WO2005095343A1 - Dpp-iv inhibitors - Google Patents

Dpp-iv inhibitors Download PDF

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Publication number
WO2005095343A1
WO2005095343A1 PCT/EP2005/002010 EP2005002010W WO2005095343A1 WO 2005095343 A1 WO2005095343 A1 WO 2005095343A1 EP 2005002010 W EP2005002010 W EP 2005002010W WO 2005095343 A1 WO2005095343 A1 WO 2005095343A1
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WIPO (PCT)
Prior art keywords
alkyl
group
independently selected
optionally substituted
compound according
Prior art date
Application number
PCT/EP2005/002010
Other languages
French (fr)
Inventor
Paul John Edwards
Achim Feurer
Silvia Cerezo-Galves
Victor Giulio Matassa
Sonja Nordhoff
Claudia Rosenbaum
Stephan Bulat
Original Assignee
Santhera Pharmaceuticals (Schweiz) Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Santhera Pharmaceuticals (Schweiz) Gmbh filed Critical Santhera Pharmaceuticals (Schweiz) Gmbh
Priority to AU2005229330A priority Critical patent/AU2005229330B2/en
Priority to RU2006131597/04A priority patent/RU2345067C2/en
Priority to MXPA06010097A priority patent/MXPA06010097A/en
Priority to US10/591,108 priority patent/US20070197522A1/en
Priority to JP2007501183A priority patent/JP2007526267A/en
Priority to EP05715551A priority patent/EP1758861A1/en
Priority to CA002558446A priority patent/CA2558446A1/en
Publication of WO2005095343A1 publication Critical patent/WO2005095343A1/en
Priority to NO20064032A priority patent/NO20064032L/en

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Definitions

  • the present invention relates to a novel class of dipeptidyl peptidase inhibitors, including pharmaceutically acceptable salts and prodrugs thereof, which are useful as therapeutic compounds, particularly in the treatment of Type 2 diabetes mellitus, often referred to as non-insulin dependent diabetes mellitus (NIDDM), and of conditions that are often associated with this disease, such as obesity and lipid disorders.
  • NIDDM non-insulin dependent diabetes mellitus
  • Diabetes refers to a disease process derived from multiple causative factors and characterized by elevated levels of plasma glucose or hyperglycemia in the fasting state or after administration of glucose during an oral glucose tolerance test. Persistent or uncontrolled hyperglycemia is associated with increased and premature morbidity and mortality. Often abnormal glucose homeostasis is associated both directly and indirectly with alterations of the lipid, lipoprotein and apolipoprotein metabolism and other metabolic and hemodynamic disease. Therefore patients with Type 2 diabetes mellitus are at an increased risk of macrovascular and microvascular complications, including coronary heart disease, stroke, peripheral vascular disease, hypertension, nephropathy, neuropathy, and retinopathy. Therefore, therapeutic control of glucose homeostasis, lipid metabolism and hypertension are critically important in the clinical management and treatment of diabetes mellitus.
  • Type 1 insulin-dependent, diabetes mellitus
  • IDDM insulin-dependent, diabetes mellitus
  • Type 2 noninsulin dependent, diabetes mellitus
  • NIDDM noninsulin dependent, diabetes mellitus
  • these patients develop a resistance to the insulin stimulating effect on glucose and lipid metabolism in the main insulin-sensitive tissues, namely the muscle, liver and adipose tissues. Further, the plasma insulin levels, while elevated, are insufficient to overcome the pronounced insulin resistance.
  • Insulin resistance is not primarily due to a diminished number of insulin receptors but to a post-insulin receptor binding defect that is not yet understood. This resistance to insulin responsiveness results in insufficient insulin activation of glucose uptake, oxidation and storage in muscle, and inadequate insulin repression of lipolysis in adipose tissue and of glucose production and secretion in the liver.
  • Type 2 diabetes which have not changed substantially in many years, have recognized limitations. While physical exercise and reductions in dietary intake of calories will dramatically improve the diabetic condition, compliance with this treatment is very poor because of well-entrenched sedentary lifestyles and excess food consumption, especially of foods containing high amounts of saturated fat.
  • sulfonylureas e.g., tolbutamide and glipizide
  • meglitinide which stimulate the pancreatic ⁇ -cells to secrete more insulin, and/or by injection of insulin when sulfonylureas or meglitinide become ineffective, can result in insulin concentrations high enough to stimulate the very insulin-resistant tissues.
  • sulfonylureas or meglitinide sulfonylureas or meglitinide
  • the biguanides increase insulin sensitivity resulting in some correction of hyperglycemia.
  • the two biguanides, phenformin and metformin can induce lactic acidosis and nausea/diarrhoea.
  • Metformin has fewer side effects than phenformin and is often prescribed for the treatment of Type 2 diabetes.
  • the glitazones are a recently described class of compounds with potential for ameliorating many symptoms of Type 2 diabetes. These agents substantially increase insulin sensitivity in muscle, liver and adipose tissue in several animal models of Type 2 diabetes, resulting in partial or complete correction of the elevated plasma levels of glucose without occurrence of hypoglycemia.
  • the glitazones that are currently marketed are agonists of the peroxisome proliferator activated receptor (PPAR), primarily the PPAR-gamma subtype.
  • PPAR-gamma agonism is generally believed to be responsible for the improved insulin sensitization that is observed with the glitazones.
  • Newer PPAR agonists that are being tested for treatment of Type 2 diabetes are agonists of the alpha, gamma or delta subtype, or a combination of these, and in many cases are chemically different from the glitazones (i.e., they are not thiazolidinediones). Serious side effects (e.g., liver toxicity) have occurred with some of the glitazones, such as troglitazone.
  • alpha-glucosidase inhibitors e.g., acarbose
  • PTP-1B protein tyrosine phosphatase-IB
  • DPP-IV dipeptidyl peptidase-IV
  • WO-A-97/40832 WO-A-98/19998
  • WO-A-03/180 WO-A-03/181.
  • the usefulness of DPP-IV inhibitors in the treatment of Type 2 diabetes is based on the fact that DPP-IV in vivo readily inactivates glucagon like peptide-1 (GLP-1 ) and gastric inhibitory peptide (GIP).
  • GLP-1 and GIP are incretins and are produced when food is consumed. The incretins stimulate production of insulin.
  • DPP-IV inhibition leads to decreased inactivation of the incretins, and this in turn results in increased effectiveness of the incretins in stimulating production of insulin by the pancreas. DPP-IV inhibition therefore results in an increased level of serum insulin.
  • DPP-IV inhibition is not expected to increase the level of insulin at , inappropriate times, such- as between meals, which can lead to excessively low blood sugar (hypoglycemia). Inhibition of DPP-IV is therefore expected to increase insulin without increasing the ' risk of hypoglycemia, which is a dangerous side effect associated with the use of insulin secretagogues.
  • DPP-IV inhibitors may also have other therapeutic utilities, as discussed elsewhere in this application.
  • DPP-IV inhibitors have not been studied extensively to date, especially for utilities other than diabetes. New compounds are needed so that improved DPP-IV inhibitors can be found for the treatment of diabetes and potentially other diseases and conditions.
  • the object of the present invention is to provide a new class of DPP-IV inhibitors which may be effective in the treatment of Type 2 diabetes and other DPP-IV modulated diseases.
  • R 9 is selected from the group consisting of C ⁇ alkyl; O-C ⁇ alkyl; and S-C ⁇ alkyl, wherein R 9 is optionally interrupted by oxygen and wherein R 9 is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 1 , R 4 are independently selected from the group consisting of H; F; OH; and R 4a ;
  • R 2 , R 5 are independently selected from the group consisting of H; F; and R 4 ;
  • R 4a is independently selected from the group consisting of C ⁇ - 6 alkyl; and 0-C ⁇ - 6 alkyl, wherein R 4a is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 4b is C- ⁇ - 6 alkyl, wherein R 4b is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 3 is selected from the group consisting of H; and C-j- ⁇ alkyl;
  • R 1 , R 2 , R 3 , R 4 , R 5 independently selected from the group consisting of R /R 2 ; R 2 /R 3 ; R 3 /R 4 ; and R 4 /R 5 form a C 3 - 7 cycloalkyl ring, which is optionally substituted with one or more of R 12 , wherein R 12 is independently selected from the group consisting of F; Cl; and OH; X is selected from the group consisting of S(O); S(O) 2 ; C(O); and C(R 13 R 14 );
  • R 13 , R 14 are independently selected from the group consisting of H; F; d-e alkyl; R 15 ; and R 16 ;
  • R 5 , R 13 , R 14 selected from the group consisting of R 5 /R 13 ; and R 13 /R 14 form a C 3 - 7 cycloalkyl ring, which is optionally substituted with one or more R 17 , wherein R 17 is independently selected from the group consisting of F; Cl; and OH;
  • R 15 is selected from the group consisting of phenyl; naphthyl; and indenyl, wherein R 15 is optionally substituted with one or more R 18 , wherein R 18 is independently selected from the group consisting of R 19 ; R 20 ; halogen; CN; COOH; OH; C(0)NH 2 ; S(0) 2 NH 2 ; S(0)NH 2 ; d-e alkyl; O-d-e alkyl; S-C ⁇ - 6 alkyl; COO-C ⁇ - 6 alkyl; OC(0)-C ⁇ - 6 alkyl C(0)N(R 21 )-C 1 - 6 alkyl; S(0) 2 N(R 21 )-C 1 - 6 alkyl; S(0)N(R 21 )-C 1 - 6 alkyl; S(0) 2 -C ⁇ - 6 alkyl S(0)-C 1 - 6 alkyl; N(R 21 )S(0) 2 -C 1 - 6 alkyl; and. N(R 21 )
  • R 19 is selected from the group consisting of phenyl; and naphthyl, wherein R 19 is optionally substituted with one or more R 24 , wherein R 24 is independently selected from the group consisting of halogen; CN; COOH; OH; C(0)NH 2 ; S(0) 2 NH 2 ; S(0)NH 2 ; d- 6 alkyl; 0-C 1 - 6 alkyl; S-d-e alkyl; COO-d- 6 alkyl; OC(0)-d- 6 alkyl; C(0)N(R 25 )-d- 6 alkyl; S(0) 2 N(R 25 )-d.
  • Ci-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 21 , R 23 , R 25 , R 27 are independently selected from the group consisting of H; and d- 6 alkyl, which is optionally substituted with one or more of R 28 , wherein R 28 is independently selected frorri the group consisting of F; Cl and OH;
  • R 6 , R 7 are independently selected from the group consisting of H; (C(R 29 R 30 )) m -X 1 -Z 1 ; and (C(R 31 R 32 )) n -X 2 -X 3 -Z 2 , provided that R 6 , R 7 are selected so that not both of R 6 , R 7 are independently selected from the group consisting of H; CH 3 ; CH 2 CH 3 ; CH 2 CH 2 CH 3 ; and CH(CH 3 ) 2 ;
  • R 6 , R 7 are independently C- ⁇ - alkyl, which is substituted with one or more R 29a , wherein R 29a is independently selected from the group consisting of R 29b ; and Z 1 , provided that R 6 , R 7 are selected so that not both of R 6 , R 7 are independently selected from the group consisting of CH 3 ; CH 2 CH 3 ; CH 2 CH 2 CH 3 ; and CH(CH 3 ) 2 ;
  • R 29 , R 29 , R 30 , R 31 , R 32 are independently selected from the group consisting of H; halogen; CN; OH; NH 2 ; COOH; C(0)NH 2 ; S(0) 2 NH 2 ; S(0)NH 2 ; d-e alkyl; 0-d- 6 alkyl; N(R 32a )-d- 6 alkyl; COO-d- 6 alkyl; OC(0)-d- 6 alkyl; C(0)N(R 32a )- d- 6 alkyl; N(R 32a )-C(O)-d- 6 alkyl; S(0) 2 N(R 32a )-d-e alkyl; S(O)N(R 32a )-d.
  • each d- 6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 32a is selected from the group consisting of H; and C ⁇ - 6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 29 , R 30 , R 31 , R 32 independently selected from the group consisting of R 29 /R 30 ; and R 31 /R 32 form a C 3 - 7 cycloalkyl ring, which is optionally substituted with one or more R 32 , wherein R 32b is independently selected from the group consisting of F; Cl; and OH;
  • n O, 1 , 2, 3 or 4;
  • n 2, 3 or 4;
  • X 1 is independently selected from the group consisting of a covalent bond -d- 6 travers alkyl-; -d- 6 alkyl-O-; -d-e alkyl-N(R 33 )-; -C(O)-; -C(0)-d-e alkyl- -C(0) d- 6 alkyl-O-; -C(0)-d- 6 alkyl-N(R 33 )-; -C(0)0-; -C(0)0-d- 6 alkyl- -C(0)0-d- 6 alkyl-O-; -C(0)0-d- 6 alkyl-N(R 33 )-; -C(0)N(R 33 )-; -C(0)N(R 33 )-; -C(0)N(R 33 )-d- 6 alkyl- -C(0)N(R 33 )-d- 6 alkyl-O-; -C(0)N(R 33 )-d- 6 alkyl-N(R 34 )-;
  • each d- 6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • X 2 is selected from the group consisting of -0-; -S-; -S(O)-; S(0) 2 -; and -N(R 35 )-;
  • X 3 is selected from the group consisting of a covalent bond; -C ⁇ . 6 alkyl-; -d- 6 alkyl-O- -d- ⁇ alkyl-N(R 36 )-; -C(O)-; -C(0)-d- 6 alkyl-; -C(0)-d- 6 alkyl-O-; -C(0)-d- 6 alkyl-N(R 36 )- -C(0)0-; -C(0)0-d-e alkyl-; -C(O)O-d- 6 alkyl-O-; -C(0)0-d-e alkyl-N(R 36 )- -C(0)N(R 36 )-; -C(0)N(R 36 )-C 1 - 6 alkyl-; -C(0)N(R 36 )-d- 6 alkyl-O-; and -C(0)N(R 36 )-d- 6 alkyl-N(R 37 )-; wherein each d-
  • X 2 -X 3 are independently selected from the group consisting of -N(R 35 )-S(0) 2 ; -N(R 35 )-S(0)-; -N(R 35 )-S(0) 2 -d- 6 alkyl-; -N(R 35 )-S(0)-d- 6 alkyl-; -N(R 35 )-S(0) 2 -d- 6 alkyl-O-; -N(R 35 )-S(0)-C 1 - 6 alkyl-O-; -N(R 35 )-S(0) 2 -d- 6 alkyl-N(R 36 )-; and -N(R 35 )-S(0)-d- 6 alkyl-N(R 36 )-; wherein each C- ⁇ - 6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • R 33 , R 34 , R 35 , R 36 , R 37 are independently selected from the group consisting of H; and Ci- 6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
  • Z 1 , Z 2 are independently selected from the group consisting of Z 3 ; and -C(R 37a )Z 3a Z 3b ;
  • R 37a is selected from the group consisting of H; and d- 6 alkyl, which is optionally substituted with one or more F;
  • Z 3 , Z 3a , Z 3b are independently selected from the group consisting of H; T 1 ; T 2 ; Ci- 6 alkyl; d- 6 alkyl-T 1 ; and C ⁇ - 6 alkyl-T 2 ; wherein each Ci-6 alkyl is optionally substituted with one or more R 37b , wherein R 37b is independently selected from the group consisting of halogen; CN; OH; NH 2 ; COOH; C(0)NH 2 ; S(0) 2 NH 2 ; S(0)NH 2 ; C ⁇ - 6 alkyl O-d-e alkyl; N(R 37c )-d- 6 alkyl; COO-d- 6 alkyl; OC(0)-d- 6 alkyl; C(0)N(R 37c )- d- 6 alkyl N(R 370 )-C(O)-C 1 - 6 alkyl; S(0) 2 N(R 37c )-d- 6 alkyl; S(0)N(R 37c
  • T 1 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T 1 is optionally substituted with one or more R 38 ; wherein R 38 is independently selected from the group consisting of halogen; CN; R 39 ; COOH; OH; C(O)NH 2 ; S(0) 2 NH 2 ; S(0)NH 2 ; COOT 3 ; OT 3 ; ST 3 ; C(O)N(R 40 )T 3 ; S(O) 2 N(R 40 )T 3 ; S(O)N(R 40 )T 3 and T 3 ;
  • R 45 is independently selected from the group consisting of F; COOR 46 ; C(0)N(R 46 R 47 ); S(0) 2 N(R 46 R 47 ); OR 46 ; N(R 46 R 47 ); T 3 ; O-T 3 ; and N(R 46 )-T 3 ;
  • R 42 is selected from the group consisting of C 1 -6 alkyl; 0-d- 6 alkyl; S-C 1 - 6 alkyl; N(R 48 )-d- 6 alkyl; COO-d- 6 alkyl; OC(O)- d- 6 alkyl; C(0)N(R 48 )- d- 6 alkyl; N(R 48 )-C(0)- d-e alkyl; S(0) 2 N(R 48 )-C 1 - 6 alkyl; S(O) N(R 48 )-d- 6 alkyl; S(0)-d- 6 alkyl; S(0) 2 -d- 6 alkyl; -N(R 48 )S(0) 2 -d- 6 alkyl; and -N(R 48 )S(0)-C 1 .
  • each d- 6 alkyl is optionally substituted with one or more R 45 , wherein R 45 is independently selected from the group consisting of F; COOR 49 ; C(0)N(R 49 R 50 ); S(0) 2 N(R 49 R 5 °); S(0)N(R 49 R 50 ); OR 49 ; N(R 49 R 50 ); T 3 ; O-T 3 ; and N(R 49 )-T 3 ;
  • R 40 , R 43 , R 44 , R 46 , R 47 , ' R 4 ?, R 49 , R 50 are independently selected from the group consisting of H; and d- 6 alkyl;
  • T 3 is selected from the group consisting of T 4 ; and T 5 ;
  • T 4 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T 4 is optionally substituted with one or more R 51 , wherein R 51 is independently selected from the group consisting of halogen; CN; COOR 52 ; OR 52 ; C(0)N(R 52 R 53 ); S(0) 2 N(R 52 R 53 ); Ci- 6 alkyl; O-C 1 -6 alkyl; S-C ⁇ - 6 alkyl; COO-d- 6 alkyl; OC(0)-C ⁇ .
  • each -e alkyl is optionally substituted with one more halogen selected from the group consisting of F; and Cl;
  • R 52 , R 53 , R 55 , R 56 are independently selected from the group consisting of H; and C 1 -6 alkyl.
  • variable or substituent can be selected from a group of different variants and such variable or substituent occurs more than once the respective variants can be the same or different.
  • Alkyl means a straight-chain or branched carbon chain that may contain double or triple bonds. It is generally preferred that alkyl doesn't contain double or triple bonds.
  • C ⁇ - 4 Alkyl means an alkyl chain having 1 - 4 carbon atoms, e.g.
  • -CH 2 -, -CH 2 -CH 2 -, -CH CH-, -CH(CH 3 )-, -C(CH 2 )-, -CH 2 -CH 2 -CH 2 -, - CH(C 2 H 5 )-, -CH(CH 3 ) 2 -.
  • -CH 2 -, -CH 2 -CH 2 -, -CH CH-, -CH(CH 3 )-, -C(CH 2 )-, -CH 2 -CH 2 -CH 2 -, -CH(C 2 H 5 )-, -CH(CH 3 ) 2 -.
  • Each hydrogen of a C ⁇ - 6 alkyl carbon may be replaced by a substituent.
  • Cycloalkyl or “C 3 - 7 Cycloalkyl ring” means a cyclic alkyl chain having 3 - 7 carbon atoms, e.g. cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl. Each hydrogen of a cycloalkyl carbon may be replaced by a substituent.
  • Halogen means fluoro, chloro, bromo or iodo. It is generally preferred that halogen is fluoro or chloro.
  • Examples for a heterocycle are furan, thiophene, pyrrole, pyrroline, imidazole, imidazoline, pyrazole, pyrazoline, oxazole, oxazoline, isoxazole, isoxazoline, thiazole, thiazoline, isothiazole, isothiazoline, thiadiazole, thiadiazoline, tetrahydrofuran, tetrahydrothiophene, pyrrolidine, imidazolidine, pyrazolidine, oxazolidine, isoxazolidine, thiazolidine, isothiazolidine, thiadiazolidine, sulfolane, pyran, dihydropyran, tetrahydropyran, imidazolidine, pyridine, pyridazine, pyrazine, pyrimidine, piperazine, piperidine, morpholine, tetrazole, triazole, tri
  • Heterobicycle means a heterocycle which is condensed with phenyl or an additional heterocycle to form a bicyclic ring system.
  • Condensed to form a bicyclic ring means that two rings are attached to each other by sharing two ring atoms.
  • heterobicycle examples include indole, indoline, benzofuran, benzothiophene, benzoxazole, benzisoxazole, benzothiazole, benzisothiazole, benzimidazole, benzimidazoline, quinoline, quinazoline, dihydroquinazoline, dihydroquinoline, isoquinoline, tetrahydroisoquinoline, dihydroisoquinoiine, benzazepine, purine or pteridine.
  • Preferred compounds of formula (I) or (la) are those compounds in which one or more of the residues contained therein have the meanings given below, with all combinations of preferred substituent definitions being a subject of the present invention. With respect to all preferred compounds of the formulas (I) or (la) the present invention also includes all tautomeric and stereoisomeric forms and mixtures thereof in all ratios, and their pharmaceutically acceptable salts.
  • the substituents Z, R 1 -R 7 and X of the formula (I) or (la) independently from each other have the following meaning.
  • one or more of the substituents Z, R 1 -R 7 and X can have the preferred or more preferred meanings given below.
  • Z is as defined above.
  • Z is phenyl or heterocycle.
  • Z is preferably an aromatic heterocycle.
  • Z is optionally substituted with 1 , 2 or 3, in one embodiment 1 or 2, R 8 , which are the same or different. Preferably, they are the same.
  • R 8 is as defined above.
  • R 8 is selected from the group consisting of Cl; F; CN; CH 3 ; and OCH 3 , more preferably Cl, F or CN, most preferably F.
  • Z is 2-Fluoro-phenyl. In another preferred embodiment Z is 2,4,5-Trifluoro-phenyl.
  • R 1 , R 4 are independently as defined above.
  • R 1 , R 4 are independently selected from the group consisting of H; F; OH; CH 3 ; and OCH 3 , more preferably H or F.
  • R 2 , R 5 are independently as defined above.
  • R 2 , R 5 are independently selected from the group consisting of H; F; and CH 3 , more preferably H or F.
  • R 1 , R 2 , R 4 , R 5 are more preferred H.
  • R 3 is as defined above.
  • R 3 is H.
  • X is as defined above.
  • X is C(O).
  • X is preferably S(O) 2 .
  • R 6 is as defined above.
  • R 6 is preferably selected from the group consisting of H, d- alkyl and C 3 - 7 cycloalkyl, more preferably H, CH 3 and cyclopropyl, most preferably H and CH 3 .
  • R 6 is preferably CH(R 29a ) 2 ; CHR 29a -CH 2 R 29a ; CH 2 -CH(R 29a ) 2 ; CH 2 - CHR 29a -CH 2 R 29a ; and CH 2 -CH 2 -CH(R 29a ) 2 .
  • both R 9a are preferably different, wherein one R 29a is R 29b and the other R 29a is Z 1 ; wherein Z 1 is preferably selected from T 2 .
  • Preferred embodiments for R 29b and T 2 are as set forth below.
  • T 2 is 5- or 6-membered heterocycle, preferably a saturated aromatic heterocycle containing preferably at least one N and optionally an O.
