WO2005019449A3 - Expression of a recombinant transgene - Google Patents

Expression of a recombinant transgene Download PDF

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Publication number
WO2005019449A3
WO2005019449A3 PCT/US2004/021451 US2004021451W WO2005019449A3 WO 2005019449 A3 WO2005019449 A3 WO 2005019449A3 US 2004021451 W US2004021451 W US 2004021451W WO 2005019449 A3 WO2005019449 A3 WO 2005019449A3
Authority
WO
WIPO (PCT)
Prior art keywords
heterologous polypeptide
dna sequence
host cell
transgene
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/021451
Other languages
French (fr)
Other versions
WO2005019449A2 (en
Inventor
Richard F Allison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Michigan State University MSU
Original Assignee
Michigan State University MSU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michigan State University MSU filed Critical Michigan State University MSU
Priority to CA002530956A priority Critical patent/CA2530956A1/en
Priority to US10/561,720 priority patent/US7696406B2/en
Publication of WO2005019449A2 publication Critical patent/WO2005019449A2/en
Publication of WO2005019449A3 publication Critical patent/WO2005019449A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A system for expression of a heterologous polypeptide in a transgenic host cell is disclosed. The system is based upon a transgene comprising a eukaryotic promoter operably linked to a DNA sequence comprising, in the 5' to 3' direction, a DNA sequence complementary to a sequence encoding a heterologous polypeptide, a DNA sequence complementary to an internal ribosome entry site, and a DNA sequence corresponding to a 3' untranslated region of a positive strand single-stranded RNA virus. Following introduction of a stimulus, the host cell synthesizes an RNA molecule complementary to a recombinant RNA encoded by the transgene. The stimulus can be a positive strand single-stranded RNA virus or a nucleic acid thereof. Because the complement of the recombinant RNA comprises an internal ribosome entry site and a sequence encoding a heterologous polypeptide, the host cell can synthesize the heterologous polypeptide.
PCT/US2004/021451 2003-07-03 2004-07-02 Expression of a recombinant transgene Ceased WO2005019449A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002530956A CA2530956A1 (en) 2003-07-03 2004-07-02 Expression of a recombinant transgene
US10/561,720 US7696406B2 (en) 2003-07-03 2004-07-02 Expression of a recombinant transgene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48507303P 2003-07-03 2003-07-03
US60/485,073 2003-07-03

Publications (2)

Publication Number Publication Date
WO2005019449A2 WO2005019449A2 (en) 2005-03-03
WO2005019449A3 true WO2005019449A3 (en) 2005-05-06

Family

ID=34215817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/021451 Ceased WO2005019449A2 (en) 2003-07-03 2004-07-02 Expression of a recombinant transgene

Country Status (3)

Country Link
US (1) US7696406B2 (en)
CA (1) CA2530956A1 (en)
WO (1) WO2005019449A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111303251B (en) * 2020-02-25 2021-07-30 中国农业科学院兰州兽医研究所 A method for in vitro assembly of foot-and-mouth disease virus-like particles and its application

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI300441B (en) * 2005-09-21 2008-09-01 Univ Chung Yuan Christian A polynucleotide with ires activity
CN101768591A (en) * 2008-12-31 2010-07-07 中国医学科学院基础医学研究所 Translation adjusting element and application thereof
JP5686399B2 (en) * 2010-02-18 2015-03-18 国立大学法人横浜国立大学 Foreign gene expression elements and their use
US9694068B2 (en) * 2012-03-22 2017-07-04 Pharma Green Llc Methods and compositions to produce vaccines against smallpox in plants
IT201700105372A1 (en) * 2017-09-20 2019-03-20 Fondazione St Italiano Tecnologia FUNCTIONAL NUCLEIC ACID MOLECULE AND ITS USE
CN110885796B (en) * 2018-08-20 2022-04-08 山东农业大学 Attenuated vaccine against potato X virus, preparation method and application thereof
KR20230053998A (en) * 2021-10-15 2023-04-24 (주)지노믹트리 Gene Construct for Expressing mRNA
CN120769918A (en) * 2023-02-14 2025-10-10 伊利克斯根治疗公司 Synthetic mRNA lacking a poly A tail or having a short adenine homopolymer and methods for its use and production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002012522A1 (en) * 2000-08-10 2002-02-14 Plant Bioscience Limited Gene expression from the internal ribosome entry site (ires) of rhopalosiphum padi virus (rhpv)
WO2002029068A2 (en) * 2000-10-06 2002-04-11 Icon Genetics Ag Virus-based amplification vector for plants

