WO2005079531A3 - Method and system for eluting cells - Google Patents

Method and system for eluting cells Download PDF

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Publication number
WO2005079531A3
WO2005079531A3 PCT/US2005/005490 US2005005490W WO2005079531A3 WO 2005079531 A3 WO2005079531 A3 WO 2005079531A3 US 2005005490 W US2005005490 W US 2005005490W WO 2005079531 A3 WO2005079531 A3 WO 2005079531A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
eluting cells
eluting
materials
solution
Prior art date
Application number
PCT/US2005/005490
Other languages
French (fr)
Other versions
WO2005079531A2 (en
Inventor
James P Landers
Jerome P Ferrance
Jessica C Voorhees
Kiev R Blasier
Original Assignee
Univ Virginia
James P Landers
Jerome P Ferrance
Jessica C Voorhees
Kiev R Blasier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Virginia, James P Landers, Jerome P Ferrance, Jessica C Voorhees, Kiev R Blasier filed Critical Univ Virginia
Publication of WO2005079531A2 publication Critical patent/WO2005079531A2/en
Publication of WO2005079531A3 publication Critical patent/WO2005079531A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition

Abstract

The present invention relates to method and system for eluting cells from materials for analysis and diagnostic examination by means of a swab, clothing items, or the like. The method includes incubating the cell-containing material in a solution that degrades the material while maintaining the cells or biological materials of interest, especially polynucleotides, such as DNA and/or RNA, intact. Preferably, the solution contains at least one enzyme for degrading the material. A system for practicing the method is also disclosed.
PCT/US2005/005490 2004-02-19 2005-02-22 Method and system for eluting cells WO2005079531A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54608004P 2004-02-19 2004-02-19
US60/546,080 2004-02-19

Publications (2)

Publication Number Publication Date
WO2005079531A2 WO2005079531A2 (en) 2005-09-01
WO2005079531A3 true WO2005079531A3 (en) 2006-12-14

Family

ID=34886237

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/005490 WO2005079531A2 (en) 2004-02-19 2005-02-22 Method and system for eluting cells

Country Status (1)

Country Link
WO (1) WO2005079531A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102187198A (en) 2008-08-15 2011-09-14 代表亚利桑那大学的亚利桑那校董会 Porous materials for biological sample collection
US10519434B2 (en) 2012-07-13 2019-12-31 Diomics Corporation Biologic sample collection devices and methods of production and use thereof
EP3179912B1 (en) 2014-08-15 2020-10-14 Diomics Corporation Films for biologic analyte collection and analysis and methods of production
WO2016048829A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US11207677B2 (en) 2018-03-07 2021-12-28 University Of Virginia Patent Foundation Devices, systems, and methods for detecting substances

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5603351A (en) * 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5623415A (en) * 1995-02-16 1997-04-22 Smithkline Beecham Corporation Automated sampling and testing of biological materials
US6184011B1 (en) * 1999-03-22 2001-02-06 Cbd Technologies, Ltd Method of releasing solid matrix affinity adsorbed particulates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5623415A (en) * 1995-02-16 1997-04-22 Smithkline Beecham Corporation Automated sampling and testing of biological materials
US5603351A (en) * 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US6184011B1 (en) * 1999-03-22 2001-02-06 Cbd Technologies, Ltd Method of releasing solid matrix affinity adsorbed particulates

Also Published As

Publication number Publication date
WO2005079531A2 (en) 2005-09-01

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