WO2005056765A3 - Methods for culturing keratinocytes from human embryonic stem cells - Google Patents

Methods for culturing keratinocytes from human embryonic stem cells Download PDF

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Publication number
WO2005056765A3
WO2005056765A3 PCT/US2004/040977 US2004040977W WO2005056765A3 WO 2005056765 A3 WO2005056765 A3 WO 2005056765A3 US 2004040977 W US2004040977 W US 2004040977W WO 2005056765 A3 WO2005056765 A3 WO 2005056765A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
stem cells
embryonic stem
human embryonic
culturing keratinocytes
Prior art date
Application number
PCT/US2004/040977
Other languages
French (fr)
Other versions
WO2005056765A2 (en
WO2005056765A8 (en
Inventor
Howard Green
Shiro Iuchi
Karen Easley
Original Assignee
Harvard College
Howard Green
Shiro Iuchi
Karen Easley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Howard Green, Shiro Iuchi, Karen Easley filed Critical Harvard College
Priority to AU2004296383A priority Critical patent/AU2004296383A1/en
Priority to EP04813310A priority patent/EP1706483A4/en
Priority to US10/582,028 priority patent/US20070274963A1/en
Publication of WO2005056765A2 publication Critical patent/WO2005056765A2/en
Publication of WO2005056765A3 publication Critical patent/WO2005056765A3/en
Publication of WO2005056765A8 publication Critical patent/WO2005056765A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to methods for isolating and culturing human keratinocytes from embryonic stem cells. The methods are useful for producing substantially pure cultures of keratinocytes.
PCT/US2004/040977 2003-12-08 2004-12-03 Methods for culturing keratinocytes from human embryonic stem cells WO2005056765A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2004296383A AU2004296383A1 (en) 2003-12-08 2004-12-03 Methods for culturing keratinocytes from human embryonic stem cells
EP04813310A EP1706483A4 (en) 2003-12-08 2004-12-03 Methods for culturing keratinocytes from human embryonic stem cells
US10/582,028 US20070274963A1 (en) 2003-12-08 2004-12-08 Methods for Culturing Keratinocytes from Human Embryonic Stem Cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52792003P 2003-12-08 2003-12-08
US60/527,920 2003-12-08

Publications (3)

Publication Number Publication Date
WO2005056765A2 WO2005056765A2 (en) 2005-06-23
WO2005056765A3 true WO2005056765A3 (en) 2005-09-09
WO2005056765A8 WO2005056765A8 (en) 2007-12-27

Family

ID=34676793

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/040977 WO2005056765A2 (en) 2003-12-08 2004-12-03 Methods for culturing keratinocytes from human embryonic stem cells

Country Status (3)

Country Link
EP (1) EP1706483A4 (en)
AU (1) AU2004296383A1 (en)
WO (1) WO2005056765A2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2825370A1 (en) * 2001-05-31 2002-12-06 Inst Nat Sante Rech Med KERATINOCYTES OBTAINED FROM MAMMALIAN EMBRYONIC STEM CELLS

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BAGUTTI C. ET AL: "Differentation of embryonal stem cells into keratinocytes: Comparison of wild-type and beta-1 integrin-deficient cells.", DEVELOPMENTAL BIOLOGY., vol. 179, 10 October 1996 (1996-10-10), pages 184 - 196, XP002190780 *
See also references of EP1706483A4 *

Also Published As

Publication number Publication date
EP1706483A4 (en) 2007-02-28
EP1706483A2 (en) 2006-10-04
AU2004296383A1 (en) 2005-06-23
WO2005056765A2 (en) 2005-06-23
WO2005056765A8 (en) 2007-12-27

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