  • Z 2 is pyridyl or morpholinyl.
  • X I is as.defined above: Preferably, X 1 is a covalent bond.
  • n is as defined above.
  • m is 0, 1 , 2 or 3, more preferably 0 or 1.
  • R 7 is as defined above.
  • R 7 is Z 1 .
  • Z 1 is preferably T 2 , wherein T 2 is as defined above, preferably selected from the group consisting of C 3 . 7 cycloalkyl; indanyl; tetralinyl; decalinyl; and heterobicycle, more preferably indanyl; tetralinyl; and decalinyl; wherein T 2 is optionally substituted with one or more R 4 . It is preferred that T 2 is unsubstituted or substituted with one R 41 .
  • R 4 is as defined above, preferably halogen, methyl, O-methyl or OH, most preferred is OH.
  • R 7 is C ⁇ - alkyl, substituted with 1-4, i.e. 1 , 2, 3 or 4, preferably 1 or 2, R 29a , which are the same or different.
  • R 7 is more preferred selected from the group consisting of CH(R 29a ) 2 ; CHR 29a -CH 2 R 29a ; CH 2 -CH(R 29a ) 2 ; CH 2 -CHR 29a -CH 2 R 29a ; and CH 2 -CH 2 -CH(R 29a ) 2 .
  • R 7 is selected from the group consisting of CHR 29a -CH(CH 3 ) 2 .
  • R 7 is (C(R 31 R 3 )) n -X 2 -X 3 -Z 2 .
  • R 31 and R 32 are preferably both H. Integer n is preferably 1 or 2, more preferably 1.
  • X 2 is preferably NH or O, more preferably O.
  • X 3 is preferably a covalent bond, CH 2j or C(O), more preferably C(O).
  • Z 2 is preferably T 2 , more preferably a 5- or 6-membered heterocycle, preferably an aromatic heterocycle containing preferably at least one N. Most preferably Z 2 is pyridyl.
  • R 29a is as defined above.
  • R 29a is selected from the group consisting of R 29b ; and Z 1 ; wherein R 29b is preferably selected from the group consisting of H, halogen, N(R 32a )-d- 6 alkyl, NH 2 , and d- 6 alkyl, more preferably H; F; Cl; NH 2 ; NHCH 3 ; N(CH 3 ) 2 ; CH 3 ; and C 2 H 5 .
  • R 29b is selected from the group consisting of H; N(CH 3 ) 2 and CH 3 .
  • R 29a is selected from the group consisting of R 29b ; and Z 1 ; wherein Z 1 is selected from the group consisting of T 1 ; and T 2 .
  • two R 29a are present and both R 29a are preferably different, wherein one R 29a is R 29b and the other R 29a is Z 1 ; wherein Z is preferably selected from the group consisting of T and T 2 .
  • R 29a are present and both R 29a are preferably the same or different, more preferably different, and are Z 1 ; wherein Z 1 is selected from the group consisting of T 1 and T 2 .
  • R 7 is selected from the group consisting of CH(R 9a ) 2 ; CHR 29a -CH 2 R 29a ; CH 2 -CHR 29a -CH 2 R 29a ; and CH 2 -CH 2 - CH(R 29a ) 2 , more preferably CH 2 -CH(R 29a ) 2 and CH 2 -CHR 29a -CH 2 R 29a .
  • both of R 29a are selected from T 1 ; or that one R 29a is selected from T 1 and the other one R 29a is selected from T 2 .
  • T 1 is selected from both of R 29a ; or that one R 29a is selected from T 1 and the other one R 29a is selected from T 2 .
  • CH 2 -CHR 29a -CH 2 R 29a CH 2 -CHT 1 -CH 2 T 2 is preferred.
  • T 1 is as defined above.
  • T 1 is phenyl; wherein T 1 is optionally substituted with 1-3, i.e. 1 , 2 or 3, more preferably 1 or 2, most preferably 1 , R 38 , which are the same or different.
  • R 38 is as defined above.
  • R 38 is independently selected from the group consisting of halogen, CN, S0 2 NH 2 , CONH 2 , C ⁇ - 6 alkyl optionally substituted with 1 , 2 or 3 R 45 ; 0-d- 6 alkyl optionally substituted with 1 R 45 ; S-d- 6 alkyl; S(0)-d_ 6 alkyl; S(O)- d-6 alkyl; S(0) 2 -d- 6 alkyl; CONHT 3 ; CONH-d- 6 alkyl optionally substituted with 1 R 45 ; T 3 ; and O-T 3 .
  • R 38 is independently selected from the group consisting of halogen; CN; S0 2 NH 2 ; CONH 2 ;
  • 0-d- 6 alkyl optionally substituted with 1 or 2 F or N(R 49 R 50 );
  • R 51 phenyl optionally substituted with 1 or 2 R 51 ; heterocyclyl;
  • O-phenyl optionally substituted with 1 or 2 R 5 .
  • R 38 is independently selected from the group consisting of F;
  • O-phenyl optionally substituted with 1 R 51 .
  • R 38 is independently selected from the group consisting of F; Cl; CN; CH 3 ; C 2 H 5 ; CH 2 CH 2 CH 3 ; CH(CH 3 ) 2 ; CF 3 ; 0-CH 3 ; 0-C 2 H 5 ; S-CH 3 ; S0 2 NH 2 ; T 3 ; and O-T 3 .
  • T 1 is preferably phenyl; wherein T 1 is unsubstituted or substituted with 1-3, i.e. 1 , 2 or 3, more preferably 1 or 2, most preferably 1 , R 38 selected from F; Cl; CN; CH 3 ; C 2 H 5 ; CH 2 CH 2 CH 3 ; CH(CH 3 ) 2 ; CF 3 ; O- CH 3 ; 0-C 2 H 5 ; S-CH 3 ; S0 2 NH 2 ; T 3 ; and O-T 3 , more preferably F; Cl; CN; CH 3 , most preferably F or Cl.
  • T 2 is as defined above.
  • T 2 is selected from the group consisting of indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle; more preferably
  • heterocycle preferably a 5- or 6-membered, heterocycle containing at least one heteroatom, preferably N or O
  • the heterocycle can be selected from the group of (a) aromatic, preferably 5- or 6-membered ring and preferably containing at least one heteroatom selected from N, more preferably containing 1 or 2 N and optionally an O, most preferably selected from the group
  • T is selected from the group consisting of
  • T 2 is preferably a N- containing heterocycle, preferably containing 4, 5 or 6 ring members and optionally containing a further heteroatom selected from N or O. Most preferably T 2 is selected from the group
  • T 2 is optionally substituted with 1 , 2 or 3, preferably 1-2 R 41 , which are the same or different.
  • R 41 is independently selected from the group consisting of halogen; CN; OH; C ⁇ - 6 alkyl optionally substituted with 1 , 2 or 3 F; O-C-i-e alkyl substituted with 1 , 2 or 3 F; NH-C(0)-d- 6 alkyl; and T 3 .
  • R 4 is selected from the group consisting of
  • T 4 optionally substituted with 1 or 2, more preferably 1 , R 51 , whereby R 51 is as defined herein; or
  • R 41 is selected from the group consisting of
  • NH-C(0)-d- 2 alkyl phenyl optionally substituted with F; Cl; CH 3 ; C 2 H 5 ; 0-CH 3 ; or 0-C 2 H 5l or cyclopropyl.
  • R 41 is as defined above.
  • R 41 is selected from the group consisting of OH; CH 3 ; and T 3 ;
  • T 3 is as defined above.
  • T 3 is T 4 .
  • T 4 is as defined above.
  • T 4 is phenyl, wherein T 4 is optionally substituted with 1-3 R 5 , which are the same or different.
  • R 51 is as defined above.
  • R 51 is independently selected from the group consisting of F; Cl; CH 3 ; C 2 H 5 ; CH 2 CH 2 CH 3 ; CH(CH 3 ) 2 ; CF 3 ; 0-CH 3 ; 0-C 2 H 5 ; S-CH 3 ; and S0 2 NH 2 .
  • T 3 i is T T5 T 5 is as defined above.
  • T 5 is heterocycle, more preferably a 5- or 6- membered heterocycle.
  • the heterocycle may be saturated or aromatic.
  • the heterocycle may contain at least one N.
  • Preferred examples of the heterocycle include:
  • T is optionally substituted with 1-2 R , which are the same or different.
  • R 54 is as defined above.
  • R 54 is selected from the group consisting of OH; and CH 3 .
  • the present invention provides prodrug compounds of the compounds of the invention as described above.
  • Prodrug compound means a derivative that is converted into a compound according to the present invention by a reaction with an enzyme, gastric acid or the like under a physiological condition in the living body, e.g. by oxidation, reduction, hydrolysis or the like, each of which is carried out enzymatically.
  • Examples of the prodrug are compounds, wherein the amino group in a compound of the present invention is acylated, alkylated or phosphorylated to form, e.g., eicosanoylamino, alanylamino, pivaloyloxymethylamino or wherein the hydroxyl group is acylated, alkylated, phosphorylated or converted into the borate, e.g.
  • Metabolites of compounds of formula (1) or (la) are also within the scope of the present invention.
  • tautomerism like e.g. keto-enol tautomerism, of compounds of general formula (I) or (la) or their prodrugs
  • the individual forms like e.g. the keto and enol form, are claimed separately and together as mixtures in any ratio.
  • stereoisomers like e.g. enantiomers, cis/trans isomers, conformers and the like.
  • isomers can be separated by methods well known in the art, e.g. by liquid chromatography.
  • enantiomers by using e.g. chiral stationary phases.
  • enantiomers may be isolated by converting them into diastereomers, i.e.
  • any enantiomer of a compound of formula (I) or (la) may be obtained from stereoselective synthesis using optically pure starting materials.
  • the invention also comprises their corresponding pharmaceutically or toxicologically acceptable salts, in particular their pharmaceutically utilizable salts.
  • the compounds of the formula (I) or (la) which contain acidic groups can be present on these groups and can be used according to the invention, for example, as alkali metal salts, alkaline earth metal salts or as ammonium salts.
  • salts include sodium salts, potassium salts, calcium salts, magnesium salts or salts with ammonia or organic amines such as, for example, ethylamine, ethanolamine, triethanolamine or amino acids.
  • Compounds of the formula (I) or (la) which contain one or more basic groups, i.e. groups which can be protonated, can be present and can be used according to the invention in the form of their addition salts with inorganic or organic acids.
  • acids examples include hydrogen chloride, hydrogen bromide, phosphoric acid, sulfuric acid, nitric acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisulfonic acids, oxalic acid, acetic acid, tartaric acid, lactic acid, salicylic acid, benzoic acid, formic acid, propionic acid, pivalic acid, diethylacetic acid, malonic acid, succinic acid, pimelic acid, fumaric acid, maleic acid, malic acid, sulfaminic acid, phenylpropionic acid, gluconic acid, ascorbic acid, isonicotinic acid, citric acid, adipic acid, and other acids known to the person skilled in the art.
  • the invention also includes, in addition to the salt forms mentioned, inner salts or betaines (zwitterions).
  • the respective salts according to the formula (1) or (la) can be obtained by customary methods which are known to the person skilled in the art like, for example by contacting these with an organic or inorganic acid or base in a solvent or dispersant, or by anion exchange or cation exchange with other salts.
  • the present invention also includes all salts of the compounds of the formula (I) or (la) which, owing to low physiological compatibility, are not directly suitable for use in pharmaceuticals but which can be used, for example, as intermediates for chemical reactions or for the preparation of pharmaceutically acceptable salts.
  • DPP-IV is a cell surface protein that has been implicated in a wide range of biological functions. It has a broad tissue distribution (intestine, kidney, liver, pancreas, placenta, thymus, spleen, epithelial cells, vascular endothelium, lymphoid and myeloid cells, serum), and distinct tissue and cell-type expression levels. DPP-IV is identical to the T cell activation marker CD26, and it can cleave a number of immunoregulatory, endocrine, and neurological peptides in vitro. This has suggested a potential role for this peptidase in a variety of disease processes.
  • the present invention provides compounds of formula (I) or (la) or their prodrugs or pharmaceutically acceptable salt thereof for use as a medicament.
  • the present invention provides the use of compounds of formula (I) or (la) or their prodrugs or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for the treatment or prophylaxis of non-insulin dependent (Type II) diabetes mellitus; hyperglycemia; obesity; insulin resistance; lipid disorders; dyslipidemia; hyperlipidemia; hypertriglyceridemia; hypercholestrerolemia; low HDL; high LDL; atherosclerosis; growth hormone deficiency; diseases related to the immune response; HIV infection; neutropenia; neuronal disorders; tumor metastasis; benign prostatic hypertrophy; gingivitis; hypertension; osteoporosis; diseases related to sperm motility; low glucose tolerance; insulin resistance; ist sequelae; vascular restenosis; irritable bowel syndrome; inflammatory bowel disease; including Crohn's disease and ulcerative colitis; other inflammatory conditions; pancreatitis; abdominal obesity; neurodegenerative disease; anxiety; depression; retinopathy; nephropathy;
  • the present invention provides pharmaceutical compositions comprising a compound of formula (I) or (la), or a prodrug compound thereof, or a pharmaceutically acceptable salt thereof as active ingredient together with a pharmaceutically acceptable carrier.
  • “Pharmaceutical composition” means one or more active ingredients, and one or more inert ingredients that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutically acceptable carrier.
  • a pharmaceutical composition of the present invention may additionally comprise one or more other compounds as active ingredients like one or more additional compounds of formula (I) or (la), or a prodrug compound or other DPP-IV inhibitors.
  • Other active ingredients are disclosed in WO-A-03/181 under the paragraph "Combination Therapy” which is herewith incorporated by reference.
  • other active ingredients may be insulin sensitizers; PPAR agonists; biguanides; protein tyrosinephosphatase-lB (PTP-1 B) inhibitors; insulin and insulin mimetics; sulfonylureas and other insulin secretagogues; a-glucosidase inhibitors; glucagon receptor antagonists; GLP-1 , GLP-1 mimetics, and GLP-1 receptor agonists; GIP, GIP mimetics, and GIP receptor agonists; PACAP, PACAP mimetics, and PACAP receptor 3 agonists; cholesterol lowering agents; HMG-CoA reductase inhibitors; sequestrants; nicotinyl alcohol; nicotinic acid or a salt thereof; PPARa agonists; PPARoly dual agonists; inhibitors of cholesterol absorption; aeyl CoA : cholesterol acyltransferase inhibitors; anti-oxidants; PPARo agonists; antio
  • pharmaceutically acceptable salts refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids, including inorganic bases or acids and organic bases or acids.
  • the compounds of formula (I) or (la) can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques.
  • the carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous).
  • any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like in the case of oral liquid preparations, such as, for example, suspensions, elixirs and solutions; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like in the case of oral solid preparations such as, for example, powders, hard and soft capsules and tablets, with the solid oral preparations being preferred over the liquid preparations.
  • oral liquid preparations such as, for example, suspensions, elixirs and solutions
  • carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like in the case of oral solid preparations such as, for example, powders, hard and soft capsules and tablets, with the solid oral preparations being preferred over the liquid preparation
  • tablets and capsules represent the most advantageous oral dosage unit form in which case solid pharmaceutical carriers are obviously employed.
  • tablets may be coated by standard aqueous or nonaqueous techniques.
  • Such compositions and preparations should contain at least 0.1 percent of active compound.
  • the percentage of active compound in these compositions may, of course, be varied' arid may conveniently be between about 2 percent to about 60 percent of the weight of the unit.
  • the amount of active compound in such therapeutically useful compositions is such that an effective dosage will be obtained.
  • the active compounds can also be administered intranasally as, for example, liquid drops or spray.
  • the tablets, pills, capsules, and the like may also contain a binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin.
  • a dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil.
  • tablets may be coated with shellac, sugar or both.
  • a syrup or elixir may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor.
  • Compounds of formula (I) or (la) may also be administered parenterally. Solutions or suspensions of these active compounds can be prepared in water suitably mixed with a surfactant such as hydroxy-propylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
  • the pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions.
  • the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
  • the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.
  • Any suitable route of administration may be employed for providing a mammal, ⁇ especially a human, with an effective dose of a compound of the present invention.
  • oral, rectal, topical, parenteral, ocular, pulmonary, nasal, and the like may be employed.
  • Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols, and the like.
  • compounds of formula (I) or (la) are administered orally.
  • the effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration, the condition being treated and the severity of the condition being treated. Such dosage may be ascertained readily by a person skilled in the art.
  • the compounds of the present invention are administered at a daily dosage of from about 0.1 milligram to about 100 milligram per kilogram of animal body weight, preferably given as a single daily dose or in divided doses two to six times a day, or in sustained release form.
  • the total daily dosage is from about 1.0 milligrams to about 1000 milligrams, preferably from about 1 milligrams to about 50 milligrams. In the case of a 70 kg adult human, the total daily dose will generally be from about 7 milligrams to about 350 milligrams. This dosage regimen may be adjusted to provide the optimal therapeutic response.
  • Preferred embodiments of compounds having formula (I) of the present invention can be prepared from beta amino acid intermediates such as those of formula (II)
  • Available starting materials may be amines having the formula (III), which may be purchased from commercially available sources such as ABCR, Array, Astatech, Sigma-Aldrich, Fluka, or be synthesized by one skilled in the art.
  • amines having the formula (III) may be purchased from commercially available sources such as ABCR, Array, Astatech, Sigma-Aldrich, Fluka, or be synthesized by one skilled in the art.
  • Common nucleophilic substitution reactions between compounds containing a suitable leaving group e.g. halogenide, mesylate, tosylate, epoxides
  • nucleophiles e.g. amines
  • the conversion of diverse functional groups may allow the synthesis of various amines, e.g.
  • 3-amino-4-(2,4,5-trifluoro-phenyl)-butyric acid may be synthesized by a variety of methods as reported in the patent applications WO 2004069162, WO 2004064778, WO 2004037169, WO 2004032836 and in the articles JACS, 126, 3048 (2004) and JACS, 126, 9918 (2004).
  • Analytical LC/MS was performed using: a) Reprosil- Pur ODS 3.5 ⁇ M, 1 x 60 mm column with a linear gradient acetonitrile in water (0.1% TFA) at a flow rate of 250 ⁇ L/min; b) XTerra MS C18, 3.5 ⁇ m, 2.1 * 100 mm, linear gradient with acetonitrile in water (0.1% HCOOH) at a flow rate of 250 ⁇ L/min; retention times are given in minutes. Methods are:
  • X C(O) by standard peptide coupling conditions.
  • EDC 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
  • HOBt 1 -hydroxybenzotriazole
  • a base triethylamine or diisopropylethylamine
  • HATU ⁇ /,/ ⁇ /', ⁇ /-tetramethyluronium hexafluorophosphate
  • HBTU 0-(7-benzotriazol-1-yl)- ⁇ /,A/, ⁇ /' ⁇ /-tetramethyluronium hexafluorophosphate
  • solvents such as methylene chloride, ⁇ /,N-dimethylformamide..or dimethyl
  • acidic media such as in solvents like methanol or dichloromethane containing acetic acid
  • the leaving group may be a tosylate, halide, triflate or mesylate reacting with the amine in solvents such as dichloromethane, acetonitrile or ⁇ /, ⁇ /-dimethylformamide, in the presence of a base such as triethylamine or 2,6-lutidine, for example.
  • Scheme H outlines a procedure for using the amines of formula (III) and sulphonic acid chlorides of formula (lib) to synthesize preferred compounds that are embodiments of the invention.
  • Scheme I outlines a procedure for using solid phase synthesis to synthesize preferred compounds that are embodiments of the invention.
  • Scheme J outlines a procedure for using the amines of formula (III) and acids of formula (lla) to synthesize preferred compounds that are embodiments of the invention.
  • the protective group may be removed with, for example, diethylamine in dichloromethane in the case of Fmoc or using acidic conditions (such as trifluoroacetic acid in dichloromethane or hydrochloric acid in dioxane) in the case of Boc, as described in Protective Groups in Organic Synthesis. 3 rd ed., Ed. Wiley-VCH, New York; 1999.
  • acidic conditions such as trifluoroacetic acid in dichloromethane or hydrochloric acid in dioxane
  • the evolved hydrogen chloride is absorbed through a drying tube containing silica gel or calcium chloride to a surface of aqueous sodium hydroxide. After cooling with ice, 2 N hydrochloric acid is added and a yellow precipitate is formed.
  • To hydrolyze the ketimine of 5-chloro-2-amino-a-chloroacetophenone the mixture is warmed at 80 °C under stirring, until the precipitate has dissolved (ca. 30 min).
  • the cooled mixture is extracted with chloromethane (three times) and the organic layer is washed with water, dried with sodium sulphate, and concentrated.
  • the neutral fraction obtained (1.00 g) is recrystallized to obtain the pure 5-chloro-2-amino- ⁇ -chloroacetophenone.
  • the mixture is stirred overnight at ambient temperature and diluted with ethyl acetate.
  • the organic phase is washed sequentially with 5 % citric acid, saturated aqueous sodium bicarbonate solution, and brine, dried over sodium sulphate and concentrated under vacuum to yield the title compound.
  • the title compound was prepared according the procedure from step 1 example 26.
  • Step 2 NaBH 4 , isobutyric acid, N ⁇ — y- ⁇ n J eLi ' : THF ⁇ V >—_ ⁇ ⁇ / VMN H 2 N
  • reaction is quenched by addition of 1 mL water and 2 mL 1 M HCl.
  • aqueous phase is extracted with dichloromethane, and the combined organic layers are washed with 3 % hydrochloric acid solution, saturated sodium bicarbonate solution, water and brine, dried with sodium sulphate, filtered and concentrated in vacuo.
  • the title compound was prepared according to the procedure from example 30.
  • Boc-(3-aminomethyl)-benzoic acid in 3 mL N,N- dimethylformamide is added 25 mg (0.13 mmol) 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride (EDC), 18 mg (0.13 mmol) 1 -hydroxybenzotriazole (HOBt) and 49 ⁇ L (0.29 mmol) diisopropylethylamine (DIEA) and after 10 min 8 mg (0.14 mmol) cyclopropylamine. The mixture is stirred overnight at ambient temperature and diluted with ethyl acetate.
  • EDC 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride
  • HOBt 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride
  • HOBt 1-ethyl-3-(3-dimethyla
  • step 2 the crude material from step 2 (0.10 mmol) is dissolved in 1 mL N,N- dimethylformamide and added to the reaction mixture. The mixture is stirred overnight at ambient temperature and diluted with ethyl acetate. The organic phase is washed sequentially with 5 % citric acid, saturated aqueous sodium bicarbonate solution and brine, dried over sodium sulphate and concentrated under vacuum. Purification by flash chromatography (silica gel, cyclohexane to ethyl acetate) affords the title compound. LCMS (Ilia) rt 2.65, m/z 492 (M+Na) + .
  • Example 37 prepared following the procedure above outlined for Example 36 according to Scheme J.
  • Example 38 prepared following the procedure outlined for Example 36 according to Scheme J.
  • Diastereoisomeric compounds generated from racemic amines are separated via reversed phase HPLC. The absolute configuration for these diastereoisomeres is not assigned.
  • Step 3 The resulting solution is stirred for 12 h at room temperature and the organic solvent is removed under reduced pressure. The residue is diluted with 10 mL of saturated sodium bicarbonate solution and extracted 3 times with ethyl acetate. The combined organic layers are washed with brine, dried with sodium sulphate and the solvent is evaporated. The crude product is used in step 3 without any further purification. Step 3
  • the resulting mixture is stirred for 1 h at 0°C, washed with water, dried with sodium sulphate, and the solvent is removed under reduced pressure.
