WO2005068622A3 - Mixtures of binding proteins - Google Patents

Mixtures of binding proteins Download PDF

Info

Publication number
WO2005068622A3
WO2005068622A3 PCT/NL2005/000036 NL2005000036W WO2005068622A3 WO 2005068622 A3 WO2005068622 A3 WO 2005068622A3 NL 2005000036 W NL2005000036 W NL 2005000036W WO 2005068622 A3 WO2005068622 A3 WO 2005068622A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding proteins
cells
different target
polypeptide chain
single polypeptide
Prior art date
Application number
PCT/NL2005/000036
Other languages
French (fr)
Other versions
WO2005068622A2 (en
Inventor
Ton Logtenberg
Hendricus Renerus J Hoogenboom
Original Assignee
Merus B V
Ton Logtenberg
Hendricus Renerus J Hoogenboom
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merus B V, Ton Logtenberg, Hendricus Renerus J Hoogenboom filed Critical Merus B V
Priority to JP2006550975A priority Critical patent/JP5912211B2/en
Priority to AU2005205412A priority patent/AU2005205412B2/en
Priority to EP05704566.8A priority patent/EP1737971B1/en
Priority to NZ548828A priority patent/NZ548828A/en
Priority to CA2554054A priority patent/CA2554054C/en
Priority to ES05704566.8T priority patent/ES2646560T3/en
Priority to DK05704566.8T priority patent/DK1737971T3/en
Publication of WO2005068622A2 publication Critical patent/WO2005068622A2/en
Publication of WO2005068622A3 publication Critical patent/WO2005068622A3/en
Priority to US11/490,545 priority patent/US8268756B2/en
Priority to US13/200,972 priority patent/US9012371B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/20—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • C07K2318/20—Antigen-binding scaffold molecules wherein the scaffold is not an immunoglobulin variable region or antibody mimetics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides methods for the production of libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.
PCT/NL2005/000036 2004-01-20 2005-01-19 Mixtures of binding proteins WO2005068622A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP2006550975A JP5912211B2 (en) 2004-01-20 2005-01-19 Mixture of binding proteins
AU2005205412A AU2005205412B2 (en) 2004-01-20 2005-01-19 Mixtures of binding proteins
EP05704566.8A EP1737971B1 (en) 2004-01-20 2005-01-19 Mixtures of binding proteins
NZ548828A NZ548828A (en) 2004-01-20 2005-01-19 Mixtures of binding proteins
CA2554054A CA2554054C (en) 2004-01-20 2005-01-19 Mixtures of binding proteins
ES05704566.8T ES2646560T3 (en) 2004-01-20 2005-01-19 Binding Protein Blends
DK05704566.8T DK1737971T3 (en) 2004-01-20 2005-01-19 MIXTURES OF BINDING PROTEINS
US11/490,545 US8268756B2 (en) 2004-01-20 2006-07-20 Mixture of binding proteins
US13/200,972 US9012371B2 (en) 2004-01-20 2011-10-05 Mixtures of binding proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04075170.3 2004-01-20
EP04075170 2004-01-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/490,545 Continuation US8268756B2 (en) 2004-01-20 2006-07-20 Mixture of binding proteins

Publications (2)

Publication Number Publication Date
WO2005068622A2 WO2005068622A2 (en) 2005-07-28
WO2005068622A3 true WO2005068622A3 (en) 2006-03-02

Family

ID=34778197

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2005/000036 WO2005068622A2 (en) 2004-01-20 2005-01-19 Mixtures of binding proteins

Country Status (9)

