused by or influenced by an ocular laser treatment; posterior ocular conditions caused by or influenced by a photodynamic therapy, photocoagulation, radiation retinopathy, epiretinal membrane disorders, branch retinal vein occlusion, anterior ischemic optic neuropathy, non- retinopathy diabetic retinal dysfunction, retinitis pigmentosa, and glaucoma. Glaucoma can be considered a posterior ocular condition because the therapeutic goal is to prevent the loss of or reduce the occurrence of loss of vision due to damage to or loss of retinal cells or optic nerve cells (i.e. neuroprotection). The term "biodegradable polymer" refers to a polymer or polymers which degrade in vivo, and wherein erosion of the polymer or polymers over time occurs concurrent with or subsequent to release of the therapeutic agent. Specifically, hydrogels such as methylcellulose which act to release drug through polymer swelling are specifically excluded from the term "biodegradable polymer". The terms "biodegradable" and "bioerodible" are equivalent and are used interchangeably herein. A biodegradable polymer may be a homopolymer, a copolymer, or a polymer comprising more than two different polymeric units.

The term "treat", "treating", or "treatment" as used herein, refers to reduction or resolution or prevention of an ocular condition, ocular injury or damage, or to promote healing of injured or damaged ocular tissue.

The term "therapeutically effective amount" as used herein, refers to the level or amount of agent needed to treat an ocular condition, or reduce or prevent ocular injury or damage without causing significant negative or adverse side effects to the eye or a region of the eye.

Intraocular implants have been developed which can release drug loads over various' time periods. These implants, which when inserted into an eye, such as the vitreous of an eye, provide therapeutic levels of an antiangiogenic compound, such as an estradiol derivative or an estratopone or anacortate for extended periods of time (e.g., for about 1 week or more). The disclosed implants are effective in treating ocular conditions, such as posterior ocular conditions, including neovascularization, tumors, angiogenesis and the like.

In one embodiment of the present invention, an intraocular implant comprises a biodegradable polymer matrix. The biodegradable polymer matrix is one type of a drug release sustaining component. The biodegradable polymer matrix is effective in forming a biodegradable intraocular implant. The biodegradable intraocular implant comprises an estradiol derivative associated with the biodegradable polymer matrix. The matrix degrades at a rate effective to sustain release of an amount of the estradiol derivative for a time greater than about one week from the time in which the implant is placed in ocular region or ocular site, such as the vitreous of an eye. The estradiol derivative of the implant is typically an agent that interacts at the colchicine binding site on a tubulin monomer, which may inhibit tubulin polymerization and angiogenesis. Examples of estradiol derivatives useful in the present implants are described in U.S. Patent No. 5,504,074. In short, an estradiol derivative of the present implants may be a compound represented by the following formula:

wherein:

I. R_a-R_o are defined as follows: A) each R_a, Rb, Re, Ra, Re. Rf, Ri, Rj> Rk, RL, Rm, Ro, independently is -R_{1 f} -ORi, -OCOR₁, -SR₁, -F, -NHR₂, -Br, -I; and R_g is - Rι-ORι, -OCOR₁, -SR1, -F, -NHR2, -Br, -I, or -C_≡CH; or B) each R_a, R , R_c, Rf, Rk, RL, RO, independently is -Ri, -ORi, - OCOR1, -SR1, -F, -NHR₂, -Br, or -I; and each R_d, R_e, Rj, R_m, independently is =0, -Ri, -ORi, -OCOR1, -SR₁, -F, -NHR₂, -Br or -I; and R_g is =O, -Ri, -ORi, -OCOR1, -SRi, -F, -NHR₂, -BR, -I, or -C_≡CH

II. Z' is defined as follows: A) Z' is X, where X is O O >COR„ >CC — R >CC — OR,,

or B) Z' is

— C— χ'_ or — X-— C=, I I Rn _n where R_n is -R_{1 f} -ORi, -SRi, -F, -NHR₂, -BR or -I; and X' is X, as defined above; or X' is >C=O; and

III. Z" is defined as follows: A) Z" is Y, where Y is

-0— , — N f— , >CHR_!, >C=0, >C— (CH₂)_nOR₂, Ri 0 Ri 0 I II I II >C-(CH₂)„-CR_2> >C-(CH₂)_n-C-0R₂₎ Ri OH Ri OH I i I >C— (CH₂)_n-CHR2_I >C— (CH₂)„-CH— OR₂, Ri O Ri OH I II I I >C- H(CH₂)_Λ— CR₂, >C-NH(CH₂)„— CHR₂, Ri OH RI O I I I II >C— H(CH₂)_n-CH— OR₂, >C-NH(CH₂)n— C-OR₂,

>C— NH(CH₂)«— OR₂, >C— H(CH₂)„— R₂, Ri O Ri O I II I II >C(CH2)„-NHCR₂, >C-(CH₂)„-NHC-OR2, Ri OH Ri OH I I I I >C-(CH₂)„-NH-CHR₂, >C-(CH₂)„- H-CHOR₂, or

> fC-(CH₂)„-NH— CH₂OR₂, where n is 0-6; or B) Z" is

— Y— CH— or — CH— Y— I I R_p Rp where R_p is -R_{1 (} ORi, -SRi, -F, -NHR₂, -Br or -I and Y is defined as in III (A); and

IV. provided that when each Rb, Re, Rd, Re, Ri, Rj, Rk, RL, Rm, and Ro is H; R_f is -CH₃ ; R_g is -OH; Z' is >COH; and Z" is >CH2; then R_a is not -H; where, in each formula set forth above, each Ri and R₂ independently is -H, or a substituted or unsubstituted alkyl, alkenyl or alkynl group of up to 6 carbons. In certain implants, the estradiol derivative is 2-methoxyestradiol

(AGN 202231) which is represented by the following formula:

These implants may also include salts of the estradiol derivatives.

Pharmaceutically acceptable acid addition salts of the compounds of the invention are those formed from acids which form non-toxic addition salts containing pharmaceutically acceptable anions, such as the hydrochloride, hydrobromide, hydroiodide, sulfate, or bisulfate, phosphate or acid phosphate, acetate, maleate, fumarate, oxalate, lactate, tartrate, citrate, gluconate, saccharate and p-toluene sulphonate salts.

Thus, the implant may comprise a therapeutic component which comprises, consists essentially of, or consists of an estradiol derivative, such as 2-methoxyestradiol, salts thereof, and mixtures thereof. The biodegradable polymer matrix of such implants is preferably substantially free of polyvinyl alcohol, or in other words, includes no polyvinyl alcohol. In another embodiment, a biodegradable intraocular implant comprises an estratopone and a biodegradable polymer matrix. In such an embodiment, the biodegradable polymer matrix may include a polyvinyl alcohol, but preferably, the matrix is substantially free of a polyvinyl alcohol. Examples of estratopones used in such implants are described in U.S. Patent No. 6,271 ,220, and may be represented by the following formula:

wherein A is a fused tropone having a general formula:

wherein X is selected from the group consisting of hydrogen, hydroxy, carboxy, halogen, nitro, Ci to C₁₂ alkenyl, Ci to C1₂ alkyl, C1 to C12 alkoxy, SR, NR₂, OSO₃ ^", OSO₂NR₂, HNS0₃ ^", NHSO₂NR₂, SSO₃ ^", SSO₂NR₂, etc. wherein R is hydrogen or a Ci to C₆ alkyl. Generally, R is selected to be adjacent to the carbonyl moiety of the tropone. Preferably, X is selected from the group consisting of hydrogen, chloro, bromo, methoxy and ethoxy. Most preferably, in the compounds of the implants, A is a fused tropane having the general formula:

wherein X is as described above.