  • the crude mesylate is dissolved in 10 mL ⁇ /, ⁇ /-dimethy] formamide and transfered into a flask, wrapped in aluminium foil, that had been charged with 0.89 g (2.73 mmol) cesium carbonate, 10 mL ⁇ /, ⁇ /-dimethyl formamide and 0.36 mL (5.03 mmol) thioacetic acid.
  • the reaction mixture is stirred for 12 h at room temperature. Afterwards the solution is poured into 50 mL of brine and the mixture is extracted 3 times with ethyl acetate.
  • Steps 1- 6 are carried out according to example 191.
  • Steps 1- 6 are carried out according to example 191. Step 7
  • DPP-IV peptidase activity was monitored with a continuous fluorimetric assay.
  • This assay is based on the cleavage of the substrate Gly-Pro-AMC (Bachem) by DPP-IV, releasing free AMC
  • the assay is carried out in 96-well microtiterplates. In a total volume of 100 ⁇ L, compounds are preincubated with 50 pM DPP-IV employing a buffer containing 10mM Hepes, 150mM NaCl, 0.005% Tween 20 (pH 7.4).
  • the reaction is started by the addition of 16 ⁇ M substrate and the fluorescence of liberated AMC is detected for 10 minutes at 25 °C with a fluorescence reader (BMG-Fluostar; BMG- , Technologies) using an excitation wavelength of 370 nm and an emission wavelength of 450 nm.
  • the final concentration of DMSO is 1 %.
  • the inhibitory potential of the compounds were determined.
  • DPP-IV activity assays were carried out with human and porcine DPP-IV (see below); both enzymes showed comparable activities.
  • Soluble human DPP-IV lacking the transmembrane anchor (Gly31-Pro766) was expressed in a recombinant YEAST-strain as Pre-Pro-alpha-mating fusion.
  • the secreted product (rhuDPP-IV-Gly31-Pro766) was purified from fermentation broth (>90% purity).
  • the 1C 50 values for inhibition of DPP-IV peptidase activity determined in assays as described above.
  • the IC 50 values were grouped in 3 classes: a ⁇ 100 nM; b >101 nM and ⁇ 1001 nM ; c >1001 nM ⁇ 2000 nM.

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Abstract

The invention relates to compounds of formula (I), Z-C(R<1>R<2>)-C(R<3>NH2)-C(R<4>R<5>)-X-N(R<6>R<7>), wherein Z, R<1-7> and X have the meaning as cited in the description and the claims. Said compounds are useful as DPP-lV inhibitors. The invention also relates to the preparation of such compounds as well as the production and use thereof as medicament.

Description

DPP-IV inhibitors
The present invention relates to a novel class of dipeptidyl peptidase inhibitors, including pharmaceutically acceptable salts and prodrugs thereof, which are useful as therapeutic compounds, particularly in the treatment of Type 2 diabetes mellitus, often referred to as non-insulin dependent diabetes mellitus (NIDDM), and of conditions that are often associated with this disease, such as obesity and lipid disorders.
Diabetes refers to a disease process derived from multiple causative factors and characterized by elevated levels of plasma glucose or hyperglycemia in the fasting state or after administration of glucose during an oral glucose tolerance test. Persistent or uncontrolled hyperglycemia is associated with increased and premature morbidity and mortality. Often abnormal glucose homeostasis is associated both directly and indirectly with alterations of the lipid, lipoprotein and apolipoprotein metabolism and other metabolic and hemodynamic disease. Therefore patients with Type 2 diabetes mellitus are at an increased risk of macrovascular and microvascular complications, including coronary heart disease, stroke, peripheral vascular disease, hypertension, nephropathy, neuropathy, and retinopathy. Therefore, therapeutic control of glucose homeostasis, lipid metabolism and hypertension are critically important in the clinical management and treatment of diabetes mellitus.
There are two generally recognized forms of diabetes. In Type 1 , or insulin-dependent, diabetes mellitus (IDDM), patients produce little or no insulin, which is the hormone regulating glucose utilization. In Type 2, or noninsulin dependent, diabetes mellitus (NIDDM), patients often have plasma insulin levels that are the same or elevated compared to nondiabetic subjects. These patients develop a resistance to the insulin stimulating effect on glucose and lipid metabolism in the main insulin-sensitive tissues, namely the muscle, liver and adipose tissues. Further, the plasma insulin levels, while elevated, are insufficient to overcome the pronounced insulin resistance.
Insulin resistance is not primarily due to a diminished number of insulin receptors but to a post-insulin receptor binding defect that is not yet understood. This resistance to insulin responsiveness results in insufficient insulin activation of glucose uptake, oxidation and storage in muscle, and inadequate insulin repression of lipolysis in adipose tissue and of glucose production and secretion in the liver.
The available treatments for Type 2 diabetes, which have not changed substantially in many years, have recognized limitations. While physical exercise and reductions in dietary intake of calories will dramatically improve the diabetic condition, compliance with this treatment is very poor because of well-entrenched sedentary lifestyles and excess food consumption, especially of foods containing high amounts of saturated fat. Increasing the plasma level of insulin by administration of sulfonylureas (e.g., tolbutamide and glipizide) or meglitinide, which stimulate the pancreatic β-cells to secrete more insulin, and/or by injection of insulin when sulfonylureas or meglitinide become ineffective, can result in insulin concentrations high enough to stimulate the very insulin-resistant tissues. However, dangerously low levels of plasma glucose can result from administration of insulin or insulin secretagogues (sulfonylureas or meglitinide), and an increased level of insulin resistance, due to the even higher plasma insulin levels, can occur. The biguanides increase insulin sensitivity resulting in some correction of hyperglycemia. However, the two biguanides, phenformin and metformin, can induce lactic acidosis and nausea/diarrhoea. Metformin has fewer side effects than phenformin and is often prescribed for the treatment of Type 2 diabetes.
The glitazones (i.e., 5-benzylthiazolidine-2,4-diones) are a recently described class of compounds with potential for ameliorating many symptoms of Type 2 diabetes. These agents substantially increase insulin sensitivity in muscle, liver and adipose tissue in several animal models of Type 2 diabetes, resulting in partial or complete correction of the elevated plasma levels of glucose without occurrence of hypoglycemia. The glitazones that are currently marketed are agonists of the peroxisome proliferator activated receptor (PPAR), primarily the PPAR-gamma subtype. PPAR-gamma agonism is generally believed to be responsible for the improved insulin sensitization that is observed with the glitazones. Newer PPAR agonists that are being tested for treatment of Type 2 diabetes are agonists of the alpha, gamma or delta subtype, or a combination of these, and in many cases are chemically different from the glitazones (i.e., they are not thiazolidinediones). Serious side effects (e.g., liver toxicity) have occurred with some of the glitazones, such as troglitazone.
Additional methods of treating the disease are still under investigation. New biochemical approaches that have been recently introduced or are still under development include treatment with alpha-glucosidase inhibitors (e.g., acarbose) and protein tyrosine phosphatase-IB (PTP-1B) inhibitors.
Compounds that are inhibitors of the dipeptidyl peptidase-IV (DPP-IV) enzyme are also under investigation as drugs that may be useful in the treatment of diabetes, and particularly Type 2 diabetes. See for example WO-A-97/40832, WO-A-98/19998, WO-A-03/180 and WO-A-03/181. The usefulness of DPP-IV inhibitors in the treatment of Type 2 diabetes is based on the fact that DPP-IV in vivo readily inactivates glucagon like peptide-1 (GLP-1 ) and gastric inhibitory peptide (GIP). GLP-1 and GIP are incretins and are produced when food is consumed. The incretins stimulate production of insulin. Inhibition of DPP-IV leads to decreased inactivation of the incretins, and this in turn results in increased effectiveness of the incretins in stimulating production of insulin by the pancreas. DPP-IV inhibition therefore results in an increased level of serum insulin. Advantageously, since the incretins are produced by the body only when food is consumed, DPP-IV inhibition is not expected to increase the level of insulin at , inappropriate times, such- as between meals, which can lead to excessively low blood sugar (hypoglycemia). Inhibition of DPP-IV is therefore expected to increase insulin without increasing the ' risk of hypoglycemia, which is a dangerous side effect associated with the use of insulin secretagogues.
DPP-IV inhibitors may also have other therapeutic utilities, as discussed elsewhere in this application. DPP-IV inhibitors have not been studied extensively to date, especially for utilities other than diabetes. New compounds are needed so that improved DPP-IV inhibitors can be found for the treatment of diabetes and potentially other diseases and conditions.
Thus, the object of the present invention is to provide a new class of DPP-IV inhibitors which may be effective in the treatment of Type 2 diabetes and other DPP-IV modulated diseases.
Accordingly, the present invention provides novel compounds of formula (I):
Z-C(R1R2)-C(R3NH2)-C(R4R5)-X-N(R6R7) (I), or a pharmaceutically acceptable salt thereof, wherein
Z is selected from the group consisting of phenyl; naphthyl; indenyl; C3-7 cycloalkyl; indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle, wherein Z is optionally substituted with one or more R8, wherein R8 is independently selected from the group consisting of halogen; CN; OH; NH2; oxo (=0), where the ring is at least partially saturated; R9; and R10;
R9 is selected from the group consisting of C^ alkyl; O-C^ alkyl; and S-C^ alkyl, wherein R9 is optionally interrupted by oxygen and wherein R9 is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R10 is selected from the group consisting of phenyl; heterocycle; and C3.7 cycloalkyl, wherein R10 is optionally substituted with one or more R1 , wherein R11 is independently selected from the group consisting of halogen; CN; OH; NH2; oxo (=O), where the ring is at least partially saturated; C-i-6 alkyl; 0-d-6 alkyl; and S-C-ι-6 alkyl; •
R1, R4 are independently selected from the group consisting of H; F; OH; and R4a;
R2, R5 are independently selected from the group consisting of H; F; and R4 ;
R4a is independently selected from the group consisting of Cι-6 alkyl; and 0-Cι-6 alkyl, wherein R4a is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R4b is C-ι-6 alkyl, wherein R4b is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R3 is selected from the group consisting of H; and C-j-β alkyl;
Optionally one or more pairs of R1, R2, R3, R4, R5 independently selected from the group consisting of R /R2; R2/R3; R3/R4; and R4/R5 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more of R12, wherein R12 is independently selected from the group consisting of F; Cl; and OH; X is selected from the group consisting of S(O); S(O)2; C(O); and C(R13R14);
R13, R14 are independently selected from the group consisting of H; F; d-e alkyl; R15; and R16;
Optionally one or both pairs of R5, R13, R14 selected from the group consisting of R5/R13; and R13/R14 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more R17, wherein R17 is independently selected from the group consisting of F; Cl; and OH;
R15 is selected from the group consisting of phenyl; naphthyl; and indenyl, wherein R15 is optionally substituted with one or more R18, wherein R18 is independently selected from the group consisting of R19; R20; halogen; CN; COOH; OH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; O-d-e alkyl; S-Cι-6 alkyl; COO-Cι-6 alkyl; OC(0)-Cι-6 alkyl C(0)N(R21)-C1-6 alkyl; S(0)2N(R21)-C1-6 alkyl; S(0)N(R21)-C1-6 alkyl; S(0)2-Cι-6 alkyl S(0)-C1-6 alkyl; N(R21)S(0)2-C1-6 alkyl; and. N(R21)S(0)-C1-6 alkyl, wherein each Ci-6 alkyl is optionally substituted with one1 or more halogen independently selected from the group consisting of F; and Cl;
R16 is selected from the group consisting of heterocycle; heterobicycle; C3-7 cycloalkyl; indanyl; tertralinyl; and decalinyl, wherein R16 is optionally substituted with one or more R22, wherein R22 is independently selected from the group consisting of R19; R20; halogen; CN; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; O-d-e alkyl; S-d-e alkyl; N(R23)-d-e alkyl; COO-d-e alkyl; OC(0)-d-e alkyl; C(0)N(R23)- d-6 alkyl; N(R23)-C(0)- d-e alkyl; S(0)2N(R23)-C1-6 alkyl; S(0)N(R23)-C1-6 alkyl; S(0)2-C1-6 alkyl; S(0)-C1-6 alkyl; N(R23)S(0)2-C1-6 alkyl; and N(R23)S(0)-C .6 alkyl, wherein each d-e alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R19 is selected from the group consisting of phenyl; and naphthyl, wherein R19 is optionally substituted with one or more R24, wherein R24 is independently selected from the group consisting of halogen; CN; COOH; OH; C(0)NH2; S(0)2NH2; S(0)NH2; d-6 alkyl; 0-C1-6 alkyl; S-d-e alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R25)-d-6 alkyl; S(0)2N(R25)-d.6 alkyl; S(0)N(R25)-d-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R25)S(0)2-d-6 alkyl; and N(R25)S(0) -d-6 alkyl, wherein each Ci-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R20 is selected from the group consisting of heterocycle; heterobicycle; and C3-7 cycloalkyl; wherein R20 is optionally substituted with one or more R26, wherein R26 is independently selected from the group consisting of halogen; CN; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(O)NH2; S(0)2NH2; S(0)NH2; Ci-6 alkyl; 0-d-6 alkyl; S-d.6 alkyl; N(R27)-C1-6 alkyl; COO-d-6 alkyl; OC(0)-Cι-6 alkyl; C(O)N(R27)- d-6 alkyl; N(R27)-C(0)-d-6 alkyl; S(0)2N(R27)-Cι-6 alkyl; S(0)N(R27)-d-6 alkyl; S(0)2-C1.6 alkyl; S(0)-C1-6 alkyl; N(R27)S(0)2-d-6 alkyl; and N(R )S(0)-d-6 alkyl wherein each Cι-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R21, R23, R25, R27 are independently selected from the group consisting of H; and d-6alkyl, which is optionally substituted with one or more of R28, wherein R28 is independently selected frorri the group consisting of F; Cl and OH;
R6, R7 are independently selected from the group consisting of H; (C(R29R30))m-X1-Z1; and (C(R31R32))n-X2-X3-Z2, provided that R6, R7 are selected so that not both of R6, R7 are independently selected from the group consisting of H; CH3; CH2CH3; CH2CH2CH3; and CH(CH3)2;
Optionally R6, R7 are independently C-ι- alkyl, which is substituted with one or more R29a, wherein R29a is independently selected from the group consisting of R29b; and Z1, provided that R6, R7 are selected so that not both of R6, R7 are independently selected from the group consisting of CH3; CH2CH3; CH2CH2CH3; and CH(CH3)2;
R29, R29 , R30, R31, R32 are independently selected from the group consisting of H; halogen; CN; OH; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; 0-d-6 alkyl; N(R32a)-d-6 alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R32a)- d-6 alkyl; N(R32a)-C(O)-d-6 alkyl; S(0)2N(R32a)-d-e alkyl; S(O)N(R32a)-d.6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R32a)S(0)2-d-6 alkyl; and N(R32a)S(0)-Cι-6 alkyl wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl; R32a is selected from the group consisting of H; and Cι-6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Optionally one or more pairs of R29, R30, R31, R32 independently selected from the group consisting of R29/R30; and R31/R32 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more R32 , wherein R32b is independently selected from the group consisting of F; Cl; and OH;
m is O, 1 , 2, 3 or 4;
n is 2, 3 or 4;
X1 is independently selected from the group consisting of a covalent bond -d-6„ alkyl-; -d-6 alkyl-O-; -d-e alkyl-N(R33)-; -C(O)-; -C(0)-d-e alkyl- -C(0) d-6 alkyl-O-; -C(0)-d-6 alkyl-N(R33)-; -C(0)0-; -C(0)0-d-6 alkyl- -C(0)0-d-6 alkyl-O-; -C(0)0-d-6 alkyl-N(R33)-; -C(0)N(R33)-; -C(0)N(R33)-d-6 alkyl- -C(0)N(R33)-d-6 alkyl-O-; -C(0)N(R33)-d-6 alkyl-N(R34)-; -S(0)2-; -S(O)- -S(0)2-Ci_6 alkyl-; -S(0)-d-6 alkyl-; -S(0)2-d-e alkyl-O-; -S(0)-d-6 alkyl-O- -S(0)2-d.6 alkyl-N(R33)-; and -S(O)-d-6 alkyl-N(R33)-; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
X2 is selected from the group consisting of -0-; -S-; -S(O)-; S(0)2-; and -N(R35)-;
X3 is selected from the group consisting of a covalent bond; -Cι.6 alkyl-; -d-6 alkyl-O- -d-β alkyl-N(R36)-; -C(O)-; -C(0)-d-6 alkyl-; -C(0)-d-6 alkyl-O-; -C(0)-d-6 alkyl-N(R36)- -C(0)0-; -C(0)0-d-e alkyl-; -C(O)O-d-6 alkyl-O-; -C(0)0-d-e alkyl-N(R36)- -C(0)N(R36)-; -C(0)N(R36)-C1-6 alkyl-; -C(0)N(R36)-d-6 alkyl-O-; and -C(0)N(R36)-d-6 alkyl-N(R37)-; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Optionally X2-X3 are independently selected from the group consisting of -N(R35)-S(0)2; -N(R35)-S(0)-; -N(R35)-S(0)2-d-6 alkyl-; -N(R35)-S(0)-d-6 alkyl-; -N(R35)-S(0)2-d-6 alkyl-O-; -N(R35)-S(0)-C1-6 alkyl-O-; -N(R35)-S(0)2-d-6 alkyl-N(R36)-; and -N(R35)-S(0)-d-6 alkyl-N(R36)-; wherein each C-ι-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R33, R34, R35, R36, R37 are independently selected from the group consisting of H; and Ci-6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Z1, Z2 are independently selected from the group consisting of Z3; and -C(R37a)Z3aZ3b;
R37a is selected from the group consisting of H; and d-6 alkyl, which is optionally substituted with one or more F;
Z3, Z3a, Z3b are independently selected from the group consisting of H; T1; T2; Ci-6 alkyl; d-6 alkyl-T1; and Cι-6 alkyl-T2; wherein each Ci-6 alkyl is optionally substituted with one or more R37b, wherein R37b is independently selected from the group consisting of halogen; CN; OH; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; Cι-6 alkyl O-d-e alkyl; N(R37c)-d-6 alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R37c)- d-6 alkyl N(R370)-C(O)-C1-6 alkyl; S(0)2N(R37c)-d-6 alkyl; S(0)N(R37c)-d-6 alkyl; S(0)2-d-6 alkyl S(0)-d-6 alkyl; N(R37°)S(0)2-C1-6 alkyl; and N(R37c)S(0)-d-6 alkyl; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
T1 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T1 is optionally substituted with one or more R38; wherein R38 is independently selected from the group consisting of halogen; CN; R39; COOH; OH; C(O)NH2; S(0)2NH2; S(0)NH2; COOT3; OT3; ST3; C(O)N(R40)T3; S(O)2N(R40)T3; S(O)N(R40)T3 and T3;
T2 is selected from the group consisting of C3-7 cycloalkyl; indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle; wherein T2 is optionally substituted with one or more R41, wherein R41 is independently selected from the group consisting of halogen; CN; R42; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(0)NH2; S(0)2NH2; S(O)NH2; COOT3; OT3; C(0)N(R43)T3; S(0)2N(R43)T3; S(0)N(R43)T3; N(R43)T3; and T3; R39 is selected from the group consisting of C1-6 alkyl; O-C1-6 alkyl; S-d-6 alkyl; COO-d-6 alkyl; OC(O)- d-6 alkyl; C(0)N(R44)-C1-6 alkyl; S(0)2N(R44)-d-6 alkyl; S(0)N(R44)-d-e alkyl; S(0)-d-6 alkyl; S(0)2-d-6 alkyl; N(R44)S(0)2-d-6 alkyl; and N(R44)S(0) -Ci-6 alkyl; wherein each .6 alkyl is optionally substituted with one more R45, wherein R45 is independently selected from the group consisting of F; COOR46; C(0)N(R46R47); S(0)2N(R46R47); OR46; N(R46R47); T3; O-T3; and N(R46)-T3;
R42 is selected from the group consisting of C1-6 alkyl; 0-d-6 alkyl; S-C1-6 alkyl; N(R48)-d-6 alkyl; COO-d-6 alkyl; OC(O)- d-6 alkyl; C(0)N(R48)- d-6 alkyl; N(R48)-C(0)- d-e alkyl; S(0)2N(R48)-C1-6 alkyl; S(O) N(R48)-d-6 alkyl; S(0)-d-6 alkyl; S(0)2-d-6 alkyl; -N(R48)S(0)2-d-6 alkyl; and -N(R48)S(0)-C1.6 alkyl; wherein each d-6 alkyl is optionally substituted with one or more R45, wherein R45 is independently selected from the group consisting of F; COOR49; C(0)N(R49R50); S(0)2N(R49R5°); S(0)N(R49R50); OR49; N(R49R50); T3; O-T3; and N(R49)-T3;
R40, R43, R44, R46, R47, ' R4?, R49, R50 are independently selected from the group consisting of H; and d-6 alkyl;
T3 is selected from the group consisting of T4; and T5;
T4 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T4 is optionally substituted with one or more R51, wherein R51 is independently selected from the group consisting of halogen; CN; COOR52; OR52; C(0)N(R52R53); S(0)2N(R52R53); Ci-6 alkyl; O-C1-6 alkyl; S-Cι-6 alkyl; COO-d-6 alkyl; OC(0)-Cι.6 alkyl; C(0)N(R52)- Ci-6 alkyl; S(0)2N(R52)-d-6 alkyl; S(0)N(R52)-d-6 alkyl; S(0)2-d-6 alkyl; S(O) -C1-6 alkyl; N(R52)S(0)2-d-6 alkyl; and N(R52)S(0)-d-6 alkyl; wherein each -e alkyl is optionally substituted with one more halogen selected from the group consisting of F; and Cl;
T5 is selected from the group consisting of heterocycle; heterobicycle; C3.7 cycloalkyl; indanyl; tetralinyl; and decalinyl; wherein T5 is optionally substituted with one or more R54, wherein R54 is independently selected from the group consisting of halogen; CN; OR55; oxo (=0), where the ring is at least partially saturated; N(R55R56); COOR55; C(0)N(R55R56); S(0)2N(R55R56); S(0)N(R55R56); Cι-6 alkyl; 0-d-6 alkyl; S-d-6 alkyl; N(R55)-d-6 alkyl; COO-d-6 alkyl; OC(O)- Cw alkyl; C(0)N(R55)- Cι-6 alkyl; N(R55)-C(0)-d-6 alkyl; S(0)2N(R55)-d-6 alkyl; S(0)N(R55)-Cι-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R55)S(0)2-C1.6 alkyl; and N(R55)S(0)-d-6 alkyl; wherein each C1-6 alkyl is optionally substituted with one more halogen selected from the group consisting of F; and Cl;
R52, R53, R55, R56, are independently selected from the group consisting of H; and C1-6 alkyl.
Within the meaning of the present invention the terms are used as follows:
In case a variable or substituent can be selected from a group of different variants and such variable or substituent occurs more than once the respective variants can be the same or different.
