WO2005100610A3 - Virus detection method, primers therefor and screening kit - Google Patents

Virus detection method, primers therefor and screening kit Download PDF

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Publication number
WO2005100610A3
WO2005100610A3 PCT/GB2005/001366 GB2005001366W WO2005100610A3 WO 2005100610 A3 WO2005100610 A3 WO 2005100610A3 GB 2005001366 W GB2005001366 W GB 2005001366W WO 2005100610 A3 WO2005100610 A3 WO 2005100610A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection method
virus detection
screening kit
primers therefor
primers
Prior art date
Application number
PCT/GB2005/001366
Other languages
French (fr)
Other versions
WO2005100610A2 (en
Inventor
Keith Hart
Original Assignee
Univ Cardiff
Keith Hart
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0408409A external-priority patent/GB0408409D0/en
Priority claimed from GB0410892A external-priority patent/GB0410892D0/en
Priority claimed from GB0423138A external-priority patent/GB0423138D0/en
Priority claimed from GB0425085A external-priority patent/GB0425085D0/en
Application filed by Univ Cardiff, Keith Hart filed Critical Univ Cardiff
Publication of WO2005100610A2 publication Critical patent/WO2005100610A2/en
Publication of WO2005100610A3 publication Critical patent/WO2005100610A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention relates to a method and means for detecting, typing and characterising the presence of viruses in a tissue sample and in particular the invention relates to a novel light-emitting self-probing amplicon that comprises a primer, probe and signalling system.
PCT/GB2005/001366 2004-04-15 2005-04-08 Virus detection method, primers therefor and screening kit WO2005100610A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB0408409.1 2004-04-15
GB0408409A GB0408409D0 (en) 2004-04-15 2004-04-15 Virus detection method, primers therefor and screening kit
GB0410892.4 2004-05-15
GB0410892A GB0410892D0 (en) 2004-05-15 2004-05-15 Virus detection method, primers therefor and screening kit
GB0423138A GB0423138D0 (en) 2004-10-19 2004-10-19 Virus detection method,primers therefor and screening kit
GB0423138.7 2004-10-19
GB0425085A GB0425085D0 (en) 2004-11-13 2004-11-13 Virus detecting method primers therefor and screening kit
GB0425085.8 2004-11-13

Publications (2)

Publication Number Publication Date
WO2005100610A2 WO2005100610A2 (en) 2005-10-27
WO2005100610A3 true WO2005100610A3 (en) 2006-06-15

Family

ID=34965851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/001366 WO2005100610A2 (en) 2004-04-15 2005-04-08 Virus detection method, primers therefor and screening kit

Country Status (1)

Country Link
WO (1) WO2005100610A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830869B (en) * 2015-04-30 2018-06-01 武汉凯德维斯生物技术有限公司 The gene loci and its application that HPV relevant with uterine neck carcinogenesis is integrated

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022626A1 (en) * 1994-02-21 1995-08-24 Stichting Researchfonds Pathologie Human papilloma virus detection in a nucleic acid amplification process using general primers
WO1998042825A1 (en) * 1997-03-24 1998-10-01 Case Western Reserve University A transgenic model of colon cancer and anal cancer
WO2002103050A2 (en) * 2001-06-14 2002-12-27 University Of Wales College Of Medicine Virus detection method, primers therefor and screening kit

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022626A1 (en) * 1994-02-21 1995-08-24 Stichting Researchfonds Pathologie Human papilloma virus detection in a nucleic acid amplification process using general primers
WO1998042825A1 (en) * 1997-03-24 1998-10-01 Case Western Reserve University A transgenic model of colon cancer and anal cancer
WO2002103050A2 (en) * 2001-06-14 2002-12-27 University Of Wales College Of Medicine Virus detection method, primers therefor and screening kit

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 11 January 1997 (1997-01-11), "Primer for amplifying HPV E6 protein coding region.", XP002353876, retrieved from EBI accession no. GSN:AAT31827 Database accession no. AAT31827 *
DATABASE Geneseq [online] 18 December 2003 (2003-12-18), "HPV mRNA detection RT-PCR primer, SEQ ID No 1.", XP002353877, retrieved from EBI accession no. GSN:ADC60857 Database accession no. ADC60857 *
DATABASE Geneseq [online] 19 September 2001 (2001-09-19), "Human cervical cancer marker nucleic acid 302.", XP002353873, retrieved from EBI accession no. GSN:AAH69028 Database accession no. AAH69028 *
DATABASE Geneseq [online] 9 April 2002 (2002-04-09), "HPV capture sequence probe DP-4.", XP002353875, retrieved from EBI accession no. GSN:ABK28835 Database accession no. ABK28835 *
DATABASE Geneseq [online] 9 January 2002 (2002-01-09), "HPV type 16 E2 DNA fragment #1.", XP002353874, retrieved from EBI accession no. GSN:AAI68517 Database accession no. AAI68517 *
HART KEITH W ET AL: "Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 39, no. 9, September 2001 (2001-09-01), pages 3204 - 3212, XP002250694, ISSN: 0095-1137 *
JORDENS J ZOE ET AL: "Amplification with molecular beacon primers and reverse line blotting for the detection and typing of human papillomaviruses.", JOURNAL OF VIROLOGICAL METHODS, vol. 89, no. 1-2, September 2000 (2000-09-01), pages 29 - 37, XP002250695, ISSN: 0166-0934 *
THELWELL N ET AL: "Mode of action and application of Scorpion primers to mutation detection", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 19, 1 October 2000 (2000-10-01), pages 3752 - 3761, XP002247004, ISSN: 0305-1048 *
WHITCOMBE D ET AL: "Detection of PCR products using self-probing amplicons and fluorescence", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 8, August 1999 (1999-08-01), pages 804 - 807, XP002226672, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2005100610A2 (en) 2005-10-27

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