In certain implants, the estrapone is represented by the following formula:

wherein A is a fused tropone having a general formula:

and wherein X is selected from the group consisting of H, CI, Br, methoxy and ethoxy.

In other implants, the estratopone is a compound having the following formula

wherein A is

and wherein X is selected from the group consisting of chloro and bromo.

In other implants, the estratopone is a compound having the following formula

wherein A is

and X is methoxy.

The foregoing implants may also include estratopone salts and combinations of estratopones and estratopone salts, similar to estradiol derivative containing implants.

Additional estradiol derivatives and estratopones may be obtained using conventional methods, such as by routine chemical synthesis methods known to persons of ordinary skill in the art. Therapeutically effective estradiol derivatives and estratopones may be screened and identified using conventional screening technologies, for example, by determining the amount of inhibition of tubulin polymerization in in vitro assays, or by other assays which may be used in identifying the effectiveness of the compounds above. The estradiol derivative and/or estratopone may be in a particulate or powder form and entrapped by the biodegradable polymer matrix. Usually, estradiol derivative and/or estratopone particles in intraocular implants will have an effective average size less than about 3000 nanometers. In certain implants, the particles may have an effective average particle size about an order of magnitude smaller than 3000 nanometers. For example, the particles may have an effective average particle size of less than about 500 nanometers. In additional implants, the particles may have an effective average particle size of less than about 400 nanometers, and in still further embodiments, a size less than about 200 nanometers.

The estradiol derivative and/or estratopone of the implant is preferably from about 10% to 90% by weight of the implant. More preferably, the estradiol derivative and/or estratopone is from about 20% to about 80% by weight of the implant. In a preferred embodiment, the estradiol derivative and/or estratopone comprises about 40% by weight of the implant (e.g., 30%-50%). In another embodiment, the estradiol derivative and/or estratopone comprises about 60% by weight of the implant.

Suitable polymeric materials or compositions for use in the implant include those materials which are compatible, that is biocompatible, with the eye so as to cause no substantial interference with the functioning or physiology of the eye. Such materials preferably are at least partially and more preferably substantially completely biodegradable or bioerodible.

Examples of useful polymeric materials include, without limitation, such materials derived from and/or including organic esters and organic ethers, which when degraded result in physiologically acceptable degradation products, including the monomers. Also, polymeric materials derived from and/or including, anhydrides, amides, orthoesters and the like, by themselves or in combination with other monomers, may also find use. The polymeric materials may be addition or condensation polymers, advantageously condensation polymers. The polymeric materials may be cross-linked or non-cross-linked, for example not more than lightly cross-linked, such as less than about 5%, or less than about 1% of the polymeric material being cross-linked. For the most part, besides carbon and hydrogen, the polymers will include at least one of oxygen and nitrogen, advantageously oxygen. The oxygen may be present as oxy, e.g. hydroxy or ether, carbonyl, e.g. non-oxo-carbonyl, such as carboxylic acid ester, and the like. The nitrogen may be present as amide, cyano and amino. The polymers set forth in Heller, Biodegradable Polymers in Controlled Drug Delivery, In: CRC Critical Reviews in Therapeutic Drug Carrier Systems, Vol. 1 , CRC Press, Boca Raton, FL 1987, pp 39-90, which describes encapsulation for controlled drug delivery, may find use in the present implants.

Of additional interest are polymers of hydroxyaliphatic carboxylic acids, either homopolymers or copolymers, and polysaccharides. Polyesters of interest include polymers of D-lactic acid, L-lactic acid, racemic lactic acid, glycolic acid, polycaprolactone, and combinations thereof. Generally, by employing the L-lactate or D-lactate, a slowly eroding polymer or polymeric material is achieved, while erosion is substantially enhanced with the lactate racemate.

Among the useful polysaccharides are, without limitation, calcium alginate, and functionalized celluloses, particularly carboxymethylcellulose esters characterized by being water insoluble, a molecular weight of about 5 kD to 500 kD, for example.

Other polymers of interest include, without limitation, polyvinyl alcohol, such as for implants that comprise an estratopone, polyesters, polyethers and combinations thereof which are biocompatible and may be biodegradable and/or bioerodible. As discussed herein, when an implant comprises 2-methoxyestradiol or other estradiol derivative, the implant is substantially free of polyvinyl alcohol. Some preferred characteristics of the polymers or polymeric materials for use in the present invention may include biocompatibility, compatibility with the therapeutic component, ease of use of the polymer in making the drug delivery systems of the present invention, a half-life in the physiological environment of at least about 6 hours, preferably greater than about one day, not significantly increasing the viscosity of the vitreous, and water insolubility.

The biodegradable polymeric materials which are included to form the matrix are desirably subject to enzymatic or hydrolytic instability. Water soluble polymers may be cross-linked with hydrolytic or biodegradable unstable cross-links to provide useful water insoluble polymers. The degree of stability can be varied widely, depending upon the choice of monomer, whether a homopolymer or copolymer is employed, employing mixtures of polymers, and whether the polymer includes terminal acid groups.

Equally important to controlling the biodegradation of the polymer and hence the extended release profile of the implant is the relative average molecular weight of the polymeric composition employed in the implant. Different molecular weights of the same or different polymeric compositions may be included in the implant to modulate the release profile. In certain implants, the relative average molecular weight of the polymer will range from about 9 to about 64 kD, usually from about 10 to about 54 kD, and more usually from about 12 to about 45 kD.

In some implants, copolymers of glycolic acid and lactic acid are used, where the rate of biodegradation is controlled by the ratio of glycolic acid to lactic acid. The most rapidly degraded copolymer has roughly equal amounts of glycolic acid and lactic acid. Homopolymers, or copolymers having ratios other than equal, are more resistant to degradation. The ratio of glycolic acid to lactic acid will also affect the brittleness of the implant, where a more flexible implant is desirable for larger geometries. The % of polylactic acid in the polylactic acid polyglycolic acid (PLGA) copolymer can be 0-100%, preferably about 15-85%, more preferably about 35-65%. In some implants, a 50/50 PLGA copolymer is used.

The biodegradable polymer matrix of the intraocular implant may comprise a mixture of two or more biodegradable polymers. For example, the implant may comprise a mixture of a first biodegradable polymer and a different second biodegradable polymer. One or more of the biodegradable polymers may have terminal acid groups.