"Alkyl" . means a straight-chain or branched carbon chain that may contain double or triple bonds. It is generally preferred that alkyl doesn't contain double or triple bonds. "Cι-4 Alkyl" means an alkyl chain having 1 - 4 carbon atoms, e.g. at the end of a molecule methyl, ethyl, -CH=CH2, -C≡CH, n-propyl, isopropyl, -CH=CH-CH3, -CH2- CH=CH2, n-butyl, isobutyl, -CH=CH-CH2-CH3, -CH=CH-CH=CH2, sec-butyl tert-butyl or amid, e.g. -CH2-, -CH2-CH2-, -CH=CH-, -CH(CH3)-, -C(CH2)-, -CH2-CH2-CH2-, - CH(C2H5)-, -CH(CH3)2-.
"d-6 Alkyl" means an alkyl chain having 1 - 6 carbon atoms, e.g. d- alkyl, methyl, ethyl, -CH=CH2, -C≡CH, n-propyl, isopropyl, -CH=CH-CH3, -CH2-CH=CH2, n-butyl, isobutyl, -CH=CH-CH2-CH3, -CH=CH-CH=CH2, sec-butyl tert-butyl, n-pentane, n-hexane, or amid, e.g. -CH2-, -CH2-CH2-, -CH=CH-, -CH(CH3)-, -C(CH2)-, -CH2-CH2-CH2-, -CH(C2H5)-, -CH(CH3)2-. Each hydrogen of a Cι-6 alkyl carbon may be replaced by a substituent.
"C3-7 Cycloalkyl" or "C3-7 Cycloalkyl ring" means a cyclic alkyl chain having 3 - 7 carbon atoms, e.g. cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl. Each hydrogen of a cycloalkyl carbon may be replaced by a substituent. "Halogen" means fluoro, chloro, bromo or iodo. It is generally preferred that halogen is fluoro or chloro.
"Heterocycle" means a cyclopentane, cyclohexane or cycloheptane ring that may contain up to the maximum number of double bonds (aromatic or non-aromatic ring which is fully, partially or un-saturated) wherein at least one carbon atom up to 4 carbon atoms are replaced by a heteroatom selected from the group consisting of sulfur (including -S(O)-, -S(0)2-), oxygen and nitrogen (including =N(0)-) and wherein the ring is linked to the rest of the molecule via a carbon or nitrogen atom. Examples for a heterocycle are furan, thiophene, pyrrole, pyrroline, imidazole, imidazoline, pyrazole, pyrazoline, oxazole, oxazoline, isoxazole, isoxazoline, thiazole, thiazoline, isothiazole, isothiazoline, thiadiazole, thiadiazoline, tetrahydrofuran, tetrahydrothiophene, pyrrolidine, imidazolidine, pyrazolidine, oxazolidine, isoxazolidine, thiazolidine, isothiazolidine, thiadiazolidine, sulfolane, pyran, dihydropyran, tetrahydropyran, imidazolidine, pyridine, pyridazine, pyrazine, pyrimidine, piperazine, piperidine, morpholine, tetrazole, triazole, triazolidine, tetrazolidine, azepine or homopiperazine. :-'
"Heterobicycle" means a heterocycle which is condensed with phenyl or an additional heterocycle to form a bicyclic ring system. "Condensed" to form a bicyclic ring means that two rings are attached to each other by sharing two ring atoms. Examples for a heterobicycle are indole, indoline, benzofuran, benzothiophene, benzoxazole, benzisoxazole, benzothiazole, benzisothiazole, benzimidazole, benzimidazoline, quinoline, quinazoline, dihydroquinazoline, dihydroquinoline, isoquinoline, tetrahydroisoquinoline, dihydroisoquinoiine, benzazepine, purine or pteridine.
A preferred stereochemistry of compounds or a pharmaceutically acceptable salt thereof according to the present invention is shown in formula (la)
Figure imgf000012_0001
wherein Z, R^R' and X have the meaning as indicated above. Preferred compounds of formula (I) or (la) are those compounds in which one or more of the residues contained therein have the meanings given below, with all combinations of preferred substituent definitions being a subject of the present invention. With respect to all preferred compounds of the formulas (I) or (la) the present invention also includes all tautomeric and stereoisomeric forms and mixtures thereof in all ratios, and their pharmaceutically acceptable salts.
In preferred embodiments of the present invention, the substituents Z, R1-R7 and X of the formula (I) or (la) independently from each other have the following meaning. Hence, one or more of the substituents Z, R1-R7 and X can have the preferred or more preferred meanings given below.
Z is as defined above. Preferably, Z is phenyl or heterocycle. When Z is a heterocycle, it is preferably an aromatic heterocycle.
Preferably, Z is optionally substituted with 1 , 2 or 3, in one embodiment 1 or 2, R8, which are the same or different. Preferably, they are the same.
R8 is as defined above. Preferably, R8 is selected from the group consisting of Cl; F; CN; CH3; and OCH3, more preferably Cl, F or CN, most preferably F.
In a more preferred embodiment Z is 2-Fluoro-phenyl. In another preferred embodiment Z is 2,4,5-Trifluoro-phenyl.
R1, R4 are independently as defined above. Preferably, R1, R4 are independently selected from the group consisting of H; F; OH; CH3; and OCH3, more preferably H or F.
R2, R5 are independently as defined above. Preferably, R2, R5 are independently selected from the group consisting of H; F; and CH3, more preferably H or F.
R1, R2, R4, R5 are more preferred H.
R3 is as defined above. Preferably, R3 is H. X is as defined above. Preferably, X is C(O). In another embodiment, X is preferably S(O)2.
R6 is as defined above. R6 is preferably selected from the group consisting of H, d- alkyl and C3-7 cycloalkyl, more preferably H, CH3 and cyclopropyl, most preferably H and CH3.
In one embodiment, R6 is preferably CH(R29a)2; CHR29a-CH2R29a; CH2-CH(R29a)2; CH2- CHR29a-CH2R29a; and CH2-CH2-CH(R29a)2. In this embodiment, both R 9a are preferably different, wherein one R29a is R29b and the other R29a is Z1; wherein Z1 is preferably selected from T2. Preferred embodiments for R29b and T2 are as set forth below. Most preferably, T2 is 5- or 6-membered heterocycle, preferably a saturated aromatic heterocycle containing preferably at least one N and optionally an O. Most preferably Z2 is pyridyl or morpholinyl.
XI is as.defined above: Preferably, X1 is a covalent bond.
m is as defined above. Preferably, m is 0, 1 , 2 or 3, more preferably 0 or 1.
R7 is as defined above. Preferably, R7 is Z1. In this case, Z1 is preferably T2, wherein T2 is as defined above, preferably selected from the group consisting of C3.7 cycloalkyl; indanyl; tetralinyl; decalinyl; and heterobicycle, more preferably indanyl; tetralinyl; and decalinyl; wherein T2 is optionally substituted with one or more R4 . It is preferred that T2 is unsubstituted or substituted with one R41. R4 is as defined above, preferably halogen, methyl, O-methyl or OH, most preferred is OH.
Preferably, R7 is Cι- alkyl, substituted with 1-4, i.e. 1 , 2, 3 or 4, preferably 1 or 2, R29a, which are the same or different.
R7 is more preferred selected from the group consisting of CH(R29a)2; CHR29a-CH2R29a; CH2-CH(R29a)2; CH2-CHR29a-CH2R29a; and CH2-CH2-CH(R29a)2. In one embodiment, R7 is selected from the group consisting of CHR29a-CH(CH3)2. In one embodiment, R7 is (C(R31R3 ))n-X2-X3-Z2. In this embodiment, R31 and R32 are preferably both H. Integer n is preferably 1 or 2, more preferably 1. X2 is preferably NH or O, more preferably O. X3 is preferably a covalent bond, CH2j or C(O), more preferably C(O). Z2 is preferably T2, more preferably a 5- or 6-membered heterocycle, preferably an aromatic heterocycle containing preferably at least one N. Most preferably Z2 is pyridyl.
R29a is as defined above. Preferably, R29a is selected from the group consisting of R29b; and Z1; wherein R29b is preferably selected from the group consisting of H, halogen, N(R32a)-d-6 alkyl, NH2, and d-6 alkyl, more preferably H; F; Cl; NH2; NHCH3; N(CH3)2; CH3; and C2H5. Most preferably, R29b is selected from the group consisting of H; N(CH3)2 and CH3.
Preferably, R29a is selected from the group consisting of R29b; and Z1; wherein Z1 is selected from the group consisting of T1; and T2.
In one embodiment, two R29a are present and both R29a are preferably different, wherein one R29a is R29b and the other R29a is Z1; wherein Z is preferably selected from the group consisting of T and T2.
In one embodiment, two R29a are present and both R29a are preferably the same or different, more preferably different, and are Z1; wherein Z1 is selected from the group consisting of T1 and T2. In this embodiment, it is preferred that R7 is selected from the group consisting of CH(R 9a)2; CHR29a-CH2R29a; CH2-CHR29a-CH2R29a; and CH2-CH2- CH(R29a)2, more preferably CH2-CH(R29a)2 and CH2-CHR29a-CH2R29a. It is particularly preferred that both of R29a are selected from T1; or that one R29a is selected from T1 and the other one R29a is selected from T2. Specifically, in the case of CH2-CHR29a-CH2R29a, CH2-CHT1-CH2T2 is preferred.
T1 is as defined above. Preferably, T1 is phenyl; wherein T1 is optionally substituted with 1-3, i.e. 1 , 2 or 3, more preferably 1 or 2, most preferably 1 , R38, which are the same or different.
R38 is as defined above. Preferably, R38 is independently selected from the group consisting of halogen, CN, S02NH2, CONH2, Cι-6 alkyl optionally substituted with 1 , 2 or 3 R45; 0-d-6 alkyl optionally substituted with 1 R45; S-d-6 alkyl; S(0)-d_6 alkyl; S(O)- d-6 alkyl; S(0)2-d-6 alkyl; CONHT3; CONH-d-6 alkyl optionally substituted with 1 R45; T3; and O-T3.
More preferably, R38 is independently selected from the group consisting of halogen; CN; S02NH2; CONH2;
Cι-4 alkyl optionally substituted with 1 , 2 or 3 F;
0-d-6 alkyl optionally substituted with 1 or 2 F or N(R49R50);
S-d-6 alkyl; S(0)-d-6 alkyl; S(0)2-d-6 alkyl; CONH-C3.7 cycloalkyl; CONH-heterocyclyl;
CONH-Cι-4 alkyl optionally substituted with 1 phenyl optionally substituted with 1 or 2
R51; phenyl optionally substituted with 1 or 2 R51; heterocyclyl;
O-heterocyclyl; and
O-phenyl optionally substituted with 1 or 2 R5 .
It is most preferred that R38 is independently selected from the group consisting of F;
Cl; CN; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; 0-CH3; 0-C2H5; S-CH3; S(0)-CH3;
S(0)2-CH3; S02NH2; CONH2;
O-ethyl substituted with 1 N(Me)2;
O-methyl substituted with 2 F;
CONH-cyclopropyl; CONH-pyridyl;
CONH-d-4 alkyl substituted with 1 phenyl optionally substituted with 1 or 2 F or
S02NH2; phenyl;
O-pyrimidyl; O-pyrazinyl;
Figure imgf000016_0001
and
O-phenyl optionally substituted with 1 R51.
In one embodiment, R38 is independently selected from the group consisting of F; Cl; CN; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; 0-CH3; 0-C2H5; S-CH3; S02NH2; T3; and O-T3.
In particular in the case of the presence of two R29a whereby one R29a is selected from T1 and the other one R29a is selected from T2, T1 is preferably phenyl; wherein T1 is unsubstituted or substituted with 1-3, i.e. 1 , 2 or 3, more preferably 1 or 2, most preferably 1 , R38 selected from F; Cl; CN; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; O- CH3; 0-C2H5; S-CH3; S02NH2; T3; and O-T3, more preferably F; Cl; CN; CH3, most preferably F or Cl.
T2 is as defined above. Preferably, T2 is selected from the group consisting of indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle; more preferably
- tetralinyl;
- a 4-, 5- or 6-membered, preferably a 5- or 6-membered, heterocycle containing at least one heteroatom, preferably N or O, whereby the heterocycle can be selected from the group of (a) aromatic, preferably 5- or 6-membered ring and preferably containing at least one heteroatom selected from N, more preferably containing 1 or 2 N and optionally an O, most preferably selected from the group
Figure imgf000017_0001
(b) saturated, preferably 4-, 5- or 6-membered ring and preferably containing at i least one heteroatom selected from N, more preferably containing 1 or 2 N and optionally an O, most preferably selected from the group
Figure imgf000018_0001
or
- a 9- or 10-membered heterobicycle containing at least one heteroatom, preferably N or O, and optionally a further heteroatom selected from N and O, most preferably selected from the group
Figure imgf000018_0002
In one embodiment, T is selected from the group consisting of
Figure imgf000019_0001
Figure imgf000019_0002
In particular in the case of the presence of two R whereby one R is selected from T1 and the other one R29a is selected from T2 or both R29a are T2, T2 is preferably a N- containing heterocycle, preferably containing 4, 5 or 6 ring members and optionally containing a further heteroatom selected from N or O. Most preferably T2 is selected from the group
Figure imgf000019_0003
T2 is optionally substituted with 1 , 2 or 3, preferably 1-2 R41, which are the same or different. Preferably R41 is independently selected from the group consisting of halogen; CN; OH; Cι-6 alkyl optionally substituted with 1 , 2 or 3 F; O-C-i-e alkyl substituted with 1 , 2 or 3 F; NH-C(0)-d-6 alkyl; and T3. More preferably R4 is selected from the group consisting of
F; Cl; OH;
Cι-4 alkyl optionally substituted with 1, 2 or 3 F;
NH-C(0)-d-4 alkyl;
T4 optionally substituted with 1 or 2, more preferably 1 , R51, whereby R51 is as defined herein; or
C3-7 cycloalkyl.
Most preferably R41 is selected from the group consisting of
F; CI;
Cι-2 alkyl optionally substituted with 3 F;
NH-C(0)-d-2 alkyl; phenyl optionally substituted with F; Cl; CH3; C2H5; 0-CH3; or 0-C2H5lor cyclopropyl.
R41 is as defined above. Preferably, R41 is selected from the group consisting of OH; CH3; and T3;
T3 is as defined above. Preferably, T3 is T4.
T4 is as defined above. Preferably, T4 is phenyl, wherein T4 is optionally substituted with 1-3 R5 , which are the same or different.
R51 is as defined above. Preferably, R51 is independently selected from the group consisting of F; Cl; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; 0-CH3; 0-C2H5; S-CH3; and S02NH2.
Preferably, T 3 i is T T5 T5 is as defined above. Preferably, T5 is heterocycle, more preferably a 5- or 6- membered heterocycle. The heterocycle may be saturated or aromatic. The heterocycle may contain at least one N. Preferred examples of the heterocycle include:
Figure imgf000021_0001
T is optionally substituted with 1-2 R , which are the same or different.
R54 is as defined above. Preferably, R54 is selected from the group consisting of OH; and CH3.
Particularly preferred definitions for -NR6R7are as follows:
21
Figure imgf000022_0001
Figure imgf000023_0001
Figure imgf000024_0001
Figure imgf000025_0001
25
Figure imgf000026_0001
Compounds of the formula (I) or (la) in which some or all of the above-mentioned groups have the preferred or more preferred meanings are also an object of the present invention.
Furthermore, the present invention provides prodrug compounds of the compounds of the invention as described above.
"Prodrug compound" means a derivative that is converted into a compound according to the present invention by a reaction with an enzyme, gastric acid or the like under a physiological condition in the living body, e.g. by oxidation, reduction, hydrolysis or the like, each of which is carried out enzymatically. Examples of the prodrug are compounds, wherein the amino group in a compound of the present invention is acylated, alkylated or phosphorylated to form, e.g., eicosanoylamino, alanylamino, pivaloyloxymethylamino or wherein the hydroxyl group is acylated, alkylated, phosphorylated or converted into the borate, e.g. acetyloxy, palmitoyloxy, pivaloyloxy, succinyloxy, fumaryloxy, alanyloxy or wherein the carboxyl group is esterified or amidated. These compounds can be produced from compounds of the present invention according to well-known methods.
Metabolites of compounds of formula (1) or (la) are also within the scope of the present invention.
Where tautomerism, like e.g. keto-enol tautomerism, of compounds of general formula (I) or (la) or their prodrugs may occur, the individual forms, like e.g. the keto and enol form, are claimed separately and together as mixtures in any ratio. Same applies for stereoisomers, like e.g. enantiomers, cis/trans isomers, conformers and the like. If desired, isomers can be separated by methods well known in the art, e.g. by liquid chromatography. Same applies for enantiomers by using e.g. chiral stationary phases. Additionally, enantiomers may be isolated by converting them into diastereomers, i.e. coupling with an enantiomerically pure auxiliary compound, subsequent separation of the resulting diastereomers and cleavage of the auxiliary residue. Alternatively, any enantiomer of a compound of formula (I) or (la) may be obtained from stereoselective synthesis using optically pure starting materials. In case the compounds according to formula (I) or (la) contain one or more acidic or basic groups, the invention also comprises their corresponding pharmaceutically or toxicologically acceptable salts, in particular their pharmaceutically utilizable salts. Thus, the compounds of the formula (I) or (la) which contain acidic groups can be present on these groups and can be used according to the invention, for example, as alkali metal salts, alkaline earth metal salts or as ammonium salts. More precise examples of such salts include sodium salts, potassium salts, calcium salts, magnesium salts or salts with ammonia or organic amines such as, for example, ethylamine, ethanolamine, triethanolamine or amino acids. Compounds of the formula (I) or (la) which contain one or more basic groups, i.e. groups which can be protonated, can be present and can be used according to the invention in the form of their addition salts with inorganic or organic acids. Examples for suitable acids include hydrogen chloride, hydrogen bromide, phosphoric acid, sulfuric acid, nitric acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisulfonic acids, oxalic acid, acetic acid, tartaric acid, lactic acid, salicylic acid, benzoic acid, formic acid, propionic acid, pivalic acid, diethylacetic acid, malonic acid, succinic acid, pimelic acid, fumaric acid, maleic acid, malic acid, sulfaminic acid, phenylpropionic acid, gluconic acid, ascorbic acid, isonicotinic acid, citric acid, adipic acid, and other acids known to the person skilled in the art. If the compounds of the formula (I) or (la) simultaneously contain acidic and basic groups in the molecule, the invention also includes, in addition to the salt forms mentioned, inner salts or betaines (zwitterions). The respective salts according to the formula (1) or (la) can be obtained by customary methods which are known to the person skilled in the art like, for example by contacting these with an organic or inorganic acid or base in a solvent or dispersant, or by anion exchange or cation exchange with other salts. The present invention also includes all salts of the compounds of the formula (I) or (la) which, owing to low physiological compatibility, are not directly suitable for use in pharmaceuticals but which can be used, for example, as intermediates for chemical reactions or for the preparation of pharmaceutically acceptable salts.
The present invention provides compounds of general formula (I) or (la) or their prodrugs as DPP-IV inhibitors. DPP-IV is a cell surface protein that has been implicated in a wide range of biological functions. It has a broad tissue distribution (intestine, kidney, liver, pancreas, placenta, thymus, spleen, epithelial cells, vascular endothelium, lymphoid and myeloid cells, serum), and distinct tissue and cell-type expression levels. DPP-IV is identical to the T cell activation marker CD26, and it can cleave a number of immunoregulatory, endocrine, and neurological peptides in vitro. This has suggested a potential role for this peptidase in a variety of disease processes.
DPP-IV related diseases are described in more detail in WO-A-03/181 under the paragraph "Utilities" which is herewith incorporated by reference.
Accordingly, the present invention provides compounds of formula (I) or (la) or their prodrugs or pharmaceutically acceptable salt thereof for use as a medicament.
Furthermore, the present invention provides the use of compounds of formula (I) or (la) or their prodrugs or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for the treatment or prophylaxis of non-insulin dependent (Type II) diabetes mellitus; hyperglycemia; obesity; insulin resistance; lipid disorders; dyslipidemia; hyperlipidemia; hypertriglyceridemia; hypercholestrerolemia; low HDL; high LDL; atherosclerosis; growth hormone deficiency; diseases related to the immune response; HIV infection; neutropenia; neuronal disorders; tumor metastasis; benign prostatic hypertrophy; gingivitis; hypertension; osteoporosis; diseases related to sperm motility; low glucose tolerance; insulin resistance; ist sequelae; vascular restenosis; irritable bowel syndrome; inflammatory bowel disease; including Crohn's disease and ulcerative colitis; other inflammatory conditions; pancreatitis; abdominal obesity; neurodegenerative disease; anxiety; depression; retinopathy; nephropathy; neuropathy; Syndrome X; ovarian hyperandrogenism (polycystic ovarian syndrome; Type n diabetes; or growth hormone deficiency. Preferred is non-insulin dependent (Type II) diabetes mellitus and obesity.
The present invention provides pharmaceutical compositions comprising a compound of formula (I) or (la), or a prodrug compound thereof, or a pharmaceutically acceptable salt thereof as active ingredient together with a pharmaceutically acceptable carrier.
"Pharmaceutical composition" means one or more active ingredients, and one or more inert ingredients that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutically acceptable carrier.
A pharmaceutical composition of the present invention may additionally comprise one or more other compounds as active ingredients like one or more additional compounds of formula (I) or (la), or a prodrug compound or other DPP-IV inhibitors. Other active ingredients are disclosed in WO-A-03/181 under the paragraph "Combination Therapy" which is herewith incorporated by reference. Accordingly, other active ingredients may be insulin sensitizers; PPAR agonists; biguanides; protein tyrosinephosphatase-lB (PTP-1 B) inhibitors; insulin and insulin mimetics; sulfonylureas and other insulin secretagogues; a-glucosidase inhibitors; glucagon receptor antagonists; GLP-1 , GLP-1 mimetics, and GLP-1 receptor agonists; GIP, GIP mimetics, and GIP receptor agonists; PACAP, PACAP mimetics, and PACAP receptor 3 agonists; cholesterol lowering agents; HMG-CoA reductase inhibitors; sequestrants; nicotinyl alcohol; nicotinic acid or a salt thereof; PPARa agonists; PPARoly dual agonists; inhibitors of cholesterol absorption; aeyl CoA : cholesterol acyltransferase inhibitors; anti-oxidants; PPARo agonists; antiobesity compounds; an ileal bile acid transporter inhibitor; or anti-inflammatory agents or pharmaceutically acceptable salts of these active compounds.
The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids, including inorganic bases or acids and organic bases or acids.
The compositions include compositions suitable for oral, rectal, topical, parenteral (including subcutaneous, intramuscular, and intravenous), ocular (ophthalmic), pulmonary (nasal or buccal inhalation), or nasal administration, although the most suitable route in any given case will depend on the nature and severity of the conditions being treated and on the nature of the active ingredient. They may be conveniently presented in unit dosage form and prepared by any of the methods well- known in the art of pharmacy.
In practical use, the compounds of formula (I) or (la) can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). In preparing the compositions for oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like in the case of oral liquid preparations, such as, for example, suspensions, elixirs and solutions; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like in the case of oral solid preparations such as, for example, powders, hard and soft capsules and tablets, with the solid oral preparations being preferred over the liquid preparations.
Because of their ease of administration, tablets and capsules represent the most advantageous oral dosage unit form in which case solid pharmaceutical carriers are obviously employed. If desired, tablets may be coated by standard aqueous or nonaqueous techniques. Such compositions and preparations should contain at least 0.1 percent of active compound. The percentage of active compound in these compositions may, of course, be varied' arid may conveniently be between about 2 percent to about 60 percent of the weight of the unit. The amount of active compound in such therapeutically useful compositions is such that an effective dosage will be obtained. The active compounds can also be administered intranasally as, for example, liquid drops or spray.
