WO2005026369A3 - Interference rna expression vectors, methods for the production and use thereof - Google Patents

Interference rna expression vectors, methods for the production and use thereof Download PDF

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Publication number
WO2005026369A3
WO2005026369A3 PCT/FR2004/002267 FR2004002267W WO2005026369A3 WO 2005026369 A3 WO2005026369 A3 WO 2005026369A3 FR 2004002267 W FR2004002267 W FR 2004002267W WO 2005026369 A3 WO2005026369 A3 WO 2005026369A3
Authority
WO
WIPO (PCT)
Prior art keywords
interference rna
rna expression
production
methods
expression vectors
Prior art date
Application number
PCT/FR2004/002267
Other languages
French (fr)
Other versions
WO2005026369A2 (en
Inventor
Pascale Nony
Sophie Chappuis
Original Assignee
Transat
Pascale Nony
Sophie Chappuis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transat, Pascale Nony, Sophie Chappuis filed Critical Transat
Publication of WO2005026369A2 publication Critical patent/WO2005026369A2/en
Publication of WO2005026369A3 publication Critical patent/WO2005026369A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Abstract

The invention relates to an interference RNA expression vector characterised in that it comprises one or several insulating elements which protect DNA molecules encoding interference RNA against cell regulating sequences.
PCT/FR2004/002267 2003-09-08 2004-09-07 Interference rna expression vectors, methods for the production and use thereof WO2005026369A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0310565 2003-09-08
FR0310565A FR2859482B1 (en) 2003-09-08 2003-09-08 INTERFERENT RNA EXPRESSION VECTORS, PROCESSES FOR THEIR PREPARATION AND USES THEREOF

Publications (2)

Publication Number Publication Date
WO2005026369A2 WO2005026369A2 (en) 2005-03-24
WO2005026369A3 true WO2005026369A3 (en) 2005-06-09

Family

ID=34178841

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/002267 WO2005026369A2 (en) 2003-09-08 2004-09-07 Interference rna expression vectors, methods for the production and use thereof

Country Status (2)

Country Link
FR (1) FR2859482B1 (en)
WO (1) WO2005026369A2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5610053A (en) * 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AN D.S. ET AL.: "Efficient lentiviral vectors for short hairpin RNA delivery into human cells.", HUMAN GENE THERAPY, vol. 14, 10 August 2003 (2003-08-10), pages 1207 - 1212, XP002277891 *
RECILLAS-TARGA F ET AL: "Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activities", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 99, no. 10, 14 May 2002 (2002-05-14), pages 6883 - 6888, XP002274806, ISSN: 0027-8424 *
YANNAKI E., TUBB J., AKER M., STAMATOYANNOPOULOS G. & EMERY D.W.: "Topological constraints governing the use of chicken HS4 chromatin insulator in oncoretrogenic vecors.", MOLECULAR THERAPY, vol. 5, no. 5, May 2002 (2002-05-01), pages 589 - 598, XP002316215 *

Also Published As

Publication number Publication date
WO2005026369A2 (en) 2005-03-24
FR2859482B1 (en) 2005-11-11
FR2859482A1 (en) 2005-03-11

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