Release of a drug from an erodible polymer is the consequence of several mechanisms or combinations of mechanisms. Some of these mechanisms include desorption from the implants surface, dissolution, diffusion through porous channels of the hydrated polymer and erosion. Erosion can be bulk or surface or a combination of both. As discussed herein, the matrix of the intraocular implant may release drug at a rate effective to sustain release of an amount of the estradiol derivative and/or estratopone for more than one week after implantation into an eye. In certain implants, therapeutic amounts of the estradiol derivative and/or estratopone are released for more than about one month, and even for about six months or more. One example of the biodegradable intraocular implant comprises

2-methoxyestradiol associated with a biodegradable polymer matrix that is substantially free of polyvinyl alcohol, and comprises a poly (lactide- co-glycolide) or a poly (D,L-lactide-co-glycolide). The implant may have an amount of 2-methoxyestradiol from about 40% to about 70% by weight of the implant. Such a mixture is effective in sustaining release of a therapeutically effective amount of the 2-methoxyestradiol for a time period from about two months to about four months from the time the implant is placed in an eye.

The release of the estradiol derivative and/or estratopone from the intraocular implant comprising a biodegradable polymer matrix may include an initial burst of release followed by a gradual increase in the amount of the estradiol derivative and/or estratopone released, or the release may include an initial delay in release of the estradiol derivative and/or estratopone followed by an increase in release. When the implant is substantially completely degraded, the percent of the estradiol derivative and/or estratopone that has been released is about one hundred. Compared to existing implants, the implants disclosed herein do not completely release, or release about 100% of the estradiol derivative and/or estratopone, until after about one week of being placed in an eye.

It may be desirable to provide a relatively constant rate of release of the estradiol derivative and/or estratopone from the implant over the life of the implant. For example, it may be desirable for the estradiol derivative and/or estratopone to be released in amounts from about 0.01 μg to about 2 μg per day for the life of the implant. However, the release rate may change to either increase or decrease depending on the formulation of the biodegradable polymer matrix. In addition, the release profile of the estradiol derivative and/or estratopone may include one or more linear portions and/or one or more non-linear portions. Preferably, the release rate is greater than zero once the implant has begun to degrade or erode.

The implants may be monolithic, i.e. having the active agent or agents homogenously distributed through the polymeric matrix, or encapsulated, where a reservoir of active agent is encapsulated by the polymeric matrix. Due to ease of manufacture, monolithic implants are usually preferred over encapsulated forms. However, the greater control afforded by the encapsulated, reservoir-type implant may be of benefit in some circumstances, where the therapeutic level of the drug falls within a narrow window. In addition, the therapeutic component, including the estradiol derivative and/or estratopone, may be distributed in a non- homogenous pattern in the matrix. For example, the implant may include a portion that has a greater concentration of the estradiol derivative and/or estratopone relative to a second portion of the implant. The intraocular implants disclosed herein may have a size of between about 5 μm and about 2 mm, or between about 10 μm and about 1 mm for administration with a needle, greater than 1 mm, or greater than 2 mm, such as 3 mm or up to 10 mm, for administration by surgical implantation. The vitreous chamber in humans is able to accommodate relatively large implants of varying geometries, having lengths of, for example, 1 to 10 mm. The implant may be a cylindrical pellet (e. g., rod) with dimensions of about 2 mm x 0.75 mm diameter. Or the implant may be a cylindrical pellet with a length of about 7 mm to about 10 mm, and a diameter of about 0.75 mm to about 1.5 mm.

The implants may also be at least somewhat flexible so as to facilitate both insertion of the implant in the eye, such as in the vitreous, and accommodation of the implant. The total weight of the implant is usually about 250-5000 μg, more preferably about 500-1000 μg. For example, an implant may be about 500 μg, or about 1000 μg. For non- human individuals, the dimensions and total weight of the implant(s) may be larger or smaller, depending on the type of individual. For example, humans have a vitreous volume of approximately 3.8 ml, compared with approximately 30 ml for horses, and approximately 60-100 ml for elephants. An implant sized for use in a human may be scaled up or down accordingly for other animals, for example, about 8 times larger for an implant for a horse, or about, for example, 26 times larger for an implant for an elephant.

Thus, implants can be prepared where the center may be of one material and the surface may have one or more layers of the same or a different composition, where the layers may be cross-linked, or of a different molecular weight, different density or porosity, or the like. For example, where it is desirable to quickly release an initial bolus of drug, the center may be a polylactate coated with a polylactate-polyglycolate copolymer, so as to enhance the rate of initial degradation. Alternatively, the center may be polyvinyl alcohol coated with polylactate, so that upon degradation of the polylactate exterior the center would dissolve and be rapidly washed out of the eye. The implants may be of any geometry including fibers, sheets, films, microspheres, spheres, circular discs, plaques and the like. The upper limit for the implant size will be determined by factors such as toleration for the implant, size limitations on insertion, ease of handling, etc. Where sheets or films are employed, the sheets or films will be in the range of at least about 0.5 mm x 0.5 mm, usually about 3-10 mm x 5-10 mm with a thickness of about 0.1-1.0 mm for ease of handling. Where fibers are employed, the fiber diameter will generally be in the range of about 0.05 to 3 mm and the fiber length will generally be in the range of about 0.5-10 mm. Spheres may be in the range of about 0.5 μm to 4 mm in diameter, with comparable volumes for other shaped particles.

The size and form of the implant can also be used to control the rate of release, period of treatment, and drug concentration at the site of implantation. Larger implants will deliver a proportionately larger dose, but depending on the surface to mass ratio, may have a slower release rate. The particular size and geometry of the implant are chosen to suit the site of implantation.

The proportions of estradiol derivative and/or estratopone, polymer, and any other modifiers may be empirically determined by formulating several implants with varying proportions. A USP approved method for dissolution or release test can be used to measure the rate of release (USP 23; NF 18 (1995) pp. 1790-1798). For example, using the infinite sink method, a weighed sample of the implant is added to a measured volume of a solution containing 0.9% NaCI in water, where the solution volume will be such that the drug concentration is after release is less than 5% of saturation. The mixture is maintained at 37°C and stirred slowly to maintain the implants in suspension. The appearance of the dissolved drug as a function of time may be followed by various methods known in the art, such as spectrophotometrically, HPLC, mass spectroscopy, etc. until the absorbance becomes constant or until greater than 90% of the drug has been released.

In addition to the estradiol derivative and/or estratopone included in the intraocular implants disclosed herein, the intraocular implants may also include one or more additional ophthalmically acceptable therapeutic agents. For example, the implant may include one or more antihistamines, one or more antibiotics, one or more beta blockers, one or more steroids, one or more antineoplastic agents, one or more immunosuppressive agents, one or more antiviral agents, one or more antioxidant agents, and mixtures thereof.

Pharmacologic or therapeutic agents which may find use in the present systems, include, without limitation, those disclosed in U.S. Pat. Nos. 4,474,451 , columns 4-6 and 4,327,725, columns 7-8. Examples of antihistamines include, and are not limited to, loradatine, hydroxyzine, diphenhydramine, chlorpheniramine, brompheniramine, cyproheptadine, terfenadine, clemastine, triprolidine, carbinoxamine, diphenylpyraline, phenindamine, azatadine, tripelennamine, dexchlorpheniramine, dexbrompheniramine, methdilazine, and trimprazine doxylamine, pheniramine, pyrilamine, chiorcyclizine, thonzylamine, and derivatives thereof.