Country Link
US (2) US8268756B2 (en)
EP (1) EP1737971B1 (en)
JP (2) JP5912211B2 (en)
AU (1) AU2005205412B2 (en)
CA (1) CA2554054C (en)
DK (1) DK1737971T3 (en)
ES (1) ES2646560T3 (en)
NZ (1) NZ548828A (en)
WO (1) WO2005068622A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ537277A (en) 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
CA2527694C (en) 2003-05-30 2015-07-14 Hendricus Renerus Jacobus Mattheus Hoogenboom Fab library for the preparation of anti vegf and anti rabies virus fabs
EP1737971B1 (en) 2004-01-20 2017-08-16 Merus N.V. Mixtures of binding proteins
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
LT2285408T (en) * 2008-06-05 2019-01-25 Ablynx N.V. Amino acid sequences directed against envelope proteins of a virus and polypeptides comprising the same for the treatment of viral diseases
RU2559524C2 (en) 2008-06-27 2015-08-10 Мерюс Б.В. Antibody-producing non-human mammals
EP2159291A1 (en) 2008-09-01 2010-03-03 Agendia B.V. Means and method for determining tumor cell percentage in a sample
US8551733B2 (en) 2009-02-09 2013-10-08 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
AU2010255638B2 (en) 2009-06-05 2014-10-16 Ablynx Nv Monovalent, bivalent and trivalent anti human respiratory syncytial virus (hRSV) nanobody constructs for the prevention and/or treatment of respiratory tract infections
JP2011147403A (en) * 2010-01-22 2011-08-04 Hitachi High-Technologies Corp Bacterial test apparatus and bacterial test method
AU2011326164B2 (en) 2010-11-08 2016-05-05 The Board Of Trustees Of The Leland Stanford Junior University Fusion proteins comprising an engineered knottin peptide and uses thereof
WO2012122512A1 (en) * 2011-03-10 2012-09-13 Hco Antibody, Inc. Recombinant production of mixtures of single chain antibodies
CA2791109C (en) 2011-09-26 2021-02-16 Merus B.V. Generation of binding molecules
EA035344B1 (en) 2012-04-20 2020-05-29 Мерюс Н.В. Method for producing two antibodies from a single host cell
WO2016164305A1 (en) * 2015-04-06 2016-10-13 Subdomain, Llc De novo binding domain containing polypeptides and uses thereof
US11464803B2 (en) 2017-11-14 2022-10-11 Arcellx, Inc. D-domain containing polypeptides and uses thereof
CN111511383A (en) 2017-11-14 2020-08-07 阿奇利克斯股份有限公司 Multifunctional immune cell therapy
AU2018369639A1 (en) 2017-11-14 2020-04-23 Arcellx, Inc. D-domain containing polypeptides and uses thereof
WO2021163490A1 (en) * 2020-02-14 2021-08-19 University Of Washington Targeted depletion of bacteria from mixed populations through programmable cell-cell adhesion
EP4144898A1 (en) * 2021-09-07 2023-03-08 New/era/mabs GmbH Method for selecting or screening antibodies from an antibody library

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002610A1 (en) * 1992-07-17 1994-02-03 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
WO2002096948A2 (en) * 2001-01-29 2002-12-05 Idec Pharmaceuticals Corporation Engineered tetravalent antibodies and methods of use
WO2003048306A2 (en) * 2001-11-16 2003-06-12 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
US20030194403A1 (en) * 2001-06-13 2003-10-16 Genmab, Inc. Human monoclonal antibodies to epidermal growth factor receptor (EGFR)
WO2004106375A1 (en) * 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs

