WO2005066358A1 - Procede permettant de determiner la concentration de virus dans un materiau biologique liquide - Google Patents

Procede permettant de determiner la concentration de virus dans un materiau biologique liquide Download PDF

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Publication number
WO2005066358A1
WO2005066358A1 PCT/RU2004/000003 RU2004000003W WO2005066358A1 WO 2005066358 A1 WO2005066358 A1 WO 2005066358A1 RU 2004000003 W RU2004000003 W RU 2004000003W WO 2005066358 A1 WO2005066358 A1 WO 2005066358A1
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WO
WIPO (PCT)
Prior art keywords
κleτοκ
cells
viρusοv
eleκτροdami
virus
Prior art date
Application number
PCT/RU2004/000003
Other languages
English (en)
Russian (ru)
Inventor
Talgat Salmanovich Bakirov
Vladimir Mikhailovich Generalov
Aleksandr Gavrilovich Durymanov
Ivan Leonidovich Zvolsky
Vladimir Sergeevich Toporkov
Larisa Nikolaevna Shishkina
Original Assignee
Gosudarstvenny Nauchny Tsentr Virusologii I Biotekhnologii 'vektor'
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gosudarstvenny Nauchny Tsentr Virusologii I Biotekhnologii 'vektor' filed Critical Gosudarstvenny Nauchny Tsentr Virusologii I Biotekhnologii 'vektor'
Priority to PCT/RU2004/000003 priority Critical patent/WO2005066358A1/fr
Publication of WO2005066358A1 publication Critical patent/WO2005066358A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination

