WO2005108563A3 - Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4 - Google Patents

Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4 Download PDF

Info

Publication number
WO2005108563A3
WO2005108563A3 PCT/US2005/013509 US2005013509W WO2005108563A3 WO 2005108563 A3 WO2005108563 A3 WO 2005108563A3 US 2005013509 W US2005013509 W US 2005013509W WO 2005108563 A3 WO2005108563 A3 WO 2005108563A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptidoglycan
bacteriophage
protein encoded
hydrolyzing protein
hydrolyzing
Prior art date
Application number
PCT/US2005/013509
Other languages
French (fr)
Other versions
WO2005108563A2 (en
Inventor
Emina Stojkovic
Lucia B Rothman-Denes
Original Assignee
Univ Chicago
Emina Stojkovic
Lucia B Rothman-Denes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Chicago, Emina Stojkovic, Lucia B Rothman-Denes filed Critical Univ Chicago
Publication of WO2005108563A2 publication Critical patent/WO2005108563A2/en
Publication of WO2005108563A3 publication Critical patent/WO2005108563A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01092—Peptidoglycan beta-N-acetylmuramidase (3.2.1.92)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention overcomes the limitations of the prior art by providing novel peptidoglycan-hydrolases. In one aspect of the invention the peptidoglycan-hydrolyzing enzyme is gp61 (an N-acetylmuramidase) encoded by ORF61 of bacteriophage N4. The invention provides methods and compositions comprising use of gp6l, as well as homologs thereof. The invention will find use, for example, in the treatment of bacterial infections.
PCT/US2005/013509 2004-04-19 2005-04-19 Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4 WO2005108563A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56332704P 2004-04-19 2004-04-19
US60/563,327 2004-04-19
US58186004P 2004-06-22 2004-06-22
US60/581,860 2004-06-22

Publications (2)

Publication Number Publication Date
WO2005108563A2 WO2005108563A2 (en) 2005-11-17
WO2005108563A3 true WO2005108563A3 (en) 2006-12-07

Family

ID=35320799

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/013509 WO2005108563A2 (en) 2004-04-19 2005-04-19 Peptidoglycan-hydrolyzing protein encoded by bacteriophage n4

Country Status (1)

Country Link
WO (1) WO2005108563A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
WO2010149795A1 (en) 2009-06-26 2010-12-29 Lysando Holding Establishment Antimicrobial agents
EA037202B1 (en) * 2009-06-26 2021-02-18 Катхолике Университейт Лёвен, К.У. Лёвен Р Энд Д Fusion proteins for treating gram-negative bacterial infections

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000029588A1 (en) * 1998-11-18 2000-05-25 Children's Hospital Medical Center Lysozyme fusion proteins in infections
WO2001075067A2 (en) * 2000-03-31 2001-10-11 Hyseq, Inc. Novel nucleic acids and polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000029588A1 (en) * 1998-11-18 2000-05-25 Children's Hospital Medical Center Lysozyme fusion proteins in infections
WO2001075067A2 (en) * 2000-03-31 2001-10-11 Hyseq, Inc. Novel nucleic acids and polypeptides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRANEN JILL K ET AL: "Enhancement of nisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin.", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. 1 JAN 2004, vol. 90, no. 1, 1 January 2004 (2004-01-01), pages 63 - 74, XP002378310, ISSN: 0168-1605 *

Also Published As

Publication number Publication date
WO2005108563A2 (en) 2005-11-17

Similar Documents

Publication Publication Date Title
WO2006013441A3 (en) Use of probiotic bacteria for the preparation of topical compositions for skin protection
TNSN05254A1 (en) Derivatives of 2-acylamino-4-phenylthiazole, preparation method thereof and application of same in therapeutics
WO2005072826A3 (en) Compositions and use of tyrosine kinase inhibitors to treat pathogenic infection
WO2004069156A3 (en) Inactivated probiotic bacteria and methods of use thereof
WO2005012242A3 (en) Substituted benzimidazole-, benztriazole-, and benzimidazolone-o-glucosides
WO2003099217A3 (en) Methods of treating bacterial infections and diseases associated therewith
WO2004087145A3 (en) Antibacterial agents
WO2005055944A3 (en) Oligosaccharide compositions and use thereof in the treatment of infection
WO2008153610A3 (en) Use of il-23 antagonists for treatment of infection
WO2007130655A3 (en) Phage derived antimicrobial activities
WO2005011592A3 (en) Substituted indazole-o-glucosides
AU2003291227A1 (en) Antibacterial agents
HK1083511A1 (en) Antibacterial amide macrocycles
WO2006003492A3 (en) Compositions and methods for treating pathological infections
WO2006081289A3 (en) Antibacterial agents
WO2006081182A3 (en) Antibacterial agents
TW200630367A (en) Substituted adenines and the uses thereof
WO2008133734A3 (en) Method and compositions for treating hiv infections
WO2006081178A3 (en) Antibacterial agents
WO2006128089A3 (en) Compositions and methods for treating tissue
EP1560821B8 (en) Antibacterial agents
WO2006050826A3 (en) Treatment of mastitis with enrofloxacin
WO2006029398A8 (en) All-trans-retinol : all-trans-13,14-dihydroretinol saturase and methods of its use
WO2004041158A3 (en) Rifalazil compositions and therapeutic regimens
WO2005118613A3 (en) Antibacterial amide macrocycles

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

122 Ep: pct application non-entry in european phase