The tablets, pills, capsules, and the like may also contain a binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin. When a dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil.
Various other materials may be present as coatings or to modify the physical form of the dosage unit. For instance, tablets may be coated with shellac, sugar or both. A syrup or elixir may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor. Compounds of formula (I) or (la) may also be administered parenterally. Solutions or suspensions of these active compounds can be prepared in water suitably mixed with a surfactant such as hydroxy-propylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils. Any suitable route of administration may be employed for providing a mammal, ■ especially a human, with an effective dose of a compound of the present invention. For example, oral, rectal, topical, parenteral, ocular, pulmonary, nasal, and the like may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols, and the like. Preferably compounds of formula (I) or (la) are administered orally.
The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration, the condition being treated and the severity of the condition being treated. Such dosage may be ascertained readily by a person skilled in the art.
When treating or preventing diabetes mellitus and/or hyperglycemia or hypertriglyceridemia or other diseases for which compounds of Formula I are indicated, generally satisfactory results are obtained when the compounds of the present invention are administered at a daily dosage of from about 0.1 milligram to about 100 milligram per kilogram of animal body weight, preferably given as a single daily dose or in divided doses two to six times a day, or in sustained release form. For most large mammals, the total daily dosage is from about 1.0 milligrams to about 1000 milligrams, preferably from about 1 milligrams to about 50 milligrams. In the case of a 70 kg adult human, the total daily dose will generally be from about 7 milligrams to about 350 milligrams. This dosage regimen may be adjusted to provide the optimal therapeutic response.
Preferred embodiments of compounds having formula (I) of the present invention can be prepared from beta amino acid intermediates such as those of formula (II)
Figure imgf000033_0001
(II)
and substituted amine intermediates such as those of formula (III)
HN(R6R7) (III),
using standard peptide coupling conditions.
Some abbreviations that may appear in this application are as follows.
ABBREVIATIONS
Designation Boc (or BOC) ferf-Butoxycarbonyl
DCM Dichloromethane
DIEA Diisopropylethylamine
DMF Λ/,/V-Dimethylformamide
DMSO Dimethylsulfoxide
EDC 1 -Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
Et3N Triethylamine h Hour
HATU O-(7-Azabenzotriazol-1-yl)-Λ/,Λ/,Λ/' Λ/'-tetramethyluronium hexafluorophosphate HBTU 0-(7-benzotriazol-1-yl)-Λ/,Λ/,Λ/',Λ/'-tetramethyluronium hexafluorophosphate
HOBt 1 -Hydroxybenzotriazole
HPLC High pressure liquid chromatography min Minutes
MTP Microtiter plate
PG Protecting group
Pol Polymeric support rt Retention time
TFA Trifluoroacetic acid
THF Tetrahydrofuran
Available starting materials may be amines having the formula (III), which may be purchased from commercially available sources such as ABCR, Array, Astatech, Sigma-Aldrich, Fluka, or be synthesized by one skilled in the art. Common nucleophilic substitution reactions between compounds containing a suitable leaving group (e.g. halogenide, mesylate, tosylate, epoxides) and nucleophiles (e.g. amines) may be employed. The conversion of diverse functional groups may allow the synthesis of various amines, e.g. conversion of esters into acids, alcohols or amides intermediates; reduction of amides, nitriles or azides to amines; also novel carbon-nitrogen palladium- catalyzed coupling reactions with suitable functionalized starting materials. For the introduction of changes in the carbon chain attached to the nitrogen atom or for the synthesis of diverse (hetero)aryl derivatives, it may be possible to make use of diverse carbon-carbon coupling reactions, e.g. transition-metal catalyzed reactions, conventional techniques for ring closure, formylation of (hetero)aryls.
Schemes A through G outline general procedures for the synthesis of some compounds (III) described below. Unless otherwise indicated in the schemes, the variables have the same meaning as described above. The variable cy is selected from a group of cyclic ring systems such as aryles, heterocylces and heterobicycles. Scheme A
H protection CH, / 3 H-N H-N Re reduction R°
Scheme B
Figure imgf000035_0001
Scheme C
Figure imgf000035_0002
Scheme D
Figure imgf000035_0003
deprotection Step 3
Figure imgf000035_0004
Scheme E
Figure imgf000036_0001
Scheme F
Figure imgf000036_0002
Scheme G
Figure imgf000036_0003
condensation Step 3
Figure imgf000036_0004
Enantiomerically pure beta amino acids having the formula (lla)
Figure imgf000036_0005
may be commercially available, known in the literature or may be conveniently synthesized using one of the methods already published and reviewed in e.g., Cole, Tetrahedron, 32, 9517 (1994), Juaristi et al., Aldrichimica Acta, 27, 3, 1994, or Juaristi, Enantioselective Synthesis of β-Amino Acids, Ed. Wiley-VCH, New York, 1997.
In particular, 3-amino-4-(2,4,5-trifluoro-phenyl)-butyric acid may be synthesized by a variety of methods as reported in the patent applications WO 2004069162, WO 2004064778, WO 2004037169, WO 2004032836 and in the articles JACS, 126, 3048 (2004) and JACS, 126, 9918 (2004).
Unless otherwise noted, all nonaqueous reactions were carried out under argon atmosphere with commercial dry solvents. Compounds were purified using flash column chromatography using Merck silica gel 60 (230-400 mesh) or reverse phase preparative HPLC using a Reprosil-Pur ODS3, 5 μm, 20 x 125 mm column or XTerra MS C18, 3.5 μm, 2.1 x 100 mm with Shimadzu LC8A-Pump and SPD-10Avp UV/Vis diode array detector. The 1H-NMR spectra were recorded on a Varian VXR-S (300 MHz for 1H-NMR) using d6-dimethylsulfoxide as solvent; chemical shifts are reported in ppm relative to tetramethylsilane. Analytical LC/MS was performed using: a) Reprosil- Pur ODS 3.5 μM, 1 x 60 mm column with a linear gradient acetonitrile in water (0.1% TFA) at a flow rate of 250 μL/min; b) XTerra MS C18, 3.5 μm, 2.1 * 100 mm, linear gradient with acetonitrile in water (0.1% HCOOH) at a flow rate of 250 μL/min; retention times are given in minutes. Methods are:
(I) linear gradient from 5% to 95% acetonitrile in water (0.1 % TFA); LC10Advp-Pump (Shimadzu) with SPD-M10Avp UV/Vis diode array detector and QP2010 MS-detector in ESI+ modus with UV-detection at 214, 254 and 275 nm, 5 min. linear gradient; (II) idem but 3 min. linear gradient; (III) linear gradient from 5% to 95% acetonitrile in water (0.1% TFA); runs on a LC10Advp-Pump (Shimadzu) with SPD-10Avp dual wavelength UV-detector and QP2010 MS-detector in ESI+ modus with UV-detection at 214 and 254 nm, 5 min. linear gradient; (IV) idem but 10 min. linear gradient; (V) linear gradient from 1% to 30% acetonitrile in water (0.1% TFA); (VI) linear gradient from 1 % to 60% acetonitrile in water (0.1% TFA). General procedure for making compounds of the invention
In general, compounds having the structure (I)
Figure imgf000038_0001
wherein the variables have the above described meanings, may be prepared in the case of X = C(O) by standard peptide coupling conditions. For example, it may be possible to use 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) hydrochloride in combination with 1 -hydroxybenzotriazole (HOBt) and a base (triethylamine or diisopropylethylamine) or 0-(7-azabenzotriazol-1-yl)-Λ/;Λ/,/\/',Λ/-tetramethyluronium hexafluorophosphate (HATU) or 0-(7-benzotriazol-1-yl)-Λ/,A/,Λ/' Λ/-tetramethyluronium hexafluorophosphate (HBTU) in the presence of a base, in solvents such as methylene chloride, Λ/,N-dimethylformamide..or dimethylsulfoxide. In the case of X = CR13R14 by reductive amination conditions, for example, it may be possible to use sodium triacetoxyborohydride in acidic media (such as in solvents like methanol or dichloromethane containing acetic acid) in combination with a suitable amine and suitable aldehyde. In the case of X = CR13R14 by reduction of an amide carbonyl C(O) group to the corresponding carbon, the amide, prepared as described above, may be reduced with lithium aluminiumhydride in solvents such as tetrahydrofuran. In the case of X = CR13R14 by displacement of a suitable leaving group by a suitable amine, the leaving group may be a tosylate, halide, triflate or mesylate reacting with the amine in solvents such as dichloromethane, acetonitrile or Λ/,Λ/-dimethylformamide, in the presence of a base such as triethylamine or 2,6-lutidine, for example. In the case of X = S(0)2 this may be prepared by coupling of a suitably prepared sulfonyl chloride with a suitable amine following standard sulfonylation conditions, in solvents such as dichloromethane in the presence of a base such as triethylamine or 2,6-lutidine. In the case of X = S(O) by coupling of a suitable amine with a suitable sulfinyl halide in solvents such as diethylether.
Scheme H outlines a procedure for using the amines of formula (III) and sulphonic acid chlorides of formula (lib) to synthesize preferred compounds that are embodiments of the invention. Scheme H
7 cuouupμlπiin ιyg reagents Step l
Figure imgf000039_0002
Figure imgf000039_0001
Figure imgf000039_0003
Scheme I outlines a procedure for using solid phase synthesis to synthesize preferred compounds that are embodiments of the invention.
Scheme I θγcy
Figure imgf000039_0004
Scheme J outlines a procedure for using the amines of formula (III) and acids of formula (lla) to synthesize preferred compounds that are embodiments of the invention. Scheme J
Figure imgf000040_0001
The protective group may be removed with, for example, diethylamine in dichloromethane in the case of Fmoc or using acidic conditions (such as trifluoroacetic acid in dichloromethane or hydrochloric acid in dioxane) in the case of Boc, as described in Protective Groups in Organic Synthesis. 3rd ed., Ed. Wiley-VCH, New York; 1999.
For the purification of intermediates or end products, flash chromatography on silica gel may be suitable for the isolation of free amines whereas the use of preparative HPLC leads to the isolation of the corresponding trifluoroacetic acid salts.
Compounds may be prepared by other means, however, and the suggested starting materials and procedures described below are exemplary only and should not be considered as limiting the scope of the invention.
EXAMPLES
The following examples are provided so that the invention might be more fully understood. These examples are illustrative only and should not be construed as limiting the invention in any way. PREPARATIONS
Example 1
Figure imgf000041_0001
Procedure for making an intermediate according to Scheme A.
Figure imgf000041_0002
(2-Fluoro-benzvO-methyl amine. .
To a solution of 300 mg (2.40 mmol) of 2-fluorobenzylamine, 29.3 mg (0.24 mmol) of 4- dimethylaminopyridine and 342 μl (2.64 mmol) of triethylamine in 3 mL of dichloromethane is added 628 mg (2.88 mmol) of di(tert-butoxycarbonyl) in 3 mL of dichloromethane at 0 °C. The reaction is allowed to react for 72 h and solvents are removed under reduced pressure. The crude product is dissolved in 5 mL of 1 N hydrochloric acid solution and extracted three times with ethyl acetate. The organic phase is separated and washed with saturated sodium bicarbonate solution and brine and dried with sodium sulphate. Removal of the solvent affords (2-fluoro-benzyl)- carbamic acid tetf-butyl ester, which is taken directly to the next step.
To a solution of 250 mg (1.11 mmol) of the carbamic ester dissolved in 1 mL of tetrahydrofuran is added 1.66 mL of a 1 M lithium aluminiumhydride solution in tetrahydrofuran. The reaction mixture is stirred at room temperature until gas evolution has ceased and is further heated at reflux temperature for 3 h. After cooling to room temperature, 1 N hydrochloric acid solution is added and the aqueous phase is extracted with dichloromethane. The aqueous phase is separated, a saturated sodium bicarbonate solution is added until pH reaches 7-8 and dichloromethane is added. The organic layer is extracted with brine and water, dried with sodium sulphate and concentrated under vacuum to obtain the title compound. LC/MS (III) rt 1.52, m/z 140 [M+H]+.
The compounds in Table 1 are synthesized according to the procedure shown for example 1.
Table 1
Figure imgf000042_0001
Figure imgf000043_0004
Figure imgf000043_0001
Procedure for making an intermediate according to Scheme B.
Step l
1) EtOH, reflux
Figure imgf000043_0002
Figure imgf000043_0003
(f?)-1-Phenyl-2-pyrrolidin-1-yl-ethylamine. (For synthesis, see Tetrahedron Lett. 1996;
37; 5619-5622.)
A mixture of 100.0 mg (0.83 mmol) of (RJ-styrene oxide and 94.7 mg (1.33 mmol) of pyrrolidine are dissolved in 5 mL ethanol and heated under reflux for 3 h. After cooling, the solvent is evaporated under reduced pressure to give the crude product which is thoroughly dried for at least 1 h under vacuum. Under nitrogen, this crude product is dissolved in 5 mL ether, 255 μL (2.5 mmol) triethylamine is added and the solution is cooled to 0°C. Then, 97 μL (1.25 mmol) methansulfonyl chloride is added dropwise.
After being allowed to warm to room temperature, 2 mL ammonium hydroxide and 4 mL THF are added and the resulting reaction mixture is vigorously stirred for 16 h. The solvent is evaporated under reduced pressure and the crude mixture is purified by flash chromatography on silica gel with dichloromethane/methanol (10% ammonium hydroxide).
1H NMR (d6-DMSO, 200 MHz) δ = 1.67 (m, 4H), 2.28- 2.60 (m, 6H), 3.93-3.97 (m,1 H),
7.14-7.34 (m, 5H). LC/MS (Via) rt 0.26, m/z 191 [M+H]+.
The compounds in Table 2 are synthesized according to the procedure shown for example 10.
Table 2
Figure imgf000044_0001
Figure imgf000045_0003
Example 17
Figure imgf000045_0001
Procedure for making an intermediate according to Scheme C.
Step l
Figure imgf000045_0002
(3-Chloro-phenyl)-pyrrolidin-1-v,-acetonitrile.
202 mg (1.42 mmol) sodium sulfite and 43.6 μL (1.42 mmol) of cone, hydrochloric are dissolved in 14 mL water and 200 mg (1.42 mmol) of 3-chlorobenzaldehyde are added subsequently. After stirring for 15 min at room temperature, 130 μL (1.57 mmol) pyrrolidine are added and stirring continued for another 30 min. The reaction mixture is cooled to 0°C and 70 mg (1.42 mmol) sodium cyanate, dissolved in 7 mL water, are added dropwise. The mixture is allowed to warm up to room temperature and stirred vigorously overnight. Diethylether is added, the organic layer separated and the aqueous layer is extracted twice with diethylether. The combined extracts are dried over sodium sulphate and the solvent is evaporated under reduced pressure. The crude product is purified by flash chromatography (cyclohexane/ethylacetat 5:1 ), yielding the title compound as a colorless oil. LC/MS (Va) rt 5.75, m/z 221 [M+H]+.
Step 2
Figure imgf000046_0001
2-(3-Chloro-phenyl)-2-pyrrolidin-1-yl-ethylamine.
183 mg (0.83mmol) (3-Chloro-phenyl)-pyrrolidin-1-yl-acetonitrile are dissolved in 8 mL ethanol and 1.6 mL cone, ammonia, a slurry of Raney-Nickel in water is added and the mixture is exposed to hydrogen (1 atmosphere) for 16 h. The catalyst is removed by filtration through celite and the is evaporated under reduced pressure. Purification of the crude product by filtration through a plug of silicagel (DCM/MeOH 9:1 as eluent) yields the title compound.
LC/MS (Va) rt 5.75, m/z 225 [M+H]+.
The compounds in Table 3 are synthesized according to the procedure shown for example 17. Table 3
Figure imgf000047_0002
Example 22
Figure imgf000047_0001
Procedure for making an intermediate according to Scheme D. Step 1
Figure imgf000048_0001
(2-Methylsulphanyl-benzylVcarbamic acid tert-butyl ester.
To a solution of 153.0 mg (1.00 mmol) of 2-methylsulphanyl-benzylamine, 12.2 mg (0.10 mmol) of 4-dimethylaminopyridine and 11.1 mg (1.10 mmol) of triethylamine in 3 mL of dichloromethane is added 262 mg (1.20 mmol) of di(tert-butyloxycarbonyl) in 3 mL of dichloromethane at 0 °C. The reaction is allowed to react during 16 h and solvents are removed under reduced pressure. The crude product is dissolved in 5 mL of 1N hydrochloric acid solution and extracted three times with ethyl acetate. The organic layer is separated and washed with saturated sodium bicarbonate solution and brine and dried with sodium sulphate. Removal of the solvent affords the crude product. The residue is purified using flash chromatography (silica gel, eluent: 0% to 25% ethyl acetate in cyclohexane) to afford the title compound. LC/MS (la) rt 2.97, m/z 239 (M-(CH3))+; 154 (M-boc) +.
Step 2
Nal04, THF, water
Figure imgf000048_0002
Figure imgf000048_0003
(2-Methanesulphinyl-benzyl)-carbamic acid tert-butyl ester.
To a solution of 50.0 mg (0.16 mmol) of (2-methylsulphanyl-benzyl)-carbamic acid tert- butyl ester in 1 ml THF are added dropwise 101.3 mg (0.47 mmol) of sodium periodate dissolved in 500 μL water. The reaction is allowed to react during 16 h and solvents are removed under reduced pressure. The residue is purified using flash chromatography (silica gel, eluent: 0% to 5% methanol in dichloromethane) to afford the title compound. LC/MS (la) rt 2.14, m/z 270 [M+H]+. Step 3
Figure imgf000049_0001
2-Methanesulphinyl-benzylamine.
30 mg (0.06 mmol) of the product from step 3 are dissolved in 2 mL dichloromethane and 1 mL of trifluoroacetic acid is added. The mixture is stirred for 30 min. and the solvent is evaporated in vacuum. The crude product is used in the next step without further purification.
LC/MS (la) rt 1.82, m/z 169 [M-boc]+.
Example 23
Figure imgf000049_0002
Step l
Figure imgf000049_0003
5-chloro-2-[1 ,2,41triazol-1 -yl-benzonitrile (For synthesis, see J. Med. Chem.; 47; 2004; 2995-3008)
To a solution of 2,5-dichlorobenzonitrile (1.00 g, 5.81 mmol) in mL N,N- dimethylformamide (10 mL) are added cesium carbonate (2.27 g, 6.98 mmol) and 1,2,4-triazole (482 mg, 6.98 mmol). The reaction mixture is stirred at 85 °C for 16 h and at 100 °C for 8 h. The reaction mixture is diluted with water and extracted with ethyl acetate. The combined organic layers are washed with aqueous lithium chloride, dried, and concentrated to give 5-chloro-2-[1 ,2,4]triazol-1-yl-benzonitrile (1.12 g, 5.47 mmol). The crude product is used in the next step without further purification. LC/MS (la) rt 3.00, 205 [M+H]+.
Step 2
Figure imgf000050_0001
5-chloro-2-H ,2,41triazol-1 -yl-benzylamine
A suspension of 5-chloro-2-[1 ,2,4]triazol-1-yl-benzonitrile (500 mg, 2.42 mmol) in ethanol saturated with ammonia (20 mL) is stirred in the presence of Raney nickel
(50% slurry in water, washed with ethanol, catalytic amount) under a hydrogen atmosphere for 26 h. The reaction mixture is filtered over Celite and concentrated.
Purification by flash chromatography (silica gel, eluent = 2% to 10 % DCM (with 10% ammonium hydroxide) in methanol) gives 5-chloro-2-[1,2,4]triazol-1-yl-benzylamine.
1H NMR (d6-DMSO, 200 MHz) δ = 3.54 (s, 2H), 7.43 (m, 2H), 7.73 (m,1H), 8.18 (s,
1 H), 8.60 (s, 1 H).
LC/MS (la) rt 2.06, 209 (M). Example 24
Figure imgf000051_0001
Step l
Figure imgf000051_0002
5-chloro-2-amino-α-chloroacetophenone
To a stirred solution of boron trichloride (8.62 mL of 1 M solution in heptane) in dry benzene (5 mL), a solution of 4-chloroaniline (1.00 g, 7.84 mmol) in dry benzene (15 mL) is added dropwise under icecooling. To the resulting mixture containing 4-chloroaniline borontrichloride complex, chloroacetonitrile (0.60 mL, 9.41 mmol) and aluminiumtrichloride (1.15 g, 8.62 mmol) are added successively. The mixture is then refluxed for 6 h under nitrogen, becoming a solution of two layers. The evolved hydrogen chloride is absorbed through a drying tube containing silica gel or calcium chloride to a surface of aqueous sodium hydroxide. After cooling with ice, 2 N hydrochloric acid is added and a yellow precipitate is formed. To hydrolyze the ketimine of 5-chloro-2-amino-a-chloroacetophenone, the mixture is warmed at 80 °C under stirring, until the precipitate has dissolved (ca. 30 min). The cooled mixture is extracted with chloromethane (three times) and the organic layer is washed with water, dried with sodium sulphate, and concentrated. The neutral fraction obtained (1.00 g) is recrystallized to obtain the pure 5-chloro-2-amino-α-chloroacetophenone. LC/MS (la) rt 2.77, 245 (M+H+AcCN)+.
Step 2
Figure imgf000051_0003
5-chloro-3-(chloromethvB-1 H-indazole
To a stirred suspension of 2-amino-5-chloro-α-chloroacetophenone (670 mg, 3.28 mmol) in cone, hydrochloric acid (10 mL) is added a solution of sodium nitrite (250 mg, 3.61 mmol) in water (2 mL) while mantaining the reaction temperature at 0 °C. After 1 h a solution of SnCI2»2H20 (1.78 g, 7.87 mmol ) in cone, hydrochloric acid (5 mL) is added to the reaction mixture, which is then stirred at the same temperature for 1 h. Next, ice water is added to the reaction mixture. The precipitate is collected by filtration, washed with water and dried giving crude 5-chloro-3-(chloromethyl)-1 H-indazole which is used in the next step without further purification. LC/MS (la) 2.67, no mass peak.
Step 3
Figure imgf000052_0001
3-(Azidomethyl)-5-chloro-1 H-indazole.
A stirred solution containing 5-chloro-3-(chloromethyl)-1 H-indazole (370 mg, 1.84 mmol), sodium azide (156 mg, 2.40 mmol), water (0.5 mL) and Λ/,Λ/-dimethylformamide
(5.00 mL) is warmed at 90 °C for 1 h and then the mixture is concentrated under reduced pressure. Ice is added and the resulting precipitate is collected by filtration and washed with water giving the 3-(azidomethyl)-5-chloro-1 H-indazole.
LC/MS (la) rt 2.63, 249 (M+H+AcCN)+.
Step 4
Figure imgf000052_0002
3-(aminomethyl)-5-chloro-1 H-indazole
To a stirred 1 M THF-solution of Lithium aluminium hydride (5.00 mL) is added a solution of 3-(azidomethyl)-5-chloro-1 H-indazole (330 mg ,1.59 mmol) in Et20 (10 mL) dropwise at room temperature, and the mixture is refluxed for 1 h. After quenching the excess of Lithium aluminium hydride with wet diethyl ether, the precipitate is filtered off and washed with dichloromethane:ethanol (9:1 ), giving crude 3-(aminomethyl)-5- chloro-1 H-indazole. The purification by column chromatography (silica gel, eluent =
10% DCM in methanol with 0.1 % Et3N) affords the pure material.