Examples of antibiotics include without limitation, cefazolin, cephradine, cefaclor, cephapirin, ceftizoxime, cefoperazone, cefotetan, cefutoxime, cefotaxime, cefadroxil, ceftazidime, cephalexin, cephalothin,, cefamandole, cefoxitin, cefonicid, ceforanide, ceftriaxone, cefadroxil, cephradine, cefuroxime, ampicillin, amoxicillin, cyclacillin, ampicillin, penicillin G, penicillin V potassium, piperacillin, oxacillin, bacampicillin, cloxacillin, ticarcillin, azlocillin, carbenicillin, methicillin, nafcillin, erythromycin, tetracycline, doxycycline, minocycline, aztreonam, chloramphenicol, ciprofloxacin hydrochloride, clindamycin, metronidazole, gentamicin, lincomycin, tobramycin, vancomycin, polymyxin B sulfate, colistimethate, colistin, azithromycin, augmentin, sulfamethoxazole, trimethoprim, and derivatives thereof.

Examples of beta blockers include acebutolol, atenolol, labetalol, metoprolol, propranolol, timolol, and derivatives thereof. Examples of steroids include corticosteroids, such as cortisone, prednisolone, flurometholone, dexamethasone, medrysone, loteprednol, fluazacort, hydrocortisone, prednisone, betamethasone, prednisone, methylprednisolone, riamcinolpne hexacatonide, paramethasone acetate, diflorasone, fluocinonide, fluocinolone, triamcinolone, derivatives thereof, and mixtures thereof. Examples of antineoplastic agents include adriamycin, cyclophosphamide, actinomycin, bleomycin, duanorubicin, doxorubicin, epirubicin, mitomycin, methotrexate, fluorouracil, carboplatin, carmustine (BCNU), methyl-CCNU, cisplatin, etoposide, interferons, camptothecin and derivatives thereof, phenesterine, taxol and derivatives thereof, taxotere and derivatives thereof, vinblastine, vincristine, tamoxifen, etoposide, piposulfan, cyclophosphamide, and flutamide, and derivatives thereof. Examples of immunosuppresive agents include cyclosporine, azathioprine, tacrolimus, and derivatives thereof.

Examples of antiviral agents include interferon gamma, zidovudine, amantadine hydrochloride, ribavirin, acyclovir, valciclovir, dideoxycytidine, phosphonoformic acid, ganciclovir and derivatives thereof.

Examples of antioxidant agents include ascorbate, alpha- tocopherol, mannitol, reduced glutathione, various carotenoids, cysteine, uric acid, taurine, tyrosine, superoxide dismutase, lutein, zeaxanthin, cryotpxanthin, astazanthin, lycopene, N-acetyl-cysteine, camosine, gamma-glutamylcysteine, quercitin, lactoferrin, dihydrolipoic acid, citrate, Ginkgo Biloba extract, tea catechins, bilberry extract, vitamins E or esters of vitamin E, retinyl palmitate, and derivatives thereof.

Other therapeutic agents include squalamine, carbonic anhydrase inhibitors, alpha agonists, prostamides, prostaglandins, antiparasitics, antifungals, and derivatives thereof. The amount of active agent or agents employed in the implant, individually or in combination, will vary widely depending on the effective dosage required and the desired rate of release from the implant. As indicated herein, the agent will be at least about 1 , more usually at least about 10 weight percent of the implant, and usually not more than about 80, more usually not more than about 40 weight percent of the implant. In addition to the therapeutic component, the intraocular implants disclosed herein may include effective amounts of buffering agents, preservatives and the like. Suitable water soluble buffering agents include, without limitation, alkali and alkaline earth carbonates, phosphates, bicarbonates, citrates, borates, acetates, succinates and the like, such as sodium phosphate, citrate, borate, acetate, bicarbonate, carbonate and the like. These agents advantageously present in amounts sufficient to maintain a pH of the system of between about 2 to about 9 and more preferably about 4 to about 8. As such the buffering agent may be as much as about 5% by weight of the total implant. Suitable water soluble preservatives include sodium bisulfite, sodium bisulfate, sodium thiosulfate, ascorbate, benzalkonium chloride, chlorobutanol, thimerosal, phenylmercuric acetate, phenylmercuric borate, phenylmercuric nitrate, parabens, methylparaben, polyvinyl alcohol, benzyl alcohol, phenylethanol and the like and mixtures thereof. These agents may be present in amounts of from 0.001 to about 5% by weight and preferably 0.01 to about 2% by weight.

In addition, the implants may include a solubility enhancing component provided in an amount effective to enhance the solubility of the estradiol derivative and/or estratopone relative to substantially identical implants without the solubility enhancing component. For example, an implant may include a β-cyclodextrin, which is effective in enhancing the solubility of the estradiol derivative and/or estratopone. The β-cyclodextrin may be provided in an amount from about 0.5% (w/w) to about 25% (w/w) of the implant. In certain implants, the β-cyclodextrin is provided in an amount from about 5% (w/w) to about 15% (w/w) of the implant In some situations mixtures of implants may be utilized employing the same or different pharmacological agents. In this way, a cocktail of release profiles, giving a biphasic or triphasic release with a single administration is achieved, where the pattern of release may be greatly varied.

Additionally, release modulators such as those described in U. S. Patent No. 5,869,079 may be included in the implants. The amount of release modulator employed will be dependent on the desired release profile, the activity of the modulator, and on the release profile of the estradiol derivative or estratopone in the absence of modulator. Electrolytes such as sodium chloride and potassium chloride may also be included in the implant. Where the buffering agent or enhancer is hydrophilic, it may also act as a release accelerator. Hydrophilic additives act to increase the release rates through faster dissolution of the material surrounding the drug particles, which increases the surface area of the drug exposed, thereby increasing the rate of drug bioerosion. Similarly, a hydrophobic buffering agent or enhancer dissolve more slowly, slowing the exposure of drug particles, and thereby slowing the rate of drug bioerosion.

Various techniques may be employed to produce the implants described herein. Useful techniques include, but are not necessarily limited to, solvent evaporation methods, phase separation methods, interfacial methods, molding methods, injection molding methods, extrusion methods, co-extrusion methods, carver press method, die cutting methods, heat compression, combinations thereof and the like. Specific methods are discussed in U.S. Pat. No. 4,997,652.

Extrusion methods may be used to avoid the need for solvents in manufacturing. When using extrusion methods, the polymer and drug are chosen so as to be stable at the temperatures required for manufacturing, usually at least about 85 degrees Celsius. Extrusion methods use temperatures of about 25 degrees C to about 150 degrees C, more preferably about 65 degrees C to about 130 degrees C. An implant may be produced by bringing the temperature to about 60 degrees C to about 150 degrees C for drug/polymer mixing, such as about 130 degrees C, for a time period of about 0 to 1 hour, 0 to 30 minutes, or 5-15 minutes. For example, a time period may be about 10 minutes, preferably about 0 to 5 min. The implants are then extruded at a temperature of about 60 degrees C to about 130 degrees C, such as about 75 degrees C.

