WO2005037052A2 - Binding assay with labelled bispidine variant - Google Patents
Binding assay with labelled bispidine variant Download PDFInfo
- Publication number
- WO2005037052A2 WO2005037052A2 PCT/SE2004/001499 SE2004001499W WO2005037052A2 WO 2005037052 A2 WO2005037052 A2 WO 2005037052A2 SE 2004001499 W SE2004001499 W SE 2004001499W WO 2005037052 A2 WO2005037052 A2 WO 2005037052A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- compound
- assay
- formula
- channel
- bis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/08—Bridged systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/06—Antiarrhythmics
Definitions
- the present invention relates to a labelled ligand compound that is useful in a method of identifying, testing and/or screening of compounds modulating ion channels, in particular myocardial I ⁇ r channels such as those encoded by ERG, including hERG.
- the ligand compound is therefore of use to evaluate the affinity of preclinical compounds at the ERG potassium channel.
- Torsades de Pointes (Vandenberg JI, Walker BD, Campbell TJ. HERG K + channels: friend and foe. Trends Pharmacol Sci 2001; 22(5): 240-6).
- hERG myocardial I r channel
- Patients with LQT 2 syndrome, in which the human ether- a-go-go (hERG) gene is mutated also exhibit Torsades de Pointes.
- the hERG channel contains a binding site, for example, for the class III antiarrhythmic methanesulphonanilides (dofetilide, E-4031 and MK-449) and many drugs that cause prolonged QT in the clinic share such a site. Consequently they have either warning labels (e.g. pimozide) or have been withdrawn from the market (e.g. terfenadine).
- warning labels e.g. pimozide
- the lack of an appropriate therapeutic margin between activity at the hERG channel and the desired target will prevent a potential drug from progressing further. It is therefore necessary to evaluate the hERG activity as early as possible to allow reduction in this activity for novel compound classes.
- WO 02/04446 discloses bispidine compounds and their use in the treatment of cardiac arrhythmias.
- the present invention relates to a radiolabelled derivative of a bispidine compound as described in WO 02/04446 or salts, hydrates or solvates thereof.
- said compound comprises radiolabelled substitutions and, in particular, tritium substitutions.
- the compound comprises at least 1, 2 or 3 tritium substitutions.
- the invention relates to a compound having Formula I or salts, hydrates or solvates thereof and comprising at least one radiolabel: FORMULA I
- said compound comprises at least 1, 2 or 3 tritium substitutions preferably ortho to the nitro group.
- radioligands or radiolabelled compounds are useful to evaluate affinity of compounds at the I ⁇ r channel encoded by ERG, and in particular hERG.
- Suitable salts include acid addition or base salts thereof.
- a review of some suitable salts may be found in Berge et al, J Pharm Sci, 66, 1-19 (1977). Salts are formed, for example with strong inorganic acids such as mineral acids, e.g.
- organic carboxylic acids such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted (e.g., by halogen), such as acetic acid
- saturated or unsaturated dicarboxylic acids for example oxalic, malonic, succinic, maleic, fumaric, phthalic or terephthalic
- hydroxycarboxylic acids for example ascorbic, glycolic, lactic, malic, tartaric or citric acid
- aminoacids for example aspartic or glutamic acid
- benzoic acid or with organic sulfonic acids, such as (C 1 -C 4 )-alkyl- or aryl-sulfonic acids which are unsubstituted or substituted (for example, by a halogen) such as methane- or p-toluene sulfonic acid.
- the invention also includes all enantiomers and tautomers of the radiolabelled compound of Formula I or II.
- the person skilled in the art will recognise compounds that possess optical properties (one or more chiral carbon atoms) or tautomeric characteristics.
- the corresponding enantiomers and/or tautomers may be isolated/prepared by methods known in the art.
- the invention includes any stereoisomers and/or geometric isomers of the radiolabelled compound of Formula I or II.