Definitions

  • the method of separation of the concentration of species in a liquid biological material is subject to biology, virology and medicine.
  • m ⁇ zhe ⁇ by ⁇ is ⁇ lz ⁇ van ⁇ for ⁇ edeleniya ⁇ ntsen ⁇ atsii vi ⁇ us ⁇ v in sus ⁇ enziya ⁇ , na ⁇ ime ⁇ in ⁇ vi chel ⁇ ve ⁇ a and zhiv ⁇ ny ⁇ , v ⁇ vzvesya ⁇ ⁇ as ⁇ i ⁇ elny ⁇ and zhiv ⁇ ny ⁇ ⁇ le ⁇ , ba ⁇ e ⁇ y and ⁇ a ⁇ zhe in ⁇ myshlenn ⁇ m ⁇ izv ⁇ ds ⁇ ve for ⁇ n ⁇ lya chis ⁇ y ⁇ e ⁇ n ⁇ l ⁇ giches ⁇ i ⁇ zhid ⁇ s ⁇ ey.
  • the essence of the proposed method is that the labeled antibodies are not interconnected with the shared virus in the system.
  • the newly developed anti-virus complex is convertible to fixed state by the sequential addition of the band and the polish.
  • the consumer electronics complex is a polianon-policeman and is the receiver of the virus-policeman complex. It is possible to distribute the marking of the product in an unprofitable manner in a factory or in a newly developed complex after its use.
  • the shared concentrate of the metric is the basic initial concentrate of the shared virus and serves as a storage unit for the test product.
  • the disadvantage of this method is the high labor consumption and the duration of the analysis and the receipt of the original materials.
  • ⁇ ⁇ - ⁇
  • ⁇ is the number of fixed cells, the speed is faster or the same as the speed of the vehicle;
  • ⁇ - ⁇ bschee ⁇ liches ⁇ v ⁇ ⁇ le ⁇ , ts ⁇ avshi ⁇ in ⁇ le izme ⁇ eniya with ⁇ sleduyuschim ⁇ edeleniem ⁇ ntsen ⁇ atsii vi ⁇ us ⁇ v C.
  • ⁇ ⁇ mule C C ⁇ • ⁇ - ⁇ • to ⁇ ed ⁇ s ⁇ a ⁇ m s ⁇ s ⁇ ba- ⁇ i ⁇ a yavlyae ⁇ sya b ⁇ lsh ⁇ y ⁇ bem computing svyazanny ⁇ with tsi ⁇ v ⁇ y ⁇ b ⁇ ab ⁇ y vide ⁇ iz ⁇ b ⁇ azheny and ⁇ a ⁇ zhe ne ⁇ b ⁇ dim ⁇ s ⁇ ⁇ i ⁇ b ⁇ e ⁇ eniya Larger memory devices for processing and storage of information in electronic form.
  • the cage ( ⁇ ) remaining in the field of vision of the microscope between the electrodes of the measuring camera has the effect of interfering with electric cure 3-60.
  • the susceptibility of virious susceptible cells to susceptibility to viruses is eliminated in the absence of susceptibility to viability.
  • the above-mentioned experiments showed that the portable (uninfected) cells are less stable under conditions of a removable electric field than the infected ones.
  • ⁇ a ⁇ ig. 1 is a schematic of the measuring cell.
  • ⁇ a ⁇ ig. 2 The operating system is provided with a fair amount of support for the implementation of the process of the installation.
  • ⁇ a ⁇ ig. 3 The basic block diagram of an automatic installation for the implementation of the claimed method is illustrated.
  • the method may be implemented using the following technical devices. Us ⁇ ys ⁇ v ⁇ for ⁇ edeleniya ⁇ ntsen ⁇ atsii C.
  • the gap between the electrodes 2 and 3 in the experimental components is set in the range of 50-100 ⁇ m, and the thickness of the specified electric elements is 0.2-2 ⁇ m.
  • the device functions as follows.
  • the camera 1 is installed on the movable panel 8 at 5 and the camera indicated on it is indicated • Camera 1.
  • the camera is disconnected and the camera is damaged.
  • ⁇ is ⁇ chni ⁇ a 4 (gene ⁇ a ⁇ a) ⁇ e ⁇ emenn ⁇ g ⁇ na ⁇ yazheniya between ⁇ ymi ⁇ mi ⁇ ue ⁇ sya s ⁇ ednyaya na ⁇ yazhenn ⁇ s ⁇ ele ⁇ iches ⁇ g ⁇ ⁇ lya in ⁇ edela ⁇ ⁇ May 10 d ⁇ v ⁇ l ⁇ 10 b / m.
  • EXAMPLE 2 Separation of the concentration of viruses in the cell suspension. Separation of the concentration of viruses in the cartridge suspension, implements the following method. On a flat panel, a continuous number of holes in the moon is available from 5 to 15. The quantity of the moon is selected based on the apirical concentration of the virus.
  • Each hole is filled with 100 ⁇ l of cell suspension with concentration, for example, 1 million / ml. Further, in the first hole, in a row, 100 microns are added. viral suspension. The concentration of the viruses, starting from the first hole, changes (counts) with the dilution step selected at the beginning, for example, in (2, 10) times.
  • the concentration of virus in the first hole is determined taking into account the degree of dilution. For the purpose of distributing the concentration of the virus, allocate the registration hole, in which a small amount of the depleted cell is left for a total of 0.5 cells.
  • the proposed device can be manufactured under the conditions of small business and industrial use, with the use of mining and quarrying. ' Sources of information: 0 1. ⁇ , ⁇ 2156325; 1972. 2. ⁇ , ° 2092378, 1972. 4. ⁇ , ⁇ 22001132198, 2003.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention relève du domaine de la biotechnologie et concerne en particulier un procédé permettant de déterminer la concentration de virus dans un matériau biologique liquide, lequel procédé consiste à présélectionner des cellules de test présentant une haute spécificité à l'égard d'un type de virus étudié; à déterminer la quantité moyenne de virus nécessaire pour infecter les cellules susmentionnées; à prélever des échantillons du matériau étudié sous forme liquide afin de détecter la présence de virus; à diluer ces échantillons selon un coefficient donné; à mélanger les échantillons dilués avec une suspension de cellules de test dans une cavité de contrôle selon une concentration donnée de cellules; à transférer le mélange d'échantillons étudiés de matériau comprenant une concentration donnée de cellules dans une chambre de mesure munie d'électrodes; à former un champ électrique alternatif dans la chambre entre les électrodes puis à déterminer la concentration de virus. Avant la formation du champ électrique alternatif dans la chambre de mesure, il convient de calculer la quantité totale de cellules présentes dans la zone d'observation au microscope sélectionnée entre les électrodes de cette chambre après avoir exposé la suspension de cellules à l'action du champ électrique alternatif pendant une durée suffisante pour qu'on observe la lyse de cellules intactes, de calculer le nombre de cellules restant dans la zone d'observation au microscope sélectionnée entre les électrodes de la chambre de mesure, puis de déterminer la part de cellules résistant au champ électrique alternatif et la concentration de virus dans le matériau biologique liquide sur la base de formules de calcul.
PCT/RU2004/000003 2004-01-09 2004-01-09 Procede permettant de determiner la concentration de virus dans un materiau biologique liquide WO2005066358A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/RU2004/000003 WO2005066358A1 (fr) 2004-01-09 2004-01-09 Procede permettant de determiner la concentration de virus dans un materiau biologique liquide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/RU2004/000003 WO2005066358A1 (fr) 2004-01-09 2004-01-09 Procede permettant de determiner la concentration de virus dans un materiau biologique liquide

Publications (1)

Publication Number Publication Date
WO2005066358A1 true WO2005066358A1 (fr) 2005-07-21

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Application Number Title Priority Date Filing Date
PCT/RU2004/000003 WO2005066358A1 (fr) 2004-01-09 2004-01-09 Procede permettant de determiner la concentration de virus dans un materiau biologique liquide

Country Status (1)

Country Link
WO (1) WO2005066358A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2156325A1 (fr) * 1971-10-14 1973-05-25 Coulter W
SU1394708A1 (ru) * 1986-04-15 1991-12-30 Институт биохимии им.А.Н.Баха Способ количественного определени вирусов
RU2001132198A (ru) * 2001-11-28 2003-07-10 Государственный научный центр вирусологии и биотехнологии "Вектор" Способ определения концентрации вирусов в жидком биологическом материале и устройство для его осуществления

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2225446C2 (ru) * 2001-11-28 2004-03-10 Государственный научный центр вирусологии и биотехнологии "Вектор" Способ определения концентрации вирусов в жидком биологическом материале и устройство для его осуществления

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2156325A1 (fr) * 1971-10-14 1973-05-25 Coulter W
SU1394708A1 (ru) * 1986-04-15 1991-12-30 Институт биохимии им.А.Н.Баха Способ количественного определени вирусов
RU2001132198A (ru) * 2001-11-28 2003-07-10 Государственный научный центр вирусологии и биотехнологии "Вектор" Способ определения концентрации вирусов в жидком биологическом материале и устройство для его осуществления

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