WO2005071401A3 - Dosage multiplex homogene pour des cibles d'acides nucleiques - Google Patents

Dosage multiplex homogene pour des cibles d'acides nucleiques Download PDF

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Publication number
WO2005071401A3
WO2005071401A3 PCT/US2005/001378 US2005001378W WO2005071401A3 WO 2005071401 A3 WO2005071401 A3 WO 2005071401A3 US 2005001378 W US2005001378 W US 2005001378W WO 2005071401 A3 WO2005071401 A3 WO 2005071401A3
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WIPO (PCT)
Prior art keywords
nucleic acid
acid targets
multiplex assay
combination
homogeneous
Prior art date
Application number
PCT/US2005/001378
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English (en)
Other versions
WO2005071401A2 (fr
Inventor
Venkatakrishna Shyamala
Steve H Nguyen
Original Assignee
Chiron Corp
Venkatakrishna Shyamala
Steve H Nguyen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp, Venkatakrishna Shyamala, Steve H Nguyen filed Critical Chiron Corp
Publication of WO2005071401A2 publication Critical patent/WO2005071401A2/fr
Publication of WO2005071401A3 publication Critical patent/WO2005071401A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Nanotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Materials Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention a trait à un procédé pour l'analyse simultanée, dans un format homogène, pour la détection de plusieurs séquences cibles d'acides nucléiques. L'invention utilise des sondes de capture conjuguées à des substrats qui sont diférenciables en fonction d'une signature spectrale unique en combinaison avec des sondes rapporteuses ayant une étiquette séparément détectable. En présence d'une séquence cible, des complexes détectables sont formés qui sont différenciables pour chaque combinaison de cible, sonde de capture et sonde rapporteuse.
PCT/US2005/001378 2004-01-15 2005-01-14 Dosage multiplex homogene pour des cibles d'acides nucleiques WO2005071401A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53697804P 2004-01-15 2004-01-15
US60/536,978 2004-01-15

Publications (2)

Publication Number Publication Date
WO2005071401A2 WO2005071401A2 (fr) 2005-08-04
WO2005071401A3 true WO2005071401A3 (fr) 2005-12-01

Family

ID=34807070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/001378 WO2005071401A2 (fr) 2004-01-15 2005-01-14 Dosage multiplex homogene pour des cibles d'acides nucleiques

Country Status (1)

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WO (1) WO2005071401A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2402465B1 (fr) 2004-07-13 2014-11-19 Gen-Probe Incorporated Procédé et trousse pour la détection d'acide nucléique du virus de l'hépatite A
ES2374788T3 (es) 2005-12-23 2012-02-22 Nanostring Technologies, Inc. Nanoinformadores y métodos para su producción y uso.
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US7534588B2 (en) * 2007-05-25 2009-05-19 Asiagen Corporation Methods, kits and polynucleotides for simultaneously diagnosing viruses
EP2488876B1 (fr) * 2009-10-13 2017-03-01 Nanostring Technologies, Inc Détection de protéine par l'intermédiaire de nanorapporteurs
JP6083849B2 (ja) * 2012-07-20 2017-02-22 国立大学法人静岡大学 試料中の標的物質を検出又は定量する方法及びキット
US10011811B2 (en) 2013-11-25 2018-07-03 Vericel Corporation Cell seeding device and method
CN103616513A (zh) * 2013-12-06 2014-03-05 苏州长光华医生物医学工程有限公司 乙型肝炎病毒e抗原测定试剂盒以及检测方法
CN103630531A (zh) * 2013-12-06 2014-03-12 苏州长光华医生物医学工程有限公司 乙型肝炎表面抗原测定试剂盒以及检测方法
CN104090101A (zh) * 2014-07-21 2014-10-08 威海威高生物科技有限公司 人类免疫缺陷病毒抗体检测试剂盒及其制备方法
CN111909932B (zh) * 2020-07-17 2023-06-20 南方医科大学 一种原位检测外泌体多重microRNA的纳米金荧光探针及其制备方法与应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002785A2 (fr) * 1986-10-14 1988-04-21 Beckman Instruments, Inc. Technique amelioree d'hybridation d'acide nucleique et kit relatif
US5516635A (en) * 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2002040698A2 (fr) * 2000-11-17 2002-05-23 Surromed, Inc. Analyse de marqueurs biologiques de facteurs solubles
WO2003002753A2 (fr) * 2001-06-28 2003-01-09 Chiron Corporation Methodes diagnostiques pour la detection du parvovirus b19
WO2003031934A2 (fr) * 2001-10-09 2003-04-17 Chiron Corporation Identification d'oligonucleotides pour la capture, la detection et l'evaluation quantitative de l'adn viral de l'hepatite b

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002785A2 (fr) * 1986-10-14 1988-04-21 Beckman Instruments, Inc. Technique amelioree d'hybridation d'acide nucleique et kit relatif
US5516635A (en) * 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US6268147B1 (en) * 1998-11-02 2001-07-31 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
WO2002040698A2 (fr) * 2000-11-17 2002-05-23 Surromed, Inc. Analyse de marqueurs biologiques de facteurs solubles
WO2003002753A2 (fr) * 2001-06-28 2003-01-09 Chiron Corporation Methodes diagnostiques pour la detection du parvovirus b19
WO2003031934A2 (fr) * 2001-10-09 2003-04-17 Chiron Corporation Identification d'oligonucleotides pour la capture, la detection et l'evaluation quantitative de l'adn viral de l'hepatite b

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SPIRO A ET AL: "A BEAD-BASED METHOD FOR MULTIPLEXED IDENTIFICATION AND QUANTITATION OF DNA SEQUENCES USING FLOW CYTOMETRY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 66, no. 10, October 2000 (2000-10-01), pages 4258 - 4265, XP001069133, ISSN: 0099-2240 *

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Publication number Publication date
WO2005071401A2 (fr) 2005-08-04

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