- the present invention contemplates the use of all the individual stereoisomers and geometric isomers of those agents, and mixtures thereof.
- the terms used in the claims encompass these forms, provided said forms retain the appropriate functional activity (though not necessarily to the same degree).
- the present invention also includes other isotopic variations of the compound or its salt.
- An isotopic variation of an agent of the present invention or a pharmaceutically acceptable salt thereof is defined as one in which at least one atom is replaced by an atom having the same atomic number but an atomic mass different from the atomic mass most commonly found in nature.
- further isotopes that can be incorporated into the compound include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulphur, fluorine and chlorine such as 2 H, 1H, 13 C, 14 C, 15 N, 17 0, 18 0, 3, P, 32 P, 35 S, 18 F and 36 C1, respectively.
- the tritiated form, i.e., 3 H is particularly preferred.
- Carbon-14, i.e., 14 C, isotopes may also be used and are preferred for their ease of preparation and detectability.
- Isotopic variations can generally be prepared by conventional procedures using appropriate isotopic variations of suitable reagents.
- the present invention also includes the use of solvate forms of the compound.
- the terms used in the claims encompass these forms.
- a radioligand compound in a binding assay relies on a number of parameters including the affinity of the radioligand for the channel of interest as well as the off rate of the compound once it has bound.
- a suitable radioligand will have an affinity that allows competition with a candidate compound which can bind the same site as well as an off rate that allows the radioligand to remain in contact long enough for its binding to be detectable. These properties in a compound cannot be predicted.
- the present invention relates to the finding that a 1, 2 or 3 tritium substituted compound of Formula I or a compound of Formula II can be synthesised and is suitable for use in a competitive binding assay.
- said method is an assay comprising the following steps: a) incubation of cell membrane containing the I ⁇ r channel in the presence of the radioligand compound of Formula II in the presence or absence of a test compound or a mixture of test compounds; b) quantitation of specifically bound labelled compound in the presence or absence of a test compound; c) calculation of the inhibition of labelled compound binding by the test compound or mixture of test compounds.
- Such an assay is useful for the identification and characterisation of compounds that have potentially cardiotoxic side effects.
- the assay measures the ability of the test compound to displace the radioligand compound of Formula II from the Ikr channel which is, preferably, the ERG channel.
- the assay can be performed in a high throughput test system.
- the assay enables suitable compounds (i.e. those having no, low or reduced affinity to the Ikr channel) to be selected for further development..
- such an assay is useful in identifying compounds that are capable of binding the I r channel and therefore potentially useful in the treatment of patients with arrythmias.
- said assay comprises the steps of : a) preparing solutions of test compound at one or more concentrations; b) mixing the radioligand compound of Formula II with the cell membrane containing the I r channel; c) incubating the solutions of test compound with the mixture of radioligand compound of Formula II and cell membrane containing the I ⁇ r channel; d) isolating the membrane from the solutions and measuring the radioactivity of the membrane; e) calculating the radioactivity of samples in the presence of test compound compared to a control in the absence of test compound.
- the concentration of the test compound that gives 50% inhibition of binding of the radioligand compound to the cell membrane (IC 5 o) is calculated. These values can be used to predict the concentration of the compound liable to cause undesirable side effects in humans.
- the assay is a spot test. In another embodiment, the assay tests a range of concentrations and, preferably, five or more concentrations.
- Suitable methods for isolating the membrane and measuring radioactivity include filter binding assays such as the assay described herein.
- radioactivity may be measured using a bead based assay such as a scintillation proximity assay (SPA, Amersham Biosciences).
- SPA scintillation proximity assay
- the I ⁇ r channel is human ERG (hERG/HERG).
- the cell membrane comprising the I r channel is derived from a cell line transfected with the ERG gene.
- the ERG gene is human (hERG), primate or canine.