1H NMR (d6-DMSO, 200 MHz) δ = 4.01 (s, 2H), 7.25-7.28 (m, 1 H), 7.43-7.47 (m,1 H),
7.92-7.93 (m, 1H).
LC/MS (la) rt 1.59, 182 [M+H]+.
Example 25
Figure imgf000053_0001
Procedure for making an intermediate according to Scheme F.
Step 1
Figure imgf000053_0002
(3-Cvclopropylcarbamoyl-benzyl)-carbamic acid ferf-butyl ester
To a solution of 77 mg (0.62 mmol) pyridine-2-carboxylic acid in 2 mL N,N- dimethylformamide are added 156 mg (0.81 mmol) 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride (EDC), 110 mg (0.81 mmol) 1 -hydroxybenzotriazole (HOBt) and 155 μL (0.94 mmol) diisopropylethylamine (DIEA) and after 10 min 100 mg (0.62 mmol) (2-amino-ethyl)-carbamic acid fetf-butyl ester. The mixture is stirred overnight at ambient temperature and diluted with ethyl acetate. The organic phase is washed sequentially with 5 % citric acid, saturated aqueous sodium bicarbonate solution, and brine, dried over sodium sulphate and concentrated under vacuum to yield the title compound.
LCMS (la) rt 3.51 , m/z 266 [M+H]+.
Step 2
Figure imgf000054_0001
3-Aminomethyl-N-cvclopropyl-benzamide
30 mg (0.10 mmol) of the product from step 1 are dissolved in 2 mL dichloromethane and 1 mL of trifluoroacetic acid is added. The mixture is stirred for 30 min and the solvent is evaporated under vacuum. The title compound is taken directly to the next step.
1H NMR (d6-DMSO, 300 MHz) δ = 2.71 (m, 2H), 3.31 (m, 2H), 7.52-7.56 . (m, 1 H),
7.91-8.00 (m, 2H), 8.58-8.59 (m, 1 H).
Example 26
Figure imgf000054_0002
Step l
Figure imgf000054_0003
2-PyrazoH -yl-benzonitrile.
To a solution of 200 mg (1.65 mmol) 2-fluoro-benzonitrile and 135 mg (1.98 mmol) pyrazole dissolved in 2 mL Λ/,Λ/-dimethylformamide is added 274 mg (1.98 mmol) potassium carbonate. The mixture is stirred overnight under argon at 120°C and diluted with ethyl acetate. The organic phase is washed sequentially with water and brine, dried over sodium sulphate and concentrated under vacuum to yield the title compound.
LCMS (IVa) rt 3.31 , m/z 170 [M+H]+.
Step 2
Figure imgf000055_0001
2-Pyrazol-1-yl-benzylamine.
A suspension of 2-pyrazol-1-yl-benzonitrile (126 mg, 0.74 mmol) in ethanol saturated with ammonia (5 mL) is stirred in the presence of Raney nickel (50% slurry in water, washed with ethanol, catalytic amount) under a hydrogen atmosphere for 66 h. The reaction mixture is filtered over Celite and concentrated. Purification of the crude product by filtration through a plug of silicagel (dichloromethane:methanol 9:1 as eluent) yields the title compound. LC/MS (la) 11 1.77, 174 [M+H]+.
Example 27
Figure imgf000055_0002
3-Pyrazol-1 -yl-benzylamine.
The title compound was prepared according the procedure from example 26.
LCMS (Va) rt 4.49, m/z 174 [M+H]+. Example 28
Figure imgf000056_0001
Step l
Figure imgf000056_0002
4-Pyrazol-1 -yl-benzonitrile.
The title compound was prepared according the procedure from step 1 example 26.
LCMS (IVa) rt 3.78, m/z 170 [M+H]+. '
Step 2 NaBH4, isobutyric acid, N≡— y-Λn J eLi' : THF ^ V >—_Γ </ VMN H2N
1 -(4-Pyrazol-1 -yl-phenvD-ethylamine.
A suspension of sodium borohydride (22.3 mg, 0.59 mmol) in tetrahydrofuran (5 mL) is stirred at room temperature. Isobutyric acid (54.8 μL, 0.59 mmol) and 4-pyrazol-1-yl- benzonitrile (100 mg, 0.591 mmol), dissolved in 3 mL tetrahydrofuran, are added. The reaction mixture is stirred at 70°C for 6 h and then cooled to -80°C. Methyllithium (739 μL, 1.18 mmol, 1.6 M in diethyl ether) is added and the reaction mixture is allowed to warm to room temperature over night. The reaction is quenched by addition of 1 mL water and 2 mL 1 M HCl. The organic solvent is evaporated under reduced pressure and the remaining aqueous phase is adjusted with ammonium hydroxide to pH = 7 and extracted with ethylacetate (three times) and the combined organic layer is washed with water, dried with sodium sulphate, and concentrated in vacuum. Purification of the crude product by preparative HPLC yields the title compound.
1H NMR (d6-DMSO, 300 MHz) δ = 1.52 (d, 3H, J = 6.9 Hz), 4.43 (m, 1 H), 6.51 (m,1 H), 7.56 (d, 2H, J = 9.0 Hz), 7.70 (s, 1 H), 7.85 (d, 2H, J = 9.0 Hz), 8.46 (d, 1 H, J = 2.7 Hz). LC/MS (Via) rt 3.63, 187 (M).
Example 29
Figure imgf000057_0001
Procedure for making an intermediate according to Scheme G and a compound of the invention according to Scheme J.
Step 1
Figure imgf000057_0002
Λ/-Hvdroxy-cvclopropanecarboxamidine
1.00 g (14.9 mmol) of cyclopropanecarbonitrile is dissolved in 20 mL of methanol, 3.09 g (22.4 mmol) of powdered potassium carbonate is added and 2.07 g (29.8 mmol) of hydroxylamine hydrochloride is added subsequently in small portions. The reaction mixture is refluxed for 5 h, then the solvent is evaporated and the residue is taken up in a 1 :4 mixture of water and chloroform. The organic layer is separated, washed twice with water, dried with magnesium sulphate, filtered and evaporated to dryness. The residue is recrystallized from ether/hexane to afford the desired product. Steps 2 and 3
Figure imgf000058_0001
r(S)-1-(3-Cvclopropyl-ri ,2.41oxadiazol-5-yl)-2-methyl-propyn-carbamic acid tert-butyl ester
To a solution of 50.0 mg (0.50 mmol) Λ/-hydroxy-cyclopropylcarboxamidine, 109 mg (0.50 mmole) Boc-(S)-valine in 2 mL dichloromethane, 75.5 mg (0.60 mmol) 1 ,3-di-/so- propylcarbodiimide is added, and the mixture is stirred at room temperature. After 12 h the solvent is evaporated and the residue is dissolved in 10 mL of pyridine and refluxed for 4 h. Then the pyridine is evaporated and the residue is taken up in a 2:1 mixture of dichloromethane and water. The aqueous phase is extracted with dichloromethane, and the combined organic layers are washed with 3 % hydrochloric acid solution, saturated sodium bicarbonate solution, water and brine, dried with sodium sulphate, filtered and concentrated in vacuo. The residue is subjected to column chromatography (silica gel, eluent: c-hexane:ethyl acetate = 50:50) to afford the title compound. 1 H-NMR (300 MHz, DMSO-d6) δ = 0.78-0.91 (m, 8H), 1.04-1.07 (m, 2H), 1.22-1.37 (d, 9H), 2.08-2.12 (m, 2H), 4.52 (m, 1 H), 7.65-7.67 (d, 1 H, NH). LCMS (la) rt 4.21 , 226, 267, 282.
Step 4
Figure imgf000058_0002
(S)-1-(3-Cyclopropyl-H ,2,41oxadiazol-5-v0-2-methyl-propylamine trifluoroacetic acid salt
85.0 mg (0.30 mmol) of [(S)-1-(3-cycIopropyl-[1 ,2,4]oxadiazol-5-yl)-2-methyl-propyl]- carbamic acid ferf-butyl ester from step 3 is dissolved in 2 mL dichloromethane and the mixture is cooled to 0°C. Then 1 mL of trifluoroacetic acid is added and the reaction solution is allowed to warm to room temperature. After complete conversion of the starting material, as monitored by TLC the solvent is evaporated and the residue is dried in vacuo to yield the title compound.
Step 5
Figure imgf000059_0001
r(ffl-1-(r(S)-1-(3-Cvclopropyl-ri ,2,4loxadiazol-5-yl)-2-methyl-propylcarbamovn-methyl)- 2-(2,4,5-trifluoro-phenyl)-ethyll-carbamic acid ferf-butyl ester
A mixture of 99.0 mg (0.30 mmol) Boc-(f?)-3-amino-4-(2,4,5-trifluoro-phenyl)-butyric acid, 60.0 mg (0.45 mmol) 1 -hydroxybenzotriazole, 183 μL (1.31 mmol) triethylamine and 86.0 mg (0.45 mmol) of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride in 4 mL of Λ/,Λ/-dimethylformamide is stirred for 10 min. Then 89.0 mg (0.30 mmol) of the trifluoroacetic acid salt of (S)-1-(3-cyclopropyl-[1 ,2,4]oxadiazol-5-yl)- 2-methyl-propylamine is added to the reaction mixture. After stirring over night the solution is poured into 15 mL of brine and the resulting mixture is extracted 3 times with ethyl acetate. The combined organic layers are washed with saturated sodium bicarbonate solution, water and brine, dried with sodium sulphate, filtered and concentrated in vacuo. Purification by flash chromatography on silica gel (c- hexane:ethyl acetate 50:50) affords the title compound. LCMS (la) rt 4.55, 397 (M-boc)+, 438 (M-boc+CH3CN)+, 497 (M+H)+. Step 6
Figure imgf000060_0001
(ffl-3-Amino-Λ/-r(S)-1-(3-cvclopropyl-π .2.4loxadiazol-5-yl)-2-methyl-propyn-4-(2.4.5- trifluoro-phenvD-butyramide. formic acid salt
108 mg (0.22 mmol) of [(f?)-1-{[(S)-1-(3-cyclopropyl-[1,2,4]oxadiazol-5-yl)-2-methyl- propylcarbamoyl]-methyl}-2-(2,4,5-trifluoro-phenyl)-ethyl]-carbamic acid tert-butyl ester is dissolved in 1 mL dichloromethane and cooled to 0°C. Then 0.5 mL of trifluoroacetic acid is added and the reaction mixture is allowed to warm to room temperature over the course of the reaction. Stirring is continued until complete conversion is observed by TLC analysis. The solvents are evaporated under vacuo and the residue is subjected to purification by preparative HPLC affording the title compound.
1H-NMR (300 MHz, DMSO-d6) δ = 0.80-0.82 (d, 5H), 0.90-0.92 (d, 3H), 1.04-1.06 (m, 2H), 2.07-2.12 (m, 2H), 2.83-2.87 (m, 2H), 3.63-3.65 (m, 1 H), 4.83-4.86 (m, 1 H), 7.43- 7.47 (t, 1 H), 7.50-7.57 (t, 1 H), 8.14 (s, 1 H), 8.84-8.86 (d, 1 H, NH). LCMS (la) rt 2.54, 397 (M+H)+, 438 (M+CH3CN)+.
Example 30
Figure imgf000060_0002
Procedure for making a compound of the invention according to Scheme
Figure imgf000061_0001
3-(r(3f?)-3-Amino-4-(2-fluoro-phenyl)-butyrylaminol-methyl)-benzamide.trifluoroacetate salt
40 mg Rink Amide Novagel (loading 0.6 mmol/g - 0.024 mmol) are briefly swollen in Λ/;Λ/-dimethylformamide and drained. A solution of 45 mg (0.11 mmol) Fmoc-(3- aminomethyl)-benzoic acid and 21 μL (0.15 mmol) diisopropylcarbodiimide (DIC) in 0.5 mL Λ/,Λ/-dimethylformamide is added and the reaction is shaken at ambient temperature for 5 hours. The solution is drained and the resin is washed with N,N- dimethylformamide (3x), dichloromethane (2x), methanol (2x), dichloromethane (2x) and diethylether (2x). The fluorenylmethoxycarbonyl (Fmoc) group is then removed by treatment with 20% piperidine in Λ/,Λ/-dimethylformamide, shaken for 30 min, followed by washing with Λ/,Λ/-dimethylformamide (3x). A solution of 15 mg (0.052 mmol) Boc- (R)-3-amino-4-(2-fluoro-phenyl)-butyric acid, 9 μL (0.057 mmol) diisopropylcarbodiimide (DIC), 7.5 mg (0.057 mmol) 1 -hydroxybenzotriazole (HOBt) and 15 μL (0.092 mmol) diisopropylethylamine (DIEA) in 0.5 mL DMF is added and the mixture is shaken overnight. The resin is then washed with Λ/,Λ/-dimethylformamide (3x), dichloromethane (2x), methanol (2x), dichloromethane (2x) and diethylether (2x) and dried. Cleavage from the resin is performed by treatment with 95% trifluoroacetic acid in DCM for 90 min, followed by washing of the resin with 95% trifluoroacetic acid in DCM (2x). Evaporation of the solvent gives crude material that is purified by reversed phase HPLC yielding the title compound. LCMS (Ilia) rt 2.07, m/z 330 (M+H)+.
Example 31
Figure imgf000062_0001
4-(r(3R)-3-Amino-4-(2-fluoro-phenyl)-butyrylaminol-methyl)-benzamide.trifluoroacetate salt
The title compound was prepared according to the procedure from example 30.
LCMS (Ilia) rt 2.08, m/z 330 (M+H)+.
Example 32
Figure imgf000062_0002
Procedure for making an intermediate according to Scheme E and a compound of the invention according to Scheme J.
Step l
Figure imgf000062_0003
(3-Cyclopropylcarbamoyl-benzyl)-carbamic acid tert-butyl ester
To a solution of 30 mg (0.12 mmol) Boc-(3-aminomethyl)-benzoic acid in 3 mL N,N- dimethylformamide is added 25 mg (0.13 mmol) 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride (EDC), 18 mg (0.13 mmol) 1 -hydroxybenzotriazole (HOBt) and 49 μL (0.29 mmol) diisopropylethylamine (DIEA) and after 10 min 8 mg (0.14 mmol) cyclopropylamine. The mixture is stirred overnight at ambient temperature and diluted with ethyl acetate. The organic layer is washed sequentially with 5 % citric acid, saturated aqueous sodium bicarbonate solution and brine, dried over sodium sulphate and concentrated under vacuum to yield the title compound. LCMS (la) rt 2.31 , m/z 313 (M+Na)+.
Step 2
Figure imgf000063_0001
3-Aminomethyl-Λ/-cyclopropyl-benzamide. trifluoroacetate salt
30 mg (0.10 mmol) of the product from step 1 are dissolved in 2 mL dichloromethane and 1 mL of trifluoroacetic acid is added. The mixture is stirred for 30 min and the solvent is evaporated in vacuum. The title compound is directly taken to the next step. LCMS (la) rt 1.52, m/z 191 (M+H)+.
Step 3
Figure imgf000063_0002
r(ffl-1-f(3-Cvclopropylcarbamoyl-benzylcarbamoyl)-methvn-2-(2-fluoro-phenvn-ethvn- carbamic acid tert-butyl ester
To a solution of 55 mg (0.19 mmol) Boc-(/:?)-3-amino-4-(2-fluoro-phenyl)-butyric acid in 2 mL Λ/,Λ/-dimethylformamide is added 39 mg (0.20 mmol) 1 -ethyl-3-(3- dimethylaminopropyl)-carbodiimide hydrochloride (EDC), 33 mg (0.24 mmol) 1- hydroxybenzotriazole (HOBt) and 75 μL (0.44 mmol) diisopropylethylamine (DIEA). After 10 min the crude material from step 2 (0.10 mmol) is dissolved in 1 mL N,N- dimethylformamide and added to the reaction mixture. The mixture is stirred overnight at ambient temperature and diluted with ethyl acetate. The organic phase is washed sequentially with 5 % citric acid, saturated aqueous sodium bicarbonate solution and brine, dried over sodium sulphate and concentrated under vacuum. Purification by flash chromatography (silica gel, cyclohexane to ethyl acetate) affords the title compound. LCMS (Ilia) rt 2.65, m/z 492 (M+Na)+.
Step 4
Figure imgf000064_0001
3-{r(3f?)-3-Amino-4-(2-fluoro-phenyl)-butyrylamino1-methyl)-Λ/-cyclopropyl-benzamide. trifluoroacetate salt
30 mg (0.06 mmol) of the product from step 3 are dissolved in 2 mL dichloromethane and 1 mL of trifluoroacetic acid is added. The mixture is stirred for 30 min and the solvent is evaporated in vacuum. The crude material is purified by reversed phase HPLC to yield the title compound. 1H-NMR (300 MHz, DMSO-d6) δ = 0.57 (m, 2H), 0.70 (m, 2H), 2.50 (m, 2H covered by dmso signal), 2.81 - 2.90 (m, 2H), 3.00 (dd, 1 H, J = 13.1 Hz, J = 5.1 Hz), 3.69 (m, 1 H), 4.27 (m, 2H), 7.13 - 7.33 (m, 6H), 7.66 (m, 2H), 7.96 (bs, 3H), 8.30 (d, 1 H, J = 3.8Hz), 8.58 (t, 1 H, J = 5.1 Hz). LCMS (la) rt 2.02, m/z 370 (M+H)+.
The compounds in Table 4 are synthesized according to the procedure shown for example 32. Table 4
Figure imgf000065_0001
Figure imgf000066_0002
The following examples deal with compounds of the invention synthesized according to Scheme J. A description of the general procedures used for Steps 1 and 2 follows.
Example 36
Step l
Figure imgf000066_0001
π-(f?)-(r(2-Fluoro-benzyl)-methyl-carbamoyll-methyl}-2-(2-fluoro-phenvπ-ethyll- carbamic acid tert-butyl ester. A mixture of 20.0 mg (0.07 mmol) of (3R)-tert-butoxycarbonylamino-4-[2-fluoro-phenyl]- butyric acid, 12.8 mg (0.09 mmol) of 1 -hydroxybenzotriazole, 18.1 mg (0.09 mmol) of 1- ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and 38 μL (0.29 mmol) of diisopropylethylamine in 1.5 mL of Λ/;Λ/-dimethylformamide is stirred for 5 min. After addition of 11.8 mg (0.08 mmol) of (2-fluoro-benzyi)-methyl amine (Example 1 ) in 0.5 mL of Λ/,/V-dimethylformamide, the mixture is stirred for further 16 h. The solution is diluted with 5 mL of 1 N hydrochloric acid solution and extracted twice with 10 mL of dichloromethane. The collected organic phases are washed with brine and water, dried over sodium sulphate and evaporated under reduced pressure. The residue is purified using flash chromatography (silica gel, eluent: 0% to 20% ethyl acetate in cyclohexane) to afford the title compound. LC/MS (la) rt 3.24, m/z 419 [M+H]+.
Step 2
Figure imgf000067_0001
f3f?)-Amino-Λ/-(2-fluoro-benzyl)-4-(2-fluoro-phenyl)-Λ/-methyl-butyramide hydrochloride.
A solution of 20.0 mg (0.04 mmol) of [1-(ftK[(2-fluoro-benzyl)-methyl-carbamoyl]- methyl}-2-(2-fluoro-phenyl)-ethyl]-carbamic acid tert-butyl ester in 1.0 mL of 4N hydrochloric acid solution in 1 ,4-dioxan is stirred for 2 h and then evaporated under reduced pressure. The crude mixture is diluted in methanol and again evaporated under vacuum to afford the title compound.
1H-NMR δ (ppm) = 2.65-2.88 (m, 5H), 2.95-3.15 (m, 2H), 3.67 (m, 2H), 4.27-4.53 (2m, 1 H), 7.10-7.33 (m, 8H), 8.28 (bs, 2H). LC/MS (la) rt 2,13, m/z 319 [M+H]+.
Example 37
Figure imgf000067_0002
Example 37 prepared following the procedure above outlined for Example 36 according to Scheme J.
Step l
Figure imgf000068_0001
(Σ^Σ-Fluoro-phenvD-l-πmethyl^Σ-methyl-benzvD-carbamoyll-methvD-ethvD-carbamic acid tert-butyl ester.
Obtained from (3f?)-tert-butoxycarbonylamino-4-[2-fluoro-phenyl]-butyric acid and methyl-(2-methyl-benzyl)-amine.
LC/MS (la) rt 3.37, m/z 415 [M+H]+.
Step 2
Figure imgf000068_0002
3-Amino-4-(2-fluoro-phenyl)-A/-methyl-Λ/-(2-methyl-benzyl)-butyramide hydrochloride. Obtained from (2-(2-fluoro-phenyl)-1 -{[methyl-(2-methyl-benzyl)-carbamoyl]-methyl}- ethyl)-carbamic acid tert-butyl ester according to Step 2 in the procedure described for Example 36. LC/MS (la) rt 2.30, m/z 315 [M+H]+. Example 38
Figure imgf000069_0001
Example 38 prepared following the procedure outlined for Example 36 according to Scheme J.
Step l
Figure imgf000069_0002
(2-(2-Fluoro-phenyl)-1-{|'methyl-(2-methylsulfanyl-benzyl)-carbamoyll-methyl}-ethyl)- carbamic acid tert-butyl ester.
Obtained from 3R -tert-butoxycarbonylamino-4-[2-fluoro-phenyl]-butyric acid and methyl-(2-methylsulfanyl-benzyl)-amine.
LC/MS (la) rt 3.31 , m/z 415 [M+H]+.
Step 2
Figure imgf000069_0003
3-Amino-4-(2-fluoro-phenyl)-Λ/-methyl-Λ/-(2-methylsulfanyl-benzylVbutyramide hydrochloride.
Obtained from (2-(2-fluoro-phenyl)-1 -{[methyl-(2-methylsulfanyl-benzyl)-carbamoyl]- methyl}-ethyl)-carbamic acid tert-butyl ester according to Step 2 in the procedure described for Example 36.
LC/MS (la) rt 2.30, m/z 315 [M+H]+.
Compounds listed in Table 5 with the formula
Figure imgf000070_0001
are prepared according to the following experimental procedure:
Figure imgf000070_0002
To 100 μL of a 0.5M dimethylsulphoxide solution of 3R)-tert-butoxycarbonylamino-4- [2-fluoro-phenyl]-butyric acid in a well of a 96-MTP are added sequentially 140 μL of a 0.5M dimethylsulfoxide solution of 1 -hydroxybenzotriazole, 140 μL of a 0.5M dimethylsulfoxide solution of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and 20 μL (0.15 mmol) of diisopropylethylamine. After 15 min stirring at room temperature 0.06 mmol of the corresponding amine dissolved in 100 μL of dimethylsulfoxide is added and the reaction mixture is stirred overnight at room temperature. Solvents are removed under vacuum, 500 μL of a solution of trifluoroacetic acid in dichloromethane (33% v/v) is added and the reaction mixture is stirred for 2 h at room temperature. After removal of the solvents under reduced pressure, 500 μL of methanol are added and the crude material is purified using preparative HPLC with a 10 min linear gradient from 5% to 95% acetonitrile in water (0.1 % TFA) to afford the title compounds.
Diastereoisomeric compounds generated from racemic amines are separated via reversed phase HPLC. The absolute configuration for these diastereoisomeres is not assigned.
