WO2005066639A3 - Affinity displacement avidity effect protein resolution methods - Google Patents

Affinity displacement avidity effect protein resolution methods Download PDF

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Publication number
WO2005066639A3
WO2005066639A3 PCT/US2004/041673 US2004041673W WO2005066639A3 WO 2005066639 A3 WO2005066639 A3 WO 2005066639A3 US 2004041673 W US2004041673 W US 2004041673W WO 2005066639 A3 WO2005066639 A3 WO 2005066639A3
Authority
WO
WIPO (PCT)
Prior art keywords
avidity effect
resolution methods
effect protein
protein resolution
affinity
Prior art date
Application number
PCT/US2004/041673
Other languages
French (fr)
Other versions
WO2005066639A2 (en
Inventor
Timothy A J Haystead
Original Assignee
Serenex Inc
Timothy A J Haystead
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serenex Inc, Timothy A J Haystead filed Critical Serenex Inc
Publication of WO2005066639A2 publication Critical patent/WO2005066639A2/en
Publication of WO2005066639A3 publication Critical patent/WO2005066639A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compositions and methods to predict the Kd values of a compound for protein targets within a ligand binding proteome are provided. More specifically, the method relates to measuring the avidity effect of a ligand upon the formation of a complex of a protein and a compound of interest, then determining the affinity of the compound of interest for the protein from the measurement.
PCT/US2004/041673 2003-12-23 2004-12-10 Affinity displacement avidity effect protein resolution methods WO2005066639A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53212203P 2003-12-23 2003-12-23
US60/532,122 2003-12-23

Publications (2)

Publication Number Publication Date
WO2005066639A2 WO2005066639A2 (en) 2005-07-21
WO2005066639A3 true WO2005066639A3 (en) 2005-08-18

Family

ID=34748789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/041673 WO2005066639A2 (en) 2003-12-23 2004-12-10 Affinity displacement avidity effect protein resolution methods

Country Status (1)

Country Link
WO (1) WO2005066639A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010047086A1 (en) * 2000-02-23 2001-11-29 Cramer Steven M. High throughput screening of potential displacer molecules
WO2003052382A2 (en) * 2001-12-18 2003-06-26 Serenex, Inc. Integrated protein affinity capture centrifugation device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010047086A1 (en) * 2000-02-23 2001-11-29 Cramer Steven M. High throughput screening of potential displacer molecules
WO2003052382A2 (en) * 2001-12-18 2003-06-26 Serenex, Inc. Integrated protein affinity capture centrifugation device

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ALMAGOR H ET AL: "ANALYTICAL DETERMINATION OF RECEPTOR-LIGAND DISSOCIATION CONSTANTS OF TWO POPULATIONS OF RECEPTORS FROM DISPLACEMENT CURVES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 87, no. 17, 1990, pages 6482 - 6486, XP002333734, ISSN: 0027-8424 *
DE CRESCENZO G ET AL: "Transforming Growth Factor-beta (TGF-beta) Binding to the Extracellular Domain of the Type II TGF-beta Receptor: Receptor Capture on a Biosensor Surface Using a New Coiled-coil Capture System Demonstrates that Avidity Contributes Significantly to High Affinity Binding", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 328, no. 5, 16 May 2003 (2003-05-16), pages 1173 - 1183, XP004454244, ISSN: 0022-2836 *
DMITRIEV D A ET AL: "Kinetic analysis of interactions between bispecific monoclonal antibodies and immobilized antigens using a resonant mirror biosensor", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 280, no. 1-2, September 2003 (2003-09-01), pages 183 - 1202, XP004455348, ISSN: 0022-1759 *
GRAVES PAUL R ET AL: "Discovery of novel targets of quinoline drugs in the human purine binding proteome", MOLECULAR PHARMACOLOGY, BALTIMORE, MD, US, vol. 62, no. 6, December 2002 (2002-12-01), pages 1364 - 1372, XP002297994, ISSN: 0026-895X *
KITOV PAVEL I ET AL: "On the nature of the multivalency effect: A thermodynamic model.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 125, no. 52, 3 December 2003 (2003-12-03), pages 16271 - 16284, XP002333735, ISSN: 0002-7863 *
NILSSON J ET AL: "COMPETITIVE ELUTION OF PROTEIN A FUSION PROTEINS ALLOWS SPECIFIC RECOVERY UNDER MILD CONDITIONS", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 224, no. 1, 15 August 1994 (1994-08-15), pages 103 - 108, XP000615210, ISSN: 0014-2956 *
ZHANG B ET AL: "Frontal affinity chromatography coupled to mass spectrometry for screening mixtures of enzyme inhibitors", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 299, no. 2, 15 December 2001 (2001-12-15), pages 173 - 182, XP002219168, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2005066639A2 (en) 2005-07-21

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