- HERG has been expressed as stable or transiently expressed functional channels in human embryonic kidney (HEK) cells (see, for example, Circulation 2001 Nov 27;104(22):2645-8 Phospholipid metabolite 1-palmitoyl-lysophosphatidylcholine enhances human ether-a-go- go-related gene (HERG) K(+) channel function.
- HEK human embryonic kidney
- suitable cell lines include HEK (human embryonic kidney) cells such as HEK 293 cells.
- Other suitable cell lines include CHO (chinese hamster ovary), CHL (chinese hamster lung) or COS (monkey) cells.
- the channel may be expressed in bacterial, yeast or insect cells such as SF9.
- the assay is a competitive binding assay.
- a process for preparing a compound of Formula II as defined herein comprising tritiating 3,7-Bis[2-(4- nitrophenyl)ethyl]-3,7-diazabicyclo[3.3.1]nonane in the presence of (1,5- cyclooctadiene)bis(methyldiphenyl-phosphine)iridium(I) hexafluorophosphate.
- the 3,7-Bis[2-(4-nitrophenyl)ethyl]-3,7-diazabicyclo[3.3.1]nonane and (1,5- cyclooctadiene)bis(methyldiphenyl-phosphine)iridium(I) hexafluorophosphate are dissolved in dichloromethane.
- tritiation is carried out using a tritiation manifold.
- the process for preparing a compound of Formula II is substantially as described herein.
- the dichloromethane solution is finally frozen in liquid nitrogen and tritium gas (approximately 130GBq, 61 ⁇ mol), generated from the primary uranium bed on the manifold, is introduced into the void above the solution.
- tritium gas approximately 130GBq, 61 ⁇ mol
- the reaction is allowed to warm to room temperature and stirred for 20 hours, via means of a magnetic stirrer bar.
- the solution is refrozen in liquid nitrogen and residual tritium gas reabsorbed onto the secondary uranium storage bed of the manifold.
- the flask is removed from the manifold and the solution subjected to two cycles of lyophilisation using ethanol (2 x 5ml) to remove labile tritium.
- the resulting residue is dissolved in ethanol (10ml) to afford the stock solution of 3,7-Bis[2-(4-nitro[3,5- 3 H]phenyl)ethyl]-3,7-diazabicyclo[3.3. ljnonane (19.33 GBq, radiochemical purity 34%).
- ljnonane (retention time 10.77min) is collected each time and combined (radiochemical purity >97%).
- Aqueous sodium thiosulphate (50% w/v, 50 ⁇ l) is added to the combined fractions to stabilise the sample prior to removal of the organic solvent under reduced pressure.
- the resulting aqueous solution is freeze-dried to leave a white residue which is reconstituted in ethanol water, 2:1 by volume (15ml) to afford the purified stock of 3,7-Bis[2-(4-nitro[3,5- 3 H]phenyl)ethyl]- 3,7-diazabicyclo[3.3.1]nonane (881.7MBq; radiochemical purity 97.5%).
- the molar specific radioactivity is determined by mass spectrometry and is found to be 2941.5 GBq mmol "1 .
- GF/B filter plates are pre-soaked in coating solution for a minimum of 20 minutes before harvesting.
- Membranes for use in the assay are prepared as follows:
- HEK (human embryonic kidney) cells are grown using standard methods to give the required number, they are then harvested and pelleted. The final pellet is prepared in serum-free medium and the packed cell volume (pcv) measured. The pellet is resuspended in 4 times the pcv in hypotonic buffer solution (3 parts water: 1 part serum free medium or buffer) with 1 tablet / 50ml of Boehringer protease inhibitor ( Cat No 1 697 498 ) added.
- the cells are allowed to swell for a couple of minutes on ice then lysed using a Polytron tissue homogeniser set to 22,000 rpm.
- the cell suspension is held on ice and the Polytron is allowed to build up to speed, then turned off and allowed to cool for 30 seconds before repeating. Normally 3 bursts should be sufficient to completely lyse the cells.