In addition, the implant may be coextruded so that a coating is formed over a core region during the manufacture of the implant.

Compression methods may be used to make the implants, and typically yield implants with faster release rates than extrusion methods. Compression methods may use pressures of about 50-150 psi, more preferably about 70-80 psi, even more preferably about 76 psi, and use temperatures of about 0 degrees C to about 115 degrees C, more preferably about 25 degrees C.

The implants of the present invention may be inserted into the eye, for example the vitreous chamber of the eye, by a variety of methods, including placement by forceps or by trocar following making a 2-3 mm incision in the sclera. One example of a device that may be used to insert the implants into an eye is disclosed in U.S. Patent Publication No. 2004/0054374. The method of placement may influence the therapeutic component or drug release kinetics. For example, delivering the implant with a trocar may result in placement of the implant deeper within the vitreous than placement by forceps, which may result in the implant being closer to the edge of the vitreous. The location of the implant may influence the concentration gradients of therapeutic component or drug surrounding the element, and thus influence the release rates (e.g., an element placed closer to the edge of the vitreous may result in a slower release rate).

The present implants are configured to release an amount of the estradiol derivative or estratopone effective to treat or reduce an ocular condition, such as an ocular condition related to angiogenesis, neovascularization, and mitosis. More specifically, the implants may be used in a method to reduce neovascularization and treat ocular tumors.

The implants disclosed herein may also be configured to release the estradiol derivative or estratopone or additional therapeutic agents, as described above, which to prevent diseases or conditions, such as the following:

MACULOPATHIES/RETINAL DEGENERATION: Non-Exudative Age Related Macular Degeneration (ARMD), Exudative Age Related Macular Degeneration (ARMD), Choroidal Neovascularization, Diabetic Retinopathy, Acute Macular Neuroretinopathy, Central Serous

Chorioretinopathy, Cystoid Macular Edema, Diabetic Macular Edema.

UVEITIS/RETINITIS/CHOROIDITIS: Acute Multifocal Placoid Pigment Epitheliopathy, Behcet's Disease, Birdshot Retinochoroidopathy, Infectious (Syphilis, Lyme, Tuberculosis, Toxoplasmosis), Intermediate Uveitis (Pars Planitis), Multifocal Choroiditis, Multiple Evanescent White Dot Syndrome (MEWDS), Ocular Sarcoidosis, Posterior Scleritis, Serpignous Choroiditis, Subretinal Fibrosis and Uveitis Syndrome, Vogt-Koyanagi-Harada Syndrome.

VASCULAR DISEASES/EXUDATIVE DISEASES: Coat's Disease, Parafoveal Telangiectasis, Papillophlebitis, Frosted Branch Angitis, Sickle Cell Retinopathy and other Hemoglobinopathies, Angioid Streaks, Familial Exudative Vitreoretinopathy.

TRAUMATIC/SURGICAL: Sympathetic Ophthalmia, Uveitic Retinal Disease, Retinal Detachment, Trauma, Laser, PDT, Photocoagulation, Hypoperfusion During Surgery, Radiation Retinopathy, Bone Marrow Transplant Retinopathy.

PROLIFERATIVE DISORDERS: Proliferative Vitreal Retinopathy and Epiretinal Membranes, Proliferative Diabetic Retinopathy, Retinopathy of Prematurity (retrolental fibroplastic).

INFECTIOUS DISORDERS: Ocular Histoplasmosis, Ocular Toxocariasis, Presumed Ocular Histoplasmosis Syndrome (POHS), Endophthalmitis, Toxoplasmosis, Retinal Diseases Associated with HIV Infection, Choroidal Disease Associated with HIV Infection, Uveitic Disease Associated with HIV Infection, Viral Retinitis, Acute Retinal Necrosis, Progressive Outer Retinal Necrosis, Fungal Retinal Diseases, Ocular Syphilis, Ocular Tuberculosis, Diffuse Unilateral Subacute Neuroretinitis, Myiasis.

GENETIC DISORDERS: Systemic Disorders with Accosiated Retinal Dystrophies, Congenital Stationary Night Blindness, Cone Dystrophies, Fundus Flavimaculatus, Best's Disease, Pattern Dystrophy of the Retinal Pigmented Epithelium, X-Linked Retinoschisis, Sorsby's Fundus Dystrophy, Benign Concentric Maculopathy, Bietti's Crystalline Dystrophy, pseudoxanthoma elasticum, Osier Weber syndrome.

RETINAL TEARS/HOLES: Retinal Detachment, Macular Hole, Giant Retinal Tear. TUMORS: Retinal Disease Associated with Tumors, Solid Tumors, Tumor Metastasis, Benign Tumors, for example, hemangiomas, neurofibromas, trachomas, and pyogenic granulomas, Congenital Hypertrophy of the RPE, Posterior Uveal Melanoma, Choroidal Hemangioma, Choroidal Osteoma, Choroidal Metastasis, Combined Hamartoma of the Retina and Retinal Pigmented Epithelium, Retinoblastoma, Vasoproliferative Tumors of the Ocular Fundus, Retinal Astrocytoma, Intraocular Lymphoid Tumors. MISCELLANEOUS: Punctate Inner Choroidopathy, Acute

Posterior Multifocal Placoid Pigment Epitheliopathy, Myopic Retinal Degeneration, Acute Retinal Pigment Epithelitis, Ocular inflammatory and immune disorders, ocular vascular malfunctions, Corneal Graft Rejection, Neovascular Glaucoma and the like.

In one embodiment, an implant, such as the implants disclosed herein, is administered to a posterior segment of an eye of a human or animal patient, and preferably, a living human or animal. In at least one embodiment, an implant is administered without accessing the subretinal space of the eye. For example, a method of treating a patient may include placing the implant directly into the posterior chamber of the eye. In other embodiments, a method of treating a patient may comprise administering an implant to the patient by at least one of intravitreal injection, subconjuctival injection, sub-tenon injections, retrobulbar injection, and suprachoroidal injection.

In at least one embodiment, a method of reducing neovascularization or angiogenesis in a patient comprises administering one or more implants containing one or more estradiol derivatives or estratopones, as disclosed herein to a patient by at least one of intravitreal injection, subconjuctival injection, sub-tenon injection, retrobulbar injection, and suprachoroidal injection. A syringe apparatus including an appropriately sized needle, for example, a 22 gauge needle, a 27 gauge needle or a 30 gauge needle, can be effectively used to inject the composition with the posterior segment of an eye of a human or animal. Repeat injections are often not necessary due to the extended release of the estradiol derivative or estratopone from the implants.

In another aspect of the invention, kits for treating an ocular condition of the eye are provided, comprising: a) a container comprising an extended release implant comprising a therapeutic component including an estradiol derivative, such as 2-methoxyestradiol, or an estratopone, and a drug release sustaining component; and b) instructions for use. Instructions may include steps of how to handle the implants, how to insert the implants into an ocular region, and what to expect from using the implants.

