WO2005072456A3 - Single use lyophilized enzymatic reagents, and kits and methods for using same - Google Patents

Single use lyophilized enzymatic reagents, and kits and methods for using same Download PDF

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Publication number
WO2005072456A3
WO2005072456A3 PCT/US2005/003240 US2005003240W WO2005072456A3 WO 2005072456 A3 WO2005072456 A3 WO 2005072456A3 US 2005003240 W US2005003240 W US 2005003240W WO 2005072456 A3 WO2005072456 A3 WO 2005072456A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
single use
kits
same
dna
Prior art date
Application number
PCT/US2005/003240
Other languages
French (fr)
Other versions
WO2005072456A2 (en
Inventor
Thomas David Reed
Original Assignee
Gerard Biotech Llc
Thomas David Reed
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gerard Biotech Llc, Thomas David Reed filed Critical Gerard Biotech Llc
Publication of WO2005072456A2 publication Critical patent/WO2005072456A2/en
Publication of WO2005072456A3 publication Critical patent/WO2005072456A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A single use reagent article which comprises: (a) a holder; and a premeasured single use quantity of at least one lyophilized enzyme selected from the group consisting of nucleases, proteases, agarases and mixtures thereof associated with the holder. This reagent article can be used in a wide variety of biological kits and biological methods, such as molecular biological kits and methods for isolating DNA, including genomic DNA and DNA in agarose gels, where a known quantity of enzyme is needed having relatively consistent and stable activity. The reagent article is relatively easy to use and can minimize potential errors to due to user variability.
PCT/US2005/003240 2004-01-28 2005-01-28 Single use lyophilized enzymatic reagents, and kits and methods for using same WO2005072456A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48196304P 2004-01-28 2004-01-28
US60/481,963 2004-01-28

Publications (2)

Publication Number Publication Date
WO2005072456A2 WO2005072456A2 (en) 2005-08-11
WO2005072456A3 true WO2005072456A3 (en) 2005-09-29

Family

ID=34825854

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/003240 WO2005072456A2 (en) 2004-01-28 2005-01-28 Single use lyophilized enzymatic reagents, and kits and methods for using same

Country Status (1)

Country Link
WO (1) WO2005072456A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114634925A (en) * 2020-12-15 2022-06-17 深圳市帝迈生物技术有限公司 Nucleic acid extraction kit and method for extracting nucleic acid
CN115792231B (en) * 2022-11-04 2023-07-25 中拓生物有限公司 DNase I biosensor based on thrombin aptamer-regulated enzyme cascade reaction

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5403926A (en) * 1989-12-19 1995-04-04 The United States Of America As Represented By The Department Of Health And Human Services Hepatocellular carcinoma oncogene
US5869310A (en) * 1996-06-03 1999-02-09 Promega Corporation Isolated agarase enzyme from flavobacterium sp. strain NR19, cloned genes therefor, and expression thereof in transformed host cells
US6300068B1 (en) * 1997-05-02 2001-10-09 Biomerieux Vitek, Inc. Nucleic acid assays
US6548256B2 (en) * 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5403926A (en) * 1989-12-19 1995-04-04 The United States Of America As Represented By The Department Of Health And Human Services Hepatocellular carcinoma oncogene
US5869310A (en) * 1996-06-03 1999-02-09 Promega Corporation Isolated agarase enzyme from flavobacterium sp. strain NR19, cloned genes therefor, and expression thereof in transformed host cells
US6300068B1 (en) * 1997-05-02 2001-10-09 Biomerieux Vitek, Inc. Nucleic acid assays
US6548256B2 (en) * 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit

Also Published As

Publication number Publication date
WO2005072456A2 (en) 2005-08-11

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