- a sample is checked microscopically to ensure complete lysis and the process repeated if necessary.
- Membranes are seen as a white band at sucrose / buffer interphase. The top layer of the buffer is discarded and then all the membrane band is harvested with as little sucrose as possible and added to a fresh centrifuge tube. This is diluted and mixed well with at least 3 volumes of ice cold buffer containing protease inhibitor. Centrifugation is carried out at 23,000 rpm at 4°C for 20 minutes with a break to zero.
- the membrane solution is diluted 65-fold, e.g. 0.5 mL membranes diluted to 32.5 mL assay buffer per assay plate.
- Total binding of 3,7-Bis[2-(4-nitro[3,5- 3 H]phenyl)ethyl]-3,7-diazabicyclo[3.3.1]nonane does not exceed 10% of the total added.
- 3,7-Bis[2-(4-nitro[3,5- 3 H]phenyl)ethyl]-3,7-diazabicyclo[3.3.1]nonane is made according to the above protocol and supplied in 67% (v/v) ethanol, 33% (v/v) water containing 3.3 mg/mL sodium thiosulphate.
- the batch of radio label used in these studies has a specific activity of 2941.5 GBq/mmol (79.5 Ci/mmol) and a concentration of 20 ⁇ M.
- the stock radiolabel is diluted 2000-fold to 10 nM and 20 ⁇ L added per well to a final assay volume of 200 ⁇ L to give a final radioligand concentration of 1 nM.
- Total binding Total binding, in the absence of a competing ligand, is defined in the presence of a vehicle control. In this case it was 1% (v/v) DMSO (20 ⁇ L per well from a stock of 10% (v/v) DMSO in assay buffer).
- NSB Non-specific binding is determined by measuring the binding of 3,7-Bis[2-(4-nitro[3,5- 3 H]phenyl)ethyl]-3,7-diazabicyclo[3.3.1]nonane in the presence of 10 ⁇ M astemizole (20 ⁇ L per well from a stock of 100 ⁇ M in 10% (v/v) DMSO in assay buffer).
- Test compounds are dissolved in DMSO to a stock concentration of 10 mM. Typically up to 7 compounds are tested per plate, with an internal standard. These are diluted simultaneously in a 96-well plate using an 8-channel pipette. Compounds are serially diluted in DMSO to lOOx the final assay concentrations to be used, typically half log units from 3 mM to 30 ⁇ M. This is achieved by adding 30 ⁇ L of each successive dilution to 65 ⁇ L DMSO in a 96-well plate. Further dilutions are carried out in assay buffer as follows: compounds are diluted 10-fold with assay buffer to give 5 concentrations of compound in 10% DMSO. 20 ⁇ L of each of these dilutions is then added to the assay plate. When the other additions are made this makes a total volume of 200 ⁇ L and a final DMSO concentration of 1% (v/v). ASSAY STANDARD
- Pimozide a hERG-active compound
- the compound is made up as 10 mM stock in DMSO and frozen in aliquots at -20°C for use in each experiment. One standard compound is tested in each assay plate.
- Assays are performed in Costar polypropylene round-bottomed 96 well plates. Each assay plate contains controls for total binding and non-specific binding. Compounds are usually tested at five log step dilutions, in duplicate. This allows eight compounds to be tested per assay plate. Typically compounds are tested over the range 30 ⁇ M to 0.3 ⁇ M. Additions to each assay plate are summarised in Table 1 :
- the plates are placed on a plate shaker for 1 minute to allow mixing. The plates are then incubated for 3 hours at room temperature (18-20°C). During this time one GF/B plate for each assay plate is immersed in coating solution. After incubation the assay well contents are harvested using a Tomtec harvester and washed seven times with approximately 250 ⁇ L wash buffer per cycle (1750 ⁇ L total). The plate layout is transposed in the harvester so that well Al in the assay plate is harvested onto well A12 in the GF/B plate.
