WO2005040377A3 - High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences - Google Patents

High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences Download PDF

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Publication number
WO2005040377A3
WO2005040377A3 PCT/EP2004/011974 EP2004011974W WO2005040377A3 WO 2005040377 A3 WO2005040377 A3 WO 2005040377A3 EP 2004011974 W EP2004011974 W EP 2004011974W WO 2005040377 A3 WO2005040377 A3 WO 2005040377A3
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WO
WIPO (PCT)
Prior art keywords
expression
matrix attachment
high efficiency
mammalian cells
gene transfer
Prior art date
Application number
PCT/EP2004/011974
Other languages
French (fr)
Other versions
WO2005040377A2 (en
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
Original Assignee
Selexis Sa
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP04790766A priority Critical patent/EP1675952A2/en
Priority to AU2004284220A priority patent/AU2004284220B2/en
Priority to CA2535836A priority patent/CA2535836C/en
Priority to JP2006536060A priority patent/JP5396653B2/en
Priority to CN2004800292606A priority patent/CN1863913B/en
Priority to IN2483DEN2014 priority patent/IN2014DN02483A/en
Priority to IN2484DEN2014 priority patent/IN2014DN02484A/en
Priority to US10/595,495 priority patent/US8252917B2/en
Priority to KR1020117023131A priority patent/KR101230838B1/en
Priority to KR1020117023134A priority patent/KR101269656B1/en
Application filed by Selexis Sa, Nicolas Mermod, Pierre Alain Girod, Philipp Bucher, Duc-Quang Nguyen, David Calabrese, Damien Saugy, Stefania Puttini filed Critical Selexis Sa
Priority to KR1020067006395A priority patent/KR101222025B1/en
Priority to KR1020117023132A priority patent/KR101229989B1/en
Publication of WO2005040377A2 publication Critical patent/WO2005040377A2/en
Publication of WO2005040377A3 publication Critical patent/WO2005040377A3/en
Priority to IL175142A priority patent/IL175142A0/en
Priority to IL198863A priority patent/IL198863A/en
Priority to AU2010236091A priority patent/AU2010236091B2/en
Priority to AU2010236072A priority patent/AU2010236072B2/en
Priority to AU2010236093A priority patent/AU2010236093B2/en
Priority to US13/536,383 priority patent/US9879297B2/en
Priority to US15/846,279 priority patent/US10669562B2/en

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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.
PCT/EP2004/011974 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences WO2005040377A2 (en)

Priority Applications (19)

Application Number Priority Date Filing Date Title
KR1020067006395A KR101222025B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
AU2004284220A AU2004284220B2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
JP2006536060A JP5396653B2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by multiple transfection procedures of MAR sequences
CN2004800292606A CN1863913B (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
IN2483DEN2014 IN2014DN02483A (en) 2003-10-24 2004-10-22
IN2484DEN2014 IN2014DN02484A (en) 2003-10-24 2004-10-22
US10/595,495 US8252917B2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
KR1020117023132A KR101229989B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
KR1020117023134A KR101269656B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
EP04790766A EP1675952A2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
CA2535836A CA2535836C (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of mar sequences
KR1020117023131A KR101230838B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
IL175142A IL175142A0 (en) 2003-10-24 2006-04-24 High efficiency gene transfer and expresssion in mammalian cells by a multiple transfection procedure of mar sequences
IL198863A IL198863A (en) 2003-10-24 2009-05-21 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of mar sequences
AU2010236091A AU2010236091B2 (en) 2003-10-24 2010-10-27 High Efficiency Gene Transfer and Expression in Mammalian Cells by a Multiple Transfection Procedure of Matrix Attachment Region Sequence 27
AU2010236072A AU2010236072B2 (en) 2003-10-24 2010-10-27 High Efficiency Gene Transfer and Expression in Mammalian Cells by a Multiple Transfection Procedure of Matrix Attachment Region Sequence 26
AU2010236093A AU2010236093B2 (en) 2003-10-24 2010-10-27 High Efficiency Gene Transfer and Expression in Mammalian Cells by a Multiple Transfection Procedure of Matrix Attachment Region Sequence 24
US13/536,383 US9879297B2 (en) 2003-10-24 2012-06-28 High efficiency gene transfer and expression in mammalian cells by amultiple transfection procedure of MAR sequences
US15/846,279 US10669562B2 (en) 2003-10-24 2017-12-19 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
US60/513,574 2003-10-24
EP04002722 2004-02-06
EP04002722.9 2004-02-06

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10/595,495 A-371-Of-International US8252917B2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
US13/536,383 Continuation US9879297B2 (en) 2003-10-24 2012-06-28 High efficiency gene transfer and expression in mammalian cells by amultiple transfection procedure of MAR sequences
US13/536,383 Division US9879297B2 (en) 2003-10-24 2012-06-28 High efficiency gene transfer and expression in mammalian cells by amultiple transfection procedure of MAR sequences

Publications (2)

Publication Number Publication Date
WO2005040377A2 WO2005040377A2 (en) 2005-05-06
WO2005040377A3 true WO2005040377A3 (en) 2005-09-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/011974 WO2005040377A2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

Country Status (16)

Country Link
US (3) US8252917B2 (en)
EP (5) EP2298898B1 (en)
JP (1) JP5396653B2 (en)
KR (4) KR101222025B1 (en)
CN (1) CN1863913B (en)
AT (1) ATE551420T1 (en)
AU (4) AU2004284220B2 (en)
CA (1) CA2535836C (en)
HK (4) HK1151824A1 (en)
IL (2) IL175142A0 (en)
IN (2) IN2014DN02483A (en)
PL (4) PL2298898T3 (en)
RU (1) RU2375450C2 (en)
SG (1) SG147468A1 (en)
WO (1) WO2005040377A2 (en)
ZA (1) ZA200604032B (en)

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