WO2005021712A3 - Modified molecular beacons - Google Patents

Modified molecular beacons Download PDF

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Publication number
WO2005021712A3
WO2005021712A3 PCT/US2004/020232 US2004020232W WO2005021712A3 WO 2005021712 A3 WO2005021712 A3 WO 2005021712A3 US 2004020232 W US2004020232 W US 2004020232W WO 2005021712 A3 WO2005021712 A3 WO 2005021712A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
molecular beacons
reporters
modified molecular
modified
Prior art date
Application number
PCT/US2004/020232
Other languages
French (fr)
Other versions
WO2005021712A2 (en
Inventor
Gang Bao
Nitin Nitin
Shuming Nie
Gloria J Kim
Original Assignee
Georgia Tech Res Inst
Gang Bao
Nitin Nitin
Shuming Nie
Gloria J Kim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georgia Tech Res Inst, Gang Bao, Nitin Nitin, Shuming Nie, Gloria J Kim filed Critical Georgia Tech Res Inst
Priority to CA002530221A priority Critical patent/CA2530221A1/en
Priority to JP2006517609A priority patent/JP2007525967A/en
Priority to EP04801961A priority patent/EP1639093A2/en
Publication of WO2005021712A2 publication Critical patent/WO2005021712A2/en
Publication of WO2005021712A3 publication Critical patent/WO2005021712A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0054—Macromolecular compounds, i.e. oligomers, polymers, dendrimers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/006—Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Nucleic acid reporters and methods of their use are provided. The nucleic acid reporters include molecular beacons modified with protein transduction domains to facilitate translocation of the nucleic acid reporter across cellular membranes. The nucleic acid reporters are also optionally modified with a targeting signal to direct the nucleic acid reporter to a specific cell, tissue, organ, intracellular region, organelle or vesicle.
PCT/US2004/020232 2003-06-25 2004-06-25 Modified molecular beacons WO2005021712A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002530221A CA2530221A1 (en) 2003-06-25 2004-06-25 Modified molecular beacons
JP2006517609A JP2007525967A (en) 2003-06-25 2004-06-25 Modified molecular beacon
EP04801961A EP1639093A2 (en) 2003-06-25 2004-06-25 Modified molecular beacons

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48264803P 2003-06-25 2003-06-25
US60/482,648 2003-06-25
US87492004A 2004-06-23 2004-06-23
US10/874,920 2004-06-23

Publications (2)

Publication Number Publication Date
WO2005021712A2 WO2005021712A2 (en) 2005-03-10
WO2005021712A3 true WO2005021712A3 (en) 2009-04-09

Family

ID=34278375

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/020232 WO2005021712A2 (en) 2003-06-25 2004-06-25 Modified molecular beacons

Country Status (4)

Country Link
EP (1) EP1639093A2 (en)
JP (1) JP2007525967A (en)
CA (1) CA2530221A1 (en)
WO (1) WO2005021712A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008098248A2 (en) * 2007-02-09 2008-08-14 Northwestern University Particles for detecting intracellular targets
US8586387B2 (en) * 2011-08-30 2013-11-19 Supernova Diagnostics, Inc. Methods of triggering activation of encapsulated signal-generating substances and apparatus utilising activated signal-generating substances
DE102012022596B4 (en) * 2012-11-15 2017-05-04 Friedrich-Schiller-Universität Jena New cell-specific effective nucleotide molecules and application kit for their application

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997023607A2 (en) * 1995-12-21 1997-07-03 The University Court Of The University Of Dundee Presentation of antigen, introduced into cells by stimulation of macropinocytosis
WO1999005302A1 (en) * 1997-07-24 1999-02-04 The Perkin-Elmer Corporation Conjugates of transporter peptides and nucleic acid analogs, and their use
US6103476A (en) * 1993-11-12 2000-08-15 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled, dual conformation oligonucleotide probes, assays and kits
US20020110826A1 (en) * 2000-07-14 2002-08-15 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof
US20030219375A1 (en) * 1998-06-20 2003-11-27 David Piwnica-Worms Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6103476A (en) * 1993-11-12 2000-08-15 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled, dual conformation oligonucleotide probes, assays and kits
WO1997023607A2 (en) * 1995-12-21 1997-07-03 The University Court Of The University Of Dundee Presentation of antigen, introduced into cells by stimulation of macropinocytosis
WO1999005302A1 (en) * 1997-07-24 1999-02-04 The Perkin-Elmer Corporation Conjugates of transporter peptides and nucleic acid analogs, and their use
US6025140A (en) * 1997-07-24 2000-02-15 Perseptive Biosystems, Inc. Membrane-permeable constructs for transport across a lipid membrane
US20030219375A1 (en) * 1998-06-20 2003-11-27 David Piwnica-Worms Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy
US20020110826A1 (en) * 2000-07-14 2002-08-15 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof

Also Published As

Publication number Publication date
CA2530221A1 (en) 2005-03-10
WO2005021712A2 (en) 2005-03-10
EP1639093A2 (en) 2006-03-29
JP2007525967A (en) 2007-09-13

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