- the mean CPM values for total binding, non-specific binding and total [ 3 H] added are calculated.
- the mean CPM value is also calculated for each concentration of test compound and the mean NSB subtracted.
- a concentration-effect curve for each test compound is constructed by plotting the mean CPM against the concentration of test compound. The pIC 50 value is then determined.
- a range of compounds representing a diverse range of structural classes are screened in the 3,7-Bis[2-(4-nitro[3, 5- 3 HJphenyl)ethylJ-3,7-diazabicyclo[3.3.1 Jnonane binding assay.
- Comparison of these data with functional pIC 5 o data from the electrophysiology studies shows that the majority of compounds were 3 to 10-fold less active in the binding assay than in electrophysiology. However, all compounds with functional activity of greater than 10 micromolar were detected in the binding assay.
- the data for the standard compounds indicates that the binding assay would be appropriate for investigating SAR of channel blockers. In contrast to the standards, when dealing with much less potent compounds as is usual for project compounds, clear SAR is not within the sensitivity of the assay.
- the binding assay can be used to detect compound classes with potential to cause QT prolongation.
- This binding assay is suitable for use as a primary hERG screen, minimising the number of compounds that need to be tested by electrophysiology.
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Cardiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Heart & Thoracic Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Materials For Medical Uses (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/575,523 US20070048215A1 (en) | 2003-10-20 | 2004-10-18 | Chemical compound and assay |
JP2006536480A JP2007513874A (en) | 2003-10-20 | 2004-10-18 | Binding assays with labeled bispidine variants |
DE602004014633T DE602004014633D1 (en) | 2003-10-20 | 2004-10-18 | CHEMICAL CONNECTION AND TEST |
EP04793802A EP1678499B1 (en) | 2003-10-20 | 2004-10-18 | Chemical compound and assay |
HK06109478A HK1089233A1 (en) | 2003-10-20 | 2006-08-25 | Chemical compound and assay |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0302775-2 | 2003-10-20 | ||
SE0302775A SE0302775D0 (en) | 2003-10-20 | 2003-10-20 | Chemical compound and assay |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005037052A2 true WO2005037052A2 (en) | 2005-04-28 |
WO2005037052A3 WO2005037052A3 (en) | 2005-07-07 |
Family
ID=29546593
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SE2004/001499 WO2005037052A2 (en) | 2003-10-20 | 2004-10-18 | Binding assay with labelled bispidine variant |
Country Status (11)
Country | Link |
---|---|
US (1) | US20070048215A1 (en) |
EP (1) | EP1678499B1 (en) |
JP (1) | JP2007513874A (en) |
CN (1) | CN1871516A (en) |
AT (1) | ATE399322T1 (en) |
DE (1) | DE602004014633D1 (en) |
ES (1) | ES2307058T3 (en) |
HK (1) | HK1089233A1 (en) |
SE (1) | SE0302775D0 (en) |
TW (1) | TW200526255A (en) |
WO (1) | WO2005037052A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007011293A1 (en) | 2005-07-21 | 2007-01-25 | Astrazeneca Ab | Novel piperidine derivatives |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2428792A1 (en) * | 1974-06-14 | 1976-01-02 | Knoll Ag | NEW ANTIARRHYTHMICS |
US4550112A (en) * | 1982-09-18 | 1985-10-29 | Kali-Chemie Pharma Gmbh | 3,7-Diazabicyclo(3,3,1)nonane compounds and their use in treating heart disease |