Family Cites Families (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4399216A (en) * 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4634665A (en) * 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4599311A (en) * 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
DE3785186T2 (en) 1986-09-02 1993-07-15 Enzon Lab Inc BINDING MOLECULE WITH SINGLE POLYPEPTIDE CHAIN.
US4801687A (en) 1986-10-27 1989-01-31 Bioprobe International, Inc. Monoclonal antibody purification process using protein A
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
NZ226694A (en) 1987-10-28 1994-04-27 Oncogen Human immunoglobulin produced by recombinant dna techniques
ES2035317T5 (en) * 1987-11-09 1998-03-16 Becton Dickinson Co METHOD FOR ANALYZING HEMATOPOYETIC CELLS IN A SAMPLE.
JP3040121B2 (en) 1988-01-12 2000-05-08 ジェネンテク，インコーポレイテッド Methods of treating tumor cells by inhibiting growth factor receptor function
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
WO1990002809A1 (en) 1988-09-02 1990-03-22 Protein Engineering Corporation Generation and selection of recombinant varied binding proteins
ES2062364T3 (en) 1989-06-08 1994-12-16 Wistar Inst A METHOD OF PREPARING A COMPOSITION FOR TREATMENT AFTER EXPOSURE TO RABIES INFECTION.
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
AU656720B2 (en) 1989-12-01 1995-02-16 Gene Pharming Europe Bv Production of recombinant polypeptides by bovine species and transgenic methods
DE4006630A1 (en) 1990-03-03 1991-09-12 Behringwerke Ag HUMANE MONOCLONAL ANTIBODIES AGAINST RABBIT VIRUSES, THEIR PRODUCTION AND USE
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
JPH06508511A (en) 1990-07-10 1994-09-29 ケンブリッジ　アンティボディー　テクノロジー　リミティド Method for producing specific binding pair members
EP0469897A3 (en) 1990-07-31 1992-11-19 The Wistar Institute Engineered antibodies
IE76732B1 (en) * 1990-08-07 1997-11-05 Becton Dickinson Co One step test for absolute counts
GB9022543D0 (en) 1990-10-17 1990-11-28 Wellcome Found Antibody production
ATE164395T1 (en) 1990-12-03 1998-04-15 Genentech Inc METHOD FOR ENRICHMENT OF PROTEIN VARIANTS WITH MODIFIED BINDING PROPERTIES
AU1545692A (en) 1991-03-01 1992-10-06 Protein Engineering Corporation Process for the development of binding mini-proteins
DK0724639T3 (en) 1991-03-29 2001-03-05 Genentech Inc Methods for selecting recombinant host cells expressing high levels of a desired protein
DE69233367T2 (en) 1991-04-10 2005-05-25 The Scripps Research Institute, La Jolla LIBRARIES OF HETERODIMERIC RECEPTORS BY PHAGEMIDES
US5637481A (en) 1993-02-01 1997-06-10 Bristol-Myers Squibb Company Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell
DE4122599C2 (en) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid for screening antibodies
NZ243558A (en) 1991-07-15 1994-06-27 Wellcome Found Recombinant primate antibodies and their production
EP0552108B1 (en) * 1992-01-17 1999-11-10 Lakowicz, Joseph R. Energy transfer phase-modulation fluoro-immunoassay
US7067284B1 (en) 1992-01-27 2006-06-27 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
US5667988A (en) 1992-01-27 1997-09-16 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
SE9201984D0 (en) * 1992-06-29 1992-06-29 Pharmacia Biosensor Ab IMPROVEMENT IN OPTICAL ASSAYS
JP3832847B2 (en) * 1992-07-17 2006-10-11 ダナ−ファーバー キャンサー インスティテュート Intracellular binding method of target molecule
EP0669986B1 (en) 1992-11-13 2003-04-09 Idec Pharmaceuticals Corporation Fully impaired consensus kozac sequences for mammalian expression
AU6796094A (en) 1993-04-29 1994-11-21 Raymond Hamers Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of (camelidae)
