WO2005090990A3 - Cytotoxicity assay - Google Patents

Cytotoxicity assay Download PDF

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Publication number
WO2005090990A3
WO2005090990A3 PCT/US2005/001993 US2005001993W WO2005090990A3 WO 2005090990 A3 WO2005090990 A3 WO 2005090990A3 US 2005001993 W US2005001993 W US 2005001993W WO 2005090990 A3 WO2005090990 A3 WO 2005090990A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytotoxicity assay
cytotoxicity
methods
flow cytometry
relates
Prior art date
Application number
PCT/US2005/001993
Other languages
French (fr)
Other versions
WO2005090990A2 (en
Inventor
Pamela Dunn
Liwei He
Laszlo Radvanyi
Danielle Salha
Original Assignee
Sanofi Pasteur Inc
Pamela Dunn
Liwei He
Laszlo Radvanyi
Danielle Salha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Pasteur Inc, Pamela Dunn, Liwei He, Laszlo Radvanyi, Danielle Salha filed Critical Sanofi Pasteur Inc
Priority to MXPA06008336A priority Critical patent/MXPA06008336A/en
Priority to EP05736477A priority patent/EP1706744A2/en
Priority to JP2006551314A priority patent/JP2007522447A/en
Priority to CA002559394A priority patent/CA2559394A1/en
Priority to AU2005224597A priority patent/AU2005224597A1/en
Priority to BRPI0506488-0A priority patent/BRPI0506488A/en
Publication of WO2005090990A2 publication Critical patent/WO2005090990A2/en
Priority to IL176938A priority patent/IL176938A0/en
Publication of WO2005090990A3 publication Critical patent/WO2005090990A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to methods for detecting CTL activity using flow cytometry to detect cleavage of cytotoxicity-related enzymes.
PCT/US2005/001993 2004-01-23 2005-01-21 Cytotoxicity assay WO2005090990A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
MXPA06008336A MXPA06008336A (en) 2004-01-23 2005-01-21 Cytotoxicity assay.
EP05736477A EP1706744A2 (en) 2004-01-23 2005-01-21 Cytotoxicity assay
JP2006551314A JP2007522447A (en) 2004-01-23 2005-01-21 Cytotoxicity assay
CA002559394A CA2559394A1 (en) 2004-01-23 2005-01-21 Cytotoxicity assay
AU2005224597A AU2005224597A1 (en) 2004-01-23 2005-01-21 Cytotoxicity assay
BRPI0506488-0A BRPI0506488A (en) 2004-01-23 2005-01-21 cytotoxicity assay
IL176938A IL176938A0 (en) 2004-01-23 2006-07-18 Cytotoxicity assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53899304P 2004-01-23 2004-01-23
US60/538,993 2004-01-23

Publications (2)

Publication Number Publication Date
WO2005090990A2 WO2005090990A2 (en) 2005-09-29
WO2005090990A3 true WO2005090990A3 (en) 2007-01-11

Family

ID=34966212

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/001993 WO2005090990A2 (en) 2004-01-23 2005-01-21 Cytotoxicity assay

Country Status (11)

Country Link
EP (1) EP1706744A2 (en)
JP (1) JP2007522447A (en)
KR (1) KR20070001930A (en)
CN (1) CN101065669A (en)
AU (1) AU2005224597A1 (en)
BR (1) BRPI0506488A (en)
CA (1) CA2559394A1 (en)
IL (1) IL176938A0 (en)
MX (1) MXPA06008336A (en)
WO (1) WO2005090990A2 (en)
ZA (1) ZA200605715B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013126720A2 (en) * 2012-02-22 2013-08-29 The Trustees Of The University Of Pennsylvania Compositions and methods for the assessment of target cancer cell resistance to car engineered t cell killing
US10568947B2 (en) 2014-07-21 2020-02-25 Novartis Ag Treatment of cancer using a CLL-1 chimeric antigen receptor
SG11201700476VA (en) 2014-07-21 2017-02-27 Novartis Ag Treatment of cancer using humanized anti-bcma chimeric antigen receptor
JP6604975B2 (en) * 2015-02-09 2019-11-13 株式会社ライフアートビレッジ Peptide screening method for immune cell therapy
CN105547971B (en) * 2016-01-08 2018-05-01 首都医科大学附属北京友谊医院 The flow cytometry assays of cytotoxic T cell degranulation
CN105445171B (en) * 2016-01-08 2018-03-02 首都医科大学附属北京友谊医院 The flow cytometry assays of NK degranulation
US11608382B2 (en) 2018-06-13 2023-03-21 Novartis Ag BCMA chimeric antigen receptors and uses thereof
CN118401673A (en) * 2021-12-06 2024-07-26 上海药明生物技术有限公司 3D bioassays for measuring antibody-dependent cell-mediated cytotoxicity

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030211548A1 (en) * 2002-01-29 2003-11-13 Oncolmmunin, Inc. Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
WO2005049800A2 (en) * 2003-11-14 2005-06-02 Fred Hutchinson Cancer Research Center Methods for screening for agents capable of modulating t lymphocyte function in response to a herpes simplex virus-infected cell

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030211548A1 (en) * 2002-01-29 2003-11-13 Oncolmmunin, Inc. Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
WO2005049800A2 (en) * 2003-11-14 2005-06-02 Fred Hutchinson Cancer Research Center Methods for screening for agents capable of modulating t lymphocyte function in response to a herpes simplex virus-infected cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JEROME K R ET AL: "Measurement of CTL-induced cytotoxicity: the caspase 3 assay.", APOPTOSIS : AN INTERNATIONAL JOURNAL ON PROGRAMMED CELL DEATH. DEC 2003, vol. 8, no. 6, December 2003 (2003-12-01), pages 563 - 571, XP002402951, ISSN: 1360-8185 *

Also Published As

Publication number Publication date
CA2559394A1 (en) 2005-09-29
AU2005224597A1 (en) 2005-09-29
KR20070001930A (en) 2007-01-04
IL176938A0 (en) 2006-12-10
WO2005090990A2 (en) 2005-09-29
ZA200605715B (en) 2007-04-25
MXPA06008336A (en) 2007-04-17
BRPI0506488A (en) 2007-02-13
JP2007522447A (en) 2007-08-09
CN101065669A (en) 2007-10-31
EP1706744A2 (en) 2006-10-04

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