WO2005090597A3 - Calibration standard for determining base proportions of degenerated bases in dna - Google Patents

Calibration standard for determining base proportions of degenerated bases in dna Download PDF

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Publication number
WO2005090597A3
WO2005090597A3 PCT/EP2005/003211 EP2005003211W WO2005090597A3 WO 2005090597 A3 WO2005090597 A3 WO 2005090597A3 EP 2005003211 W EP2005003211 W EP 2005003211W WO 2005090597 A3 WO2005090597 A3 WO 2005090597A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
calibration standard
determining base
degenerated bases
base proportions
Prior art date
Application number
PCT/EP2005/003211
Other languages
French (fr)
Other versions
WO2005090597A2 (en
Inventor
Joern Lewin
Original Assignee
Epigenomics Ag
Joern Lewin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Joern Lewin filed Critical Epigenomics Ag
Priority to EP05751732A priority Critical patent/EP1725685A2/en
Publication of WO2005090597A2 publication Critical patent/WO2005090597A2/en
Publication of WO2005090597A3 publication Critical patent/WO2005090597A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a calibration standard and a method for determining base proportions of degenerated bases in DNA and a method for producing this standard.
PCT/EP2005/003211 2004-03-19 2005-03-21 Calibration standard for determining base proportions of degenerated bases in dna WO2005090597A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05751732A EP1725685A2 (en) 2004-03-19 2005-03-21 Calibration standard for determining base proportions of degenerated bases in dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55480804P 2004-03-19 2004-03-19
US60/554,808 2004-03-19

Publications (2)

Publication Number Publication Date
WO2005090597A2 WO2005090597A2 (en) 2005-09-29
WO2005090597A3 true WO2005090597A3 (en) 2005-10-27

Family

ID=34970355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/003211 WO2005090597A2 (en) 2004-03-19 2005-03-21 Calibration standard for determining base proportions of degenerated bases in dna

Country Status (2)

Country Link
EP (1) EP1725685A2 (en)
WO (1) WO2005090597A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE535621T1 (en) 2006-10-18 2011-12-15 Epigenomics Ag MOLECULE FOR PROVIDING A STANDARD FOR THE QUANTITATIVE ANALYSIS OF THE METHYLATION STATUS OF A NUCLEIC ACID

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082600A1 (en) * 2001-03-09 2003-05-01 Alexander Olek Highly sensitive method for the detection of cytosine methylation patters
WO2004051224A2 (en) * 2002-12-03 2004-06-17 Illumina, Inc. Multiplexed methylation detection methods
WO2005075671A1 (en) * 2004-02-05 2005-08-18 Epigenomics Ag Method for the calibration and verification of methylation analysis methods with the aid of non-methylated dna

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082600A1 (en) * 2001-03-09 2003-05-01 Alexander Olek Highly sensitive method for the detection of cytosine methylation patters
WO2004051224A2 (en) * 2002-12-03 2004-06-17 Illumina, Inc. Multiplexed methylation detection methods
WO2005075671A1 (en) * 2004-02-05 2005-08-18 Epigenomics Ag Method for the calibration and verification of methylation analysis methods with the aid of non-methylated dna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JU J ET AL: "FLUORESCENCE ENERGY TRANSFER DYE-LABELED PRIMERS FOR DNA SEQUENCINGAND ANALYSIS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 92, May 1995 (1995-05-01), pages 4347 - 4351, XP002052420, ISSN: 0027-8424 *
QIU P J ET AL: "Quantification of single nucleotide polymorphisms by automated DNA sequencing", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 309, no. 2, 19 September 2003 (2003-09-19), pages 331 - 338, XP004451517, ISSN: 0006-291X *

Also Published As

Publication number Publication date
EP1725685A2 (en) 2006-11-29
WO2005090597A2 (en) 2005-09-29

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