US5693506A (en) 1993-11-16 1997-12-02 The Regents Of The University Of California Process for protein production in plants
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
EP0739412B1 (en) 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS
DK1231268T3 (en) 1994-01-31 2005-11-21 Univ Boston Polyclonal antibody libraries
FR2717187B1 (en) * 1994-03-10 1996-05-31 Transgene Sa Combined use of two expression cassettes for the production of a protein of interest.
US6080560A (en) 1994-07-25 2000-06-27 Monsanto Company Method for producing antibodies in plant cells
JPH08116978A (en) * 1994-10-18 1996-05-14 Nisshinbo Ind Inc Preparation of antibody fab library
ATE369423T1 (en) 1994-12-30 2007-08-15 Planet Biotechnology Inc METHOD FOR PRODUCING IMMUNOGLOBULINS CONTAINING PROTECTIVE PROTEINS AND USE THEREOF
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6265150B1 (en) 1995-06-07 2001-07-24 Becton Dickinson & Company Phage antibodies
US5783186A (en) 1995-12-05 1998-07-21 Amgen Inc. Antibody-induced apoptosis
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6825396B2 (en) 1996-06-12 2004-11-30 Board Of Trustees Operating Michigan State University Methods for tissue specific synthesis of protein in eggs of transgenic hens
ATE230850T1 (en) 1996-10-08 2003-01-15 Bisys B V U METHOD AND MEANS FOR SELECTING PEPTIDES AND PROTEINS WITH SPECIFIC AFFINITY FOR A TARGET MOLECULE
GB9621113D0 (en) 1996-10-10 1996-11-27 Univ Southampton Transgenic fish
EP0971946B1 (en) * 1997-01-21 2006-07-05 The General Hospital Corporation Selection of proteins using rna-protein fusions
NZ337495A (en) 1997-03-06 2001-06-29 Infigen Inc Bovine primordial germ cells and their use in cloning
ATE296356T1 (en) 1997-03-14 2005-06-15 Biogen Idec Inc METHOD FOR SPECIFIC INTEGRATION OF GENES IN MAMMAL CELLS BY HOMOLOGOUS RECOMBINATION, AND VECTORS FOR IMPLEMENTING IT
US6080912A (en) 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals
CA2286330C (en) 1997-04-07 2008-06-10 Genentech, Inc. Anti-vegf antibodies
US20030207346A1 (en) 1997-05-02 2003-11-06 William R. Arathoon Method for making multispecific antibodies having heteromultimeric and common components
IL132560A0 (en) 1997-05-02 2001-03-19 Genentech Inc A method for making multispecific antibodies having heteromultimeric and common components
GB9722131D0 (en) 1997-10-20 1997-12-17 Medical Res Council Method
EP1027439B1 (en) * 1997-10-27 2010-03-17 Bac Ip B.V. Multivalent antigen-binding proteins
US6413776B1 (en) 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
ATE536417T1 (en) 1999-04-15 2011-12-15 Crucell Holland Bv PRODUCTION OF RECOMBINANT PROTEINS IN A HUMAN CELL WITH AT LEAST ONE E1 ADENOVIRUS PROTEIN
PT2067788E (en) 1999-05-18 2015-10-29 Dyax Corp Fab fragment libraries and methods for their use
US6472147B1 (en) 1999-05-25 2002-10-29 The Scripps Research Institute Methods for display of heterodimeric proteins on filamentous phage using pVII and pIX, compositions, vectors and combinatorial libraries
US6833268B1 (en) 1999-06-10 2004-12-21 Abgenix, Inc. Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions
WO2001019394A2 (en) 1999-09-15 2001-03-22 Therapeutic Human Polyclonals, Inc. Immunotherapy with substantially human polyclonal antibody preparations purified from genetically engineered birds
US6180357B1 (en) 1999-10-08 2001-01-30 Arius Research, Inc. Individualized patient-specific anti-cancer antibodies
WO2001027279A1 (en) 1999-10-12 2001-04-19 Cambridge Antibody Technology Human anti-adipocyte monoclonal antibodies and their use
EP2278021A3 (en) 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expression vectors, transfection systems, and method of use thereof