TABLE 5
Figure imgf000071_0001
Figure imgf000072_0001
Figure imgf000073_0001
Figure imgf000074_0001
Figure imgf000075_0001
Figure imgf000076_0001
Figure imgf000077_0001
Figure imgf000078_0001
Figure imgf000079_0001
Figure imgf000080_0001
Figure imgf000081_0001
Figure imgf000082_0001
Compounds listed in Table 6 with the formula
Figure imgf000083_0001
are prepared according to the following experimental procedure described in Scheme J:
Figure imgf000083_0002
To 100 μL of a 0.5M Λ/,Λ/-Dimethylformamide solution of (3R)-tert- butoxycarbonylamino-4-[2-fluoro-phenyl]-butyric acid in a well of a 96-MTP are added sequentially 140 μL of a 0.5M Λ/,Λ/-Dimethylformamide solution of 1- hydroxybenzotriazole, 140 μL of a 0.5M Λ/,Λ/-Dimethylformamide solution of 1-ethyl-3- (3-dimethylaminopropyl)carbodiimide hydrochloride and 20 μL (0.15 mmol) of diisopropylethylamine. After 15 min at room temperature 0.06 mmol of the corresponding amine dissolved in 100 μL of Λ/,Λ/-Dimethylformamide is added and the reaction mixture is stirred overnight at room temperature. Solvents are removed under vacuum, 500 μL of a solution of trifluoroacetic acid in dichloromethane (33% v/v) is added and the reaction mixture is stirred for 2 h at room temperature. After removal of the solvents under reduced pressure, 500 μL of methanol are added and the crude material is purified using preparative HPLC with a 10 min linear gradient from 5% to 95% acetonitrile in water (0.1 % TFA) to afford the title compounds. TABLE 6
Figure imgf000084_0001
(m,
(m,
Figure imgf000085_0001
Figure imgf000086_0001
Figure imgf000087_0001
Figure imgf000088_0001
Compounds listed in Table 7 with the formula
Figure imgf000088_0002
are prepared according to the following experimental procedure described in Scheme J:
Figure imgf000089_0001
To 26.4 mg (0.089 mmol) of (3/^-fert-butoxycarbonylamino-4-[2-fluoro-phenyl]-butyric acid dissolved in 1 mL Λ/,Λ/-DimethyIformamide are added 16.8 mg (0.124 mmol) of 1- hydroxybenzotriazole, 23.9 mg (0.124 mmol) 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride and 22 μL (0.124 mmol) of diisopropylethylamine. After 15 min stirring at room temperature 0.107 mmol of the corresponding amine are added and the reaction mixture is stirred overnight at room temperature. Solvents are removed in vacuum, 500 μL of a solution of trifluoroacetic acid in dichloromethane (33% v/v) are added and the reaction mixture is stirred for 2 h at room temperature. After removal of the solvents under reduced pressure, 500 μL of methanol are added and the crude material is purified using preparative HPLC with a 10 min linear gradient from 5% to 95% acetonitrile in water (0.1 % TFA) to afford the title compounds.
TABLE 7
Figure imgf000089_0002
Figure imgf000090_0001
Figure imgf000091_0001
Figure imgf000092_0001
Example 179
Figure imgf000092_0002
step 1
Figure imgf000092_0003
(R)-3-Amino-4-(2-fluoro-phenyl)-Λ/-methyl-N-(1-phenyl-2-pyrrolidin-1-yl-ethyl)- butyramide formic acid.
To 40.0 mg (0.13 mmol) of 3RJ-fert-butoxycarbonylamino-4-[2-fluoro-phenyl]-butyric acid dissolved in 3 mL Λ/,Λ/-Dimethylformamid including 1.0 eq of triethylamine are added 51.2 mg (0.13 mmol) of 0-(benzotrialzol-1-YL)-Λ/-Λ/-Λ/-Λ/-tetramethyluronium hexafluoro phosphate (HBTU). After 15 min. at room temperature 35.7 mg (0.176 mmol) of (R)-methyl-(1-phenyl-2-pyrrolidin-1-yl-ethyl)-amine and 37.2 μL (0.338 mmol) of Λ/-methylmorpholine are added and the reaction mixture is stirred overnight at 50°C. Solvents are removed under vacuum, 500 μL of a solution of trifluoroacetic acid in dichloromethane (33% v/v) is added and the reaction mixture is stirred for 2 h at room temperature. After removal of the solvents under reduced pressure, 500 μL of methanol are added and the crude material is purified using preparative HPLC with a 10 min linear gradient from 5% to 95% acetonitrile in water (0.1 % TFA) to afford the title compound. Diastereomeres generated from racemic amines are separated via reversed phase HPLC. The absolute configuration for these diastereomeres is not assigned.
The compounds listed in Table 8 are prepared according to the experimental procedure outlined for Example 179:
TABLE 8
(m, (m,
Figure imgf000093_0001
Figure imgf000094_0001
Figure imgf000095_0001
Example 191
Figure imgf000095_0002
Procedure for making a compound of the invention according to Scheme H.
Step l
Figure imgf000095_0003
(ffl-2-Amino-3-(2-fluoro-phenyl)-propionic acid methyl ester hydrochloride
1.50 g (5.11 mmol) of (R)-2-tert-butoxycarbonylamino-3-(2-fluoro-phenyl)-propionic acid are dissolved in 10 mL of methanol. The solution is cooled to 0°C and 450 μL
(6.14 mmol) of thionylchloride are added dropwise via a syringe. Then the mixture is allowed to warm to room temperature and stirred for 12 h. The solvent is removed under reduced pressure and the crude product is used in the next step without further purification.
Step 2
Figure imgf000096_0001
(ffl-2-Benzyloxycarbonylamino-3-(2-fluoro-phenyl)-propionic acid methyl ester 1.22 g (5.2 mmol) of crude (/?)-2-amino-3-(2-fiuoro-phenyl)-propionic acid methyl ester hydrochloride from step 1 are suspended in 10 mL of tetrahydrofuran. Then 1.81 mL (13.1 mmol) of triethylamine and 1.56 g (6.26 mmol) of Λ/-(benzyl- oxycarbonyloxy)succinimide are added, followed by enough water to completely dissolve the mixture. The resulting solution is stirred for 12 h at room temperature and the organic solvent is removed under reduced pressure. The residue is diluted with 10 mL of saturated sodium bicarbonate solution and extracted 3 times with ethyl acetate. The combined organic layers are washed with brine, dried with sodium sulphate and the solvent is evaporated. The crude product is used in step 3 without any further purification. Step 3
Figure imgf000097_0001
r(f?)-2-(2-Fluoro-phenvπ-1-hvdroxymethyl-ethyl1-carbamic acid benzyl ester
1.60 g (4.82 mmol) of (R)-2-benzyloxycarbonylamino-3-(2-fluoro-phenyl)-propionic acid methyl ester from step 2 is dissolved in 10 mL of tetrahydrofuran and the solution is cooled to 0°C Then 2.8 mL (5.78 mmol) of a 2 M solution of lithium borohydride in tetrahydrofuran are added dropwise and the mixture is allowed to warm to room temperature. After 2 h stirring at room temperature, methanol is added and the mixture is stirred for another 30 min. The solvent is evaporated and the residue is taken up in dichloromethane. The solution is washed with 1 M aqueous hydrochloric acid solution, saturated sodium bicarbonate solution, water and brine, dried with sodium sulphate and evaporated to dryness.
LCMS (la) rt 2,28 min, 260, 304 (M+H)+, 345 (M+CH3CN)+.
Step 4
Figure imgf000097_0002
Thioacetic acid S-f(/?)-2-benzyloxycarbonylamino-3-(2-fluoro-phenyl)-propyll ester 1.27 g (4.19 mmol) of [(R)-2-(2-fluoro-phenyl)-1-hydroxymethyl-ethyl]-carbamic acid benzyl ester from step 3 are dissolved in 20 mL of dichloromethane and the solution is cooled to 0°C. Then 1.28 mL (9.21 mmol) of triethylamine followed by 0.49 mL (6.28 mmol) methanesulfonylchlorid in 10 mL dichloromethane are added. The resulting mixture is stirred for 1 h at 0°C, washed with water, dried with sodium sulphate, and the solvent is removed under reduced pressure. The crude mesylate is dissolved in 10 mL Λ/,Λ/-dimethy] formamide and transfered into a flask, wrapped in aluminium foil, that had been charged with 0.89 g (2.73 mmol) cesium carbonate, 10 mL Λ/,Λ/-dimethyl formamide and 0.36 mL (5.03 mmol) thioacetic acid. The reaction mixture is stirred for 12 h at room temperature. Afterwards the solution is poured into 50 mL of brine and the mixture is extracted 3 times with ethyl acetate. The combined organic layers are washed with saturated sodium bicarbonate solution and brine, and is dried over sodium sulphate. Then the solvent is evaporated and the crude product is purified by column chromatography on silica gel with cyclohexane:ethyl acetate (80:20) as eluent. The product is further recrystalized from cyclohexane.
LCMS (la) rt 3.20 min, 302, 318, 363 (M+H)+, 384 (M-H+Na)+, 403 (M+CH3CN)+, 425 (M+CH3CN+Na)+.
Step 5
Figure imgf000098_0001
(R)-2-Benzyloxycarbonylamino-3-(2-fluoro-phenyl)-propane-1-sulphonate tetra-n-butyl ammonium salt
942 mg (2.61 mmol) of thioacetic acid S-[(R)-2-benzyloxycarbonylamino-3-(2-fluoro- phenyl)-propyl] ester from step 4 are dissolved in 80 mL of methanol and a solution of 4.00 g (6.50 mmolq) oxone in 80 mL of water is added. After stirring for 30 min at room temperature a solution of 4.00 mL of aqueous tetra-n-butyl ammonium hydroxide solution (40 %) in 20 mL of water is added and the resulting mixture is stirred for 12 h at room temperature. Then most of the methanol is evaporated and the residue is diluted with 80 mL of water and extracted 3 times with dichloromethane. The combined organic layers are dried with sodium sulphate and the solvent is evaporated. Step 6
Figure imgf000099_0001
r(ffl-1-Chlorosulphonylmethyl-2-(2-fluoro-phenyl)-ethyl1-carbamic acid benzyl ester 441 mg (0.72 mmol) of the tetra-n-butyl ammonium salt of (f?)-2-benzyloxycarbonyl- amino-3-(2-fluoro-phenyl)-propane-1-sulphonic acid from step 5 are dissolved in 4.5 mL of dichloromethane. To this mixture a solution of 144 mg (0.49 mmol) of triphosgene in 1 mL of dichloromethane is added and the reaction mixture is stirred for 2 h at room temperature. The solvent is removed under reduced pressure and the crude product is purified by column chromatography on silica gel with cyclo- hexane:ethyl acetate (70:30) as eluent.
Step 7
Figure imgf000099_0002
((f?)-2-(2-Fluoro-phenyl)-1-{[(6-trifluoromethyl-pyridin-3-ylmethvπ-sulphamoyl1-methyll- ethvP-carbamic acid benzyl ester
16.4 mg (0.09 mmol) of 3-aminomethyl-6-(trifluoromethyl)pyridine are dissolved in 500 μL dichloromethane and 20.3 mg (0.12 mmol) of 1-methoxy-1-(trimethylsiloxy)-2- methyl-1 -propane dissolved in 500 μL dichloromethane are added. This mixture is aged for 5 min at room temperature and added to a solution of 30.0 mg (0.08 mmol) of [(R)- 1-chlorosulphonylmethyl-2-(2-fluoro-phenyl)-ethyl]-carbamic acid benzyl ester from step 6 in 2 mL of dichloromethane. After stirring the solution for 48 h at room temperature the solvent is evaporated and the residue is purified by column chromatography on silica gel with cyclohexane:ethyl acetate (70:30) as eluent. LCMS (la) rt 4.33 min, 526 (M+H)+, 548 (M+Na)\ 589 (M+Na+CH3CN)+.
Step 8
Figure imgf000100_0001
(R)-2-Amino-3-(2-fluoro-phenyl)-propane-1 -sulphonic acid (6-trifluoromethyl-pyridin-3- ylmethvD-amide
15.5 mg (0.03 mmol) of ((R)-2-(2-fIuoro-phenyl)-1-{[(6-trifluoromethyl-pyridin-3- ylmethyl)-sulphamoyl]-methyl}-ethyl)-carbamic acid benzyl ester from step 7 are dissolved in 2 mL of methanol. Then 5.6 mg (0.09 mmol) of ammonium formate and 15 mg of 10 % palladium on charcoal is added and the reaction mixture is heated to reflux for 45 min. The mixture is filtered through celite and the filtrate is evaporated to dryness and purified by preparative HPLC.
1H-NMR (300 MHz, DMSO-d6) δ = 2.66-2.71 (m, 2H), 3.02-3.08 (m, 2H), 3.37-3.38 (m,
1 H), 4.19-4.30 (m, 2H), 7.14-7.32 (m, 5H), 7.90 (d, 1 H), 8.00 (d, 1 H), 8.70 (s, 1 H). '
LCMS (Va) r.6.82 min, 374, 392 (M+H)+, 415 (M+Na)+, 433 (M+CH3CN)+. Example 192 o H X OH
Figure imgf000101_0001
Steps 1- 6 are carried out according to example 191.
Step 7
Figure imgf000101_0002
{(f?)-2-(2-Fluoro-phenyl)-1-r(3-trifluoromethyl-benzylsulphamoyl)-methvn-ethyl) carbamic acid benzyl ester
16.3 mg (0.09 mmol) of 3-(trifluoromethyl)benzyl amine are dissolved in 500 μL dichloromethane and 20.3 mg (0.12 mmol) of 1-methoxy-1-(trimethylsiloxy)-2-methyl-1- propane dissolved in 500 μL dichloromethane are added. This mixture is aged for 5 min at room temperature and added to a solution of 30.0 mg (0.08 mmol) of [(R)-l- chlorosulfonylmethyl-2-(2-fluoro-phenyl)-ethyl]-carbamic acid benzyl ester in 2 mL of dichloromethane. After stirring the solution for 48 h at room temperature the solvent is evaporated and the residue is purified by column chromatography on silica gel with cyclohexane:ethyl acetate (70:30) as eluent.
LCMS (la) rt 4.65 min, 481 , 525 (M+H)+, 547 (M+Na)+, 588 (M+Na+CH3CN)+. Step 8
Figure imgf000102_0001
(f?)-2-Amino-3-(2-fluoro-phenyl)-propane-1 -sulphonic acid 3-trifluoromethyl-benzyl- amide
18.0 mg (0.03 mmol) of {(ft)-2-(2-fluoro-phenyl)-1-[(3-trifluoromethyl- benzylsulphamoyl)- methyl]-ethyl}-carbamic acid benzyl ester from step 7 are dissolved in 2 mL of methanol. Then 6.5 mg (0.10 mmol) of ammonium formate and 18 mg of
10 % palladium on charcoal is added and the reaction mixture is heated to reflux for 45 min. The mixture is filtered through celite and the filtrate is evaporated to dryness and purified by preparative HPLC.
1H-NMR (300 MHz, DMSO-d6) δ = 2.65-2.70 (m, 2H), 3.01-3.08 (m, 2H), 3.38-3.40 (m,
1 H), 4.12-4.24 (m, 2H), 7.14-7.32 (m, 5H), 7.58-7.68 (m, 4H).
LCMS (Va) rt 8.86 min, 373, 391 (M+H)+, 414 (M+Na)\ 432 (M+ CH3CN)+.
Example 193
Figure imgf000102_0002
Steps 1- 6 are carried out according to example 191. Step 7
Figure imgf000103_0001
((f?)-2-(2-Fluoro-phenyl)-1-{[methyl-(3-trifluoromethyl-benzyl)-sulphamovn-methyl)- ethvP-carbamic acid benzyl ester
30.0 mg (0.08 mmol) of [(ft)-1-chlorosulphonylmethyl-2-(2-fluoro-phenyl)-ethyl]- carbamic acid benzyl ester are dissolved in 2 mL of dichloromethane. Then 12.8 μL
(0.09 mmol) of triethylamine and 14.6 mg (0.08 mmol) of Λ/-methyl-Λ/-[3- trifluoromethyl)benzyl]amine are added and the resulting mixture is stirred for 12 h at room temperature.
LCMS (la) rt 4.89 min, 539 (M+H)+, 561 (M+Na)+, 602 (M+Na+CH3CN)+.
Step 8
Figure imgf000103_0002
(R -2-Amino-3-(2-fluoro-phenyl)-propane-1 -sulphonic acid methyl-(3-trifluoromethyl- benzvD-amide
17.7 mg (0.03 mmol) of ((R)-2-(2-Fluoro-phenyl)-1-{[methyl-(3-trifluoromethyI-benzyl)- sulphamoyl]-methyl}-ethyl)-carbamic acid benzyl ester from step 7 are dissolved in 2 mL of methanol. Then 6.2 mg (0.09 mmol) of ammonium formate and 18 mg of 10 % palladium on charcoal is added and the reaction mixture is heated to reflux for 45 min. The mixture is filtered through celite and the filtrate is evaporated to dryness and purified by preparative HPLC. 1H-NMR (300 MHz, DMSO-d6) δ = 2.68 (s, 3H), 2.68-2.93 (m, 2H), 3.45-3.46 (m, 1 H), 4.38 (s, 2H), 7.15-7.20 (m, 2H), 7.29-7.36 (m, 2H), 7.64-7.69 (m, 4H). LCMS (Va) rt 9.52 min, 405 (M+H)+, 446 (M+CH3CN)+.
ASSAYS
Inhibition of DPP-IV peptidase activity was monitored with a continuous fluorimetric assay. This assay is based on the cleavage of the substrate Gly-Pro-AMC (Bachem) by DPP-IV, releasing free AMC The assay is carried out in 96-well microtiterplates. In a total volume of 100 μL, compounds are preincubated with 50 pM DPP-IV employing a buffer containing 10mM Hepes, 150mM NaCl, 0.005% Tween 20 (pH 7.4). The reaction is started by the addition of 16 μM substrate and the fluorescence of liberated AMC is detected for 10 minutes at 25 °C with a fluorescence reader (BMG-Fluostar; BMG- , Technologies) using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. The final concentration of DMSO is 1 %. The inhibitory potential of the compounds were determined. DPP-IV activity assays were carried out with human and porcine DPP-IV (see below); both enzymes showed comparable activities.
Soluble human DPP-IV lacking the transmembrane anchor (Gly31-Pro766) was expressed in a recombinant YEAST-strain as Pre-Pro-alpha-mating fusion. The secreted product (rhuDPP-IV-Gly31-Pro766) was purified from fermentation broth (>90% purity).
In the table are listed the 1C50 values for inhibition of DPP-IV peptidase activity determined in assays as described above. The IC50 values were grouped in 3 classes: a < 100 nM; b >101 nM and < 1001 nM ; c >1001 nM < 2000 nM.