Family Cites Families (15)

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US5922602A (en) * 1988-02-26 1999-07-13 Biosource Technologies, Inc. Cytoplasmic inhibition of gene expression
US5854037A (en) * 1989-08-28 1998-12-29 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand RNA virus expression systems and vaccines
US5840520A (en) * 1989-08-28 1998-11-24 Aviron Recombinant negative strand RNA virus expression systems
US5166057A (en) * 1989-08-28 1992-11-24 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand rna virus expression-systems
NL9001711A (en) * 1989-10-03 1991-05-01 Clovis Matton N V GENETIC MANIPULATIONS WITH RECOMBINANT DNA INCLUDING SEQUENCES DERIVED FROM RNA VIRUS.
PT626998E (en) * 1992-02-19 2002-04-29 State Of Oregon By S B Hi Educ "PRODUCTION OF VIRUS RESISTANT PLANTS THROUGH INTRODUCTION OF A NON-TRANSLUCENT VIRAL MOLECULA AND CODIFICATION SENSE"
KR960700344A (en) * 1992-12-30 1996-01-19 데이비드 알. 맥기 Viral AMPLIFICATION OF RECOMBINANT MESSENGER RNA IN TRANSGENIC PLANTS
ATE298370T1 (en) * 1994-04-29 2005-07-15 Baxter Healthcare Sa RECOMBINANT POXVIRUSES WITH FOREIGN POLYNUCLEOTES IN IMPORTANT REGIONS
US6433248B1 (en) * 1998-06-01 2002-08-13 North Carolina State University Trans-activation of transcription from viral RNA
US6270958B1 (en) * 1998-10-29 2001-08-07 Washington University Detection of negative-strand RNA viruses
US6136538A (en) * 1999-01-08 2000-10-24 Washington University Silent inducible virus replicons and uses thereof
US6326480B1 (en) * 1999-01-19 2001-12-04 Signal Pharmaceuticals, Inc. Antisense reporter system for assaying RNA virus replication
WO2000053780A2 (en) * 1999-03-09 2000-09-14 Large Scale Biology Corporation Multiple component rna vector system for expression of foreign sequences
EP1196614A1 (en) 1999-06-24 2002-04-17 Metabolix, Inc. Plant multi-gene expression constructs
CA2459915A1 (en) 2001-09-07 2003-03-20 Board Of Trustees Operating Michigan State University Blueberry red ringspot virus, sequences, promoters, and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002012522A1 (en) * 2000-08-10 2002-02-14 Plant Bioscience Limited Gene expression from the internal ribosome entry site (ires) of rhopalosiphum padi virus (rhpv)
WO2002029068A2 (en) * 2000-10-06 2002-04-11 Icon Genetics Ag Virus-based amplification vector for plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TOTH R L ET AL: "A novel strategy for the expression of foreign genes from plant virus vectors", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 489, no. 2-3, 2 February 2001 (2001-02-02), pages 215 - 219, XP004248888, ISSN: 0014-5793 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111303251B (en) * 2020-02-25 2021-07-30 中国农业科学院兰州兽医研究所 A method for in vitro assembly of foot-and-mouth disease virus-like particles and its application

Also Published As

Publication number Publication date
CA2530956A1 (en) 2005-03-03
US7696406B2 (en) 2010-04-13
WO2005019449A2 (en) 2005-03-03
US20070136890A1 (en) 2007-06-14

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