GB9928787D0 (en) * 1999-12-03 2000-02-02 Medical Res Council Direct screening method
DE60038132T2 (en) 1999-12-27 2009-04-02 Crucell Holland B.V. Antibody to Ep-Cam
CA2401965A1 (en) 2000-02-29 2001-09-07 Auburn University Production of antibodies in transgenic plastids
WO2001080883A1 (en) 2000-04-26 2001-11-01 Elusys Therapeutics, Inc. Bispecific molecules and uses thereof
DE60136174D1 (en) 2000-05-16 2008-11-27 Univ Jefferson RABIESVIRUS-SPECIFIC NEUTRALIZING HUMAN MONOCLONAL ANTIBODIES AND NUCLEIC ACIDS AND RELATED METHODS
KR20080074231A (en) * 2000-06-29 2008-08-12 아보트 러보러터리즈 Dual specificity antibodies and methods of making and using
EP1184458A1 (en) 2000-08-28 2002-03-06 U-BISys B.V. Differentially expressed CD46 epitopes, proteinaceous molecules capable of binding thereto, and uses thereof
US7737258B2 (en) * 2000-10-18 2010-06-15 Sloan-Kettering Institute For Cancer Research Uses of monoclonal antibody 8H9
JP3523245B1 (en) 2000-11-30 2004-04-26 メダレックス，インコーポレーテッド Transgenic chromosome-introduced rodents for the production of human antibodies
FR2817875B1 (en) 2000-12-07 2005-03-18 Technopharm PROCESS FOR THE PREPARATION OF A HUMAN MONOCLONAL ANTIBODY, FRAGMENTS THEREOF OR ANTIBODIES COMPRISING SUCH FRAGMENTS, THE ANTIBODIES THUS OBTAINED AND THEIR USE
WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
DE60233049D1 (en) 2001-01-26 2009-09-03 Selexis Sa MATRIX MOUNTING REGIONS AND METHOD OF USE THEREOF
GB0110029D0 (en) 2001-04-24 2001-06-13 Grosveld Frank Transgenic animal
IL159225A0 (en) * 2001-06-13 2004-06-01 Genmab As Human monoclonal antibodies to epidermal growth factor receptor (egfr)
DE60237282D1 (en) * 2001-06-28 2010-09-23 Domantis Ltd DOUBLE-SPECIFIC LIGAND AND ITS USE
ATE466942T1 (en) 2001-07-04 2010-05-15 Chromagenics Bv DNS SEQUENCES WITH ANTI-REPRESSOR ACTIVITY
NZ531291A (en) 2001-08-21 2006-02-24 Univ Jefferson Recombinant anti rabies antibodies MAb JB.1 and Mab 57 and compositions and methods for making and using the same
DE60226486D1 (en) * 2001-11-30 2008-06-19 Ca Nat Research Council SELF-ARRANGING MOLECULES
US7244592B2 (en) * 2002-03-07 2007-07-17 Dyax Corp. Ligand screening and discovery
CA2488441C (en) 2002-06-03 2015-01-27 Genentech, Inc. Synthetic antibody phage libraries
ES2362273T3 (en) * 2002-06-14 2011-06-30 Chromagenics B.V. METHOD FOR THE SIMULTANEOUS PRODUCTION OF MULTIPLE PROTEINS; VECTORS AND CELLS FOR USE IN THE SAME.
US20080241166A1 (en) * 2002-06-28 2008-10-02 Domantis Limited Ligands that bind a receptor
NZ537277A (en) 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
EP1737971B1 (en) 2004-01-20 2017-08-16 Merus N.V. Mixtures of binding proteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002610A1 (en) * 1992-07-17 1994-02-03 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
WO2002096948A2 (en) * 2001-01-29 2002-12-05 Idec Pharmaceuticals Corporation Engineered tetravalent antibodies and methods of use
US20030194403A1 (en) * 2001-06-13 2003-10-16 Genmab, Inc. Human monoclonal antibodies to epidermal growth factor receptor (EGFR)
WO2003048306A2 (en) * 2001-11-16 2003-06-12 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
WO2004106375A1 (en) * 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HOLLIGER P ET AL: "DIABODIES: SMALL BIVALENT AND BISPECIFIC ANTIBODY FRAGMENTS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 90, 1 July 1993 (1993-07-01), pages 6444 - 6448, XP002008022, ISSN: 0027-8424 *
KORTT A A ET AL: "Dimeric and trimeric antibodies: high avidity scFvs for cancer targeting", BIOMOLECULAR ENGINEERING, ELSEVIER, NEW YORK, NY, US, vol. 18, no. 3, 15 October 2001 (2001-10-15), pages 95 - 108, XP004305907, ISSN: 1389-0344 *

