WO2005028613A3 - Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications. - Google Patents

Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications. Download PDF

Info

Publication number
WO2005028613A3
WO2005028613A3 PCT/FR2004/002221 FR2004002221W WO2005028613A3 WO 2005028613 A3 WO2005028613 A3 WO 2005028613A3 FR 2004002221 W FR2004002221 W FR 2004002221W WO 2005028613 A3 WO2005028613 A3 WO 2005028613A3
Authority
WO
WIPO (PCT)
Prior art keywords
met4
protein
ubiquitine
hydrolases
modulate
Prior art date
Application number
PCT/FR2004/002221
Other languages
English (en)
Other versions
WO2005028613A2 (fr
Inventor
Dominique Thomas
Regine Barbey
Original Assignee
Centre Nat Rech Scient
Dominique Thomas
Regine Barbey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Dominique Thomas, Regine Barbey filed Critical Centre Nat Rech Scient
Publication of WO2005028613A2 publication Critical patent/WO2005028613A2/fr
Publication of WO2005028613A3 publication Critical patent/WO2005028613A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé de criblage cellulaire de composés aptes à moduler l'activité des ubiquitine hydrolases comprenant les étapes suivantes : la culture en milieu riche d'une souche de levure modifiée, comportant une séquence hybride comportant une séquence codant pour une protéine (Met4) et un système transcriptionnel rapporteur comprenant un promoteur de levure activé par le facteur transcriptionnel (Met4), le transfert de la culture dans un milieu riche et pendant une durée permettant l'expression de la protéine (Met4), le transfert de la culture dans un milieu ne permettant pas l'expression de la protéine (Met4), la mise en contact de la souche de levure ainsi cultivée avec un composé à tester, l'addition de cadmium, la détermination du niveau d'expression de la protéine hybride exprimée à partir d'un promoteur MET.
PCT/FR2004/002221 2003-09-15 2004-09-01 Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications. WO2005028613A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0310789A FR2859733A1 (fr) 2003-09-15 2003-09-15 Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications
FR0310789 2003-09-15

Publications (2)

Publication Number Publication Date
WO2005028613A2 WO2005028613A2 (fr) 2005-03-31
WO2005028613A3 true WO2005028613A3 (fr) 2005-06-16

Family

ID=34203434

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/002221 WO2005028613A2 (fr) 2003-09-15 2004-09-01 Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications.

Country Status (2)

Country Link
FR (1) FR2859733A1 (fr)
WO (1) WO2005028613A2 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997012962A1 (fr) * 1995-10-04 1997-04-10 Cold Spring Harbor Laboratory Ubiquitine ligases et utilisations associees
WO1999004033A1 (fr) * 1997-07-16 1999-01-28 Mitotix, Inc. ESSAI PERMETTANT DE DECELER LES INHIBITEURS DE LA PROTEOLYSE DU I KAPPA B (IλB)
WO1999018989A1 (fr) * 1997-10-16 1999-04-22 Baylor College Of Medicine Proteines et genes a sequence f
US5932425A (en) * 1997-02-18 1999-08-03 Signal Pharmaceuticals, Inc. Compositions and methods for modulating cellular NF-κB activation
WO1999038969A1 (fr) * 1998-01-30 1999-08-05 Institut National De La Sante Et De La Recherche Medicale PROTEINE HUMAINE β-TrCP
WO2001040499A2 (fr) * 1999-12-01 2001-06-07 Centre National De La Recherche Scientifique-Cnrs Procedes de criblage cellulaire de composes aptes a moduler l'activite des complexes ubiquitine-ligases scf et leurs applications
WO2002008394A2 (fr) * 2000-07-14 2002-01-31 Millenium Pharmaceuticals, Inc. 23430, nouveau membre de la famille des ubiquitines hydrolases de l'homme et ses utilisations