Example 1

Manufacture and testing of implants containing an estradiol derivative or estratopone and a biodegradable polymer matrix

Biodegradable implants are made by combining 2- methoxyestradiol or an estrapone represented by any of the estrapone formulas above with a biodegradable polymer composition in a stainless steel mortar. The combination is mixed via a Turbula shaker set at 96 RPM for 15 minutes. The powder blend is scraped off the wall of the mortar and then remixed for an additional 15 minutes. The mixed powder blend is heated to a semi-molten state at specified temperature for a total of 30 minutes, forming a polymer/drug melt.

Rods are manufactured by pelletizing the polymer/drug melt using a 9 gauge polytetrafluoroethylene (PTFE) tubing, loading the pellet into the barrel and extruding the material at the specified core extrusion temperature into filaments. The filaments are then cut into about 1 mg size implants or drug delivery systems. The rods have dimensions of about 2 mm long x 0.72 mm diameter. The rod implants weigh between about 900 μg and 1100 μg.

Wafers are formed by flattening the polymer melt with a Carver press at a specified temperature and cutting the flattened material into wafers, each weighing about 1 mg. The wafers have a diameter of about 2.5 mm and a thickness of about 0.13 mm. The wafer implants weigh between about 900 μg and 1100 μg.

In-vitro release testing can be performed on each lot of implant (rod or wafer). Each implant may be placed into a 24 mL screw cap vial with 10 mL of Phosphate Buffered Saline solution at 37°C and 1 mL aliquots are removed and replaced with equal volume of fresh medium on day 1 , 4, 7, 14, 28, and every two weeks thereafter.

Drug assays may be performed by HPLC, which consists of a Waters 2690 Separation Module (or 2696), and a Waters 2996

Photodiode Array Detector. An Ultrasphere, C-18 (2), 5 μm; 4.6 x 150 mm column heated at 30 ° C can be used for separation and the detector can be set at 264 nm. The mobile phase can be (10:90) MeOH - buffered mobile phase with a flow rate of 1 mUmin and a total run time of 12 min per sample. The buffered mobile phase may comprise

(68:0.75:0.25:31) 13 mM 1 -Heptane Sulfonic Acid, sodium salt - glacial acetic acid - triethylamine - Methanol. The release rates can be determined by calculating the amount of drug being released in a given volume of medium over time in μg/day.

The polymers chosen for the implants can be obtained from Boehringer Ingelheim or Purac America, for example. Examples of polymers include: RG502, RG752, R202H, R203 and R206, and Purac PDLG (50/50). RG502 is (50:50) poly(D,L-lactide-co-glycolide), RG752 is (75:25) poly(D,L-lactide-co-glycolide), R202H is 100% poly(D, L- lactide) with acid end group or terminal acid groups, R203 and R206 are both 100% poly(D, L-lactide). Purac PDLG (50/50) is (50:50) poly(D,L- lactide-co-glycolide). The inherent viscosity of RG502, RG752, R202H, R203, R206 , and Purac PDLG are 0.2, 0.2, 0.2, 0.3, 1.0, and 0.2 dL/g, respectively. The average molecular weight of RG502, RG752, R202H, R203, R206, and Purac PDLG are, 11700, 11200, 6500, 14000, 63300, and 9700 daltons, respectively.

Example 2 Methods of making particle biodegradable implants containing 2ME2 2ME2 (AGN 202231) was obtained from Allergan, and its particle size was reduced to approximately 40 μm by a ball mill (MM200, Retsch, USA) before use. PLGA/PLA raw materials were obtained from Boehringer Ingelheim, Inc, Purac America Inc. or Birmingham Polymers, Inc.

Example 3 In vitro release of 2ME2 from biodegradable implants

2ME2 release was examined in a 0.05 M KH PO₄ solution (pH 4.4) with 0.5% β-Cyclodextrin (β-CD) in a shaking water bath (Precision) at 37°C. The 2ME2 drug delivery systems were incubated in 20 mL of medium. The medium was totally replaced with fresh medium at each sampling time. Drug concentrations were determined by HPLC consisting of a Waters 2690 Separation Module equipped with a Symmetry C18 column (4.6x75 mm, 3.5μm, equilibrated at ambient temperature) and a Waters 996 photodiode array detector (set at 285 nm) using 1 % acetic acid in acetonitrile/water (35/65 by volume) as the mobile phase under a flow rate of 1.0 mLAnin (2). The column was equilibrated with mobile phase for at least 30 min before initiating any sample injection.

The solubility of 2ME2 in various solvents was determined by incubating excess 2ME2 in the individual solvent, sonicating, and subjecting the sample to filtration and HPLC assay as stated above. To determine potency, the DDS was dissolved in acetonitrile and diluted to an appropriate concentration by mobile phase before injection into the HPLC.

The solubility of 2ME2 was determined in various solvents and the results are summarized in Table 1. It appears that 2ME2 in 0.5% β- Cyclodextrin (β-CD) in potassium phosphate (KH₂PO₄), 0.5% β-CD in potassium acetate (KOAc), and 0.5% sodium dodecyl sulfate (SDS) in saline demonstrated good solubility. However, the KH₂PO₄ solution exhibited good pH stability of 2ME2, and so 0.5% β-CD in KH₂P0₄ was selected as the release testing medium in this experiment. Table 1. Solubility of 2ME2 in various solvents under ambient temperature (25°C).

The characteristics of the formulations, including formulation identification, drug loading, and product form are summarized in Table 2. The formulations were either extruded from a 750 μm nozzle into filament or hot-pressed into wafer.

The polymers chosen for the implants were obtained from Boehringer Ingelheim, Purac America Inc., or Birmingham Polymers, Inc. The polymers were: RG752, RG755, R203, Purac PDLG (50/50), and BPI PLGA. RG752 is (75:25) poly(D,L-lactide-co-glycolide), RG755 is a poly (D,L-lactide-co-glycolide) at a ratio of 75:25 (D,L-lactide:glycolide); R203 is 100% poly(D, L-lactide). Purac PDLG (50/50) is (50:50) poly(D,L-lactide-co-glycolide). The inherent viscosity of RG752, RG755, R203, and Purac PDLG are 0.2, 0.6, 0.3, and 0.2 dlJg, respectively. The average molecular weight of RG752, RG755, R203, and Purac PDLG are, 11200, 40000, 14000, and 9700 daltons, respectively. Table 2. Characteristics of 2ME2 formulations.