DE3730224A1 (en) * | 1987-09-09 | 1989-03-23 | Kali Chemie Pharma Gmbh | 3-Cinnamyl-3,7-diazabicyclo[3.3.1]nonane compounds, process for their preparation and medicaments containing these compounds |
EP0308843A2 (en) * | 1987-09-24 | 1989-03-29 | BASF Aktiengesellschaft | Bispidin derivatives as class III antiarrhythmics |
WO2002004446A1 (en) * | 2000-07-07 | 2002-01-17 | Astrazeneca Ab | New bispidine compounds and their use in the treatment of cardiac arrhythmias |
WO2002005860A1 (en) * | 2000-07-14 | 2002-01-24 | Merck & Co., Inc. | Radioligand and binding assay |
WO2003006988A1 (en) * | 2001-07-13 | 2003-01-23 | Janssen Pharmaceutica N.V. | Cardiovascular safety assay |
WO2003021271A2 (en) * | 2001-09-04 | 2003-03-13 | Pfizer Limited | Affinity-assay for the human erg potassium channel |
-
2003
- 2003-10-20 SE SE0302775A patent/SE0302775D0/en unknown
-
2004
- 2004-10-12 TW TW093130894A patent/TW200526255A/en unknown
- 2004-10-18 DE DE602004014633T patent/DE602004014633D1/en not_active Expired - Fee Related
- 2004-10-18 US US10/575,523 patent/US20070048215A1/en not_active Abandoned
- 2004-10-18 ES ES04793802T patent/ES2307058T3/en active Active
- 2004-10-18 EP EP04793802A patent/EP1678499B1/en not_active Not-in-force
- 2004-10-18 JP JP2006536480A patent/JP2007513874A/en active Pending
- 2004-10-18 AT AT04793802T patent/ATE399322T1/en not_active IP Right Cessation
- 2004-10-18 CN CNA2004800307531A patent/CN1871516A/en active Pending
- 2004-10-18 WO PCT/SE2004/001499 patent/WO2005037052A2/en active Application Filing
-
2006
- 2006-08-25 HK HK06109478A patent/HK1089233A1/en not_active IP Right Cessation
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2428792A1 (en) * | 1974-06-14 | 1976-01-02 | Knoll Ag | NEW ANTIARRHYTHMICS |
US4550112A (en) * | 1982-09-18 | 1985-10-29 | Kali-Chemie Pharma Gmbh | 3,7-Diazabicyclo(3,3,1)nonane compounds and their use in treating heart disease |
DE3730224A1 (en) * | 1987-09-09 | 1989-03-23 | Kali Chemie Pharma Gmbh | 3-Cinnamyl-3,7-diazabicyclo[3.3.1]nonane compounds, process for their preparation and medicaments containing these compounds |
EP0308843A2 (en) * | 1987-09-24 | 1989-03-29 | BASF Aktiengesellschaft | Bispidin derivatives as class III antiarrhythmics |
WO2002004446A1 (en) * | 2000-07-07 | 2002-01-17 | Astrazeneca Ab | New bispidine compounds and their use in the treatment of cardiac arrhythmias |
WO2002005860A1 (en) * | 2000-07-14 | 2002-01-24 | Merck & Co., Inc. | Radioligand and binding assay |
WO2003006988A1 (en) * | 2001-07-13 | 2003-01-23 | Janssen Pharmaceutica N.V. | Cardiovascular safety assay |
WO2003021271A2 (en) * | 2001-09-04 | 2003-03-13 | Pfizer Limited | Affinity-assay for the human erg potassium channel |
Non-Patent Citations (2)
Title |
---|
CHIAVARELLI S. ET AL: 'Sintesi nella serie dell' 1,5-difenilbispidin-9-ONE' IL FARMACO vol. 20, no. 6, 1965, pages 108 - 420, XP002986105 * |
MANOTTI LANFREDI A.M. ET AL: 'Iridium(III) cis-Dihydrido complexes with 3,6-Bis(2'-pyridyl)pyrizidane. Chrystal Structure of the Pyridazinyl Complex ((IrH2(PPh3)2(u-C4HN2(C5H4N)2-3,6))PF6' J CHEM SOC DALTON TRANS no. 1, 1988, pages 651 - 656, XP002986104 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007011293A1 (en) | 2005-07-21 | 2007-01-25 | Astrazeneca Ab | Novel piperidine derivatives |
EP2402316A1 (en) | 2005-07-21 | 2012-01-04 | AstraZeneca AB (Publ) | Piperidine derivatives |
Also Published As
Publication number | Publication date |
---|---|
EP1678499B1 (en) | 2008-06-25 |
TW200526255A (en) | 2005-08-16 |
ES2307058T3 (en) | 2008-11-16 |