Also Published As

Publication number Publication date
DK1737971T3 (en) 2017-11-13
JP5912211B2 (en) 2016-04-27
AU2005205412B2 (en) 2011-08-11
US20070059766A1 (en) 2007-03-15
US8268756B2 (en) 2012-09-18
JP5685274B2 (en) 2015-03-18
CA2554054A1 (en) 2005-07-28
JP2013143939A (en) 2013-07-25
EP1737971A2 (en) 2007-01-03
JP2007520218A (en) 2007-07-26
CA2554054C (en) 2013-06-04
US20120058907A1 (en) 2012-03-08
EP1737971B1 (en) 2017-08-16
NZ548828A (en) 2009-07-31
US9012371B2 (en) 2015-04-21
ES2646560T3 (en) 2017-12-14
AU2005205412A1 (en) 2005-07-28
WO2005068622A2 (en) 2005-07-28

Similar Documents

Publication Publication Date Title
WO2005068622A3 (en) Mixtures of binding proteins
WO2004056981A3 (en) Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
WO2003000941A3 (en) Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
WO2006053021A3 (en) Methods for engineering polypeptide variants via somatic hypermutation and polypeptides made thereby
ATE541283T1 (en) METHOD FOR MIXING COLORS IN A DISPLAY
WO2007077028A3 (en) Antibodies directed to her-3 and uses thereof
WO2006073839A3 (en) Acid fungal proteases
WO2002095014A3 (en) Polypeptides having cellobiase activity and polynucleotides encoding same
WO2005116213A3 (en) Directed evolution of proteins
WO2007002261A3 (en) IL-1β BINDING ANTIBODIES AND FRAGMENTS THEREOF
WO2006047469A8 (en) Polypeptides having lipase activity and polynucleotides encoding same
WO2002031134A3 (en) Novel serine protease genes related to dppiv
WO2006091094A3 (en) Hemoglobin overexpression in fungal fermentations
WO2004009618A3 (en) Recombinant production of mixtures of antibodies
WO2006048459A3 (en) Selection of host cells expressing protein at high levels
ATE443074T1 (en) PREPARATIONS AND METHODS FOR USE IN RECOMBINATORY CLONING OF NUCLEIC ACIDS
WO2006041934A3 (en) Methods and compositions for improving recombinant protein production
WO2002068605A3 (en) Non-oligomerizing tandem fluorescent proteins
WO2007005604A3 (en) Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof
WO2005062918A3 (en) Methods of treating asthma
WO2003089598A3 (en) Polypeptides having xyloglucanase activity and nucleic acids encoding same
JP2012514977A5 (en)
WO2011156539A3 (en) Compositions and methods for protein production
WO2005112997A3 (en) Composition and method for introduction of dna directed or direct rna interference sequence into targeted cells and tissues
WO2006005032A3 (en) Polypeptides having alpha-glucosidase activity and polynucleotides encoding same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2554054

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 11490545

Country of ref document: US

Ref document number: 2006550975

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Ref document number: DE

WWE Wipo information: entry into national phase

Ref document number: 2005205412

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 548828

Country of ref document: NZ

ENP Entry into the national phase

Ref document number: 2005205412

Country of ref document: AU

Date of ref document: 20050119

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2005205412

Country of ref document: AU

REEP Request for entry into the european phase

Ref document number: 2005704566

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2005704566

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2005704566

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 11490545

Country of ref document: US