The potency of Formulations 1 to 12 (F1 to F12) was determined, and the results are summarized in Figure 1. All formulations demonstrated very good potencies except F2 and F10 that displayed a high (116%) and low (90%) potency, respectively with a relatively large standard deviation. This could result from difficulty in processing the 2ME2 and polymer material(s) during formulation. 2ME2 release from Formulations 1 , 3, 5, 7, 9, and 11 (rod form) in phosphate solution with 0.5% β-CD are presented in Figure 2. F1 and F9 formulations demonstrated similar release profiles. Approximately 20% of 2ME2 was released during the first 60 days and a complete release was achieved during the following 40 days. The low content (5%) of β-CD in F9 did not play a significant role in drug release. Less than 10% of 2ME2 was released from F3 and F7 within 56 days, and therefore the release testing was terminated. The slow release is attributed to the highly hydrophobic polymers. For F5 and F11 , a much faster drug release was observed. Approximately 90% of 2ME2 was released from F5 and F11 in approximately 40 and 60 days, respectively. 2ME2 release from Formulations 2, 4, 6, 8, 10, and 12 (wafer form) in a phosphate buffered solution with 0.5% β-CD is presented in Figure 3. F2 and F10 wafer formulations demonstrated similar release profiles as F1 and F9 (their rod counterpart), as described above. Less than 10% of 2ME2 was released from F4 and F8 within 60 days, whereas a significant burst effect was observed for F4 from day 60 to day 105 and a very limited amount of 2ME2 was continuously released for F8 after day 60. Despite a different geometry, F5 and F6, made from the same polymer, demonstrated similar release profiles. Approximately 90% of 2ME2 was released from F12 in 60 days, similar to F11 (its rod counterpart), with a slow drug release during the first three weeks.

In order to achieve more linear release profiles, further formulation work was conducted. Formulations 13 to 17 were made into a rod form, and their release profiles in a phosphate solution with 0.5% β-CD are shown in Figure 4. For comparison, the release profiles of F1 and F11 were included. Approximately 35% of 2ME2 was released at the first 70 days followed by a complete drug release on day 119 for F13 and F16. It appears that the combined 5% difference in polymer and β- CD ratio between F13 and F16 did not make a significant difference in release profile until 70 days later. F14 demonstrated a more linear release profile and more than 60% was released by day 80. A very slow release phase was found for F15 during the first 4 weeks, and more than 70% of drug was released over the following 3 weeks, and thereafter its release testing was terminated. For F17, more than 60% of the 2ME2 was released during the first 9 weeks, and the drug release was completed after 3 months.

A total of 17 2ME2 (AGN 202231) formulations were made into either rod or wafer form using various PLGA or PLA at different 2ME2 drug loadings. Release medium screening revealed that 0.5% β-CD in KH₂P0₄ solution achieved both good solubility and pH stability of 2ME2. Formulations of rod form demonstrated better potencies than those of wafer form. Similar drug release profiles were found from formulations containing the same ingredients, regardless of their geometry. Relatively consistent drug release could be maintained for 2 months (such as F11) or 4 months (such as F14).

Example 4 Use Of A 2ME2 Containing Intraocular Implant To Treat Proliferative Diabetic Retinopathy During an eye examination, a 48 year diabetic male suffering from diabetic retinopathy receives a diagnosis that neovascularization is occuring near the optic nerve of each of his eyes. The physician recommends treatment with a biodegradable intraocular implant containing 2-methoxyestradiol (2ME2). One (1000 μg) implant containing 500 μg of 2ME2 in 0.5% β-CD is placed in each eye of the patient. The implants are in the form of rods made from a PLGA polymer matrix. Eye examination of the patient is conducted on a weekly basis for 12 months. The neovascularization appears to have been arrested within about 10 days after the implantation of the implants. The patient does not experience any growth of blood vessels over the optic nerve for the entire year.

All references, articles, publications and patents and patent applications cited herein are incorporated by reference in their entireties.

While this invention has been described with respect to various specific examples and embodiments, it is to be understood that the invention is not limited thereto and that it can be variously practiced within the scope of the following claims.

Claims

Docket 17705 (OCU)We claim:

1. A biodegradable intraocular implant comprising: an estradiol derivative and a biodegradable polymer matrix that is substantially free of a polyvinyl alcohol and that releases drug at a rate effective to sustain release of an amount of the estradiol derivative from the implant for at least about one week after the implant is placed in an eye.

2. The implant of claim 1 , wherein the estradiol derivative is a compound having the following formula:

wherein:

I. R_a-Ro are defined as follows: A) each R_a, Rb, Re, Rd, RΘ, Rf, Ri, Rj, Rk, RL, Rm, Ro, independently is -R_{1 f} -ORi, -OCORi, -SRi, -F, -NHR₂, -Br, -I; and R_g is - R_I-ORL -OCORi, -SRi, -F, -NHR₂, -Br, -I, or -C=CH; or B) each R_a, Rb, R_c, Rf, Rk, RL, R_O, independently is -R_{1 f} -ORi, - OCORi, -SRi, -F, -NHR₂, -Br, or -I; and each R_d, R_e, Ri, Rm, independently is =0, -R -ORi, -OCORi, -SRi, -F, -NHR₂, -Br or -I; and R_g is =O, -Ri, -ORi, -OCORi, -SRi, -F, -NHR₂, -BR, -I, or -C=CH

Z' is defined as follows: Docket 17705 (OCU)

A) Z' is X, where X is

O o >COR„ >CC — R,, >CC — OR,,

OH OH I I >CCH R, or >CCH OR,;- or B) Z' is

=C— X¹— or — X^*— C=, I I R n where R_n is -R_{1 f} -ORi, -SRi, -F, -NHR₂, -BR or -I; and X' is X, as defined above; or X' is >C=O; and

III. Z" is defined as follows: A) Z" is Y, where Y is

Docket 17705 (OCU)

Ri — O— , — N— , >CHRι, >C=0, >C f— (CH₂)„OR₂j Rl O R_! O I II I II >C— (CHz),,— CR₂, >C-(CH₂)„-C— OR₂, Ri OH Ri OH I I I I >C— (CH₂)„— CHR₂₎ >C— (CH₂)_n— CH— OR , Rl O Ri OH I II I I >C-mi(CH.ύn— CR₂, >C— NH(CH₂)„— CHR₂, R, OH Ri O I I I 11 >C— NH(CH₂)_Λ— CH— OR₂, >C— NH(CH₂ ,,— C— OR₂,|

>C f— NH(CH2)_Λ-OR2, >C-NH(CH₂)_Λ— R₂, Ri O Ri O I II I II >C(CH2)„— NHCR₂, >C— (CH₂)„— NHC— OR₂,

or

>C-(CH₂)n-NH-CH₂OR₂, where n is 0-6; or B) Z" is

-Y— CH- or -CH— Y— R_D where R_p is -R_{1 f} ORi, -SRi, -F, -NHR₂, -Br or -I and Y is defined as in III (A); and

IV. provided that when each Rb, Re, Rd, Re, Ri, Rj, Rk, RL, Rm, and Ro is H; Docket 17705 (OCU)

R, is -CH₃ ; R_g is -OH; Z' is >COH; and Z" is >CH2; then R_a is not -H; where, in each formula set forth above, each Ri and R₂ independently is -H, or a substituted or unsubstituted alkyl, alkenyl or alkynl group of up to 6 carbons.

3. The implant of claim 1 , wherein the estradiol derivative is 2- methoxyestradiol, salts thereof, and mixtures thereof.

4. The implant of claim 1 , further comprising an additional ophthalmically acceptable therapeutic agent.

5. The implant of claim 1 , wherein the estradiol derivative is dispersed within the biodegradable polymer matrix.

6. The implant of claim 1 , further comprising a solubility enhancing component provided in an amount effective to enhance the solubility of the estradiol derivative relative to an substantially identical implant without the solubility enhancing component.