SE0302775D0 (en) | 2003-10-20 |
CN1871516A (en) | 2006-11-29 |
EP1678499A2 (en) | 2006-07-12 |
DE602004014633D1 (en) | 2008-08-07 |
WO2005037052A3 (en) | 2005-07-07 |
ATE399322T1 (en) | 2008-07-15 |
HK1089233A1 (en) | 2006-11-24 |
US20070048215A1 (en) | 2007-03-01 |
JP2007513874A (en) | 2007-05-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101707764B1 (en) | Apoptosis signal-regulating kinase inhibitor | |
Priest et al. | Role of hERG potassium channel assays in drug development | |
Huang et al. | Identification of human Ether-à-go-go related gene modulators by three screening platforms in an academic drug-discovery setting | |
Moore | NMR techniques for characterization of ligand binding: utility for lead generation and optimization in drug discovery | |
KR101928225B1 (en) | N-(cyanomethyl)-4-(2-(4-morpholinophenylamino)pyrimidin-4-yl)benzamide hydrochloride salts | |
Fejzo et al. | Application of NMR screening in drug discovery | |
Lovering et al. | Rational approach to highly potent and selective apoptosis signal-regulating kinase 1 (ASK1) inhibitors | |
CN104788560A (en) | Cyclosporin A immunogen, anti-cyclosporin A specific antibody and cyclosporin A detection reagent | |
TW201219393A (en) | Compound useful as a c-Met inhibitor | |
JP6589246B2 (en) | SSAO inhibitor derived from imidazo [4,5-C] pyridine | |
EP1678499B1 (en) | Chemical compound and assay | |
JPWO2019009355A1 (en) | Drug for treating lipid peroxidation-induced disease and method for screening the same | |
Whitehurst et al. | Discovery and characterization of orthosteric and allosteric muscarinic M2 acetylcholine receptor ligands by affinity selection–mass spectrometry | |
JP6637034B2 (en) | Radiolabeled cannabinoid receptor 2 ligand | |
AU2011200195B2 (en) | High Throughput Assay Systems And Methods For Identifying Agents That Alter Surface Expressions Of Intergral Membrane Proteins | |
JP2002531831A (en) | New assay method | |
Orosz et al. | Functional in vitro test of calmodulin antagonism: effect of drugs on interaction between calmodulin and glycolytic enzymes. | |
US20030109536A1 (en) | (Oxo-pyrazolo[1,5a]pyrimidin-2-yl) alkyl-carboxamides | |
Appell et al. | Biological characterization of neurokinin antagonists discovered through screening of a combinatorial library | |
JP2007513874A5 (en) | ||
US20060201886A1 (en) | Method for determining the binding behavior of ligands which specifically bind to target molecules | |
Anana et al. | Synthesis and Smooth Muscle Calcium Channel Effects of Dialkyl 1, 4‐Dihydro‐2, 6‐dimethyl‐4‐aryl‐3, 5‐pyridinedicarboxylates Containing a Nitrone Moiety in the 4‐Aryl Substituent | |
CN1997892A (en) | Ligand analysis | |
Givant et al. | Quantitation of nitroglycerin in human blood after administration by sustained release | |
Schultz | Practical aspects of using NMR in fragment-based screening |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 200480030753.1 Country of ref document: CN |
|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2004793802 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2007048215 Country of ref document: US Ref document number: 10575523 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006536480 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2605/DELNP/2006 Country of ref document: IN |
|
WWP | Wipo information: published in national office |
Ref document number: 2004793802 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 10575523 Country of ref document: US |