Figure imgf000105_0001
Figure imgf000105_0002
Figure imgf000105_0003
Figure imgf000105_0004
Figure imgf000106_0001
Figure imgf000106_0002
Figure imgf000106_0003

Claims

Claims
1. A compound of the formula (I)
Z-C(R1R2)-C(R3NH2)-C(R4R5)-X-N(R6R7) (I),
or a pharmaceutically acceptable salt thereof, wherein
Z is selected from the group consisting of phenyl; naphthyl; indenyl; C3.7 cycloalkyl; indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle, wherein Z is optionally substituted with one or more R8, wherein R8 is independently selected from the group consisting of halogen; CN; OH; NH2; oxo (=0), where the ring is at least partially saturated; R9; and R10;
R9 is selected from the group consisting of C|-6 alkyl; 0-C-ι-6 alkyl; and S-Cι-6 alkyl, wherein R9 is optionally interrupted by oxygen and wherein R9 is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R10 is selected from the group consisting of phenyl; heterocycle; and C3-7 cycloalkyl, wherein R10 is optionally substituted with one or more R11, wherein R11 is independently selected from the group consisting of halogen; CN; OH; NH2; oxo (=0), where the ring is at least partially saturated; Cι-6 alkyl; OO^e alkyl; and S-C|-6 alkyl;
R1, R4 are independently selected from the group consisting of H; F; OH; and R4a;
R2, R5 are independently selected from the group consisting of H; F; and R4b;
R4a is independently selected from the group consisting of C-i-e alkyl; and 0-C|-6 alkyl, wherein R4a is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R4b is Ci-6 alkyl, wherein R4b is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl; R3 is selected from the group consisting of H; and Cι-6 alkyl;
Optionally one or more pairs of R1, R2, R3, R4, R5 independently selected from the group consisting of R1/R2; R2/R3; R3/R4; and R4/R5 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more of R12, wherein R12 is independently selected from the group consisting of F; Cl; and OH;
X is selected from the group consisting of S(O); S(0)2; C(O); and C(RldR14);
R13, R14 are independently selected from the group consisting of H; F; d-6 alkyl; R15; and R16;
Optionally one or both pairs of R5, R13, R14 selected from the group consisting of R5/R13; and R13/R14 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more R17, wherein R17 is independently selected from the group consisting of F; Cl; and OH;
R15 is selected from the group consisting of phenyl; naphthyl; and indenyl, wherein R15 is optionally substituted with one or more R18, wherein R18 is independently selected from the group consisting of R19; R20; halogen; CN; COOH; OH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; O-C^ alkyl; S-d-6 alkyl; COO-C^ alkyl; OC(0)-C1-6 alkyl; C(0)N(R21)-d-6 alkyl; S(0)2N(R21)-Cι-6 alkyl; S(0)N(R21)-d-6 alkyl; S(0)2-d-6 alkyl; S(0)-C1-6 alkyl; N(R21)S(0)2-C1-6 alkyl; and N(R21)S(0)-d-6 alkyl, wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R16 is selected from the group consisting of heterocycle; heterobicycle; C3-7 cycloalkyl; indanyl; tertralinyl; and decalinyl, wherein R16 is optionally substituted with one or more R22, wherein R22 is independently selected from the group consisting of R19; R20; halogen; CN; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-6 alkyl; 0-d-6 alkyl; S-d-6 alkyl; N(R23)-d-6 alkyl; COO-d-6 alkyl; OC(0)-d.6 alkyl; C(0)N(R23)- d-6 alkyl; N(R23)-C(0)- d-6 alkyl; S(0)2N(R 3)-d-6 alkyl; S(0)N(R23)-C1-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R23)S(0)2-C1-6 alkyl; and N(R23)S(0)-C1-6 alkyl, wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R19 is selected from the group consisting of phenyl; and naphthyl, wherein R19 is optionally substituted with one or more R24, wherein R24 is independently selected from the group consisting of halogen; CN; COOH; OH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; 0-d-6 alkyl; S-d-6 alkyl; COO-C^ alkyl; OC(0)-Cι-6 alkyl; C(0)N(R25)-d-6 alkyl; S(0)2N(R25)-d-6 alkyl; S(0)N(R25)-d-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R25)S(0)2-d-6 alkyl; and N(R25)S(0) -d-6 alkyl, wherein each C1-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R20 is selected from the group consisting of heterocycle; heterobicycle; and C3_7 cycloalkyl; wherein R20 is optionally substituted with one or more R26, wherein R26 is independently selected from the group consisting of halogen; CN; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-e alkyl; O-C^ alkyl; S-C1.6 alkyl; N(R27)-C1-6 alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R27)- d-6 alkyl; N(R27)-C(0)-C1-6 alkyl; S(0)2N(R27)-d-6 alkyl; S(0)N(R27)-d-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R27)S(0)2-d-6 alkyl; and N(R27)S(0)-Cι-6 alkyl wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R2\ R23, R25, R27 are independently selected from the group consisting of H; and Cι-6alkyl, which is optionally substituted with one or more of R28, wherein R28 is independently selected from the group consisting of F; Cl and OH;
R6, R7 are independently selected from the group consisting of H; (C(R29R30))m-X1-Z1; and (C(R31R32))n-X2-X3-Z2, provided that R6, R7 are selected so that not both of R6, R7 are independently selected from the group consisting of H; CH3; CH2CH3; CH2CH2CH3; and CH(CH3)2;
Optionally R6, R7 are independently d-4 alkyl, which is substituted with one or more R29a, wherein R29a is independently selected from the group consisting of R29b; and Z1, provided that R6, R7 are selected so that not both of R6, R7 are independently selected from the group consisting of CH3; CH2CH3; CH2CH2CH3; and CH(CH3)2; R29, R29b, R30, R31, R32 are independently selected from the group consisting of H halogen; CN; OH; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-6 alkyl; 0-Cι-6 alkyl N(R32a)-d-6 alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R32a)- C,-6 alkyl N(R32a)-C(0)-d-6 alkyl; S(0)2N(R32a)-d-6 alkyl; S(0)N(R32a)-d-6 alkyl; S(0)2-d-6 alkyl S(0)-C1-6 alkyl; N(R32a)S(0)2-d-6 alkyl; and N(R32a)S(0)-d-6 alkyl wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R32a is selected from the group consisting of H; and Cι-6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Optionally one or more pairs of R29, R30, R31, R32 independently selected from the group consisting of R29/R30; and R31/R32 form a C3-7 cycloalkyl ring, which is optionally substituted with one or more R32 , wherein R32b is independently selected from the •group consisting of F; Cl; and OH;
m is 0, 1 , 2, 3 or 4;
n is 2, 3 or 4;
X1 is independently selected from the group consisting of a covalent bond ■d-e alkyl-; -d-6 alkyl-O-; -d-6 alkyl-N(R33)-; -C(O)-; -C(0)-d-6 alkyl- -C(0)-d-6 alkyl-O-; -C(0)-d-6 alkyl-N(R33)-; -C(0)0-; -C(0)0-d-6 alkyl- -C(0)0-d-6 alkyl-O-; -C(0)0-d-6 alkyl-N(R33)-; -C(0)N(R33)-; -C(0)N(R33)-d-6 alkyl- -C(0)N(R33)-d-6 alkyl-O-; -C(0)N(R33)-d-6 alkyl-N(R34)-; -S(0)2-; -S(O)- ^(O^-C^e alkyl-; -S(0)-d-6 alkyl-; -S(0)2-Cι-6 alkyl-O-; -S^-C^ alkyl-O-
Figure imgf000110_0001
alkyl-N(R33)-; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
X2 is selected from the group consisting of -0-; -S-; -S(O)-; S(0)2-; and -N(R35)-; X3 is selected from the group consisting of a covalent bond; -Cι-6 alkyl-; -Cι-6 alkyl-O- -d-6 alkyl-N(R36)-; -C(O)-; -C(0)-C1-6 alkyl-; -C(0)-d-6 alkyl-O-; -C(0)-C1-6 alkyl-N(R36)- -C(0)0-; -C(0)0-d-6 alkyl-; -C(0)0-C1-6 alkyl-O-; -C(0)0-d-6 alkyl-N(R36)- -C(0)N(R36)-; -C(0)N(R36)-C1-6 alkyl-; -C(0)N(R36)-d-6 alkyl-O-; and -C(0)N(R36)-d-6 alkyl-N(R37)-; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Optionally X2-X3 are independently selected from the group consisting of -N(R35)-S(0)2 -N(R35)-S(0)-; -N(R35)-S(0)2-d.6 alkyl-; -N(R35)-S(0)-d-6 alkyl-
-N(R35)-S(0)2-d-6 alkyl-O-; -N(R35)-S(0)-d-6 alkyl-O-; -N(R35)-S(0)2-d-6 alkyl-N(R36)- and -N(R35)-S(0)-C1-6 alkyl-N(R36)-; wherein each d-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
R33, R34, R35, R36, R37 are independently selected from the group consisting of H; and d-6 alkyl, which is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
Z1, Z2 are independently selected from the group consisting of Z3; and -C(R37a)Z3aZ3b;
R37a is selected from the group consisting of H; and Cι-6 alkyl, which is optionally substituted with one or more F;
Z3, Z3a, Z3b are independently selected from the group consisting of H; T1; T2; Ci-6 alkyl; d-6 alkyl-T1; and C-ι-6 alkyl-T2; wherein each d-6 alkyl is optionally substituted with one or more R37b, wherein R37b is independently selected from the group consisting of halogen; CN; OH; NH2; COOH; C(0)NH2; S(0)2NH2; S(0)NH2; d-6 alkyl; 0-d.6 alkyl; N(R37c)-d-6 alkyl; COO-d-6 alkyl; OC(0)-d-6 alkyl; C(0)N(R37c)- d_6 alkyl; N(R37c)-C(0)-d-6 alkyl; S(0)2N(R37c)-C1-6 alkyl; S(0)N(R37c)-d-6 alkyl; S(0)2-d-6 alkyl; S(0)-d-6 alkyl; N(R37c)S(0)2-d-6 alkyl; and N(R37c)S(0)-d-6 alkyl; wherein each C1-6 alkyl is optionally substituted with one or more halogen independently selected from the group consisting of F; and Cl;
T1 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T1 is optionally substituted with one or more R38; wherein R38 is independently selected from the group consisting of halogen; CN; R39; COOH; OH; C(0)NH2; ill
S(0)2NH2; S(0)NH2; COOT3; OT3; ST3; C(O)N(R40)T3; S(O)2N(R40)T3; S(O)N(R40)T and T3;
T2 is selected from the group consisting of C3-7 cycloalkyl; indanyl; tetralinyl; decalinyl; heterocycle; and heterobicycle; wherein T2 is optionally substituted with one or more R41, wherein R41 is independently selected from the group consisting of halogen; CN R42; OH; oxo (=0), where the ring is at least partially saturated; NH2; COOH; C(0)NH2 S(0)2NH2; S(0)NH2; COOT3; OT3; C(0)N(R43)T3; S(0)2N(R43)T3; S(0)N(R43)T3 N(R43)T3; and T3;
R39 is selected from the group consisting of d-6 alkyl; 0-d-6 alkyl; S-C^e alkyl; COO-d-6 alkyl; OC(O)- d-6 alkyl; C(0)N(R44)-d.6 alkyl; S(0)2N(R44)-d-6 alkyl; S(0)N(R44)-d-6 alkyl; S(0)-d-6 alkyl; S(0)2-d-6 alkyl; N(R44)S(0)2-d-6 alkyl; and N(R44)S(0) -Ci-6 alkyl; wherein each d-6 alkyl is optionally substituted with one more R45, wherein R45 is independently selected from the group consisting of F; COOR46; C(0)N(R46R47); S(0)2N(R46R47); OR46; N(R46R47); T3; O-T3; and N(R46)-T3;
R42 is selected from the group consisting of d-6 alkyl; 0-d-6 alkyl; S-C-ι-6 alkyl; N(R48)-d-6 alkyl; COO-C^ alkyl; OC(O)- d.6 alkyl; C(0)N(R48)- d-6 alkyl; N(R48)-C(O)- d-6 alkyl; S(0)2N(R48)-d-6 alkyl; S(O) N(R48)-d-6 alkyl; S(0)-d-6 alkyl; S(0)2-d-6 alkyl; -N(R48)S(0)2-C1-6 alkyl; and -N(R48)S(0)-C1-6 alkyl; wherein each d-6 alkyl is optionally substituted with one or more R45, wherein R45 is independently selected from the group consisting of F; COOR49; C(0)N(R49R5°); S(0)2N(R49R5°); S(0)N(R49R50); OR49; N(R49R50); T3; O-T3; and N(R49)-T3;
R40, R43, R44, R46, R47, R48, R49, R50 are independently selected from the group consisting of H; and d-6 alkyl;
T3 is selected from the group consisting of T4; and T5;
T4 is selected from the group consisting of phenyl; naphthyl; and indenyl; wherein T4 is optionally substituted with one or more R51, wherein R51 is independently selected from the group consisting of halogen; CN; COOR52; OR52; C(0)N(R52R53); S(0)2N(R52R53); Ci-6 alkyl; 0-d-6 alkyl; S-d-6 alkyl; COO-C^ alkyl; 00(0)^-6 alkyl; C(0)N(R52)- d-e alkyl; S(0)2N(R52)-C1-6 alkyl; S(0)N(R52)-d-6 alkyl; S(0)2-d-6 alkyl; S(O) -Ci-6 alkyl; N(R52)S(0)2-C1-6 alkyl; and N(R52)S(0)-C1-6 alkyl; wherein each d-6 alkyl is optionally substituted with one more halogen selected from the group consisting of F; and Cl;
T5 is selected from the group consisting of heterocycle; heterobicycle; C3-7 cycloalkyl; indanyl; tetralinyl; and decalinyl; wherein T5 is optionally substituted with one or more R54, wherein R54 is independently selected from the group consisting of halogen; CN; OR55; oxo (=0), where the ring is at least partially saturated; N(R55R56) COOR55; C(0)N(R55R56); S(0)2N(R55R56); S(0)N(R55R56); d-6 alkyl; O-C^ alkyl S-Cϊ-e alkyl; N(R55)-C1-6 alkyl; COO-C-, alkyl; OC(O)- d-6 alkyl; C(0)N(R55)- d-6 alkyl N(R55)-C(0)-d-6 alkyl; S(0)2N(R55)-d-6 alkyl; S(0)N(R55)-C1-6 alkyl; S(0)2-d-6 alkyl S(0)-d-6 alkyl; N(R55)S(0)2-d-6 alkyl; and N(R55)S(0)-C1-6 alkyl; wherein each Ci-6 alkyl is optionally substituted with one more halogen selected from the group consisting of F; and Cl;
,R52, R53, R55, R56, are independently selected from the group consisting of H; and Ci-6 alkyl. '■
2. A compound according to claim 1 of formula (la)
Figure imgf000113_0001
or a pharmaceutically acceptable salt thereof, wherein Z, R1-R7 and X have the meaning as indicated in claim 1.
3. A compound according to claim 1 or 2, wherein Z is phenyl or heterocycle.
4. A compound according to any one of the preceding claims, wherein Z is optionally substituted with 1 or 2 R8, which are the same or different.
5. A compound according to any one of the preceding claims, wherein R8 is selected from the group consisting of Cl; F; CN; CH3; and OCH3.
6. A compound according to any one of the preceding claims, wherein Z is 2-Fluoro-phenyl.
7. A compound according to any one of the preceding claims, wherein R1, R4 are independently selected from the group consisting of H; F; OH; CH3; and OCH3.
8. A compound according to any one of the preceding claims, wherein R2, R5 are independently selected from the group consisting of H; F; and CH3.
9. A compound according to any one of the preceding claims, wherein R1, R2, R4, R5 are H.
10. A compound according to any one of the preceding claims, wherein R3 is H.
11. A compound according to any one of the preceding claims, wherein X is C(O) or S(0)2.
12. A compound according to any one of the preceding claims, wherein R6 is selected from the group consisting of H; and CH3.
13. A compound according to any one of the preceding claims, wherein X1 is a covalent bond.
14. A compound according to any one of the preceding claims, wherein m is 0, 1 , 2 or 3.
15. A compound according to any one of the preceding claims, wherein R7 is Z1.
16. A compound according to any one of the preceding claims, wherein R7 is d-4 alkyl, substituted with 1-4 R29a, which are the same or different.
17. A compound according to claim 16, wherein R7 is selected from the group consisting of CH(R29a)2; CHR29a-CH2R29a; CH2-CH(R29a)2; CH2-CHR29a-CH2R29a; and CH2-CH2-CH(R29a)2.
18. A compound according to any one of the preceding claims, wherein R29a is selected from the group consisting of R29 ; and Z1; and wherein R29 is selected from the group consisting of H; F; Cl; NH2; NHCH3; N(CH3)2; CH3; and C2H5.
19. A compound according to any one of the preceding claims, wherein R29a is selected from the group consisting of R29b; and Z1; and wherein Z is selected from the group consisting of T1; and T2.
20. A compound according to any one of the preceding claims, wherein T1 is phenyl; and wherein T1 is optionally substituted with 1-3 R38, which are the same or different.
21. A compound according to any one of the preceding claims, wherein R38 is independently selected from the group consisting of F; Cl; CN; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; 0-CH3; 0-C2H5; S-CH3; S02NH2; T3; and O-T3.
22. A compound according to any one of the preceding claims, wherein T2 is selected from the group consisting of
Figure imgf000115_0001
Figure imgf000116_0001
and wherein T2 is optionally substituted with 1-2 R41, which are the same or different.
23. A compound according to any one of the preceding claims, wherein R41 is selected from the group consisting of OH; CH3; and T3;
24. A compound according to any one of the preceding claims, wherein T3 is T4.
25. A compound according to any one of the preceding claims', wherein T4 is phenyl, ' wherein T4 is optionally substituted with 1-3 R5 , which are the same or different.
26. A compound according to any one of the preceding claims, wherein R51 is independently selected from the group consisting of F; Cl; CH3; C2H5; CH2CH2CH3; CH(CH3)2; CF3; 0-CH3; 0-C2H5; S-CH3; and S02NH2.
27. A compound according to any one of the preceding claims, wherein T is T .
28. A compound according to any one of the preceding claims, wherein T5 is heterocycle, wherein T5 is optionally substituted with 1-2 R54, which are the same or different.
29. A compound according to any one of the preceding claims, wherein R54 is selected from the group consisting of OH; and CH3.
30. A compound according to claim 1 selected from the group consisting of
Figure imgf000117_0001
Figure imgf000117_0002
Figure imgf000118_0001
Figure imgf000119_0001
Figure imgf000120_0001
Figure imgf000121_0001
Figure imgf000122_0001
Figure imgf000122_0002
31. A prodrug compound of a compound according to any one of the claims 1 to 30.
32. A pharmaceutical composition comprising a compound or a pharmaceutically acceptable salt thereof according to any one of the claims 1 to 31 together with a pharmaceutically acceptable carrier.
33. A pharmaceutical composition according to claim 32, comprising one or more additional compounds or pharmaceutically acceptable salts thereof selected from the group consisting of another compound according to any one of the claims 1 to 27; another DPP-IV inhibitor; insulin sensitizers; PPAR agonists; biguanides; protein tyrosinephosphatase-IB (PTP-1 B) inhibitors; insulin and insulin mimetics; sulfonylureas and other insulin secretagogues; a-glucosidase inhibitors; glucagon receptor antagonists; GLP-1 , GLP-1 mimetics, and GLP-1 receptor agonists; GIP, GIP mimetics, and GIP receptor agonists; PACAP, PACAP mimetics, and PACAP receptor 3 agonists; cholesterol lowering agents; HMG-CoA reductase inhibitors; sequestrants; nicotinyl alcohol; nicotinic acid or a salt thereof; PPARa agonists; PPARoly dual agonists; inhibitors of cholesterol absorption; acyl CoA : cholesterol acyltransferase inhibitors; anti-oxidants; PPARo agonists; antiobesity compounds; an ileal bile acid transporter inhibitor; and anti-inflammatory agents.
34. A compound or a pharmaceutically acceptable salt thereof of any one of the claims 1 to 31 for use as a medicament.
35. Use of a compound or a pharmaceutically acceptable salt thereof of any of the claims 1 to 31 for the manufacture of a medicament for the treatment or prophylaxis of non-insulin dependent (Type II) diabetes mellitus; hyperglycemia; obesity; insulin resistance; lipid disorders; dyslipidemia; hyperlipidemia; hypertriglyceridemia; hypercholestrerolemia; low HDL; high LDL; atherosclerosis; growth hormone deficiency; diseases related to the immune response; HIV infection; neutropenia; neuronal disorders; tumor metastasis; benign prostatic hypertrophy; gingivitis; hypertension; osteoporosis; diseases related to sperm motility; low glucose tolerance; insulin resistance; ist sequelae; vascular restenosis; irritable bowel syndrome; inflammatory bowel disease; including Crohn's disease and ulcerative colitis; other inflammatory conditions; pancreatitis; abdominal obesity; neurodegenerative disease; anxiety; depression; retinopathy; nephropathy; neuropathy; Syndrome X; ovarian hyperandrogenism (polycystic ovarian syndrome; Type n diabetes; or growth hormone deficiency.
36. Use of a compound according to any one of the claims 1 to 31 as DPP-IV inhibitor.
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WO2012170702A1 (en) 2011-06-08 2012-12-13 Arena Pharmaceuticals, Inc. Modulators of the gpr119 receptor and the treatment of disorders related thereto
US8664232B2 (en) 2002-08-21 2014-03-04 Boehringer Ingelheim Pharma Gmbh & Co. Kg 8-[3-amino-piperidin-1-yl]-xanthines, the preparation thereof and their use as pharmaceutical compositions
US8673927B2 (en) 2006-05-04 2014-03-18 Boehringer Ingelheim International Gmbh Uses of DPP-IV inhibitors
US8697868B2 (en) 2004-02-18 2014-04-15 Boehringer Ingelheim International Gmbh 8-[3-amino-piperidin-1-yl]-xanthines, their preparation and their use as pharmaceutical compositions
US8846695B2 (en) 2009-01-07 2014-09-30 Boehringer Ingelheim International Gmbh Treatment for diabetes in patients with inadequate glycemic control despite metformin therapy comprising a DPP-IV inhibitor
US8853156B2 (en) 2008-08-06 2014-10-07 Boehringer Ingelheim International Gmbh Treatment for diabetes in patients inappropriate for metformin therapy
US8865729B2 (en) 2008-12-23 2014-10-21 Boehringer Ingelheim International Gmbh Salt forms of a xanthine compound
US8883805B2 (en) 2004-11-05 2014-11-11 Boehringer Ingelheim International Gmbh Process for the preparation of chiral 8-(3-aminopiperidin-1-yl)-xanthines
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US9034883B2 (en) 2010-11-15 2015-05-19 Boehringer Ingelheim International Gmbh Vasoprotective and cardioprotective antidiabetic therapy
US9149478B2 (en) 2010-06-24 2015-10-06 Boehringer Ingelheim International Gmbh Diabetes therapy
US9155705B2 (en) 2008-04-03 2015-10-13 Boehringer Ingelheim International Gmbh DPP-IV inhibitor combined with a further antidiabetic agent, tablets comprising such formulations, their use and process for their preparation
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US9266888B2 (en) 2006-05-04 2016-02-23 Boehringer Ingelheim International Gmbh Polymorphs
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US11911388B2 (en) 2008-10-16 2024-02-27 Boehringer Ingelheim International Gmbh Treatment for diabetes in patients with insufficient glycemic control despite therapy with an oral or non-oral antidiabetic drug

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JO3301B1 (en) 2013-04-26 2018-09-16 Albireo Ab Crystal modifications of elobixibat
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CN116157389A (en) 2020-08-03 2023-05-23 阿尔比里奥公司 Benzothiazepine compounds and their use as bile acid modulators
EP4243831A1 (en) 2020-11-12 2023-09-20 Albireo AB Odevixibat for treating progressive familial intrahepatic cholestasis (pfic)
BR112023010799A2 (en) 2020-12-04 2023-10-03 Albireo Ab BENZOTIA(DI)AZEPINE COMPOUNDS AND THEIR USES AS BILLARY ACID MODULATORS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000181A2 (en) * 2001-06-20 2003-01-03 Merck & Co., Inc. Dipeptidyl peptidase inhibitors for the treatment of diabetes
WO2003000180A2 (en) * 2001-06-20 2003-01-03 Merck & Co., Inc. Dipeptidyl peptidase inhibitors for the treatment of diabetes
WO2004085378A1 (en) * 2003-03-19 2004-10-07 Merck & Co. Inc. Process for the preparation of chiral beta amino acid derivatives by asymmetric hydrogenation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455271A (en) * 1992-06-18 1995-10-03 The Scripps Research Institute Tight-binding inhibitors of leukotriene A4 hydrolase
DE19616486C5 (en) * 1996-04-25 2016-06-30 Royalty Pharma Collection Trust Method for lowering the blood glucose level in mammals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000181A2 (en) * 2001-06-20 2003-01-03 Merck & Co., Inc. Dipeptidyl peptidase inhibitors for the treatment of diabetes
WO2003000180A2 (en) * 2001-06-20 2003-01-03 Merck & Co., Inc. Dipeptidyl peptidase inhibitors for the treatment of diabetes
WO2004085378A1 (en) * 2003-03-19 2004-10-07 Merck & Co. Inc. Process for the preparation of chiral beta amino acid derivatives by asymmetric hydrogenation

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CANTEL, SONIA ET AL: "Synthesis of chiral .alpha.-amino-diazoketones on solid support: an access to .beta.-homologated amino acid derivatives", SYNLETT , (15), 2791-2793 CODEN: SYNLES; ISSN: 0936-5214, 8 November 2004 (2004-11-08), XP001206415 *
GUDE, MARKUS ET AL: "A new method for the solution and solid phase synthesis of chiral .beta.-sulfonopeptides under mild conditions", TETRAHEDRON LETTERS , 37(47), 8589-8592 CODEN: TELEAY; ISSN: 0040-4039, 1996, XP004068724 *
MARUGG J L ET AL: "Selective catch and release of a synthetically useful phosphine ligand", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 44, no. 40, 29 September 2003 (2003-09-29), pages 7537 - 7540, XP004453464, ISSN: 0040-4039 *
MOREE, WILNA J. ET AL: "Synthesis of Peptidosulfinamides and Peptidosulfonamides: Peptidomimetics Containing the Sulfinamide or Sulfonamide Transition-State Isostere", JOURNAL OF ORGANIC CHEMISTRY , 60(16), 5157-69 CODEN: JOCEAH; ISSN: 0022-3263, 1995, XP002953415 *
RAJU, B. ET AL: "Conformationally restricted analogs of deoxynegamycin", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS , 14(12), 3103-3107 CODEN: BMCLE8; ISSN: 0960-894X, 2004, XP004841352 *
S.R.STARCK ET AL: "The puromycin route to assess stereo- and regiochemical constraints on peptide bond formation in eukaryotic ribosomes", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 125, no. 27, 6 November 2003 (2003-11-06), pages 8090 - 8091, XP002284840 *
SAVIANO, GABRIELLA ET AL: "Influence of conformational flexibility on biological activity in cyclic astin analogues", BIOPOLYMERS , 76(6), 477-484 CODEN: BIPMAA; ISSN: 0006-3525, 15 September 2004 (2004-09-15), XP002328124 *

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US9526728B2 (en) 2014-02-28 2016-12-27 Boehringer Ingelheim International Gmbh Medical use of a DPP-4 inhibitor
WO2017044742A1 (en) * 2015-09-09 2017-03-16 The Regents Of The University Of California Novel cyp34a-specific inhibitors and methods of using same
US10155000B2 (en) 2016-06-10 2018-12-18 Boehringer Ingelheim International Gmbh Medical use of pharmaceutical combination or composition
EP3595777A4 (en) * 2017-03-14 2021-01-20 Dana-Farber Cancer Institute, Inc. Small molecule sensitization of bax activation for induction of cell death
EP3461819A1 (en) 2017-09-29 2019-04-03 Probiodrug AG Inhibitors of glutaminyl cyclase

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RU2006131597A (en) 2008-04-10
KR20070007816A (en) 2007-01-16
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