7. The implant of claim 6, wherein the solubility enhancing component comprises β-cyclodextrin.

8. The implant of claim 7, wherein the β-cyclodextrin is provided in an amount from about 0.5% (w/w) to about 25% (w/w) of the implant.

9. The implant of claim 8, wherein the β-cyclodextrin is provided in an amount from about 5% (w/w) to about 15% (w/w) of the implant. Docket 17705 (OCU)

10. The implant of claim 1 , wherein the matrix comprises at least one polymer selected from the group consisting of polylactides, poly (lactide- co-glycolides), derivatives thereof, and mixtures thereof.

11. The implant of claim 1 , wherein the matrix comprises a poly (lactide-co-glycolide).

12. The implant of claim 1 , wherein the matrix comprises a poly(D,L- lactide-co-glycolide).

13. The implant of claim 1 , wherein the matrix releases drug at a rate effective to sustain release of an amount of the estradiol derivative from the implant for more than one month from the time the implant is placed in the vitreous of the eye.

14. The implant of claim 1 , wherein the estradiol derivative is 2- methoxyestradiol, and the matrix releases drug at a rate effective to sustain release of a therapeutically effective amount of the 2- methoxyestradiol for a time from about two months to about six months.

15. The implant of claim 1 , wherein the implant is structured to be placed in the vitreous of the eye.

16. The implant of claim 1 , wherein the estradiol derivative is a 2- methoxyestradiol provided in an amount from about 40% by weight to about 70% by weight of the implant, and the biodegradable polymer matrix comprises a poly (lactide-co-glycolide) in an amount from about 30% by weight to about 60% by weight of the implant.

17. The implant of claim 1 formed as a rod, a wafer, or a particle.

18. The implant of claim 1 which is formed by an extrusion process. Docket 17705 (OCU)

19. A biodegradable intraocular implant comprising: an estratopone and a biodegradable polymer matrix that releases drug at a rate effective to sustain release of an amount of the estratopone from the implant for at least about one week after the implant is placed in an eye.

20. The implant of claim 19, wherein the estratopone is a compound having the following formula

wherein A is a fused tropone having a general formula:

wherein X is selected from the group consisting of H, CI, Br, methoxy and ethoxy.

21. The implant of claim 19, wherein the estratopone is a compound having the following formula Docket 17705 (OCU)

wherein A is

wherein X is selected from the group consisting of chloro and bromo.

22. The implant of claim 19, wherein the estratopone is a compound having the following formula

wherein A is

and X is methoxy. Docket 17705 (OCU)

23. The implant of claim 19, further comprising an additional ophthalmically acceptable therapeutic agent.

24. The implant of claim 19, wherein the estratopone is dispersed within the biodegradable polymer matrix.

25. The implant of claim 20, further comprising a solubility enhancing component provided in an amount effective to enhance the solubility of the estradiol derivative relative to an substantially identical implant without the solubility enhancing component.

26. The implant of claim 25, wherein the solubility enhancing component comprises β-cyclodextrin.

27. The implant of claim 26, wherein the β-cyclodextrin is provided in an amount from about 0.5% (w/w) to about 25% (w/w) of the implant.

28. The implant of claim 19, wherein the matrix comprises at least one polymer selected from the group consisting of polylactides, poly (lactide-co-glycolides), derivatives thereof, and mixtures thereof.

29. The implant of claim 19, wherein the matrix is substantially free of a polyvinyl alcohol.

30. The implant of claim 19, wherein the matrix releases drug at a rate effective to sustain release of an amount of the estratopone from the implant for more than one month from the time the implant is placed in the vitreous of the eye.

31. The implant of claim 19, wherein the implant is structured to be placed in the vitreous of the eye. Docket 17705 (OCU)

32. The implant of claim 19, wherein the estratopone is provided in an amount from about 20% by weight to about 80% by weight of the implant, and the biodegradable polymer matrix comprises a poly (lactide- co-glycolide) in an amount from about 20% by weight to about 80% by weight of the implant.

33. The implant of claim 19 formed as a rod, a wafer, or a particle.

34. The implant of claim 19 which is formed by an extrusion process.

35. A method of making a biodegradable intraocular implant, comprising the step of: extruding a mixture of an estradiol derivative or an estratopone and a biodegradable polymer component to form a biodegradable material that degrades at a rate effective to sustain release of an amount of the estradiol derivative or an estratopone from the implant for at least about one week after the implant is placed in an eye.

36. The method of claim 35, wherein mixture consists essentially of 2- methoxyestradiol and a biodegradable polymer.

37. The method of claim 35, further comprising a step of mixing the estradiol derivative or estratopone with the polymer component before the extrusion step.

38. The method of claim 35, wherein the estradiol derivative or estratopone and the polymer component are in a powder form.

39. The method of claim 35, wherein the polymer component comprises a polymer selected from the group consisting of polylactides, poly (lactide-co-glycolides), and combinations thereof. Docket 17705 (OCU)

40. The method of claim 35, wherein the polymer component is substantially free of polyvinyl alcohol.

41. A medicament, which is a biodegradable intraocular implant, for treating an ocular condition characterized by undesirable angiogenesis in an eye of a patient, by placing the biodegradable intraocular implant in an eye of the patient, the implant comprising (i) an estradiol derivative and a biodegradable polymer matrix substantially free of polyvinyl alcohol, or (ii) an estratopone and a biodegradable polymer matrix, wherein the implant degrades at a rate effective to sustain release of an amount of the estradiol derivative or estratopone from the implant effective to reduce angiogenesis in the eye of the patient.

42. The medicament of claim 41 , wherein the medicament is effective to treat a retinal ocular condition.

43. The medicament of claim 41 , wherein the ocular condition is a condition selected from the group consisting of ocular tumors, vascular malfunctions, Bechet's disease, diabetic retinopathy, retinopathy of prematurity, macular degeneration, corneal graft rejection, neovascular glaucoma and Osier Weber syndrome.

44. The medicament of claim 41 , wherein the implant is placed in the posterior of the eye.

45. The medicament of claim 41 , wherein the implant is placed in the eye with a trocar.

46. The medicament of claim 41 , wherein the implant is placed in the eye with a syringe. Docket 17705 (OCU)

47. The medicament f claim 41 , wherein a therapeutic agent is administered in addition to the estradiol derivative or estratopone to the patient.

48. The medicament of claim 41 , wherein the estradiol derivative is 2- methoxyestradiol, salts thereof, and mixtures thereof.

49. A biodegradable intraocular implant comprising: an anti-angiogenic agent and a biodegradable polymer matrix that is substantially free of a polyvinyl alcohol and that releases drug at a rate effective to sustain release of an amount of the anti-angiogenic agent from the implant for at least about one week after the implant is placed in an eye.

50. A biodegradable intraocular implant comprising: anacortate and a biodegradable polymer matrix that releases drug at a rate effective to sustain release of an amount of the anacortate from the implant for at least about one week after the implant is placed in an eye.