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997012962A1 (fr) * 1995-10-04 1997-04-10 Cold Spring Harbor Laboratory Ubiquitine ligases et utilisations associees
US5932425A (en) * 1997-02-18 1999-08-03 Signal Pharmaceuticals, Inc. Compositions and methods for modulating cellular NF-κB activation
WO1999004033A1 (fr) * 1997-07-16 1999-01-28 Mitotix, Inc. ESSAI PERMETTANT DE DECELER LES INHIBITEURS DE LA PROTEOLYSE DU I KAPPA B (IλB)
WO1999018989A1 (fr) * 1997-10-16 1999-04-22 Baylor College Of Medicine Proteines et genes a sequence f
WO1999038969A1 (fr) * 1998-01-30 1999-08-05 Institut National De La Sante Et De La Recherche Medicale PROTEINE HUMAINE β-TrCP
WO2001040499A2 (fr) * 1999-12-01 2001-06-07 Centre National De La Recherche Scientifique-Cnrs Procedes de criblage cellulaire de composes aptes a moduler l'activite des complexes ubiquitine-ligases scf et leurs applications
WO2002008394A2 (fr) * 2000-07-14 2002-01-31 Millenium Pharmaceuticals, Inc. 23430, nouveau membre de la famille des ubiquitines hydrolases de l'homme et ses utilisations

Also Published As

Publication number Publication date
WO2005028613A2 (fr) 2005-03-31
FR2859733A1 (fr) 2005-03-18

Similar Documents

Publication Publication Date Title
EP1824977A4 (fr) Nouvel acide nucleique de promoteur issu de la bacterie du genre corynebacterium, cassette d'expression contenant le promoteur et vecteur contenant la cassette, cellule hote comprenant le vecteur et procede d'expression d'un gene au moyen de la cellule
WO2003042384A1 (fr) Inducteur de differentiation pour cellules souches d'embryon, procede d'obtention dudit inducteur et utilisation
LT1680509T (lt) Angliavandenilių ir junginių, kurių sudėtyje yra deguonės, gavimo būdas iš biomasės, įskaitant fermentaciją su pridėtinėmis aminorūgštimis (leu, ile, val)
ATE397059T1 (de) Verfahren zur herstellung von l-lysin oder l- threonin unter verwendung von escherichia- bakterien mit abgeschwächter malatenzymaktivität
NZ595495A (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
AU3246300A (en) Polypeptides having galactose oxidase activity and nucleic acids encoding same
ATE450605T1 (de) Phytase enzyme, dafür kodierende nukleinsäuren, und solche enthaltende vectoren und wirtszellen
WO2006043178A3 (fr) Enzymes et procedes
WO2003044045A3 (fr) Peptides promouvant l'adherence, la croissance et la secretion des cellules
ATE419265T1 (de) Benzoat- und antranilatinduzierbare promotoren
AU2003238717A1 (en) POLYPEPTIDES HAVING Alpha- H-Alpha-AMINO ACID AMIDE RACEMASE ACTIVITY AND NUCLEIC ACIDS ENCODING THE SAME
EP1026249A4 (fr) GENE CODANT LA SOUS-UNITE alpha DE LA LIGASE D'ANTHRANILATE DU RIZ ET ADN S'Y RAPPORTANT
WO2003064630A3 (fr) Milieu de culture cellulaire
WO2002030958A3 (fr) Fragment peptidique de caseine presentant une activite influant sur la croissance de cultures cellulaires
EP1760152A4 (fr) Procédé servant à produire un dipeptide
WO2005060722A3 (fr) Modulation de la fonction du systeme immunitaire par la modulation des polypeptides arginine-methyltransferases
WO2006015802A3 (fr) Procede de production d'alcools primaires
WO2005097818A3 (fr) Systeme de dosage permettant de cribler des inhibiteurs de protease
WO2005028613A3 (fr) Procede de criblage fonctionnel d'agents aptes a moduler l'activite des ubiquitine hydrolases et leurs applications.
WO2003070873A3 (fr) Procede de culture de micro-organismes et bioreacteur
WO2007070390A3 (fr) Procede de prolongation de l'activite d'enzymes autodegradables
WO2005046581A3 (fr) Proteine p18 dans des manipulations de cellules souches
WO2003018792A1 (fr) Procede de detection d'une substance toxique
WO2005113750A3 (fr) Procedes et dispositifs pour mettre des cellules souches en culture
WO2004068139A3 (fr) Perfectionnement de l'efficacite catalytique et/ou de la specificite de substrats non naturels d'enzymes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BW BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE EG ES FI GB GD GE GM HR HU ID IL IN IS JP KE KG KP KZ LC LK LR LS LT LU LV MA MD MK MN MW MX MZ NA NI NO NZ PG PH PL PT RO RU SC SD SE SG SK SY TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SZ TZ UG ZM ZW AM AZ BY KG MD RU TJ TM AT BE BG CH CY DE DK EE ES FI FR GB GR HU IE IT MC NL PL PT RO SE SI SK TR BF CF CG CI CM GA GN GQ GW ML MR SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase